AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar ZhongmuNo.1 / MsG0280006731.01

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MsG0280006731.01.T01 MTR_2g013780 97.241 145 4 0 110 254 173 317 6.92e-104 304
MsG0280006731.01.T01 MTR_2g013780 87.879 99 11 1 1 98 1 99 5.84e-53 174
MsG0280006731.01.T01 MTR_2g013880 76.871 147 32 2 110 254 173 319 5.31e-79 241
MsG0280006731.01.T01 MTR_2g013880 61.702 94 35 1 1 94 4 96 3.11e-28 110
MsG0280006731.01.T01 MTR_4g088770 42.462 325 115 8 1 254 1 324 9.53e-72 223
MsG0280006731.01.T01 MTR_4g088760 66.438 146 47 2 110 254 232 376 1.18e-66 211
MsG0280006731.01.T01 MTR_4g078310 59.184 147 53 5 110 254 149 290 4.37e-54 176
MsG0280006731.01.T01 MTR_5g008720 42.982 114 63 1 110 221 127 240 4.19e-29 110
MsG0280006731.01.T01 MTR_8g093440 36.937 111 65 2 110 219 112 218 7.98e-24 97.1
MsG0280006731.01.T01 MTR_8g093440 36.937 111 65 2 110 219 50 156 1.05e-23 95.5
MsG0280006731.01.T01 MTR_8g012240 37.500 112 66 2 110 217 161 272 2.69e-23 96.3
MsG0280006731.01.T01 MTR_1g041155 42.268 97 50 2 110 206 183 273 1.51e-22 95.1
MsG0280006731.01.T01 MTR_7g095960 40.566 106 57 1 106 211 186 285 3.27e-22 94.4
MsG0280006731.01.T01 MTR_7g053310 35.833 120 72 3 110 225 151 269 3.72e-22 93.2
MsG0280006731.01.T01 MTR_4g132963 40.777 103 55 1 110 212 145 241 8.03e-22 92.0
MsG0280006731.01.T01 MTR_4g132940 40.777 103 55 1 110 212 145 241 8.03e-22 92.0
MsG0280006731.01.T01 MTR_1g076500 39.815 108 59 2 110 217 205 306 1.13e-21 93.2
MsG0280006731.01.T01 MTR_4g132970 40.777 103 55 1 110 212 166 262 1.28e-21 92.0
MsG0280006731.01.T01 MTR_7g083080 37.500 104 64 1 110 213 161 263 4.86e-21 90.5
MsG0280006731.01.T01 MTR_2g025520 38.095 105 59 2 110 214 164 262 1.33e-20 89.4
MsG0280006731.01.T01 MTR_1g070970 34.459 148 88 3 110 254 198 339 3.12e-20 88.6
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MsG0280006731.01.T01 AT4G32480 61.832 131 48 2 110 240 146 274 4.56e-53 174
MsG0280006731.01.T01 AT2G20670 55.147 136 53 2 110 240 148 280 3.70e-49 164
MsG0280006731.01.T01 AT3G07350 38.739 111 68 0 110 220 165 275 2.32e-29 112
MsG0280006731.01.T01 AT4G14620 36.986 146 84 3 110 253 173 312 2.94e-26 105
MsG0280006731.01.T01 AT3G25240 38.679 106 64 1 110 214 145 250 7.93e-24 97.4
MsG0280006731.01.T01 AT2G38820 43.158 95 48 2 110 204 189 277 3.96e-23 96.3
MsG0280006731.01.T01 AT3G22970 41.176 102 54 1 110 211 87 182 1.97e-22 93.2
MsG0280006731.01.T01 AT3G22970 43.158 95 48 1 110 204 198 286 3.33e-21 91.7
MsG0280006731.01.T01 AT3G54550 37.500 104 59 1 110 213 178 275 4.68e-19 84.7
MsG0280006731.01.T01 AT1G62420 35.135 111 69 1 108 218 136 243 4.88e-19 84.3
MsG0280006731.01.T01 AT2G39650 31.683 101 69 0 110 210 140 240 2.08e-18 82.8
MsG0280006731.01.T01 AT2G39650 31.683 101 69 0 110 210 78 178 2.10e-18 81.6
MsG0280006731.01.T01 AT1G12030 36.792 106 60 2 108 212 138 237 1.52e-17 80.5
MsG0280006731.01.T01 AT1G77145 35.238 105 64 1 112 212 115 219 8.27e-17 77.8
MsG0280006731.01.T01 AT1G77160 42.667 75 43 0 130 204 87 161 1.06e-16 76.6
MsG0280006731.01.T01 AT1G77160 40.964 83 48 1 130 211 142 224 1.50e-16 77.4
MsG0280006731.01.T01 AT1G77145 35.238 105 64 1 112 212 58 162 1.98e-16 75.9

Find 61 sgRNAs with CRISPR-Local

Find 154 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
CATGTTGACTATTGATTTAT+TGG 0.144388 2:+5476811 MsG0280006731.01.T01:CDS
AACATGGATGCCTTTGATTT+TGG 0.176314 2:-5476794 None:intergenic
ATCCTTACTTCCCCTTTCTT+TGG 0.194440 2:-5476482 None:intergenic
CAGGAACCTAGTAGACTTTC+TGG 0.231900 2:-5474420 None:intergenic
TCATTCACTTGAAATGGATT+TGG 0.300841 2:+5474577 MsG0280006731.01.T01:CDS
TTGAGAAGAACCGAAGCTTT+TGG 0.320088 2:+5474636 MsG0280006731.01.T01:CDS
GTGATATATGCTATTAGATT+AGG 0.351596 2:-5476407 None:intergenic
TCTATGTCATTCACTTGAAA+TGG 0.356046 2:+5474571 MsG0280006731.01.T01:CDS
ATTGCATTGCACAGCTGTTA+AGG 0.385921 2:+5476847 MsG0280006731.01.T01:CDS
ATAGGTACATGCAAGCTAAA+TGG 0.395692 2:+5476699 MsG0280006731.01.T01:CDS
TGTTGGGAAAGTAGAGAGAT+TGG 0.411917 2:+5476595 MsG0280006731.01.T01:CDS
ATCCAAAGAAAGGGGAAGTA+AGG 0.437384 2:+5476480 MsG0280006731.01.T01:CDS
GACAATACAAATCCAAAGAA+AGG 0.438456 2:+5476470 MsG0280006731.01.T01:CDS
AGCTGCCTGAGGTGTTTGTT+GGG 0.442109 2:+5476579 MsG0280006731.01.T01:CDS
CTTTGAGAAAACAAGAATCA+TGG 0.448459 2:+5474382 MsG0280006731.01.T01:exon
CCTTGGCCAGAAAGTCTACT+AGG 0.449081 2:+5474414 MsG0280006731.01.T01:CDS
TATATCACAATTAACTATGC+AGG 0.453523 2:+5476422 MsG0280006731.01.T01:CDS
GTTCCTGACGATTAATCGTC+AGG 0.459884 2:+5474436 MsG0280006731.01.T01:CDS
AAAGCTTCGGTTCTTCTCAA+TGG 0.462467 2:-5474633 None:intergenic
GTTGGCTTTCCCAAAAGCTT+CGG 0.463303 2:-5474646 None:intergenic
ACAATACAAATCCAAAGAAA+GGG 0.465593 2:+5476471 MsG0280006731.01.T01:CDS
GGATGATGATGAAGAAGAAA+GGG 0.467348 2:+5474598 MsG0280006731.01.T01:CDS
AAGCTGCCTGAGGTGTTTGT+TGG 0.477018 2:+5476578 MsG0280006731.01.T01:CDS
GTTGGGAAAGTAGAGAGATT+GGG 0.477670 2:+5476596 MsG0280006731.01.T01:CDS
TGGATGATGATGAAGAAGAA+AGG 0.481696 2:+5474597 MsG0280006731.01.T01:CDS
AAGCCAACATCTTGGTGTGC+AGG 0.494557 2:+5474661 MsG0280006731.01.T01:CDS
TATCCTGACGATTAATCGTC+AGG 0.500747 2:-5474439 None:intergenic
TTCAACAACTCCTCTTCCAA+TGG 0.503995 2:+5476745 MsG0280006731.01.T01:CDS
TACATGCAAGCTAAATGGTT+GGG 0.506802 2:+5476704 MsG0280006731.01.T01:CDS
CAATAAATCAATAGTCAACA+TGG 0.512237 2:-5476810 None:intergenic
TTGCCATCTCAAACTCAGCT+TGG 0.517950 2:-5476523 None:intergenic
AAGCTGAGTTTGAGATGGCA+AGG 0.523389 2:+5476525 MsG0280006731.01.T01:CDS
TCAACAACTCCTCTTCCAAT+GGG 0.527252 2:+5476746 MsG0280006731.01.T01:CDS
TTTGAGAAAACAAGAATCAT+GGG 0.534040 2:+5474383 MsG0280006731.01.T01:exon
AATCATGGGTGTAACCTCCT+TGG 0.536053 2:+5474397 MsG0280006731.01.T01:exon
ATATCACAATTAACTATGCA+GGG 0.542851 2:+5476423 MsG0280006731.01.T01:CDS
ACTGACGAAGTGATCATGTC+GGG 0.551370 2:+5474527 MsG0280006731.01.T01:CDS
TCAGAAATTCCCATTGGAAG+AGG 0.553074 2:-5476755 None:intergenic
AATGGTTGGGTCCATGTGAA+AGG 0.556150 2:+5476717 MsG0280006731.01.T01:CDS
ATGTACCTATGCTTCCTCCA+TGG 0.558080 2:-5476686 None:intergenic
TTTCCAAGCTGAGTTTGAGA+TGG 0.565289 2:+5476520 MsG0280006731.01.T01:CDS
AATAACAAAGCCAAAATCAA+AGG 0.568849 2:+5476784 MsG0280006731.01.T01:CDS
GTTGAAGTGTTCCTTTCACA+TGG 0.569816 2:-5476728 None:intergenic
GTACATGCAAGCTAAATGGT+TGG 0.573929 2:+5476703 MsG0280006731.01.T01:CDS
ATCCTCTCAGAAATTCCCAT+TGG 0.574010 2:-5476761 None:intergenic
ACAGCTGTGCAATGCAATGT+TGG 0.576393 2:-5476842 None:intergenic
TGGGGCCATGGAGGAAGCAT+AGG 0.577510 2:+5476681 MsG0280006731.01.T01:CDS
AGCTGAGTTTGAGATGGCAA+GGG 0.587593 2:+5476526 MsG0280006731.01.T01:CDS
CCTAGTAGACTTTCTGGCCA+AGG 0.588067 2:-5474414 None:intergenic
ACTTTCCCAACAAACACCTC+AGG 0.590533 2:-5476584 None:intergenic
CAGGATAGTCTCGAGTCTGA+AGG 0.595130 2:+5474455 MsG0280006731.01.T01:CDS
TCACCTGCACACCAAGATGT+TGG 0.598265 2:-5474664 None:intergenic
CAATCTTCAGACACGAGTGT+TGG 0.608841 2:+5474479 MsG0280006731.01.T01:CDS
TACTGACGAAGTGATCATGT+CGG 0.631088 2:+5474526 MsG0280006731.01.T01:CDS
CAATACAAATCCAAAGAAAG+GGG 0.639538 2:+5476472 MsG0280006731.01.T01:CDS
AAATGCACATGGGGCCATGG+AGG 0.645712 2:+5476672 MsG0280006731.01.T01:CDS
GCTGAGTTTGAGATGGCAAG+GGG 0.658083 2:+5476527 MsG0280006731.01.T01:CDS
TTCCAATGGGAATTTCTGAG+AGG 0.662878 2:+5476759 MsG0280006731.01.T01:CDS
AGTAGACTTTCTGGCCAAGG+AGG 0.667212 2:-5474411 None:intergenic
ACTCGTGCAGAAGCTGCCTG+AGG 0.680427 2:+5476568 MsG0280006731.01.T01:CDS
TATCACAATTAACTATGCAG+GGG 0.694217 2:+5476424 MsG0280006731.01.T01:CDS

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!! ACTAAAATTAACTTAAAAAT+AGG + Chr2:5475819-5475838 MsG0280006731.01.T01:intron 10.0%
!! CTAAAATTAACTTAAAAATA+GGG + Chr2:5475820-5475839 MsG0280006731.01.T01:intron 10.0%
!! TTAAATCATATTTATTAAGA+AGG - Chr2:5474924-5474943 None:intergenic 10.0%
!! GGATTAAAAAATTAAAAAGT+AGG + Chr2:5475998-5476017 MsG0280006731.01.T01:intron 15.0%
!! TCCAAAATAAAATAAAAAAG+TGG + Chr2:5475951-5475970 MsG0280006731.01.T01:intron 15.0%
!!! ACCACTTTTTTATTTTATTT+TGG - Chr2:5475955-5475974 None:intergenic 15.0%
!!! ATATGAGTTTACTTTAAAAA+GGG - Chr2:5475566-5475585 None:intergenic 15.0%
!!! CAAATACTACATATTAATTT+TGG - Chr2:5474793-5474812 None:intergenic 15.0%
!!! GAAAAGTTGATATATTTATA+GGG + Chr2:5475911-5475930 MsG0280006731.01.T01:intron 15.0%
!!! TAGTGATTGAAAATTTTATA+GGG + Chr2:5475851-5475870 MsG0280006731.01.T01:intron 15.0%
!!! TTTTGGATTAAATATGTTTT+TGG - Chr2:5475938-5475957 None:intergenic 15.0%
!!! AAAAGGGTTAAATATGTTTT+TGG + Chr2:5475448-5475467 MsG0280006731.01.T01:intron 20.0%
!!! AAATTTTCAATCACTACTTT+TGG - Chr2:5475847-5475866 None:intergenic 20.0%
!!! AAGTTGAAAGAAAATTTTAG+AGG - Chr2:5475507-5475526 None:intergenic 20.0%
!!! AGTTGAAAGAAAATTTTAGA+GGG - Chr2:5475506-5475525 None:intergenic 20.0%
!!! CATATGAGTTTACTTTAAAA+AGG - Chr2:5475567-5475586 None:intergenic 20.0%
!!! CATTGCATAATATTTTGATT+AGG - Chr2:5476625-5476644 None:intergenic 20.0%
!!! CGAAAAGTTGATATATTTAT+AGG + Chr2:5475910-5475929 MsG0280006731.01.T01:intron 20.0%
!!! GAAAAGTTGGTATATTTATA+GGG - Chr2:5475475-5475494 None:intergenic 20.0%
!!! GTAGTATTTGATTTTTTTGT+TGG + Chr2:5474803-5474822 MsG0280006731.01.T01:intron 20.0%
!!! GTAGTGATTGAAAATTTTAT+AGG + Chr2:5475850-5475869 MsG0280006731.01.T01:intron 20.0%
!!! TAACCCTTTTATTTTTAAGT+GGG - Chr2:5475438-5475457 None:intergenic 20.0%
!!! TGAACTTGTTCTATATATAT+TGG - Chr2:5475025-5475044 None:intergenic 20.0%
!!! TTAACCCTTTTATTTTTAAG+TGG - Chr2:5475439-5475458 None:intergenic 20.0%
!!! TTTTATGAAAAGCAATATTG+TGG + Chr2:5475353-5475372 MsG0280006731.01.T01:intron 20.0%
!!! TTTTGTTTTGATCAATCAAT+GGG + Chr2:5474981-5475000 MsG0280006731.01.T01:intron 20.0%
! AAGGATAAAAAAATGCACAT+GGG + Chr2:5476662-5476681 MsG0280006731.01.T01:CDS 25.0%
! AATAACAAAGCCAAAATCAA+AGG + Chr2:5476784-5476803 MsG0280006731.01.T01:CDS 25.0%
! ACAATACAAATCCAAAGAAA+GGG + Chr2:5476471-5476490 MsG0280006731.01.T01:CDS 25.0%
! AGCACATTAACTACATATAA+AGG - Chr2:5476360-5476379 None:intergenic 25.0%
! ATATCACAATTAACTATGCA+GGG + Chr2:5476423-5476442 MsG0280006731.01.T01:CDS 25.0%
! ATTCAAGAAATAAAAAGCTG+TGG + Chr2:5475081-5475100 MsG0280006731.01.T01:intron 25.0%
! CAATAAATCAATAGTCAACA+TGG - Chr2:5476813-5476832 None:intergenic 25.0%
! CTATAATTATCAGCTAAGTA+CGG + Chr2:5474862-5474881 MsG0280006731.01.T01:intron 25.0%
! GGAAAGAAAGAAAATTGAAA+AGG + Chr2:5476040-5476059 MsG0280006731.01.T01:intron 25.0%
! GTGATATATGCTATTAGATT+AGG - Chr2:5476410-5476429 None:intergenic 25.0%
! TATATCACAATTAACTATGC+AGG + Chr2:5476422-5476441 MsG0280006731.01.T01:CDS 25.0%
! TATTTCTTGAATAGCTTCTT+TGG - Chr2:5475073-5475092 None:intergenic 25.0%
! TCACCCACTTAAAAATAAAA+GGG + Chr2:5475432-5475451 MsG0280006731.01.T01:intron 25.0%
! TCATGAATTTAAGTCTACTT+TGG - Chr2:5476286-5476305 None:intergenic 25.0%
! TTCACCCACTTAAAAATAAA+AGG + Chr2:5475431-5475450 MsG0280006731.01.T01:intron 25.0%
! TTTGAGAAAACAAGAATCAT+GGG + Chr2:5474383-5474402 MsG0280006731.01.T01:exon 25.0%
!! AAATTTTCCATCTTCACTTT+TGG + Chr2:5475539-5475558 MsG0280006731.01.T01:intron 25.0%
!! AAGTTGAAGGAAAATTTTAG+AGG + Chr2:5475879-5475898 MsG0280006731.01.T01:intron 25.0%
!! AGTTGAAGGAAAATTTTAGA+GGG + Chr2:5475880-5475899 MsG0280006731.01.T01:intron 25.0%
!! ATTAGTGTGATTTTATGTTG+AGG + Chr2:5474723-5474742 MsG0280006731.01.T01:intron 25.0%
!! CACACTATTCTTTTATAGAT+TGG - Chr2:5476316-5476335 None:intergenic 25.0%
!! TGAAGATGGAAAATTTTTGA+AGG - Chr2:5475535-5475554 None:intergenic 25.0%
!! TTAGTGTGATTTTATGTTGA+GGG + Chr2:5474724-5474743 MsG0280006731.01.T01:intron 25.0%
!!! CAATCAATGGGATTTAATTT+TGG + Chr2:5474993-5475012 MsG0280006731.01.T01:intron 25.0%
!!! CATGTTGACTATTGATTTAT+TGG + Chr2:5476811-5476830 MsG0280006731.01.T01:CDS 25.0%
!!! CGAAAAGTTGGTATATTTAT+AGG - Chr2:5475476-5475495 None:intergenic 25.0%
!!! CTTTTGTTTTGATCAATCAA+TGG + Chr2:5474980-5474999 MsG0280006731.01.T01:intron 25.0%
!!! GTTCTAAAAGCATTAGATTT+TGG + Chr2:5474954-5474973 MsG0280006731.01.T01:intron 25.0%
!!! TATTTTTAAGTGGGTGAAAA+GGG - Chr2:5475429-5475448 None:intergenic 25.0%
!!! TTATTTTTAAGTGGGTGAAA+AGG - Chr2:5475430-5475449 None:intergenic 25.0%
!!! TTTATCCTTCATGCATTTTT+TGG - Chr2:5476651-5476670 None:intergenic 25.0%
AATGAATAACATGTACCTGA+TGG - Chr2:5475252-5475271 None:intergenic 30.0%
ACGTTAAAAGTTTCAGATTG+AGG - Chr2:5476107-5476126 None:intergenic 30.0%
AGGATAAAAAAATGCACATG+GGG + Chr2:5476663-5476682 MsG0280006731.01.T01:CDS 30.0%
ATGAAAAGCAATATTGTGGT+GGG + Chr2:5475357-5475376 MsG0280006731.01.T01:intron 30.0%
CAATACAAATCCAAAGAAAG+GGG + Chr2:5476472-5476491 MsG0280006731.01.T01:CDS 30.0%
GAAGGATAAAAAAATGCACA+TGG + Chr2:5476661-5476680 MsG0280006731.01.T01:CDS 30.0%
GAATTTAAGTCTACTTTGGT+TGG - Chr2:5476282-5476301 None:intergenic 30.0%
GACAATACAAATCCAAAGAA+AGG + Chr2:5476470-5476489 MsG0280006731.01.T01:CDS 30.0%
GGAAATGAATAGATTCTTCA+TGG + Chr2:5476019-5476038 MsG0280006731.01.T01:intron 30.0%
GTTCATTAGAGACAAAGATA+AGG + Chr2:5475040-5475059 MsG0280006731.01.T01:intron 30.0%
TATCACAATTAACTATGCAG+GGG + Chr2:5476424-5476443 MsG0280006731.01.T01:CDS 30.0%
TATGAAAAGCAATATTGTGG+TGG + Chr2:5475356-5475375 MsG0280006731.01.T01:intron 30.0%
TCTATGTCATTCACTTGAAA+TGG + Chr2:5474571-5474590 MsG0280006731.01.T01:CDS 30.0%
TTATAGGGACTAAAAGTTGA+AGG + Chr2:5475866-5475885 MsG0280006731.01.T01:intron 30.0%
! CTTTGAGAAAACAAGAATCA+TGG + Chr2:5474382-5474401 MsG0280006731.01.T01:exon 30.0%
! TCATTCACTTGAAATGGATT+TGG + Chr2:5474577-5474596 MsG0280006731.01.T01:CDS 30.0%
!! GTGCTATTTGCAAAACAAAA+TGG + Chr2:5475208-5475227 MsG0280006731.01.T01:intron 30.0%
!!! CTAAAAGCATTAGATTTTGG+AGG + Chr2:5474957-5474976 MsG0280006731.01.T01:intron 30.0%
ACTTCATCAGATATTCCATC+AGG + Chr2:5475234-5475253 MsG0280006731.01.T01:intron 35.0%
ATAGGTACATGCAAGCTAAA+TGG + Chr2:5476699-5476718 MsG0280006731.01.T01:CDS 35.0%
TACATGCAAGCTAAATGGTT+GGG + Chr2:5476704-5476723 MsG0280006731.01.T01:CDS 35.0%
TTCTTGAATAGCTTCTTTGG+TGG - Chr2:5475070-5475089 None:intergenic 35.0%
! AAGTCTACTTTGGTTGGTAT+TGG - Chr2:5476276-5476295 None:intergenic 35.0%
! GGATGATGATGAAGAAGAAA+GGG + Chr2:5474598-5474617 MsG0280006731.01.T01:CDS 35.0%
! TGGATGATGATGAAGAAGAA+AGG + Chr2:5474597-5474616 MsG0280006731.01.T01:CDS 35.0%
!! AACATGGATGCCTTTGATTT+TGG - Chr2:5476797-5476816 None:intergenic 35.0%
AAAGCTTCGGTTCTTCTCAA+TGG - Chr2:5474636-5474655 None:intergenic 40.0%
AAAGGGACCAAAAGTGAAGA+TGG - Chr2:5475549-5475568 None:intergenic 40.0%
AATGATGCTCTTGTGGTAAG+AGG + Chr2:5475976-5475995 MsG0280006731.01.T01:intron 40.0%
ACTTCACTCTATCGACATAG+AGG + Chr2:5476238-5476257 MsG0280006731.01.T01:intron 40.0%
ATCCAAAGAAAGGGGAAGTA+AGG + Chr2:5476480-5476499 MsG0280006731.01.T01:CDS 40.0%
ATCCTCTCAGAAATTCCCAT+TGG - Chr2:5476764-5476783 None:intergenic 40.0%
ATCCTTACTTCCCCTTTCTT+TGG - Chr2:5476485-5476504 None:intergenic 40.0%
ATGATGCTCTTGTGGTAAGA+GGG + Chr2:5475977-5475996 MsG0280006731.01.T01:intron 40.0%
ATTGCATTGCACAGCTGTTA+AGG + Chr2:5476847-5476866 MsG0280006731.01.T01:CDS 40.0%
CTGCAAAAACTGCTTGATGA+GGG + Chr2:5475149-5475168 MsG0280006731.01.T01:intron 40.0%
CTTCACTCTATCGACATAGA+GGG + Chr2:5476239-5476258 MsG0280006731.01.T01:intron 40.0%
GAGGGACTAAAACGAAAAGT+TGG - Chr2:5475917-5475898 None:intergenic 40.0%
GGTTGCAACAGAAAGATGAT+GGG + Chr2:5475114-5475133 MsG0280006731.01.T01:intron 40.0%
GTACATGCAAGCTAAATGGT+TGG + Chr2:5476703-5476722 MsG0280006731.01.T01:CDS 40.0%
GTTGGGAAAGTAGAGAGATT+GGG + Chr2:5476596-5476615 MsG0280006731.01.T01:CDS 40.0%
TACTGACGAAGTGATCATGT+CGG + Chr2:5474526-5474545 MsG0280006731.01.T01:CDS 40.0%
TATCCTGACGATTAATCGTC+AGG - Chr2:5474442-5474461 None:intergenic 40.0%
TCAACAACTCCTCTTCCAAT+GGG + Chr2:5476746-5476765 MsG0280006731.01.T01:CDS 40.0%
TCAGAAATTCCCATTGGAAG+AGG - Chr2:5476758-5476777 None:intergenic 40.0%
TCTATGAGGCTTCAGAAATC+TGG + Chr2:5475177-5475196 MsG0280006731.01.T01:intron 40.0%
TGATGAGGGAAGTTTCTATG+AGG + Chr2:5475163-5475182 MsG0280006731.01.T01:intron 40.0%
TGCAGCCAAAAAATGCATGA+AGG + Chr2:5476643-5476662 MsG0280006731.01.T01:CDS 40.0%
TGGTTGCAACAGAAAGATGA+TGG + Chr2:5475113-5475132 MsG0280006731.01.T01:intron 40.0%
TGTTGGGAAAGTAGAGAGAT+TGG + Chr2:5476595-5476614 MsG0280006731.01.T01:CDS 40.0%
TTCAACAACTCCTCTTCCAA+TGG + Chr2:5476745-5476764 MsG0280006731.01.T01:CDS 40.0%
TTCCAATGGGAATTTCTGAG+AGG + Chr2:5476759-5476778 MsG0280006731.01.T01:CDS 40.0%
TTTCCAAGCTGAGTTTGAGA+TGG + Chr2:5476520-5476539 MsG0280006731.01.T01:CDS 40.0%
! GTTGAAGTGTTCCTTTCACA+TGG - Chr2:5476731-5476750 None:intergenic 40.0%
! TGAGAAGAACCGAAGCTTTT+GGG + Chr2:5474637-5474656 MsG0280006731.01.T01:CDS 40.0%
! TTGAGAAGAACCGAAGCTTT+TGG + Chr2:5474636-5474655 MsG0280006731.01.T01:CDS 40.0%
!! TGTGGTGGGTCTAATAAGAT+AGG + Chr2:5475371-5475390 MsG0280006731.01.T01:intron 40.0%
!! TTTTGGGAAAGCCAACATCT+TGG + Chr2:5474653-5474672 MsG0280006731.01.T01:CDS 40.0%
AAAAAATGCACATGGGGCCA+TGG + Chr2:5476669-5476688 MsG0280006731.01.T01:CDS 45.0%
AATCATGGGTGTAACCTCCT+TGG + Chr2:5474397-5474416 MsG0280006731.01.T01:exon 45.0%
AATGGTTGGGTCCATGTGAA+AGG + Chr2:5476717-5476736 MsG0280006731.01.T01:CDS 45.0%
ACAGCTGTGCAATGCAATGT+TGG - Chr2:5476845-5476864 None:intergenic 45.0%
ACTGACGAAGTGATCATGTC+GGG + Chr2:5474527-5474546 MsG0280006731.01.T01:CDS 45.0%
ACTTTCCCAACAAACACCTC+AGG - Chr2:5476587-5476606 None:intergenic 45.0%
AGTGGTGAATGATGCTCTTG+TGG + Chr2:5475969-5475988 MsG0280006731.01.T01:intron 45.0%
ATGTACCTATGCTTCCTCCA+TGG - Chr2:5476689-5476708 None:intergenic 45.0%
CAATCTTCAGACACGAGTGT+TGG + Chr2:5474479-5474498 MsG0280006731.01.T01:CDS 45.0%
CAGGAACCTAGTAGACTTTC+TGG - Chr2:5474423-5474442 None:intergenic 45.0%
CCTGCAAAAACTGCTTGATG+AGG + Chr2:5475148-5475167 MsG0280006731.01.T01:intron 45.0%
GTTCCTGACGATTAATCGTC+AGG + Chr2:5474436-5474455 MsG0280006731.01.T01:CDS 45.0%
TCGATAGAGTGAAGTCATCG+CGG - Chr2:5476233-5476252 None:intergenic 45.0%
TTGCCATCTCAAACTCAGCT+TGG - Chr2:5476526-5476545 None:intergenic 45.0%
! AAGCTGAGTTTGAGATGGCA+AGG + Chr2:5476525-5476544 MsG0280006731.01.T01:CDS 45.0%
! AGCTGAGTTTGAGATGGCAA+GGG + Chr2:5476526-5476545 MsG0280006731.01.T01:CDS 45.0%
! CATCAAGCAGTTTTTGCAGG+TGG - Chr2:5475148-5475167 None:intergenic 45.0%
! CCTCATCAAGCAGTTTTTGC+AGG - Chr2:5475151-5475170 None:intergenic 45.0%
! TGCAGGGGAACACACATTTT+TGG + Chr2:5476439-5476458 MsG0280006731.01.T01:CDS 45.0%
!! GTTGGCTTTCCCAAAAGCTT+CGG - Chr2:5474649-5474668 None:intergenic 45.0%
!!! AAAAAATTCTAAATTTTTTT+GGG - Chr2:5475650-5475669 None:intergenic 5.0%
!!! ATGAATTTTTATATATTTTA+TGG + Chr2:5475689-5475708 MsG0280006731.01.T01:intron 5.0%
!!! TAAAAAATTCTAAATTTTTT+TGG - Chr2:5475651-5475670 None:intergenic 5.0%
!!! TAAAAAATTCTAATTTTTTT+TGG + Chr2:5475742-5475761 MsG0280006731.01.T01:intron 5.0%
!!! TGAATTTTTAATATTATATA+TGG + Chr2:5474692-5474711 MsG0280006731.01.T01:intron 5.0%
AAAAGCTGTGGAGCAGTGTG+TGG + Chr2:5475093-5475112 MsG0280006731.01.T01:intron 50.0%
CAGGATAGTCTCGAGTCTGA+AGG + Chr2:5474455-5474474 MsG0280006731.01.T01:CDS 50.0%
CCTTGGCCAGAAAGTCTACT+AGG + Chr2:5474414-5474433 MsG0280006731.01.T01:CDS 50.0%
TCACCTGCACACCAAGATGT+TGG - Chr2:5474667-5474686 None:intergenic 50.0%
! AAGCCAACATCTTGGTGTGC+AGG + Chr2:5474661-5474680 MsG0280006731.01.T01:CDS 50.0%
! AAGCTGCCTGAGGTGTTTGT+TGG + Chr2:5476578-5476597 MsG0280006731.01.T01:CDS 50.0%
! AGCTGCCTGAGGTGTTTGTT+GGG + Chr2:5476579-5476598 MsG0280006731.01.T01:CDS 50.0%
! AGTAGACTTTCTGGCCAAGG+AGG - Chr2:5474414-5474433 None:intergenic 50.0%
! CCTAGTAGACTTTCTGGCCA+AGG - Chr2:5474417-5474436 None:intergenic 50.0%
! GCAGTTTTTGCAGGTGGTGA+TGG - Chr2:5475142-5475161 None:intergenic 50.0%
! GCTGAGTTTGAGATGGCAAG+GGG + Chr2:5476527-5476546 MsG0280006731.01.T01:CDS 50.0%
AAATGCACATGGGGCCATGG+AGG + Chr2:5476672-5476691 MsG0280006731.01.T01:CDS 55.0%
ACTCGTGCAGAAGCTGCCTG+AGG + Chr2:5476568-5476587 MsG0280006731.01.T01:CDS 60.0%
! TGGGGCCATGGAGGAAGCAT+AGG + Chr2:5476681-5476700 MsG0280006731.01.T01:CDS 60.0%
Chromosome Type Strat End Strand Name
Chr2 gene 5474360 5476877 5474360 ID=MsG0280006731.01;Name=MsG0280006731.01
Chr2 mRNA 5474360 5476877 5474360 ID=MsG0280006731.01.T01;Parent=MsG0280006731.01;Name=MsG0280006731.01.T01;_AED=0.37;_eAED=0.40;_QI=41|0|0.5|1|0|0|2|0|254
Chr2 exon 5474360 5474682 5474360 ID=MsG0280006731.01.T01:exon:2207;Parent=MsG0280006731.01.T01
Chr2 exon 5476395 5476877 5476395 ID=MsG0280006731.01.T01:exon:2208;Parent=MsG0280006731.01.T01
Chr2 five_prime_UTR 5474360 5474400 5474360 ID=MsG0280006731.01.T01:five_prime_utr;Parent=MsG0280006731.01.T01
Chr2 CDS 5474401 5474682 5474401 ID=MsG0280006731.01.T01:cds;Parent=MsG0280006731.01.T01
Chr2 CDS 5476395 5476877 5476395 ID=MsG0280006731.01.T01:cds;Parent=MsG0280006731.01.T01
Gene Sequence

>MsG0280006731.01.T01

ATGGGTGTAACCTCCTTGGCCAGAAAGTCTACTAGGTTCCTGACGATTAATCGTCAGGATAGTCTCGAGTCTGAAGGACAATCTTCAGACACGAGTGTTGGTGATGTTGAGTTTGAATTTCTTAATACTGACGAAGTGATCATGTCGGGTCAAAGTGCTAGTAGTGATGATCTATGTCATTCACTTGAAATGGATTTGGATGATGATGAAGAAGAAAGGGATTCAAGTGATACCATTGAGAAGAACCGAAGCTTTTGGGAAAGCCAACATCTTGGTGTGCAGTATCATAATTTTCCTAATCTAATAGCATATATCACAATTAACTATGCAGGGGAACACACATTTTTGGATGTGATAGACAATACAAATCCAAAGAAAGGGGAAGTAAGGATCATGATTGAGCTGAATTTCCAAGCTGAGTTTGAGATGGCAAGGGGAAGTGATGAATACAATAAACTCGTGCAGAAGCTGCCTGAGGTGTTTGTTGGGAAAGTAGAGAGATTGGGCAACCTAATCAAAATATTATGCAATGCAGCCAAAAAATGCATGAAGGATAAAAAAATGCACATGGGGCCATGGAGGAAGCATAGGTACATGCAAGCTAAATGGTTGGGTCCATGTGAAAGGAACACTTCAACAACTCCTCTTCCAATGGGAATTTCTGAGAGGATAATAACAAAGCCAAAATCAAAGGCATCCATGTTGACTATTGATTTATTGGAGAAGCTTCCAACATTGCATTGCACAGCTGTTAAGGTTGTGTGA
Protein sequence

>MsG0280006731.01.T01

MGVTSLARKSTRFLTINRQDSLESEGQSSDTSVGDVEFEFLNTDEVIMSGQSASSDDLCHSLEMDLDDDEEERDSSDTIEKNRSFWESQHLGVQYHNFPNLIAYITINYAGEHTFLDVIDNTNPKKGEVRIMIELNFQAEFEMARGSDEYNKLVQKLPEVFVGKVERLGNLIKILCNAAKKCMKDKKMHMGPWRKHRYMQAKWLGPCERNTSTTPLPMGISERIITKPKSKASMLTIDLLEKLPTLHCTAVKVV*