Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MsG0280006752.01.T01 | XP_003593626.1 | 73.75 | 80 | 21 | 0 | 2 | 81 | 24 | 103 | 8.72E-30 | 114 |
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MsG0280006752.01.T01 | Q94ID6 | 62.295 | 61 | 18 | 1 | 2 | 62 | 21 | 76 | 2.22E-15 | 70.1 |
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MsG0280006752.01.T01 | G7ISE4 | 73.750 | 80 | 21 | 0 | 2 | 81 | 24 | 103 | 4.17e-30 | 114 |
| Gene ID | Type | Classification |
|---|---|---|
| MsG0280006752.01.T01 | TF | AP2/ERF-ERF |
| Gene ID | Type | Classification |
|---|
Co-expression Network
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|---|---|---|---|
| MsG0280006752.01 | MsG0280007667.01 | 0.821518 | 3.870216e-53 | 3.155545e-50 |
| MsG0280006752.01 | MsG0480023698.01 | 0.802791 | 4.744010e-49 | 2.344952e-46 |
PPI
| Gene1 | Gene2 | Type |
|---|
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MsG0280006752.01.T01 | MTR_2g014300 | 73.750 | 80 | 21 | 0 | 2 | 81 | 24 | 103 | 1.06e-33 | 114 |
| MsG0280006752.01.T01 | MTR_5g085070 | 59.574 | 47 | 19 | 0 | 2 | 48 | 60 | 106 | 1.73e-12 | 60.8 |
| MsG0280006752.01.T01 | MTR_5g085100 | 59.574 | 47 | 19 | 0 | 2 | 48 | 55 | 101 | 4.38e-12 | 59.7 |
| MsG0280006752.01.T01 | MTR_2g078680 | 92.857 | 28 | 2 | 0 | 2 | 29 | 40 | 67 | 5.25e-11 | 56.2 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MsG0280006752.01.T01 | AT1G28360 | 62.295 | 61 | 18 | 1 | 2 | 62 | 21 | 76 | 2.26e-16 | 70.1 |
| MsG0280006752.01.T01 | AT1G28370 | 51.613 | 62 | 22 | 1 | 2 | 63 | 29 | 82 | 4.94e-13 | 60.8 |
| MsG0280006752.01.T01 | AT1G28370 | 92.857 | 28 | 2 | 0 | 2 | 29 | 67 | 94 | 1.08e-12 | 60.5 |
| MsG0280006752.01.T01 | AT5G44210 | 92.857 | 28 | 2 | 0 | 2 | 29 | 42 | 69 | 2.81e-11 | 56.6 |
| MsG0280006752.01.T01 | AT3G15210 | 92.857 | 28 | 2 | 0 | 2 | 29 | 34 | 61 | 3.34e-11 | 57.0 |
Find 28 sgRNAs with CRISPR-Local
Find 27 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| GTAAGCAGAGCAGCTTCTTC+TGG | 0.305471 | 2:-5829863 | None:intergenic |
| GTCCTCCGGGCGATCGGCGC+CGG | 0.416232 | 2:-5829937 | None:intergenic |
| TTCCACCGGCGCCGATCGCC+CGG | 0.419127 | 2:+5829932 | MsG0280006752.01.T01:CDS |
| CATGGAAGAAGACACGAGTT+TGG | 0.440857 | 2:+5829822 | MsG0280006752.01.T01:CDS |
| ATCGCCCGGAGGACTTTGCT+TGG | 0.442623 | 2:+5829946 | MsG0280006752.01.T01:CDS |
| CAAACTCGTGTCTTCTTCCA+TGG | 0.475352 | 2:-5829821 | None:intergenic |
| GATCGGCGCCGGTGGAAAGT+TGG | 0.494900 | 2:-5829926 | None:intergenic |
| GAAGAAGACACGAGTTTGGT+TGG | 0.503288 | 2:+5829826 | MsG0280006752.01.T01:CDS |
| AGCAAAGTCCTCCGGGCGAT+CGG | 0.504347 | 2:-5829943 | None:intergenic |
| TAGATCCAAGCAAAGTCCTC+CGG | 0.504773 | 2:-5829951 | None:intergenic |
| CTCCGGGCGATCGGCGCCGG+TGG | 0.508879 | 2:-5829934 | None:intergenic |
| AAGAAGACACGAGTTTGGTT+GGG | 0.528467 | 2:+5829827 | MsG0280006752.01.T01:CDS |
| CCGCTGAGATTCGTGATCCA+TGG | 0.530373 | 2:+5829804 | MsG0280006752.01.T01:CDS |
| CTCCTTCTTCTGATCTCCGA+TGG | 0.535940 | 2:+5829978 | MsG0280006752.01.T01:CDS |
| CGTGGCCGTGTGCTCACCTG+AGG | 0.544783 | 2:+5830016 | MsG0280006752.01.T01:CDS |
| AGATCCAAGCAAAGTCCTCC+GGG | 0.548638 | 2:-5829950 | None:intergenic |
| CTCAGCGGCGTAGCGTCCCA+TGG | 0.550833 | 2:-5829789 | None:intergenic |
| GTGAGCACACGGCCACGTGA+AGG | 0.561706 | 2:-5830010 | None:intergenic |
| GGCCATCGGAGATCAGAAGA+AGG | 0.567898 | 2:-5829980 | None:intergenic |
| TGAGCACACGGCCACGTGAA+GGG | 0.577758 | 2:-5830009 | None:intergenic |
| CGGCCACGTGAAGGGACAGA+AGG | 0.591005 | 2:-5830001 | None:intergenic |
| TGGCCTTCTGTCCCTTCACG+TGG | 0.605659 | 2:+5829998 | MsG0280006752.01.T01:CDS |
| GGCGCTGCTCGTTCTCTCCG+AGG | 0.622972 | 2:+5829889 | MsG0280006752.01.T01:CDS |
| GTGAAGGGACAGAAGGCCAT+CGG | 0.628720 | 2:-5829994 | None:intergenic |
| AGAAACCTCAGGTGAGCACA+CGG | 0.636816 | 2:-5830021 | None:intergenic |
| CACCGGCGCCGATCGCCCGG+AGG | 0.673714 | 2:+5829935 | MsG0280006752.01.T01:CDS |
| AGAAGCTGCTCTGCTTACGA+CGG | 0.675482 | 2:+5829868 | MsG0280006752.01.T01:CDS |
| CCATGGATCACGAATCTCAG+CGG | 0.721133 | 2:-5829804 | None:intergenic |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|---|---|---|---|---|
| AAGAAGACACGAGTTTGGTT+GGG | + | Chr2:5829827-5829846 | MsG0280006752.01.T01:CDS | 40.0% | |
| AGCAAAAACCAACTTTCCAC+CGG | + | Chr2:5829918-5829937 | MsG0280006752.01.T01:CDS | 40.0% | |
| !! | TGGTTTTTGCTTTTGCTCCT+CGG | - | Chr2:5829909-5829928 | None:intergenic | 40.0% |
| CAAACTCGTGTCTTCTTCCA+TGG | - | Chr2:5829824-5829843 | None:intergenic | 45.0% | |
| CATGGAAGAAGACACGAGTT+TGG | + | Chr2:5829822-5829841 | MsG0280006752.01.T01:CDS | 45.0% | |
| GAAGAAGACACGAGTTTGGT+TGG | + | Chr2:5829826-5829845 | MsG0280006752.01.T01:CDS | 45.0% | |
| TAGATCCAAGCAAAGTCCTC+CGG | - | Chr2:5829954-5829973 | None:intergenic | 45.0% | |
| AGAAGCTGCTCTGCTTACGA+CGG | + | Chr2:5829868-5829887 | MsG0280006752.01.T01:CDS | 50.0% | |
| AGATCCAAGCAAAGTCCTCC+GGG | - | Chr2:5829953-5829972 | None:intergenic | 50.0% | |
| CCATGGATCACGAATCTCAG+CGG | - | Chr2:5829807-5829826 | None:intergenic | 50.0% | |
| CTCCTTCTTCTGATCTCCGA+TGG | + | Chr2:5829978-5829997 | MsG0280006752.01.T01:CDS | 50.0% | |
| GTAAGCAGAGCAGCTTCTTC+TGG | - | Chr2:5829866-5829885 | None:intergenic | 50.0% | |
| CCGCTGAGATTCGTGATCCA+TGG | + | Chr2:5829804-5829823 | MsG0280006752.01.T01:CDS | 55.0% | |
| GGCCATCGGAGATCAGAAGA+AGG | - | Chr2:5829983-5830002 | None:intergenic | 55.0% | |
| GTGAAGGGACAGAAGGCCAT+CGG | - | Chr2:5829997-5830016 | None:intergenic | 55.0% | |
| AGCAAAGTCCTCCGGGCGAT+CGG | - | Chr2:5829946-5829965 | None:intergenic | 60.0% | |
| ATCGCCCGGAGGACTTTGCT+TGG | + | Chr2:5829946-5829965 | MsG0280006752.01.T01:CDS | 60.0% | |
| TGAGCACACGGCCACGTGAA+GGG | - | Chr2:5830012-5830031 | None:intergenic | 60.0% | |
| TGGCCTTCTGTCCCTTCACG+TGG | + | Chr2:5829998-5830017 | MsG0280006752.01.T01:CDS | 60.0% | |
| CGGCCACGTGAAGGGACAGA+AGG | - | Chr2:5830004-5830023 | None:intergenic | 65.0% | |
| GTGAGCACACGGCCACGTGA+AGG | - | Chr2:5830013-5830032 | None:intergenic | 65.0% | |
| !! | GATCGGCGCCGGTGGAAAGT+TGG | - | Chr2:5829929-5829948 | None:intergenic | 65.0% |
| GGCGCTGCTCGTTCTCTCCG+AGG | + | Chr2:5829889-5829908 | MsG0280006752.01.T01:CDS | 70.0% | |
| !! | TTCCACCGGCGCCGATCGCC+CGG | + | Chr2:5829932-5829951 | MsG0280006752.01.T01:CDS | 75.0% |
| ! | GTCCTCCGGGCGATCGGCGC+CGG | - | Chr2:5829940-5829959 | None:intergenic | 80.0% |
| !! | CTCCGGGCGATCGGCGCCGG+TGG | - | Chr2:5829937-5829956 | None:intergenic | 85.0% |
| !!! | CACCGGCGCCGATCGCCCGG+AGG | + | Chr2:5829935-5829954 | MsG0280006752.01.T01:CDS | 85.0% |
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| Chr2 | gene | 5829791 | 5830036 | 5829791 | ID=MsG0280006752.01;Name=MsG0280006752.01 |
| Chr2 | mRNA | 5829791 | 5830036 | 5829791 | ID=MsG0280006752.01.T01;Parent=MsG0280006752.01;Name=MsG0280006752.01.T01;_AED=0.26;_eAED=0.26;_QI=0|-1|0|1|-1|1|1|0|81 |
| Chr2 | exon | 5829791 | 5830036 | 5829791 | ID=MsG0280006752.01.T01:exon:2363;Parent=MsG0280006752.01.T01 |
| Chr2 | CDS | 5829791 | 5830036 | 5829791 | ID=MsG0280006752.01.T01:cds;Parent=MsG0280006752.01.T01 |
| Gene Sequence |
| Protein sequence |
