AlfalfaGEDB | MsG0280006944.01

M. sativa cultivar ZhongmuNo.1 / MsG0280006944.01

KEGG: -
GO: GO:0003676
PFAM: PF13456

Nr:

Query id	Subject id	identity %	alignment length	mis matches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MsG0280006944.01.T01	XP_039686983.1	81.731	104	19	0	1	104	1	104	1.11E-55	179

Swissprot:

Query id	Subject id	identity %	alignment length	mis matches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score

Trembl:

Query id	Subject id	identity %	alignment length	mis matches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MsG0280006944.01.T01	A2Q4N3	69.608	102	31	0	3	104	91	192	2.17e-46	157

TFs/TRs:

Gene ID	Type	Classification

Protein Kinases:

Gene ID	Type	Classification

Network:

Co-expression Network

Gene1	Gene2	correlation coefficient	p_value	FDR

PPI

Gene1	Gene2	Type
MsG0280006944.01	MsG0280009322.01	PPI
MsG0280006944.01	MsG0380013578.01	PPI
MsG0280006944.01	MsG0280010449.01	PPI
MsG0280006944.01	MsG0780041116.01	PPI
MsG0280006944.01	MsG0780040274.01	PPI
MsG0280006944.01	MsG0780041116.01	PPI

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MsG0280006944.01.T01	MTR_2g017550	85.526	76	11	0	2	77	85	160	3.51e-44	141
MsG0280006944.01.T01	MTR_6g089560	56.863	102	44	0	3	104	94	195	2.42e-41	136
MsG0280006944.01.T01	MTR_8g099580	53.922	102	47	0	3	104	184	285	1.69e-37	128
MsG0280006944.01.T01	MTR_7g013520	57.576	99	42	0	4	102	49	147	1.78e-37	124
MsG0280006944.01.T01	MTR_1g017840	57.732	97	41	0	7	103	60	156	4.33e-37	123
MsG0280006944.01.T01	MTR_5g037920	54.082	98	44	1	7	104	80	176	1.60e-31	110
MsG0280006944.01.T01	MTR_5g049680	50.505	99	48	1	3	100	9	107	2.86e-31	106
MsG0280006944.01.T01	MTR_6g016265	46.535	101	54	0	4	104	18	118	1.25e-30	105
MsG0280006944.01.T01	MTR_6g453270	44.660	103	57	0	1	103	276	378	2.48e-27	103
MsG0280006944.01.T01	MTR_8g098925	47.059	102	40	1	3	104	57	144	3.79e-27	97.8
MsG0280006944.01.T01	MTR_3g116210	41.176	102	60	0	3	104	75	176	7.98e-27	97.8
MsG0280006944.01.T01	MTR_4g029360	48.039	102	53	0	3	104	105	206	9.65e-27	98.6
MsG0280006944.01.T01	MTR_1g069475	42.157	102	59	0	3	104	142	243	1.12e-25	96.3
MsG0280006944.01.T01	MTR_1g074090	48.315	89	46	0	3	91	59	147	3.20e-25	92.8
MsG0280006944.01.T01	MTR_1g081930	42.574	101	58	0	4	104	17	117	5.34e-25	91.7
MsG0280006944.01.T01	MTR_0093s0070	56.579	76	33	0	29	104	127	202	5.97e-25	93.6
MsG0280006944.01.T01	MTR_6g046670	56.579	76	33	0	3	78	101	176	1.19e-24	92.4
MsG0280006944.01.T01	MTR_1g022215	55.072	69	31	0	13	81	2	70	2.15e-24	89.0
MsG0280006944.01.T01	MTR_0015s0120	51.685	89	43	0	10	98	278	366	2.95e-24	94.7
MsG0280006944.01.T01	MTR_1g054905	61.194	67	26	0	2	68	78	144	2.96e-24	90.5
MsG0280006944.01.T01	MTR_6g033225	53.012	83	39	0	10	92	55	137	6.48e-24	89.0
MsG0280006944.01.T01	MTR_6g045427	46.535	101	26	1	1	101	149	221	1.90e-23	90.5
MsG0280006944.01.T01	MTR_1g034430	42.157	102	59	0	3	104	73	174	4.56e-23	88.2
MsG0280006944.01.T01	MTR_5g036320	43.820	89	50	0	2	90	56	144	1.03e-22	86.3
MsG0280006944.01.T01	MTR_7g063280	45.161	93	48	1	12	104	62	151	1.38e-22	86.7
MsG0280006944.01.T01	MTR_5g034450	52.000	75	36	0	10	84	34	108	1.39e-22	84.7
MsG0280006944.01.T01	MTR_1g088650	44.118	102	57	0	3	104	64	165	3.18e-22	85.9
MsG0280006944.01.T01	MTR_5g083200	35.294	102	66	0	3	104	76	177	3.23e-22	86.3
MsG0280006944.01.T01	MTR_0002s1220	35.294	102	66	0	3	104	76	177	3.23e-22	86.3
MsG0280006944.01.T01	MTR_7g078610	57.812	64	27	0	4	67	30	93	3.36e-22	83.2
MsG0280006944.01.T01	MTR_1g018300	47.525	101	47	1	4	104	42	136	8.34e-22	86.3
MsG0280006944.01.T01	MTR_2g027020	40.777	103	57	1	2	104	5	103	8.65e-22	84.7
MsG0280006944.01.T01	MTR_3g111390	34.314	102	67	0	3	104	76	177	1.76e-21	84.3
MsG0280006944.01.T01	MTR_1g070820	34.314	102	67	0	3	104	76	177	1.76e-21	84.3
MsG0280006944.01.T01	MTR_2g047100	44.000	100	38	2	5	104	1	82	2.30e-21	81.3
MsG0280006944.01.T01	MTR_8g036780	43.373	83	47	0	1	83	10	92	2.36e-21	81.6
MsG0280006944.01.T01	MTR_8g031800	41.414	99	58	0	2	100	8	106	2.48e-21	81.6
MsG0280006944.01.T01	MTR_8g038340	48.750	80	41	0	3	82	167	246	2.79e-21	84.7
MsG0280006944.01.T01	MTR_1g050488	44.444	81	45	0	10	90	83	163	1.07e-20	81.6
MsG0280006944.01.T01	MTR_1g045340	35.714	98	63	0	7	104	66	163	5.17e-20	80.1
MsG0280006944.01.T01	MTR_5g085210	39.216	102	62	0	3	104	97	198	2.62e-19	79.3
MsG0280006944.01.T01	MTR_2g059470	46.053	76	41	0	29	104	4	79	4.85e-19	75.1
MsG0280006944.01.T01	MTR_1g099430	39.216	102	52	2	2	93	102	203	1.36e-18	77.0
MsG0280006944.01.T01	MTR_8g079540	42.683	82	47	0	3	84	26	107	4.76e-18	73.6
MsG0280006944.01.T01	MTR_8g022750	36.275	102	48	1	3	104	183	267	9.15e-18	76.3
MsG0280006944.01.T01	MTR_4g094272	47.143	70	36	1	3	72	72	140	9.31e-17	70.9
MsG0280006944.01.T01	MTR_1g109000	39.506	81	49	0	4	84	5	85	3.63e-16	67.8
MsG0280006944.01.T01	MTR_2g055490	56.140	57	25	0	30	86	12	68	7.56e-16	67.8
MsG0280006944.01.T01	MTR_6g034915	28.682	129	62	1	1	99	1	129	7.83e-16	69.3
MsG0280006944.01.T01	MTR_4g008270	41.250	80	47	0	25	104	10	89	9.87e-16	67.0
MsG0280006944.01.T01	MTR_8g059135	39.474	76	46	0	29	104	32	107	1.61e-15	67.4
MsG0280006944.01.T01	MTR_4g046027	46.269	67	36	0	1	67	103	169	8.84e-15	66.2
MsG0280006944.01.T01	MTR_8g028785	33.010	103	45	1	2	104	259	337	1.43e-14	68.2
MsG0280006944.01.T01	MTR_1g062840	53.846	52	24	0	53	104	8	59	1.59e-14	63.5
MsG0280006944.01.T01	MTR_3g102000	58.696	46	19	0	3	48	181	226	1.73e-14	66.6
MsG0280006944.01.T01	MTR_7g053400	40.625	64	38	0	21	84	171	234	2.30e-14	66.6
MsG0280006944.01.T01	MTR_5g075610	43.836	73	39	1	32	104	2	72	3.40e-14	63.2
MsG0280006944.01.T01	MTR_8g031080	44.444	63	35	0	2	64	266	328	3.74e-14	67.0
MsG0280006944.01.T01	MTR_6g016355	37.500	72	45	0	13	84	104	175	4.34e-14	64.7
MsG0280006944.01.T01	MTR_8g088220	37.500	72	45	0	33	104	2	73	8.21e-14	61.6
MsG0280006944.01.T01	MTR_8g069135	60.000	45	18	0	3	47	167	211	1.36e-13	65.1
MsG0280006944.01.T01	MTR_1g074240	50.000	56	28	0	29	84	4	59	3.48e-13	59.7
MsG0280006944.01.T01	MTR_6g016135	47.541	61	32	0	28	88	382	442	7.78e-13	63.2
MsG0280006944.01.T01	MTR_5g068115	43.103	58	33	0	1	58	10	67	8.79e-13	58.5
MsG0280006944.01.T01	MTR_1g087480	50.909	55	27	0	3	57	166	220	1.29e-12	61.6
MsG0280006944.01.T01	MTR_3g462490	42.188	64	37	0	2	65	78	141	2.41e-12	61.6
MsG0280006944.01.T01	MTR_5g069700	48.148	54	28	0	51	104	7	60	2.81e-12	57.8
MsG0280006944.01.T01	MTR_6g023630	46.552	58	30	1	38	94	2	59	4.23e-12	57.4
MsG0280006944.01.T01	MTR_1g045560	37.500	64	40	0	31	94	1	64	6.46e-12	57.0
MsG0280006944.01.T01	MTR_4g094370	42.424	66	38	0	2	67	12	77	7.07e-12	56.6
MsG0280006944.01.T01	MTR_3g060800	57.143	42	18	0	4	45	20	61	2.30e-11	54.7
MsG0280006944.01.T01	MTR_8g045330	36.986	73	45	1	4	76	76	147	2.59e-11	57.0
MsG0280006944.01.T01	MTR_4g073820	47.273	55	27	2	31	85	1	53	3.22e-11	54.7
MsG0280006944.01.T01	MTR_4g035890	37.288	59	37	0	1	59	90	148	6.67e-11	55.8
MsG0280006944.01.T01	MTR_3g064060	50.000	42	21	0	4	45	16	57	6.86e-11	53.5
MsG0280006944.01.T01	MTR_7g072030	49.020	51	26	0	48	98	72	122	6.93e-11	55.1
MsG0280006944.01.T01	MTR_1964s0010	47.826	46	24	0	3	48	47	92	7.62e-11	54.7

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score

Find 24 sgRNAs with CRISPR-Local

Find 25 sgRNAs with CRISPR-GE

CRISPR-Local

CRISPR-Local
sgRNA_sequence	on_target_score	Position	Region
CATAGCGATGTTACAGAATT+TGG	0.128194	2:+8361302	MsG0280006944.01.T01:CDS
CACGTATTGGCTTCTGCCTT+CGG	0.301106	2:+8361102	MsG0280006944.01.T01:CDS
ATAGCAGTTGGTGAAGCTTT+GGG	0.337244	2:+8361182	MsG0280006944.01.T01:CDS
CATAGCAGTTGGTGAAGCTT+TGG	0.363609	2:+8361181	MsG0280006944.01.T01:CDS
CTTAGCCAACACGAAAGTTC+TGG	0.370580	2:-8361132	None:intergenic
CAATTTCAGCGGACACGTAT+TGG	0.375129	2:+8361089	MsG0280006944.01.T01:CDS
CAGTGGATGCCACCGAGTCT+TGG	0.411625	2:+8361029	MsG0280006944.01.T01:CDS
AGTGGATGCCACCGAGTCTT+GGG	0.420803	2:+8361030	MsG0280006944.01.T01:CDS
ACGTATTGGCTTCTGCCTTC+GGG	0.457984	2:+8361103	MsG0280006944.01.T01:CDS
CAAAGCTTCACCAACTGCTA+TGG	0.468752	2:-8361180	None:intergenic
CAAATTCTGTAACATCGCTA+TGG	0.489523	2:-8361301	None:intergenic
AAATTCTGTAACATCGCTAT+GGG	0.492543	2:-8361300	None:intergenic
AGTGCATGTCTTGCAGCCAC+TGG	0.492804	2:-8361220	None:intergenic
CCGCTGAAATTGATTCGAAA+AGG	0.498588	2:-8361078	None:intergenic
AAAGCTTCACCAACTGCTAT+GGG	0.530540	2:-8361179	None:intergenic
CATCTTTATCCCATAGCAGT+TGG	0.545036	2:+8361170	MsG0280006944.01.T01:CDS
GTCTGTTTCATGCTCTCCAG+TGG	0.552017	2:+8361204	MsG0280006944.01.T01:CDS
CATTTGTATCTCCCAAGACT+CGG	0.575573	2:-8361041	None:intergenic
CATGTGCTGTCATGCATCAG+TGG	0.603813	2:+8361012	None:intergenic
TGATTCCAGAACTTTCGTGT+TGG	0.611980	2:+8361127	MsG0280006944.01.T01:CDS
ATACAAATGTAATATTGATG+CGG	0.656759	2:+8361055	MsG0280006944.01.T01:CDS
TTGTATCTCCCAAGACTCGG+TGG	0.672931	2:-8361038	None:intergenic
AAGTTCTGGAATCATCCCGA+AGG	0.681970	2:-8361118	None:intergenic
AATTCTGTAACATCGCTATG+GGG	0.723550	2:-8361299	None:intergenic

CRISPR-GE

badsite warning	sgRNA_sequence	Strand	Position	Region	GC_content
!!	ATACAAATGTAATATTGATG+CGG	+	Chr2:8361055-8361074	MsG0280006944.01.T01:CDS	20.0%
	AAATTCTGTAACATCGCTAT+GGG	-	Chr2:8361303-8361322	None:intergenic	30.0%
!	TGATTTTGAGCTTGATTCAA+AGG	+	Chr2:8361253-8361272	MsG0280006944.01.T01:CDS	30.0%
	AATTCTGTAACATCGCTATG+GGG	-	Chr2:8361302-8361321	None:intergenic	35.0%
	CAAATTCTGTAACATCGCTA+TGG	-	Chr2:8361304-8361323	None:intergenic	35.0%
	CATAGCGATGTTACAGAATT+TGG	+	Chr2:8361302-8361321	MsG0280006944.01.T01:CDS	35.0%
!	CCTTTTCGAATCAATTTCAG+CGG	+	Chr2:8361078-8361097	MsG0280006944.01.T01:CDS	35.0%
	AAAGCTTCACCAACTGCTAT+GGG	-	Chr2:8361182-8361201	None:intergenic	40.0%
	CATCTTTATCCCATAGCAGT+TGG	+	Chr2:8361170-8361189	MsG0280006944.01.T01:CDS	40.0%
	CATTTGTATCTCCCAAGACT+CGG	-	Chr2:8361044-8361063	None:intergenic	40.0%
	TGATTCCAGAACTTTCGTGT+TGG	+	Chr2:8361127-8361146	MsG0280006944.01.T01:CDS	40.0%
!!	ATAGCAGTTGGTGAAGCTTT+GGG	+	Chr2:8361182-8361201	MsG0280006944.01.T01:CDS	40.0%
!!	CCGCTGAAATTGATTCGAAA+AGG	-	Chr2:8361081-8361100	None:intergenic	40.0%
	CAAAGCTTCACCAACTGCTA+TGG	-	Chr2:8361183-8361202	None:intergenic	45.0%
	CAATTTCAGCGGACACGTAT+TGG	+	Chr2:8361089-8361108	MsG0280006944.01.T01:CDS	45.0%
	CTTAGCCAACACGAAAGTTC+TGG	-	Chr2:8361135-8361154	None:intergenic	45.0%
!!	AAGTTCTGGAATCATCCCGA+AGG	-	Chr2:8361121-8361140	None:intergenic	45.0%
!!	CATAGCAGTTGGTGAAGCTT+TGG	+	Chr2:8361181-8361200	MsG0280006944.01.T01:CDS	45.0%
	GTCTGTTTCATGCTCTCCAG+TGG	+	Chr2:8361204-8361223	MsG0280006944.01.T01:CDS	50.0%
	TTGTATCTCCCAAGACTCGG+TGG	-	Chr2:8361041-8361060	None:intergenic	50.0%
!!	ACGTATTGGCTTCTGCCTTC+GGG	+	Chr2:8361103-8361122	MsG0280006944.01.T01:CDS	50.0%
!!	CACGTATTGGCTTCTGCCTT+CGG	+	Chr2:8361102-8361121	MsG0280006944.01.T01:CDS	50.0%
	AGTGCATGTCTTGCAGCCAC+TGG	-	Chr2:8361223-8361242	None:intergenic	55.0%
	AGTGGATGCCACCGAGTCTT+GGG	+	Chr2:8361030-8361049	MsG0280006944.01.T01:CDS	55.0%
	CAGTGGATGCCACCGAGTCT+TGG	+	Chr2:8361029-8361048	MsG0280006944.01.T01:CDS	60.0%

Chromosome	Type	Strat	End	Strand	Name
Chr2	gene	8361023	8361337	8361023	ID=MsG0280006944.01;Name=MsG0280006944.01
Chr2	mRNA	8361023	8361337	8361023	ID=MsG0280006944.01.T01;Parent=MsG0280006944.01;Name=MsG0280006944.01.T01;_AED=0.50;_eAED=0.50;_QI=0\|-1\|0\|1\|-1\|1\|1\|0\|104
Chr2	exon	8361023	8361337	8361023	ID=MsG0280006944.01.T01:exon:19393;Parent=MsG0280006944.01.T01
Chr2	CDS	8361023	8361337	8361023	ID=MsG0280006944.01.T01:cds;Parent=MsG0280006944.01.T01

Gene Sequence	>MsG0280006944.01.T01 ATGCATCAGTGGATGCCACCGAGTCTTGGGAGATACAAATGTAATATTGATGCGGCCTTTTCGAATCAATTTCAGCGGACACGTATTGGCTTCTGCCTTCGGGATGATTCCAGAACTTTCGTGTTGGCTAAGACGTTACAGTTCAATCATCTTTATCCCATAGCAGTTGGTGAAGCTTTGGGTCTGTTTCATGCTCTCCAGTGGCTGCAAGACATGCACTTTGATTATATTGATTTTGAGCTTGATTCAAAGGTTACACGAGATGCTTTCTATTCTCCCCATAGCGATGTTACAGAATTTGGAAGTATCTTGTAG
Protein sequence	>MsG0280006944.01.T01 MHQWMPPSLGRYKCNIDAAFSNQFQRTRIGFCLRDDSRTFVLAKTLQFNHLYPIAVGEALGLFHALQWLQDMHFDYIDFELDSKVTRDAFYSPHSDVTEFGSIL*