Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MsG0280007061.01.T01 | XP_003593959.1 | 97.143 | 210 | 5 | 1 | 1 | 209 | 3 | 212 | 1.61E-142 | 406 |
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MsG0280007061.01.T01 | Q9ZRA4 | 49.524 | 210 | 95 | 5 | 4 | 208 | 5 | 208 | 1.31E-60 | 191 |
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MsG0280007061.01.T01 | G7IPZ2 | 97.143 | 210 | 5 | 1 | 1 | 209 | 3 | 212 | 7.69e-143 | 406 |
| Gene ID | Type | Classification |
|---|
| Gene ID | Type | Classification |
|---|
Co-expression Network
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|---|---|---|---|
| MsG0080048931.01 | MsG0280007061.01 | 0.999626 | 0.000000e+00 | 0.000000e+00 |
| MsG0280007061.01 | MsG0580028527.01 | 0.800612 | 1.328302e-48 | 6.212244e-46 |
PPI
| Gene1 | Gene2 | Type |
|---|
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MsG0280007061.01.T01 | MTR_2g019780 | 97.143 | 210 | 5 | 1 | 1 | 209 | 3 | 212 | 1.95e-146 | 406 |
| MsG0280007061.01.T01 | MTR_3g073170 | 84.211 | 209 | 32 | 1 | 1 | 209 | 3 | 210 | 2.28e-115 | 327 |
| MsG0280007061.01.T01 | MTR_4g010340 | 64.623 | 212 | 65 | 5 | 1 | 209 | 1 | 205 | 1.08e-84 | 249 |
| MsG0280007061.01.T01 | MTR_8g020650 | 58.333 | 192 | 76 | 2 | 21 | 209 | 19 | 209 | 1.03e-76 | 229 |
| MsG0280007061.01.T01 | MTR_8g020630 | 56.250 | 208 | 84 | 4 | 5 | 209 | 6 | 209 | 1.71e-76 | 229 |
| MsG0280007061.01.T01 | MTR_8g020760 | 58.571 | 210 | 81 | 4 | 3 | 209 | 5 | 211 | 4.01e-74 | 223 |
| MsG0280007061.01.T01 | MTR_8g020670 | 54.106 | 207 | 89 | 3 | 6 | 209 | 6 | 209 | 3.57e-73 | 220 |
| MsG0280007061.01.T01 | MTR_2g044040 | 53.774 | 212 | 92 | 3 | 1 | 209 | 1 | 209 | 3.31e-72 | 218 |
| MsG0280007061.01.T01 | MTR_8g020750 | 54.930 | 213 | 89 | 4 | 1 | 209 | 3 | 212 | 1.03e-70 | 214 |
| MsG0280007061.01.T01 | MTR_7g011340 | 57.447 | 188 | 76 | 2 | 25 | 209 | 23 | 209 | 1.33e-69 | 211 |
| MsG0280007061.01.T01 | MTR_0284s0010 | 53.846 | 208 | 89 | 4 | 5 | 209 | 6 | 209 | 1.82e-69 | 211 |
| MsG0280007061.01.T01 | MTR_2g045100 | 52.885 | 208 | 88 | 3 | 5 | 209 | 9 | 209 | 2.10e-69 | 211 |
| MsG0280007061.01.T01 | MTR_3g019710 | 52.358 | 212 | 95 | 3 | 1 | 209 | 1 | 209 | 3.17e-69 | 210 |
| MsG0280007061.01.T01 | MTR_8g020763 | 61.582 | 177 | 64 | 2 | 37 | 209 | 14 | 190 | 4.21e-68 | 207 |
| MsG0280007061.01.T01 | MTR_2g036120 | 51.442 | 208 | 96 | 3 | 4 | 208 | 3 | 208 | 1.94e-67 | 206 |
| MsG0280007061.01.T01 | MTR_3g019680 | 52.451 | 204 | 91 | 3 | 9 | 209 | 8 | 208 | 5.42e-67 | 205 |
| MsG0280007061.01.T01 | MTR_3g019650 | 51.456 | 206 | 95 | 3 | 8 | 209 | 4 | 208 | 1.76e-66 | 203 |
| MsG0280007061.01.T01 | MTR_5g031460 | 50.943 | 212 | 98 | 3 | 1 | 209 | 1 | 209 | 5.90e-66 | 202 |
| MsG0280007061.01.T01 | MTR_8g020770 | 49.533 | 214 | 99 | 5 | 1 | 209 | 3 | 212 | 1.98e-63 | 196 |
| MsG0280007061.01.T01 | MTR_8g020590 | 46.078 | 204 | 104 | 4 | 11 | 209 | 6 | 208 | 6.44e-59 | 184 |
| MsG0280007061.01.T01 | MTR_8g020610 | 46.602 | 206 | 104 | 5 | 9 | 209 | 3 | 207 | 3.26e-56 | 177 |
| MsG0280007061.01.T01 | MTR_2g041550 | 41.981 | 212 | 111 | 6 | 4 | 209 | 5 | 210 | 1.78e-50 | 163 |
| MsG0280007061.01.T01 | MTR_2g041570 | 41.121 | 214 | 117 | 5 | 1 | 209 | 1 | 210 | 7.54e-50 | 161 |
| MsG0280007061.01.T01 | MTR_2g041580 | 41.121 | 214 | 117 | 5 | 1 | 209 | 1 | 210 | 1.84e-49 | 160 |
| MsG0280007061.01.T01 | MTR_3g019810 | 43.961 | 207 | 102 | 5 | 1 | 203 | 107 | 303 | 6.02e-46 | 154 |
| MsG0280007061.01.T01 | MTR_4g027415 | 57.025 | 121 | 48 | 2 | 25 | 142 | 23 | 142 | 1.99e-37 | 127 |
| MsG0280007061.01.T01 | MTR_1g079490 | 37.559 | 213 | 123 | 4 | 3 | 205 | 5 | 217 | 5.24e-36 | 126 |
| MsG0280007061.01.T01 | MTR_1g085140 | 33.511 | 188 | 117 | 5 | 25 | 205 | 29 | 215 | 1.46e-27 | 104 |
| MsG0280007061.01.T01 | MTR_7g029500 | 88.333 | 60 | 3 | 1 | 150 | 209 | 38 | 93 | 3.42e-27 | 99.8 |
| MsG0280007061.01.T01 | MTR_6g005360 | 33.333 | 213 | 130 | 6 | 3 | 205 | 5 | 215 | 7.13e-27 | 102 |
| MsG0280007061.01.T01 | MTR_6g005340 | 33.491 | 212 | 129 | 6 | 4 | 205 | 6 | 215 | 9.00e-27 | 102 |
| MsG0280007061.01.T01 | MTR_1g060120 | 31.455 | 213 | 135 | 5 | 4 | 205 | 9 | 221 | 9.90e-27 | 102 |
| MsG0280007061.01.T01 | MTR_6g005330 | 33.962 | 212 | 130 | 5 | 3 | 205 | 5 | 215 | 1.63e-26 | 101 |
| MsG0280007061.01.T01 | MTR_6g005310 | 33.491 | 212 | 129 | 6 | 4 | 205 | 6 | 215 | 3.43e-26 | 100 |
| MsG0280007061.01.T01 | MTR_1g085500 | 30.952 | 210 | 137 | 5 | 2 | 204 | 5 | 213 | 3.41e-25 | 98.2 |
| MsG0280007061.01.T01 | MTR_2g030855 | 34.536 | 194 | 114 | 5 | 21 | 205 | 23 | 212 | 3.80e-25 | 97.8 |
| MsG0280007061.01.T01 | MTR_2g072560 | 46.610 | 118 | 55 | 4 | 1 | 115 | 3 | 115 | 5.42e-25 | 95.5 |
| MsG0280007061.01.T01 | MTR_1g112200 | 32.258 | 186 | 113 | 7 | 26 | 205 | 29 | 207 | 6.44e-25 | 97.4 |
| MsG0280007061.01.T01 | MTR_2g031270 | 33.505 | 194 | 116 | 5 | 21 | 205 | 24 | 213 | 6.75e-25 | 97.4 |
| MsG0280007061.01.T01 | MTR_8g007300 | 34.320 | 169 | 94 | 3 | 40 | 204 | 47 | 202 | 7.40e-25 | 97.4 |
| MsG0280007061.01.T01 | MTR_4g017040 | 32.864 | 213 | 130 | 6 | 3 | 204 | 5 | 215 | 1.73e-24 | 96.7 |
| MsG0280007061.01.T01 | MTR_6g005380 | 38.710 | 155 | 86 | 4 | 22 | 168 | 24 | 177 | 2.55e-24 | 95.9 |
| MsG0280007061.01.T01 | MTR_4g017050 | 32.864 | 213 | 130 | 6 | 3 | 204 | 5 | 215 | 2.78e-24 | 95.9 |
| MsG0280007061.01.T01 | MTR_5g046410 | 32.864 | 213 | 130 | 6 | 3 | 204 | 5 | 215 | 3.39e-24 | 95.9 |
| MsG0280007061.01.T01 | MTR_5g046430 | 32.864 | 213 | 130 | 6 | 3 | 204 | 5 | 215 | 3.39e-24 | 95.9 |
| MsG0280007061.01.T01 | MTR_4g017030 | 32.864 | 213 | 130 | 6 | 3 | 204 | 5 | 215 | 3.39e-24 | 95.9 |
| MsG0280007061.01.T01 | MTR_2g019250 | 32.394 | 213 | 131 | 6 | 3 | 204 | 5 | 215 | 1.27e-23 | 94.4 |
| MsG0280007061.01.T01 | MTR_1g023770 | 32.474 | 194 | 115 | 5 | 21 | 205 | 23 | 209 | 3.74e-23 | 92.8 |
| MsG0280007061.01.T01 | MTR_2g096110 | 37.255 | 153 | 72 | 4 | 45 | 194 | 7 | 138 | 5.66e-23 | 90.5 |
| MsG0280007061.01.T01 | MTR_5g036570 | 30.526 | 190 | 123 | 6 | 26 | 207 | 33 | 221 | 7.61e-23 | 92.0 |
| MsG0280007061.01.T01 | MTR_7g111240 | 32.447 | 188 | 116 | 5 | 25 | 205 | 25 | 208 | 9.58e-23 | 91.7 |
| MsG0280007061.01.T01 | MTR_7g111240 | 31.915 | 188 | 120 | 5 | 25 | 205 | 25 | 211 | 1.01e-21 | 89.0 |
| MsG0280007061.01.T01 | MTR_2g086630 | 35.404 | 161 | 98 | 4 | 50 | 205 | 45 | 204 | 1.03e-21 | 89.0 |
| MsG0280007061.01.T01 | MTR_3g019610 | 48.544 | 103 | 47 | 3 | 8 | 105 | 4 | 105 | 1.22e-21 | 85.9 |
| MsG0280007061.01.T01 | MTR_4g052780 | 36.691 | 139 | 86 | 2 | 69 | 205 | 67 | 205 | 1.75e-21 | 88.2 |
| MsG0280007061.01.T01 | MTR_2g086620 | 34.783 | 161 | 99 | 4 | 50 | 205 | 45 | 204 | 4.86e-21 | 87.0 |
| MsG0280007061.01.T01 | MTR_4g052770 | 35.971 | 139 | 87 | 2 | 69 | 205 | 67 | 205 | 6.45e-21 | 86.7 |
| MsG0280007061.01.T01 | MTR_2g030895 | 33.140 | 172 | 105 | 5 | 44 | 205 | 7 | 178 | 1.91e-20 | 84.7 |
| MsG0280007061.01.T01 | MTR_3g096330 | 33.149 | 181 | 105 | 5 | 8 | 175 | 560 | 737 | 4.11e-20 | 88.2 |
| MsG0280007061.01.T01 | MTR_2g086640 | 35.000 | 140 | 87 | 4 | 69 | 205 | 65 | 203 | 7.68e-20 | 84.0 |
| MsG0280007061.01.T01 | MTR_6g005350 | 31.383 | 188 | 120 | 5 | 26 | 205 | 28 | 214 | 2.53e-19 | 82.8 |
| MsG0280007061.01.T01 | MTR_2g030865 | 31.977 | 172 | 107 | 5 | 44 | 205 | 7 | 178 | 4.48e-18 | 78.6 |
| MsG0280007061.01.T01 | MTR_2g031300 | 37.736 | 106 | 59 | 3 | 44 | 142 | 7 | 112 | 4.94e-13 | 64.7 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MsG0280007061.01.T01 | AT1G72610 | 47.423 | 194 | 96 | 4 | 21 | 209 | 16 | 208 | 2.08e-59 | 186 |
| MsG0280007061.01.T01 | AT5G20630 | 43.192 | 213 | 113 | 5 | 1 | 208 | 1 | 210 | 1.28e-50 | 163 |
| MsG0280007061.01.T01 | AT5G39160 | 34.928 | 209 | 122 | 5 | 7 | 205 | 11 | 215 | 5.34e-34 | 121 |
| MsG0280007061.01.T01 | AT5G39110 | 36.916 | 214 | 124 | 5 | 4 | 206 | 8 | 221 | 5.43e-34 | 121 |
| MsG0280007061.01.T01 | AT5G39120 | 36.036 | 222 | 120 | 7 | 1 | 206 | 5 | 220 | 1.51e-33 | 120 |
| MsG0280007061.01.T01 | AT5G39190 | 34.450 | 209 | 127 | 4 | 7 | 205 | 11 | 219 | 4.89e-33 | 118 |
| MsG0280007061.01.T01 | AT5G39160 | 34.450 | 209 | 127 | 4 | 7 | 205 | 11 | 219 | 5.05e-33 | 118 |
| MsG0280007061.01.T01 | AT5G39130 | 33.971 | 209 | 128 | 4 | 7 | 205 | 11 | 219 | 2.05e-32 | 117 |
| MsG0280007061.01.T01 | AT5G39180 | 35.586 | 222 | 121 | 7 | 1 | 206 | 5 | 220 | 2.11e-32 | 117 |
| MsG0280007061.01.T01 | AT5G39150 | 35.586 | 222 | 121 | 7 | 1 | 206 | 5 | 220 | 6.16e-32 | 115 |
| MsG0280007061.01.T01 | AT4G14630 | 34.597 | 211 | 127 | 5 | 6 | 205 | 12 | 222 | 2.03e-31 | 114 |
| MsG0280007061.01.T01 | AT3G05950 | 35.714 | 210 | 121 | 5 | 10 | 205 | 12 | 221 | 9.33e-31 | 113 |
| MsG0280007061.01.T01 | AT1G02335 | 31.429 | 210 | 134 | 6 | 4 | 205 | 8 | 215 | 1.17e-30 | 112 |
| MsG0280007061.01.T01 | AT3G04190 | 34.021 | 194 | 118 | 4 | 22 | 205 | 28 | 221 | 1.95e-30 | 112 |
| MsG0280007061.01.T01 | AT5G38960 | 36.923 | 195 | 112 | 5 | 22 | 205 | 28 | 222 | 8.18e-30 | 110 |
| MsG0280007061.01.T01 | AT1G09560 | 35.678 | 199 | 115 | 6 | 20 | 206 | 19 | 216 | 2.38e-29 | 108 |
| MsG0280007061.01.T01 | AT5G39160 | 31.707 | 205 | 116 | 3 | 7 | 205 | 11 | 197 | 4.93e-29 | 107 |
| MsG0280007061.01.T01 | AT5G39190 | 31.707 | 205 | 116 | 3 | 7 | 205 | 11 | 197 | 5.37e-29 | 107 |
| MsG0280007061.01.T01 | AT3G04200 | 33.953 | 215 | 122 | 5 | 6 | 205 | 13 | 222 | 5.56e-29 | 108 |
| MsG0280007061.01.T01 | AT5G26700 | 33.173 | 208 | 129 | 6 | 3 | 205 | 7 | 209 | 5.66e-29 | 107 |
| MsG0280007061.01.T01 | AT1G18970 | 35.149 | 202 | 125 | 5 | 9 | 205 | 18 | 218 | 1.03e-28 | 107 |
| MsG0280007061.01.T01 | AT3G05930 | 33.178 | 214 | 131 | 7 | 1 | 205 | 5 | 215 | 1.62e-28 | 107 |
| MsG0280007061.01.T01 | AT1G18980 | 37.097 | 186 | 109 | 6 | 26 | 205 | 35 | 218 | 1.90e-28 | 106 |
| MsG0280007061.01.T01 | AT3G04180 | 33.333 | 201 | 110 | 5 | 22 | 205 | 28 | 221 | 2.84e-28 | 106 |
| MsG0280007061.01.T01 | AT3G04150 | 30.769 | 195 | 125 | 4 | 25 | 209 | 31 | 225 | 1.64e-27 | 104 |
| MsG0280007061.01.T01 | AT3G04170 | 31.905 | 210 | 130 | 5 | 6 | 205 | 12 | 218 | 5.85e-27 | 103 |
| MsG0280007061.01.T01 | AT5G38940 | 35.385 | 195 | 116 | 5 | 22 | 206 | 28 | 222 | 8.87e-26 | 100 |
| MsG0280007061.01.T01 | AT5G38940 | 35.385 | 195 | 116 | 5 | 22 | 206 | 52 | 246 | 1.29e-25 | 100 |
| MsG0280007061.01.T01 | AT3G62020 | 31.746 | 189 | 120 | 6 | 25 | 205 | 28 | 215 | 6.61e-25 | 97.4 |
| MsG0280007061.01.T01 | AT3G10080 | 33.854 | 192 | 113 | 4 | 25 | 205 | 31 | 219 | 7.23e-25 | 97.8 |
| MsG0280007061.01.T01 | AT3G04150 | 30.682 | 176 | 112 | 4 | 44 | 209 | 63 | 238 | 9.90e-25 | 97.8 |
| MsG0280007061.01.T01 | AT3G10080 | 33.333 | 189 | 118 | 3 | 25 | 205 | 102 | 290 | 1.52e-24 | 98.2 |
| MsG0280007061.01.T01 | AT5G38910 | 33.649 | 211 | 130 | 5 | 5 | 205 | 9 | 219 | 1.91e-24 | 96.3 |
| MsG0280007061.01.T01 | AT5G61750 | 34.503 | 171 | 104 | 5 | 4 | 168 | 5 | 173 | 2.12e-24 | 95.9 |
| MsG0280007061.01.T01 | AT3G62020 | 32.184 | 174 | 110 | 5 | 40 | 205 | 13 | 186 | 3.09e-24 | 95.1 |
| MsG0280007061.01.T01 | AT5G38930 | 33.846 | 195 | 119 | 5 | 22 | 206 | 28 | 222 | 6.19e-23 | 92.4 |
| MsG0280007061.01.T01 | AT1G74820 | 31.776 | 214 | 133 | 6 | 4 | 205 | 13 | 225 | 5.43e-22 | 90.1 |
| MsG0280007061.01.T01 | AT1G10460 | 29.897 | 194 | 125 | 7 | 25 | 207 | 24 | 217 | 1.82e-20 | 85.9 |
| MsG0280007061.01.T01 | AT5G38910 | 29.327 | 208 | 106 | 5 | 5 | 205 | 9 | 182 | 5.65e-13 | 65.1 |
Find 37 sgRNAs with CRISPR-Local
Find 41 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| GAAGCTTAAGGCTAAGTTTA+AGG | 0.120944 | 2:+10076022 | MsG0280007061.01.T01:CDS |
| TCTTAAGCCTACTGATATTA+TGG | 0.242052 | 2:+10075821 | MsG0280007061.01.T01:CDS |
| GTGAATGATTTCGTGTTATC+TGG | 0.259765 | 2:+10075570 | MsG0280007061.01.T01:CDS |
| ACAAAATGCAATAGTCCTTT+TGG | 0.270307 | 2:-10075849 | None:intergenic |
| AAAGCTTTGTTTAAGCTTCC+AGG | 0.294960 | 2:-10075600 | None:intergenic |
| TTTCTTGATTCTGTATTGTT+TGG | 0.320257 | 2:+10075939 | MsG0280007061.01.T01:CDS |
| AAGCTTAAGGCTAAGTTTAA+GGG | 0.375783 | 2:+10076023 | MsG0280007061.01.T01:CDS |
| GATTTCGTGTTATCTGGCTT+TGG | 0.389146 | 2:+10075576 | MsG0280007061.01.T01:CDS |
| TAATTCAGTAACTGATGCTT+TGG | 0.397550 | 2:-10075637 | None:intergenic |
| TTCACAGATCAAGAAGCTTA+AGG | 0.430319 | 2:+10076010 | MsG0280007061.01.T01:CDS |
| GTGTTATCTGGCTTTGGACC+TGG | 0.438197 | 2:+10075582 | MsG0280007061.01.T01:CDS |
| TGCATTGGAACAGATCCATT+TGG | 0.439588 | 2:-10075708 | None:intergenic |
| ATTAGTAATTCAGTTGCATC+AGG | 0.439691 | 2:-10075738 | None:intergenic |
| AGAGTAGACATTGGTCCAAA+TGG | 0.456817 | 2:+10075693 | MsG0280007061.01.T01:CDS |
| CTTTGACATAAACTTCTGTA+AGG | 0.459348 | 2:-10075797 | None:intergenic |
| TTTGTAGTTAACTCTGGTGC+TGG | 0.460867 | 2:+10075867 | MsG0280007061.01.T01:CDS |
| TGCAACTGAATTACTAATCA+TGG | 0.474013 | 2:+10075743 | MsG0280007061.01.T01:CDS |
| AAAACTCGAGATTCGCCTTC+AGG | 0.477822 | 2:+10075525 | MsG0280007061.01.T01:CDS |
| AAGGCTAAGTTTAAGGGCAC+TGG | 0.487685 | 2:+10076029 | MsG0280007061.01.T01:CDS |
| GTCTTTGCAATTATAGAAGT+AGG | 0.493739 | 2:-10075972 | None:intergenic |
| GTTTGCTACACAGAAATCAT+TGG | 0.495061 | 2:-10075496 | None:intergenic |
| CATTGGCAGAAGATGAAACA+TGG | 0.504978 | 2:-10075479 | None:intergenic |
| GAATCAAGAAACTGAAAGCT+AGG | 0.508983 | 2:-10075927 | None:intergenic |
| TTCACCGTTGGTGATTTGCA+TGG | 0.518005 | 2:-10075552 | None:intergenic |
| TAAGTTTAAGGGCACTGGCT+AGG | 0.526606 | 2:+10076034 | MsG0280007061.01.T01:CDS |
| GTTCAAGGTAGAGTTACAGC+TGG | 0.531322 | 2:+10075765 | MsG0280007061.01.T01:CDS |
| ACTGAATTACCAAGTCTCAA+TGG | 0.535559 | 2:+10075651 | MsG0280007061.01.T01:CDS |
| GAATTACTAATCATGGTTCA+AGG | 0.537186 | 2:+10075750 | MsG0280007061.01.T01:CDS |
| GGGAGGTGTATGATGATATG+AGG | 0.543192 | 2:-10075458 | None:intergenic |
| GCATCAGGATGAGTATGCAT+TGG | 0.555789 | 2:-10075723 | None:intergenic |
| ATATCAAGTCCATTGAGACT+TGG | 0.613542 | 2:-10075660 | None:intergenic |
| AACACGAAATCATTCACCGT+TGG | 0.627838 | 2:-10075564 | None:intergenic |
| ATTGGCAGAAGATGAAACAT+GGG | 0.653550 | 2:-10075478 | None:intergenic |
| GGCAGAAGATGAAACATGGG+AGG | 0.675496 | 2:-10075475 | None:intergenic |
| TCTGCAGCAAGAGTAGACAT+TGG | 0.676542 | 2:+10075684 | MsG0280007061.01.T01:CDS |
| CTATCCATGCAAATCACCAA+CGG | 0.680168 | 2:+10075548 | MsG0280007061.01.T01:CDS |
| GATTTGCATGGATAGCCTGA+AGG | 0.705863 | 2:-10075540 | None:intergenic |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|---|---|---|---|---|
| ! | TTTCTTGATTCTGTATTGTT+TGG | + | Chr2:10075939-10075958 | MsG0280007061.01.T01:CDS | 25.0% |
| !! | GATATTATGGTTTTTCCAAA+AGG | + | Chr2:10075834-10075853 | MsG0280007061.01.T01:CDS | 25.0% |
| AAGCTTAAGGCTAAGTTTAA+GGG | + | Chr2:10076023-10076042 | MsG0280007061.01.T01:CDS | 30.0% | |
| ATTAGTAATTCAGTTGCATC+AGG | - | Chr2:10075741-10075760 | None:intergenic | 30.0% | |
| CTTTGACATAAACTTCTGTA+AGG | - | Chr2:10075800-10075819 | None:intergenic | 30.0% | |
| GAATTACTAATCATGGTTCA+AGG | + | Chr2:10075750-10075769 | MsG0280007061.01.T01:CDS | 30.0% | |
| GGAAAAACCATAATATCAGT+AGG | - | Chr2:10075831-10075850 | None:intergenic | 30.0% | |
| GTCTTTGCAATTATAGAAGT+AGG | - | Chr2:10075975-10075994 | None:intergenic | 30.0% | |
| TAATTCAGTAACTGATGCTT+TGG | - | Chr2:10075640-10075659 | None:intergenic | 30.0% | |
| TCTTAAGCCTACTGATATTA+TGG | + | Chr2:10075821-10075840 | MsG0280007061.01.T01:CDS | 30.0% | |
| TGCAACTGAATTACTAATCA+TGG | + | Chr2:10075743-10075762 | MsG0280007061.01.T01:CDS | 30.0% | |
| ! | ACAAAATGCAATAGTCCTTT+TGG | - | Chr2:10075852-10075871 | None:intergenic | 30.0% |
| ! | TTGCATTTTGTAGTTAACTC+TGG | + | Chr2:10075861-10075880 | MsG0280007061.01.T01:CDS | 30.0% |
| ACTGAATTACCAAGTCTCAA+TGG | + | Chr2:10075651-10075670 | MsG0280007061.01.T01:CDS | 35.0% | |
| ATATCAAGTCCATTGAGACT+TGG | - | Chr2:10075663-10075682 | None:intergenic | 35.0% | |
| ATTGGCAGAAGATGAAACAT+GGG | - | Chr2:10075481-10075500 | None:intergenic | 35.0% | |
| GAAGCTTAAGGCTAAGTTTA+AGG | + | Chr2:10076022-10076041 | MsG0280007061.01.T01:CDS | 35.0% | |
| GAATCAAGAAACTGAAAGCT+AGG | - | Chr2:10075930-10075949 | None:intergenic | 35.0% | |
| GTGAATGATTTCGTGTTATC+TGG | + | Chr2:10075570-10075589 | MsG0280007061.01.T01:CDS | 35.0% | |
| TGATATGAGGAGAGCAAAAA+AGG | - | Chr2:10075448-10075467 | None:intergenic | 35.0% | |
| TTCACAGATCAAGAAGCTTA+AGG | + | Chr2:10076010-10076029 | MsG0280007061.01.T01:CDS | 35.0% | |
| ! | GTTTGCTACACAGAAATCAT+TGG | - | Chr2:10075499-10075518 | None:intergenic | 35.0% |
| !! | AAAGCTTTGTTTAAGCTTCC+AGG | - | Chr2:10075603-10075622 | None:intergenic | 35.0% |
| AACACGAAATCATTCACCGT+TGG | - | Chr2:10075567-10075586 | None:intergenic | 40.0% | |
| AGAGTAGACATTGGTCCAAA+TGG | + | Chr2:10075693-10075712 | MsG0280007061.01.T01:CDS | 40.0% | |
| CATTGGCAGAAGATGAAACA+TGG | - | Chr2:10075482-10075501 | None:intergenic | 40.0% | |
| CTATCCATGCAAATCACCAA+CGG | + | Chr2:10075548-10075567 | MsG0280007061.01.T01:CDS | 40.0% | |
| TGCATTGGAACAGATCCATT+TGG | - | Chr2:10075711-10075730 | None:intergenic | 40.0% | |
| TTTGTAGTTAACTCTGGTGC+TGG | + | Chr2:10075867-10075886 | MsG0280007061.01.T01:CDS | 40.0% | |
| ! | GATTTCGTGTTATCTGGCTT+TGG | + | Chr2:10075576-10075595 | MsG0280007061.01.T01:CDS | 40.0% |
| AAAACTCGAGATTCGCCTTC+AGG | + | Chr2:10075525-10075544 | MsG0280007061.01.T01:CDS | 45.0% | |
| GATTTGCATGGATAGCCTGA+AGG | - | Chr2:10075543-10075562 | None:intergenic | 45.0% | |
| GCATCAGGATGAGTATGCAT+TGG | - | Chr2:10075726-10075745 | None:intergenic | 45.0% | |
| GTTCAAGGTAGAGTTACAGC+TGG | + | Chr2:10075765-10075784 | MsG0280007061.01.T01:CDS | 45.0% | |
| TCTGCAGCAAGAGTAGACAT+TGG | + | Chr2:10075684-10075703 | MsG0280007061.01.T01:CDS | 45.0% | |
| ! | AAGGCTAAGTTTAAGGGCAC+TGG | + | Chr2:10076029-10076048 | MsG0280007061.01.T01:CDS | 45.0% |
| ! | GGGAGGTGTATGATGATATG+AGG | - | Chr2:10075461-10075480 | None:intergenic | 45.0% |
| !! | GCGAATCTCGAGTTTTGTGT+AGG | - | Chr2:10075521-10075540 | None:intergenic | 45.0% |
| !! | TTCACCGTTGGTGATTTGCA+TGG | - | Chr2:10075555-10075574 | None:intergenic | 45.0% |
| GGCAGAAGATGAAACATGGG+AGG | - | Chr2:10075478-10075497 | None:intergenic | 50.0% | |
| !! | GTGTTATCTGGCTTTGGACC+TGG | + | Chr2:10075582-10075601 | MsG0280007061.01.T01:CDS | 50.0% |
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| Chr2 | gene | 10075426 | 10076055 | 10075426 | ID=MsG0280007061.01;Name=MsG0280007061.01 |
| Chr2 | mRNA | 10075426 | 10076055 | 10075426 | ID=MsG0280007061.01.T01;Parent=MsG0280007061.01;Name=MsG0280007061.01.T01;_AED=0.13;_eAED=0.13;_QI=0|-1|0|1|-1|1|1|0|209 |
| Chr2 | exon | 10075426 | 10076055 | 10075426 | ID=MsG0280007061.01.T01:exon:6360;Parent=MsG0280007061.01.T01 |
| Chr2 | CDS | 10075426 | 10076055 | 10075426 | ID=MsG0280007061.01.T01:cds;Parent=MsG0280007061.01.T01 |
| Gene Sequence |
| Protein sequence |
