AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar ZhongmuNo.1 / MsG0580028527.01


Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MsG0580028527.01.T01 MTR_5g073450 75.824 182 44 0 1 182 455 636 1.04e-94 288
MsG0580028527.01.T01 MTR_5g073460 68.478 184 58 0 1 184 514 697 3.24e-81 254
MsG0580028527.01.T01 MTR_3g031130 63.587 184 60 1 1 184 448 624 6.20e-74 234
MsG0580028527.01.T01 MTR_5g073490 59.172 169 54 1 13 181 3 156 4.77e-65 197
MsG0580028527.01.T01 MTR_5g073780 48.295 176 89 1 5 180 51 224 1.29e-44 147
MsG0580028527.01.T01 MTR_4g103540 46.369 179 95 1 4 182 506 683 1.64e-43 153
MsG0580028527.01.T01 MTR_5g073620 48.864 176 88 1 5 180 896 1069 8.63e-42 149
MsG0580028527.01.T01 MTR_5g073620 51.220 82 36 1 4 81 528 609 1.90e-18 82.8
MsG0580028527.01.T01 MTR_6g033310 40.110 182 106 2 4 184 549 728 2.65e-41 147
MsG0580028527.01.T01 MTR_7g026630 41.899 179 98 4 4 179 475 650 7.58e-41 146
MsG0580028527.01.T01 MTR_1g117090 45.251 179 95 3 4 180 492 669 1.72e-40 145
MsG0580028527.01.T01 MTR_8g021330 41.989 181 101 2 3 179 425 605 2.85e-40 144
MsG0580028527.01.T01 MTR_5g073920 45.000 180 93 2 1 180 487 660 5.01e-40 144
MsG0580028527.01.T01 MTR_1g090620 38.743 191 102 2 4 179 451 641 2.33e-39 142
MsG0580028527.01.T01 MTR_5g074130 45.810 179 93 2 1 177 497 673 2.46e-38 139
MsG0580028527.01.T01 MTR_1g057560 38.798 183 107 1 4 181 445 627 3.20e-37 135
MsG0580028527.01.T01 MTR_7g013770 42.286 175 95 2 7 180 498 667 2.68e-36 133
MsG0580028527.01.T01 MTR_5g093530 42.938 177 78 2 1 177 417 570 1.96e-34 127
MsG0580028527.01.T01 MTR_3g464010 40.909 176 97 3 4 177 496 666 1.16e-30 117
MsG0580028527.01.T01 MTR_3g025900 34.831 178 107 3 4 180 440 609 3.74e-25 101
MsG0580028527.01.T01 MTR_5g073480 38.857 175 42 4 1 173 514 625 9.15e-25 100
MsG0580028527.01.T01 MTR_2g096230 32.967 182 108 3 1 182 417 584 9.99e-24 97.8
MsG0580028527.01.T01 MTR_5g073900 39.597 149 67 2 1 149 220 345 2.82e-21 89.4
MsG0580028527.01.T01 MTR_1g067460 28.249 177 123 1 1 177 427 599 9.96e-20 86.3
MsG0580028527.01.T01 MTR_4g062330 32.000 175 109 3 3 177 438 602 3.01e-19 84.7
MsG0580028527.01.T01 MTR_1g067480 30.508 177 118 3 1 177 429 600 9.33e-19 83.6
MsG0580028527.01.T01 MTR_0229s0040 28.415 183 117 4 4 178 31 207 2.44e-18 79.7
MsG0580028527.01.T01 MTR_5g074190 37.589 141 53 4 38 178 303 408 2.96e-17 79.0
MsG0580028527.01.T01 MTR_1g009620 28.415 183 117 4 4 178 476 652 5.68e-17 78.2
MsG0580028527.01.T01 MTR_1g017910 31.847 157 100 4 4 153 507 663 2.36e-14 70.9
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MsG0580028527.01.T01 AT5G58430 59.669 181 72 1 1 181 441 620 1.63e-67 217
MsG0580028527.01.T01 AT1G07000 43.889 180 92 2 3 181 427 598 3.78e-47 162
MsG0580028527.01.T01 AT5G13150 44.809 183 95 4 1 180 466 645 5.11e-47 163
MsG0580028527.01.T01 AT1G72470 45.652 184 96 3 3 182 441 624 2.54e-45 158
MsG0580028527.01.T01 AT5G13990 41.989 181 99 3 4 181 506 683 2.11e-44 156
MsG0580028527.01.T01 AT3G14090 41.081 185 105 2 3 183 433 617 9.11e-42 148
MsG0580028527.01.T01 AT5G50380 43.169 183 94 1 8 180 490 672 2.28e-41 147
MsG0580028527.01.T01 AT1G54090 39.683 189 109 3 1 184 429 617 9.38e-40 142
MsG0580028527.01.T01 AT5G03540 38.776 196 93 3 4 181 331 517 1.55e-39 141
MsG0580028527.01.T01 AT5G52340 39.779 181 106 1 4 181 445 625 2.89e-39 141
MsG0580028527.01.T01 AT5G03540 38.776 196 93 3 4 181 446 632 3.59e-39 141
MsG0580028527.01.T01 AT5G52350 40.110 182 106 2 1 179 396 577 7.04e-39 140
MsG0580028527.01.T01 AT5G03540 38.776 196 93 3 4 181 472 658 7.11e-39 140
MsG0580028527.01.T01 AT3G29400 38.798 183 102 2 4 181 472 649 1.80e-29 114
MsG0580028527.01.T01 AT5G61010 36.111 180 103 4 4 183 463 630 1.97e-28 111
MsG0580028527.01.T01 AT5G61010 36.111 180 103 4 4 183 463 630 1.97e-28 111
MsG0580028527.01.T01 AT2G39380 31.111 180 120 2 1 180 436 611 7.90e-25 100
MsG0580028527.01.T01 AT1G07725 32.402 179 108 4 1 179 426 591 3.50e-24 99.0
MsG0580028527.01.T01 AT3G09530 30.168 179 121 3 3 179 426 602 7.59e-23 95.1
MsG0580028527.01.T01 AT2G28640 29.775 178 115 2 3 180 412 579 1.01e-20 89.0
MsG0580028527.01.T01 AT3G09520 32.222 180 114 3 3 178 430 605 1.68e-20 88.6
MsG0580028527.01.T01 AT5G59730 32.222 180 114 3 3 180 426 599 3.92e-20 87.4
MsG0580028527.01.T01 AT5G59730 32.778 180 113 4 3 180 426 599 4.40e-20 87.4
MsG0580028527.01.T01 AT3G55150 28.090 178 123 2 3 180 439 611 8.74e-19 83.6
MsG0580028527.01.T01 AT4G31540 27.473 182 118 4 4 177 479 654 6.68e-17 78.2
MsG0580028527.01.T01 AT2G28650 31.138 167 98 3 12 177 404 554 3.91e-14 70.1

Find 35 sgRNAs with CRISPR-Local

Find 38 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
GATACTCGACGATTTGTTTC+AGG 0.232465 5:+81877454 MsG0580028527.01.T01:CDS
AGCAGGGTCACTATAGCTTT+TGG 0.271459 5:-81876988 None:intergenic
GCTGATAAATATATTAAGTA+TGG 0.315006 5:+81877413 MsG0580028527.01.T01:CDS
TCAAAGAAGCTCATGGAATA+AGG 0.330223 5:+81877145 MsG0580028527.01.T01:CDS
AGTCCAGATGGATTGGATTA+TGG 0.334766 5:+81876935 None:intergenic
TATGCAGGGTTCAATCTTCA+TGG 0.357745 5:+81877273 MsG0580028527.01.T01:CDS
GTGATTCAATGCAGACTAAT+GGG 0.363928 5:+81877195 MsG0580028527.01.T01:CDS
CTTCACTTTCGGCTACCAAT+TGG 0.364033 5:-81877489 None:intergenic
GGAGCTATTAGAGAGCAATT+TGG 0.368249 5:+81876956 MsG0580028527.01.T01:CDS
AGTGATTCAATGCAGACTAA+TGG 0.406980 5:+81877194 MsG0580028527.01.T01:CDS
TTCACTTTCGGCTACCAATT+GGG 0.415369 5:-81877488 None:intergenic
TTATCTTTGATAAACACCTT+GGG 0.417322 5:+81877297 MsG0580028527.01.T01:CDS
CTTTATCAGTCCAGATGGAT+TGG 0.425264 5:+81876928 None:intergenic
AGGGAAGCAGTGGACCCAAT+TGG 0.486311 5:+81877474 MsG0580028527.01.T01:CDS
TTTCAGAGAATTCTTGAACT+TGG 0.490821 5:+81877383 MsG0580028527.01.T01:CDS
TTTATCTTTGATAAACACCT+TGG 0.500848 5:+81877296 MsG0580028527.01.T01:CDS
CCGATGAAGTTTCTATAAGC+TGG 0.501074 5:-81877356 None:intergenic
TGCTCTTTGAGAAAATATGC+AGG 0.504818 5:+81877258 MsG0580028527.01.T01:CDS
ATACTCGACGATTTGTTTCA+GGG 0.512113 5:+81877455 MsG0580028527.01.T01:CDS
AAGAAAACATAGCACAAAGC+AGG 0.512116 5:-81877005 None:intergenic
AAGCTCATGGAATAAGGTGT+TGG 0.533766 5:+81877151 MsG0580028527.01.T01:CDS
CAGCTTATAGAAACTTCATC+GGG 0.540767 5:+81877357 MsG0580028527.01.T01:CDS
TAAATATATTAAGTATGGAA+TGG 0.546431 5:+81877418 MsG0580028527.01.T01:CDS
CCAGCTTATAGAAACTTCAT+CGG 0.549452 5:+81877356 MsG0580028527.01.T01:CDS
AAAATCTTATTTCTTCCCCA+AGG 0.555068 5:-81877313 None:intergenic
AGCTTCTTTGATAGTGCTCA+CGG 0.555273 5:-81877133 None:intergenic
GATTTGTTTCAGGGAAGCAG+TGG 0.571987 5:+81877464 MsG0580028527.01.T01:CDS
AGCACTATCAAAGAAGCTCA+TGG 0.584643 5:+81877138 MsG0580028527.01.T01:CDS
ATATATTAAGTATGGAATGG+AGG 0.587729 5:+81877421 MsG0580028527.01.T01:CDS
CTTATAGAAACTTCATCGGG+AGG 0.598813 5:+81877360 MsG0580028527.01.T01:CDS
TATCTTTGATAAACACCTTG+GGG 0.629477 5:+81877298 MsG0580028527.01.T01:CDS
TGGCAACAAGAGTTTCAGAA+TGG 0.629756 5:-81877336 None:intergenic
GCTCTTTGAGAAAATATGCA+GGG 0.633356 5:+81877259 MsG0580028527.01.T01:CDS
AGAAAACATAGCACAAAGCA+GGG 0.634160 5:-81877004 None:intergenic
TGATTCAATGCAGACTAATG+GGG 0.659599 5:+81877196 MsG0580028527.01.T01:CDS

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!! TAAATATATTAAGTATGGAA+TGG + Chr5:81877418-81877437 MsG0580028527.01.T01:CDS 15.0%
!! GCTGATAAATATATTAAGTA+TGG + Chr5:81877413-81877432 MsG0580028527.01.T01:CDS 20.0%
!!! AGTGAATTAGAAACTATTTT+AGG + Chr5:81877068-81877087 MsG0580028527.01.T01:CDS 20.0%
!!! GTGAATTAGAAACTATTTTA+GGG + Chr5:81877069-81877088 MsG0580028527.01.T01:CDS 20.0%
!!! TGAATTAGAAACTATTTTAG+GGG + Chr5:81877070-81877089 MsG0580028527.01.T01:CDS 20.0%
! ATATATTAAGTATGGAATGG+AGG + Chr5:81877421-81877440 MsG0580028527.01.T01:CDS 25.0%
! TTATCTTTGATAAACACCTT+GGG + Chr5:81877297-81877316 MsG0580028527.01.T01:CDS 25.0%
! TTTATCTTTGATAAACACCT+TGG + Chr5:81877296-81877315 MsG0580028527.01.T01:CDS 25.0%
AAAAACACACTGCAAAAGTT+CGG + Chr5:81877105-81877124 MsG0580028527.01.T01:CDS 30.0%
TATCTTTGATAAACACCTTG+GGG + Chr5:81877298-81877317 MsG0580028527.01.T01:CDS 30.0%
TTTCAGAGAATTCTTGAACT+TGG + Chr5:81877383-81877402 MsG0580028527.01.T01:CDS 30.0%
! AAAATCTTATTTCTTCCCCA+AGG - Chr5:81877316-81877335 None:intergenic 30.0%
AAGAAAACATAGCACAAAGC+AGG - Chr5:81877008-81877027 None:intergenic 35.0%
AGAAAACATAGCACAAAGCA+GGG - Chr5:81877007-81877026 None:intergenic 35.0%
AGTGATTCAATGCAGACTAA+TGG + Chr5:81877194-81877213 MsG0580028527.01.T01:CDS 35.0%
CAGCTTATAGAAACTTCATC+GGG + Chr5:81877357-81877376 MsG0580028527.01.T01:CDS 35.0%
CCAGCTTATAGAAACTTCAT+CGG + Chr5:81877356-81877375 MsG0580028527.01.T01:CDS 35.0%
GTGATTCAATGCAGACTAAT+GGG + Chr5:81877195-81877214 MsG0580028527.01.T01:CDS 35.0%
TCAAAGAAGCTCATGGAATA+AGG + Chr5:81877145-81877164 MsG0580028527.01.T01:CDS 35.0%
TGATTCAATGCAGACTAATG+GGG + Chr5:81877196-81877215 MsG0580028527.01.T01:CDS 35.0%
! ATACTCGACGATTTGTTTCA+GGG + Chr5:81877455-81877474 MsG0580028527.01.T01:CDS 35.0%
! GAACTTTTGCAGTGTGTTTT+TGG - Chr5:81877106-81877125 None:intergenic 35.0%
! GCTCTTTGAGAAAATATGCA+GGG + Chr5:81877259-81877278 MsG0580028527.01.T01:CDS 35.0%
! GTCACTATAGCTTTTGGATT+TGG - Chr5:81876985-81877004 None:intergenic 35.0%
! TGCTCTTTGAGAAAATATGC+AGG + Chr5:81877258-81877277 MsG0580028527.01.T01:CDS 35.0%
AAGCTCATGGAATAAGGTGT+TGG + Chr5:81877151-81877170 MsG0580028527.01.T01:CDS 40.0%
AGCACTATCAAAGAAGCTCA+TGG + Chr5:81877138-81877157 MsG0580028527.01.T01:CDS 40.0%
CCGATGAAGTTTCTATAAGC+TGG - Chr5:81877359-81877378 None:intergenic 40.0%
CTTATAGAAACTTCATCGGG+AGG + Chr5:81877360-81877379 MsG0580028527.01.T01:CDS 40.0%
GGAGCTATTAGAGAGCAATT+TGG + Chr5:81876956-81876975 MsG0580028527.01.T01:CDS 40.0%
TATGCAGGGTTCAATCTTCA+TGG + Chr5:81877273-81877292 MsG0580028527.01.T01:CDS 40.0%
TGGCAACAAGAGTTTCAGAA+TGG - Chr5:81877339-81877358 None:intergenic 40.0%
! GATACTCGACGATTTGTTTC+AGG + Chr5:81877454-81877473 MsG0580028527.01.T01:CDS 40.0%
!! AGCTTCTTTGATAGTGCTCA+CGG - Chr5:81877136-81877155 None:intergenic 40.0%
!!! GGTGTTGGACTTTTTGAAGT+TGG + Chr5:81877166-81877185 MsG0580028527.01.T01:CDS 40.0%
! AGCAGGGTCACTATAGCTTT+TGG - Chr5:81876991-81877010 None:intergenic 45.0%
! GATTTGTTTCAGGGAAGCAG+TGG + Chr5:81877464-81877483 MsG0580028527.01.T01:CDS 45.0%
AGGGAAGCAGTGGACCCAAT+TGG + Chr5:81877474-81877493 MsG0580028527.01.T01:CDS 55.0%
Chromosome Type Strat End Strand Name
Chr5 gene 81876942 81877499 81876942 ID=MsG0580028527.01;Name=MsG0580028527.01
Chr5 mRNA 81876942 81877499 81876942 ID=MsG0580028527.01.T01;Parent=MsG0580028527.01;Name=MsG0580028527.01.T01;_AED=0.47;_eAED=0.47;_QI=0|-1|0|1|-1|1|1|0|185
Chr5 exon 81876942 81877499 81876942 ID=MsG0580028527.01.T01:exon:21075;Parent=MsG0580028527.01.T01
Chr5 CDS 81876942 81877499 81876942 ID=MsG0580028527.01.T01:cds;Parent=MsG0580028527.01.T01
Gene Sequence

>MsG0580028527.01.T01

ATGGATTGGATTATGGAGCTATTAGAGAGCAATTTGGAAGCCAAATCCAAAAGCTATAGTGACCCTGCTTTGTGCTATGTTTTCTTGATGAATAACTGCAGCTATATTGTTCAGAAGACTGAAGATAGTGAATTAGAAACTATTTTAGGGGATGTTGTGATCCAAAAACACACTGCAAAAGTTCGGCAATACCGTGAGCACTATCAAAGAAGCTCATGGAATAAGGTGTTGGACTTTTTGAAGTTGGACAACAGTGATTCAATGCAGACTAATGGGGTAGAAAAGTCAACGAAAAATAAGTTAAAATCATTCAACATGCTCTTTGAGAAAATATGCAGGGTTCAATCTTCATGGTTTATCTTTGATAAACACCTTGGGGAAGAAATAAGATTTTCCATTCTGAAACTCTTGTTGCCAGCTTATAGAAACTTCATCGGGAGGTTTCAGAGAATTCTTGAACTTGGTAAGCGTGCTGATAAATATATTAAGTATGGAATGGAGGATATCGAAGCGATACTCGACGATTTGTTTCAGGGAAGCAGTGGACCCAATTGGTAG

Protein sequence

>MsG0580028527.01.T01

MDWIMELLESNLEAKSKSYSDPALCYVFLMNNCSYIVQKTEDSELETILGDVVIQKHTAKVRQYREHYQRSSWNKVLDFLKLDNSDSMQTNGVEKSTKNKLKSFNMLFEKICRVQSSWFIFDKHLGEEIRFSILKLLLPAYRNFIGRFQRILELGKRADKYIKYGMEDIEAILDDLFQGSSGPNW*