Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MsG0280007110.01.T01 | RHN72370.1 | 90.909 | 154 | 14 | 0 | 9 | 162 | 16 | 169 | 3.55E-101 | 298 |
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MsG0280007110.01.T01 | Q10L32 | 80.147 | 136 | 25 | 1 | 24 | 157 | 1 | 136 | 6.49E-81 | 238 |
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MsG0280007110.01.T01 | A0A396J3C5 | 90.909 | 154 | 14 | 0 | 9 | 162 | 16 | 169 | 1.70e-101 | 298 |
| Gene ID | Type | Classification |
|---|
| Gene ID | Type | Classification |
|---|
Co-expression Network
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|---|---|---|---|
| MsG0280007110.01 | MsG0280007327.01 | 0.800532 | 1.378702e-48 | 6.434931e-46 |
PPI
| Gene1 | Gene2 | Type |
|---|
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MsG0280007110.01.T01 | MTR_2g020930 | 96.403 | 139 | 5 | 0 | 24 | 162 | 1 | 139 | 5.83e-100 | 284 |
| MsG0280007110.01.T01 | MTR_2g020950 | 92.086 | 139 | 10 | 1 | 24 | 162 | 1 | 138 | 3.64e-92 | 264 |
| MsG0280007110.01.T01 | MTR_4g092800 | 77.206 | 136 | 31 | 0 | 23 | 158 | 54 | 189 | 1.33e-78 | 231 |
| MsG0280007110.01.T01 | MTR_2g020900 | 74.809 | 131 | 32 | 1 | 32 | 162 | 20 | 149 | 4.41e-70 | 208 |
| MsG0280007110.01.T01 | MTR_8g461030 | 43.200 | 125 | 66 | 2 | 34 | 155 | 80 | 202 | 2.75e-30 | 109 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MsG0280007110.01.T01 | AT4G04830 | 76.978 | 139 | 32 | 0 | 24 | 162 | 1 | 139 | 1.04e-80 | 235 |
| MsG0280007110.01.T01 | AT4G21860 | 78.986 | 138 | 28 | 1 | 23 | 159 | 64 | 201 | 7.69e-80 | 235 |
| MsG0280007110.01.T01 | AT4G21860 | 78.986 | 138 | 28 | 1 | 23 | 159 | 64 | 201 | 7.69e-80 | 235 |
| MsG0280007110.01.T01 | AT4G21860 | 78.986 | 138 | 28 | 1 | 23 | 159 | 64 | 201 | 7.69e-80 | 235 |
| MsG0280007110.01.T01 | AT4G04810 | 73.723 | 137 | 36 | 0 | 24 | 160 | 1 | 137 | 2.41e-76 | 224 |
| MsG0280007110.01.T01 | AT4G04800 | 69.718 | 142 | 42 | 1 | 18 | 159 | 35 | 175 | 1.21e-72 | 216 |
| MsG0280007110.01.T01 | AT4G04830 | 70.504 | 139 | 31 | 1 | 24 | 162 | 1 | 129 | 2.08e-70 | 209 |
| MsG0280007110.01.T01 | AT4G21850 | 67.176 | 131 | 43 | 0 | 23 | 153 | 7 | 137 | 3.73e-68 | 203 |
| MsG0280007110.01.T01 | AT4G21840 | 63.380 | 142 | 46 | 1 | 24 | 159 | 1 | 142 | 2.11e-67 | 201 |
| MsG0280007110.01.T01 | AT4G21830 | 70.161 | 124 | 37 | 0 | 30 | 153 | 11 | 134 | 1.74e-66 | 199 |
| MsG0280007110.01.T01 | AT4G21830 | 70.161 | 124 | 37 | 0 | 30 | 153 | 14 | 137 | 5.27e-66 | 198 |
| MsG0280007110.01.T01 | AT4G04840 | 63.768 | 138 | 50 | 0 | 16 | 153 | 9 | 146 | 8.75e-65 | 195 |
| MsG0280007110.01.T01 | AT4G04810 | 74.118 | 85 | 22 | 0 | 24 | 108 | 1 | 85 | 8.59e-45 | 143 |
| MsG0280007110.01.T01 | AT4G21860 | 72.222 | 90 | 25 | 0 | 25 | 114 | 67 | 156 | 3.25e-44 | 144 |
| MsG0280007110.01.T01 | AT4G21850 | 61.628 | 86 | 33 | 0 | 23 | 108 | 7 | 92 | 1.96e-39 | 130 |
| MsG0280007110.01.T01 | AT1G53670 | 47.154 | 123 | 60 | 3 | 34 | 153 | 74 | 194 | 2.37e-33 | 117 |
| MsG0280007110.01.T01 | AT1G53670 | 41.463 | 123 | 60 | 4 | 34 | 153 | 74 | 187 | 7.28e-26 | 97.8 |
Find 51 sgRNAs with CRISPR-Local
Find 63 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| ATTAAATTTGTGAACGTTTC+AGG | 0.128184 | 2:+10780546 | None:intergenic |
| ATAATTACTCAGTTCCTTTC+TGG | 0.153041 | 2:+10780361 | None:intergenic |
| CCTTATAACAATAACTGTTT+TGG | 0.222670 | 2:+10779605 | None:intergenic |
| CCAAAACAGTTATTGTTATA+AGG | 0.223362 | 2:-10779605 | MsG0280007110.01.T01:three_prime_UTR |
| TATGGAAGAAGTAGCATTTC+CGG | 0.273975 | 2:+10779710 | None:intergenic |
| CTTTATAATGAATGAATTAG+AGG | 0.286051 | 2:-10779677 | MsG0280007110.01.T01:three_prime_UTR |
| TCTTCTACCAAATTTGATTC+AGG | 0.294875 | 2:-10780007 | MsG0280007110.01.T01:CDS |
| GCTTTCTTTGAGGGTTTCCC+TGG | 0.323698 | 2:-10779971 | MsG0280007110.01.T01:CDS |
| GCCACAACCTGAATCAAATT+TGG | 0.333002 | 2:+10780000 | None:intergenic |
| GGCTGGCCTGCTTTCTTTGA+GGG | 0.344700 | 2:-10779980 | MsG0280007110.01.T01:CDS |
| AGCTTGTAGTCAGTCTTGTT+TGG | 0.356294 | 2:-10779633 | MsG0280007110.01.T01:three_prime_UTR |
| ACCAAATTTGATTCAGGTTG+TGG | 0.370623 | 2:-10780001 | MsG0280007110.01.T01:CDS |
| TGGCTGGCCTGCTTTCTTTG+AGG | 0.381890 | 2:-10779981 | MsG0280007110.01.T01:CDS |
| TTATAAAGTAATGTTTCATA+TGG | 0.390191 | 2:+10779692 | None:intergenic |
| GTTTCTGTCAAGTTTATTCC+CGG | 0.396456 | 2:-10779729 | MsG0280007110.01.T01:CDS |
| TATAACAAGTTCTTTGAAGA+AGG | 0.421708 | 2:-10780070 | MsG0280007110.01.T01:CDS |
| CAAGCAAGTGTGCGTGCAAA+AGG | 0.427139 | 2:+10780487 | None:intergenic |
| GAAGGGATTTACAATTGTAC+TGG | 0.445202 | 2:-10780052 | MsG0280007110.01.T01:CDS |
| ATGGAAGAAGTAGCATTTCC+GGG | 0.448524 | 2:+10779711 | None:intergenic |
| ATCTCAGTCCTCCTACCATC+TGG | 0.449986 | 2:+10779843 | None:intergenic |
| GGATTACATGTGCAGATTGA+AGG | 0.451748 | 2:-10780104 | MsG0280007110.01.T01:intron |
| GTCCTCCTACCATCTGGATC+AGG | 0.457223 | 2:+10779849 | None:intergenic |
| TTGTAGTCAGTCTTGTTTGG+TGG | 0.459641 | 2:-10779630 | MsG0280007110.01.T01:three_prime_UTR |
| TCGAGAAATCTGAGGAAGAA+TGG | 0.462125 | 2:-10780422 | MsG0280007110.01.T01:CDS |
| ATTCAAAGGTGAAGGGTTCA+AGG | 0.467580 | 2:-10779784 | MsG0280007110.01.T01:CDS |
| ACAGAGCGATTGATAGCTCC+AGG | 0.473234 | 2:+10779953 | None:intergenic |
| ATGTGCAGATTGAAGGGTAC+TGG | 0.475174 | 2:-10780097 | MsG0280007110.01.T01:intron |
| AATTTGATTCAGGTTGTGGC+TGG | 0.480441 | 2:-10779997 | MsG0280007110.01.T01:CDS |
| TTGGTAGAAGACTTGTAAAG+TGG | 0.482126 | 2:+10780019 | None:intergenic |
| ATTTACAATTGTACTGGATG+TGG | 0.487952 | 2:-10780046 | MsG0280007110.01.T01:CDS |
| GGTCATGTATTCAAAGGTGA+AGG | 0.496687 | 2:-10779792 | MsG0280007110.01.T01:CDS |
| CGAAGGATGTGAAACTGCTC+AGG | 0.522030 | 2:+10780384 | None:intergenic |
| ATAACAAGTTCTTTGAAGAA+GGG | 0.522344 | 2:-10780069 | MsG0280007110.01.T01:CDS |
| ACACAATGACGTTCATCAGT+TGG | 0.524963 | 2:+10779759 | None:intergenic |
| TGTAAATCAAAATAAGTCAA+TGG | 0.526690 | 2:-10780463 | MsG0280007110.01.T01:CDS |
| GATTACATGTGCAGATTGAA+GGG | 0.530242 | 2:-10780103 | MsG0280007110.01.T01:intron |
| ACTCAGTTCCTTTCTGGCGA+AGG | 0.533241 | 2:+10780367 | None:intergenic |
| TTTCACATCCTTCGCCAGAA+AGG | 0.538872 | 2:-10780375 | MsG0280007110.01.T01:CDS |
| AAACTGCTCAGGTGAAAGAA+TGG | 0.546804 | 2:+10780395 | None:intergenic |
| GCAGCATGTGGTGGGCACTT+AGG | 0.567542 | 2:-10779813 | MsG0280007110.01.T01:CDS |
| GGGAAACCCTCAAAGAAAGC+AGG | 0.569888 | 2:+10779974 | None:intergenic |
| CACTTAGGTCATGTATTCAA+AGG | 0.577855 | 2:-10779798 | MsG0280007110.01.T01:CDS |
| GTCATGTATTCAAAGGTGAA+GGG | 0.604484 | 2:-10779791 | MsG0280007110.01.T01:CDS |
| AGCCTGATCCAGATGGTAGG+AGG | 0.605106 | 2:-10779851 | MsG0280007110.01.T01:CDS |
| TAACTTGTGCAGCATGTGGT+GGG | 0.643462 | 2:-10779821 | MsG0280007110.01.T01:CDS |
| TCAGTCTAAACTGTGCTCAG+TGG | 0.650903 | 2:+10779577 | None:intergenic |
| ATAACTTGTGCAGCATGTGG+TGG | 0.656642 | 2:-10779822 | MsG0280007110.01.T01:CDS |
| TTCAGCCTGATCCAGATGGT+AGG | 0.658719 | 2:-10779854 | MsG0280007110.01.T01:intron |
| ATCATCAATCGAGAAATCTG+AGG | 0.659784 | 2:-10780430 | MsG0280007110.01.T01:CDS |
| GAGATAACTTGTGCAGCATG+TGG | 0.695279 | 2:-10779825 | MsG0280007110.01.T01:CDS |
| CAGAGCGATTGATAGCTCCA+GGG | 0.696117 | 2:+10779954 | None:intergenic |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|---|---|---|---|---|
| !! | TATAAATCAATTATAATCAT+CGG | + | Chr2:10779857-10779876 | None:intergenic | 10.0% |
| !! | TTATAAAGTAATGTTTCATA+TGG | + | Chr2:10780418-10780437 | None:intergenic | 15.0% |
| !! | ATTAAGTTCCATTATTATCA+TGG | + | Chr2:10780225-10780244 | None:intergenic | 20.0% |
| !! | CTTTATAATGAATGAATTAG+AGG | - | Chr2:10780430-10780449 | MsG0280007110.01.T01:CDS | 20.0% |
| !! | TGTAAATCAAAATAAGTCAA+TGG | - | Chr2:10779644-10779663 | MsG0280007110.01.T01:three_prime_UTR | 20.0% |
| ! | ATACATGATAATAGAACCTT+AGG | - | Chr2:10779970-10779989 | MsG0280007110.01.T01:CDS | 25.0% |
| ! | ATTAAATTTGTGAACGTTTC+AGG | + | Chr2:10779564-10779583 | None:intergenic | 25.0% |
| ! | CCAAAACAGTTATTGTTATA+AGG | - | Chr2:10780502-10780521 | MsG0280007110.01.T01:CDS | 25.0% |
| ! | CTATGAGTAAAATCAGATTA+TGG | - | Chr2:10779803-10779822 | MsG0280007110.01.T01:CDS | 25.0% |
| ! | CTTCTTAACCATGATAATAA+TGG | - | Chr2:10780214-10780233 | MsG0280007110.01.T01:intron | 25.0% |
| !! | ATAACAAGTTCTTTGAAGAA+GGG | - | Chr2:10780038-10780057 | MsG0280007110.01.T01:CDS | 25.0% |
| !! | CCTTATAACAATAACTGTTT+TGG | + | Chr2:10780505-10780524 | None:intergenic | 25.0% |
| !! | TATAACAAGTTCTTTGAAGA+AGG | - | Chr2:10780037-10780056 | MsG0280007110.01.T01:CDS | 25.0% |
| ATAATTACTCAGTTCCTTTC+TGG | + | Chr2:10779749-10779768 | None:intergenic | 30.0% | |
| ATTTACAATTGTACTGGATG+TGG | - | Chr2:10780061-10780080 | MsG0280007110.01.T01:CDS | 30.0% | |
| TAATCCAATTTCATTGCCTA+AGG | + | Chr2:10779989-10780008 | None:intergenic | 30.0% | |
| TCTTCTACCAAATTTGATTC+AGG | - | Chr2:10780100-10780119 | MsG0280007110.01.T01:intron | 30.0% | |
| AGAACCTTAGGCAATGAAAT+TGG | - | Chr2:10779982-10780001 | MsG0280007110.01.T01:CDS | 35.0% | |
| ATCATCAATCGAGAAATCTG+AGG | - | Chr2:10779677-10779696 | MsG0280007110.01.T01:three_prime_UTR | 35.0% | |
| CACTTAGGTCATGTATTCAA+AGG | - | Chr2:10780309-10780328 | MsG0280007110.01.T01:intron | 35.0% | |
| GAAGGGATTTACAATTGTAC+TGG | - | Chr2:10780055-10780074 | MsG0280007110.01.T01:CDS | 35.0% | |
| GATTACATGTGCAGATTGAA+GGG | - | Chr2:10780004-10780023 | MsG0280007110.01.T01:CDS | 35.0% | |
| GTCATGTATTCAAAGGTGAA+GGG | - | Chr2:10780316-10780335 | MsG0280007110.01.T01:intron | 35.0% | |
| GTTTCTGTCAAGTTTATTCC+CGG | - | Chr2:10780378-10780397 | MsG0280007110.01.T01:CDS | 35.0% | |
| TATCATGCCATGAACTATTG+TGG | - | Chr2:10779922-10779941 | MsG0280007110.01.T01:intron | 35.0% | |
| TATGGAAGAAGTAGCATTTC+CGG | + | Chr2:10780400-10780419 | None:intergenic | 35.0% | |
| TTCATTGCCACAATAGTTCA+TGG | + | Chr2:10779932-10779951 | None:intergenic | 35.0% | |
| TTGGTAGAAGACTTGTAAAG+TGG | + | Chr2:10780091-10780110 | None:intergenic | 35.0% | |
| !! | ACCAAATTTGATTCAGGTTG+TGG | - | Chr2:10780106-10780125 | MsG0280007110.01.T01:intron | 35.0% |
| AAACTGCTCAGGTGAAAGAA+TGG | + | Chr2:10779715-10779734 | None:intergenic | 40.0% | |
| ACACAATGACGTTCATCAGT+TGG | + | Chr2:10780351-10780370 | None:intergenic | 40.0% | |
| ATCTGGATCAGGCTGAAAAT+GGG | + | Chr2:10780250-10780269 | None:intergenic | 40.0% | |
| ATGGAAGAAGTAGCATTTCC+GGG | + | Chr2:10780399-10780418 | None:intergenic | 40.0% | |
| ATTCAAAGGTGAAGGGTTCA+AGG | - | Chr2:10780323-10780342 | MsG0280007110.01.T01:intron | 40.0% | |
| GCCACAACCTGAATCAAATT+TGG | + | Chr2:10780110-10780129 | None:intergenic | 40.0% | |
| GGATTACATGTGCAGATTGA+AGG | - | Chr2:10780003-10780022 | MsG0280007110.01.T01:CDS | 40.0% | |
| GGTCATGTATTCAAAGGTGA+AGG | - | Chr2:10780315-10780334 | MsG0280007110.01.T01:intron | 40.0% | |
| TCGAGAAATCTGAGGAAGAA+TGG | - | Chr2:10779685-10779704 | MsG0280007110.01.T01:three_prime_UTR | 40.0% | |
| !! | AATTTGATTCAGGTTGTGGC+TGG | - | Chr2:10780110-10780129 | MsG0280007110.01.T01:intron | 40.0% |
| !! | AGCTTGTAGTCAGTCTTGTT+TGG | - | Chr2:10780474-10780493 | MsG0280007110.01.T01:CDS | 40.0% |
| !! | TTGTAGTCAGTCTTGTTTGG+TGG | - | Chr2:10780477-10780496 | MsG0280007110.01.T01:CDS | 40.0% |
| CATCTGGATCAGGCTGAAAA+TGG | + | Chr2:10780251-10780270 | None:intergenic | 45.0% | |
| TCAGTCTAAACTGTGCTCAG+TGG | + | Chr2:10780533-10780552 | None:intergenic | 45.0% | |
| TTTCACATCCTTCGCCAGAA+AGG | - | Chr2:10779732-10779751 | MsG0280007110.01.T01:CDS | 45.0% | |
| ! | ATAACTTGTGCAGCATGTGG+TGG | - | Chr2:10780285-10780304 | MsG0280007110.01.T01:intron | 45.0% |
| ! | ATGTGCAGATTGAAGGGTAC+TGG | - | Chr2:10780010-10780029 | MsG0280007110.01.T01:CDS | 45.0% |
| ! | CATTTTCAGCCTGATCCAGA+TGG | - | Chr2:10780249-10780268 | MsG0280007110.01.T01:intron | 45.0% |
| ! | GAGATAACTTGTGCAGCATG+TGG | - | Chr2:10780282-10780301 | MsG0280007110.01.T01:intron | 45.0% |
| ! | TAACTTGTGCAGCATGTGGT+GGG | - | Chr2:10780286-10780305 | MsG0280007110.01.T01:intron | 45.0% |
| ATCTCAGTCCTCCTACCATC+TGG | + | Chr2:10780267-10780286 | None:intergenic | 50.0% | |
| CAAGCAAGTGTGCGTGCAAA+AGG | + | Chr2:10779623-10779642 | None:intergenic | 50.0% | |
| CGAAGGATGTGAAACTGCTC+AGG | + | Chr2:10779726-10779745 | None:intergenic | 50.0% | |
| GGGAAACCCTCAAAGAAAGC+AGG | + | Chr2:10780136-10780155 | None:intergenic | 50.0% | |
| TTCAGCCTGATCCAGATGGT+AGG | - | Chr2:10780253-10780272 | MsG0280007110.01.T01:intron | 50.0% | |
| ! | ACAGAGCGATTGATAGCTCC+AGG | + | Chr2:10780157-10780176 | None:intergenic | 50.0% |
| ! | ACTCAGTTCCTTTCTGGCGA+AGG | + | Chr2:10779743-10779762 | None:intergenic | 50.0% |
| ! | CAGAGCGATTGATAGCTCCA+GGG | + | Chr2:10780156-10780175 | None:intergenic | 50.0% |
| ! | GCTTTCTTTGAGGGTTTCCC+TGG | - | Chr2:10780136-10780155 | MsG0280007110.01.T01:intron | 50.0% |
| AGCCTGATCCAGATGGTAGG+AGG | - | Chr2:10780256-10780275 | MsG0280007110.01.T01:intron | 55.0% | |
| GTCCTCCTACCATCTGGATC+AGG | + | Chr2:10780261-10780280 | None:intergenic | 55.0% | |
| !! | GGCTGGCCTGCTTTCTTTGA+GGG | - | Chr2:10780127-10780146 | MsG0280007110.01.T01:intron | 55.0% |
| !! | TGGCTGGCCTGCTTTCTTTG+AGG | - | Chr2:10780126-10780145 | MsG0280007110.01.T01:intron | 55.0% |
| !! | GCAGCATGTGGTGGGCACTT+AGG | - | Chr2:10780294-10780313 | MsG0280007110.01.T01:intron | 60.0% |
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| Chr2 | gene | 10779560 | 10780569 | 10779560 | ID=MsG0280007110.01;Name=MsG0280007110.01 |
| Chr2 | mRNA | 10779560 | 10780569 | 10779560 | ID=MsG0280007110.01.T01;Parent=MsG0280007110.01;Name=MsG0280007110.01.T01;_AED=0.40;_eAED=0.40;_QI=34|1|1|1|1|1|3|147|162 |
| Chr2 | exon | 10779560 | 10779871 | 10779560 | ID=MsG0280007110.01.T01:exon:2561;Parent=MsG0280007110.01.T01 |
| Chr2 | exon | 10779955 | 10780111 | 10779955 | ID=MsG0280007110.01.T01:exon:2560;Parent=MsG0280007110.01.T01 |
| Chr2 | exon | 10780369 | 10780569 | 10780369 | ID=MsG0280007110.01.T01:exon:2559;Parent=MsG0280007110.01.T01 |
| Chr2 | five_prime_UTR | 10780536 | 10780569 | 10780536 | ID=MsG0280007110.01.T01:five_prime_utr;Parent=MsG0280007110.01.T01 |
| Chr2 | CDS | 10780369 | 10780535 | 10780369 | ID=MsG0280007110.01.T01:cds;Parent=MsG0280007110.01.T01 |
| Chr2 | CDS | 10779955 | 10780111 | 10779955 | ID=MsG0280007110.01.T01:cds;Parent=MsG0280007110.01.T01 |
| Chr2 | CDS | 10779707 | 10779871 | 10779707 | ID=MsG0280007110.01.T01:cds;Parent=MsG0280007110.01.T01 |
| Chr2 | three_prime_UTR | 10779560 | 10779706 | 10779560 | ID=MsG0280007110.01.T01:three_prime_utr;Parent=MsG0280007110.01.T01 |
| Gene Sequence |
| Protein sequence |
