Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MsG0280007155.01.T01 | AES64365.2 | 72.222 | 90 | 25 | 0 | 1 | 90 | 1 | 90 | 4.06E-37 | 141 |
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MsG0280007155.01.T01 | G7IKF9 | 72.222 | 90 | 25 | 0 | 1 | 90 | 1 | 90 | 1.94e-37 | 141 |
| Gene ID | Type | Classification |
|---|
| Gene ID | Type | Classification |
|---|
Co-expression Network
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|---|---|---|---|
| MsG0280007155.01 | MsG0380014930.01 | 0.804814 | 1.803539e-49 | 9.390279e-47 |
| MsG0280007155.01 | MsG0780041559.01 | 0.801398 | 9.175035e-49 | 4.377090e-46 |
| MsG0280007155.01 | MsG0880046933.01 | 0.805044 | 1.614118e-49 | 8.453533e-47 |
| MsG0280007155.01 | MsG0880046973.01 | 0.823528 | 1.319670e-53 | 1.139165e-50 |
PPI
| Gene1 | Gene2 | Type |
|---|
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MsG0280007155.01.T01 | MTR_2g021560 | 72.222 | 90 | 25 | 0 | 1 | 90 | 1 | 90 | 4.91e-41 | 141 |
| MsG0280007155.01.T01 | MTR_2g021630 | 36.792 | 212 | 81 | 3 | 1 | 212 | 1 | 159 | 3.01e-31 | 113 |
| MsG0280007155.01.T01 | MTR_2g020500 | 40.123 | 162 | 75 | 2 | 1 | 146 | 12 | 167 | 2.03e-30 | 112 |
| MsG0280007155.01.T01 | MTR_2g021510 | 35.981 | 214 | 82 | 4 | 1 | 212 | 11 | 171 | 1.06e-29 | 109 |
| MsG0280007155.01.T01 | MTR_2g021480 | 36.967 | 211 | 112 | 3 | 1 | 206 | 12 | 206 | 2.60e-29 | 110 |
| MsG0280007155.01.T01 | MTR_2g021600 | 33.913 | 230 | 77 | 5 | 2 | 210 | 3 | 178 | 4.11e-28 | 107 |
| MsG0280007155.01.T01 | MTR_2g021530 | 63.014 | 73 | 27 | 0 | 6 | 78 | 2 | 74 | 1.29e-26 | 99.8 |
| MsG0280007155.01.T01 | MTR_3g087310 | 59.211 | 76 | 31 | 0 | 1 | 76 | 1 | 76 | 5.43e-26 | 96.7 |
| MsG0280007155.01.T01 | MTR_5g048270 | 33.043 | 230 | 128 | 4 | 6 | 212 | 2 | 228 | 6.88e-24 | 95.9 |
| MsG0280007155.01.T01 | MTR_1g048260 | 32.889 | 225 | 104 | 7 | 1 | 200 | 1 | 203 | 1.35e-22 | 92.0 |
| MsG0280007155.01.T01 | MTR_1g025830 | 48.936 | 94 | 39 | 2 | 1 | 94 | 1 | 85 | 2.78e-21 | 88.6 |
| MsG0280007155.01.T01 | MTR_2g021450 | 52.055 | 73 | 35 | 0 | 1 | 73 | 1 | 73 | 3.69e-21 | 86.3 |
| MsG0280007155.01.T01 | MTR_1g025810 | 48.936 | 94 | 39 | 2 | 1 | 94 | 1 | 85 | 4.65e-21 | 88.6 |
| MsG0280007155.01.T01 | MTR_2g021570 | 62.500 | 56 | 21 | 0 | 6 | 61 | 28 | 83 | 5.06e-17 | 73.9 |
| MsG0280007155.01.T01 | MTR_2g021640 | 47.500 | 80 | 41 | 1 | 1 | 79 | 31 | 110 | 1.43e-14 | 70.1 |
| MsG0280007155.01.T01 | MTR_2g022750 | 55.556 | 54 | 24 | 0 | 8 | 61 | 11 | 64 | 3.62e-13 | 66.2 |
| MsG0280007155.01.T01 | MTR_2g022750 | 55.556 | 54 | 24 | 0 | 8 | 61 | 11 | 64 | 4.76e-13 | 65.9 |
| MsG0280007155.01.T01 | MTR_1g025850 | 31.507 | 219 | 110 | 7 | 1 | 210 | 15 | 202 | 9.10e-13 | 65.5 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|
Find 41 sgRNAs with CRISPR-Local
Find 42 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| TGATGATCGAAATCCTATTT+AGG | 0.141736 | 2:-11235763 | MsG0280007155.01.T01:CDS |
| GCAACAGCAGCAGAAGAAAA+AGG | 0.267790 | 2:-11235530 | MsG0280007155.01.T01:CDS |
| GATGATCGAAATCCTATTTA+GGG | 0.289195 | 2:-11235762 | MsG0280007155.01.T01:CDS |
| AGTTTCTCAGTTGCATAATC+TGG | 0.318379 | 2:-11235411 | MsG0280007155.01.T01:CDS |
| AGTTGCTGAGTTGGATGATT+TGG | 0.351868 | 2:-11235444 | MsG0280007155.01.T01:CDS |
| TGCTCAAATCAACCCTAAAT+AGG | 0.353861 | 2:+11235750 | None:intergenic |
| GGCAGCTCAGTTGGAGGAGC+AGG | 0.378310 | 2:-11235336 | MsG0280007155.01.T01:CDS |
| GGCAATTCAGTTGGAGGAGC+AGG | 0.408968 | 2:-11235357 | MsG0280007155.01.T01:CDS |
| ACTTAATTTGCTAGCTCAGT+TGG | 0.422432 | 2:-11235246 | MsG0280007155.01.T01:CDS |
| GGAGCAGGCAGCAGCTCAGT+TGG | 0.465294 | 2:-11235321 | MsG0280007155.01.T01:CDS |
| CAATATCAAAGTTGCTCAGT+TGG | 0.467218 | 2:-11235387 | MsG0280007155.01.T01:CDS |
| GGAGGAGCAGGCAGCTCAGT+TGG | 0.472256 | 2:-11235345 | MsG0280007155.01.T01:CDS |
| GTCACCGAGCACCGTACTAC+CGG | 0.476154 | 2:-11235792 | MsG0280007155.01.T01:CDS |
| TGTTGTTCTTGAAGCGTGAT+GGG | 0.481863 | 2:+11235563 | None:intergenic |
| CATCAGTTCACCCGGTAGTA+CGG | 0.482833 | 2:+11235781 | None:intergenic |
| TGAGCAATCCTCTGCAATTG+AGG | 0.486873 | 2:-11235733 | MsG0280007155.01.T01:CDS |
| ATGTTGTTCTTGAAGCGTGA+TGG | 0.496284 | 2:+11235562 | None:intergenic |
| TGCTGAGTTGGATGATTTGG+TGG | 0.505467 | 2:-11235441 | MsG0280007155.01.T01:CDS |
| GGCGCTGATGGCAGAAGCCA+CGG | 0.509560 | 2:-11235819 | None:intergenic |
| AGTTGCTCAGTTGGAGGAGG+AGG | 0.509992 | 2:-11235378 | MsG0280007155.01.T01:CDS |
| ACTTTGCATGAAGCTGCGAT+TGG | 0.514370 | 2:+11235588 | None:intergenic |
| GATGAAAATAGTTGCTGAGT+TGG | 0.517350 | 2:-11235453 | MsG0280007155.01.T01:CDS |
| GGAGGAGGAGGCAATTCAGT+TGG | 0.517966 | 2:-11235366 | MsG0280007155.01.T01:CDS |
| TGAGGTGCATTTGCAAGTTG+TGG | 0.525150 | 2:-11235715 | MsG0280007155.01.T01:CDS |
| AAGTGCTTCTTCATGAAAAG+AGG | 0.529207 | 2:+11235674 | None:intergenic |
| GGAGGAGGCAATTCAGTTGG+AGG | 0.558331 | 2:-11235363 | MsG0280007155.01.T01:CDS |
| TATCAAAGTTGCTCAGTTGG+AGG | 0.558827 | 2:-11235384 | MsG0280007155.01.T01:CDS |
| GGAGCAGGCAGCTCAGTTGG+AGG | 0.559133 | 2:-11235342 | MsG0280007155.01.T01:CDS |
| TGAGAGGATGAAGATGTTGG+TGG | 0.584272 | 2:+11235177 | None:intergenic |
| CAAAGTTGCTCAGTTGGAGG+AGG | 0.591958 | 2:-11235381 | MsG0280007155.01.T01:CDS |
| AGATCTACATGGTAAGTACA+AGG | 0.594387 | 2:-11235627 | MsG0280007155.01.T01:CDS |
| AATTGAGAGGATGAAGATGT+TGG | 0.604397 | 2:+11235174 | None:intergenic |
| TCACCGAGCACCGTACTACC+GGG | 0.616217 | 2:-11235791 | MsG0280007155.01.T01:CDS |
| ATTTCGATCATCAGTTCACC+CGG | 0.622076 | 2:+11235773 | None:intergenic |
| AATTGATAATCATAATTGAG+AGG | 0.622097 | 2:+11235161 | None:intergenic |
| CAAATGCACCTCAATTGCAG+AGG | 0.622136 | 2:+11235725 | None:intergenic |
| TCACCCGGTAGTACGGTGCT+CGG | 0.642487 | 2:+11235788 | None:intergenic |
| ACTGAAATCAAAGATCTACA+TGG | 0.649355 | 2:-11235638 | MsG0280007155.01.T01:CDS |
| GTTGTTCTTGAAGCGTGATG+GGG | 0.666706 | 2:+11235564 | None:intergenic |
| GGTGCTCGGTGACTCCGCCG+TGG | 0.682002 | 2:+11235802 | None:intergenic |
| GCTGATGGCAGAAGCCACGG+CGG | 0.743517 | 2:-11235816 | None:intergenic |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|---|---|---|---|---|
| !!! | AATTGATAATCATAATTGAG+AGG | + | Chr2:11235805-11235824 | None:intergenic | 20.0% |
| ACTGAAATCAAAGATCTACA+TGG | - | Chr2:11235325-11235344 | MsG0280007155.01.T01:CDS | 30.0% | |
| GATGATCGAAATCCTATTTA+GGG | - | Chr2:11235201-11235220 | MsG0280007155.01.T01:CDS | 30.0% | |
| TGATGATCGAAATCCTATTT+AGG | - | Chr2:11235200-11235219 | MsG0280007155.01.T01:CDS | 30.0% | |
| !! | TCAGTTGGATAATGTTTTAG+CGG | - | Chr2:11235657-11235676 | MsG0280007155.01.T01:CDS | 30.0% |
| !!! | ATAATGTTTTAGCGGTTGTT+AGG | - | Chr2:11235665-11235684 | MsG0280007155.01.T01:CDS | 30.0% |
| AAGTGCTTCTTCATGAAAAG+AGG | + | Chr2:11235292-11235311 | None:intergenic | 35.0% | |
| AATTGAGAGGATGAAGATGT+TGG | + | Chr2:11235792-11235811 | None:intergenic | 35.0% | |
| AGATCTACATGGTAAGTACA+AGG | - | Chr2:11235336-11235355 | MsG0280007155.01.T01:CDS | 35.0% | |
| AGTTTCTCAGTTGCATAATC+TGG | - | Chr2:11235552-11235571 | MsG0280007155.01.T01:CDS | 35.0% | |
| CAATATCAAAGTTGCTCAGT+TGG | - | Chr2:11235576-11235595 | MsG0280007155.01.T01:CDS | 35.0% | |
| GATGAAAATAGTTGCTGAGT+TGG | - | Chr2:11235510-11235529 | MsG0280007155.01.T01:CDS | 35.0% | |
| TGCTCAAATCAACCCTAAAT+AGG | + | Chr2:11235216-11235235 | None:intergenic | 35.0% | |
| !! | ACTTAATTTGCTAGCTCAGT+TGG | - | Chr2:11235717-11235736 | MsG0280007155.01.T01:CDS | 35.0% |
| ATGTTGTTCTTGAAGCGTGA+TGG | + | Chr2:11235404-11235423 | None:intergenic | 40.0% | |
| ATTTCGATCATCAGTTCACC+CGG | + | Chr2:11235193-11235212 | None:intergenic | 40.0% | |
| TATCAAAGTTGCTCAGTTGG+AGG | - | Chr2:11235579-11235598 | MsG0280007155.01.T01:CDS | 40.0% | |
| TGTTGTTCTTGAAGCGTGAT+GGG | + | Chr2:11235403-11235422 | None:intergenic | 40.0% | |
| ! | AGTTGCTGAGTTGGATGATT+TGG | - | Chr2:11235519-11235538 | MsG0280007155.01.T01:CDS | 40.0% |
| ! | GTGTCTCAAAAGCTTTTTGC+AGG | - | Chr2:11235750-11235769 | MsG0280007155.01.T01:CDS | 40.0% |
| ACTTTGCATGAAGCTGCGAT+TGG | + | Chr2:11235378-11235397 | None:intergenic | 45.0% | |
| CAAATGCACCTCAATTGCAG+AGG | + | Chr2:11235241-11235260 | None:intergenic | 45.0% | |
| GCAACAGCAGCAGAAGAAAA+AGG | - | Chr2:11235433-11235452 | MsG0280007155.01.T01:CDS | 45.0% | |
| GTTGTTCTTGAAGCGTGATG+GGG | + | Chr2:11235402-11235421 | None:intergenic | 45.0% | |
| TGAGAGGATGAAGATGTTGG+TGG | + | Chr2:11235789-11235808 | None:intergenic | 45.0% | |
| TGAGCAATCCTCTGCAATTG+AGG | - | Chr2:11235230-11235249 | MsG0280007155.01.T01:CDS | 45.0% | |
| TGAGGTGCATTTGCAAGTTG+TGG | - | Chr2:11235248-11235267 | MsG0280007155.01.T01:CDS | 45.0% | |
| ! | TGCTGAGTTGGATGATTTGG+TGG | - | Chr2:11235522-11235541 | MsG0280007155.01.T01:CDS | 45.0% |
| CAAAGTTGCTCAGTTGGAGG+AGG | - | Chr2:11235582-11235601 | MsG0280007155.01.T01:CDS | 50.0% | |
| CATCAGTTCACCCGGTAGTA+CGG | + | Chr2:11235185-11235204 | None:intergenic | 50.0% | |
| AGTTGCTCAGTTGGAGGAGG+AGG | - | Chr2:11235585-11235604 | MsG0280007155.01.T01:CDS | 55.0% | |
| GGAGGAGGAGGCAATTCAGT+TGG | - | Chr2:11235597-11235616 | MsG0280007155.01.T01:CDS | 55.0% | |
| GGAGGAGGCAATTCAGTTGG+AGG | - | Chr2:11235600-11235619 | MsG0280007155.01.T01:CDS | 55.0% | |
| GGCAATTCAGTTGGAGGAGC+AGG | - | Chr2:11235606-11235625 | MsG0280007155.01.T01:CDS | 55.0% | |
| TCACCCGGTAGTACGGTGCT+CGG | + | Chr2:11235178-11235197 | None:intergenic | 60.0% | |
| ! | GTCACCGAGCACCGTACTAC+CGG | - | Chr2:11235171-11235190 | MsG0280007155.01.T01:CDS | 60.0% |
| ! | TCACCGAGCACCGTACTACC+GGG | - | Chr2:11235172-11235191 | MsG0280007155.01.T01:CDS | 60.0% |
| GGAGCAGGCAGCAGCTCAGT+TGG | - | Chr2:11235642-11235661 | MsG0280007155.01.T01:CDS | 65.0% | |
| GGAGCAGGCAGCTCAGTTGG+AGG | - | Chr2:11235621-11235640 | MsG0280007155.01.T01:CDS | 65.0% | |
| GGAGGAGCAGGCAGCTCAGT+TGG | - | Chr2:11235618-11235637 | MsG0280007155.01.T01:CDS | 65.0% | |
| GGCAGCTCAGTTGGAGGAGC+AGG | - | Chr2:11235627-11235646 | MsG0280007155.01.T01:CDS | 65.0% | |
| ! | GGTGCTCGGTGACTCCGCCG+TGG | + | Chr2:11235164-11235183 | None:intergenic | 75.0% |
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| Chr2 | gene | 11235151 | 11235834 | 11235151 | ID=MsG0280007155.01;Name=MsG0280007155.01 |
| Chr2 | mRNA | 11235151 | 11235834 | 11235151 | ID=MsG0280007155.01.T01;Parent=MsG0280007155.01;Name=MsG0280007155.01.T01;_AED=0.49;_eAED=0.49;_QI=0|-1|0|1|-1|1|1|0|227 |
| Chr2 | exon | 11235151 | 11235834 | 11235151 | ID=MsG0280007155.01.T01:exon:312;Parent=MsG0280007155.01.T01 |
| Chr2 | CDS | 11235151 | 11235834 | 11235151 | ID=MsG0280007155.01.T01:cds;Parent=MsG0280007155.01.T01 |
| Gene Sequence |
| Protein sequence |
