AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar ZhongmuNo.1 / MsG0280007223.01

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MsG0280007223.01.T01 MTR_2g022880 68.452 336 23 4 1 305 1 284 3.30e-136 387
MsG0280007223.01.T01 MTR_2g022880 78.027 223 10 3 1 186 1 221 2.38e-102 300
MsG0280007223.01.T01 MTR_4g131930 51.282 312 71 5 25 305 17 278 1.31e-98 291
MsG0280007223.01.T01 MTR_4g094212 42.683 328 105 6 11 305 12 289 7.67e-81 246
MsG0280007223.01.T01 MTR_1g033830 40.798 326 110 5 13 305 22 297 3.55e-73 227
MsG0280007223.01.T01 MTR_4g131930 48.864 264 54 5 73 305 2 215 5.69e-73 224
MsG0280007223.01.T01 MTR_1g033830 44.554 202 79 2 13 181 22 223 3.66e-51 168
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MsG0280007223.01.T01 AT4G21790 50.156 321 77 5 18 305 21 291 4.49e-100 296
MsG0280007223.01.T01 AT2G02180 40.491 326 111 5 13 305 28 303 6.42e-76 234
MsG0280007223.01.T01 AT1G14530 39.349 338 122 5 1 305 6 293 3.43e-66 209
MsG0280007223.01.T01 AT1G14530 38.843 242 107 4 1 203 6 245 4.81e-51 169

Find 80 sgRNAs with CRISPR-Local

Find 215 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
GCAATAGGATTTCTAATATT+TGG 0.124625 2:+12153325 MsG0280007223.01.T01:CDS
CCTTCTCCGATTCATCTTCA+TGG 0.190874 2:+12151094 MsG0280007223.01.T01:CDS
CCAAGATGCTCTACAGTTAA+TGG 0.201166 2:-12154434 None:intergenic
CTTCTCCCCTTCGACTCAAT+TGG 0.225125 2:-12153513 None:intergenic
TTTGGATTCAGGTTGGTATT+TGG 0.238716 2:+12153099 MsG0280007223.01.T01:intron
TTTCACATCATGTAAGTAAA+AGG 0.248422 2:+12154647 MsG0280007223.01.T01:three_prime_UTR
GGATGCATCTCTTGATGTTT+TGG 0.250674 2:+12154197 MsG0280007223.01.T01:CDS
GCGGAAAGTGTTACAGAAAA+TGG 0.287187 2:+12151038 None:intergenic
TGAGTAGTCCAACCATATTC+AGG 0.302915 2:-12151231 None:intergenic
AATCCCATGAAATTTGAAAA+TGG 0.306371 2:-12154755 None:intergenic
TTCATTGGAAAGATATTTAT+AGG 0.306665 2:+12153158 MsG0280007223.01.T01:CDS
GAACTTAGAGTACCTGAATA+TGG 0.316772 2:+12151219 MsG0280007223.01.T01:CDS
ATAGTTTACATCTCTGTAAA+TGG 0.340354 2:+12152588 MsG0280007223.01.T01:CDS
TCAGCAGTTGCTGCTCGGTT+AGG 0.360171 2:+12154576 MsG0280007223.01.T01:three_prime_UTR
TTGAGATCGACATGCTCATT+GGG 0.368705 2:+12154023 MsG0280007223.01.T01:intron
TAGTAGATGAAGTCCAAAAT+AGG 0.369339 2:-12154225 None:intergenic
AATGATGTTGTGGACTTCAT+TGG 0.374030 2:+12153143 MsG0280007223.01.T01:CDS
TTTAATGGCGCTGCAGTTCA+TGG 0.375212 2:+12154490 MsG0280007223.01.T01:three_prime_UTR
TTCTCCCCTTCGACTCAATT+GGG 0.391195 2:-12153512 None:intergenic
GTATCATTTGTAGCTGCAAT+AGG 0.392978 2:+12153310 MsG0280007223.01.T01:CDS
GCAGTACGTGCTCTTGTGTT+TGG 0.396156 2:+12151395 MsG0280007223.01.T01:intron
TCTTGTATCTGCAGTTGATT+CGG 0.410575 2:+12154176 MsG0280007223.01.T01:CDS
CAGCAGTTGCTGCTCGGTTA+GGG 0.414023 2:+12154577 MsG0280007223.01.T01:three_prime_UTR
CATAGATCTTATCAGAAAAC+AGG 0.416104 2:-12153792 None:intergenic
TTGGTATTTGGATTTACCTC+TGG 0.418748 2:+12153111 MsG0280007223.01.T01:CDS
GTAAAAGGAGCAAGGATTGA+GGG 0.423945 2:+12154662 MsG0280007223.01.T01:three_prime_UTR
ATGTGTAAATTACAAATAGA+TGG 0.428860 2:+12154782 MsG0280007223.01.T01:three_prime_UTR
TATTGAGTTGATATTCTCTT+CGG 0.451424 2:-12154402 None:intergenic
ATTGAGATCGACATGCTCAT+TGG 0.461653 2:+12154022 MsG0280007223.01.T01:intron
CTGCTTGAGAATATACTTGA+TGG 0.463844 2:-12154531 None:intergenic
CCATTAACTGTAGAGCATCT+TGG 0.464462 2:+12154434 MsG0280007223.01.T01:exon
CGACATGCTCATTGGGGAGG+GGG 0.473984 2:+12154030 MsG0280007223.01.T01:CDS
CACAGAGAGTGTAGAAAAGT+GGG 0.475634 2:-12151146 None:intergenic
GTGAAACCATGAAGATGAAT+CGG 0.477113 2:-12151100 None:intergenic
CTTAGTACTTGTCAATTGTC+CGG 0.494243 2:+12154054 MsG0280007223.01.T01:CDS
GAGTTGAATAAGAGCAATGA+AGG 0.494346 2:-12151187 None:intergenic
ATCGACATGCTCATTGGGGA+GGG 0.498183 2:+12154028 MsG0280007223.01.T01:CDS
GATTTCTAATATTTGGAGGA+AGG 0.500607 2:+12153332 MsG0280007223.01.T01:CDS
GTAGAAAAGTGGGGAGTGAG+TGG 0.511615 2:-12151136 None:intergenic
AGTAAAAGGAGCAAGGATTG+AGG 0.513487 2:+12154661 MsG0280007223.01.T01:three_prime_UTR
GCACAGAGAGTGTAGAAAAG+TGG 0.523675 2:-12151147 None:intergenic
GATTGAGGGAAGTTCATTGA+GGG 0.528468 2:+12154676 MsG0280007223.01.T01:three_prime_UTR
TGCTGATTATCACACATGTA+AGG 0.530767 2:-12154559 None:intergenic
GCGTTTCCCAATTGAGTCGA+AGG 0.541700 2:+12153506 MsG0280007223.01.T01:CDS
TCTTCGGTGGCAATTTGCGT+AGG 0.542095 2:-12154386 None:intergenic
TTTGGACTTCATCTACTACA+TGG 0.542851 2:+12154230 MsG0280007223.01.T01:CDS
TGAGTTGATATTCTCTTCGG+TGG 0.546195 2:-12154399 None:intergenic
GTTTACCAACTGATAAGCTC+AGG 0.551142 2:+12152565 MsG0280007223.01.T01:CDS
ATAGGATTTCTAATATTTGG+AGG 0.559894 2:+12153328 MsG0280007223.01.T01:CDS
TGGTGCTGTCTATTTAATCC+AGG 0.560182 2:+12152608 MsG0280007223.01.T01:CDS
TTAGAGTACCTGAATATGGT+TGG 0.563767 2:+12151223 MsG0280007223.01.T01:CDS
CCATGAAGATGAATCGGAGA+AGG 0.564612 2:-12151094 None:intergenic
GGATTGAGGGAAGTTCATTG+AGG 0.565409 2:+12154675 MsG0280007223.01.T01:three_prime_UTR
CGTTTCCCAATTGAGTCGAA+GGG 0.580317 2:+12153507 MsG0280007223.01.T01:CDS
GATCGACATGCTCATTGGGG+AGG 0.587206 2:+12154027 MsG0280007223.01.T01:intron
GCCATTAAAATAATAACCGA+CGG 0.588828 2:-12154476 None:intergenic
ATTGGAAAGATATTTATAGG+AGG 0.589271 2:+12153161 MsG0280007223.01.T01:CDS
GATAATCAGCAGTTGCTGCT+CGG 0.591290 2:+12154571 MsG0280007223.01.T01:three_prime_UTR
GCAATTTGCGTAGGATGAAG+AGG 0.595166 2:-12154377 None:intergenic
TCGACATGCTCATTGGGGAG+GGG 0.602610 2:+12154029 MsG0280007223.01.T01:CDS
CCATTAAAATAATAACCGAC+GGG 0.604946 2:-12154475 None:intergenic
GTTAGATTACAATGATGTTG+TGG 0.605926 2:+12153133 MsG0280007223.01.T01:CDS
TTACTGTATTCTTCACCCGT+CGG 0.609725 2:+12154460 MsG0280007223.01.T01:three_prime_UTR
AGAGGACTATAGCTGAAGGC+AGG 0.610867 2:-12154359 None:intergenic
TGCTTGAGAATATACTTGAT+GGG 0.615437 2:-12154530 None:intergenic
TCATGTAAGTAAAAGGAGCA+AGG 0.617131 2:+12154654 MsG0280007223.01.T01:three_prime_UTR
AGATGAAGTCCAAAATAGGA+TGG 0.617784 2:-12154221 None:intergenic
TGTAAATTACAAATAGATGG+AGG 0.623062 2:+12154785 MsG0280007223.01.T01:three_prime_UTR
TGAGATCGACATGCTCATTG+GGG 0.632813 2:+12154024 MsG0280007223.01.T01:intron
AAATTGCAAACAAAATCTCA+AGG 0.635890 2:+12154079 MsG0280007223.01.T01:CDS
ATGAAGAGGACTATAGCTGA+AGG 0.637421 2:-12154363 None:intergenic
GATTAAATTTAATATATGTG+AGG 0.637564 2:+12154101 MsG0280007223.01.T01:CDS
ACTATCCTGAGCTTATCAGT+TGG 0.646966 2:-12152570 None:intergenic
GAGAAGAAAGAAGCTGCAAG+AGG 0.662827 2:+12153530 MsG0280007223.01.T01:CDS
ATATACTTGATGGGCAAGAG+CGG 0.663347 2:-12154521 None:intergenic
ATTGAGGGAAGTTCATTGAG+GGG 0.671328 2:+12154677 MsG0280007223.01.T01:three_prime_UTR
GTTTCCCAATTGAGTCGAAG+GGG 0.679602 2:+12153508 MsG0280007223.01.T01:CDS
CATCATTGTAATCTAACCAG+AGG 0.684919 2:-12153127 None:intergenic
ACAGAGAGTGTAGAAAAGTG+GGG 0.694273 2:-12151145 None:intergenic
AGTGAGTGGAATCTGTGACA+AGG 0.749614 2:-12151122 None:intergenic

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!!! TTTTTATATATATATATATT+TGG + Chr2:12151361-12151380 MsG0280007223.01.T01:intron 0.0%
!!! AATGAATTTTATAAGAAAAA+CGG + Chr2:12151676-12151695 MsG0280007223.01.T01:intron 10.0%
!! GATTAAATTTAATATATGTG+AGG + Chr2:12154101-12154120 MsG0280007223.01.T01:CDS 15.0%
!! TTATGTTTATATACAATTCA+CGG + Chr2:12151590-12151609 MsG0280007223.01.T01:intron 15.0%
!!! ATGAATTTTATAAGAAAAAC+GGG + Chr2:12151677-12151696 MsG0280007223.01.T01:intron 15.0%
!!! GTTATTTTTGTAAATATGTT+GGG + Chr2:12153880-12153899 MsG0280007223.01.T01:intron 15.0%
!!! GTTTAAAGTAAAATGATTTA+TGG + Chr2:12151914-12151933 MsG0280007223.01.T01:intron 15.0%
!!! TATAGAAATTTTTGTGATAT+AGG + Chr2:12154708-12154727 MsG0280007223.01.T01:three_prime_UTR 15.0%
!! AAAGTAAAATGATTTATGGT+TGG + Chr2:12151918-12151937 MsG0280007223.01.T01:intron 20.0%
!! AATGTATAATTATCTACATC+TGG - Chr2:12153684-12153703 None:intergenic 20.0%
!! ATGTGTAAATTACAAATAGA+TGG + Chr2:12154782-12154801 MsG0280007223.01.T01:three_prime_UTR 20.0%
!! TATACCAATGTAATGTTTAA+AGG - Chr2:12153244-12153263 None:intergenic 20.0%
!! TATGTTTATATACAATTCAC+GGG + Chr2:12151591-12151610 MsG0280007223.01.T01:intron 20.0%
!! TTCATTGGAAAGATATTTAT+AGG + Chr2:12153158-12153177 MsG0280007223.01.T01:CDS 20.0%
!!! AATTTTTTTTTATTTCCTCC+CGG + Chr2:12153449-12153468 MsG0280007223.01.T01:intron 20.0%
!!! ATTGTTTTTCATTTTTTGCT+TGG + Chr2:12153909-12153928 MsG0280007223.01.T01:intron 20.0%
!!! CACAAATAAACTAGTTTTAA+AGG - Chr2:12152417-12152436 None:intergenic 20.0%
!!! CGTTATTTTTGTAAATATGT+TGG + Chr2:12153879-12153898 MsG0280007223.01.T01:intron 20.0%
!!! TGAATTTTATAAGAAAAACG+GGG + Chr2:12151678-12151697 MsG0280007223.01.T01:intron 20.0%
!!! TTTCCATTTTCAAATTTCAT+GGG + Chr2:12154752-12154771 MsG0280007223.01.T01:three_prime_UTR 20.0%
! AAACTAGAATTTGTAACCTT+TGG - Chr2:12151649-12151668 None:intergenic 25.0%
! AAATTGCAAACAAAATCTCA+AGG + Chr2:12154079-12154098 MsG0280007223.01.T01:CDS 25.0%
! AATCCCATGAAATTTGAAAA+TGG - Chr2:12154758-12154777 None:intergenic 25.0%
! AGTTTGATAAAACTTTCTGA+GGG + Chr2:12153581-12153600 MsG0280007223.01.T01:intron 25.0%
! ATAGGATTTCTAATATTTGG+AGG + Chr2:12153328-12153347 MsG0280007223.01.T01:CDS 25.0%
! ATAGTTTACATCTCTGTAAA+TGG + Chr2:12152588-12152607 MsG0280007223.01.T01:CDS 25.0%
! ATTATGGCACAAAAATTTCT+AGG + Chr2:12152889-12152908 MsG0280007223.01.T01:intron 25.0%
! ATTGGAAAGATATTTATAGG+AGG + Chr2:12153161-12153180 MsG0280007223.01.T01:CDS 25.0%
! GCAATAGGATTTCTAATATT+TGG + Chr2:12153325-12153344 MsG0280007223.01.T01:CDS 25.0%
! TAGTTATACTCACATAAACT+CGG + Chr2:12153728-12153747 MsG0280007223.01.T01:intron 25.0%
! TGTAAATTACAAATAGATGG+AGG + Chr2:12154785-12154804 MsG0280007223.01.T01:three_prime_UTR 25.0%
! TTTCACATCATGTAAGTAAA+AGG + Chr2:12154647-12154666 MsG0280007223.01.T01:three_prime_UTR 25.0%
!! ATGGAATTCAATATCTGTTT+TGG + Chr2:12152973-12152992 MsG0280007223.01.T01:intron 25.0%
!! CTTTCCATTTTCAAATTTCA+TGG + Chr2:12154751-12154770 MsG0280007223.01.T01:three_prime_UTR 25.0%
!! GAATTTTATAAGAAAAACGG+GGG + Chr2:12151679-12151698 MsG0280007223.01.T01:intron 25.0%
!! TGGAATTCAATATCTGTTTT+GGG + Chr2:12152974-12152993 MsG0280007223.01.T01:intron 25.0%
!! TTGATTTGACTCTCTTAAAA+GGG + Chr2:12152112-12152131 MsG0280007223.01.T01:intron 25.0%
!! TTTTCTGATAAGATCTATGA+TGG + Chr2:12153796-12153815 MsG0280007223.01.T01:CDS 25.0%
!!! AATCTACTTTACTTTTCTTC+AGG + Chr2:12153286-12153305 MsG0280007223.01.T01:intron 25.0%
!!! ACTTAAAAGTGTCAAATTTG+CGG - Chr2:12152845-12152864 None:intergenic 25.0%
!!! GTTCTTTTTATTTCACAATG+AGG - Chr2:12153636-12153655 None:intergenic 25.0%
!!! TAAACTAGTTTTAAAGGACA+AGG - Chr2:12152411-12152430 None:intergenic 25.0%
!!! TATTGAGTTGATATTCTCTT+CGG - Chr2:12154405-12154424 None:intergenic 25.0%
!!! TTTGATTTGACTCTCTTAAA+AGG + Chr2:12152111-12152130 MsG0280007223.01.T01:intron 25.0%
!!! TTTTTATTTCATGATTGAGC+AGG + Chr2:12152536-12152555 MsG0280007223.01.T01:intron 25.0%
AAAATTTCTTCATCACACGA+AGG - Chr2:12152484-12152503 None:intergenic 30.0%
AAGGGATATTCATCATGTAT+TGG - Chr2:12152724-12152743 None:intergenic 30.0%
AGTATCACAATACTAAACTG+TGG + Chr2:12153075-12153094 MsG0280007223.01.T01:intron 30.0%
ATGTATTGGAATAAACACCA+AGG - Chr2:12152710-12152729 None:intergenic 30.0%
CAAAAGTGAAAACTAACCTT+AGG - Chr2:12151449-12151468 None:intergenic 30.0%
CAAAATTGAGGAATTGCAAA+AGG - Chr2:12153395-12153414 None:intergenic 30.0%
CAGTTTGATAAAACTTTCTG+AGG + Chr2:12153580-12153599 MsG0280007223.01.T01:intron 30.0%
CATAGATCTTATCAGAAAAC+AGG - Chr2:12153795-12153814 None:intergenic 30.0%
CATGAAGGAGAAAATACTAA+AGG + Chr2:12151766-12151785 MsG0280007223.01.T01:intron 30.0%
CCAACACTATCAATTCATAT+TGG - Chr2:12152937-12152956 None:intergenic 30.0%
CCACTGAATAAACAAAATTG+AGG - Chr2:12153407-12153426 None:intergenic 30.0%
CCATTAAAATAATAACCGAC+GGG - Chr2:12154478-12154497 None:intergenic 30.0%
CTTCAGCATGAAAAATAATC+TGG - Chr2:12153487-12153506 None:intergenic 30.0%
GATTTCTAATATTTGGAGGA+AGG + Chr2:12153332-12153351 MsG0280007223.01.T01:CDS 30.0%
GCAACCTTTAAACATTACAT+TGG + Chr2:12153237-12153256 MsG0280007223.01.T01:intron 30.0%
GCCATTAAAATAATAACCGA+CGG - Chr2:12154479-12154498 None:intergenic 30.0%
GTTAGATTACAATGATGTTG+TGG + Chr2:12153133-12153152 MsG0280007223.01.T01:CDS 30.0%
TAGATTGCAAGAATGTAGTT+AGG - Chr2:12152769-12152788 None:intergenic 30.0%
TAGATTTGAGTGTAAACCAA+AGG + Chr2:12151630-12151649 MsG0280007223.01.T01:intron 30.0%
TAGTAGATGAAGTCCAAAAT+AGG - Chr2:12154228-12154247 None:intergenic 30.0%
TCTGGTGTATGAAATGATTA+TGG + Chr2:12152873-12152892 MsG0280007223.01.T01:intron 30.0%
TGCTTGAGAATATACTTGAT+GGG - Chr2:12154533-12154552 None:intergenic 30.0%
TGGTTTACACTCAAATCTAT+TGG - Chr2:12151629-12151648 None:intergenic 30.0%
TTGAGTGTAAAAATCGTGTT+TGG + Chr2:12151975-12151994 MsG0280007223.01.T01:intron 30.0%
TTGCAAGTTGCTTCAATTAA+GGG - Chr2:12153993-12154012 None:intergenic 30.0%
! ACACCTTGTTTGAGTTAAAA+TGG + Chr2:12154830-12154849 MsG0280007223.01.T01:three_prime_UTR 30.0%
! ATACACTTCTTGTTCTTTTC+TGG + Chr2:12152348-12152367 MsG0280007223.01.T01:intron 30.0%
! ATTCGCTTCATTTTGTATGT+TGG + Chr2:12152680-12152699 MsG0280007223.01.T01:intron 30.0%
! GTTTGACTTTGAATATACTG+TGG + Chr2:12151852-12151871 MsG0280007223.01.T01:intron 30.0%
! TACACTTCTTGTTCTTTTCT+GGG + Chr2:12152349-12152368 MsG0280007223.01.T01:intron 30.0%
! TGAAGACTGCAAATTGATTT+CGG - Chr2:12152083-12152102 None:intergenic 30.0%
! TGGAAGTGGTATTTAAGATA+TGG + Chr2:12152954-12152973 MsG0280007223.01.T01:intron 30.0%
! TTACATTTTGTTACACATGC+AGG + Chr2:12154152-12154171 MsG0280007223.01.T01:CDS 30.0%
!! ACCTGTATTTTAAAAGCGAA+GGG - Chr2:12154330-12154349 None:intergenic 30.0%
!! ACTTTTAAGTGAAGATCTTC+TGG + Chr2:12152855-12152874 MsG0280007223.01.T01:intron 30.0%
!! CCAATATGAATTGATAGTGT+TGG + Chr2:12152934-12152953 MsG0280007223.01.T01:intron 30.0%
!! CCTCAATTTTGTTTATTCAG+TGG + Chr2:12153404-12153423 MsG0280007223.01.T01:intron 30.0%
!! GATTTTGTTTGCAATTTCTC+CGG - Chr2:12154076-12154095 None:intergenic 30.0%
!! TTTCATGAAAAAAGTGCCTA+TGG - Chr2:12153268-12153287 None:intergenic 30.0%
!!! CAATGGTTACTTTTTCATGT+AGG + Chr2:12152274-12152293 MsG0280007223.01.T01:intron 30.0%
!!! GTTCACTCTCTTTGTTTTTA+TGG + Chr2:12151297-12151316 MsG0280007223.01.T01:intron 30.0%
!!! TACTTCGTGTTCTTTTTTGT+TGG + Chr2:12154258-12154277 MsG0280007223.01.T01:intron 30.0%
!!! TTTCATGTAGGTTTTGACTT+TGG + Chr2:12152286-12152305 MsG0280007223.01.T01:intron 30.0%
AAAGCAAAAAAAGACATGCC+TGG - Chr2:12152388-12152407 None:intergenic 35.0%
AACTAAGGGTTTAAAGGAGT+TGG + Chr2:12151723-12151742 MsG0280007223.01.T01:intron 35.0%
AATCTGGAAACACGAATTAC+CGG - Chr2:12153471-12153490 None:intergenic 35.0%
AATGATGTTGTGGACTTCAT+TGG + Chr2:12153143-12153162 MsG0280007223.01.T01:CDS 35.0%
ACAATACTAAACTGTGGCTT+TGG + Chr2:12153081-12153100 MsG0280007223.01.T01:intron 35.0%
AGATGAAGTCCAAAATAGGA+TGG - Chr2:12154224-12154243 None:intergenic 35.0%
AGTCTGAACTAAGGGTTTAA+AGG + Chr2:12151717-12151736 MsG0280007223.01.T01:intron 35.0%
AGTGTAAAAATCGTGTTTGG+AGG + Chr2:12151978-12151997 MsG0280007223.01.T01:intron 35.0%
ATACTCACATAAACTCGGTT+TGG + Chr2:12153733-12153752 MsG0280007223.01.T01:intron 35.0%
CAAGAAATGAGATAGATACC+TGG - Chr2:12152629-12152648 None:intergenic 35.0%
CATCATTGTAATCTAACCAG+AGG - Chr2:12153130-12153149 None:intergenic 35.0%
CATTACATTGGTATAGCCAT+AGG + Chr2:12153249-12153268 MsG0280007223.01.T01:intron 35.0%
CTGCTTGAGAATATACTTGA+TGG - Chr2:12154534-12154553 None:intergenic 35.0%
CTTAGTACTTGTCAATTGTC+CGG + Chr2:12154054-12154073 MsG0280007223.01.T01:CDS 35.0%
CTTGCAAGTTGCTTCAATTA+AGG - Chr2:12153994-12154013 None:intergenic 35.0%
GAGTTGAATAAGAGCAATGA+AGG - Chr2:12151190-12151209 None:intergenic 35.0%
GATGAATATCCCTTTCAGTT+TGG + Chr2:12152730-12152749 MsG0280007223.01.T01:intron 35.0%
GGAGAAAATACTAAAGGAGT+TGG + Chr2:12151772-12151791 MsG0280007223.01.T01:intron 35.0%
GTATCATTTGTAGCTGCAAT+AGG + Chr2:12153310-12153329 MsG0280007223.01.T01:CDS 35.0%
GTGAAACCATGAAGATGAAT+CGG - Chr2:12151103-12151122 None:intergenic 35.0%
GTGAGAATTCTACAGTTGAT+TGG + Chr2:12153354-12153373 MsG0280007223.01.T01:intron 35.0%
GTTTATTCAGTGGTTGTTCT+TGG + Chr2:12153414-12153433 MsG0280007223.01.T01:intron 35.0%
TATAATCACTTTCCACTAGC+TGG - Chr2:12152459-12152478 None:intergenic 35.0%
TCATGTAAGTAAAAGGAGCA+AGG + Chr2:12154654-12154673 MsG0280007223.01.T01:three_prime_UTR 35.0%
TCTTGTATCTGCAGTTGATT+CGG + Chr2:12154176-12154195 MsG0280007223.01.T01:CDS 35.0%
TTATAAGAAAAACGGGGGTT+TGG + Chr2:12151684-12151703 MsG0280007223.01.T01:intron 35.0%
TTATGGTTGGATGTGTTTGT+AGG + Chr2:12151931-12151950 MsG0280007223.01.T01:intron 35.0%
TTGGTATTTGGATTTACCTC+TGG + Chr2:12153111-12153130 MsG0280007223.01.T01:CDS 35.0%
TTTGGACTTCATCTACTACA+TGG + Chr2:12154230-12154249 MsG0280007223.01.T01:CDS 35.0%
! AGTGTTTTTCTTGCATCCTA+AGG + Chr2:12151430-12151449 MsG0280007223.01.T01:CDS 35.0%
! ATACTTGACTTTTGTGTGTG+TGG + Chr2:12152170-12152189 MsG0280007223.01.T01:intron 35.0%
! GAACTTAGAGTACCTGAATA+TGG + Chr2:12151219-12151238 MsG0280007223.01.T01:CDS 35.0%
! TGCTGATTATCACACATGTA+AGG - Chr2:12154562-12154581 None:intergenic 35.0%
! TTAGAGTACCTGAATATGGT+TGG + Chr2:12151223-12151242 MsG0280007223.01.T01:CDS 35.0%
! TTCTGAGGGAAAAACATTCT+AGG + Chr2:12153595-12153614 MsG0280007223.01.T01:intron 35.0%
! TTTTGTATGTTGGATCTCCT+TGG + Chr2:12152690-12152709 MsG0280007223.01.T01:intron 35.0%
!! CACCTGTATTTTAAAAGCGA+AGG - Chr2:12154331-12154350 None:intergenic 35.0%
!! CTCTTCAACTTCGTTTTTAG+TGG + Chr2:12151267-12151286 MsG0280007223.01.T01:CDS 35.0%
!! TCACTTAGATTTGCTGAGAT+TGG + Chr2:12152219-12152238 MsG0280007223.01.T01:intron 35.0%
!! TGAATTGATAGTGTTGGAAG+TGG + Chr2:12152940-12152959 MsG0280007223.01.T01:intron 35.0%
!! TGTTTTGGACCATCCTATTT+TGG + Chr2:12154212-12154231 MsG0280007223.01.T01:CDS 35.0%
!! TTTGGATTCAGGTTGGTATT+TGG + Chr2:12153099-12153118 MsG0280007223.01.T01:intron 35.0%
!!! ACCCTTCGCTTTTAAAATAC+AGG + Chr2:12154326-12154345 MsG0280007223.01.T01:intron 35.0%
!!! CCCGTCGGTTATTATTTTAA+TGG + Chr2:12154475-12154494 MsG0280007223.01.T01:three_prime_UTR 35.0%
!!! CTTCGCTTTTAAAATACAGG+TGG + Chr2:12154329-12154348 MsG0280007223.01.T01:intron 35.0%
!!! TCTGTTTTGGGTTGTAATTG+TGG + Chr2:12152986-12153005 MsG0280007223.01.T01:intron 35.0%
!!! TTCAACTTCGTTTTTAGTGG+AGG + Chr2:12151270-12151289 MsG0280007223.01.T01:CDS 35.0%
AAAGGGAATTTGAACGTGCA+TGG + Chr2:12152129-12152148 MsG0280007223.01.T01:intron 40.0%
ACTATCCTGAGCTTATCAGT+TGG - Chr2:12152573-12152592 None:intergenic 40.0%
AGAGCGACACCAAAATTGTT+AGG - Chr2:12154288-12154307 None:intergenic 40.0%
AGGTTGAACTCCAAACTGAA+AGG - Chr2:12152743-12152762 None:intergenic 40.0%
AGTAAAAGGAGCAAGGATTG+AGG + Chr2:12154661-12154680 MsG0280007223.01.T01:three_prime_UTR 40.0%
ATCTGGAAACACGAATTACC+GGG - Chr2:12153470-12153489 None:intergenic 40.0%
ATGAAGAGGACTATAGCTGA+AGG - Chr2:12154366-12154385 None:intergenic 40.0%
ATTGAAGCAACTTGCAAGAG+AGG + Chr2:12153996-12154015 MsG0280007223.01.T01:intron 40.0%
ATTGAGATCGACATGCTCAT+TGG + Chr2:12154022-12154041 MsG0280007223.01.T01:intron 40.0%
ATTGAGGGAAGTTCATTGAG+GGG + Chr2:12154677-12154696 MsG0280007223.01.T01:three_prime_UTR 40.0%
ATTTCATGATTGAGCAGGCA+AGG + Chr2:12152541-12152560 MsG0280007223.01.T01:intron 40.0%
CCAAGATGCTCTACAGTTAA+TGG - Chr2:12154437-12154456 None:intergenic 40.0%
GAATGAAATGAACCAGCTAG+TGG + Chr2:12152444-12152463 MsG0280007223.01.T01:intron 40.0%
GAGCGACACCAAAATTGTTA+GGG - Chr2:12154287-12154306 None:intergenic 40.0%
GATTGAGGGAAGTTCATTGA+GGG + Chr2:12154676-12154695 MsG0280007223.01.T01:three_prime_UTR 40.0%
GCAAGAATGTAGTTAGGTGA+AGG - Chr2:12152763-12152782 None:intergenic 40.0%
GCTTGAATACTGACATGCAA+TGG + Chr2:12152257-12152276 MsG0280007223.01.T01:intron 40.0%
GGTTGAACTCCAAACTGAAA+GGG - Chr2:12152742-12152761 None:intergenic 40.0%
GTAAAAGGAGCAAGGATTGA+GGG + Chr2:12154662-12154681 MsG0280007223.01.T01:three_prime_UTR 40.0%
GTGTTTGTAGGAAAGTGAGT+TGG + Chr2:12151943-12151962 MsG0280007223.01.T01:intron 40.0%
GTTTACCAACTGATAAGCTC+AGG + Chr2:12152565-12152584 MsG0280007223.01.T01:CDS 40.0%
TAAGGGTTTAAAGGAGTTGG+AGG + Chr2:12151726-12151745 MsG0280007223.01.T01:intron 40.0%
TCGATTGTTCAGCTTCACTT+TGG + Chr2:12151799-12151818 MsG0280007223.01.T01:intron 40.0%
TGAGTAGTCCAACCATATTC+AGG - Chr2:12151234-12151253 None:intergenic 40.0%
TTACTGTATTCTTCACCCGT+CGG + Chr2:12154460-12154479 MsG0280007223.01.T01:three_prime_UTR 40.0%
TTCACTTTGGACTACTAGAG+TGG + Chr2:12151812-12151831 MsG0280007223.01.T01:intron 40.0%
TTGAAGCAACTTGCAAGAGA+GGG + Chr2:12153997-12154016 MsG0280007223.01.T01:intron 40.0%
TTGAGATCGACATGCTCATT+GGG + Chr2:12154023-12154042 MsG0280007223.01.T01:intron 40.0%
! ACAGAGAGTGTAGAAAAGTG+GGG - Chr2:12151148-12151167 None:intergenic 40.0%
! ACTGCAAATTGATTTCGGTC+TGG - Chr2:12152078-12152097 None:intergenic 40.0%
! AGCTCAACTAGTCTGAACTA+AGG + Chr2:12151708-12151727 MsG0280007223.01.T01:intron 40.0%
! ATATACTTGATGGGCAAGAG+CGG - Chr2:12154524-12154543 None:intergenic 40.0%
! CACAGAGAGTGTAGAAAAGT+GGG - Chr2:12151149-12151168 None:intergenic 40.0%
! CCATTAACTGTAGAGCATCT+TGG + Chr2:12154434-12154453 MsG0280007223.01.T01:exon 40.0%
! GCTCAACTAGTCTGAACTAA+GGG + Chr2:12151709-12151728 MsG0280007223.01.T01:intron 40.0%
! GGATGCATCTCTTGATGTTT+TGG + Chr2:12154197-12154216 MsG0280007223.01.T01:CDS 40.0%
! GTTGGTTGCCCTAACAATTT+TGG + Chr2:12154276-12154295 MsG0280007223.01.T01:intron 40.0%
!! TAAACTCGGTTTGGTATTGC+AGG + Chr2:12153742-12153761 MsG0280007223.01.T01:intron 40.0%
!! TAAACTGTGGCTTTGGATTC+AGG + Chr2:12153088-12153107 MsG0280007223.01.T01:intron 40.0%
!! TGAGTTGATATTCTCTTCGG+TGG - Chr2:12154402-12154421 None:intergenic 40.0%
!! TGGTGCTGTCTATTTAATCC+AGG + Chr2:12152608-12152627 MsG0280007223.01.T01:CDS 40.0%
AGTGAGTGGAATCTGTGACA+AGG - Chr2:12151125-12151144 None:intergenic 45.0%
CCATGAAGATGAATCGGAGA+AGG - Chr2:12151097-12151116 None:intergenic 45.0%
CCTTCTCCGATTCATCTTCA+TGG + Chr2:12151094-12151113 MsG0280007223.01.T01:CDS 45.0%
CGTTTCCCAATTGAGTCGAA+GGG + Chr2:12153507-12153526 MsG0280007223.01.T01:CDS 45.0%
CTGGGCTGAAATTTATCACC+AGG + Chr2:12152367-12152386 MsG0280007223.01.T01:intron 45.0%
CTTCATGAGAGTTTGAGCAC+CGG - Chr2:12151753-12151772 None:intergenic 45.0%
GAGAAGAAAGAAGCTGCAAG+AGG + Chr2:12153530-12153549 MsG0280007223.01.T01:CDS 45.0%
GATAATCAGCAGTTGCTGCT+CGG + Chr2:12154571-12154590 MsG0280007223.01.T01:three_prime_UTR 45.0%
GCAATTTGCGTAGGATGAAG+AGG - Chr2:12154380-12154399 None:intergenic 45.0%
GGATTGAGGGAAGTTCATTG+AGG + Chr2:12154675-12154694 MsG0280007223.01.T01:three_prime_UTR 45.0%
GTTGAGAAAAACACAAGCCC+AGG - Chr2:12152326-12152345 None:intergenic 45.0%
GTTTAAAGGAGTTGGAGGAC+CGG + Chr2:12151731-12151750 MsG0280007223.01.T01:intron 45.0%
GTTTCCCAATTGAGTCGAAG+GGG + Chr2:12153508-12153527 MsG0280007223.01.T01:CDS 45.0%
TGAGATCGACATGCTCATTG+GGG + Chr2:12154024-12154043 MsG0280007223.01.T01:intron 45.0%
TTCTCCCCTTCGACTCAATT+GGG - Chr2:12153515-12153534 None:intergenic 45.0%
TTTAATGGCGCTGCAGTTCA+TGG + Chr2:12154490-12154509 MsG0280007223.01.T01:three_prime_UTR 45.0%
! ATTGATTTCGGTCTGGTTGC+TGG - Chr2:12152071-12152090 None:intergenic 45.0%
! GCACAGAGAGTGTAGAAAAG+TGG - Chr2:12151150-12151169 None:intergenic 45.0%
! GTGTCGCTCTCTTAACATAG+TGG + Chr2:12154298-12154317 MsG0280007223.01.T01:intron 45.0%
!! CGTGCATGGTTTGCTTGTTT+AGG + Chr2:12152143-12152162 MsG0280007223.01.T01:intron 45.0%
!! TGGTGCTGTTAGATCTTCCT+GGG + Chr2:12152306-12152325 MsG0280007223.01.T01:intron 45.0%
!! TTGGTGCTGTTAGATCTTCC+TGG + Chr2:12152305-12152324 MsG0280007223.01.T01:intron 45.0%
!!! CTTCGTTTTTAGTGGAGGTG+AGG + Chr2:12151275-12151294 MsG0280007223.01.T01:intron 45.0%
AGAGGACTATAGCTGAAGGC+AGG - Chr2:12154362-12154381 None:intergenic 50.0%
ATCGACATGCTCATTGGGGA+GGG + Chr2:12154028-12154047 MsG0280007223.01.T01:CDS 50.0%
CGGTGCTCAAACTCTCATGA+AGG + Chr2:12151751-12151770 MsG0280007223.01.T01:intron 50.0%
CTTCTCCCCTTCGACTCAAT+TGG - Chr2:12153516-12153535 None:intergenic 50.0%
GCAGTACGTGCTCTTGTGTT+TGG + Chr2:12151395-12151414 MsG0280007223.01.T01:intron 50.0%
GCGTTTCCCAATTGAGTCGA+AGG + Chr2:12153506-12153525 MsG0280007223.01.T01:CDS 50.0%
GTAGAAAAGTGGGGAGTGAG+TGG - Chr2:12151139-12151158 None:intergenic 50.0%
TCTTCGGTGGCAATTTGCGT+AGG - Chr2:12154389-12154408 None:intergenic 50.0%
TGGAAACACGAATTACCGGG+AGG - Chr2:12153467-12153486 None:intergenic 50.0%
!! CTCGGTTTGGTATTGCAGGT+TGG + Chr2:12153746-12153765 MsG0280007223.01.T01:intron 50.0%
!! CTGTGGCTTTGGATTCAGGT+TGG + Chr2:12153092-12153111 MsG0280007223.01.T01:intron 50.0%
CAGCAGTTGCTGCTCGGTTA+GGG + Chr2:12154577-12154596 MsG0280007223.01.T01:three_prime_UTR 55.0%
GATCGACATGCTCATTGGGG+AGG + Chr2:12154027-12154046 MsG0280007223.01.T01:intron 55.0%
TCAGCAGTTGCTGCTCGGTT+AGG + Chr2:12154576-12154595 MsG0280007223.01.T01:three_prime_UTR 55.0%
TCGACATGCTCATTGGGGAG+GGG + Chr2:12154029-12154048 MsG0280007223.01.T01:CDS 55.0%
CGACATGCTCATTGGGGAGG+GGG + Chr2:12154030-12154049 MsG0280007223.01.T01:CDS 60.0%
Chromosome Type Strat End Strand Name
Chr2 gene 12151047 12154851 12151047 ID=MsG0280007223.01;Name=MsG0280007223.01
Chr2 mRNA 12151047 12154851 12151047 ID=MsG0280007223.01.T01;Parent=MsG0280007223.01;Name=MsG0280007223.01.T01;_AED=0.33;_eAED=0.34;_QI=10|0.62|0.77|1|0.75|0.66|9|411|305
Chr2 exon 12151047 12151291 12151047 ID=MsG0280007223.01.T01:exon:3662;Parent=MsG0280007223.01.T01
Chr2 exon 12151399 12151451 12151399 ID=MsG0280007223.01.T01:exon:3663;Parent=MsG0280007223.01.T01
Chr2 exon 12152558 12152629 12152558 ID=MsG0280007223.01.T01:exon:3664;Parent=MsG0280007223.01.T01
Chr2 exon 12153110 12153182 12153110 ID=MsG0280007223.01.T01:exon:3665;Parent=MsG0280007223.01.T01
Chr2 exon 12153308 12153353 12153308 ID=MsG0280007223.01.T01:exon:3666;Parent=MsG0280007223.01.T01
Chr2 exon 12153488 12153555 12153488 ID=MsG0280007223.01.T01:exon:3667;Parent=MsG0280007223.01.T01
Chr2 exon 12153764 12153817 12153764 ID=MsG0280007223.01.T01:exon:3668;Parent=MsG0280007223.01.T01
Chr2 exon 12154028 12154251 12154028 ID=MsG0280007223.01.T01:exon:3669;Parent=MsG0280007223.01.T01
Chr2 exon 12154348 12154851 12154348 ID=MsG0280007223.01.T01:exon:3670;Parent=MsG0280007223.01.T01
Chr2 five_prime_UTR 12151047 12151056 12151047 ID=MsG0280007223.01.T01:five_prime_utr;Parent=MsG0280007223.01.T01
Chr2 CDS 12151057 12151291 12151057 ID=MsG0280007223.01.T01:cds;Parent=MsG0280007223.01.T01
Chr2 CDS 12151399 12151451 12151399 ID=MsG0280007223.01.T01:cds;Parent=MsG0280007223.01.T01
Chr2 CDS 12152558 12152629 12152558 ID=MsG0280007223.01.T01:cds;Parent=MsG0280007223.01.T01
Chr2 CDS 12153110 12153182 12153110 ID=MsG0280007223.01.T01:cds;Parent=MsG0280007223.01.T01
Chr2 CDS 12153308 12153353 12153308 ID=MsG0280007223.01.T01:cds;Parent=MsG0280007223.01.T01
Chr2 CDS 12153488 12153555 12153488 ID=MsG0280007223.01.T01:cds;Parent=MsG0280007223.01.T01
Chr2 CDS 12153764 12153817 12153764 ID=MsG0280007223.01.T01:cds;Parent=MsG0280007223.01.T01
Chr2 CDS 12154028 12154251 12154028 ID=MsG0280007223.01.T01:cds;Parent=MsG0280007223.01.T01
Chr2 CDS 12154348 12154440 12154348 ID=MsG0280007223.01.T01:cds;Parent=MsG0280007223.01.T01
Chr2 three_prime_UTR 12154441 12154851 12154441 ID=MsG0280007223.01.T01:three_prime_utr;Parent=MsG0280007223.01.T01
Gene Sequence

>MsG0280007223.01.T01

ATGGAGCAGCTTAAGCTAACACTTTCGCTTTTCTTCTCCTTCTCCGATTCATCTTCATGGTTTCACCTTGTCACAGATTCCACTCACTCCCCACTTTTCTACACTCTCTGTGCTGCTTACTCTCTCATCTCCTTCATTGCTCTTATTCAACTCATCAGAATTGAACTTAGAGTACCTGAATATGGTTGGACTACTCAGAAAATATTTCATCTCTTCAACTTCGTTTTTAGTGGAGTACGTGCTCTTGTGTTTGGATTTCATGCTCAAGTGTTTTTCTTGCATCCTAAGGCAAGGAGTTTACCAACTGATAAGCTCAGGATAGTTTACATCTCTGTAAATGGTGCTGTCTATTTAATCCAGGTTGGTATTTGGATTTACCTCTGGTTAGATTACAATGATGTTGTGGACTTCATTGGAAAGATATTTATAGGAGGTGTATCATTTGTAGCTGCAATAGGATTTCTAATATTTGGAGGAAGATTATTTTTCATGCTGAAGCGTTTCCCAATTGAGTCGAAGGGGAGAAGAAAGAAGCTGCAAGAGGTATGTTGGATTTGTGACAATCATATGTTTCGCCTGTTTTCTGATAAGATCTATGATGATCGACATGCTCATTGGGGAGGGGGACTTAGTACTTGTCAATTGTCCGGAGAAATTGCAAACAAAATCTCAAGGATTAAATTTAATATATGTGAGGTGTTATTATTCAAGTGCTTCTTTCGTAATTACATTTTGTTACACATGCAGGTTCTTGTATCTGCAGTTGATTCGGATGCATCTCTTGATGTTTTGGACCATCCTATTTTGGACTTCATCTACTACATGGTGGTTGAGATCCTGCCTTCAGCTATAGTCCTCTTCATCCTACGCAAATTGCCACCGAAGAGAATATCAACTCAATATCATGACATCCATTAA
Protein sequence

>MsG0280007223.01.T01

MEQLKLTLSLFFSFSDSSSWFHLVTDSTHSPLFYTLCAAYSLISFIALIQLIRIELRVPEYGWTTQKIFHLFNFVFSGVRALVFGFHAQVFFLHPKARSLPTDKLRIVYISVNGAVYLIQVGIWIYLWLDYNDVVDFIGKIFIGGVSFVAAIGFLIFGGRLFFMLKRFPIESKGRRKKLQEVCWICDNHMFRLFSDKIYDDRHAHWGGGLSTCQLSGEIANKISRIKFNICEVLLFKCFFRNYILLHMQVLVSAVDSDASLDVLDHPILDFIYYMVVEILPSAIVLFILRKLPPKRISTQYHDIH*