Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MsG0280007479.01.T01 | XP_003594241.1 | 92.5 | 120 | 6 | 2 | 1 | 120 | 1 | 117 | 8.84E-68 | 209 |
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MsG0280007479.01.T01 | Q41916 | 64.835 | 91 | 31 | 1 | 30 | 119 | 26 | 116 | 9.51E-36 | 121 |
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MsG0280007479.01.T01 | G7IM41 | 92.500 | 120 | 6 | 2 | 1 | 120 | 1 | 117 | 4.22e-68 | 209 |
| Gene ID | Type | Classification |
|---|
| Gene ID | Type | Classification |
|---|
Co-expression Network
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|---|---|---|---|
| MsG0080049052.01 | MsG0280007479.01 | 0.807421 | 5.095558e-50 | 2.836955e-47 |
| MsG0180003088.01 | MsG0280007479.01 | 0.810093 | 1.368214e-50 | 8.172876e-48 |
| MsG0280007479.01 | MsG0580024575.01 | 0.810739 | 9.923822e-51 | 6.030325e-48 |
| MsG0280007479.01 | MsG0680035582.01 | 0.814723 | 1.332651e-51 | 9.011044e-49 |
| MsG0280007479.01 | MsG0780039841.01 | 0.801622 | 8.256240e-49 | 3.961024e-46 |
PPI
| Gene1 | Gene2 | Type |
|---|
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MsG0280007479.01.T01 | MTR_2g026040 | 92.500 | 120 | 6 | 2 | 1 | 120 | 1 | 117 | 1.07e-71 | 209 |
| MsG0280007479.01.T01 | MTR_4g133540 | 66.667 | 87 | 29 | 0 | 33 | 119 | 29 | 115 | 2.04e-39 | 128 |
| MsG0280007479.01.T01 | MTR_2g026040 | 82.353 | 85 | 11 | 3 | 1 | 85 | 1 | 81 | 7.42e-39 | 125 |
| MsG0280007479.01.T01 | MTR_4g017620 | 55.046 | 109 | 46 | 2 | 14 | 119 | 6 | 114 | 4.36e-33 | 112 |
| MsG0280007479.01.T01 | MTR_4g095630 | 44.538 | 119 | 59 | 1 | 1 | 119 | 1 | 112 | 5.55e-29 | 101 |
| MsG0280007479.01.T01 | MTR_4g040310 | 53.846 | 91 | 40 | 1 | 30 | 120 | 22 | 110 | 3.36e-28 | 99.8 |
| MsG0280007479.01.T01 | MTR_2g103740 | 52.174 | 92 | 42 | 1 | 27 | 118 | 20 | 109 | 3.46e-28 | 99.8 |
| MsG0280007479.01.T01 | MTR_2g076800 | 44.167 | 120 | 58 | 2 | 1 | 119 | 1 | 112 | 1.67e-27 | 98.2 |
| MsG0280007479.01.T01 | MTR_5g091280 | 42.857 | 119 | 58 | 5 | 1 | 117 | 1 | 111 | 1.69e-24 | 90.5 |
| MsG0280007479.01.T01 | MTR_2g076740 | 42.241 | 116 | 59 | 1 | 1 | 116 | 1 | 108 | 9.97e-23 | 85.9 |
| MsG0280007479.01.T01 | MTR_8g079410 | 48.889 | 90 | 42 | 2 | 32 | 117 | 25 | 114 | 7.93e-22 | 83.6 |
| MsG0280007479.01.T01 | MTR_0219s0010 | 43.617 | 94 | 52 | 1 | 27 | 119 | 19 | 112 | 3.37e-20 | 79.3 |
| MsG0280007479.01.T01 | MTR_5g098550 | 40.187 | 107 | 60 | 3 | 12 | 116 | 5 | 109 | 3.39e-20 | 79.3 |
| MsG0280007479.01.T01 | MTR_5g091260 | 48.864 | 88 | 42 | 3 | 34 | 119 | 27 | 113 | 7.63e-20 | 78.6 |
| MsG0280007479.01.T01 | MTR_5g091330 | 38.938 | 113 | 63 | 4 | 9 | 119 | 5 | 113 | 2.19e-16 | 69.7 |
| MsG0280007479.01.T01 | MTR_8g041410 | 39.326 | 89 | 47 | 1 | 36 | 117 | 40 | 128 | 1.10e-15 | 70.5 |
| MsG0280007479.01.T01 | MTR_6g078440 | 44.304 | 79 | 42 | 2 | 40 | 116 | 79 | 157 | 2.76e-15 | 68.2 |
| MsG0280007479.01.T01 | MTR_3g043750 | 38.462 | 91 | 55 | 1 | 30 | 119 | 2 | 92 | 5.72e-15 | 65.5 |
| MsG0280007479.01.T01 | MTR_5g098560 | 39.796 | 98 | 42 | 2 | 22 | 119 | 15 | 95 | 6.95e-15 | 65.5 |
| MsG0280007479.01.T01 | MTR_5g088770 | 36.957 | 92 | 57 | 1 | 30 | 120 | 2 | 93 | 1.73e-14 | 64.7 |
| MsG0280007479.01.T01 | MTR_0437s0020 | 44.304 | 79 | 41 | 3 | 40 | 116 | 26 | 103 | 2.85e-14 | 64.3 |
| MsG0280007479.01.T01 | MTR_3g451210 | 34.862 | 109 | 69 | 2 | 12 | 119 | 12 | 119 | 4.70e-14 | 64.3 |
| MsG0280007479.01.T01 | MTR_3g452280 | 34.862 | 109 | 69 | 2 | 12 | 119 | 12 | 119 | 4.70e-14 | 64.3 |
| MsG0280007479.01.T01 | MTR_2g028080 | 50.000 | 68 | 32 | 2 | 40 | 105 | 26 | 93 | 3.91e-13 | 61.2 |
| MsG0280007479.01.T01 | MTR_5g091360 | 43.038 | 79 | 42 | 3 | 40 | 116 | 32 | 109 | 5.56e-13 | 60.8 |
| MsG0280007479.01.T01 | MTR_2g028520 | 43.662 | 71 | 36 | 2 | 40 | 106 | 26 | 96 | 3.48e-11 | 56.2 |
| MsG0280007479.01.T01 | MTR_0013s0070 | 44.578 | 83 | 43 | 3 | 40 | 120 | 26 | 107 | 6.90e-11 | 55.5 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MsG0280007479.01.T01 | AT5G47550 | 64.835 | 91 | 31 | 1 | 30 | 119 | 26 | 116 | 9.70e-37 | 121 |
| MsG0280007479.01.T01 | AT4G16500 | 62.195 | 82 | 30 | 1 | 37 | 117 | 34 | 115 | 2.33e-26 | 95.1 |
| MsG0280007479.01.T01 | AT4G16500 | 62.195 | 82 | 30 | 1 | 37 | 117 | 34 | 115 | 2.33e-26 | 95.1 |
| MsG0280007479.01.T01 | AT2G40880 | 40.860 | 93 | 54 | 1 | 27 | 118 | 30 | 122 | 2.53e-20 | 80.1 |
| MsG0280007479.01.T01 | AT3G12490 | 37.931 | 116 | 69 | 2 | 8 | 120 | 11 | 126 | 1.76e-17 | 75.5 |
| MsG0280007479.01.T01 | AT3G12490 | 42.391 | 92 | 52 | 1 | 30 | 120 | 2 | 93 | 3.54e-17 | 72.8 |
| MsG0280007479.01.T01 | AT5G12140 | 40.000 | 90 | 53 | 1 | 31 | 119 | 9 | 98 | 1.56e-15 | 67.0 |
| MsG0280007479.01.T01 | AT5G05110 | 37.778 | 90 | 54 | 1 | 32 | 119 | 45 | 134 | 5.57e-14 | 65.9 |
Find 31 sgRNAs with CRISPR-Local
Find 28 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| GGTGAGACACAAGTGGTTTC+TGG | 0.279706 | 2:+16014447 | MsG0280007479.01.T01:CDS |
| GGGTCTTTCACGTTCTTGAT+TGG | 0.308449 | 2:-16014339 | None:intergenic |
| GGTCTTTCACGTTCTTGATT+GGG | 0.329181 | 2:-16014338 | None:intergenic |
| AGAATTATGAAGCACGTGTT+TGG | 0.333246 | 2:+16014517 | MsG0280007479.01.T01:CDS |
| GAATTATGAAGCACGTGTTT+GGG | 0.379153 | 2:+16014518 | MsG0280007479.01.T01:CDS |
| AGCATCCTTCTTCAAACCAT+TGG | 0.422878 | 2:-16014298 | None:intergenic |
| GCACCGTTGTGAAGTTTCAT+TGG | 0.424065 | 2:-16014228 | None:intergenic |
| CGTTTCCAATGGTTTGAAGA+AGG | 0.442519 | 2:+16014293 | MsG0280007479.01.T01:CDS |
| CACGTGTTTGGGAGAAACCT+TGG | 0.458351 | 2:+16014529 | MsG0280007479.01.T01:CDS |
| ACATCACATAATCAAGCAAC+AGG | 0.465380 | 2:-16014582 | None:intergenic |
| ACAGAGTACGGGAAACAATC+TGG | 0.483880 | 2:+16014393 | MsG0280007479.01.T01:CDS |
| CTTCTTCAAACCATTGGAAA+CGG | 0.493775 | 2:-16014292 | None:intergenic |
| AAATTCGCAGTGACAGAGTA+CGG | 0.494043 | 2:+16014381 | MsG0280007479.01.T01:CDS |
| TCGGTCTTTACCGTTTCCAA+TGG | 0.496229 | 2:+16014282 | MsG0280007479.01.T01:CDS |
| TCTCTTTCTATCTGTTGTGT+CGG | 0.500012 | 2:+16014263 | MsG0280007479.01.T01:CDS |
| GTCTTTCACGTTCTTGATTG+GGG | 0.523162 | 2:-16014337 | None:intergenic |
| TTGAAGAAGGATGCTCTTGT+CGG | 0.542380 | 2:+16014306 | MsG0280007479.01.T01:CDS |
| TTCGCGATCTCAACCACCTG+TGG | 0.547556 | 2:-16014360 | None:intergenic |
| AAGAAGGATGCTCTTGTCGG+TGG | 0.552279 | 2:+16014309 | MsG0280007479.01.T01:CDS |
| AGATTCTTCAAATGAAGCCA+AGG | 0.557868 | 2:-16014546 | None:intergenic |
| AATTCGCAGTGACAGAGTAC+GGG | 0.560575 | 2:+16014382 | MsG0280007479.01.T01:CDS |
| TACGAATTACCGTCTTGTGT+TGG | 0.594867 | 2:+16014470 | MsG0280007479.01.T01:CDS |
| AGGATGCTCTTGTCGGTGGG+TGG | 0.595498 | 2:+16014313 | MsG0280007479.01.T01:CDS |
| TCGCGATCTCAACCACCTGT+GGG | 0.600413 | 2:-16014359 | None:intergenic |
| CTTTCGCAGCCAACACAAGA+CGG | 0.634024 | 2:-16014479 | None:intergenic |
| CAAGAACGTGAAAGACCCAC+AGG | 0.645956 | 2:+16014344 | MsG0280007479.01.T01:CDS |
| AGTTTCGTGAAAGTGATCAA+AGG | 0.652758 | 2:+16014426 | MsG0280007479.01.T01:CDS |
| AGAAGGATGCTCTTGTCGGT+GGG | 0.667040 | 2:+16014310 | MsG0280007479.01.T01:CDS |
| GATCAAAGGTGAGACACAAG+TGG | 0.672303 | 2:+16014440 | MsG0280007479.01.T01:CDS |
| GAACGTGAAAGACCCACAGG+TGG | 0.721214 | 2:+16014347 | MsG0280007479.01.T01:CDS |
| TTACCAATGAAACTTCACAA+CGG | 0.778595 | 2:+16014225 | None:intergenic |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|---|---|---|---|---|
| AGAATTATGAAGCACGTGTT+TGG | + | Chr2:16014517-16014536 | MsG0280007479.01.T01:CDS | 35.0% | |
| AGATTCTTCAAATGAAGCCA+AGG | - | Chr2:16014549-16014568 | None:intergenic | 35.0% | |
| AGTTTCGTGAAAGTGATCAA+AGG | + | Chr2:16014426-16014445 | MsG0280007479.01.T01:CDS | 35.0% | |
| CTTCTTCAAACCATTGGAAA+CGG | - | Chr2:16014295-16014314 | None:intergenic | 35.0% | |
| GAATTATGAAGCACGTGTTT+GGG | + | Chr2:16014518-16014537 | MsG0280007479.01.T01:CDS | 35.0% | |
| TCTCTTTCTATCTGTTGTGT+CGG | + | Chr2:16014263-16014282 | MsG0280007479.01.T01:CDS | 35.0% | |
| AAATTCGCAGTGACAGAGTA+CGG | + | Chr2:16014381-16014400 | MsG0280007479.01.T01:CDS | 40.0% | |
| AGCATCCTTCTTCAAACCAT+TGG | - | Chr2:16014301-16014320 | None:intergenic | 40.0% | |
| CGTTTCCAATGGTTTGAAGA+AGG | + | Chr2:16014293-16014312 | MsG0280007479.01.T01:CDS | 40.0% | |
| GGTCTTTCACGTTCTTGATT+GGG | - | Chr2:16014341-16014360 | None:intergenic | 40.0% | |
| GTCTTTCACGTTCTTGATTG+GGG | - | Chr2:16014340-16014359 | None:intergenic | 40.0% | |
| TACGAATTACCGTCTTGTGT+TGG | + | Chr2:16014470-16014489 | MsG0280007479.01.T01:CDS | 40.0% | |
| TTGAAGAAGGATGCTCTTGT+CGG | + | Chr2:16014306-16014325 | MsG0280007479.01.T01:CDS | 40.0% | |
| AATTCGCAGTGACAGAGTAC+GGG | + | Chr2:16014382-16014401 | MsG0280007479.01.T01:CDS | 45.0% | |
| ACAGAGTACGGGAAACAATC+TGG | + | Chr2:16014393-16014412 | MsG0280007479.01.T01:CDS | 45.0% | |
| GATCAAAGGTGAGACACAAG+TGG | + | Chr2:16014440-16014459 | MsG0280007479.01.T01:CDS | 45.0% | |
| GGGTCTTTCACGTTCTTGAT+TGG | - | Chr2:16014342-16014361 | None:intergenic | 45.0% | |
| TCGGTCTTTACCGTTTCCAA+TGG | + | Chr2:16014282-16014301 | MsG0280007479.01.T01:CDS | 45.0% | |
| AAGAAGGATGCTCTTGTCGG+TGG | + | Chr2:16014309-16014328 | MsG0280007479.01.T01:CDS | 50.0% | |
| AGAAGGATGCTCTTGTCGGT+GGG | + | Chr2:16014310-16014329 | MsG0280007479.01.T01:CDS | 50.0% | |
| CAAGAACGTGAAAGACCCAC+AGG | + | Chr2:16014344-16014363 | MsG0280007479.01.T01:CDS | 50.0% | |
| CACGTGTTTGGGAGAAACCT+TGG | + | Chr2:16014529-16014548 | MsG0280007479.01.T01:CDS | 50.0% | |
| CTTTCGCAGCCAACACAAGA+CGG | - | Chr2:16014482-16014501 | None:intergenic | 50.0% | |
| GGTGAGACACAAGTGGTTTC+TGG | + | Chr2:16014447-16014466 | MsG0280007479.01.T01:CDS | 50.0% | |
| GAACGTGAAAGACCCACAGG+TGG | + | Chr2:16014347-16014366 | MsG0280007479.01.T01:CDS | 55.0% | |
| TCGCGATCTCAACCACCTGT+GGG | - | Chr2:16014362-16014381 | None:intergenic | 55.0% | |
| TTCGCGATCTCAACCACCTG+TGG | - | Chr2:16014363-16014382 | None:intergenic | 55.0% | |
| AGGATGCTCTTGTCGGTGGG+TGG | + | Chr2:16014313-16014332 | MsG0280007479.01.T01:CDS | 60.0% |
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| Chr2 | gene | 16014231 | 16014593 | 16014231 | ID=MsG0280007479.01;Name=MsG0280007479.01 |
| Chr2 | mRNA | 16014231 | 16014593 | 16014231 | ID=MsG0280007479.01.T01;Parent=MsG0280007479.01;Name=MsG0280007479.01.T01;_AED=0.50;_eAED=0.50;_QI=0|-1|0|1|-1|1|1|0|120 |
| Chr2 | exon | 16014231 | 16014593 | 16014231 | ID=MsG0280007479.01.T01:exon:7380;Parent=MsG0280007479.01.T01 |
| Chr2 | CDS | 16014231 | 16014593 | 16014231 | ID=MsG0280007479.01.T01:cds;Parent=MsG0280007479.01.T01 |
| Gene Sequence |
| Protein sequence |
