AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar ZhongmuNo.1 / MsG0280007633.01

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MsG0280007633.01.T01 MTR_1g031930 51.466 307 110 6 3 291 50 335 1.42e-89 271
MsG0280007633.01.T01 MTR_1g031930 47.670 279 115 8 31 291 9 274 2.87e-66 209
MsG0280007633.01.T01 MTR_2g029260 85.470 117 17 0 129 245 2 118 4.84e-55 174
MsG0280007633.01.T01 MTR_6g069870 31.285 358 171 13 1 294 16 362 3.29e-34 128
MsG0280007633.01.T01 MTR_6g069870 30.882 340 160 13 1 276 16 344 1.16e-28 112
MsG0280007633.01.T01 MTR_6g069870 32.364 275 161 11 1 265 16 275 3.81e-23 97.4
MsG0280007633.01.T01 MTR_2g042900 36.478 159 68 5 137 290 110 240 1.54e-16 77.8
MsG0280007633.01.T01 MTR_2g042900 35.484 155 77 6 137 276 110 256 1.13e-11 63.9
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MsG0280007633.01.T01 AT3G17860 33.550 307 147 14 32 295 14 306 3.38e-26 105
MsG0280007633.01.T01 AT3G17860 33.550 307 147 14 32 295 14 306 3.38e-26 105
MsG0280007633.01.T01 AT3G17860 33.550 307 147 14 32 295 47 339 3.86e-26 106
MsG0280007633.01.T01 AT3G17860 33.550 307 147 14 32 295 34 326 4.64e-26 105
MsG0280007633.01.T01 AT1G70700 38.136 236 99 8 54 283 50 244 1.06e-23 97.8
MsG0280007633.01.T01 AT1G70700 35.065 231 91 8 54 284 50 221 1.71e-22 94.0
MsG0280007633.01.T01 AT1G70700 36.283 226 103 8 54 276 50 237 3.62e-20 88.6
MsG0280007633.01.T01 AT1G48500 33.466 251 110 12 60 292 82 293 2.29e-17 81.3
MsG0280007633.01.T01 AT1G48500 32.479 234 102 11 60 276 52 246 2.21e-12 66.2
MsG0280007633.01.T01 AT1G48500 32.479 234 102 11 60 276 82 276 2.57e-12 66.2
MsG0280007633.01.T01 AT5G20900 29.560 159 70 4 127 283 45 163 2.94e-11 62.0

Find 82 sgRNAs with CRISPR-Local

Find 162 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
GATTTCTTGTTAATGTTATA+TGG 0.220633 2:+18353037 None:intergenic
TGAAACTGTATTAGCTTGAT+TGG 0.288636 2:+18354411 None:intergenic
TAGTAAAATTACTACGATTC+AGG 0.303233 2:+18354244 None:intergenic
ACATCATGTTGTACAGGATA+TGG 0.307686 2:+18354451 None:intergenic
GAGGAGGCTGGTGCATTTGC+AGG 0.320510 2:+18353651 None:intergenic
CATAAAACCAGCAGATGTTA+TGG 0.358564 2:+18355046 None:intergenic
CATATGAAGCTGCCACTACT+TGG 0.361235 2:-18354328 MsG0280007633.01.T01:CDS
AGTATGCGATGAAATAGATA+TGG 0.369654 2:+18353614 None:intergenic
GGAGGCTCGGCATTGCTAAC+AGG 0.384286 2:+18353516 None:intergenic
TTTAACTGCGAGAAATTCAT+TGG 0.399647 2:+18353560 None:intergenic
CATAGCACATACAGAACTTC+AGG 0.400250 2:-18353703 MsG0280007633.01.T01:CDS
GTTGGTTGAGGCGATGTAAC+AGG 0.403409 2:+18354298 None:intergenic
TTACTACAGTCAAATGCATC+TGG 0.414565 2:+18355020 None:intergenic
TTTCTCGCAGTTAAAACAAC+TGG 0.425409 2:-18353552 MsG0280007633.01.T01:CDS
AAGATGGTAAGTTGGGAAGA+AGG 0.425596 2:+18354113 None:intergenic
CGCATAGAAGATGGTAAGTT+GGG 0.437429 2:+18354106 None:intergenic
TAGAAATCTTTCAATCATGA+TGG 0.440350 2:-18354502 MsG0280007633.01.T01:CDS
GGCACAAGCTATCCTGTTGT+TGG 0.441454 2:-18353754 MsG0280007633.01.T01:CDS
GCCATTACCATGCTGGCGTC+AGG 0.443156 2:-18353480 MsG0280007633.01.T01:intron
CACCATTCCTAGCCAACAAC+AGG 0.452637 2:+18353742 None:intergenic
ATATCTATTTCATCGCATAC+TGG 0.462263 2:-18353612 MsG0280007633.01.T01:CDS
GCAAATGCACCAGCCTCCTC+TGG 0.467713 2:-18353648 MsG0280007633.01.T01:CDS
CCCACCATTGGTGCCGTCGT+TGG 0.469756 2:-18354271 MsG0280007633.01.T01:CDS
ATATGAAGCTGCCACTACTT+GGG 0.472084 2:-18354327 MsG0280007633.01.T01:CDS
TCGCATACTGGTGCACAGTC+AGG 0.473936 2:-18353600 MsG0280007633.01.T01:CDS
ATAAAACCAGCAGATGTTAT+GGG 0.478111 2:+18355047 None:intergenic
TCTGATCCCATAACATCTGC+TGG 0.481214 2:-18355053 MsG0280007633.01.T01:CDS
TGCATTTGCAGGTTGACTCA+CGG 0.482777 2:+18353662 None:intergenic
ACCTGACGCCAGCATGGTAA+TGG 0.485087 2:+18353479 None:intergenic
GTAATGGCATCGATTACTCT+TGG 0.489598 2:+18353495 None:intergenic
AAAACTCTCAGCTGAACCTC+AGG 0.495179 2:-18354997 MsG0280007633.01.T01:intron
AGTTGGGAAGAAGGCAGAGA+TGG 0.495544 2:+18354122 None:intergenic
TCAGGCTCGGAAAGCATCCT+TGG 0.496291 2:-18353301 MsG0280007633.01.T01:CDS
AGAAATCTTTCAATCATGAT+GGG 0.503355 2:-18354501 MsG0280007633.01.T01:CDS
AAGCTATCCTGTTGTTGGCT+AGG 0.504599 2:-18353749 MsG0280007633.01.T01:CDS
ATCGATTACTCTTGGAGGCT+CGG 0.505842 2:+18353503 None:intergenic
CAACTTACCATCTTCTATGC+GGG 0.506683 2:-18354104 MsG0280007633.01.T01:CDS
TCCAACGACGGCACCAATGG+TGG 0.507504 2:+18354270 None:intergenic
ATCTTCACATCATGTTGTAC+AGG 0.507813 2:+18354445 None:intergenic
AAGAGAGATACCTTGTCGGC+AGG 0.512991 2:+18354056 None:intergenic
GGCATTGCTAACAGGAGTGA+TGG 0.514325 2:+18353524 None:intergenic
CAACCAACTCTTCCCACCAT+TGG 0.522868 2:-18354283 MsG0280007633.01.T01:CDS
CCGCATAGAAGATGGTAAGT+TGG 0.523235 2:+18354105 None:intergenic
GCATTTGCAGGTTGACTCAC+GGG 0.524091 2:+18353663 None:intergenic
GCAGTTACAAAATGATTCCT+CGG 0.526505 2:+18354373 None:intergenic
ATCCTGTTGTTGGCTAGGAA+TGG 0.530502 2:-18353744 MsG0280007633.01.T01:CDS
AACAAAGAGAGATACCTTGT+CGG 0.533016 2:+18354052 None:intergenic
TATGAAGCTGCCACTACTTG+GGG 0.537617 2:-18354326 MsG0280007633.01.T01:CDS
CTCACGGGCACACCGTCACC+AGG 0.540218 2:+18353678 None:intergenic
CAGTTACAAAATGATTCCTC+GGG 0.542977 2:+18354374 None:intergenic
GCACCAATGGTGGGAAGAGT+TGG 0.549558 2:+18354280 None:intergenic
GACGGTACCCGCATAGAAGA+TGG 0.552065 2:+18354097 None:intergenic
CTTGGATGACCAGAGGAGGC+TGG 0.553563 2:+18353639 None:intergenic
CAATGACGTCCCTGCCGACA+AGG 0.559227 2:-18354066 MsG0280007633.01.T01:intron
ATGCAGCGATACCTCAGGCT+CGG 0.560553 2:-18353314 MsG0280007633.01.T01:intron
GACGTCATTGAAAACATTGA+CGG 0.563003 2:+18354079 None:intergenic
AGAGAGATACCTTGTCGGCA+GGG 0.565241 2:+18354057 None:intergenic
ATGGCATCGATTACTCTTGG+AGG 0.566283 2:+18353498 None:intergenic
CATTCCAACGACGGCACCAA+TGG 0.572081 2:+18354267 None:intergenic
AATCGATGCCATTACCATGC+TGG 0.572186 2:-18353487 MsG0280007633.01.T01:CDS
ACTGGTGCACAGTCAGGCAG+TGG 0.578273 2:-18353594 MsG0280007633.01.T01:CDS
GTATGCGATGAAATAGATAT+GGG 0.584760 2:+18353615 None:intergenic
CATACAGAACTTCAGGCACC+TGG 0.590450 2:-18353696 MsG0280007633.01.T01:CDS
GAACTTCAGGCACCTGGTGA+CGG 0.594058 2:-18353690 MsG0280007633.01.T01:CDS
ACTGCTGGTCAGAATATGAA+TGG 0.602374 2:-18354355 MsG0280007633.01.T01:CDS
GCATTGCTAACAGGAGTGAT+GGG 0.603727 2:+18353525 None:intergenic
TTCTAGAAAAGCGCAAGGAG+AGG 0.607349 2:-18353272 MsG0280007633.01.T01:intron
GGCTAGGAATGGTGTATCAG+TGG 0.612360 2:-18353733 MsG0280007633.01.T01:CDS
GATGAAATAGATATGGGACT+TGG 0.616222 2:+18353621 None:intergenic
TCTTTCAATCATGATGGGCA+AGG 0.623986 2:-18354496 MsG0280007633.01.T01:CDS
CCAACGACGGCACCAATGGT+GGG 0.628134 2:+18354271 None:intergenic
CAGCATACCTGACGCCAGCA+TGG 0.631084 2:+18353473 None:intergenic
CATTGACGAACTCATTCCCG+AGG 0.632457 2:-18354390 MsG0280007633.01.T01:CDS
ATGAAGCTGCCACTACTTGG+GGG 0.637753 2:-18354325 MsG0280007633.01.T01:CDS
AAGGATGCTTTCCGAGCCTG+AGG 0.641286 2:+18353303 None:intergenic
TTCAATCATGATGGGCAAGG+TGG 0.642760 2:-18354493 MsG0280007633.01.T01:CDS
CGATTCAGGCATTCCAACGA+CGG 0.643026 2:+18354258 None:intergenic
CCAACTTACCATCTTCTATG+CGG 0.674088 2:-18354105 MsG0280007633.01.T01:CDS
TGAAGCTGCCACTACTTGGG+GGG 0.678942 2:-18354324 MsG0280007633.01.T01:CDS
GGGACTTGGATGACCAGAGG+AGG 0.679930 2:+18353635 None:intergenic
TATGGGACTTGGATGACCAG+AGG 0.694039 2:+18353632 None:intergenic
ATGGTGGGAAGAGTTGGTTG+AGG 0.716568 2:+18354286 None:intergenic

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!!! ATTTTACTTTTATAAAGAAA+AGG + Chr2:18354876-18354895 None:intergenic 10.0%
!! AAATTATTAAATGTGTAGAA+TGG + Chr2:18354761-18354780 None:intergenic 15.0%
!! ACTTAGTAGTATATTAATTA+AGG - Chr2:18353448-18353467 MsG0280007633.01.T01:intron 15.0%
!! CTTAGTAGTATATTAATTAA+GGG - Chr2:18353449-18353468 MsG0280007633.01.T01:intron 15.0%
!! TAAATAAATACAAGGTAAAA+AGG - Chr2:18354098-18354117 MsG0280007633.01.T01:CDS 15.0%
!! TTTACCTTGTATTTATTTAT+AGG + Chr2:18354097-18354116 None:intergenic 15.0%
!! AGGAAAAAAAAAATGAATTG+TGG + Chr2:18354124-18354143 None:intergenic 20.0%
!! GATTTCTTGTTAATGTTATA+TGG + Chr2:18355076-18355095 None:intergenic 20.0%
!! GTTTATGAACATTTAACATT+TGG - Chr2:18353379-18353398 MsG0280007633.01.T01:intron 20.0%
!! TTGTTTATTGAAATCTTGTT+TGG - Chr2:18354695-18354714 MsG0280007633.01.T01:intron 20.0%
!!! TGTAACATTTTTTACATTGA+CGG - Chr2:18353309-18353328 MsG0280007633.01.T01:CDS 20.0%
!!! TGTTTATTGAAATCTTGTTT+GGG - Chr2:18354696-18354715 MsG0280007633.01.T01:intron 20.0%
! AAAAGTAATGTGTGTAGAAA+TGG + Chr2:18354926-18354945 None:intergenic 25.0%
! AATTGCAAACAAACAATAGA+AGG + Chr2:18353582-18353601 None:intergenic 25.0%
! AGAAATCTTTCAATCATGAT+GGG - Chr2:18353609-18353628 MsG0280007633.01.T01:CDS 25.0%
! CTTGTACTTGTTTAAATTTG+AGG + Chr2:18353478-18353497 None:intergenic 25.0%
! TAGAAATCTTTCAATCATGA+TGG - Chr2:18353608-18353627 MsG0280007633.01.T01:CDS 25.0%
! TAGTAAAATTACTACGATTC+AGG + Chr2:18353869-18353888 None:intergenic 25.0%
! TCTGCCTATAAATAAATACA+AGG - Chr2:18354090-18354109 MsG0280007633.01.T01:CDS 25.0%
! TGTATTTATTTATAGGCAGA+AGG + Chr2:18354090-18354109 None:intergenic 25.0%
!! GTTTATTGAAATCTTGTTTG+GGG - Chr2:18354697-18354716 MsG0280007633.01.T01:intron 25.0%
!! TTTTTCGTTAACCAAGTTAT+TGG + Chr2:18354289-18354308 None:intergenic 25.0%
!!! GTAACATTTTTTACATTGAC+GGG - Chr2:18353310-18353329 MsG0280007633.01.T01:CDS 25.0%
!!! TAACATTTTTTACATTGACG+GGG - Chr2:18353311-18353330 MsG0280007633.01.T01:CDS 25.0%
AAACAATAGAAGGAAGTGAA+GGG + Chr2:18353572-18353591 None:intergenic 30.0%
ACCATCCTAGATTAGATTTA+AGG - Chr2:18354896-18354915 MsG0280007633.01.T01:intron 30.0%
ACTTGGTTAACGAAAAAACT+TGG - Chr2:18354292-18354311 MsG0280007633.01.T01:CDS 30.0%
AGTATGCGATGAAATAGATA+TGG + Chr2:18354499-18354518 None:intergenic 30.0%
ATAAAACCAGCAGATGTTAT+GGG + Chr2:18353066-18353085 None:intergenic 30.0%
ATATCTATTTCATCGCATAC+TGG - Chr2:18354498-18354517 MsG0280007633.01.T01:CDS 30.0%
CATTTAGTATTGTGTTTGCA+GGG - Chr2:18355036-18355055 MsG0280007633.01.T01:CDS 30.0%
CCATCCTAGATTAGATTTAA+GGG - Chr2:18354897-18354916 MsG0280007633.01.T01:intron 30.0%
CTAACTGCAATAATGAGAAA+TGG + Chr2:18354669-18354688 None:intergenic 30.0%
GTATGCGATGAAATAGATAT+GGG + Chr2:18354498-18354517 None:intergenic 30.0%
TTTAACTGCGAGAAATTCAT+TGG + Chr2:18354553-18354572 None:intergenic 30.0%
! TGAAACTGTATTAGCTTGAT+TGG + Chr2:18353702-18353721 None:intergenic 30.0%
AACAAAGAGAGATACCTTGT+CGG + Chr2:18354061-18354080 None:intergenic 35.0%
AATAGTGCCACAATTAAGCT+TGG + Chr2:18354330-18354349 None:intergenic 35.0%
ACATCATGTTGTACAGGATA+TGG + Chr2:18353662-18353681 None:intergenic 35.0%
ACTATAGCCAAGCTTAATTG+TGG - Chr2:18354320-18354339 MsG0280007633.01.T01:CDS 35.0%
ATCTTCACATCATGTTGTAC+AGG + Chr2:18353668-18353687 None:intergenic 35.0%
ATTAAGCTTGGCTATAGTCA+AGG + Chr2:18354318-18354337 None:intergenic 35.0%
CAAACAATAGAAGGAAGTGA+AGG + Chr2:18353573-18353592 None:intergenic 35.0%
CAGTTACAAAATGATTCCTC+GGG + Chr2:18353739-18353758 None:intergenic 35.0%
CATAAAACCAGCAGATGTTA+TGG + Chr2:18353067-18353086 None:intergenic 35.0%
CCCTTAAATCTAATCTAGGA+TGG + Chr2:18354900-18354919 None:intergenic 35.0%
GACGTCATTGAAAACATTGA+CGG + Chr2:18354034-18354053 None:intergenic 35.0%
GATGAAATAGATATGGGACT+TGG + Chr2:18354492-18354511 None:intergenic 35.0%
GCAGTTACAAAATGATTCCT+CGG + Chr2:18353740-18353759 None:intergenic 35.0%
GCATTTAGTATTGTGTTTGC+AGG - Chr2:18355035-18355054 MsG0280007633.01.T01:CDS 35.0%
GTTGCCCTTAAATCTAATCT+AGG + Chr2:18354904-18354923 None:intergenic 35.0%
TCATTACCCCTCAATTTGAA+GGG + Chr2:18354956-18354975 None:intergenic 35.0%
TTACTACAGTCAAATGCATC+TGG + Chr2:18353093-18353112 None:intergenic 35.0%
TTTCTCGCAGTTAAAACAAC+TGG - Chr2:18354558-18354577 MsG0280007633.01.T01:intron 35.0%
! AATTGTGGCACTATTTCTTC+AGG - Chr2:18354335-18354354 MsG0280007633.01.T01:CDS 35.0%
! AGGAATCATTTTGTAACTGC+TGG - Chr2:18353740-18353759 MsG0280007633.01.T01:CDS 35.0%
! TTTTACATTGACGGGGTATA+TGG - Chr2:18353318-18353337 MsG0280007633.01.T01:intron 35.0%
ACTGCTGGTCAGAATATGAA+TGG - Chr2:18353755-18353774 MsG0280007633.01.T01:CDS 40.0%
ATATGAAGCTGCCACTACTT+GGG - Chr2:18353783-18353802 MsG0280007633.01.T01:intron 40.0%
ATCAGAGATTGGATGATCCA+AGG + Chr2:18354158-18354177 None:intergenic 40.0%
ATGGGGAAAAAATGGTTGAG+AGG - Chr2:18354191-18354210 MsG0280007633.01.T01:intron 40.0%
CAACTTACCATCTTCTATGC+GGG - Chr2:18354006-18354025 MsG0280007633.01.T01:intron 40.0%
CATAGCACATACAGAACTTC+AGG - Chr2:18354407-18354426 MsG0280007633.01.T01:CDS 40.0%
CATTACCCCTCAATTTGAAG+GGG + Chr2:18354955-18354974 None:intergenic 40.0%
CCAACTTACCATCTTCTATG+CGG - Chr2:18354005-18354024 MsG0280007633.01.T01:intron 40.0%
CCAATCAGACTACAATACAC+TGG + Chr2:18353410-18353429 None:intergenic 40.0%
CCAGTGTATTGTAGTCTGAT+TGG - Chr2:18353407-18353426 MsG0280007633.01.T01:intron 40.0%
CGCATAGAAGATGGTAAGTT+GGG + Chr2:18354007-18354026 None:intergenic 40.0%
CTCATTACCCCTCAATTTGA+AGG + Chr2:18354957-18354976 None:intergenic 40.0%
CTTCAAATTGAGGGGTAATG+AGG - Chr2:18354955-18354974 MsG0280007633.01.T01:intron 40.0%
GGTATCGCTGCATAAAAGAT+TGG + Chr2:18354789-18354808 None:intergenic 40.0%
GTAATGATCGACGATTAAGC+TGG - Chr2:18353278-18353297 MsG0280007633.01.T01:CDS 40.0%
! AAGATGGTAAGTTGGGAAGA+AGG + Chr2:18354000-18354019 None:intergenic 40.0%
! AGTTTAAAGCCTGAACAGCA+CGG + Chr2:18353155-18353174 None:intergenic 40.0%
!! CTTTTCTAGAAAACGAGCCA+AGG + Chr2:18354829-18354848 None:intergenic 40.0%
!! GTAATGGCATCGATTACTCT+TGG + Chr2:18354618-18354637 None:intergenic 40.0%
!! TCGTTTTCTAGAAAAGCGCA+AGG - Chr2:18354833-18354852 MsG0280007633.01.T01:intron 40.0%
!! TCTTTCAATCATGATGGGCA+AGG - Chr2:18353614-18353633 MsG0280007633.01.T01:CDS 40.0%
AAAACTCTCAGCTGAACCTC+AGG - Chr2:18353113-18353132 MsG0280007633.01.T01:intron 45.0%
AAAGGGTAGCATCAAACCTG+AGG + Chr2:18353132-18353151 None:intergenic 45.0%
AATCGATGCCATTACCATGC+TGG - Chr2:18354623-18354642 MsG0280007633.01.T01:intron 45.0%
ACAAGGCTCGAATCAGAGAT+TGG + Chr2:18354169-18354188 None:intergenic 45.0%
ATCGATTACTCTTGGAGGCT+CGG + Chr2:18354610-18354629 None:intergenic 45.0%
CATATGAAGCTGCCACTACT+TGG - Chr2:18353782-18353801 MsG0280007633.01.T01:intron 45.0%
CCGCATAGAAGATGGTAAGT+TGG + Chr2:18354008-18354027 None:intergenic 45.0%
CCTTGTGGATGGGGAAAAAA+TGG - Chr2:18354183-18354202 MsG0280007633.01.T01:intron 45.0%
GCATTGCTAACAGGAGTGAT+GGG + Chr2:18354588-18354607 None:intergenic 45.0%
GGTGGATCACACCAATAACT+TGG - Chr2:18354275-18354294 MsG0280007633.01.T01:CDS 45.0%
TATGAAGCTGCCACTACTTG+GGG - Chr2:18353784-18353803 MsG0280007633.01.T01:intron 45.0%
TCTGATCCCATAACATCTGC+TGG - Chr2:18353057-18353076 MsG0280007633.01.T01:intron 45.0%
TGGATGATCCAAGGTCAAGT+TGG + Chr2:18354149-18354168 None:intergenic 45.0%
TTTCCTTGCCAACTTGACCT+TGG - Chr2:18354138-18354157 MsG0280007633.01.T01:intron 45.0%
TTTGCGCCCCTTCAAATTGA+GGG - Chr2:18354946-18354965 MsG0280007633.01.T01:intron 45.0%
! AAGCTATCCTGTTGTTGGCT+AGG - Chr2:18354361-18354380 MsG0280007633.01.T01:CDS 45.0%
! AATCTCTGATTCGAGCCTTG+TGG - Chr2:18354168-18354187 MsG0280007633.01.T01:intron 45.0%
! AGACTAGTCGTCAGCAAAGT+CGG - Chr2:18354254-18354273 MsG0280007633.01.T01:intron 45.0%
! ATCCTGTTGTTGGCTAGGAA+TGG - Chr2:18354366-18354385 MsG0280007633.01.T01:CDS 45.0%
! CTTTTATGCAGCGATACCTC+AGG - Chr2:18354791-18354810 MsG0280007633.01.T01:intron 45.0%
! TGCATTTGCAGGTTGACTCA+CGG + Chr2:18354451-18354470 None:intergenic 45.0%
! TTCTAGAAAAGCGCAAGGAG+AGG - Chr2:18354838-18354857 MsG0280007633.01.T01:intron 45.0%
! TTTTGCGCCCCTTCAAATTG+AGG - Chr2:18354945-18354964 MsG0280007633.01.T01:intron 45.0%
!! ATGGCATCGATTACTCTTGG+AGG + Chr2:18354615-18354634 None:intergenic 45.0%
!! CTGACGACTAGTCTCTGTTT+GGG + Chr2:18354248-18354267 None:intergenic 45.0%
!! TTCAATCATGATGGGCAAGG+TGG - Chr2:18353617-18353636 MsG0280007633.01.T01:CDS 45.0%
!!! CCATTTTTTCCCCATCCACA+AGG + Chr2:18354186-18354205 None:intergenic 45.0%
AAAGCCTGAACAGCACGGAA+AGG + Chr2:18353150-18353169 None:intergenic 50.0%
AAGAGAGATACCTTGTCGGC+AGG + Chr2:18354057-18354076 None:intergenic 50.0%
AAGCCTGAACAGCACGGAAA+GGG + Chr2:18353149-18353168 None:intergenic 50.0%
AGAGAGATACCTTGTCGGCA+GGG + Chr2:18354056-18354075 None:intergenic 50.0%
AGTTGGGAAGAAGGCAGAGA+TGG + Chr2:18353991-18354010 None:intergenic 50.0%
ATGAAGCTGCCACTACTTGG+GGG - Chr2:18353785-18353804 MsG0280007633.01.T01:intron 50.0%
CAACCAACTCTTCCCACCAT+TGG - Chr2:18353827-18353846 MsG0280007633.01.T01:intron 50.0%
CACCATTCCTAGCCAACAAC+AGG + Chr2:18354371-18354390 None:intergenic 50.0%
CATACAGAACTTCAGGCACC+TGG - Chr2:18354414-18354433 MsG0280007633.01.T01:CDS 50.0%
CATTGACGAACTCATTCCCG+AGG - Chr2:18353720-18353739 MsG0280007633.01.T01:CDS 50.0%
CGATTCAGGCATTCCAACGA+CGG + Chr2:18353855-18353874 None:intergenic 50.0%
CTAGTCTCTGTTTGGGAGCA+TGG + Chr2:18354241-18354260 None:intergenic 50.0%
GGCACAAGCTATCCTGTTGT+TGG - Chr2:18354356-18354375 MsG0280007633.01.T01:CDS 50.0%
GGCATTGCTAACAGGAGTGA+TGG + Chr2:18354589-18354608 None:intergenic 50.0%
GGCTAGGAATGGTGTATCAG+TGG - Chr2:18354377-18354396 MsG0280007633.01.T01:CDS 50.0%
GTTGGTTGAGGCGATGTAAC+AGG + Chr2:18353815-18353834 None:intergenic 50.0%
TATGGGACTTGGATGACCAG+AGG + Chr2:18354481-18354500 None:intergenic 50.0%
TTGCGCCCCTTCAAATTGAG+GGG - Chr2:18354947-18354966 MsG0280007633.01.T01:intron 50.0%
! ATGGTGGGAAGAGTTGGTTG+AGG + Chr2:18353827-18353846 None:intergenic 50.0%
! CTGATTCGAGCCTTGTGGAT+GGG - Chr2:18354173-18354192 MsG0280007633.01.T01:intron 50.0%
! GCATTTGCAGGTTGACTCAC+GGG + Chr2:18354450-18354469 None:intergenic 50.0%
! TCTGATTCGAGCCTTGTGGA+TGG - Chr2:18354172-18354191 MsG0280007633.01.T01:intron 50.0%
! TGATTCGAGCCTTGTGGATG+GGG - Chr2:18354174-18354193 MsG0280007633.01.T01:intron 50.0%
!! GATCCAAGGTCAAGTTGGCA+AGG + Chr2:18354144-18354163 None:intergenic 50.0%
!! GCTGACGACTAGTCTCTGTT+TGG + Chr2:18354249-18354268 None:intergenic 50.0%
AAGGATGCTTTCCGAGCCTG+AGG + Chr2:18354810-18354829 None:intergenic 55.0%
ACAGGTATCCCCCCAAGTAG+TGG + Chr2:18353797-18353816 None:intergenic 55.0%
ACCTGACGCCAGCATGGTAA+TGG + Chr2:18354634-18354653 None:intergenic 55.0%
ATGCAGCGATACCTCAGGCT+CGG - Chr2:18354796-18354815 MsG0280007633.01.T01:intron 55.0%
CATTCCAACGACGGCACCAA+TGG + Chr2:18353846-18353865 None:intergenic 55.0%
CTAGTCGTCAGCAAAGTCGG+TGG - Chr2:18354257-18354276 MsG0280007633.01.T01:CDS 55.0%
GAACTTCAGGCACCTGGTGA+CGG - Chr2:18354420-18354439 MsG0280007633.01.T01:CDS 55.0%
GACGGTACCCGCATAGAAGA+TGG + Chr2:18354016-18354035 None:intergenic 55.0%
TCGCATACTGGTGCACAGTC+AGG - Chr2:18354510-18354529 MsG0280007633.01.T01:intron 55.0%
TGAAGCTGCCACTACTTGGG+GGG - Chr2:18353786-18353805 MsG0280007633.01.T01:intron 55.0%
! CTACCCTTTCCGTGCTGTTC+AGG - Chr2:18353143-18353162 MsG0280007633.01.T01:intron 55.0%
! GCACCAATGGTGGGAAGAGT+TGG + Chr2:18353833-18353852 None:intergenic 55.0%
!! TCAGGCTCGGAAAGCATCCT+TGG - Chr2:18354809-18354828 MsG0280007633.01.T01:intron 55.0%
ACTGGTGCACAGTCAGGCAG+TGG - Chr2:18354516-18354535 MsG0280007633.01.T01:intron 60.0%
AGGATAGACCCCGCTGAAGG+TGG - Chr2:18354211-18354230 MsG0280007633.01.T01:intron 60.0%
ATGGCTGACCACCTTCAGCG+GGG + Chr2:18354222-18354241 None:intergenic 60.0%
CAATGACGTCCCTGCCGACA+AGG - Chr2:18354044-18354063 MsG0280007633.01.T01:intron 60.0%
CAGCATACCTGACGCCAGCA+TGG + Chr2:18354640-18354659 None:intergenic 60.0%
CATGCTGGCGTCAGGTATGC+TGG - Chr2:18354638-18354657 MsG0280007633.01.T01:intron 60.0%
CATGGCTGACCACCTTCAGC+GGG + Chr2:18354223-18354242 None:intergenic 60.0%
CCAACGACGGCACCAATGGT+GGG + Chr2:18353842-18353861 None:intergenic 60.0%
CTTGGATGACCAGAGGAGGC+TGG + Chr2:18354474-18354493 None:intergenic 60.0%
GAGAGGATAGACCCCGCTGA+AGG - Chr2:18354208-18354227 MsG0280007633.01.T01:intron 60.0%
GAGGAGGCTGGTGCATTTGC+AGG + Chr2:18354462-18354481 None:intergenic 60.0%
GCAAATGCACCAGCCTCCTC+TGG - Chr2:18354462-18354481 MsG0280007633.01.T01:CDS 60.0%
GCATGGCTGACCACCTTCAG+CGG + Chr2:18354224-18354243 None:intergenic 60.0%
GCCATTACCATGCTGGCGTC+AGG - Chr2:18354630-18354649 MsG0280007633.01.T01:intron 60.0%
GGAGGCTCGGCATTGCTAAC+AGG + Chr2:18354597-18354616 None:intergenic 60.0%
GGGACTTGGATGACCAGAGG+AGG + Chr2:18354478-18354497 None:intergenic 60.0%
TCCAACGACGGCACCAATGG+TGG + Chr2:18353843-18353862 None:intergenic 60.0%
!! CCCACCATTGGTGCCGTCGT+TGG - Chr2:18353839-18353858 MsG0280007633.01.T01:intron 65.0%
CTCACGGGCACACCGTCACC+AGG + Chr2:18354435-18354454 None:intergenic 70.0%
Chromosome Type Strat End Strand Name
Chr2 gene 18353015 18355117 18353015 ID=MsG0280007633.01;Name=MsG0280007633.01
Chr2 mRNA 18353015 18355117 18353015 ID=MsG0280007633.01.T01;Parent=MsG0280007633.01;Name=MsG0280007633.01.T01;_AED=0.45;_eAED=0.45;_QI=0|0|0|1|1|1|6|0|295
Chr2 exon 18354998 18355117 18354998 ID=MsG0280007633.01.T01:exon:11084;Parent=MsG0280007633.01.T01
Chr2 exon 18354256 18354521 18354256 ID=MsG0280007633.01.T01:exon:11083;Parent=MsG0280007633.01.T01
Chr2 exon 18354067 18354154 18354067 ID=MsG0280007633.01.T01:exon:11082;Parent=MsG0280007633.01.T01
Chr2 exon 18353481 18353775 18353481 ID=MsG0280007633.01.T01:exon:11081;Parent=MsG0280007633.01.T01
Chr2 exon 18353273 18353330 18353273 ID=MsG0280007633.01.T01:exon:11080;Parent=MsG0280007633.01.T01
Chr2 exon 18353015 18353075 18353015 ID=MsG0280007633.01.T01:exon:11079;Parent=MsG0280007633.01.T01
Chr2 CDS 18354998 18355117 18354998 ID=MsG0280007633.01.T01:cds;Parent=MsG0280007633.01.T01
Chr2 CDS 18354256 18354521 18354256 ID=MsG0280007633.01.T01:cds;Parent=MsG0280007633.01.T01
Chr2 CDS 18354067 18354154 18354067 ID=MsG0280007633.01.T01:cds;Parent=MsG0280007633.01.T01
Chr2 CDS 18353481 18353775 18353481 ID=MsG0280007633.01.T01:cds;Parent=MsG0280007633.01.T01
Chr2 CDS 18353273 18353330 18353273 ID=MsG0280007633.01.T01:cds;Parent=MsG0280007633.01.T01
Chr2 CDS 18353015 18353075 18353015 ID=MsG0280007633.01.T01:cds;Parent=MsG0280007633.01.T01
Gene Sequence

>MsG0280007633.01.T01

ATGTCTTTTGAGGTTTCTCAAGATGATAATACTAAGATCTTATCTGATCCCATAACATCTGCTGGTTTTATGACTCCAGATGCATTTGACTGTAGTAAAAAACTCTCAGCTGAACCTCAGAAATCTTTCAATCATGATGGGCAAGGTGGCTTTCACTTTTCACTTATTCCATATCCTGTACAACATGATGTGAAGATGTTTTCACTTTCCAATCAAGCTAATACAGTTTCATTGACGAACTCATTCCCGAGGAATCATTTTGTAACTGCTGGTCAGAATATGAATGGTAATCATATGAAGCTGCCACTACTTGGGGGGATACCTGTTACATCGCCTCAACCAACTCTTCCCACCATTGGTGCCGTCGTTGGAATGCCTGAATCGTACAATGTGAAACCATCTCTGCCTTCTTCCCAACTTACCATCTTCTATGCGGGTACCGTCAATGTTTTCAATGACGTCCCTGCCGACAAGGCACAAGCTATCCTGTTGTTGGCTAGGAATGGTGTATCAGTGGCTTCTAACATAGCACATACAGAACTTCAGGCACCTGGTGACGGTGTGCCCGTGAGTCAACCTGCAAATGCACCAGCCTCCTCTGGTCATCCAAGTCCCATATCTATTTCATCGCATACTGGTGCACAGTCAGGCAGTGGCTCAACTAGCACCAATGAATTTCTCGCAGTTAAAACAACTGGTGTTCCCATCACTCCTGTTAGCAATGCCGAGCCTCCAAGAGTAATCGATGCCATTACCATGCTGGCGTCAGCGATACCTCAGGCTCGGAAAGCATCCTTGGCTCGTTTTCTAGAAAAGCGCAAGGAGAGGGTGATGAATGCAGCACCATATAACATTAACAAGAAATCTGAAGAGTGTACTTCATCATAA
Protein sequence

>MsG0280007633.01.T01

MSFEVSQDDNTKILSDPITSAGFMTPDAFDCSKKLSAEPQKSFNHDGQGGFHFSLIPYPVQHDVKMFSLSNQANTVSLTNSFPRNHFVTAGQNMNGNHMKLPLLGGIPVTSPQPTLPTIGAVVGMPESYNVKPSLPSSQLTIFYAGTVNVFNDVPADKAQAILLLARNGVSVASNIAHTELQAPGDGVPVSQPANAPASSGHPSPISISSHTGAQSGSGSTSTNEFLAVKTTGVPITPVSNAEPPRVIDAITMLASAIPQARKASLARFLEKRKERVMNAAPYNINKKSEECTSS*