AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar ZhongmuNo.1 / MsG0280007885.01

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MsG0280007885.01.T01 MTR_2g034810 62.434 567 51 8 1 416 354 909 0.0 598
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MsG0280007885.01.T01 AT3G58650 29.778 225 93 6 1 182 322 524 5.57e-11 65.1

Find 81 sgRNAs with CRISPR-Local

Find 137 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
CCCTCAACTTCTGTTCTAAA+AGG 0.206450 2:+22344894 None:intergenic
GAGGTTGATGCTTATGAATT+TGG 0.255705 2:-22343664 MsG0280007885.01.T01:CDS
GGATTGCTGCTCCTTGTTAA+TGG 0.259788 2:+22345058 None:intergenic
TTGATCAAATATTTATTCTT+TGG 0.263211 2:-22343630 MsG0280007885.01.T01:CDS
TTATGTTTCAATGATGATTC+TGG 0.299384 2:+22344722 None:intergenic
CCAATTACATTGTATCCTAT+TGG 0.304963 2:+22344935 None:intergenic
CCAATAGGATACAATGTAAT+TGG 0.307017 2:-22344935 MsG0280007885.01.T01:CDS
CTCCCTTTCGGCTTTCTAAC+TGG 0.309304 2:+22343947 None:intergenic
AATCCAACCACTTTCCATAT+TGG 0.332576 2:+22343689 None:intergenic
TGAGAACATTAGAAGAATTC+TGG 0.343239 2:+22345489 None:intergenic
TCTAAAGTTAACCTCAATCT+AGG 0.348348 2:-22344833 MsG0280007885.01.T01:CDS
ACGACTGGAGATTCCTCTTA+TGG 0.366120 2:-22345371 MsG0280007885.01.T01:CDS
AGATCAAGATATGTCGTTTA+CGG 0.368642 2:-22344795 MsG0280007885.01.T01:CDS
CAATGATGATTCTGGAAGAT+TGG 0.372954 2:+22344730 None:intergenic
AAGGATATGAGTAGCCAATA+TGG 0.377746 2:-22343703 MsG0280007885.01.T01:CDS
AATTTGCATAGGAAATCTTC+TGG 0.387773 2:-22345512 MsG0280007885.01.T01:CDS
TGTCATTATTATCAATTGAA+TGG 0.417904 2:+22345444 None:intergenic
TTGGAAACCAATGGCTTAGA+TGG 0.421919 2:+22345413 None:intergenic
GTTAACTTTAGAAACATCAT+TGG 0.426304 2:+22344844 None:intergenic
GCTTCTATGCCGTCCATAAG+AGG 0.428885 2:+22345358 None:intergenic
CATCATTGAAACATAAATCA+TGG 0.434027 2:-22344715 MsG0280007885.01.T01:CDS
GTAGCGAGTAATAAACAATA+TGG 0.440742 2:-22345139 MsG0280007885.01.T01:CDS
GAAAGGGATGAGAGATTCAA+TGG 0.441206 2:-22343743 MsG0280007885.01.T01:CDS
ATTTGTCTTAGGTGGCTGAT+TGG 0.442473 2:+22345544 None:intergenic
AGAATTCTTCTAATGTTCTC+AGG 0.451320 2:-22345487 MsG0280007885.01.T01:CDS
AGATAACTCTGCATCGGATT+CGG 0.451366 2:+22344162 None:intergenic
TTCTGAGCATTTGTCTTAGG+TGG 0.454477 2:+22345536 None:intergenic
GTAATTGGCGGCGATGCCTT+AGG 0.460790 2:-22344920 MsG0280007885.01.T01:CDS
GAAGGCTCAAGAACTGAGAT+TGG 0.465982 2:+22344405 None:intergenic
ACTCGCTACATTGACTTCAC+TGG 0.467621 2:+22345153 None:intergenic
GACAATACAAGGTCTCCAAT+AGG 0.471049 2:-22344950 MsG0280007885.01.T01:CDS
TGTCGAGGTGTCACAGAACC+TGG 0.473644 2:+22345037 None:intergenic
TGGAAAGTGGTTGGATTATG+AGG 0.473906 2:-22343683 MsG0280007885.01.T01:CDS
TCAAGAACTGAGATTGGACT+TGG 0.479255 2:+22344411 None:intergenic
CTGAGATTGGACTTGGTTGT+CGG 0.498616 2:+22344418 None:intergenic
TTTAGAAACATCATTGGAAG+AGG 0.499066 2:+22344850 None:intergenic
GAATACTAATGATCTTTCGT+TGG 0.507943 2:-22345005 MsG0280007885.01.T01:CDS
ATCAGTACCATCTAAGCCAT+TGG 0.511226 2:-22345420 MsG0280007885.01.T01:CDS
TCGCTCTGAAACCTCCCTTT+CGG 0.520590 2:+22343935 None:intergenic
TTAACAAGGAGCAGCAATCC+AGG 0.521935 2:-22345055 MsG0280007885.01.T01:CDS
ACGATGAATCGAGGATAGAA+AGG 0.525038 2:-22343913 MsG0280007885.01.T01:CDS
AACTCTGCATCGGATTCGGT+TGG 0.534201 2:+22344166 None:intergenic
CAGCTTCTTCAACATCAACT+AGG 0.535267 2:-22344135 MsG0280007885.01.T01:CDS
TCTGTTGCAATCCTAGATTG+AGG 0.548612 2:+22344822 None:intergenic
ATCATTGAAACATAAATCAT+GGG 0.554865 2:-22344714 MsG0280007885.01.T01:intron
CAAGGTAGAAAAGTTACGAC+TGG 0.558357 2:-22345386 MsG0280007885.01.T01:CDS
TTCAATGGTTGATGAGCTTG+TGG 0.564141 2:-22343728 MsG0280007885.01.T01:CDS
AACCAGTTAGAAAGCCGAAA+GGG 0.567053 2:-22343949 MsG0280007885.01.T01:CDS
TAGCCAATATGGAAAGTGGT+TGG 0.575562 2:-22343692 MsG0280007885.01.T01:CDS
CGAGTAATAAACAATATGGT+GGG 0.579550 2:-22345135 MsG0280007885.01.T01:CDS
ATTCAATCTCAAGAAATCCA+TGG 0.580104 2:-22344215 MsG0280007885.01.T01:CDS
TGCTTGATACAGACAATACA+AGG 0.581399 2:-22344961 MsG0280007885.01.T01:CDS
GGTTGCAAAAGATGTGTACA+AGG 0.582304 2:-22343779 MsG0280007885.01.T01:intron
TGAGTAGCCAATATGGAAAG+TGG 0.584195 2:-22343696 MsG0280007885.01.T01:CDS
ATAGGATACAATGTAATTGG+CGG 0.584996 2:-22344932 MsG0280007885.01.T01:CDS
ATGTGTGCAGGGTGATGAGA+TGG 0.589624 2:-22344460 MsG0280007885.01.T01:intron
GCGAGTAATAAACAATATGG+TGG 0.591690 2:-22345136 MsG0280007885.01.T01:CDS
CCATTGGTTTCCAACTCGCA+AGG 0.595241 2:-22345404 MsG0280007885.01.T01:CDS
TGAGTCAGATAACTCTGCAT+CGG 0.597249 2:+22344156 None:intergenic
GTATTCTTTCCACCTTGTCG+AGG 0.598057 2:+22345022 None:intergenic
GGTTGATGAGCTTGTGGACA+AGG 0.599314 2:-22343722 MsG0280007885.01.T01:CDS
CGTTCACTACACCATTAACA+AGG 0.603391 2:-22345069 MsG0280007885.01.T01:CDS
GCGGATGTTATCAGTACAGA+AGG 0.607854 2:-22344348 MsG0280007885.01.T01:CDS
CTGTTCTAAAAGGATACCTA+AGG 0.611780 2:+22344904 None:intergenic
CCTTGCGAGTTGGAAACCAA+TGG 0.620891 2:+22345404 None:intergenic
ACTATGATAAAGAAGCAGAC+AGG 0.622027 2:-22344112 MsG0280007885.01.T01:intron
GGACGGCATAGAAGCTCTAG+TGG 0.624669 2:-22345350 MsG0280007885.01.T01:intron
CAGTTAGAAAGCCGAAAGGG+AGG 0.631260 2:-22343946 MsG0280007885.01.T01:CDS
CTGGAGATTCCTCTTATGGA+CGG 0.637212 2:-22345367 MsG0280007885.01.T01:CDS
TTCAGAGCGACGATGAATCG+AGG 0.641324 2:-22343922 MsG0280007885.01.T01:CDS
CAACCAGTTAGAAAGCCGAA+AGG 0.642923 2:-22343950 MsG0280007885.01.T01:CDS
GACAGTTTGAACAGTAGCTG+TGG 0.647449 2:-22344773 MsG0280007885.01.T01:CDS
CTGTGAATCCTCGATGAGCG+CGG 0.653212 2:-22344367 MsG0280007885.01.T01:CDS
GATAACATCCGCGCTCATCG+AGG 0.659096 2:+22344359 None:intergenic
TGATTCTGGAAGATTGGTGA+AGG 0.660187 2:+22344736 None:intergenic
CAGCTTTCAACCGAAAACGA+AGG 0.668426 2:+22344387 None:intergenic
AAGTTGAGGGAATTGACAAG+TGG 0.671342 2:-22344881 MsG0280007885.01.T01:CDS
GGTTCTGTGACACCTCGACA+AGG 0.671483 2:-22345034 MsG0280007885.01.T01:CDS
TCTGTGACACCTCGACAAGG+TGG 0.707322 2:-22345031 MsG0280007885.01.T01:CDS
TGTCGGCAATTGAATGACAG+TGG 0.709419 2:+22344435 None:intergenic
TAATGGTGTAGTGAACGTGA+AGG 0.712147 2:+22345075 None:intergenic

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!! AGATAATAATCACTTAAATA+TGG + Chr2:22344457-22344476 None:intergenic 15.0%
!! TTGATCAAATATTTATTCTT+TGG - Chr2:22345500-22345519 MsG0280007885.01.T01:CDS 15.0%
!!! AATAATGACCTTTTATTAAT+TGG - Chr2:22344597-22344616 MsG0280007885.01.T01:intron 15.0%
!!! TTTTTACTGTATATATTTTC+AGG - Chr2:22344873-22344892 MsG0280007885.01.T01:CDS 15.0%
!! ATCATTGAAACATAAATCAT+GGG - Chr2:22344416-22344435 MsG0280007885.01.T01:CDS 20.0%
!! TGTCATTATTATCAATTGAA+TGG + Chr2:22343689-22343708 None:intergenic 20.0%
!!! ATAATTTTCCTAGAAAGAAA+CGG - Chr2:22343892-22343911 MsG0280007885.01.T01:CDS 20.0%
!!! GGTCATTATTCAATAATTTT+TGG + Chr2:22344587-22344606 None:intergenic 20.0%
! AAATGCCACCAATTAATAAA+AGG + Chr2:22344608-22344627 None:intergenic 25.0%
! AAATGGTGAAAAGAAAAGAT+TGG - Chr2:22345330-22345349 MsG0280007885.01.T01:intron 25.0%
! ATAGAATTTCATTTCGCTAT+CGG + Chr2:22343860-22343879 None:intergenic 25.0%
! CAATGTTGAGTTAATGTATA+TGG - Chr2:22345112-22345131 MsG0280007885.01.T01:CDS 25.0%
! CATCATTGAAACATAAATCA+TGG - Chr2:22344415-22344434 MsG0280007885.01.T01:CDS 25.0%
! CTTAAAAAGTTACTGTAGTT+TGG - Chr2:22344814-22344833 MsG0280007885.01.T01:CDS 25.0%
! GTATAATGTCATCAGTTTAA+TGG - Chr2:22344499-22344518 MsG0280007885.01.T01:intron 25.0%
! TTAATTTAATGACACATGAG+TGG - Chr2:22344844-22344863 MsG0280007885.01.T01:CDS 25.0%
! TTATGTTTCAATGATGATTC+TGG + Chr2:22344411-22344430 None:intergenic 25.0%
!! AATGACCTTTTATTAATTGG+TGG - Chr2:22344600-22344619 MsG0280007885.01.T01:intron 25.0%
!! ACATTTTCTATGATGAAGTT+TGG - Chr2:22345042-22345061 MsG0280007885.01.T01:CDS 25.0%
!! GTATTTTCAATGTCATCAAA+GGG + Chr2:22344565-22344584 None:intergenic 25.0%
!! GTTAACTTTAGAAACATCAT+TGG + Chr2:22344289-22344308 None:intergenic 25.0%
!!! AAGGAAATTTTGAGTTTGAA+AGG - Chr2:22345370-22345389 MsG0280007885.01.T01:CDS 25.0%
!!! AGGAAATTTTGAGTTTGAAA+GGG - Chr2:22345371-22345390 MsG0280007885.01.T01:CDS 25.0%
AATTTGCATAGGAAATCTTC+TGG - Chr2:22343618-22343637 MsG0280007885.01.T01:CDS 30.0%
AGAAGTGAAAAAGAAAGACA+TGG - Chr2:22344023-22344042 MsG0280007885.01.T01:intron 30.0%
AGAATTCTTCTAATGTTCTC+AGG - Chr2:22343643-22343662 MsG0280007885.01.T01:CDS 30.0%
ATAGGATACAATGTAATTGG+CGG - Chr2:22344198-22344217 MsG0280007885.01.T01:CDS 30.0%
ATGCTCAGAAAAATTTGCAT+AGG - Chr2:22343607-22343626 MsG0280007885.01.T01:CDS 30.0%
ATTCAATCTCAAGAAATCCA+TGG - Chr2:22344915-22344934 MsG0280007885.01.T01:CDS 30.0%
CCAATAGGATACAATGTAAT+TGG - Chr2:22344195-22344214 MsG0280007885.01.T01:CDS 30.0%
CCAATTACATTGTATCCTAT+TGG + Chr2:22344198-22344217 None:intergenic 30.0%
CCTTGAAAAAATTAAAGCCA+TGG + Chr2:22344935-22344954 None:intergenic 30.0%
CGAGTAATAAACAATATGGT+GGG - Chr2:22343995-22344014 MsG0280007885.01.T01:intron 30.0%
GAAATGAAATTCTATACACC+AGG - Chr2:22343865-22343884 MsG0280007885.01.T01:intron 30.0%
GAATACTAATGATCTTTCGT+TGG - Chr2:22344125-22344144 MsG0280007885.01.T01:CDS 30.0%
GTAGCGAGTAATAAACAATA+TGG - Chr2:22343991-22344010 MsG0280007885.01.T01:intron 30.0%
TCTAGGAAAATTATTTGTCC+TGG + Chr2:22343886-22343905 None:intergenic 30.0%
TGAGAACATTAGAAGAATTC+TGG + Chr2:22343644-22343663 None:intergenic 30.0%
TGAGTTAATGACTTACTCAA+TGG + Chr2:22344647-22344666 None:intergenic 30.0%
TTGAGATGTAGAAGTTATGT+TGG - Chr2:22345276-22345295 MsG0280007885.01.T01:intron 30.0%
TTTAGAAACATCATTGGAAG+AGG + Chr2:22344283-22344302 None:intergenic 30.0%
! AATGTATATGGATTTTCCCT+CGG - Chr2:22345124-22345143 MsG0280007885.01.T01:CDS 30.0%
! AGATCAAGATATGTCGTTTA+CGG - Chr2:22344335-22344354 MsG0280007885.01.T01:CDS 30.0%
! GGTATTTTCAATGTCATCAA+AGG + Chr2:22344566-22344585 None:intergenic 30.0%
! TCAAAGGGTATAAATTTAGC+AGG + Chr2:22344550-22344569 None:intergenic 30.0%
! TCCATTGCAAAATCTAAAGT+CGG - Chr2:22343807-22343826 MsG0280007885.01.T01:intron 30.0%
! TCTAAAGTTAACCTCAATCT+AGG - Chr2:22344297-22344316 MsG0280007885.01.T01:intron 30.0%
! TTTTTCTGAGCATTTGTCTT+AGG + Chr2:22343600-22343619 None:intergenic 30.0%
!!! CCATGGCTTTAATTTTTTCA+AGG - Chr2:22344932-22344951 MsG0280007885.01.T01:CDS 30.0%
AAGGATATGAGTAGCCAATA+TGG - Chr2:22345427-22345446 MsG0280007885.01.T01:CDS 35.0%
AATCCAACCACTTTCCATAT+TGG + Chr2:22345444-22345463 None:intergenic 35.0%
ACTATGATAAAGAAGCAGAC+AGG - Chr2:22345018-22345037 MsG0280007885.01.T01:CDS 35.0%
AGATGTAGAAGTTATGTTGG+TGG - Chr2:22345279-22345298 MsG0280007885.01.T01:intron 35.0%
AGGATACAAAATGTGGACAA+AGG - Chr2:22345302-22345321 MsG0280007885.01.T01:intron 35.0%
ATTAACTCAAAGATGTGTGC+AGG - Chr2:22344658-22344677 MsG0280007885.01.T01:intron 35.0%
GCGAGTAATAAACAATATGG+TGG - Chr2:22343994-22344013 MsG0280007885.01.T01:intron 35.0%
GGATACAAAATGTGGACAAA+GGG - Chr2:22345303-22345322 MsG0280007885.01.T01:intron 35.0%
TGATTGTGTGAGTGAATGTA+TGG - Chr2:22345251-22345270 MsG0280007885.01.T01:intron 35.0%
TGCTTGATACAGACAATACA+AGG - Chr2:22344169-22344188 MsG0280007885.01.T01:CDS 35.0%
TGGTAGATCATCAACTACAT+GGG - Chr2:22345062-22345081 MsG0280007885.01.T01:CDS 35.0%
TTAACTCAAAGATGTGTGCA+GGG - Chr2:22344659-22344678 MsG0280007885.01.T01:intron 35.0%
TTGGTAGATCATCAACTACA+TGG - Chr2:22345061-22345080 MsG0280007885.01.T01:CDS 35.0%
! CAATGATGATTCTGGAAGAT+TGG + Chr2:22344403-22344422 None:intergenic 35.0%
! TCCGACTTTAGATTTTGCAA+TGG + Chr2:22343811-22343830 None:intergenic 35.0%
!! CTGTTCTAAAAGGATACCTA+AGG + Chr2:22344229-22344248 None:intergenic 35.0%
!! GAGGTTGATGCTTATGAATT+TGG - Chr2:22345466-22345485 MsG0280007885.01.T01:CDS 35.0%
!!! CAGGTCTAGCTTTTTGTTTA+TGG + Chr2:22344531-22344550 None:intergenic 35.0%
!!! CCTTTTAGAACAGAAGTTGA+GGG - Chr2:22344236-22344255 MsG0280007885.01.T01:CDS 35.0%
!!! TCCTTTTAGAACAGAAGTTG+AGG - Chr2:22344235-22344254 MsG0280007885.01.T01:CDS 35.0%
AAAGAAACGGTCTACAGACA+AGG - Chr2:22343905-22343924 MsG0280007885.01.T01:CDS 40.0%
AACCAGTTAGAAAGCCGAAA+GGG - Chr2:22345181-22345200 MsG0280007885.01.T01:intron 40.0%
AAGTTGAGGGAATTGACAAG+TGG - Chr2:22344249-22344268 MsG0280007885.01.T01:intron 40.0%
ACGATGAATCGAGGATAGAA+AGG - Chr2:22345217-22345236 MsG0280007885.01.T01:intron 40.0%
AGATAACTCTGCATCGGATT+CGG + Chr2:22344971-22344990 None:intergenic 40.0%
ATCAGTACCATCTAAGCCAT+TGG - Chr2:22343710-22343729 MsG0280007885.01.T01:CDS 40.0%
ATTTGTCTTAGGTGGCTGAT+TGG + Chr2:22343589-22343608 None:intergenic 40.0%
CAAGGTAGAAAAGTTACGAC+TGG - Chr2:22343744-22343763 MsG0280007885.01.T01:CDS 40.0%
CAGCTTCTTCAACATCAACT+AGG - Chr2:22344995-22345014 MsG0280007885.01.T01:CDS 40.0%
CGTTCACTACACCATTAACA+AGG - Chr2:22344061-22344080 MsG0280007885.01.T01:intron 40.0%
GAAAGGGATGAGAGATTCAA+TGG - Chr2:22345387-22345406 MsG0280007885.01.T01:CDS 40.0%
GACAATACAAGGTCTCCAAT+AGG - Chr2:22344180-22344199 MsG0280007885.01.T01:CDS 40.0%
GGTTGCAAAAGATGTGTACA+AGG - Chr2:22345351-22345370 MsG0280007885.01.T01:CDS 40.0%
TAATGGTGTAGTGAACGTGA+AGG + Chr2:22344058-22344077 None:intergenic 40.0%
TAGCCAATATGGAAAGTGGT+TGG - Chr2:22345438-22345457 MsG0280007885.01.T01:CDS 40.0%
TCAAGAACTGAGATTGGACT+TGG + Chr2:22344722-22344741 None:intergenic 40.0%
TCTGTAGACCGTTTCTTTCT+AGG + Chr2:22343903-22343922 None:intergenic 40.0%
TCTGTTGCAATCCTAGATTG+AGG + Chr2:22344311-22344330 None:intergenic 40.0%
TGAGTAGCCAATATGGAAAG+TGG - Chr2:22345434-22345453 MsG0280007885.01.T01:CDS 40.0%
TGAGTCAGATAACTCTGCAT+CGG + Chr2:22344977-22344996 None:intergenic 40.0%
TGGAAAGTGGTTGGATTATG+AGG - Chr2:22345447-22345466 MsG0280007885.01.T01:CDS 40.0%
TGTGGACAAAGGGTTTGAAA+TGG - Chr2:22345313-22345332 MsG0280007885.01.T01:intron 40.0%
TTCTGAGCATTTGTCTTAGG+TGG + Chr2:22343597-22343616 None:intergenic 40.0%
TTGGAAACCAATGGCTTAGA+TGG + Chr2:22343720-22343739 None:intergenic 40.0%
TTGGTGGAGGATACAAAATG+TGG - Chr2:22345295-22345314 MsG0280007885.01.T01:intron 40.0%
! AACTTTTCTACCTTGCGAGT+TGG + Chr2:22343739-22343758 None:intergenic 40.0%
! AGTTCTTGAGCCTTCGTTTT+CGG - Chr2:22344733-22344752 MsG0280007885.01.T01:CDS 40.0%
! CCCTCAACTTCTGTTCTAAA+AGG + Chr2:22344239-22344258 None:intergenic 40.0%
! TGATTCTGGAAGATTGGTGA+AGG + Chr2:22344397-22344416 None:intergenic 40.0%
! TGTAGAAGTTATGTTGGTGG+AGG - Chr2:22345282-22345301 MsG0280007885.01.T01:intron 40.0%
!! TTCAATGGTTGATGAGCTTG+TGG - Chr2:22345402-22345421 MsG0280007885.01.T01:CDS 40.0%
ACGACTGGAGATTCCTCTTA+TGG - Chr2:22343759-22343778 MsG0280007885.01.T01:CDS 45.0%
AGTTCACAACCTCACGAGAT+CGG + Chr2:22345150-22345169 None:intergenic 45.0%
ATCGAAAAAGTCAGCTGTGG+AGG - Chr2:22343830-22343849 MsG0280007885.01.T01:intron 45.0%
CAACCAGTTAGAAAGCCGAA+AGG - Chr2:22345180-22345199 MsG0280007885.01.T01:intron 45.0%
CAGCTTTCAACCGAAAACGA+AGG + Chr2:22344746-22344765 None:intergenic 45.0%
CTGGAGATTCCTCTTATGGA+CGG - Chr2:22343763-22343782 MsG0280007885.01.T01:CDS 45.0%
GAAGGCTCAAGAACTGAGAT+TGG + Chr2:22344728-22344747 None:intergenic 45.0%
GACAGTTTGAACAGTAGCTG+TGG - Chr2:22344357-22344376 MsG0280007885.01.T01:CDS 45.0%
GCGGATGTTATCAGTACAGA+AGG - Chr2:22344782-22344801 MsG0280007885.01.T01:CDS 45.0%
GGATTGCTGCTCCTTGTTAA+TGG + Chr2:22344075-22344094 None:intergenic 45.0%
GTATTCTTTCCACCTTGTCG+AGG + Chr2:22344111-22344130 None:intergenic 45.0%
TGTCGGCAATTGAATGACAG+TGG + Chr2:22344698-22344717 None:intergenic 45.0%
TTAACAAGGAGCAGCAATCC+AGG - Chr2:22344075-22344094 MsG0280007885.01.T01:intron 45.0%
! CTGAGATTGGACTTGGTTGT+CGG + Chr2:22344715-22344734 None:intergenic 45.0%
!! ACTCGCTACATTGACTTCAC+TGG + Chr2:22343980-22343999 None:intergenic 45.0%
AACGGTCTACAGACAAGGAC+TGG - Chr2:22343910-22343929 MsG0280007885.01.T01:CDS 50.0%
ATGTGTGCAGGGTGATGAGA+TGG - Chr2:22344670-22344689 MsG0280007885.01.T01:intron 50.0%
CAGTTAGAAAGCCGAAAGGG+AGG - Chr2:22345184-22345203 MsG0280007885.01.T01:intron 50.0%
CGGATCGAAAAAGTCAGCTG+TGG - Chr2:22343827-22343846 MsG0280007885.01.T01:intron 50.0%
CTCCCTTTCGGCTTTCTAAC+TGG + Chr2:22345186-22345205 None:intergenic 50.0%
GCTTCTATGCCGTCCATAAG+AGG + Chr2:22343775-22343794 None:intergenic 50.0%
TTCAGAGCGACGATGAATCG+AGG - Chr2:22345208-22345227 MsG0280007885.01.T01:intron 50.0%
! AACTCTGCATCGGATTCGGT+TGG + Chr2:22344967-22344986 None:intergenic 50.0%
! CCTTGCGAGTTGGAAACCAA+TGG + Chr2:22343729-22343748 None:intergenic 50.0%
!! CCATTGGTTTCCAACTCGCA+AGG - Chr2:22343726-22343745 MsG0280007885.01.T01:CDS 50.0%
!! GGTTGATGAGCTTGTGGACA+AGG - Chr2:22345408-22345427 MsG0280007885.01.T01:CDS 50.0%
!! TCGCTCTGAAACCTCCCTTT+CGG + Chr2:22345198-22345217 None:intergenic 50.0%
CTGTGAATCCTCGATGAGCG+CGG - Chr2:22344763-22344782 MsG0280007885.01.T01:CDS 55.0%
GATAACATCCGCGCTCATCG+AGG + Chr2:22344774-22344793 None:intergenic 55.0%
GGTTCTGTGACACCTCGACA+AGG - Chr2:22344096-22344115 MsG0280007885.01.T01:intron 55.0%
GTAATTGGCGGCGATGCCTT+AGG - Chr2:22344210-22344229 MsG0280007885.01.T01:CDS 55.0%
TCTGTGACACCTCGACAAGG+TGG - Chr2:22344099-22344118 MsG0280007885.01.T01:intron 55.0%
! GGACGGCATAGAAGCTCTAG+TGG - Chr2:22343780-22343799 MsG0280007885.01.T01:intron 55.0%
! TGTCGAGGTGTCACAGAACC+TGG + Chr2:22344096-22344115 None:intergenic 55.0%
AACCTCACGAGATCGGCCGA+GGG + Chr2:22345143-22345162 None:intergenic 60.0%
CAACCTCACGAGATCGGCCG+AGG + Chr2:22345144-22345163 None:intergenic 65.0%
TTCCCTCGGCCGATCTCGTG+AGG - Chr2:22345138-22345157 MsG0280007885.01.T01:CDS 65.0%
Chromosome Type Strat End Strand Name
Chr2 gene 22343579 22345573 22343579 ID=MsG0280007885.01;Name=MsG0280007885.01
Chr2 mRNA 22343579 22345573 22343579 ID=MsG0280007885.01.T01;Parent=MsG0280007885.01;Name=MsG0280007885.01.T01;_AED=0.27;_eAED=0.27;_QI=0|0|0|1|0.6|0.83|6|0|435
Chr2 exon 22345351 22345573 22345351 ID=MsG0280007885.01.T01:exon:18316;Parent=MsG0280007885.01.T01
Chr2 exon 22344715 22345178 22344715 ID=MsG0280007885.01.T01:exon:18315;Parent=MsG0280007885.01.T01
Chr2 exon 22344317 22344472 22344317 ID=MsG0280007885.01.T01:exon:18314;Parent=MsG0280007885.01.T01
Chr2 exon 22344113 22344257 22344113 ID=MsG0280007885.01.T01:exon:18313;Parent=MsG0280007885.01.T01
Chr2 exon 22343885 22343992 22343885 ID=MsG0280007885.01.T01:exon:18312;Parent=MsG0280007885.01.T01
Chr2 exon 22343579 22343790 22343579 ID=MsG0280007885.01.T01:exon:18311;Parent=MsG0280007885.01.T01
Chr2 CDS 22345351 22345573 22345351 ID=MsG0280007885.01.T01:cds;Parent=MsG0280007885.01.T01
Chr2 CDS 22344715 22345178 22344715 ID=MsG0280007885.01.T01:cds;Parent=MsG0280007885.01.T01
Chr2 CDS 22344317 22344472 22344317 ID=MsG0280007885.01.T01:cds;Parent=MsG0280007885.01.T01
Chr2 CDS 22344113 22344257 22344113 ID=MsG0280007885.01.T01:cds;Parent=MsG0280007885.01.T01
Chr2 CDS 22343885 22343992 22343885 ID=MsG0280007885.01.T01:cds;Parent=MsG0280007885.01.T01
Chr2 CDS 22343579 22343790 22343579 ID=MsG0280007885.01.T01:cds;Parent=MsG0280007885.01.T01
Gene Sequence

>MsG0280007885.01.T01

ATGAAATCCAATCAGCCACCTAAGACAAATGCTCAGAAAAATTTGCATAGGAAATCTTCTGGCCAGAATTCTTCTAATGTTCTCAGGCAGAATAATCTGAAACAGAACCATTCAATTGATAATAATGACAGATCAGTACCATCTAAGCCATTGGTTTCCAACTCGCAAGGTAGAAAAGTTACGACTGGAGATTCCTCTTATGGACGGCATAGAAGCTCTAGTGAAGCCAGTGAAGTCAATGTAGCGAGTAATAAACAATATGGTGGGACTGAAGAAGTGAAAAAGAAAGACATGGATGTAGTTTCCTTCACGTTCACTACACCATTAACAAGGAGCAGCAATCCAGGTTCTGTGACACCTCGACAAGGTGGAAAGAATACTAATGATCTTTCGTTGGATCAACGCATTAAAAGAGTGTTGCTTGATACAGACAATACAAGGTCTCCAATAGGATACAATGTAATTGGCGGCGATGCCTTAGGTATCCTTTTAGAACAGAAGTTGAGGGAATTGACAAGTGGAGTTGAAACCTCTTCCAATGATGTTTCTAAAGTTAACCTCAATCTAGGATTGCAACAGAAGAAAGATCAAGATATGTCGTTTACGGACAGTTTGAACAGTAGCTGTGGTTCTAACATATCATCCTTCACCAATCTTCCAGAATCATCATTGAAACATAAATCATGGGGTGATGAGATGGAACCACTGTCATTCAATTGCCGACAACCAAGTCCAATCTCAGTTCTTGAGCCTTCGTTTTCGGTTGAAAGCTGTGAATCCTCGATGAGCGCGGATGTTATCAGTACAGAAGGTAAGTCATGCTTAAAAAGTTACTGTAGTTTGGAAGCAAGATGTTTTCATCTATTCAATCTCAAGAAATCCATGGCTTTAATTTTTTCAAGGAAGTTCTATCCAACCGAATCCGATGCAGAGTTATCTGACTCAGCTTCTTCAACATCAACTAGGACTATGATAAAGAAGCAGACAGGTTGTGAACTCTCATCTGTTCAACCAGTTAGAAAGCCGAAAGGGAGGTTTCAGAGCGACGATGAATCGAGGATAGAAAGGAAAGTTATATTTGATTGTGTGAGTGAATATGTGTACAAGGAAATTTTGAGTTTGAAAGGGATGAGAGATTCAATGGTTGATGAGCTTGTGGACAAGGATATGAGTAGCCAATATGGAAAGTGGTTGGATTATGAGGTTGATGCTTATGAATTTGGAGAAGAAGTTGTTGATCAAATATTTATTCTTTGGTTGATGATGTTGTCTATGAAATGTTGCAATTTTAAACACTTTGATGATTGA
Protein sequence

>MsG0280007885.01.T01

MKSNQPPKTNAQKNLHRKSSGQNSSNVLRQNNLKQNHSIDNNDRSVPSKPLVSNSQGRKVTTGDSSYGRHRSSSEASEVNVASNKQYGGTEEVKKKDMDVVSFTFTTPLTRSSNPGSVTPRQGGKNTNDLSLDQRIKRVLLDTDNTRSPIGYNVIGGDALGILLEQKLRELTSGVETSSNDVSKVNLNLGLQQKKDQDMSFTDSLNSSCGSNISSFTNLPESSLKHKSWGDEMEPLSFNCRQPSPISVLEPSFSVESCESSMSADVISTEGKSCLKSYCSLEARCFHLFNLKKSMALIFSRKFYPTESDAELSDSASSTSTRTMIKKQTGCELSSVQPVRKPKGRFQSDDESRIERKVIFDCVSEYVYKEILSLKGMRDSMVDELVDKDMSSQYGKWLDYEVDAYEFGEEVVDQIFILWLMMLSMKCCNFKHFDD*