Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MsG0280008006.01.T01 | XP_013463258.1 | 99.107 | 112 | 1 | 0 | 1 | 112 | 1 | 112 | 3.65E-76 | 230 |
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MsG0280008006.01.T01 | Q9LGE3 | 64.486 | 107 | 37 | 1 | 1 | 107 | 1 | 106 | 8.28E-48 | 151 |
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MsG0280008006.01.T01 | A0A072VGH9 | 99.107 | 112 | 1 | 0 | 1 | 112 | 1 | 112 | 1.74e-76 | 230 |
| Gene ID | Type | Classification |
|---|
| Gene ID | Type | Classification |
|---|
Co-expression Network
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|---|---|---|---|
| MsG0280008006.01 | MsG0380016300.01 | 0.800156 | 1.644622e-48 | 7.602955e-46 |
PPI
| Gene1 | Gene2 | Type |
|---|
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MsG0280008006.01.T01 | MTR_2g436880 | 99.107 | 112 | 1 | 0 | 1 | 112 | 1 | 112 | 4.42e-80 | 230 |
| MsG0280008006.01.T01 | MTR_4g119790 | 71.818 | 110 | 29 | 2 | 1 | 109 | 1 | 109 | 8.96e-50 | 154 |
| MsG0280008006.01.T01 | MTR_1g009900 | 60.909 | 110 | 39 | 2 | 1 | 109 | 1 | 107 | 3.14e-42 | 134 |
| MsG0280008006.01.T01 | MTR_2g096990 | 45.902 | 122 | 53 | 4 | 1 | 109 | 1 | 122 | 2.43e-31 | 107 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MsG0280008006.01.T01 | AT5G10625 | 57.658 | 111 | 46 | 1 | 1 | 110 | 1 | 111 | 2.99e-41 | 132 |
| MsG0280008006.01.T01 | AT5G24860 | 57.658 | 111 | 42 | 2 | 1 | 109 | 1 | 108 | 3.53e-40 | 129 |
| MsG0280008006.01.T01 | AT5G24860 | 57.658 | 111 | 42 | 2 | 1 | 109 | 37 | 144 | 8.33e-40 | 130 |
| MsG0280008006.01.T01 | AT5G24860 | 57.658 | 111 | 42 | 2 | 1 | 109 | 64 | 171 | 1.43e-39 | 130 |
| MsG0280008006.01.T01 | AT4G31380 | 55.645 | 124 | 41 | 4 | 1 | 110 | 1 | 124 | 2.93e-37 | 122 |
Find 47 sgRNAs with CRISPR-Local
Find 45 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| TCCAATAATGTCTGGGGTTT+GGG | 0.185327 | 2:-24207428 | None:intergenic |
| ACAAGAACTCCTTTGAAGTT+AGG | 0.274083 | 2:-24207120 | MsG0280008006.01.T01:CDS |
| TTCCAATAATGTCTGGGGTT+TGG | 0.287839 | 2:-24207429 | None:intergenic |
| CGGTGGAGCGTTTGTGGAAC+TGG | 0.360053 | 2:+24207215 | None:intergenic |
| GAGGGTGGCCGCCAAGGGTC+AGG | 0.369859 | 2:-24207358 | MsG0280008006.01.T01:CDS |
| CCCTTCCTATGGATTTCAAC+AGG | 0.377793 | 2:-24207180 | MsG0280008006.01.T01:CDS |
| TTGTGGAACTGGAGGAGGTC+AGG | 0.382460 | 2:+24207226 | None:intergenic |
| AGAGCGCAAGCTAAGTTCAT+TGG | 0.383371 | 2:-24207272 | MsG0280008006.01.T01:CDS |
| GAGCGCAAGCTAAGTTCATT+GGG | 0.385387 | 2:-24207271 | MsG0280008006.01.T01:CDS |
| TATACTATTCCAATAATGTC+TGG | 0.387499 | 2:-24207436 | None:intergenic |
| CAAGAACTCCTTTGAAGTTA+GGG | 0.391359 | 2:-24207119 | MsG0280008006.01.T01:CDS |
| GCGATGGAGGGTGGCCGCCA+AGG | 0.404816 | 2:-24207364 | MsG0280008006.01.T01:CDS |
| GATGAACGGTGGAGCGTTTG+TGG | 0.414552 | 2:+24207209 | None:intergenic |
| AGTGGATGGCGCGGAGGCGA+TGG | 0.428116 | 2:-24207380 | MsG0280008006.01.T01:CDS |
| TCATCTTATCTCCCTTCCTA+TGG | 0.432380 | 2:-24207191 | MsG0280008006.01.T01:CDS |
| GCGCTCTAAAACCGCGTAAG+AGG | 0.433490 | 2:+24207288 | None:intergenic |
| TGCGCCGTCCTCCTGACCCT+TGG | 0.458858 | 2:+24207347 | None:intergenic |
| GGATGGCGCGGAGGCGATGG+AGG | 0.464796 | 2:-24207377 | MsG0280008006.01.T01:CDS |
| ATACTATTCCAATAATGTCT+GGG | 0.489129 | 2:-24207435 | None:intergenic |
| AGGAGGACGGCGCAAGGTGT+TGG | 0.491858 | 2:-24207338 | MsG0280008006.01.T01:CDS |
| CGATGGAGGGTGGCCGCCAA+GGG | 0.496682 | 2:-24207363 | MsG0280008006.01.T01:CDS |
| CATCATTTCCCTAACTTCAA+AGG | 0.503094 | 2:+24207111 | None:intergenic |
| GCCGCCAAGGGTCAGGAGGA+CGG | 0.511142 | 2:-24207351 | MsG0280008006.01.T01:CDS |
| GGCGCGGAGGCGATGGAGGG+TGG | 0.534874 | 2:-24207373 | MsG0280008006.01.T01:CDS |
| ACCTGTTGAAATCCATAGGA+AGG | 0.541055 | 2:+24207179 | None:intergenic |
| AGCGTTTGTGGAACTGGAGG+AGG | 0.543767 | 2:+24207221 | None:intergenic |
| AGGGTCAGGAGGACGGCGCA+AGG | 0.551855 | 2:-24207344 | MsG0280008006.01.T01:CDS |
| GCCGTCCTCCTGACCCTTGG+CGG | 0.553255 | 2:+24207350 | None:intergenic |
| GGTGGCCGCCAAGGGTCAGG+AGG | 0.555196 | 2:-24207355 | MsG0280008006.01.T01:CDS |
| GAACGGTGTGGTGCGGCTAG+TGG | 0.557194 | 2:-24207398 | MsG0280008006.01.T01:CDS |
| CAAGCTAAGTTCATTGGGGT+GGG | 0.560305 | 2:-24207266 | MsG0280008006.01.T01:CDS |
| TGTGGAACTGGAGGAGGTCA+GGG | 0.564953 | 2:+24207227 | None:intergenic |
| GATGGCGCGGAGGCGATGGA+GGG | 0.565420 | 2:-24207376 | MsG0280008006.01.T01:CDS |
| GCAAGCTAAGTTCATTGGGG+TGG | 0.580281 | 2:-24207267 | MsG0280008006.01.T01:CDS |
| GGTGTGGTGCGGCTAGTGGA+TGG | 0.588257 | 2:-24207394 | MsG0280008006.01.T01:CDS |
| TACTATTCCAATAATGTCTG+GGG | 0.588950 | 2:-24207434 | None:intergenic |
| AACGATGTCATACATGTGCA+TGG | 0.603466 | 2:+24207150 | None:intergenic |
| CGAGATTATCACCTCTTACG+CGG | 0.604068 | 2:-24207299 | MsG0280008006.01.T01:CDS |
| TTGAACCTGTTGAAATCCAT+AGG | 0.604678 | 2:+24207175 | None:intergenic |
| GGTGATAATCTCGTTGCTTG+CGG | 0.604992 | 2:+24207309 | None:intergenic |
| CCTGTTGAAATCCATAGGAA+GGG | 0.632955 | 2:+24207180 | None:intergenic |
| GCGGCTAGTGGATGGCGCGG+AGG | 0.658493 | 2:-24207386 | MsG0280008006.01.T01:CDS |
| AGGAAGGGAGATAAGATGAA+CGG | 0.668997 | 2:+24207195 | None:intergenic |
| GGTGCGGCTAGTGGATGGCG+CGG | 0.672789 | 2:-24207389 | MsG0280008006.01.T01:CDS |
| AAGGGAGATAAGATGAACGG+TGG | 0.676928 | 2:+24207198 | None:intergenic |
| AGCGCAAGCTAAGTTCATTG+GGG | 0.682937 | 2:-24207270 | MsG0280008006.01.T01:CDS |
| TGGAGCGTTTGTGGAACTGG+AGG | 0.688009 | 2:+24207218 | None:intergenic |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|---|---|---|---|---|
| CATCATTTCCCTAACTTCAA+AGG | + | Chr2:24207418-24207437 | None:intergenic | 35.0% | |
| TTGAACCTGTTGAAATCCAT+AGG | + | Chr2:24207354-24207373 | None:intergenic | 35.0% | |
| !! | ACAAGAACTCCTTTGAAGTT+AGG | - | Chr2:24207406-24207425 | MsG0280008006.01.T01:CDS | 35.0% |
| !! | CAAGAACTCCTTTGAAGTTA+GGG | - | Chr2:24207407-24207426 | MsG0280008006.01.T01:CDS | 35.0% |
| AACGATGTCATACATGTGCA+TGG | + | Chr2:24207379-24207398 | None:intergenic | 40.0% | |
| ACCTGTTGAAATCCATAGGA+AGG | + | Chr2:24207350-24207369 | None:intergenic | 40.0% | |
| AGGAAGGGAGATAAGATGAA+CGG | + | Chr2:24207334-24207353 | None:intergenic | 40.0% | |
| CCTGTTGAAATCCATAGGAA+GGG | + | Chr2:24207349-24207368 | None:intergenic | 40.0% | |
| TCATCTTATCTCCCTTCCTA+TGG | - | Chr2:24207335-24207354 | MsG0280008006.01.T01:CDS | 40.0% | |
| AAGGGAGATAAGATGAACGG+TGG | + | Chr2:24207331-24207350 | None:intergenic | 45.0% | |
| AGAGCGCAAGCTAAGTTCAT+TGG | - | Chr2:24207254-24207273 | MsG0280008006.01.T01:CDS | 45.0% | |
| AGCGCAAGCTAAGTTCATTG+GGG | - | Chr2:24207256-24207275 | MsG0280008006.01.T01:CDS | 45.0% | |
| CAAGCTAAGTTCATTGGGGT+GGG | - | Chr2:24207260-24207279 | MsG0280008006.01.T01:CDS | 45.0% | |
| CCCTTCCTATGGATTTCAAC+AGG | - | Chr2:24207346-24207365 | MsG0280008006.01.T01:CDS | 45.0% | |
| CGAGATTATCACCTCTTACG+CGG | - | Chr2:24207227-24207246 | MsG0280008006.01.T01:CDS | 45.0% | |
| GAGCGCAAGCTAAGTTCATT+GGG | - | Chr2:24207255-24207274 | MsG0280008006.01.T01:CDS | 45.0% | |
| GGTGATAATCTCGTTGCTTG+CGG | + | Chr2:24207220-24207239 | None:intergenic | 45.0% | |
| !! | GGGGTTTGGGTTTTCAAGAA+CGG | - | Chr2:24207111-24207130 | MsG0280008006.01.T01:CDS | 45.0% |
| !! | TTGGGTTTTCAAGAACGGTG+TGG | - | Chr2:24207116-24207135 | MsG0280008006.01.T01:CDS | 45.0% |
| !! | TTTTCAAGAACGGTGTGGTG+CGG | - | Chr2:24207121-24207140 | MsG0280008006.01.T01:CDS | 45.0% |
| GCAAGCTAAGTTCATTGGGG+TGG | - | Chr2:24207259-24207278 | MsG0280008006.01.T01:CDS | 50.0% | |
| AGCGTTTGTGGAACTGGAGG+AGG | + | Chr2:24207308-24207327 | None:intergenic | 55.0% | |
| TGTGGAACTGGAGGAGGTCA+GGG | + | Chr2:24207302-24207321 | None:intergenic | 55.0% | |
| TTGTGGAACTGGAGGAGGTC+AGG | + | Chr2:24207303-24207322 | None:intergenic | 55.0% | |
| ! | GATGAACGGTGGAGCGTTTG+TGG | + | Chr2:24207320-24207339 | None:intergenic | 55.0% |
| ! | TGGAGCGTTTGTGGAACTGG+AGG | + | Chr2:24207311-24207330 | None:intergenic | 55.0% |
| !! | GCGCTCTAAAACCGCGTAAG+AGG | + | Chr2:24207241-24207260 | None:intergenic | 55.0% |
| ! | CGGTGGAGCGTTTGTGGAAC+TGG | + | Chr2:24207314-24207333 | None:intergenic | 60.0% |
| AGGAGGACGGCGCAAGGTGT+TGG | - | Chr2:24207188-24207207 | MsG0280008006.01.T01:CDS | 65.0% | |
| GAACGGTGTGGTGCGGCTAG+TGG | - | Chr2:24207128-24207147 | MsG0280008006.01.T01:CDS | 65.0% | |
| GGTGTGGTGCGGCTAGTGGA+TGG | - | Chr2:24207132-24207151 | MsG0280008006.01.T01:CDS | 65.0% | |
| AGGGTCAGGAGGACGGCGCA+AGG | - | Chr2:24207182-24207201 | MsG0280008006.01.T01:CDS | 70.0% | |
| AGTGGATGGCGCGGAGGCGA+TGG | - | Chr2:24207146-24207165 | MsG0280008006.01.T01:CDS | 70.0% | |
| CGATGGAGGGTGGCCGCCAA+GGG | - | Chr2:24207163-24207182 | MsG0280008006.01.T01:CDS | 70.0% | |
| GATGGCGCGGAGGCGATGGA+GGG | - | Chr2:24207150-24207169 | MsG0280008006.01.T01:CDS | 70.0% | |
| GCCGCCAAGGGTCAGGAGGA+CGG | - | Chr2:24207175-24207194 | MsG0280008006.01.T01:CDS | 70.0% | |
| GCCGTCCTCCTGACCCTTGG+CGG | + | Chr2:24207179-24207198 | None:intergenic | 70.0% | |
| TGCGCCGTCCTCCTGACCCT+TGG | + | Chr2:24207182-24207201 | None:intergenic | 70.0% | |
| ! | GGTGCGGCTAGTGGATGGCG+CGG | - | Chr2:24207137-24207156 | MsG0280008006.01.T01:CDS | 70.0% |
| GAGGGTGGCCGCCAAGGGTC+AGG | - | Chr2:24207168-24207187 | MsG0280008006.01.T01:CDS | 75.0% | |
| GCGATGGAGGGTGGCCGCCA+AGG | - | Chr2:24207162-24207181 | MsG0280008006.01.T01:CDS | 75.0% | |
| GGATGGCGCGGAGGCGATGG+AGG | - | Chr2:24207149-24207168 | MsG0280008006.01.T01:CDS | 75.0% | |
| GGTGGCCGCCAAGGGTCAGG+AGG | - | Chr2:24207171-24207190 | MsG0280008006.01.T01:CDS | 75.0% | |
| ! | GCGGCTAGTGGATGGCGCGG+AGG | - | Chr2:24207140-24207159 | MsG0280008006.01.T01:CDS | 75.0% |
| ! | GGCGCGGAGGCGATGGAGGG+TGG | - | Chr2:24207153-24207172 | MsG0280008006.01.T01:CDS | 80.0% |
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| Chr2 | gene | 24207105 | 24207443 | 24207105 | ID=MsG0280008006.01;Name=MsG0280008006.01 |
| Chr2 | mRNA | 24207105 | 24207443 | 24207105 | ID=MsG0280008006.01.T01;Parent=MsG0280008006.01;Name=MsG0280008006.01.T01;_AED=0.30;_eAED=0.30;_QI=0|-1|0|1|-1|1|1|0|112 |
| Chr2 | exon | 24207105 | 24207443 | 24207105 | ID=MsG0280008006.01.T01:exon:34725;Parent=MsG0280008006.01.T01 |
| Chr2 | CDS | 24207105 | 24207443 | 24207105 | ID=MsG0280008006.01.T01:cds;Parent=MsG0280008006.01.T01 |
| Gene Sequence |
| Protein sequence |
