Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MsG0380016300.01.T01 | XP_003601958.1 | 89.447 | 199 | 21 | 0 | 1 | 199 | 1 | 199 | 5.75E-130 | 373 |
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MsG0380016300.01.T01 | G7J3S4 | 89.447 | 199 | 21 | 0 | 1 | 199 | 1 | 199 | 2.74e-130 | 373 |
| Gene ID | Type | Classification |
|---|
| Gene ID | Type | Classification |
|---|
Co-expression Network
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|---|---|---|---|
| MsG0080047985.01 | MsG0380016300.01 | 0.800072 | 1.711053e-48 | 7.893439e-46 |
| MsG0080048134.01 | MsG0380016300.01 | 0.835533 | 1.587909e-56 | 1.947236e-53 |
| MsG0080048745.01 | MsG0380016300.01 | 0.819688 | 1.018255e-52 | 7.890538e-50 |
| MsG0080049046.01 | MsG0380016300.01 | 0.831079 | 2.045947e-55 | 2.196707e-52 |
| MsG0080049154.01 | MsG0380016300.01 | 0.816990 | 4.157754e-52 | 2.991197e-49 |
| MsG0180000551.01 | MsG0380016300.01 | 0.819410 | 1.178825e-52 | 9.064127e-50 |
| MsG0180001008.01 | MsG0380016300.01 | 0.824255 | 8.910521e-54 | 7.851007e-51 |
| MsG0180001027.01 | MsG0380016300.01 | 0.802718 | 4.912297e-49 | 2.423575e-46 |
| MsG0180001029.01 | MsG0380016300.01 | 0.812992 | 3.206149e-51 | 2.068579e-48 |
| MsG0180001915.01 | MsG0380016300.01 | 0.820604 | 6.284227e-53 | 4.994856e-50 |
| MsG0180002255.01 | MsG0380016300.01 | 0.809141 | 2.191540e-50 | 1.276576e-47 |
| MsG0180003249.01 | MsG0380016300.01 | 0.803022 | 4.250389e-49 | 2.113120e-46 |
| MsG0180003250.01 | MsG0380016300.01 | 0.807683 | 4.484599e-50 | 2.513756e-47 |
| MsG0180003320.01 | MsG0380016300.01 | 0.812546 | 4.014260e-51 | 2.559778e-48 |
| MsG0180003505.01 | MsG0380016300.01 | 0.804694 | 1.910062e-49 | 9.913785e-47 |
| MsG0180003849.01 | MsG0380016300.01 | 0.809582 | 1.762397e-50 | 1.038779e-47 |
| MsG0180004121.01 | MsG0380016300.01 | 0.802464 | 5.541431e-49 | 2.716272e-46 |
| MsG0180004232.01 | MsG0380016300.01 | 0.827697 | 1.355612e-54 | 1.318552e-51 |
| MsG0180004359.01 | MsG0380016300.01 | 0.825755 | 3.942748e-54 | 3.626385e-51 |
| MsG0180005281.01 | MsG0380016300.01 | 0.812003 | 5.275763e-51 | 3.315141e-48 |
| MsG0180005681.01 | MsG0380016300.01 | 0.805733 | 1.157811e-49 | 6.172467e-47 |
| MsG0280010936.01 | MsG0380016300.01 | 0.819550 | 1.095179e-52 | 8.453946e-50 |
| MsG0280011236.01 | MsG0380016300.01 | 0.823922 | 1.066976e-53 | 9.313896e-51 |
| MsG0280011267.01 | MsG0380016300.01 | 0.827033 | 1.955694e-54 | 1.865783e-51 |
| MsG0280011269.01 | MsG0380016300.01 | 0.814085 | 1.843514e-51 | 1.225179e-48 |
| MsG0280011292.01 | MsG0380016300.01 | 0.812617 | 3.873549e-51 | 2.474567e-48 |
| MsG0380011697.01 | MsG0380016300.01 | 0.805929 | 1.052704e-49 | 5.640214e-47 |
| MsG0380011968.01 | MsG0380016300.01 | 0.863329 | 2.690773e-64 | 8.127018e-61 |
| MsG0380011974.01 | MsG0380016300.01 | 0.832061 | 1.172128e-55 | 1.295618e-52 |
| MsG0380012889.01 | MsG0380016300.01 | 0.818901 | 1.538938e-52 | 1.166857e-49 |
| MsG0380013843.01 | MsG0380016300.01 | 0.802825 | 4.667769e-49 | 2.309370e-46 |
| MsG0380014344.01 | MsG0380016300.01 | 0.809090 | 2.247058e-50 | 1.307122e-47 |
| MsG0380014639.01 | MsG0380016300.01 | -0.802166 | 6.380797e-49 | 3.104094e-46 |
| MsG0380015323.01 | MsG0380016300.01 | 0.814562 | 1.446145e-51 | 9.736392e-49 |
| MsG0380015659.01 | MsG0380016300.01 | 0.822947 | 1.803463e-53 | 1.531484e-50 |
| MsG0380015660.01 | MsG0380016300.01 | 0.836681 | 8.115160e-57 | 1.030156e-53 |
| MsG0380015711.01 | MsG0380016300.01 | 0.838459 | 2.839257e-57 | 3.805489e-54 |
| MsG0380016299.01 | MsG0380016300.01 | 0.903225 | 4.322473e-79 | 6.339156e-75 |
| MsG0380016300.01 | MsG0380016301.01 | 0.865682 | 4.947925e-65 | 1.624052e-61 |
| MsG0380016300.01 | MsG0380016718.01 | -0.807845 | 4.142594e-50 | 2.331875e-47 |
| MsG0380016300.01 | MsG0380017168.01 | 0.806721 | 7.167524e-50 | 3.918697e-47 |
| MsG0380016300.01 | MsG0380017901.01 | 0.808187 | 3.503028e-50 | 1.989844e-47 |
| MsG0380016300.01 | MsG0480019146.01 | 0.852342 | 4.915266e-61 | 1.023942e-57 |
| MsG0380016300.01 | MsG0480019301.01 | 0.808029 | 3.785561e-50 | 2.141276e-47 |
| MsG0380016300.01 | MsG0480019346.01 | 0.800659 | 1.298900e-48 | 6.082257e-46 |
| MsG0380016300.01 | MsG0480019348.01 | 0.802262 | 6.097963e-49 | 2.973729e-46 |
| MsG0380016300.01 | MsG0480019666.01 | 0.811920 | 5.500269e-51 | 3.448250e-48 |
| MsG0380016300.01 | MsG0480019842.01 | 0.834266 | 3.309499e-56 | 3.907488e-53 |
| MsG0380016300.01 | MsG0480020904.01 | 0.856296 | 3.541215e-62 | 8.410890e-59 |
| MsG0380016300.01 | MsG0480021133.01 | 0.807191 | 5.702150e-50 | 3.155682e-47 |
| MsG0380016300.01 | MsG0480021360.01 | 0.853354 | 2.525236e-61 | 5.441145e-58 |
| MsG0380016300.01 | MsG0480021361.01 | 0.831108 | 2.011993e-55 | 2.162020e-52 |
| MsG0380016300.01 | MsG0480021573.01 | 0.816721 | 4.779086e-52 | 3.412684e-49 |
| MsG0380016300.01 | MsG0480022055.01 | 0.853246 | 2.712754e-61 | 5.823964e-58 |
| MsG0380016300.01 | MsG0480022428.01 | 0.821847 | 3.246907e-53 | 2.672375e-50 |
| MsG0380016300.01 | MsG0480023432.01 | 0.832178 | 1.096605e-55 | 1.216251e-52 |
| MsG0380016300.01 | MsG0580024087.01 | 0.849707 | 2.720941e-60 | 5.203234e-57 |
| MsG0380016300.01 | MsG0580024218.01 | 0.811425 | 7.045572e-51 | 4.359893e-48 |
| MsG0380016300.01 | MsG0580024219.01 | 0.806690 | 7.279008e-50 | 3.976239e-47 |
| MsG0380016300.01 | MsG0580024412.01 | 0.804148 | 2.482103e-49 | 1.270308e-46 |
| MsG0380016300.01 | MsG0580024932.01 | 0.801081 | 1.065401e-48 | 5.042578e-46 |
| MsG0380016300.01 | MsG0580025524.01 | 0.816545 | 5.234263e-52 | 3.719608e-49 |
| MsG0380016300.01 | MsG0580025550.01 | 0.839760 | 1.306464e-57 | 1.821995e-54 |
| MsG0380016300.01 | MsG0580025552.01 | 0.805754 | 1.145835e-49 | 6.111955e-47 |
| MsG0380016300.01 | MsG0580025586.01 | 0.808513 | 2.984674e-50 | 1.709925e-47 |
| MsG0380016300.01 | MsG0580025590.01 | 0.870762 | 1.141842e-66 | 4.493649e-63 |
| MsG0380016300.01 | MsG0580025961.01 | 0.815018 | 1.146004e-51 | 7.810906e-49 |
| MsG0380016300.01 | MsG0580026521.01 | 0.806811 | 6.860550e-50 | 3.759636e-47 |
| MsG0380016300.01 | MsG0580027751.01 | 0.812599 | 3.909731e-51 | 2.496401e-48 |
| MsG0380016300.01 | MsG0580027752.01 | 0.817329 | 3.489158e-52 | 2.533390e-49 |
| MsG0380016300.01 | MsG0580027753.01 | 0.804545 | 2.052318e-49 | 1.061063e-46 |
| MsG0380016300.01 | MsG0580029359.01 | 0.813726 | 2.211916e-51 | 1.455838e-48 |
| MsG0380016300.01 | MsG0580029445.01 | 0.804602 | 1.996914e-49 | 1.033970e-46 |
| MsG0380016300.01 | MsG0580029676.01 | 0.818960 | 1.492200e-52 | 1.133309e-49 |
| MsG0380016300.01 | MsG0580029937.01 | 0.807372 | 5.218944e-50 | 2.901888e-47 |
| MsG0380016300.01 | MsG0580029938.01 | 0.833978 | 3.909370e-56 | 4.576177e-53 |
| MsG0380016300.01 | MsG0680030296.01 | 0.800093 | 1.694337e-48 | 7.820334e-46 |
| MsG0380016300.01 | MsG0680030613.01 | 0.801780 | 7.659547e-49 | 3.689661e-46 |
| MsG0380016300.01 | MsG0680031139.01 | 0.810853 | 9.374200e-51 | 5.713885e-48 |
| MsG0380016300.01 | MsG0680031474.01 | 0.813954 | 1.970593e-51 | 1.304978e-48 |
| MsG0380016300.01 | MsG0680031684.01 | 0.818142 | 2.287657e-52 | 1.698434e-49 |
| MsG0380016300.01 | MsG0680032932.01 | 0.821974 | 3.034520e-53 | 2.506381e-50 |
| MsG0380016300.01 | MsG0680033015.01 | 0.803045 | 4.204389e-49 | 2.091525e-46 |
| MsG0380016300.01 | MsG0680034238.01 | 0.804707 | 1.898035e-49 | 9.854636e-47 |
| MsG0380016300.01 | MsG0680034348.01 | 0.806890 | 6.604418e-50 | 3.626494e-47 |
| MsG0380016300.01 | MsG0680034354.01 | 0.806230 | 9.099360e-50 | 4.913021e-47 |
| MsG0380016300.01 | MsG0680034356.01 | 0.819939 | 8.925148e-53 | 6.965137e-50 |
| MsG0380016300.01 | MsG0680034366.01 | 0.817377 | 3.403296e-52 | 2.474420e-49 |
| MsG0380016300.01 | MsG0680034418.01 | 0.821623 | 3.658418e-53 | 2.991740e-50 |
| MsG0380016300.01 | MsG0680034728.01 | 0.832933 | 7.125569e-56 | 8.082730e-53 |
| MsG0380016300.01 | MsG0680034974.01 | 0.820679 | 6.037535e-53 | 4.808968e-50 |
| MsG0380016300.01 | MsG0680034978.01 | 0.824309 | 8.654211e-54 | 7.636576e-51 |
| MsG0380016300.01 | MsG0680035584.01 | -0.808470 | 3.048146e-50 | 1.744394e-47 |
| MsG0380016300.01 | MsG0780036199.01 | 0.814375 | 1.591157e-51 | 1.065836e-48 |
| MsG0380016300.01 | MsG0780036208.01 | 0.820484 | 6.694452e-53 | 5.303656e-50 |
| MsG0380016300.01 | MsG0780036378.01 | 0.808537 | 2.949451e-50 | 1.690806e-47 |
| MsG0380016300.01 | MsG0780036472.01 | 0.807272 | 5.481405e-50 | 3.039975e-47 |
| MsG0380016300.01 | MsG0780036649.01 | 0.838063 | 3.593161e-57 | 4.757826e-54 |
| MsG0380016300.01 | MsG0780036839.01 | 0.867354 | 1.455841e-65 | 5.068083e-62 |
| MsG0380016300.01 | MsG0780036984.01 | 0.815072 | 1.114826e-51 | 7.609267e-49 |
| MsG0380016300.01 | MsG0780038313.01 | 0.800509 | 1.394263e-48 | 6.503631e-46 |
| MsG0380016300.01 | MsG0780038519.01 | 0.831557 | 1.560536e-55 | 1.699578e-52 |
| MsG0380016300.01 | MsG0780038818.01 | 0.859154 | 5.033324e-63 | 1.316317e-59 |
| MsG0380016300.01 | MsG0780038922.01 | 0.806350 | 8.584801e-50 | 4.649075e-47 |
| MsG0380016300.01 | MsG0780039237.01 | 0.828973 | 6.675256e-55 | 6.738500e-52 |
| MsG0380016300.01 | MsG0780039287.01 | -0.800897 | 1.161611e-48 | 5.472416e-46 |
| MsG0380016300.01 | MsG0780039373.01 | 0.808637 | 2.809095e-50 | 1.614429e-47 |
| MsG0380016300.01 | MsG0780039855.01 | 0.826482 | 2.647570e-54 | 2.485961e-51 |
| MsG0380016300.01 | MsG0780039883.01 | 0.820453 | 6.804818e-53 | 5.386805e-50 |
| MsG0380016300.01 | MsG0780039979.01 | 0.835387 | 1.728331e-56 | 2.110339e-53 |
| MsG0380016300.01 | MsG0780040497.01 | 0.826040 | 3.374521e-54 | 3.129025e-51 |
| MsG0380016300.01 | MsG0780040498.01 | 0.861706 | 8.498411e-64 | 2.425749e-60 |
| MsG0380016300.01 | MsG0780040598.01 | 0.813361 | 2.661438e-51 | 1.734463e-48 |
| MsG0380016300.01 | MsG0780040716.01 | 0.811201 | 7.879473e-51 | 4.847080e-48 |
| MsG0380016300.01 | MsG0780040784.01 | 0.802116 | 6.534485e-49 | 3.174700e-46 |
| MsG0380016300.01 | MsG0780040805.01 | 0.812330 | 4.475977e-51 | 2.837796e-48 |
| MsG0380016300.01 | MsG0780040831.01 | 0.804618 | 1.980978e-49 | 1.026141e-46 |
| MsG0380016300.01 | MsG0780041036.01 | 0.813549 | 2.419150e-51 | 1.584656e-48 |
| MsG0380016300.01 | MsG0780041248.01 | 0.801050 | 1.080961e-48 | 5.112317e-46 |
| MsG0380016300.01 | MsG0880042740.01 | 0.800503 | 1.398057e-48 | 6.520321e-46 |
| MsG0380016300.01 | MsG0880043212.01 | 0.870192 | 1.756993e-66 | 6.774111e-63 |
| MsG0380016300.01 | MsG0880043217.01 | 0.863331 | 2.686424e-64 | 8.114637e-61 |
| MsG0380016300.01 | MsG0880043906.01 | 0.806383 | 8.450335e-50 | 4.580042e-47 |
| MsG0380016300.01 | MsG0880045854.01 | 0.820764 | 5.771793e-53 | 4.608537e-50 |
| MsG0380016300.01 | MsG0880046271.01 | 0.836703 | 8.012257e-57 | 1.017772e-53 |
| MsG0380016300.01 | MsG0880046932.01 | 0.806070 | 9.835047e-50 | 5.288300e-47 |
| MsG0380016300.01 | MsG0880047383.01 | 0.882788 | 7.764128e-71 | 4.795025e-67 |
| MsG0380016300.01 | MsG0880047714.01 | 0.809003 | 2.345453e-50 | 1.361112e-47 |
| MsG0280006546.01 | MsG0380016300.01 | 0.843820 | 1.107605e-58 | 1.753837e-55 |
| MsG0280006559.01 | MsG0380016300.01 | 0.812169 | 4.852302e-51 | 3.062946e-48 |
| MsG0280006560.01 | MsG0380016300.01 | 0.809209 | 2.118568e-50 | 1.236350e-47 |
| MsG0280006575.01 | MsG0380016300.01 | 0.806601 | 7.599993e-50 | 4.142228e-47 |
| MsG0280006579.01 | MsG0380016300.01 | 0.816442 | 5.519116e-52 | 3.910916e-49 |
| MsG0280007405.01 | MsG0380016300.01 | 0.812233 | 4.700044e-51 | 2.972009e-48 |
| MsG0280007602.01 | MsG0380016300.01 | 0.808254 | 3.390340e-50 | 1.929232e-47 |
| MsG0280007678.01 | MsG0380016300.01 | 0.800406 | 1.462865e-48 | 6.806114e-46 |
| MsG0280007937.01 | MsG0380016300.01 | 0.840115 | 1.056002e-57 | 1.489315e-54 |
| MsG0280008006.01 | MsG0380016300.01 | 0.800156 | 1.644622e-48 | 7.602955e-46 |
| MsG0280009232.01 | MsG0380016300.01 | 0.820856 | 5.498803e-53 | 4.401945e-50 |
| MsG0280009657.01 | MsG0380016300.01 | 0.802424 | 5.647941e-49 | 2.765757e-46 |
| MsG0280010515.01 | MsG0380016300.01 | 0.802712 | 4.925019e-49 | 2.429471e-46 |
| MsG0280010539.01 | MsG0380016300.01 | 0.800356 | 1.497422e-48 | 6.958145e-46 |
PPI
| Gene1 | Gene2 | Type |
|---|
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MsG0380016300.01.T01 | MTR_3g087270 | 89.447 | 199 | 21 | 0 | 1 | 199 | 1 | 199 | 6.96e-134 | 373 |
| MsG0380016300.01.T01 | MTR_3g087280 | 87.940 | 199 | 24 | 0 | 1 | 199 | 1 | 199 | 8.12e-133 | 370 |
| MsG0380016300.01.T01 | MTR_3g087278 | 89.447 | 199 | 21 | 0 | 1 | 199 | 1 | 199 | 2.38e-132 | 369 |
| MsG0380016300.01.T01 | MTR_3g087325 | 88.945 | 199 | 22 | 0 | 1 | 199 | 1 | 199 | 3.86e-131 | 366 |
| MsG0380016300.01.T01 | MTR_3g087275 | 88.945 | 199 | 21 | 1 | 1 | 199 | 1 | 198 | 1.73e-129 | 362 |
| MsG0380016300.01.T01 | MTR_3g087330 | 85.930 | 199 | 28 | 0 | 1 | 199 | 1 | 199 | 1.15e-128 | 360 |
| MsG0380016300.01.T01 | MTR_3g087300 | 67.005 | 197 | 21 | 3 | 1 | 197 | 1 | 153 | 8.11e-87 | 254 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MsG0380016300.01.T01 | AT5G65030 | 30.612 | 196 | 113 | 6 | 11 | 187 | 10 | 201 | 8.23e-24 | 94.0 |
| MsG0380016300.01.T01 | AT5G10210 | 30.769 | 195 | 109 | 7 | 15 | 196 | 13 | 194 | 2.41e-23 | 92.4 |
Find 43 sgRNAs with CRISPR-Local
Find 52 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| CCACCAAATCAGTGCCCTTT+TGG | 0.235029 | 3:-81668218 | MsG0380016300.01.T01:CDS |
| CTCATGCCCTCAAGAGTTCT+TGG | 0.272286 | 3:-81668172 | MsG0380016300.01.T01:CDS |
| ATAAGAGGAGAATGAATAAT+TGG | 0.280000 | 3:-81667903 | MsG0380016300.01.T01:CDS |
| TTAATCTCCACTTCCACTTC+TGG | 0.281665 | 3:+81667964 | None:intergenic |
| ATGTTGGGGTCACAACTTAT+TGG | 0.325437 | 3:-81668102 | MsG0380016300.01.T01:CDS |
| CCACTAGGCTTTGTGTGTTA+AGG | 0.346972 | 3:+81668145 | None:intergenic |
| TGTTGGGGTCACAACTTATT+GGG | 0.372157 | 3:-81668101 | MsG0380016300.01.T01:CDS |
| ATTTGCGCTTGGTGCAGCTT+TGG | 0.382032 | 3:-81667815 | MsG0380016300.01.T01:CDS |
| ATCCCAAAAGGGCACTGATT+TGG | 0.392569 | 3:+81668215 | None:intergenic |
| TGCGCTTGGTGCAGCTTTGG+AGG | 0.430579 | 3:-81667812 | MsG0380016300.01.T01:CDS |
| ATTAAAAGACTCATCCCAAA+AGG | 0.445229 | 3:+81668203 | None:intergenic |
| GCAAGGCTGTATCTTCCAAC+AGG | 0.447411 | 3:-81668279 | MsG0380016300.01.T01:CDS |
| GATTCAAGAATCACCTTCCA+TGG | 0.467787 | 3:+81668066 | None:intergenic |
| AAAATGGTTGAAGATGGATT+TGG | 0.470551 | 3:-81668025 | MsG0380016300.01.T01:CDS |
| AGTAGCTTCGGTTGCTGAGA+TGG | 0.471131 | 3:-81667935 | MsG0380016300.01.T01:CDS |
| GATTAAAATAAAAGTAGCTT+CGG | 0.474275 | 3:-81667947 | MsG0380016300.01.T01:CDS |
| CTAAGGCAGAGGAAGATGTT+GGG | 0.479580 | 3:-81668117 | MsG0380016300.01.T01:CDS |
| CCAAAAGGGCACTGATTTGG+TGG | 0.481239 | 3:+81668218 | None:intergenic |
| AGTGGAAGTGATTGCAAAGA+GGG | 0.484026 | 3:-81668000 | MsG0380016300.01.T01:CDS |
| TTGGGAAAGGTGAGATTCCA+TGG | 0.488562 | 3:-81668083 | MsG0380016300.01.T01:CDS |
| AGATGGAGAAGCAAAATAAG+AGG | 0.509817 | 3:-81667918 | MsG0380016300.01.T01:CDS |
| CAACTGAAGTTTGCATATGC+AGG | 0.509989 | 3:+81668363 | None:intergenic |
| GCTAAGGCAGAGGAAGATGT+TGG | 0.514746 | 3:-81668118 | MsG0380016300.01.T01:CDS |
| TCTGCCTTAGCTCCACCACT+AGG | 0.532067 | 3:+81668130 | None:intergenic |
| ACGGCCATGACCATCGTGCT+TGG | 0.538014 | 3:-81667861 | MsG0380016300.01.T01:CDS |
| AAAGCCTAGTGGTGGAGCTA+AGG | 0.543884 | 3:-81668134 | MsG0380016300.01.T01:CDS |
| TTAAAAGACTCATCCCAAAA+GGG | 0.557578 | 3:+81668204 | None:intergenic |
| ATGAATAATTGGAATGAGTG+TGG | 0.557798 | 3:-81667892 | MsG0380016300.01.T01:CDS |
| GGGTCACAACTTATTGGGAA+AGG | 0.564555 | 3:-81668096 | MsG0380016300.01.T01:CDS |
| AGCACCAAGCACGATGGTCA+TGG | 0.566327 | 3:+81667857 | None:intergenic |
| CATAACGCCAGAAGTGGAAG+TGG | 0.570407 | 3:-81667971 | MsG0380016300.01.T01:CDS |
| TAGTGGTGGAGCTAAGGCAG+AGG | 0.576496 | 3:-81668128 | MsG0380016300.01.T01:CDS |
| GATTGTGATATATTTGCGCT+TGG | 0.580894 | 3:-81667826 | MsG0380016300.01.T01:CDS |
| TATGTTCATAACGCCAGAAG+TGG | 0.583659 | 3:-81667977 | MsG0380016300.01.T01:CDS |
| GATTCTTGAATCACAAAACA+TGG | 0.589945 | 3:-81668055 | MsG0380016300.01.T01:CDS |
| TTGAAGATGGATTTGGTGAG+TGG | 0.598106 | 3:-81668018 | MsG0380016300.01.T01:CDS |
| GAAAGGTGAGATTCCATGGA+AGG | 0.610143 | 3:-81668079 | MsG0380016300.01.T01:CDS |
| TAAGGCAGAGGAAGATGTTG+GGG | 0.620231 | 3:-81668116 | MsG0380016300.01.T01:CDS |
| TAACACACAAAGCCTAGTGG+TGG | 0.644101 | 3:-81668142 | MsG0380016300.01.T01:CDS |
| AAACGTTGAATTCATCAAGT+CGG | 0.660973 | 3:-81668316 | MsG0380016300.01.T01:CDS |
| CAGCGCAGCACCAAGCACGA+TGG | 0.681591 | 3:+81667851 | None:intergenic |
| CCTTAACACACAAAGCCTAG+TGG | 0.691369 | 3:-81668145 | MsG0380016300.01.T01:CDS |
| GAGTGGAAGTGATTGCAAAG+AGG | 0.703550 | 3:-81668001 | MsG0380016300.01.T01:CDS |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|---|---|---|---|---|
| !! | AAAACATGGAGTTAAAAAAA+TGG | - | Chr3:81668144-81668163 | MsG0380016300.01.T01:CDS | 20.0% |
| !! | GATTAAAATAAAAGTAGCTT+CGG | - | Chr3:81668238-81668257 | MsG0380016300.01.T01:CDS | 20.0% |
| ! | ATAAGAGGAGAATGAATAAT+TGG | - | Chr3:81668282-81668301 | MsG0380016300.01.T01:CDS | 25.0% |
| ! | GTTAAAAAAATGGTTGAAGA+TGG | - | Chr3:81668154-81668173 | MsG0380016300.01.T01:CDS | 25.0% |
| AAACGTTGAATTCATCAAGT+CGG | - | Chr3:81667869-81667888 | MsG0380016300.01.T01:CDS | 30.0% | |
| ATGAATAATTGGAATGAGTG+TGG | - | Chr3:81668293-81668312 | MsG0380016300.01.T01:CDS | 30.0% | |
| ATTAAAAGACTCATCCCAAA+AGG | + | Chr3:81667985-81668004 | None:intergenic | 30.0% | |
| GATTCTTGAATCACAAAACA+TGG | - | Chr3:81668130-81668149 | MsG0380016300.01.T01:CDS | 30.0% | |
| TTAAAAGACTCATCCCAAAA+GGG | + | Chr3:81667984-81668003 | None:intergenic | 30.0% | |
| ! | AAAATGGTTGAAGATGGATT+TGG | - | Chr3:81668160-81668179 | MsG0380016300.01.T01:CDS | 30.0% |
| !! | ATTCTTTTCTTGTTTCCTGT+TGG | + | Chr3:81667924-81667943 | None:intergenic | 30.0% |
| AATGAGTGTGGATGCAAAAA+CGG | - | Chr3:81668305-81668324 | MsG0380016300.01.T01:CDS | 35.0% | |
| AGATGGAGAAGCAAAATAAG+AGG | - | Chr3:81668267-81668286 | MsG0380016300.01.T01:CDS | 35.0% | |
| GATTGTGATATATTTGCGCT+TGG | - | Chr3:81668359-81668378 | MsG0380016300.01.T01:CDS | 35.0% | |
| AGTGGAAGTGATTGCAAAGA+GGG | - | Chr3:81668185-81668204 | MsG0380016300.01.T01:CDS | 40.0% | |
| ATGTTGGGGTCACAACTTAT+TGG | - | Chr3:81668083-81668102 | MsG0380016300.01.T01:CDS | 40.0% | |
| CAACTGAAGTTTGCATATGC+AGG | + | Chr3:81667825-81667844 | None:intergenic | 40.0% | |
| CGGCAAAAAACATGTTTGCA+AGG | - | Chr3:81667889-81667908 | MsG0380016300.01.T01:CDS | 40.0% | |
| GATTCAAGAATCACCTTCCA+TGG | + | Chr3:81668122-81668141 | None:intergenic | 40.0% | |
| TATGTTCATAACGCCAGAAG+TGG | - | Chr3:81668208-81668227 | MsG0380016300.01.T01:CDS | 40.0% | |
| TGTTGGGGTCACAACTTATT+GGG | - | Chr3:81668084-81668103 | MsG0380016300.01.T01:CDS | 40.0% | |
| TTAATCTCCACTTCCACTTC+TGG | + | Chr3:81668224-81668243 | None:intergenic | 40.0% | |
| ! | AGGTTTTCCAAGAACTCTTG+AGG | + | Chr3:81668023-81668042 | None:intergenic | 40.0% |
| ! | GGTTTTCCAAGAACTCTTGA+GGG | + | Chr3:81668022-81668041 | None:intergenic | 40.0% |
| !! | TTGAAGATGGATTTGGTGAG+TGG | - | Chr3:81668167-81668186 | MsG0380016300.01.T01:CDS | 40.0% |
| CCTTAACACACAAAGCCTAG+TGG | - | Chr3:81668040-81668059 | MsG0380016300.01.T01:CDS | 45.0% | |
| CTAAGGCAGAGGAAGATGTT+GGG | - | Chr3:81668068-81668087 | MsG0380016300.01.T01:CDS | 45.0% | |
| GAAAGGTGAGATTCCATGGA+AGG | - | Chr3:81668106-81668125 | MsG0380016300.01.T01:CDS | 45.0% | |
| GAGTGGAAGTGATTGCAAAG+AGG | - | Chr3:81668184-81668203 | MsG0380016300.01.T01:CDS | 45.0% | |
| GGGTCACAACTTATTGGGAA+AGG | - | Chr3:81668089-81668108 | MsG0380016300.01.T01:CDS | 45.0% | |
| TAACACACAAAGCCTAGTGG+TGG | - | Chr3:81668043-81668062 | MsG0380016300.01.T01:CDS | 45.0% | |
| TAAGGCAGAGGAAGATGTTG+GGG | - | Chr3:81668069-81668088 | MsG0380016300.01.T01:CDS | 45.0% | |
| TTGGGAAAGGTGAGATTCCA+TGG | - | Chr3:81668102-81668121 | MsG0380016300.01.T01:CDS | 45.0% | |
| ! | CACCAAATCAGTGCCCTTTT+GGG | - | Chr3:81667968-81667987 | MsG0380016300.01.T01:CDS | 45.0% |
| ! | CCACTAGGCTTTGTGTGTTA+AGG | + | Chr3:81668043-81668062 | None:intergenic | 45.0% |
| ! | GCATATGCAGGTGTTTTCGA+TGG | + | Chr3:81667813-81667832 | None:intergenic | 45.0% |
| !! | ATCCCAAAAGGGCACTGATT+TGG | + | Chr3:81667973-81667992 | None:intergenic | 45.0% |
| AGTAGCTTCGGTTGCTGAGA+TGG | - | Chr3:81668250-81668269 | MsG0380016300.01.T01:CDS | 50.0% | |
| CATAACGCCAGAAGTGGAAG+TGG | - | Chr3:81668214-81668233 | MsG0380016300.01.T01:CDS | 50.0% | |
| CTCATGCCCTCAAGAGTTCT+TGG | - | Chr3:81668013-81668032 | MsG0380016300.01.T01:CDS | 50.0% | |
| GCAAGGCTGTATCTTCCAAC+AGG | - | Chr3:81667906-81667925 | MsG0380016300.01.T01:CDS | 50.0% | |
| GCTAAGGCAGAGGAAGATGT+TGG | - | Chr3:81668067-81668086 | MsG0380016300.01.T01:CDS | 50.0% | |
| ! | AAAGCCTAGTGGTGGAGCTA+AGG | - | Chr3:81668051-81668070 | MsG0380016300.01.T01:CDS | 50.0% |
| ! | CCACCAAATCAGTGCCCTTT+TGG | - | Chr3:81667967-81667986 | MsG0380016300.01.T01:CDS | 50.0% |
| !! | ATTTGCGCTTGGTGCAGCTT+TGG | - | Chr3:81668370-81668389 | MsG0380016300.01.T01:CDS | 50.0% |
| !! | CCAAAAGGGCACTGATTTGG+TGG | + | Chr3:81667970-81667989 | None:intergenic | 50.0% |
| AGCACCAAGCACGATGGTCA+TGG | + | Chr3:81668331-81668350 | None:intergenic | 55.0% | |
| TCTGCCTTAGCTCCACCACT+AGG | + | Chr3:81668058-81668077 | None:intergenic | 55.0% | |
| ! | TAGTGGTGGAGCTAAGGCAG+AGG | - | Chr3:81668057-81668076 | MsG0380016300.01.T01:CDS | 55.0% |
| ACGGCCATGACCATCGTGCT+TGG | - | Chr3:81668324-81668343 | MsG0380016300.01.T01:CDS | 60.0% | |
| !! | TGCGCTTGGTGCAGCTTTGG+AGG | - | Chr3:81668373-81668392 | MsG0380016300.01.T01:CDS | 60.0% |
| CAGCGCAGCACCAAGCACGA+TGG | + | Chr3:81668337-81668356 | None:intergenic | 65.0% |
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| Chr3 | gene | 81667804 | 81668403 | 81667804 | ID=MsG0380016300.01;Name=MsG0380016300.01 |
| Chr3 | mRNA | 81667804 | 81668403 | 81667804 | ID=MsG0380016300.01.T01;Parent=MsG0380016300.01;Name=MsG0380016300.01.T01;_AED=0.48;_eAED=0.48;_QI=0|-1|0|1|-1|1|1|0|199 |
| Chr3 | exon | 81667804 | 81668403 | 81667804 | ID=MsG0380016300.01.T01:exon:33053;Parent=MsG0380016300.01.T01 |
| Chr3 | CDS | 81667804 | 81668403 | 81667804 | ID=MsG0380016300.01.T01:cds;Parent=MsG0380016300.01.T01 |
| Gene Sequence |
| Protein sequence |
