AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar ZhongmuNo.1 / MsG0280008050.01

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MsG0280008050.01.T01 MTR_2g088950 92.557 309 20 1 19 324 18 326 0.0 593
MsG0280008050.01.T01 MTR_2g437670 92.233 309 21 1 19 324 18 326 0.0 592
MsG0280008050.01.T01 MTR_8g080020 41.228 228 114 6 101 322 77 290 4.53e-50 169
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MsG0280008050.01.T01 AT1G13810 49.286 280 132 4 45 319 6 280 2.77e-83 254
MsG0280008050.01.T01 AT1G67660 40.088 227 120 5 101 322 69 284 2.75e-50 169
MsG0280008050.01.T01 AT1G67660 40.088 227 120 5 101 322 98 313 5.12e-50 169
MsG0280008050.01.T01 AT1G67660 40.088 227 120 5 101 322 119 334 6.59e-50 170

Find 74 sgRNAs with CRISPR-Local

Find 187 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
AAATAGCACTTTCTGATTTC+TGG 0.099254 2:-24853482 MsG0280008050.01.T01:CDS
TCCTCAAGCTCAGGGTTTAA+TGG 0.201190 2:-24853604 MsG0280008050.01.T01:CDS
TTGCTGCGGCCATTGGGTTT+TGG 0.268639 2:-24853953 MsG0280008050.01.T01:CDS
GCACGGAAGTAGCTATTATA+AGG 0.299779 2:-24854180 MsG0280008050.01.T01:CDS
GTGGCAAGGTTTCCAGAAAA+TGG 0.312892 2:+24853882 None:intergenic
GATAACCCTTCTAAAAGATT+TGG 0.315772 2:-24854101 MsG0280008050.01.T01:CDS
GCAGCGAGCCAACTGTCTTC+TGG 0.320039 2:+24853759 None:intergenic
CAGGGTTTAATGGAGATTCT+AGG 0.359038 2:-24853594 MsG0280008050.01.T01:CDS
TTAATGGCAGTAGTCTATTC+AGG 0.360819 2:-24853539 MsG0280008050.01.T01:CDS
CTGCTATACAACTCTCTTGC+CGG 0.383061 2:+24853444 None:intergenic
TCACTATATTCCTCAAGCTC+AGG 0.390430 2:-24853613 MsG0280008050.01.T01:CDS
ATATCGTGATCCAGAGTATT+GGG 0.405244 2:-24853514 MsG0280008050.01.T01:CDS
AACCATGTTATCTCTACTTC+TGG 0.420744 2:+24856412 None:intergenic
GAGGTTAAGTGTCCGTATTT+CGG 0.433001 2:-24853678 MsG0280008050.01.T01:CDS
TTTGCCTTCTCGTGGTGTAC+TGG 0.440286 2:-24853700 MsG0280008050.01.T01:CDS
ATAACATGGTTGCTTCAAAT+AGG 0.444253 2:-24856400 MsG0280008050.01.T01:CDS
TCCAACACTGGTTTAAGAAC+TGG 0.447512 2:-24854013 MsG0280008050.01.T01:CDS
TTCATTACATCCCAATACTC+TGG 0.450502 2:+24853504 None:intergenic
CTCATGTCTCCACCGAAATA+CGG 0.455722 2:+24853666 None:intergenic
TCACGACATAATTCATGTCT+AGG 0.465169 2:+24853372 None:intergenic
CAGTTGGCTCGCTGCTTCAC+CGG 0.467708 2:-24853751 MsG0280008050.01.T01:CDS
TTAAGAACTGGCAGACACTT+AGG 0.467984 2:-24854001 MsG0280008050.01.T01:CDS
GCTTGAGGAATATAGTGAAC+TGG 0.474268 2:+24853618 None:intergenic
CACTATATTCCTCAAGCTCA+GGG 0.475332 2:-24853612 MsG0280008050.01.T01:CDS
CACCGGATGGTACAATTGAT+AGG 0.477590 2:-24853734 MsG0280008050.01.T01:CDS
CAGCTATGGTTAGAGAAGAT+TGG 0.482961 2:-24853915 MsG0280008050.01.T01:CDS
CCTTCTAAAAGATTTGGTAA+TGG 0.491879 2:-24854095 MsG0280008050.01.T01:CDS
CTGGAAACCTTGCCACATGT+TGG 0.492433 2:-24853875 MsG0280008050.01.T01:CDS
AAAAGATTTGGTAATGGTGG+TGG 0.492454 2:-24854089 MsG0280008050.01.T01:CDS
AAGCACCATGCCTCCAACAC+TGG 0.492645 2:-24854025 MsG0280008050.01.T01:CDS
GCCAGTTCTTAAACCAGTGT+TGG 0.493725 2:+24854012 None:intergenic
CACTTCTGTGGAAACACACT+CGG 0.497597 2:-24854054 MsG0280008050.01.T01:CDS
TATATCGTGATCCAGAGTAT+TGG 0.502102 2:-24853515 MsG0280008050.01.T01:CDS
ACTTAGGAAACAAAAGTTAA+CGG 0.509320 2:-24853985 MsG0280008050.01.T01:CDS
TCCATTAAACCCTGAGCTTG+AGG 0.518801 2:+24853603 None:intergenic
TTAAACCAGTGTTGGAGGCA+TGG 0.521048 2:+24854020 None:intergenic
AGTTCTTAAACCAGTGTTGG+AGG 0.524451 2:+24854015 None:intergenic
CACGACATAATTCATGTCTA+GGG 0.525168 2:+24853373 None:intergenic
GAAAGATACAAACTGATGAC+TGG 0.527655 2:-24853825 MsG0280008050.01.T01:CDS
CACGGAAGTAGCTATTATAA+GGG 0.530537 2:-24854179 MsG0280008050.01.T01:CDS
TGGAGATTCTAGGAAGGGAC+TGG 0.536702 2:-24853584 MsG0280008050.01.T01:CDS
ATCCTATCAATTGTACCATC+CGG 0.538571 2:+24853732 None:intergenic
CTGGTGGAAGCATGTTCAGC+CGG 0.539322 2:-24853463 MsG0280008050.01.T01:CDS
TAGCACTTTCTGATTTCTGG+TGG 0.540531 2:-24853479 MsG0280008050.01.T01:CDS
TTTCGGTGGAGACATGAGTA+AGG 0.541341 2:-24853661 MsG0280008050.01.T01:CDS
GCCTTCTCGTGGTGTACTGG+AGG 0.544719 2:-24853697 MsG0280008050.01.T01:CDS
TCTAAAAGATTTGGTAATGG+TGG 0.545520 2:-24854092 MsG0280008050.01.T01:CDS
TCAAATAGGAGTGAAACTGC+TGG 0.551496 2:-24856386 MsG0280008050.01.T01:CDS
GATTCTAGGAAGGGACTGGA+TGG 0.553292 2:-24853580 MsG0280008050.01.T01:CDS
TGGCTCGCTGCTTCACCGGA+TGG 0.553739 2:-24853747 MsG0280008050.01.T01:CDS
TAGAAGGGTTATCAGGAGCA+TGG 0.557024 2:+24854111 None:intergenic
TTTAATGGAGATTCTAGGAA+GGG 0.576159 2:-24853589 MsG0280008050.01.T01:CDS
TTTGATATTGCTCCAACATG+TGG 0.576521 2:+24853863 None:intergenic
GGATGGTACAATTGATAGGA+TGG 0.583652 2:-24853730 MsG0280008050.01.T01:CDS
ACATGAGTAAGGCTTTGCCT+TGG 0.583739 2:-24853650 MsG0280008050.01.T01:CDS
CAAATAGGAGTGAAACTGCT+GGG 0.584854 2:-24856385 MsG0280008050.01.T01:intron
TATTACTCAAACACGCAACA+AGG 0.585527 2:+24854153 None:intergenic
TATTGCTCCAACATGTGGCA+AGG 0.593904 2:+24853868 None:intergenic
CATGTTGAAATTCACTTCTG+TGG 0.593910 2:-24854066 MsG0280008050.01.T01:CDS
GTTTAATGGAGATTCTAGGA+AGG 0.596678 2:-24853590 MsG0280008050.01.T01:CDS
GTTAAGTGTCCGTATTTCGG+TGG 0.610192 2:-24853675 MsG0280008050.01.T01:CDS
GTTCAAAACCAGAAGACAGT+TGG 0.615705 2:-24853767 MsG0280008050.01.T01:CDS
GTAAGCGAAGATCTCAGCTA+TGG 0.615952 2:-24853929 MsG0280008050.01.T01:CDS
TGTGTGCAACATTTCAAGCA+CGG 0.618164 2:-24854197 MsG0280008050.01.T01:CDS
GTATATGATTTGCCTTCTCG+TGG 0.621951 2:-24853708 MsG0280008050.01.T01:CDS
CATAATTCATGTCTAGGGGA+TGG 0.623639 2:+24853378 None:intergenic
GCAAGAGAGTTGTATAGCAG+TGG 0.624726 2:-24853441 MsG0280008050.01.T01:CDS
TCGCTTACGCCAAAACCCAA+TGG 0.629109 2:+24853944 None:intergenic
TACCAGAAGTAGAGATAACA+TGG 0.631023 2:-24856414 MsG0280008050.01.T01:CDS
ACCTCCAGTACACCACGAGA+AGG 0.645087 2:+24853696 None:intergenic
TGAACTGGAATTCGATACCA+AGG 0.651798 2:+24853633 None:intergenic
ACGACATAATTCATGTCTAG+GGG 0.664530 2:+24853374 None:intergenic
ATTACTCAAACACGCAACAA+GGG 0.669522 2:+24854154 None:intergenic
ATGTTGGAGCAATATCAAAG+AGG 0.704264 2:-24853859 MsG0280008050.01.T01:CDS

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!!! ATTTTTCTTTTAATATATAG+AGG - Chr2:24854139-24854158 MsG0280008050.01.T01:CDS 10.0%
!! AAATTATTTAAGGGTTTATA+TGG + Chr2:24855100-24855119 None:intergenic 15.0%
!! ACTGTTAGATAAATTATTTA+AGG + Chr2:24855110-24855129 None:intergenic 15.0%
!! CAAAATATACAAAGAAATAT+AGG - Chr2:24854006-24854025 MsG0280008050.01.T01:CDS 15.0%
!! CTGTTAGATAAATTATTTAA+GGG + Chr2:24855109-24855128 None:intergenic 15.0%
!! TTAGTTATAAGCTAAAAAAT+TGG + Chr2:24854232-24854251 None:intergenic 15.0%
!! TTGTTATCAAACATATATAT+AGG + Chr2:24854272-24854291 None:intergenic 15.0%
!!! CTATATTTCTTTGTATATTT+TGG + Chr2:24854008-24854027 None:intergenic 15.0%
!!! GTATTTTTTATTAGTCATTT+TGG + Chr2:24853920-24853939 None:intergenic 15.0%
!!! TTTACTTTTGTTAACTTATT+TGG - Chr2:24854423-24854442 MsG0280008050.01.T01:intron 15.0%
!! AAAAGCCAAAAGAAAAAAAA+AGG - Chr2:24853511-24853530 MsG0280008050.01.T01:CDS 20.0%
!! AATCAAAAGTTTATCATCAT+TGG + Chr2:24854879-24854898 None:intergenic 20.0%
!! AGAAACATTTAATGTTTCAT+TGG - Chr2:24855276-24855295 MsG0280008050.01.T01:intron 20.0%
!! ATCAAAAGTTTATCATCATT+GGG + Chr2:24854878-24854897 None:intergenic 20.0%
!! ATGTTTGAATACATATATGT+TGG - Chr2:24853684-24853703 MsG0280008050.01.T01:CDS 20.0%
!! TCCTACTTTACTTATAATTT+GGG + Chr2:24855254-24855273 None:intergenic 20.0%
!! TTCCTACTTTACTTATAATT+TGG + Chr2:24855255-24855274 None:intergenic 20.0%
!!! TGTATTTTATACAACTTCTT+CGG + Chr2:24854516-24854535 None:intergenic 20.0%
!!! TTATTTGAACAACTCTTTTT+GGG - Chr2:24853966-24853985 MsG0280008050.01.T01:CDS 20.0%
! ACCAATACTATAGATAATCA+TGG + Chr2:24855450-24855469 None:intergenic 25.0%
! ACCATGATTATCTATAGTAT+TGG - Chr2:24855446-24855465 MsG0280008050.01.T01:intron 25.0%
! ACTTAGGAAACAAAAGTTAA+CGG - Chr2:24855793-24855812 MsG0280008050.01.T01:intron 25.0%
! AGAGAGAAAAAGTAAAAACA+CGG - Chr2:24854057-24854076 MsG0280008050.01.T01:CDS 25.0%
! AGCGACTTATACTTATAATT+TGG - Chr2:24855342-24855361 MsG0280008050.01.T01:intron 25.0%
! AGTGATCTTAATTGAAAAAG+AGG + Chr2:24856376-24856395 None:intergenic 25.0%
! ATTATAAGTATAAGTCGCTT+TGG + Chr2:24855341-24855360 None:intergenic 25.0%
! CTTATACTTATAATTTGGGA+CGG - Chr2:24855347-24855366 MsG0280008050.01.T01:intron 25.0%
! GCGACTTATACTTATAATTT+GGG - Chr2:24855343-24855362 MsG0280008050.01.T01:intron 25.0%
! TCAAAAGTTTATCATCATTG+GGG + Chr2:24854877-24854896 None:intergenic 25.0%
! TCCCAAATTATAAGTAAAGT+AGG - Chr2:24855250-24855269 MsG0280008050.01.T01:intron 25.0%
!! ACATATATAACCCTCTAAAA+AGG + Chr2:24854800-24854819 None:intergenic 25.0%
!! ATAAGCTAACATTTTTACCA+TGG + Chr2:24853614-24853633 None:intergenic 25.0%
!! GTTATTTGAACAACTCTTTT+TGG - Chr2:24853965-24853984 MsG0280008050.01.T01:CDS 25.0%
!! TCAAATATAGTGAGTTGAAA+GGG - Chr2:24853652-24853671 MsG0280008050.01.T01:CDS 25.0%
!! TGTATTGTTTTCTGAATTTC+AGG - Chr2:24855982-24856001 MsG0280008050.01.T01:intron 25.0%
!! TTATAAGTATAAGTCGCTTT+GGG + Chr2:24855340-24855359 None:intergenic 25.0%
!! TTTTATGTTTGGACTGTTAA+TGG - Chr2:24856223-24856242 MsG0280008050.01.T01:intron 25.0%
!!! CATTACCAAATCTTTTAGAA+GGG + Chr2:24855685-24855704 None:intergenic 25.0%
!!! TCACTCCTTTTTTTTTCTTT+TGG + Chr2:24853519-24853538 None:intergenic 25.0%
!!! TGACTTGTTGTAGTTATTTT+TGG + Chr2:24853563-24853582 None:intergenic 25.0%
AAATAGCACTTTCTGATTTC+TGG - Chr2:24856296-24856315 MsG0280008050.01.T01:intron 30.0%
AATAAGCTGAAAACAGCTTA+TGG - Chr2:24854992-24855011 MsG0280008050.01.T01:intron 30.0%
AGAGAAAAAGTAAAAACACG+GGG - Chr2:24854059-24854078 MsG0280008050.01.T01:CDS 30.0%
ATAACATGGTTGCTTCAAAT+AGG - Chr2:24853378-24853397 MsG0280008050.01.T01:CDS 30.0%
ATTGAAAGAGATGTTACCAA+TGG + Chr2:24854764-24854783 None:intergenic 30.0%
CTTTCAACTCACTATATTTG+AGG + Chr2:24853653-24853672 None:intergenic 30.0%
GAGAGAAAAAGTAAAAACAC+GGG - Chr2:24854058-24854077 MsG0280008050.01.T01:CDS 30.0%
GTATTGTTGTGTACATACAT+TGG - Chr2:24854915-24854934 MsG0280008050.01.T01:intron 30.0%
TAGAGGGTTATATATGTTTC+TGG - Chr2:24854803-24854822 MsG0280008050.01.T01:intron 30.0%
! AGTTTTACAAGTGTTTATGC+GGG - Chr2:24855051-24855070 MsG0280008050.01.T01:intron 30.0%
! ATATATGTTGGCCTCAAATA+TGG - Chr2:24853696-24853715 MsG0280008050.01.T01:CDS 30.0%
! ATTTTATACAACTTCTTCGG+AGG + Chr2:24854513-24854532 None:intergenic 30.0%
! CAGTTTTACAAGTGTTTATG+CGG - Chr2:24855050-24855069 MsG0280008050.01.T01:intron 30.0%
! CCATTACCAAATCTTTTAGA+AGG + Chr2:24855686-24855705 None:intergenic 30.0%
! CCTTCTAAAAGATTTGGTAA+TGG - Chr2:24855683-24855702 MsG0280008050.01.T01:intron 30.0%
! CTCAAATATAGTGAGTTGAA+AGG - Chr2:24853651-24853670 MsG0280008050.01.T01:CDS 30.0%
! GATAACCCTTCTAAAAGATT+TGG - Chr2:24855677-24855696 MsG0280008050.01.T01:intron 30.0%
! GGTAAAAATGTTAGCTTATG+AGG - Chr2:24853615-24853634 MsG0280008050.01.T01:CDS 30.0%
! TTTAATGGAGATTCTAGGAA+GGG - Chr2:24856189-24856208 MsG0280008050.01.T01:intron 30.0%
!! AATCTTTTAGAAGGGTTATC+AGG + Chr2:24855677-24855696 None:intergenic 30.0%
!! ACTGGATGGATTTTTATGTT+TGG - Chr2:24856212-24856231 MsG0280008050.01.T01:intron 30.0%
!! GCATTTCTGAAATACAATCA+TGG + Chr2:24854709-24854728 None:intergenic 30.0%
!! TCTAAAAGATTTGGTAATGG+TGG - Chr2:24855686-24855705 MsG0280008050.01.T01:intron 30.0%
!! TTTCTGAAATACAATCATGG+TGG + Chr2:24854706-24854725 None:intergenic 30.0%
!! TTTTCTGAATTTCAGGTCTA+CGG - Chr2:24855989-24856008 MsG0280008050.01.T01:intron 30.0%
!!! CTTGTTGTAGTTATTTTTGG+TGG + Chr2:24853560-24853579 None:intergenic 30.0%
AACCATGTTATCTCTACTTC+TGG + Chr2:24853369-24853388 None:intergenic 35.0%
ACGACATAATTCATGTCTAG+GGG + Chr2:24856407-24856426 None:intergenic 35.0%
AGTCAACAATGACTACAAAG+AGG - Chr2:24853578-24853597 MsG0280008050.01.T01:CDS 35.0%
ATCCTATCAATTGTACCATC+CGG + Chr2:24856049-24856068 None:intergenic 35.0%
ATGTTGGAGCAATATCAAAG+AGG - Chr2:24855919-24855938 MsG0280008050.01.T01:intron 35.0%
ATTACTCAAACACGCAACAA+GGG + Chr2:24855627-24855646 None:intergenic 35.0%
CACGACATAATTCATGTCTA+GGG + Chr2:24856408-24856427 None:intergenic 35.0%
CACGGAAGTAGCTATTATAA+GGG - Chr2:24855599-24855618 MsG0280008050.01.T01:intron 35.0%
CATGTTGAAATTCACTTCTG+TGG - Chr2:24855712-24855731 MsG0280008050.01.T01:intron 35.0%
CTTTCAACTCACCATATTTG+AGG + Chr2:24853710-24853729 None:intergenic 35.0%
GAAAACAGAAAACACGATGA+AGG - Chr2:24854581-24854600 MsG0280008050.01.T01:intron 35.0%
GAAAGATACAAACTGATGAC+TGG - Chr2:24855953-24855972 MsG0280008050.01.T01:intron 35.0%
GTGCAAAACAACAAGTAAAG+TGG + Chr2:24855174-24855193 None:intergenic 35.0%
TACCAGAAGTAGAGATAACA+TGG - Chr2:24853364-24853383 MsG0280008050.01.T01:CDS 35.0%
TATATCGTGATCCAGAGTAT+TGG - Chr2:24856263-24856282 MsG0280008050.01.T01:intron 35.0%
TATTACTCAAACACGCAACA+AGG + Chr2:24855628-24855647 None:intergenic 35.0%
TCACGACATAATTCATGTCT+AGG + Chr2:24856409-24856428 None:intergenic 35.0%
TGAGATAAGAGAGAAATCGA+TGG - Chr2:24854672-24854691 MsG0280008050.01.T01:intron 35.0%
TTAATGGCAGTAGTCTATTC+AGG - Chr2:24856239-24856258 MsG0280008050.01.T01:intron 35.0%
TTCATTACATCCCAATACTC+TGG + Chr2:24856277-24856296 None:intergenic 35.0%
TTTGATATTGCTCCAACATG+TGG + Chr2:24855918-24855937 None:intergenic 35.0%
! AAACGAACTTGCTTGGATTT+GGG - Chr2:24854543-24854562 MsG0280008050.01.T01:intron 35.0%
! AGCTTATGAGGTTCTTTTGT+TGG - Chr2:24853627-24853646 MsG0280008050.01.T01:CDS 35.0%
! ATATCGTGATCCAGAGTATT+GGG - Chr2:24856264-24856283 MsG0280008050.01.T01:intron 35.0%
! CATACTCACTCCTTTTTAGA+GGG - Chr2:24854787-24854806 MsG0280008050.01.T01:intron 35.0%
! GTTTAATGGAGATTCTAGGA+AGG - Chr2:24856188-24856207 MsG0280008050.01.T01:intron 35.0%
! TAAACGAACTTGCTTGGATT+TGG - Chr2:24854542-24854561 MsG0280008050.01.T01:intron 35.0%
! TACTTTCTCCTCTTTTACAG+TGG - Chr2:24855530-24855549 MsG0280008050.01.T01:intron 35.0%
! TCATACTCACTCCTTTTTAG+AGG - Chr2:24854786-24854805 MsG0280008050.01.T01:intron 35.0%
!! AAAAGATTTGGTAATGGTGG+TGG - Chr2:24855689-24855708 MsG0280008050.01.T01:intron 35.0%
!!! ACTCTTTTTGGGATAACTTG+TGG - Chr2:24853977-24853996 MsG0280008050.01.T01:CDS 35.0%
!!! CTCTTTTTGGGATAACTTGT+GGG - Chr2:24853978-24853997 MsG0280008050.01.T01:CDS 35.0%
!!! GTTGTAGTTATTTTTGGTGG+CGG + Chr2:24853557-24853576 None:intergenic 35.0%
!!! TTTTCTTTTGGCTTTTGCAC+CGG + Chr2:24853507-24853526 None:intergenic 35.0%
AGAATCGTGAATGTGCTATG+GGG - Chr2:24854631-24854650 MsG0280008050.01.T01:intron 40.0%
AGAGAATCGTGAATGTGCTA+TGG - Chr2:24854629-24854648 MsG0280008050.01.T01:intron 40.0%
CAAATAGGAGTGAAACTGCT+GGG - Chr2:24853393-24853412 MsG0280008050.01.T01:CDS 40.0%
CACTATATTCCTCAAGCTCA+GGG - Chr2:24856166-24856185 MsG0280008050.01.T01:intron 40.0%
CAGCTATGGTTAGAGAAGAT+TGG - Chr2:24855863-24855882 MsG0280008050.01.T01:intron 40.0%
CAGGGTTTAATGGAGATTCT+AGG - Chr2:24856184-24856203 MsG0280008050.01.T01:intron 40.0%
CATAATTCATGTCTAGGGGA+TGG + Chr2:24856403-24856422 None:intergenic 40.0%
CGATTTCTCTCTTATCTCAG+CGG + Chr2:24854671-24854690 None:intergenic 40.0%
CTGGCATGTTATACTCATGT+GGG - Chr2:24854822-24854841 MsG0280008050.01.T01:intron 40.0%
GAGAATCGTGAATGTGCTAT+GGG - Chr2:24854630-24854649 MsG0280008050.01.T01:intron 40.0%
GAGGTTAAGTGTCCGTATTT+CGG - Chr2:24856100-24856119 MsG0280008050.01.T01:intron 40.0%
GCACGGAAGTAGCTATTATA+AGG - Chr2:24855598-24855617 MsG0280008050.01.T01:intron 40.0%
GCTTGAGGAATATAGTGAAC+TGG + Chr2:24856163-24856182 None:intergenic 40.0%
GGATGGTACAATTGATAGGA+TGG - Chr2:24856048-24856067 MsG0280008050.01.T01:intron 40.0%
GTTACCAATGGAATGTTGAG+CGG + Chr2:24854752-24854771 None:intergenic 40.0%
GTTCAAAACCAGAAGACAGT+TGG - Chr2:24856011-24856030 MsG0280008050.01.T01:intron 40.0%
TCAAATAGGAGTGAAACTGC+TGG - Chr2:24853392-24853411 MsG0280008050.01.T01:CDS 40.0%
TCACTATATTCCTCAAGCTC+AGG - Chr2:24856165-24856184 MsG0280008050.01.T01:intron 40.0%
TCTGGCATGTTATACTCATG+TGG - Chr2:24854821-24854840 MsG0280008050.01.T01:intron 40.0%
TGAACTGGAATTCGATACCA+AGG + Chr2:24856148-24856167 None:intergenic 40.0%
TGGCATGTTATACTCATGTG+GGG - Chr2:24854823-24854842 MsG0280008050.01.T01:intron 40.0%
TGTGTGCAACATTTCAAGCA+CGG - Chr2:24855581-24855600 MsG0280008050.01.T01:intron 40.0%
TTAAGAACTGGCAGACACTT+AGG - Chr2:24855777-24855796 MsG0280008050.01.T01:intron 40.0%
! AGTTCTTAAACCAGTGTTGG+AGG + Chr2:24855766-24855785 None:intergenic 40.0%
! ATTTTTACCATGGTTCAGCG+AGG + Chr2:24853604-24853623 None:intergenic 40.0%
! CGATTTTGCCACTGTAAAAG+AGG + Chr2:24855541-24855560 None:intergenic 40.0%
! GTATATGATTTGCCTTCTCG+TGG - Chr2:24856070-24856089 MsG0280008050.01.T01:intron 40.0%
! TAGCACTTTCTGATTTCTGG+TGG - Chr2:24856299-24856318 MsG0280008050.01.T01:intron 40.0%
!! GGTGCTATAGAACCATTTTC+TGG - Chr2:24855884-24855903 MsG0280008050.01.T01:intron 40.0%
!! TCCAACACTGGTTTAAGAAC+TGG - Chr2:24855765-24855784 MsG0280008050.01.T01:intron 40.0%
AATCGTGAATGTGCTATGGG+GGG - Chr2:24854633-24854652 MsG0280008050.01.T01:intron 45.0%
ACATGAGTAAGGCTTTGCCT+TGG - Chr2:24856128-24856147 MsG0280008050.01.T01:intron 45.0%
ATTAGTCGTCCACCTTTGGT+GGG - Chr2:24853474-24853493 MsG0280008050.01.T01:CDS 45.0%
CACCGGATGGTACAATTGAT+AGG - Chr2:24856044-24856063 MsG0280008050.01.T01:intron 45.0%
CACTTCTGTGGAAACACACT+CGG - Chr2:24855724-24855743 MsG0280008050.01.T01:intron 45.0%
CTCATGTCTCCACCGAAATA+CGG + Chr2:24856115-24856134 None:intergenic 45.0%
CTGCTATACAACTCTCTTGC+CGG + Chr2:24856337-24856356 None:intergenic 45.0%
GAATCGTGAATGTGCTATGG+GGG - Chr2:24854632-24854651 MsG0280008050.01.T01:intron 45.0%
GAGAGAAATCGATGGCTTCA+TGG - Chr2:24854680-24854699 MsG0280008050.01.T01:intron 45.0%
GCAAGAGAGTTGTATAGCAG+TGG - Chr2:24856337-24856356 MsG0280008050.01.T01:intron 45.0%
GCCAGTTCTTAAACCAGTGT+TGG + Chr2:24855769-24855788 None:intergenic 45.0%
GTAAGCGAAGATCTCAGCTA+TGG - Chr2:24855849-24855868 MsG0280008050.01.T01:intron 45.0%
GTGGCAAGGTTTCCAGAAAA+TGG + Chr2:24855899-24855918 None:intergenic 45.0%
GTTAAGTGTCCGTATTTCGG+TGG - Chr2:24856103-24856122 MsG0280008050.01.T01:intron 45.0%
TAGAAGGGTTATCAGGAGCA+TGG + Chr2:24855670-24855689 None:intergenic 45.0%
TATTGCTCCAACATGTGGCA+AGG + Chr2:24855913-24855932 None:intergenic 45.0%
TCCAAGCAAGTTCGTTTACG+TGG + Chr2:24854540-24854559 None:intergenic 45.0%
TCCATTAAACCCTGAGCTTG+AGG + Chr2:24856178-24856197 None:intergenic 45.0%
TCCTCAAGCTCAGGGTTTAA+TGG - Chr2:24856174-24856193 MsG0280008050.01.T01:intron 45.0%
TTTCGGTGGAGACATGAGTA+AGG - Chr2:24856117-24856136 MsG0280008050.01.T01:intron 45.0%
! AACGGCTAGTACTTTTGCTG+CGG - Chr2:24855811-24855830 MsG0280008050.01.T01:intron 45.0%
! ACCACGTAAACGAACTTGCT+TGG - Chr2:24854536-24854555 MsG0280008050.01.T01:intron 45.0%
! TTAAACCAGTGTTGGAGGCA+TGG + Chr2:24855761-24855780 None:intergenic 45.0%
!! TTGGTGGCGGTTATAGAGTA+CGG + Chr2:24853544-24853563 None:intergenic 45.0%
AACTGCTGGGTTCGATTCCT+AGG - Chr2:24853406-24853425 MsG0280008050.01.T01:CDS 50.0%
CTAATCCGACTCATGGGTAG+AGG + Chr2:24853459-24853478 None:intergenic 50.0%
CTGGAAACCTTGCCACATGT+TGG - Chr2:24855903-24855922 MsG0280008050.01.T01:intron 50.0%
CTTGCTTGGATTTGGGAGAG+AGG - Chr2:24854550-24854569 MsG0280008050.01.T01:intron 50.0%
GGACGACTAATCCGACTCAT+GGG + Chr2:24853465-24853484 None:intergenic 50.0%
TCGCTTACGCCAAAACCCAA+TGG + Chr2:24855837-24855856 None:intergenic 50.0%
TGGACGACTAATCCGACTCA+TGG + Chr2:24853466-24853485 None:intergenic 50.0%
TTCCTAGGAGGAACAACGCT+TGG - Chr2:24853421-24853440 MsG0280008050.01.T01:CDS 50.0%
TTTGCCTTCTCGTGGTGTAC+TGG - Chr2:24856078-24856097 MsG0280008050.01.T01:intron 50.0%
! AGTACTTTTGCTGCGGCCAT+TGG - Chr2:24855818-24855837 MsG0280008050.01.T01:intron 50.0%
! GATTAGTCGTCCACCTTTGG+TGG - Chr2:24853473-24853492 MsG0280008050.01.T01:CDS 50.0%
! GATTCTAGGAAGGGACTGGA+TGG - Chr2:24856198-24856217 MsG0280008050.01.T01:intron 50.0%
! GTACTTTTGCTGCGGCCATT+GGG - Chr2:24855819-24855838 MsG0280008050.01.T01:intron 50.0%
! TGGAGATTCTAGGAAGGGAC+TGG - Chr2:24856194-24856213 MsG0280008050.01.T01:intron 50.0%
!! TCGGATTAGTCGTCCACCTT+TGG - Chr2:24853470-24853489 MsG0280008050.01.T01:CDS 50.0%
AAAGAGGCCTCGCTGAACCA+TGG - Chr2:24853594-24853613 MsG0280008050.01.T01:CDS 55.0%
AAGCACCATGCCTCCAACAC+TGG - Chr2:24855753-24855772 MsG0280008050.01.T01:intron 55.0%
ACCTCCAGTACACCACGAGA+AGG + Chr2:24856085-24856104 None:intergenic 55.0%
GCATGCCTCTACCCATGAGT+CGG - Chr2:24853451-24853470 MsG0280008050.01.T01:CDS 55.0%
TGCGCCGCTCAACATTCCAT+TGG - Chr2:24854745-24854764 MsG0280008050.01.T01:intron 55.0%
TGCTGGGTTCGATTCCTAGG+AGG - Chr2:24853409-24853428 MsG0280008050.01.T01:CDS 55.0%
! ACCTTTGGTGGGTCGGAAAC+CGG - Chr2:24853485-24853504 MsG0280008050.01.T01:CDS 55.0%
! CTGGTGGAAGCATGTTCAGC+CGG - Chr2:24856315-24856334 MsG0280008050.01.T01:intron 55.0%
! TTGCTGCGGCCATTGGGTTT+TGG - Chr2:24855825-24855844 MsG0280008050.01.T01:intron 55.0%
GCAGCGAGCCAACTGTCTTC+TGG + Chr2:24856022-24856041 None:intergenic 60.0%
GCCTTCTCGTGGTGTACTGG+AGG - Chr2:24856081-24856100 MsG0280008050.01.T01:intron 60.0%
GGTTTCCGACCCACCAAAGG+TGG + Chr2:24853486-24853505 None:intergenic 60.0%
! ACCGGTTTCCGACCCACCAA+AGG + Chr2:24853489-24853508 None:intergenic 60.0%
! ATGGGTAGAGGCATGCGCAC+TGG + Chr2:24853447-24853466 None:intergenic 60.0%
! GGCCAAGCGTTGTTCCTCCT+AGG + Chr2:24853426-24853445 None:intergenic 60.0%
! GTCGTCCACCTTTGGTGGGT+CGG - Chr2:24853478-24853497 MsG0280008050.01.T01:CDS 60.0%
!! CAGTTGGCTCGCTGCTTCAC+CGG - Chr2:24856027-24856046 MsG0280008050.01.T01:intron 60.0%
TGGCTCGCTGCTTCACCGGA+TGG - Chr2:24856031-24856050 MsG0280008050.01.T01:intron 65.0%
Chromosome Type Strat End Strand Name
Chr2 gene 24853344 24856456 24853344 ID=MsG0280008050.01;Name=MsG0280008050.01
Chr2 mRNA 24853344 24856456 24853344 ID=MsG0280008050.01.T01;Parent=MsG0280008050.01;Name=MsG0280008050.01.T01;_AED=0.49;_eAED=0.53;_QI=0|0|0|1|1|1|2|0|325
Chr2 exon 24856386 24856456 24856386 ID=MsG0280008050.01.T01:exon:34855;Parent=MsG0280008050.01.T01
Chr2 exon 24853344 24854250 24853344 ID=MsG0280008050.01.T01:exon:34854;Parent=MsG0280008050.01.T01
Chr2 CDS 24856386 24856456 24856386 ID=MsG0280008050.01.T01:cds;Parent=MsG0280008050.01.T01
Chr2 CDS 24853344 24854250 24853344 ID=MsG0280008050.01.T01:cds;Parent=MsG0280008050.01.T01
Gene Sequence

>MsG0280008050.01.T01

ATGGCTAACAAATACTACAATACCAGAAGTAGAGATAACATGGTTGCTTCAAATAGGAGTGAAACTGCTGGTGGCAAAATCGATTCTTCATTATCTGTTGCTTGTGTGCAACATTTCAAGCACGGAAGTAGCTATTATAAGGGTGCCCTTGTTGCGTGTTTGAGTAATAAGAGAATTTTTTACAGTAGCCATGCTCCTGATAACCCTTCTAAAAGATTTGGTAATGGTGGTGGCATGTTGAAATTCACTTCTGTGGAAACACACTCGGTTCTTGAAGCACCATGCCTCCAACACTGGTTTAAGAACTGGCAGACACTTAGGAAACAAAAGTTAACGGCTAGTACTTTTGCTGCGGCCATTGGGTTTTGGCGTAAGCGAAGATCTCAGCTATGGTTAGAGAAGATTGGTGCTATAGAACCATTTTCTGGAAACCTTGCCACATGTTGGAGCAATATCAAAGAGGAAGAAGCACTTGAAAGATACAAACTGATGACTGGAAATACTGTATTGTTTTCTGAATTTCAGGTCTACGGTTCAAAACCAGAAGACAGTTGGCTCGCTGCTTCACCGGATGGTACAATTGATAGGATGGTATATGATTTGCCTTCTCGTGGTGTACTGGAGGTTAAGTGTCCGTATTTCGGTGGAGACATGAGTAAGGCTTTGCCTTGGTATCGAATTCCAGTTCACTATATTCCTCAAGCTCAGGGTTTAATGGAGATTCTAGGAAGGGACTGGATGGATTTTTATGTTTGGACTGTTAATGGCAGTAGTCTATTCAGGATATATCGTGATCCAGAGTATTGGGATGTAATGAAAATAGCACTTTCTGATTTCTGGTGGAAGCATGTTCAGCCGGCAAGAGAGTTGTATAGCAGTGGTGTTATAAAAGATCCTCTTTTTCAATTAAGATCACTAACACCATCCCCTAGACATGAATTATGTCGTGATATAGTTTATAAAAGTAAATATTGTTGA
Protein sequence

>MsG0280008050.01.T01

MANKYYNTRSRDNMVASNRSETAGGKIDSSLSVACVQHFKHGSSYYKGALVACLSNKRIFYSSHAPDNPSKRFGNGGGMLKFTSVETHSVLEAPCLQHWFKNWQTLRKQKLTASTFAAAIGFWRKRRSQLWLEKIGAIEPFSGNLATCWSNIKEEEALERYKLMTGNTVLFSEFQVYGSKPEDSWLAASPDGTIDRMVYDLPSRGVLEVKCPYFGGDMSKALPWYRIPVHYIPQAQGLMEILGRDWMDFYVWTVNGSSLFRIYRDPEYWDVMKIALSDFWWKHVQPARELYSSGVIKDPLFQLRSLTPSPRHELCRDIVYKSKYC*