AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar ZhongmuNo.1 / MsG0780041440.01


Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MsG0780041440.01.T01 MTR_7g111090 88.889 27 3 0 42 68 1 27 9.11e-11 52.4
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score

Find 5 sgRNAs with CRISPR-Local

Find 22 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
AAAATGGCAGAACTGAGTTT+GGG 0.446438 7:-90925256 MsG0780041440.01.T01:CDS
TTGACATTGTTGATGAGGAT+TGG 0.447381 7:-90925225 MsG0780041440.01.T01:CDS
TCTAATTGACATTGTTGATG+AGG 0.512652 7:-90925230 MsG0780041440.01.T01:CDS
AGAGACATTCTTCCTCAAGA+TGG 0.570997 7:-90925199 MsG0780041440.01.T01:CDS
ATTGTTCTCAATCTGAGTGA+AGG 0.633691 7:-90925475 MsG0780041440.01.T01:CDS

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!! AAAAAAAAAATACCATCTTG+AGG + Chr7:90925504-90925523 None:intergenic 20.0%
! AATGAAGAGAAAGATGAATT+TGG + Chr7:90925314-90925333 None:intergenic 25.0%
!! AAACGACAAGAATTAGATTT+TGG + Chr7:90925384-90925403 None:intergenic 25.0%
!! AACGACAAGAATTAGATTTT+GGG + Chr7:90925383-90925402 None:intergenic 25.0%
!!! ATTTTGTGTTATTAACAGTG+TGG - Chr7:90925405-90925424 MsG0780041440.01.T01:intron 25.0%
TCTAATTGACATTGTTGATG+AGG - Chr7:90925458-90925477 MsG0780041440.01.T01:CDS 30.0%
TTAACAGTGTGGAGAAAAAA+TGG - Chr7:90925416-90925435 MsG0780041440.01.T01:intron 30.0%
! ACGACAAGAATTAGATTTTG+GGG + Chr7:90925382-90925401 None:intergenic 30.0%
! GGTTAGGGATAATTTGAAAT+TGG + Chr7:90925354-90925373 None:intergenic 30.0%
AAAAATGGCAGAACTGAGTT+TGG - Chr7:90925431-90925450 MsG0780041440.01.T01:CDS 35.0%
AAAATGGCAGAACTGAGTTT+GGG - Chr7:90925432-90925451 MsG0780041440.01.T01:CDS 35.0%
ATTGTTCTCAATCTGAGTGA+AGG - Chr7:90925213-90925232 MsG0780041440.01.T01:CDS 35.0%
GAATTTGGAGAAGAGAGATA+AGG + Chr7:90925299-90925318 None:intergenic 35.0%
! CGACAAGAATTAGATTTTGG+GGG + Chr7:90925381-90925400 None:intergenic 35.0%
! TTGACATTGTTGATGAGGAT+TGG - Chr7:90925463-90925482 MsG0780041440.01.T01:CDS 35.0%
!! AGAATTAGATTTTGGGGGTT+AGG + Chr7:90925376-90925395 None:intergenic 35.0%
!! GAATTAGATTTTGGGGGTTA+GGG + Chr7:90925375-90925394 None:intergenic 35.0%
AGAGACATTCTTCCTCAAGA+TGG - Chr7:90925489-90925508 MsG0780041440.01.T01:CDS 40.0%
!! AGATTTTGGGGGTTAGGGTT+AGG + Chr7:90925370-90925389 None:intergenic 45.0%
!! CTGAGTGAAGGCAGTTTTAG+TGG - Chr7:90925225-90925244 MsG0780041440.01.T01:CDS 45.0%
!! GATTTTGGGGGTTAGGGTTA+GGG + Chr7:90925369-90925388 None:intergenic 45.0%
TCGCTGAGTCTCAGTCAGTC+AGG - Chr7:90925271-90925290 MsG0780041440.01.T01:intron 55.0%
Chromosome Type Strat End Strand Name
Chr7 gene 90925177 90925533 90925177 ID=MsG0780041440.01;Name=MsG0780041440.01
Chr7 mRNA 90925177 90925533 90925177 ID=MsG0780041440.01.T01;Parent=MsG0780041440.01;Name=MsG0780041440.01.T01;_AED=0.49;_eAED=0.76;_QI=0|0|0|1|1|1|2|0|73
Chr7 exon 90925423 90925533 90925423 ID=MsG0780041440.01.T01:exon:3900;Parent=MsG0780041440.01.T01
Chr7 exon 90925177 90925287 90925177 ID=MsG0780041440.01.T01:exon:3899;Parent=MsG0780041440.01.T01
Chr7 CDS 90925423 90925533 90925423 ID=MsG0780041440.01.T01:cds;Parent=MsG0780041440.01.T01
Chr7 CDS 90925177 90925287 90925177 ID=MsG0780041440.01.T01:cds;Parent=MsG0780041440.01.T01
Gene Sequence

>MsG0780041440.01.T01

ATGGTAGCGATTAAAGTTAATAATTTGAATACACACATTGTTCTCAATCTGAGTGAAGGCAGTTTTAGTGGCAATTTGAAATTGATTGATCGATTCGCTGAGTCTCAGTCATGTGGAGAAAAAATGGCAGAACTGAGTTTGGGAATTCTAATTGACATTGTTGATGAGGATTGGATGAGAGACATTCTTCCTCAAGATGGTATTTTTTTTTTTCTTCTTTAA

Protein sequence

>MsG0780041440.01.T01

MVAIKVNNLNTHIVLNLSEGSFSGNLKLIDRFAESQSCGEKMAELSLGILIDIVDEDWMRDILPQDGIFFFLL*