AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar ZhongmuNo.1 / MsG0280008109.01

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MsG0280008109.01.T01 MTR_2g035790 81.989 533 41 3 4 526 1 488 0.0 823
MsG0280008109.01.T01 MTR_4g094732 60.396 505 150 4 25 526 23 480 0.0 579
MsG0280008109.01.T01 MTR_2g035760 46.943 458 180 9 62 514 82 481 6.34e-125 375
MsG0280008109.01.T01 MTR_6g015785 47.619 462 174 9 69 526 93 490 8.23e-118 357
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MsG0280008109.01.T01 AT4G20040 55.957 470 157 7 58 526 63 483 2.45e-178 511
MsG0280008109.01.T01 AT4G20050 43.691 531 225 10 6 526 13 479 1.58e-130 389
MsG0280008109.01.T01 AT4G20050 43.691 531 225 10 6 526 13 479 1.58e-130 389
MsG0280008109.01.T01 AT4G20050 43.691 531 225 10 6 526 13 479 1.58e-130 389
MsG0280008109.01.T01 AT4G20050 43.691 531 225 10 6 526 13 479 1.58e-130 389
MsG0280008109.01.T01 AT4G20050 43.691 531 225 10 6 526 38 504 1.91e-130 389
MsG0280008109.01.T01 AT4G20050 46.742 445 168 9 87 526 1 381 2.00e-117 352
MsG0280008109.01.T01 AT4G20050 46.742 445 168 9 87 526 1 381 2.00e-117 352

Find 92 sgRNAs with CRISPR-Local

Find 188 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
GCATTTGATATTGATAATTT+AGG 0.107857 2:+25576503 MsG0280008109.01.T01:CDS
ACAATTGGAACATTATTATT+AGG 0.129299 2:-25578715 None:intergenic
GGATCTGCACCATACTTAAT+TGG 0.153680 2:-25576431 None:intergenic
TCAATTAAAGGGAAAGTTTA+TGG 0.171584 2:+25578658 MsG0280008109.01.T01:CDS
AGCATTTCTCTTGTCACTTT+TGG 0.236867 2:+25575999 MsG0280008109.01.T01:CDS
AAAATGTTCCAGAAGCTCTT+AGG 0.237979 2:-25578013 None:intergenic
GTGTTTCCTAGTACGCTTCT+AGG 0.250409 2:-25577238 None:intergenic
CATTTGATATTGATAATTTA+GGG 0.251344 2:+25576504 MsG0280008109.01.T01:CDS
GTTCCAATTGTAAAATTAGA+TGG 0.268649 2:+25578727 MsG0280008109.01.T01:CDS
CAATTGGAACATTATTATTA+GGG 0.285274 2:-25578714 None:intergenic
ATTGTTAACAATATGTTTAG+TGG 0.301676 2:+25578688 MsG0280008109.01.T01:CDS
TATAACAAAGCAACATGTTT+TGG 0.304133 2:+25578484 MsG0280008109.01.T01:CDS
TGAAAATTACTAATCCTATT+AGG 0.312117 2:-25578889 None:intergenic
GTTCAAGTTCATGTTACTAA+TGG 0.312426 2:+25578601 MsG0280008109.01.T01:CDS
GGAGGAACCCTAAGAGCTTC+TGG 0.336228 2:+25578005 MsG0280008109.01.T01:CDS
GCCATTGGAATTGTTGTTAG+AGG 0.339653 2:+25578430 MsG0280008109.01.T01:CDS
TGGCTGGTAATGGCACAAAA+TGG 0.363806 2:+25578836 MsG0280008109.01.T01:CDS
TAATTCCTTCTGTGTTGAAA+TGG 0.369391 2:-25578252 None:intergenic
TACTTGATATGAAGGTTTCA+TGG 0.388165 2:-25578288 None:intergenic
AGGGTTCCTCCGTTTACCTT+TGG 0.390394 2:-25577993 None:intergenic
CCTCCAAGGTCTCTAATTCC+TGG 0.397675 2:-25576539 None:intergenic
ACTGAGTTTGCAACAAACTT+TGG 0.400846 2:-25578937 None:intergenic
ATGGAGTATTAAGAAATTGC+AGG 0.403829 2:-25577264 None:intergenic
ATCACATTTCCTTCATCTGC+TGG 0.408504 2:+25576608 MsG0280008109.01.T01:CDS
AGCTTCTACAGCTTTCATAA+TGG 0.424025 2:-25576480 None:intergenic
TTGTTCAAGTAATCTGTTGT+TGG 0.430783 2:-25578107 None:intergenic
AAGAAAACTACTTGATATGA+AGG 0.434177 2:-25578296 None:intergenic
AAATGTTCCAGAAGCTCTTA+GGG 0.436109 2:-25578012 None:intergenic
GATCTGCACCATACTTAATT+GGG 0.441924 2:-25576430 None:intergenic
CTGCTTTCATTCACTCCAGT+TGG 0.444510 2:-25576452 None:intergenic
TTAGTAACATGAACTTGAAC+AGG 0.445078 2:-25578598 None:intergenic
CATCAATGACCAAAGGTAAA+CGG 0.458709 2:+25577984 MsG0280008109.01.T01:CDS
TCAACAAGGTGTCTTTCAGT+TGG 0.459768 2:-25578037 None:intergenic
TTCCAATTGTAAAATTAGAT+GGG 0.461356 2:+25578728 MsG0280008109.01.T01:CDS
TTGAGTGAGACTTACATTGA+TGG 0.463481 2:-25577283 None:intergenic
AGATTACTTGAACAAACAAT+AGG 0.463517 2:+25578115 MsG0280008109.01.T01:CDS
GTATTCATTTCATACATTAG+AGG 0.463619 2:+25578912 MsG0280008109.01.T01:CDS
ATTGATATGTAACAGTTAAG+TGG 0.465928 2:-25577940 None:intergenic
CTTGGACAACACTCAACAGT+TGG 0.484090 2:+25578316 MsG0280008109.01.T01:CDS
AAGGAAACAACACCATGAAC+TGG 0.487041 2:-25579003 None:intergenic
CCTGTACCTAGAAGCGTACT+AGG 0.487345 2:+25577232 MsG0280008109.01.T01:CDS
TATTCATTTCATACATTAGA+GGG 0.494492 2:+25578913 MsG0280008109.01.T01:CDS
TTACCAGGAATTAGAGACCT+TGG 0.494991 2:+25576536 MsG0280008109.01.T01:CDS
CCTAGTACGCTTCTAGGTAC+AGG 0.498372 2:-25577232 None:intergenic
GGCTGGTAATGGCACAAAAT+GGG 0.500320 2:+25578837 MsG0280008109.01.T01:CDS
TTCATCTGCTGGCAATCTTG+TGG 0.500388 2:+25576619 MsG0280008109.01.T01:CDS
GATTCGAGCTACCGAGGTGG+AGG 0.505643 2:+25578178 MsG0280008109.01.T01:CDS
TTCCTCCATTTCAACACAGA+AGG 0.508555 2:+25578247 MsG0280008109.01.T01:CDS
AAGTATGGTGCAGATCCAAC+TGG 0.512830 2:+25576437 MsG0280008109.01.T01:CDS
AACTCATTGTTCAACAAAGA+AGG 0.513850 2:-25579022 None:intergenic
TTAGGGCTTGAATTATTACC+AGG 0.514044 2:+25576521 MsG0280008109.01.T01:CDS
AAGTTGAATGTGGCTGGTAA+TGG 0.526491 2:+25578826 MsG0280008109.01.T01:CDS
GTGGTGTACCCAATTAAGTA+TGG 0.526549 2:+25576422 MsG0280008109.01.T01:CDS
ACAAGATTGCCAGCAGATGA+AGG 0.526613 2:-25576617 None:intergenic
GAAAGTGAGAAACCAGTTCA+TGG 0.531426 2:+25578991 MsG0280008109.01.T01:CDS
AAGAATTGATAACTGTTACA+TGG 0.534076 2:+25578549 MsG0280008109.01.T01:CDS
GATTGGAAAATGAAGAAGAG+TGG 0.536042 2:+25576395 MsG0280008109.01.T01:intron
ATTGATATGAACAATGTGAT+TGG 0.543536 2:+25578778 MsG0280008109.01.T01:CDS
TGGTAATGGCACAAAATGGG+TGG 0.549729 2:+25578840 MsG0280008109.01.T01:CDS
TTCCTTCTGTGTTGAAATGG+AGG 0.550409 2:-25578249 None:intergenic
TCAACAGTTGGCGGAGACAA+AGG 0.563052 2:+25578328 MsG0280008109.01.T01:CDS
TATGAACAATGTGATTGGTA+TGG 0.563112 2:+25578783 MsG0280008109.01.T01:CDS
GGATTATAATTCTATAGTCA+TGG 0.563335 2:+25578570 MsG0280008109.01.T01:CDS
TCCTCTAACAACAATTCCAA+TGG 0.565805 2:-25578431 None:intergenic
TCCAATTGTAAAATTAGATG+GGG 0.567825 2:+25578729 MsG0280008109.01.T01:CDS
GGACAACACTCAACAGTTGG+CGG 0.569761 2:+25578319 MsG0280008109.01.T01:CDS
AATGAAGAAGAGTGGAAGAG+TGG 0.571785 2:+25576403 MsG0280008109.01.T01:intron
ATTTAGTAACATTGATCAAG+TGG 0.575859 2:+25578753 MsG0280008109.01.T01:CDS
CCAGGAATTAGAGACCTTGG+AGG 0.587401 2:+25576539 MsG0280008109.01.T01:CDS
GGACAAGCTAACATGATCAC+AGG 0.590351 2:+25578451 MsG0280008109.01.T01:CDS
TTTACATGCACACAAATGAA+GGG 0.591535 2:-25575910 None:intergenic
GCAGATGAAGGAAATGTGAT+GGG 0.592160 2:-25576605 None:intergenic
GTTGGGAAGTTGAATGTGGC+TGG 0.594395 2:+25578820 MsG0280008109.01.T01:CDS
TGCTGAGAAAATTGCGACAT+CGG 0.594998 2:-25578407 None:intergenic
CTCTTCGATTCGAGCTACCG+AGG 0.598534 2:+25578172 MsG0280008109.01.T01:CDS
CCAATTGTAAAATTAGATGG+GGG 0.605323 2:+25578730 MsG0280008109.01.T01:CDS
AGCAGATGAAGGAAATGTGA+TGG 0.606062 2:-25576606 None:intergenic
AGTGATGACAATTTATGTAG+AGG 0.607887 2:-25575885 None:intergenic
AATCAATAATAACACCTCCA+AGG 0.611738 2:-25576553 None:intergenic
TACGTGTTCATTGAAGCAAG+TGG 0.612140 2:-25576043 None:intergenic
TTACATGCACACAAATGAAG+GGG 0.628033 2:-25575909 None:intergenic
GTGTGAAGATGGCTGAGACA+TGG 0.634929 2:-25576115 None:intergenic
GAAGGAATTAAAGTTCAAAG+TGG 0.637402 2:+25578265 MsG0280008109.01.T01:CDS
CTCGGTAGCTCGAATCGAAG+AGG 0.637806 2:-25578171 None:intergenic
GGTAATGGCACAAAATGGGT+GGG 0.641219 2:+25578841 MsG0280008109.01.T01:CDS
TTCGATTCGAGCTACCGAGG+TGG 0.641349 2:+25578175 MsG0280008109.01.T01:CDS
TTGATGCACTTAATTCAACA+AGG 0.647153 2:-25578051 None:intergenic
AGCCACATTCAACTTCCCAA+CGG 0.656399 2:-25578818 None:intergenic
AACCGTTGGGAAGTTGAATG+TGG 0.673926 2:+25578816 MsG0280008109.01.T01:CDS
TACATGCACACAAATGAAGG+GGG 0.676960 2:-25575908 None:intergenic
CAATGAATATTCCTCCACCT+CGG 0.699290 2:-25578189 None:intergenic
CAATGACCAAAGGTAAACGG+AGG 0.722922 2:+25577987 MsG0280008109.01.T01:CDS

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!! CAAAAAAAAAAAACAAAAAA+AGG + Chr2:25577563-25577582 MsG0280008109.01.T01:intron 10.0%
!! CATCAAAATTTATTAAATAT+AGG + Chr2:25576788-25576807 MsG0280008109.01.T01:intron 10.0%
!!! ATGAGATTTTTTAAAAATTA+AGG + Chr2:25577480-25577499 MsG0280008109.01.T01:intron 10.0%
!!! TTGATTTGATTTTTTTTTAT+TGG + Chr2:25577159-25577178 MsG0280008109.01.T01:intron 10.0%
!!! TTTTAATAATATTTGTTAAG+AGG + Chr2:25577067-25577086 MsG0280008109.01.T01:intron 10.0%
!! AAAAATTAAAACAAAGAAGA+GGG + Chr2:25576892-25576911 MsG0280008109.01.T01:intron 15.0%
!! CTAATTTATGTAACAAATTA+AGG - Chr2:25576740-25576759 None:intergenic 15.0%
!! TAAAAATTAAAACAAAGAAG+AGG + Chr2:25576891-25576910 MsG0280008109.01.T01:intron 15.0%
!! TTAAATAACATTATTTGGAA+TGG - Chr2:25577038-25577057 None:intergenic 15.0%
!!! CATTTGATATTGATAATTTA+GGG + Chr2:25576504-25576523 MsG0280008109.01.T01:CDS 15.0%
!!! CATTTTATACAATGAAAAAA+AGG - Chr2:25576342-25576361 None:intergenic 15.0%
!!! TAAATAACATTATTTGGAAT+GGG - Chr2:25577037-25577056 None:intergenic 15.0%
!!! TTTTGTTTTTTTTTTTTGTC+TGG - Chr2:25577561-25577580 None:intergenic 15.0%
!! AAAAATTGTCAAGCAAAAAA+TGG + Chr2:25576086-25576105 MsG0280008109.01.T01:CDS 20.0%
!! AAACAAAAAAAGGAAATTTG+TGG + Chr2:25577573-25577592 MsG0280008109.01.T01:intron 20.0%
!! AATCATAAAAAAAAACTCCT+AGG - Chr2:25576190-25576209 None:intergenic 20.0%
!! ACAATTGGAACATTATTATT+AGG - Chr2:25578718-25578737 None:intergenic 20.0%
!! ATTGTTAACAATATGTTTAG+TGG + Chr2:25578688-25578707 MsG0280008109.01.T01:CDS 20.0%
!! CAATTGGAACATTATTATTA+GGG - Chr2:25578717-25578736 None:intergenic 20.0%
!! CATAACATAACAATATTTGA+GGG + Chr2:25577863-25577882 MsG0280008109.01.T01:intron 20.0%
!! TATTCATTTCATACATTAGA+GGG + Chr2:25578913-25578932 MsG0280008109.01.T01:CDS 20.0%
!! TCATAACATAACAATATTTG+AGG + Chr2:25577862-25577881 MsG0280008109.01.T01:intron 20.0%
!! TGAAAATTACTAATCCTATT+AGG - Chr2:25578892-25578911 None:intergenic 20.0%
!! TGCATAAATTGAATTAAGAA+AGG - Chr2:25576682-25576701 None:intergenic 20.0%
!! TTCCAATTGTAAAATTAGAT+GGG + Chr2:25578728-25578747 MsG0280008109.01.T01:CDS 20.0%
!!! AAATAACATTATTTGGAATG+GGG - Chr2:25577036-25577055 None:intergenic 20.0%
!!! ATTGTTTTGAAGTCAATTAA+AGG + Chr2:25578646-25578665 MsG0280008109.01.T01:CDS 20.0%
!!! GCATTTGATATTGATAATTT+AGG + Chr2:25576503-25576522 MsG0280008109.01.T01:CDS 20.0%
!!! GTTATGTATGATTTTGATTT+TGG - Chr2:25575937-25575956 None:intergenic 20.0%
!!! TTACTAATGGTTTTTTCTTA+GGG + Chr2:25578614-25578633 MsG0280008109.01.T01:CDS 20.0%
!!! TTGTTTTGAAGTCAATTAAA+GGG + Chr2:25578647-25578666 MsG0280008109.01.T01:CDS 20.0%
!!! TTTTTATTTTCCTTTTTCCT+AGG + Chr2:25576170-25576189 MsG0280008109.01.T01:intron 20.0%
! AAAAAAAACTCCTAGGAAAA+AGG - Chr2:25576183-25576202 None:intergenic 25.0%
! AAGAAAACTACTTGATATGA+AGG - Chr2:25578299-25578318 None:intergenic 25.0%
! ACGATTCAATAGAACAAAAA+AGG + Chr2:25577435-25577454 MsG0280008109.01.T01:intron 25.0%
! AGATTACTTGAACAAACAAT+AGG + Chr2:25578115-25578134 MsG0280008109.01.T01:CDS 25.0%
! ATTGATATGAACAATGTGAT+TGG + Chr2:25578778-25578797 MsG0280008109.01.T01:CDS 25.0%
! ATTGATATGTAACAGTTAAG+TGG - Chr2:25577943-25577962 None:intergenic 25.0%
! ATTTAGTAACATTGATCAAG+TGG + Chr2:25578753-25578772 MsG0280008109.01.T01:CDS 25.0%
! GAGGAATAATAAGTTTGTAT+TGG + Chr2:25577720-25577739 MsG0280008109.01.T01:intron 25.0%
! GGATTATAATTCTATAGTCA+TGG + Chr2:25578570-25578589 MsG0280008109.01.T01:CDS 25.0%
! GTATTCATTTCATACATTAG+AGG + Chr2:25578912-25578931 MsG0280008109.01.T01:CDS 25.0%
! GTTCCAATTGTAAAATTAGA+TGG + Chr2:25578727-25578746 MsG0280008109.01.T01:CDS 25.0%
! TCAATTAAAGGGAAAGTTTA+TGG + Chr2:25578658-25578677 MsG0280008109.01.T01:CDS 25.0%
! TCCAATTGTAAAATTAGATG+GGG + Chr2:25578729-25578748 MsG0280008109.01.T01:CDS 25.0%
! TGAATTAAGAAAGGAGAAAA+AGG - Chr2:25576673-25576692 None:intergenic 25.0%
!! AAAAAAGCGAAAAAGTACAA+GGG + Chr2:25576837-25576856 MsG0280008109.01.T01:intron 25.0%
!! AAGAATTGATAACTGTTACA+TGG + Chr2:25578549-25578568 MsG0280008109.01.T01:CDS 25.0%
!! AGAAGCATATTAAGATGTTA+AGG - Chr2:25577827-25577846 None:intergenic 25.0%
!! ATCACACCATGTATATATTT+TGG + Chr2:25577695-25577714 MsG0280008109.01.T01:intron 25.0%
!! CATTTTTCTAACATTGTTTG+AGG + Chr2:25576267-25576286 MsG0280008109.01.T01:intron 25.0%
!! TATAACAAAGCAACATGTTT+TGG + Chr2:25578484-25578503 MsG0280008109.01.T01:CDS 25.0%
!! TATTCAATGCAAGAACATTT+TGG - Chr2:25577187-25577206 None:intergenic 25.0%
!! TTCATATCAAGTAGTTTTCT+TGG + Chr2:25578298-25578317 MsG0280008109.01.T01:CDS 25.0%
!!! GGTGTTATTATTGATTTTCA+AGG + Chr2:25576560-25576579 MsG0280008109.01.T01:CDS 25.0%
!!! GTTACTAATGGTTTTTTCTT+AGG + Chr2:25578613-25578632 MsG0280008109.01.T01:CDS 25.0%
!!! GTTATTATTGATTTTCAAGG+TGG + Chr2:25576563-25576582 MsG0280008109.01.T01:CDS 25.0%
!!! TACTAATGGTTTTTTCTTAG+GGG + Chr2:25578615-25578634 MsG0280008109.01.T01:CDS 25.0%
!!! TTGTACTTTTTCGCTTTTTT+GGG - Chr2:25576837-25576856 None:intergenic 25.0%
AATCAATAATAACACCTCCA+AGG - Chr2:25576556-25576575 None:intergenic 30.0%
ACTCTCACCAAACAATATAA+AGG + Chr2:25577326-25577345 MsG0280008109.01.T01:intron 30.0%
CCAATTGTAAAATTAGATGG+GGG + Chr2:25578730-25578749 MsG0280008109.01.T01:CDS 30.0%
CCTCTTCCAAAATATATACA+TGG - Chr2:25577704-25577723 None:intergenic 30.0%
GAAGGAATTAAAGTTCAAAG+TGG + Chr2:25578265-25578284 MsG0280008109.01.T01:CDS 30.0%
TAATTCCTTCTGTGTTGAAA+TGG - Chr2:25578255-25578274 None:intergenic 30.0%
TACTTGATATGAAGGTTTCA+TGG - Chr2:25578291-25578310 None:intergenic 30.0%
TATGAACAATGTGATTGGTA+TGG + Chr2:25578783-25578802 MsG0280008109.01.T01:CDS 30.0%
TTACAGCTAGGAAAAAATTG+AGG - Chr2:25576951-25576970 None:intergenic 30.0%
TTAGTAACATGAACTTGAAC+AGG - Chr2:25578601-25578620 None:intergenic 30.0%
TTGATGCACTTAATTCAACA+AGG - Chr2:25578054-25578073 None:intergenic 30.0%
TTGTTCAAGTAATCTGTTGT+TGG - Chr2:25578110-25578129 None:intergenic 30.0%
TTTACATGCACACAAATGAA+GGG - Chr2:25575913-25575932 None:intergenic 30.0%
! AACTGATGTTTGTTTCTTAG+CGG - Chr2:25576872-25576891 None:intergenic 30.0%
! AAGAGAATATTAGAGAGTGT+TGG - Chr2:25577113-25577132 None:intergenic 30.0%
! ACTTTTACATCAATGACCAA+AGG + Chr2:25577977-25577996 MsG0280008109.01.T01:CDS 30.0%
! AGTGGGTTCAAAATTATTAG+AGG - Chr2:25577309-25577328 None:intergenic 30.0%
! ATGGAGTATTAAGAAATTGC+AGG - Chr2:25577267-25577286 None:intergenic 30.0%
! CAAAAAAGCGAAAAAGTACA+AGG + Chr2:25576836-25576855 MsG0280008109.01.T01:intron 30.0%
! GTATTTTGTTGAAGTTAGCA+GGG + Chr2:25578515-25578534 MsG0280008109.01.T01:CDS 30.0%
! GTTCAAGTTCATGTTACTAA+TGG + Chr2:25578601-25578620 MsG0280008109.01.T01:CDS 30.0%
! TCTCAAGGTATTTATCTTGT+TGG - Chr2:25577355-25577374 None:intergenic 30.0%
! TTATATTGTTTGGTGAGAGT+GGG - Chr2:25577326-25577345 None:intergenic 30.0%
! TTTATATTGTTTGGTGAGAG+TGG - Chr2:25577327-25577346 None:intergenic 30.0%
! TTTTACATGCACACAAATGA+AGG - Chr2:25575914-25575933 None:intergenic 30.0%
!! CCATGTATATATTTTGGAAG+AGG + Chr2:25577701-25577720 MsG0280008109.01.T01:intron 30.0%
!! GGGTGATTTTTCAAATGTTT+TGG + Chr2:25578861-25578880 MsG0280008109.01.T01:CDS 30.0%
!!! ACCTTTTTTTTGTGTGAAGA+TGG - Chr2:25576129-25576148 None:intergenic 30.0%
!!! ATGTTTTGGTGTTTCCTAAT+AGG + Chr2:25578875-25578894 MsG0280008109.01.T01:CDS 30.0%
!!! CTTGTACTTTTTCGCTTTTT+TGG - Chr2:25576838-25576857 None:intergenic 30.0%
AAAATGTTCCAGAAGCTCTT+AGG - Chr2:25578016-25578035 None:intergenic 35.0%
AAATGTTCCAGAAGCTCTTA+GGG - Chr2:25578015-25578034 None:intergenic 35.0%
ACTGAGTTTGCAACAAACTT+TGG - Chr2:25578940-25578959 None:intergenic 35.0%
AGCTTCTACAGCTTTCATAA+TGG - Chr2:25576483-25576502 None:intergenic 35.0%
CATCAATGACCAAAGGTAAA+CGG + Chr2:25577984-25578003 MsG0280008109.01.T01:CDS 35.0%
CTCACCAAACAATATAAAGG+TGG + Chr2:25577329-25577348 MsG0280008109.01.T01:intron 35.0%
GATCTGCACCATACTTAATT+GGG - Chr2:25576433-25576452 None:intergenic 35.0%
GATTGGAAAATGAAGAAGAG+TGG + Chr2:25576395-25576414 MsG0280008109.01.T01:intron 35.0%
GCCATCTTCACACAAAAAAA+AGG + Chr2:25576125-25576144 MsG0280008109.01.T01:CDS 35.0%
TCCTCTAACAACAATTCCAA+TGG - Chr2:25578434-25578453 None:intergenic 35.0%
TTACAATCTCATTCTCTCTG+TGG + Chr2:25576367-25576386 MsG0280008109.01.T01:intron 35.0%
TTACATGCACACAAATGAAG+GGG - Chr2:25575912-25575931 None:intergenic 35.0%
TTAGGGCTTGAATTATTACC+AGG + Chr2:25576521-25576540 MsG0280008109.01.T01:CDS 35.0%
TTGAGTGAGACTTACATTGA+TGG - Chr2:25577286-25577305 None:intergenic 35.0%
TTGGCCACCTTTATATTGTT+TGG - Chr2:25577336-25577355 None:intergenic 35.0%
! AAAGGCGAAAGACAATTTTC+GGG + Chr2:25578346-25578365 MsG0280008109.01.T01:CDS 35.0%
! ACTGATGTTTGTTTCTTAGC+GGG - Chr2:25576871-25576890 None:intergenic 35.0%
! ATGCTTTTGTTGCATTTTGG+AGG - Chr2:25578087-25578106 None:intergenic 35.0%
! CAAAGGCGAAAGACAATTTT+CGG + Chr2:25578345-25578364 MsG0280008109.01.T01:CDS 35.0%
! CCCCCATCTAATTTTACAAT+TGG - Chr2:25578733-25578752 None:intergenic 35.0%
! GGTATTTTGTTGAAGTTAGC+AGG + Chr2:25578514-25578533 MsG0280008109.01.T01:CDS 35.0%
! TGCTTTTGTTGCATTTTGGA+GGG - Chr2:25578086-25578105 None:intergenic 35.0%
! TGGATGCTTTTGTTGCATTT+TGG - Chr2:25578090-25578109 None:intergenic 35.0%
!! AACAAAGCAACATGTTTTGG+TGG + Chr2:25578487-25578506 MsG0280008109.01.T01:CDS 35.0%
!! ACTAAGGGTTCGTTTGTTAT+CGG + Chr2:25577604-25577623 MsG0280008109.01.T01:intron 35.0%
!!! AGCATTTCTCTTGTCACTTT+TGG + Chr2:25575999-25576018 MsG0280008109.01.T01:CDS 35.0%
!!! ATGGGGTTATTTTTTCCTGA+AGG - Chr2:25577019-25577038 None:intergenic 35.0%
AAAGCCTTGACCAAAATGTC+TGG + Chr2:25577380-25577399 MsG0280008109.01.T01:intron 40.0%
AAGGAAACAACACCATGAAC+TGG - Chr2:25579006-25579025 None:intergenic 40.0%
AAGTTGAATGTGGCTGGTAA+TGG + Chr2:25578826-25578845 MsG0280008109.01.T01:CDS 40.0%
AATGAAGAAGAGTGGAAGAG+TGG + Chr2:25576403-25576422 MsG0280008109.01.T01:intron 40.0%
AGCAGATGAAGGAAATGTGA+TGG - Chr2:25576609-25576628 None:intergenic 40.0%
ATCACATTTCCTTCATCTGC+TGG + Chr2:25576608-25576627 MsG0280008109.01.T01:CDS 40.0%
CAATGAATATTCCTCCACCT+CGG - Chr2:25578192-25578211 None:intergenic 40.0%
CCATAATATCAACCTCTTCG+AGG - Chr2:25576978-25576997 None:intergenic 40.0%
CTACTATCTCTTCTACCTTC+AGG + Chr2:25577001-25577020 MsG0280008109.01.T01:intron 40.0%
GAAAGTGAGAAACCAGTTCA+TGG + Chr2:25578991-25579010 MsG0280008109.01.T01:CDS 40.0%
GCAGATGAAGGAAATGTGAT+GGG - Chr2:25576608-25576627 None:intergenic 40.0%
GGAAGAAGAAAATAGCACTG+TGG - Chr2:25577922-25577941 None:intergenic 40.0%
GGATCTGCACCATACTTAAT+TGG - Chr2:25576434-25576453 None:intergenic 40.0%
GTGGTGTACCCAATTAAGTA+TGG + Chr2:25576422-25576441 MsG0280008109.01.T01:CDS 40.0%
TAATATCAACCTCTTCGAGG+TGG - Chr2:25576975-25576994 None:intergenic 40.0%
TACATGCACACAAATGAAGG+GGG - Chr2:25575911-25575930 None:intergenic 40.0%
TACGTGTTCATTGAAGCAAG+TGG - Chr2:25576046-25576065 None:intergenic 40.0%
TGCTGAGAAAATTGCGACAT+CGG - Chr2:25578410-25578429 None:intergenic 40.0%
TTACCAGGAATTAGAGACCT+TGG + Chr2:25576536-25576555 MsG0280008109.01.T01:CDS 40.0%
TTCCTCCATTTCAACACAGA+AGG + Chr2:25578247-25578266 MsG0280008109.01.T01:CDS 40.0%
TTCCTTCTGTGTTGAAATGG+AGG - Chr2:25578252-25578271 None:intergenic 40.0%
! CAATTGCTAGCCAGACATTT+TGG - Chr2:25577393-25577412 None:intergenic 40.0%
! GCCATTGGAATTGTTGTTAG+AGG + Chr2:25578430-25578449 MsG0280008109.01.T01:CDS 40.0%
! TCAACAAGGTGTCTTTCAGT+TGG - Chr2:25578040-25578059 None:intergenic 40.0%
! TCTCATTCTCTCTGTGGTTT+TGG + Chr2:25576373-25576392 MsG0280008109.01.T01:intron 40.0%
!! AGACATTTTGGTCAAGGCTT+TGG - Chr2:25577381-25577400 None:intergenic 40.0%
!! CCTCGAAGAGGTTGATATTA+TGG + Chr2:25576975-25576994 MsG0280008109.01.T01:intron 40.0%
!! TGTTGCATTTTGGAGGGTTT+TGG - Chr2:25578080-25578099 None:intergenic 40.0%
!!! ATGGTTTTGAGATCAACCGT+TGG + Chr2:25578802-25578821 MsG0280008109.01.T01:CDS 40.0%
!!! GCAACATGTTTTGGTGGAAT+TGG + Chr2:25578493-25578512 MsG0280008109.01.T01:CDS 40.0%
!!! TCAAGGCTTTGGTTTTCTCA+AGG - Chr2:25577370-25577389 None:intergenic 40.0%
!!! TGGTTTTGAGATCAACCGTT+GGG + Chr2:25578803-25578822 MsG0280008109.01.T01:CDS 40.0%
!!! TTCTCTCTGTGGTTTTGGAT+TGG + Chr2:25576378-25576397 MsG0280008109.01.T01:intron 40.0%
AACCGTTGGGAAGTTGAATG+TGG + Chr2:25578816-25578835 MsG0280008109.01.T01:CDS 45.0%
AAGTATGGTGCAGATCCAAC+TGG + Chr2:25576437-25576456 MsG0280008109.01.T01:CDS 45.0%
ACAAACGAACCCTTAGTCTC+AGG - Chr2:25577601-25577620 None:intergenic 45.0%
ACAAGATTGCCAGCAGATGA+AGG - Chr2:25576620-25576639 None:intergenic 45.0%
AGCCACATTCAACTTCCCAA+CGG - Chr2:25578821-25578840 None:intergenic 45.0%
ATTTGTGGTCCCTGAGACTA+AGG + Chr2:25577588-25577607 MsG0280008109.01.T01:intron 45.0%
CAAACGAACCCTTAGTCTCA+GGG - Chr2:25577600-25577619 None:intergenic 45.0%
CAATGACCAAAGGTAAACGG+AGG + Chr2:25577987-25578006 MsG0280008109.01.T01:CDS 45.0%
CTGCTTTCATTCACTCCAGT+TGG - Chr2:25576455-25576474 None:intergenic 45.0%
CTTGGACAACACTCAACAGT+TGG + Chr2:25578316-25578335 MsG0280008109.01.T01:CDS 45.0%
GGACAAGCTAACATGATCAC+AGG + Chr2:25578451-25578470 MsG0280008109.01.T01:CDS 45.0%
GTGTTTCCTAGTACGCTTCT+AGG - Chr2:25577241-25577260 None:intergenic 45.0%
TGTCTGGCTAGCAATTGTGT+TGG + Chr2:25577396-25577415 MsG0280008109.01.T01:intron 45.0%
TTCATCTGCTGGCAATCTTG+TGG + Chr2:25576619-25576638 MsG0280008109.01.T01:CDS 45.0%
TTTGTGGTCCCTGAGACTAA+GGG + Chr2:25577589-25577608 MsG0280008109.01.T01:intron 45.0%
! GCAATTTTCTCAGCAGCCAT+TGG + Chr2:25578415-25578434 MsG0280008109.01.T01:CDS 45.0%
! GGTAATGGCACAAAATGGGT+GGG + Chr2:25578841-25578860 MsG0280008109.01.T01:CDS 45.0%
!! CTAGCCAGACATTTTGGTCA+AGG - Chr2:25577387-25577406 None:intergenic 45.0%
!! GGCTGGTAATGGCACAAAAT+GGG + Chr2:25578837-25578856 MsG0280008109.01.T01:CDS 45.0%
!! TGGCTGGTAATGGCACAAAA+TGG + Chr2:25578836-25578855 MsG0280008109.01.T01:CDS 45.0%
!! TGGTAATGGCACAAAATGGG+TGG + Chr2:25578840-25578859 MsG0280008109.01.T01:CDS 45.0%
!! ATAATTTAAATAACATTATT+TGG - Chr2:25577043-25577062 None:intergenic 5.0%
!!! TTTTTTTTTATGATTTTAAT+TGG + Chr2:25576195-25576214 MsG0280008109.01.T01:intron 5.0%
AGCTGTAAACCACCTCGAAG+AGG + Chr2:25576963-25576982 MsG0280008109.01.T01:intron 50.0%
AGGGTTCCTCCGTTTACCTT+TGG - Chr2:25577996-25578015 None:intergenic 50.0%
CCAGGAATTAGAGACCTTGG+AGG + Chr2:25576539-25576558 MsG0280008109.01.T01:CDS 50.0%
CCTAGTACGCTTCTAGGTAC+AGG - Chr2:25577235-25577254 None:intergenic 50.0%
CCTCCAAGGTCTCTAATTCC+TGG - Chr2:25576542-25576561 None:intergenic 50.0%
CTTCGAGGTGGTTTACAGCT+AGG - Chr2:25576963-25576982 None:intergenic 50.0%
GGACAACACTCAACAGTTGG+CGG + Chr2:25578319-25578338 MsG0280008109.01.T01:CDS 50.0%
GTGTGAAGATGGCTGAGACA+TGG - Chr2:25576118-25576137 None:intergenic 50.0%
GTTGGGAAGTTGAATGTGGC+TGG + Chr2:25578820-25578839 MsG0280008109.01.T01:CDS 50.0%
TCAACAGTTGGCGGAGACAA+AGG + Chr2:25578328-25578347 MsG0280008109.01.T01:CDS 50.0%
! CCTGTACCTAGAAGCGTACT+AGG + Chr2:25577232-25577251 MsG0280008109.01.T01:CDS 50.0%
CTCGGTAGCTCGAATCGAAG+AGG - Chr2:25578174-25578193 None:intergenic 55.0%
GGAGGAACCCTAAGAGCTTC+TGG + Chr2:25578005-25578024 MsG0280008109.01.T01:CDS 55.0%
! CTCTTCGATTCGAGCTACCG+AGG + Chr2:25578172-25578191 MsG0280008109.01.T01:CDS 55.0%
! TTCGATTCGAGCTACCGAGG+TGG + Chr2:25578175-25578194 MsG0280008109.01.T01:CDS 55.0%
GATTCGAGCTACCGAGGTGG+AGG + Chr2:25578178-25578197 MsG0280008109.01.T01:CDS 60.0%
Chromosome Type Strat End Strand Name
Chr2 gene 25575886 25579041 25575886 ID=MsG0280008109.01;Name=MsG0280008109.01
Chr2 mRNA 25575886 25579041 25575886 ID=MsG0280008109.01.T01;Parent=MsG0280008109.01;Name=MsG0280008109.01.T01;_AED=0.44;_eAED=0.44;_QI=87|0.33|0.5|0.75|0.66|0.5|4|0|526
Chr2 exon 25575886 25576146 25575886 ID=MsG0280008109.01.T01:exon:14056;Parent=MsG0280008109.01.T01
Chr2 exon 25576410 25576640 25576410 ID=MsG0280008109.01.T01:exon:14057;Parent=MsG0280008109.01.T01
Chr2 exon 25577232 25577290 25577232 ID=MsG0280008109.01.T01:exon:14058;Parent=MsG0280008109.01.T01
Chr2 exon 25577925 25579041 25577925 ID=MsG0280008109.01.T01:exon:14059;Parent=MsG0280008109.01.T01
Chr2 five_prime_UTR 25575886 25575972 25575886 ID=MsG0280008109.01.T01:five_prime_utr;Parent=MsG0280008109.01.T01
Chr2 CDS 25575973 25576146 25575973 ID=MsG0280008109.01.T01:cds;Parent=MsG0280008109.01.T01
Chr2 CDS 25576410 25576640 25576410 ID=MsG0280008109.01.T01:cds;Parent=MsG0280008109.01.T01
Chr2 CDS 25577232 25577290 25577232 ID=MsG0280008109.01.T01:cds;Parent=MsG0280008109.01.T01
Chr2 CDS 25577925 25579041 25577925 ID=MsG0280008109.01.T01:cds;Parent=MsG0280008109.01.T01
Gene Sequence

>MsG0280008109.01.T01

ATGTTTGCTATGAGATTCTCAACGTTAGCATTTCTCTTGTCACTTTTGGTGATACTACAAGTACAAGAAACCACTTGCTTCAATGAACACGTAGAACTTTCTCATCTTGTGAAAAAAATTGTCAAGCAAAAAATGGACATTGCCATGTCTCAGCCATCTTCACACAAAAAAAAGAAGAGTGGAAGAGTGGTGTACCCAATTAAGTATGGTGCAGATCCAACTGGAGTGAATGAAAGCAGTGATGCCATTATGAAAGCTGTAGAAGCTGCATTTGATATTGATAATTTAGGGCTTGAATTATTACCAGGAATTAGAGACCTTGGAGGTGTTATTATTGATTTTCAAGGTGGAAACTATAAAATCAGCAATCCCATCACATTTCCTTCATCTGCTGGCAATCTTGTGCCTGTACCTAGAAGCGTACTAGGAAACACTATCCTGCAATTTCTTAATACTCCATCAATTGCTATTTTCTTCTTCCACTTAACTGTTACATATCAATATTTCTTACACTTAACTTTTACATCAATGACCAAAGGTAAACGGAGGAACCCTAAGAGCTTCTGGAACATTTTTCCAACTGAAAGACACCTTGTTGAATTAAGTGCATCAAATTCCAAAACCCTCCAAAATGCAACAAAAGCATCCAACAACAGATTACTTGAACAAACAATAGGAATTTACTATGAAGACATAACATTTAGAGACATCCTCTTCGATTCGAGCTACCGAGGTGGAGGAATATTCATTGTCGATTCAGCTAGAATCAGAATCGACAATTGTTTCTTCCTCCATTTCAACACAGAAGGAATTAAAGTTCAAAGTGGCCATGAAACCTTCATATCAAGTAGTTTTCTTGGACAACACTCAACAGTTGGCGGAGACAAAGGCGAAAGACAATTTTCGGGAACTGCAATTGATCTTGCTAGCAATGACAATGCTATCACCGATGTCGCAATTTTCTCAGCAGCCATTGGAATTGTTGTTAGAGGACAAGCTAACATGATCACAGGTGTGCATTGTTATAACAAAGCAACATGTTTTGGTGGAATTGGTATTTTGTTGAAGTTAGCAGGGAACTCACAAACAAGAATTGATAACTGTTACATGGATTATAATTCTATAGTCATGGAAGATCCTGTTCAAGTTCATGTTACTAATGGTTTTTTCTTAGGGGATGCTAATATTGTTTTGAAGTCAATTAAAGGGAAAGTTTATGGTTTAAATATTGTTAACAATATGTTTAGTGGAAACCCTAATAATAATGTTCCAATTGTAAAATTAGATGGGGGATTTAGTAACATTGATCAAGTGGTGATTGATATGAACAATGTGATTGGTATGGTTTTGAGATCAACCGTTGGGAAGTTGAATGTGGCTGGTAATGGCACAAAATGGGTGGGTGATTTTTCAAATGTTTTGGTGTTTCCTAATAGGATTAGTAATTTTCAGTATTCATTTCATACATTAGAGGGACCAAAGTTTGTTGCAAACTCAGTGAGTAATGTGTCAAATAATGTTGTAGTTGTTGAAAGTGAGAAACCAGTTCATGGTGTTGTTTCCTTCTTTGTTGAACAATGA
Protein sequence

>MsG0280008109.01.T01

MFAMRFSTLAFLLSLLVILQVQETTCFNEHVELSHLVKKIVKQKMDIAMSQPSSHKKKKSGRVVYPIKYGADPTGVNESSDAIMKAVEAAFDIDNLGLELLPGIRDLGGVIIDFQGGNYKISNPITFPSSAGNLVPVPRSVLGNTILQFLNTPSIAIFFFHLTVTYQYFLHLTFTSMTKGKRRNPKSFWNIFPTERHLVELSASNSKTLQNATKASNNRLLEQTIGIYYEDITFRDILFDSSYRGGGIFIVDSARIRIDNCFFLHFNTEGIKVQSGHETFISSSFLGQHSTVGGDKGERQFSGTAIDLASNDNAITDVAIFSAAIGIVVRGQANMITGVHCYNKATCFGGIGILLKLAGNSQTRIDNCYMDYNSIVMEDPVQVHVTNGFFLGDANIVLKSIKGKVYGLNIVNNMFSGNPNNNVPIVKLDGGFSNIDQVVIDMNNVIGMVLRSTVGKLNVAGNGTKWVGDFSNVLVFPNRISNFQYSFHTLEGPKFVANSVSNVSNNVVVVESEKPVHGVVSFFVEQ*