AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar ZhongmuNo.1 / MsG0280008110.01

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MsG0280008110.01.T01 MTR_2g035790 89.139 488 43 1 4 481 1 488 0.0 855
MsG0280008110.01.T01 MTR_4g094732 67.174 460 146 3 25 481 23 480 0.0 618
MsG0280008110.01.T01 MTR_2g035760 51.574 413 182 7 62 469 82 481 3.21e-139 410
MsG0280008110.01.T01 MTR_6g015785 51.799 417 178 7 69 481 93 490 3.91e-132 392
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MsG0280008110.01.T01 AT4G20040 62.676 426 152 5 58 481 63 483 0.0 545
MsG0280008110.01.T01 AT4G20050 48.354 486 222 8 6 481 13 479 6.94e-147 429
MsG0280008110.01.T01 AT4G20050 48.354 486 222 8 6 481 13 479 6.94e-147 429
MsG0280008110.01.T01 AT4G20050 48.354 486 222 8 6 481 13 479 6.94e-147 429
MsG0280008110.01.T01 AT4G20050 48.354 486 222 8 6 481 13 479 6.94e-147 429
MsG0280008110.01.T01 AT4G20050 48.354 486 222 8 6 481 38 504 1.15e-146 429
MsG0280008110.01.T01 AT4G20050 53.000 400 164 7 87 481 1 381 1.14e-133 391
MsG0280008110.01.T01 AT4G20050 53.000 400 164 7 87 481 1 381 1.14e-133 391

Find 91 sgRNAs with CRISPR-Local

Find 205 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
GCATTTGATATTGATAATTT+AGG 0.107857 2:+25582552 MsG0280008110.01.T01:CDS
ACAATTGGAACATTATTATT+AGG 0.129299 2:-25585184 None:intergenic
GGATCTGCACCATACTTAAT+TGG 0.153680 2:-25582480 None:intergenic
TCAATTAAAGGGAAAGTTTA+TGG 0.171584 2:+25585127 MsG0280008110.01.T01:CDS
CAGCCATTGGAATTGTTCTT+AGG 0.195596 2:+25584897 MsG0280008110.01.T01:CDS
AGCATTTCTCTTGTCACTTT+TGG 0.197047 2:+25582042 MsG0280008110.01.T01:CDS
CATTTGATATTGATAATTTA+GGG 0.251344 2:+25582553 MsG0280008110.01.T01:CDS
GTTCCAATTGTAAAATTAGA+TGG 0.268649 2:+25585196 MsG0280008110.01.T01:CDS
CATCAATGACCAAAGGTAAA+TGG 0.278889 2:+25584455 MsG0280008110.01.T01:intron
CAATTGGAACATTATTATTA+GGG 0.285274 2:-25585183 None:intergenic
TATAACAAAGCAACAGGTTT+TGG 0.286379 2:+25584953 MsG0280008110.01.T01:CDS
TGAAAATGACTAATCCTATT+AGG 0.294977 2:-25585358 None:intergenic
ATTGTTAACAATATGTTTAG+TGG 0.301676 2:+25585157 MsG0280008110.01.T01:CDS
TGGATGGTAATGGCACAAAA+TGG 0.367127 2:+25585305 MsG0280008110.01.T01:CDS
TAATTCCTTCTGTGTTGAAA+TGG 0.369391 2:-25584721 None:intergenic
AAATGTTTCAGAAGCTCTTA+AGG 0.373297 2:-25584483 None:intergenic
TACTTGATATGAAGGTTTCA+TGG 0.388165 2:-25584757 None:intergenic
CCTCCAAGGTCTCTAATTCC+TGG 0.397675 2:-25582588 None:intergenic
CATTGTTATAACAAAGCAAC+AGG 0.404226 2:+25584947 MsG0280008110.01.T01:CDS
TTCGTAACATGAACTTGAAC+AGG 0.426663 2:-25585067 None:intergenic
AGCCATTGGAATTGTTCTTA+GGG 0.433955 2:+25584898 MsG0280008110.01.T01:CDS
ACTGAATGTGCAACAAACTT+TGG 0.434126 2:-25585406 None:intergenic
AAGAAAACTACTTGATATGA+AGG 0.434177 2:-25584765 None:intergenic
AGCTTCTACAGCTTTCATAA+TGG 0.438110 2:-25582529 None:intergenic
AAGTATGGTGCAGATCCAAC+AGG 0.439437 2:+25582486 MsG0280008110.01.T01:CDS
GATCTGCACCATACTTAATT+GGG 0.441924 2:-25582479 None:intergenic
TTGTTCAAGTAATCTGTTGT+TGG 0.449557 2:-25584576 None:intergenic
ATCACATTTCCTTCATCGGC+TGG 0.454793 2:+25582657 MsG0280008110.01.T01:CDS
TTCCAATTGTAAAATTAGAT+GGG 0.461356 2:+25585197 MsG0280008110.01.T01:CDS
GTATTCATTTCATACATTAG+AGG 0.463619 2:+25585381 MsG0280008110.01.T01:CDS
TCCCATCACATTTCCTTCAT+CGG 0.471050 2:+25582653 MsG0280008110.01.T01:CDS
CTCGGTAGCTTGAATCGAAA+AGG 0.482576 2:-25584640 None:intergenic
AAAGAAACAACACCATGAAC+TGG 0.487041 2:-25585472 None:intergenic
TATTCATTTCATACATTAGA+GGG 0.494492 2:+25585382 MsG0280008110.01.T01:CDS
TTACCAGGAATTAGAGACCT+TGG 0.494991 2:+25582585 MsG0280008110.01.T01:CDS
TTCCTCCATTTCAACACAGA+AGG 0.508555 2:+25584716 MsG0280008110.01.T01:CDS
TGAGTTTGAGATCAACGGTT+GGG 0.510003 2:+25585272 MsG0280008110.01.T01:CDS
AAGAATTGATAATTGTTACA+TGG 0.510868 2:+25585018 MsG0280008110.01.T01:CDS
TTAGGGCTTGAATTATTACC+AGG 0.514044 2:+25582570 MsG0280008110.01.T01:CDS
TTCTACGTGTTCATTGAAGC+AGG 0.514067 2:-25582089 None:intergenic
TCAACAAGATGTCTATCAGT+TGG 0.517912 2:-25584506 None:intergenic
GTGGTGTACCCAATTAAGTA+TGG 0.526549 2:+25582471 MsG0280008110.01.T01:CDS
ACAAGATTGCCAGCCGATGA+AGG 0.526613 2:-25582666 None:intergenic
GTTCAAGTTCATGTTACGAA+TGG 0.528355 2:+25585070 MsG0280008110.01.T01:CDS
GAAAGTGAGAAACCAGTTCA+TGG 0.531426 2:+25585460 MsG0280008110.01.T01:CDS
GATTCAAGCTACCGAGGTGG+AGG 0.532692 2:+25584647 MsG0280008110.01.T01:CDS
GATTGGAAAATGAAGAAGAG+TGG 0.536042 2:+25582444 MsG0280008110.01.T01:intron
CTTGGACAACACTCAACAGT+CGG 0.538774 2:+25584785 MsG0280008110.01.T01:CDS
TGGTAATGGCACAAAATGGG+TGG 0.549729 2:+25585309 MsG0280008110.01.T01:CDS
TTCCTTCTGTGTTGAAATGG+AGG 0.550409 2:-25584718 None:intergenic
GGATGGTAATGGCACAAAAT+GGG 0.551911 2:+25585306 MsG0280008110.01.T01:CDS
AGTGATGACAATTTATGTAG+AGG 0.554069 2:-25581928 None:intergenic
AAAGACGAAAGACAATTCTC+AGG 0.562130 2:+25584815 MsG0280008110.01.T01:CDS
GGATTATAATTCTATAGTCA+TGG 0.563335 2:+25585039 MsG0280008110.01.T01:CDS
TTCATCGGCTGGCAATCTTG+TGG 0.566505 2:+25582668 MsG0280008110.01.T01:CDS
TCCAATTGTAAAATTAGATG+GGG 0.567825 2:+25585198 MsG0280008110.01.T01:CDS
AATGAAGAAGAGTGGAAGAG+TGG 0.571785 2:+25582452 MsG0280008110.01.T01:intron
GCCGATGAAGGAAATGTGAT+GGG 0.577733 2:-25582654 None:intergenic
ATTTAGTAACATTGATCAAG+TGG 0.578299 2:+25585222 MsG0280008110.01.T01:CDS
ATGAGTTTGAGATCAACGGT+TGG 0.587308 2:+25585271 MsG0280008110.01.T01:CDS
CCAGGAATTAGAGACCTTGG+AGG 0.587401 2:+25582588 MsG0280008110.01.T01:CDS
AAGTGACAAGAGAAATGCTA+AGG 0.588861 2:-25582038 None:intergenic
GGACAAGCTAACATGATCAC+AGG 0.590351 2:+25584920 MsG0280008110.01.T01:CDS
TGGCATGAGTTTGAGATCAA+CGG 0.596349 2:+25585267 MsG0280008110.01.T01:CDS
GCCATTGGAATTGTTCTTAG+GGG 0.599615 2:+25584899 MsG0280008110.01.T01:CDS
TGCCGAGAAAATTGCAACAT+CGG 0.599656 2:-25584876 None:intergenic
TGTTGAACAATGAATTAACA+TGG 0.602500 2:+25585498 MsG0280008110.01.T01:exon
TACGTGTTCATTGAAGCAGG+TGG 0.603710 2:-25582086 None:intergenic
CCAATTGTAAAATTAGATGG+GGG 0.605323 2:+25585199 MsG0280008110.01.T01:CDS
AGCCGATGAAGGAAATGTGA+TGG 0.612995 2:-25582655 None:intergenic
TCCCCTAAGAACAATTCCAA+TGG 0.613399 2:-25584900 None:intergenic
AAGTTGAATGTGGATGGTAA+TGG 0.619073 2:+25585295 MsG0280008110.01.T01:CDS
TTTCATGCACACAAATGAAG+GGG 0.621493 2:-25581952 None:intergenic
TTCGATTCAAGCTACCGAGG+TGG 0.625847 2:+25584644 MsG0280008110.01.T01:CDS
ATTGATATGAACAATGTGGT+TGG 0.625929 2:+25585247 MsG0280008110.01.T01:CDS
AATCAATGATAACACCTCCA+AGG 0.627415 2:-25582602 None:intergenic
TTCATGCACACAAATGAAGG+GGG 0.634524 2:-25581951 None:intergenic
GTGTGAAGATGGCTGAGACA+TGG 0.634929 2:-25582158 None:intergenic
AGATTACTTGAACAAACGAT+AGG 0.635310 2:+25584584 MsG0280008110.01.T01:CDS
GAAGGAATTAAAGTTCAAAG+TGG 0.637402 2:+25584734 MsG0280008110.01.T01:CDS
GTTGGGAAGTTGAATGTGGA+TGG 0.638186 2:+25585289 MsG0280008110.01.T01:CDS
AACGGTTGGGAAGTTGAATG+TGG 0.640437 2:+25585285 MsG0280008110.01.T01:CDS
GGTAATGGCACAAAATGGGT+GGG 0.641219 2:+25585310 MsG0280008110.01.T01:CDS
GTTGCTATCAAGATCAATTG+CGG 0.644562 2:-25584840 None:intergenic
AGTATGGTGCAGATCCAACA+GGG 0.649273 2:+25582487 MsG0280008110.01.T01:CDS
ATTGTCGATTCTGATTCTAG+CGG 0.659026 2:-25584687 None:intergenic
CAATGACCAAAGGTAAATGG+AGG 0.667241 2:+25584458 MsG0280008110.01.T01:intron
GGACAACACTCAACAGTCGG+TGG 0.682248 2:+25584788 MsG0280008110.01.T01:CDS
GGTAATTGATATGAACAATG+TGG 0.715658 2:+25585243 MsG0280008110.01.T01:CDS
CGATGAATATTCCTCCACCT+CGG 0.718566 2:-25584658 None:intergenic
GTATGGTGCAGATCCAACAG+GGG 0.763386 2:+25582488 MsG0280008110.01.T01:CDS

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!! CTAATTTATATAACAAATTA+AGG - Chr2:25582793-25582812 None:intergenic 10.0%
!! TCAAAATAATAAATTTGTAT+TGG + Chr2:25583912-25583931 MsG0280008110.01.T01:intron 10.0%
!!! ATGAGATTTTAAAAAAATTA+TGG + Chr2:25583575-25583594 MsG0280008110.01.T01:intron 10.0%
!!! ATTTAATTTGTAATTAAACA+TGG + Chr2:25583695-25583714 MsG0280008110.01.T01:intron 10.0%
!!! GAATTTTTTTTTTTTGAATA+AGG + Chr2:25583777-25583796 MsG0280008110.01.T01:intron 10.0%
!!! TTTTTTTTGATGATTTTAAT+TGG + Chr2:25582243-25582262 MsG0280008110.01.T01:intron 10.0%
!! AAAAATTGTTAAGCAAAAAA+TGG + Chr2:25582129-25582148 MsG0280008110.01.T01:CDS 15.0%
!! AAAATTGTTAAGCAAAAAAT+GGG + Chr2:25582130-25582149 MsG0280008110.01.T01:CDS 15.0%
!! AATAATATGTAAAAGTTAAG+TGG - Chr2:25584414-25584433 None:intergenic 15.0%
!! TGTGAATTTAATGATAAATA+AGG - Chr2:25584129-25584148 None:intergenic 15.0%
!!! AAATAAAACTTTATTTGGAA+TGG - Chr2:25583157-25583176 None:intergenic 15.0%
!!! AATAAAACTTTATTTGGAAT+GGG - Chr2:25583156-25583175 None:intergenic 15.0%
!!! CATTTGATATTGATAATTTA+GGG + Chr2:25582553-25582572 MsG0280008110.01.T01:CDS 15.0%
!!! CATTTTATACAATGAAAAAA+AGG - Chr2:25582389-25582408 None:intergenic 15.0%
!!! CTCTTTTGATTTTTTTTTAT+TGG + Chr2:25583281-25583300 MsG0280008110.01.T01:intron 15.0%
!! AAACATCAAAAGAAAAAGAA+TGG + Chr2:25584277-25584296 MsG0280008110.01.T01:intron 20.0%
!! AATCATCAAAAAAAATTCCT+AGG - Chr2:25582238-25582257 None:intergenic 20.0%
!! ACAATTGGAACATTATTATT+AGG - Chr2:25585187-25585206 None:intergenic 20.0%
!! ACATCTACTTTAATAGTTTA+TGG + Chr2:25584167-25584186 MsG0280008110.01.T01:intron 20.0%
!! AGCTAAAACATCAAAATAAA+AGG - Chr2:25584042-25584061 None:intergenic 20.0%
!! ATCAAAATAAAAGGAATGAA+GGG - Chr2:25584033-25584052 None:intergenic 20.0%
!! ATTGTTAACAATATGTTTAG+TGG + Chr2:25585157-25585176 MsG0280008110.01.T01:CDS 20.0%
!! CAATTGGAACATTATTATTA+GGG - Chr2:25585186-25585205 None:intergenic 20.0%
!! CATAACATAACAATATTTGA+GGG + Chr2:25584334-25584353 MsG0280008110.01.T01:intron 20.0%
!! TATTCATTTCATACATTAGA+GGG + Chr2:25585382-25585401 MsG0280008110.01.T01:CDS 20.0%
!! TCATAACATAACAATATTTG+AGG + Chr2:25584333-25584352 MsG0280008110.01.T01:intron 20.0%
!! TCGATTCAATAGAATAAAAA+AGG + Chr2:25583534-25583553 MsG0280008110.01.T01:intron 20.0%
!! TTCCAATTGTAAAATTAGAT+GGG + Chr2:25585197-25585216 MsG0280008110.01.T01:CDS 20.0%
!!! AAGAATTGATAATTGTTACA+TGG + Chr2:25585018-25585037 MsG0280008110.01.T01:CDS 20.0%
!!! AGTTGCAAAATTTTATTGAA+AGG - Chr2:25583986-25584005 None:intergenic 20.0%
!!! ATAAAACTTTATTTGGAATG+GGG - Chr2:25583155-25583174 None:intergenic 20.0%
!!! ATTGTTTTGAAGTCAATTAA+AGG + Chr2:25585115-25585134 MsG0280008110.01.T01:CDS 20.0%
!!! GCATTTGATATTGATAATTT+AGG + Chr2:25582552-25582571 MsG0280008110.01.T01:CDS 20.0%
!!! GTTATGTATGATTTTGATTT+TGG - Chr2:25581980-25581999 None:intergenic 20.0%
!!! TTAACTAAGTATTTGTTTTC+TGG - Chr2:25582986-25583005 None:intergenic 20.0%
!!! TTGTTTTGAAGTCAATTAAA+GGG + Chr2:25585116-25585135 MsG0280008110.01.T01:CDS 20.0%
! AAAAAAAATTCCTAGGAAGA+AGG - Chr2:25582231-25582250 None:intergenic 25.0%
! AAGAAAACTACTTGATATGA+AGG - Chr2:25584768-25584787 None:intergenic 25.0%
! AAGAGTTAAAACAAAGAAGA+GGG + Chr2:25583004-25583023 MsG0280008110.01.T01:intron 25.0%
! AGAACTCGAAAAAAGTAAAA+GGG + Chr2:25582948-25582967 MsG0280008110.01.T01:intron 25.0%
! ATCAAAAGAAAAAGAATGGA+GGG + Chr2:25584281-25584300 MsG0280008110.01.T01:intron 25.0%
! ATTTAGTAACATTGATCAAG+TGG + Chr2:25585222-25585241 MsG0280008110.01.T01:CDS 25.0%
! CATCAAAATAAAAGGAATGA+AGG - Chr2:25584034-25584053 None:intergenic 25.0%
! GGATTATAATTCTATAGTCA+TGG + Chr2:25585039-25585058 MsG0280008110.01.T01:CDS 25.0%
! GTATTCATTTCATACATTAG+AGG + Chr2:25585381-25585400 MsG0280008110.01.T01:CDS 25.0%
! GTTCCAATTGTAAAATTAGA+TGG + Chr2:25585196-25585215 MsG0280008110.01.T01:CDS 25.0%
! TAAGAGTTAAAACAAAGAAG+AGG + Chr2:25583003-25583022 MsG0280008110.01.T01:intron 25.0%
! TCAATTAAAGGGAAAGTTTA+TGG + Chr2:25585127-25585146 MsG0280008110.01.T01:CDS 25.0%
! TCCAATTGTAAAATTAGATG+GGG + Chr2:25585198-25585217 MsG0280008110.01.T01:CDS 25.0%
! TGAAAATGACTAATCCTATT+AGG - Chr2:25585361-25585380 None:intergenic 25.0%
! TGTTGAACAATGAATTAACA+TGG + Chr2:25585498-25585517 MsG0280008110.01.T01:exon 25.0%
! TTCTATGATGTACATATGTT+AGG + Chr2:25583885-25583904 MsG0280008110.01.T01:intron 25.0%
!! ATATTATTTTCCTTCTTCCT+AGG + Chr2:25582218-25582237 MsG0280008110.01.T01:intron 25.0%
!! CACTAAAACATCAAAAGTTT+TGG + Chr2:25584232-25584251 MsG0280008110.01.T01:intron 25.0%
!! CATTTTTCTAACATTGTTTG+AGG + Chr2:25582314-25582333 MsG0280008110.01.T01:intron 25.0%
!! TTCATATCAAGTAGTTTTCT+TGG + Chr2:25584767-25584786 MsG0280008110.01.T01:CDS 25.0%
!!! TTACGAATGGTTTTTTCTTA+GGG + Chr2:25585083-25585102 MsG0280008110.01.T01:CDS 25.0%
AAATGTTTCAGAAGCTCTTA+AGG - Chr2:25584486-25584505 None:intergenic 30.0%
AGATTACTTGAACAAACGAT+AGG + Chr2:25584584-25584603 MsG0280008110.01.T01:CDS 30.0%
ATCAATCAAAGTAGTAAAGG+AGG - Chr2:25582729-25582748 None:intergenic 30.0%
ATTAAAGTAGATGTGACCTT+TGG - Chr2:25584161-25584180 None:intergenic 30.0%
ATTCACATGTGATAAACCAA+AGG + Chr2:25584142-25584161 MsG0280008110.01.T01:intron 30.0%
ATTGATATGAACAATGTGGT+TGG + Chr2:25585247-25585266 MsG0280008110.01.T01:CDS 30.0%
CAGAACTCGAAAAAAGTAAA+AGG + Chr2:25582947-25582966 MsG0280008110.01.T01:intron 30.0%
CATCAAAAGAAAAAGAATGG+AGG + Chr2:25584280-25584299 MsG0280008110.01.T01:intron 30.0%
CATTGTTATAACAAAGCAAC+AGG + Chr2:25584947-25584966 MsG0280008110.01.T01:CDS 30.0%
CCAATTGTAAAATTAGATGG+GGG + Chr2:25585199-25585218 MsG0280008110.01.T01:CDS 30.0%
GAAGGAATTAAAGTTCAAAG+TGG + Chr2:25584734-25584753 MsG0280008110.01.T01:CDS 30.0%
GGTAATTGATATGAACAATG+TGG + Chr2:25585243-25585262 MsG0280008110.01.T01:CDS 30.0%
TAATTCCTTCTGTGTTGAAA+TGG - Chr2:25584724-25584743 None:intergenic 30.0%
TACTTGATATGAAGGTTTCA+TGG - Chr2:25584760-25584779 None:intergenic 30.0%
TCTCACCAAACAATACTAAA+GGG + Chr2:25583450-25583469 MsG0280008110.01.T01:intron 30.0%
TGCATCAATCAAAGTAGTAA+AGG - Chr2:25582732-25582751 None:intergenic 30.0%
TGGAGTTATTAAGAAATTGC+AGG - Chr2:25583388-25583407 None:intergenic 30.0%
TTGAGTGAGACTTAAATTGA+TGG - Chr2:25583408-25583427 None:intergenic 30.0%
TTGTTCAAGTAATCTGTTGT+TGG - Chr2:25584579-25584598 None:intergenic 30.0%
! ACTTTTACATCAATGACCAA+AGG + Chr2:25584448-25584467 MsG0280008110.01.T01:intron 30.0%
! CATTCAATGCAAGAACATTT+TGG - Chr2:25583309-25583328 None:intergenic 30.0%
! GAATTTTGTTGAAGTTAGCA+GGG + Chr2:25584984-25585003 MsG0280008110.01.T01:CDS 30.0%
! TATAACAAAGCAACAGGTTT+TGG + Chr2:25584953-25584972 MsG0280008110.01.T01:CDS 30.0%
! TTTATGGTGTTTTCCAACAA+GGG + Chr2:25584183-25584202 MsG0280008110.01.T01:intron 30.0%
! TTTTCATGCACACAAATGAA+GGG - Chr2:25581956-25581975 None:intergenic 30.0%
! TTTTCTTGAATGATCCTTGA+AGG - Chr2:25583127-25583146 None:intergenic 30.0%
! TTTTTCATGCACACAAATGA+AGG - Chr2:25581957-25581976 None:intergenic 30.0%
!! ACTAAGTATTTGTTTTCTGG+TGG - Chr2:25582983-25583002 None:intergenic 30.0%
!! CTAAGTATTTGTTTTCTGGT+GGG - Chr2:25582982-25583001 None:intergenic 30.0%
!! GATTTGTTCATTTTCCAACA+AGG - Chr2:25583512-25583531 None:intergenic 30.0%
!! GGGTGATTTTTCAAATGTTT+TGG + Chr2:25585330-25585349 MsG0280008110.01.T01:CDS 30.0%
!! TCACGTAGATATTTTTGAAG+AGG + Chr2:25583755-25583774 MsG0280008110.01.T01:intron 30.0%
!!! ACCTTTTTTTTGTGTGAAGA+TGG - Chr2:25582172-25582191 None:intergenic 30.0%
!!! ATGTTTTGGTGTTTCCTAAT+AGG + Chr2:25585344-25585363 MsG0280008110.01.T01:CDS 30.0%
!!! GGTGTTATCATTGATTTTCA+AGG + Chr2:25582609-25582628 MsG0280008110.01.T01:CDS 30.0%
!!! GTTACGAATGGTTTTTTCTT+AGG + Chr2:25585082-25585101 MsG0280008110.01.T01:CDS 30.0%
!!! GTTATCATTGATTTTCAAGG+TGG + Chr2:25582612-25582631 MsG0280008110.01.T01:CDS 30.0%
!!! TACGAATGGTTTTTTCTTAG+GGG + Chr2:25585084-25585103 MsG0280008110.01.T01:CDS 30.0%
AAAGAAACAACACCATGAAC+TGG - Chr2:25585475-25585494 None:intergenic 35.0%
AAAGACGAAAGACAATTCTC+AGG + Chr2:25584815-25584834 MsG0280008110.01.T01:CDS 35.0%
AAGTGACAAGAGAAATGCTA+AGG - Chr2:25582041-25582060 None:intergenic 35.0%
AAGTTGAATGTGGATGGTAA+TGG + Chr2:25585295-25585314 MsG0280008110.01.T01:CDS 35.0%
AATCAATGATAACACCTCCA+AGG - Chr2:25582605-25582624 None:intergenic 35.0%
ACAATCTCATTCTCTCTATG+TGG + Chr2:25582416-25582435 MsG0280008110.01.T01:intron 35.0%
ACTGAATGTGCAACAAACTT+TGG - Chr2:25585409-25585428 None:intergenic 35.0%
AGCCATTGGAATTGTTCTTA+GGG + Chr2:25584898-25584917 MsG0280008110.01.T01:CDS 35.0%
AGCTTCTACAGCTTTCATAA+TGG - Chr2:25582532-25582551 None:intergenic 35.0%
CAAAGTAGTAAAGGAGGAAA+AGG - Chr2:25582723-25582742 None:intergenic 35.0%
CATCAATGACCAAAGGTAAA+TGG + Chr2:25584455-25584474 MsG0280008110.01.T01:intron 35.0%
CTCACCAAACAATACTAAAG+GGG + Chr2:25583451-25583470 MsG0280008110.01.T01:intron 35.0%
CTCTCACCAAACAATACTAA+AGG + Chr2:25583449-25583468 MsG0280008110.01.T01:intron 35.0%
GAAGGGAGTAAGATGTTAAA+AGG - Chr2:25584016-25584035 None:intergenic 35.0%
GATCTGCACCATACTTAATT+GGG - Chr2:25582482-25582501 None:intergenic 35.0%
GATTGGAAAATGAAGAAGAG+TGG + Chr2:25582444-25582463 MsG0280008110.01.T01:intron 35.0%
GCACAAACCAGACAAAATAA+AGG + Chr2:25583645-25583664 MsG0280008110.01.T01:intron 35.0%
GCCATCTTCACACAAAAAAA+AGG + Chr2:25582168-25582187 MsG0280008110.01.T01:CDS 35.0%
GTTGCTATCAAGATCAATTG+CGG - Chr2:25584843-25584862 None:intergenic 35.0%
TACTATCTCTTCTACCTTCA+AGG + Chr2:25583110-25583129 MsG0280008110.01.T01:intron 35.0%
TCAACAAGATGTCTATCAGT+TGG - Chr2:25584509-25584528 None:intergenic 35.0%
TCACCAAACAATACTAAAGG+GGG + Chr2:25583452-25583471 MsG0280008110.01.T01:intron 35.0%
TTAGGGCTTGAATTATTACC+AGG + Chr2:25582570-25582589 MsG0280008110.01.T01:CDS 35.0%
TTCCAGCTAGGAAAAAATTG+AGG - Chr2:25583059-25583078 None:intergenic 35.0%
TTCGTAACATGAACTTGAAC+AGG - Chr2:25585070-25585089 None:intergenic 35.0%
TTTCATGCACACAAATGAAG+GGG - Chr2:25581955-25581974 None:intergenic 35.0%
TTTCTCAAGTTTACCCTTGT+TGG - Chr2:25584199-25584218 None:intergenic 35.0%
! AGCTCTTGACACAAGTTTTA+AGG + Chr2:25584058-25584077 MsG0280008110.01.T01:intron 35.0%
! AGTCGGTTCAAAATTGTTAG+AGG - Chr2:25583431-25583450 None:intergenic 35.0%
! ATGCTTTTGTTGCATTTTGG+AGG - Chr2:25584556-25584575 None:intergenic 35.0%
! CCCCCATCTAATTTTACAAT+TGG - Chr2:25585202-25585221 None:intergenic 35.0%
! GGAATTTTGTTGAAGTTAGC+AGG + Chr2:25584983-25585002 MsG0280008110.01.T01:CDS 35.0%
! GTTCAAGTTCATGTTACGAA+TGG + Chr2:25585070-25585089 MsG0280008110.01.T01:CDS 35.0%
! GTTTATGGTGTTTTCCAACA+AGG + Chr2:25584182-25584201 MsG0280008110.01.T01:intron 35.0%
! TACCGATGTTGCAATTTTCT+CGG + Chr2:25584874-25584893 MsG0280008110.01.T01:CDS 35.0%
! TAGTATTGTTTGGTGAGAGT+CGG - Chr2:25583448-25583467 None:intergenic 35.0%
! TCATTCTCTCTATGTGGTTT+TGG + Chr2:25582422-25582441 MsG0280008110.01.T01:intron 35.0%
! TGCTTTTGTTGCATTTTGGA+GGG - Chr2:25584555-25584574 None:intergenic 35.0%
! TGGATGCTTTTGTTGCATTT+TGG - Chr2:25584559-25584578 None:intergenic 35.0%
! TTATCTGTTTTGCACTGCTA+AGG - Chr2:25583826-25583845 None:intergenic 35.0%
! TTCATTTTCCAACAAGGCTT+TGG - Chr2:25583506-25583525 None:intergenic 35.0%
!! ATTGTCGATTCTGATTCTAG+CGG - Chr2:25584690-25584709 None:intergenic 35.0%
!!! AACCTCAATTTTTTCCTAGC+TGG + Chr2:25583054-25583073 MsG0280008110.01.T01:intron 35.0%
!!! AATGGTTTTTTCTTAGGGGA+TGG + Chr2:25585088-25585107 MsG0280008110.01.T01:CDS 35.0%
!!! AGCATTTCTCTTGTCACTTT+TGG + Chr2:25582042-25582061 MsG0280008110.01.T01:CDS 35.0%
!!! CAGATCTCCTTTATTTTGTC+TGG - Chr2:25583655-25583674 None:intergenic 35.0%
AAGGTTCCTCCATTTACCTT+TGG - Chr2:25584467-25584486 None:intergenic 40.0%
AATGAAGAAGAGTGGAAGAG+TGG + Chr2:25582452-25582471 MsG0280008110.01.T01:intron 40.0%
CAATGACCAAAGGTAAATGG+AGG + Chr2:25584458-25584477 MsG0280008110.01.T01:intron 40.0%
CAGCCATTGGAATTGTTCTT+AGG + Chr2:25584897-25584916 MsG0280008110.01.T01:CDS 40.0%
CTCACTGGACACACATATTT+AGG - Chr2:25583333-25583352 None:intergenic 40.0%
GAAAGTGAGAAACCAGTTCA+TGG + Chr2:25585460-25585479 MsG0280008110.01.T01:CDS 40.0%
GCCATTGGAATTGTTCTTAG+GGG + Chr2:25584899-25584918 MsG0280008110.01.T01:CDS 40.0%
GGATCTGCACCATACTTAAT+TGG - Chr2:25582483-25582502 None:intergenic 40.0%
GTGGTGTACCCAATTAAGTA+TGG + Chr2:25582471-25582490 MsG0280008110.01.T01:CDS 40.0%
TAGCCCCCTTTAGTATTGTT+TGG - Chr2:25583458-25583477 None:intergenic 40.0%
TCCCATCACATTTCCTTCAT+CGG + Chr2:25582653-25582672 MsG0280008110.01.T01:CDS 40.0%
TCCCCTAAGAACAATTCCAA+TGG - Chr2:25584903-25584922 None:intergenic 40.0%
TGCCGAGAAAATTGCAACAT+CGG - Chr2:25584879-25584898 None:intergenic 40.0%
TTACCAGGAATTAGAGACCT+TGG + Chr2:25582585-25582604 MsG0280008110.01.T01:CDS 40.0%
TTCATGCACACAAATGAAGG+GGG - Chr2:25581954-25581973 None:intergenic 40.0%
TTCCTCCATTTCAACACAGA+AGG + Chr2:25584716-25584735 MsG0280008110.01.T01:CDS 40.0%
TTCCTTCTGTGTTGAAATGG+AGG - Chr2:25584721-25584740 None:intergenic 40.0%
TTCTACGTGTTCATTGAAGC+AGG - Chr2:25582092-25582111 None:intergenic 40.0%
! ATGAGTTTGAGATCAACGGT+TGG + Chr2:25585271-25585290 MsG0280008110.01.T01:CDS 40.0%
! TGAGAAATCCAAAGCCTTGT+TGG + Chr2:25583495-25583514 MsG0280008110.01.T01:intron 40.0%
! TGAGTTTGAGATCAACGGTT+GGG + Chr2:25585272-25585291 MsG0280008110.01.T01:CDS 40.0%
! TGGCATGAGTTTGAGATCAA+CGG + Chr2:25585267-25585286 MsG0280008110.01.T01:CDS 40.0%
!! AACAAAGCAACAGGTTTTGG+TGG + Chr2:25584956-25584975 MsG0280008110.01.T01:CDS 40.0%
!! ACAAGGCTTTGGATTTCTCA+AGG - Chr2:25583495-25583514 None:intergenic 40.0%
!! GGATGGTAATGGCACAAAAT+GGG + Chr2:25585306-25585325 MsG0280008110.01.T01:CDS 40.0%
!! TGGATGGTAATGGCACAAAA+TGG + Chr2:25585305-25585324 MsG0280008110.01.T01:CDS 40.0%
!! TGTTGCATTTTGGAGGGTTT+TGG - Chr2:25584549-25584568 None:intergenic 40.0%
!!! CTCTCTATGTGGTTTTGGAT+TGG + Chr2:25582427-25582446 MsG0280008110.01.T01:intron 40.0%
AACGGTTGGGAAGTTGAATG+TGG + Chr2:25585285-25585304 MsG0280008110.01.T01:CDS 45.0%
AAGTATGGTGCAGATCCAAC+AGG + Chr2:25582486-25582505 MsG0280008110.01.T01:CDS 45.0%
AGCCGATGAAGGAAATGTGA+TGG - Chr2:25582658-25582677 None:intergenic 45.0%
AGTATGGTGCAGATCCAACA+GGG + Chr2:25582487-25582506 MsG0280008110.01.T01:CDS 45.0%
ATCACATTTCCTTCATCGGC+TGG + Chr2:25582657-25582676 MsG0280008110.01.T01:CDS 45.0%
CGATGAATATTCCTCCACCT+CGG - Chr2:25584661-25584680 None:intergenic 45.0%
CTCGGTAGCTTGAATCGAAA+AGG - Chr2:25584643-25584662 None:intergenic 45.0%
CTGTAGTATCAACCTCTTCG+AGG - Chr2:25583086-25583105 None:intergenic 45.0%
CTTGGACAACACTCAACAGT+CGG + Chr2:25584785-25584804 MsG0280008110.01.T01:CDS 45.0%
GCCGATGAAGGAAATGTGAT+GGG - Chr2:25582657-25582676 None:intergenic 45.0%
GGACAAGCTAACATGATCAC+AGG + Chr2:25584920-25584939 MsG0280008110.01.T01:CDS 45.0%
GTGTTTCCTAGTACGCTTCT+AGG - Chr2:25583362-25583381 None:intergenic 45.0%
GTTGGGAAGTTGAATGTGGA+TGG + Chr2:25585289-25585308 MsG0280008110.01.T01:CDS 45.0%
TACGTGTTCATTGAAGCAGG+TGG - Chr2:25582089-25582108 None:intergenic 45.0%
! CTGCTTTCATTTTCCCCTGT+TGG - Chr2:25582504-25582523 None:intergenic 45.0%
! CTTTTCGATTCAAGCTACCG+AGG + Chr2:25584641-25584660 MsG0280008110.01.T01:CDS 45.0%
! GGTAATGGCACAAAATGGGT+GGG + Chr2:25585310-25585329 MsG0280008110.01.T01:CDS 45.0%
!! GCAACAGGTTTTGGTGGAAT+TGG + Chr2:25584962-25584981 MsG0280008110.01.T01:CDS 45.0%
!! TGGTAATGGCACAAAATGGG+TGG + Chr2:25585309-25585328 MsG0280008110.01.T01:CDS 45.0%
!! AATTTAAATAAAACTTTATT+TGG - Chr2:25583162-25583181 None:intergenic 5.0%
!!! TTTTTATAATATTTATTAAG+AGG + Chr2:25583188-25583207 MsG0280008110.01.T01:intron 5.0%
ACAAGATTGCCAGCCGATGA+AGG - Chr2:25582669-25582688 None:intergenic 50.0%
AGCTGGAAAACACCTCGAAG+AGG + Chr2:25583071-25583090 MsG0280008110.01.T01:intron 50.0%
CCAGGAATTAGAGACCTTGG+AGG + Chr2:25582588-25582607 MsG0280008110.01.T01:CDS 50.0%
CCTAGTACGCTTCTAGGTAC+AGG - Chr2:25583356-25583375 None:intergenic 50.0%
CCTCCAAGGTCTCTAATTCC+TGG - Chr2:25582591-25582610 None:intergenic 50.0%
GTATGGTGCAGATCCAACAG+GGG + Chr2:25582488-25582507 MsG0280008110.01.T01:CDS 50.0%
GTGTGAAGATGGCTGAGACA+TGG - Chr2:25582161-25582180 None:intergenic 50.0%
TTCGATTCAAGCTACCGAGG+TGG + Chr2:25584644-25584663 MsG0280008110.01.T01:CDS 50.0%
! CCTGTACCTAGAAGCGTACT+AGG + Chr2:25583353-25583372 MsG0280008110.01.T01:intron 50.0%
! GCAATTTTCTCGGCAGCCAT+TGG + Chr2:25584884-25584903 MsG0280008110.01.T01:CDS 50.0%
! TTCATCGGCTGGCAATCTTG+TGG + Chr2:25582668-25582687 MsG0280008110.01.T01:CDS 50.0%
!! CTTCGAGGTGTTTTCCAGCT+AGG - Chr2:25583071-25583090 None:intergenic 50.0%
GATTCAAGCTACCGAGGTGG+AGG + Chr2:25584647-25584666 MsG0280008110.01.T01:CDS 55.0%
GCTTCTAGGTACAGGCTCAC+TGG - Chr2:25583348-25583367 None:intergenic 55.0%
GGACAACACTCAACAGTCGG+TGG + Chr2:25584788-25584807 MsG0280008110.01.T01:CDS 55.0%
Chromosome Type Strat End Strand Name
Chr2 gene 25581929 25585535 25581929 ID=MsG0280008110.01;Name=MsG0280008110.01
Chr2 mRNA 25581929 25585535 25581929 ID=MsG0280008110.01.T01;Parent=MsG0280008110.01;Name=MsG0280008110.01.T01;_AED=0.40;_eAED=0.40;_QI=87|1|1|1|0.5|0.33|3|25|481
Chr2 exon 25581929 25582189 25581929 ID=MsG0280008110.01.T01:exon:14060;Parent=MsG0280008110.01.T01
Chr2 exon 25582459 25582689 25582459 ID=MsG0280008110.01.T01:exon:14061;Parent=MsG0280008110.01.T01
Chr2 exon 25584470 25585535 25584470 ID=MsG0280008110.01.T01:exon:14062;Parent=MsG0280008110.01.T01
Chr2 five_prime_UTR 25581929 25582015 25581929 ID=MsG0280008110.01.T01:five_prime_utr;Parent=MsG0280008110.01.T01
Chr2 CDS 25582016 25582189 25582016 ID=MsG0280008110.01.T01:cds;Parent=MsG0280008110.01.T01
Chr2 CDS 25582459 25582689 25582459 ID=MsG0280008110.01.T01:cds;Parent=MsG0280008110.01.T01
Chr2 CDS 25584470 25585510 25584470 ID=MsG0280008110.01.T01:cds;Parent=MsG0280008110.01.T01
Chr2 three_prime_UTR 25585511 25585535 25585511 ID=MsG0280008110.01.T01:three_prime_utr;Parent=MsG0280008110.01.T01
Gene Sequence

>MsG0280008110.01.T01

ATGTTTGCTATGAGATTCTCAACCTTAGCATTTCTCTTGTCACTTTTGGTGATACTACAAGTACAAGAAACCACCTGCTTCAATGAACACGTAGAACTTTCTCATCTTGTGAAAAAAATTGTTAAGCAAAAAATGGGCATTGCCATGTCTCAGCCATCTTCACACAAAAAAAAGAAGAGTGGAAGAGTGGTGTACCCAATTAAGTATGGTGCAGATCCAACAGGGGAAAATGAAAGCAGTGATTCCATTATGAAAGCTGTAGAAGCTGCATTTGATATTGATAATTTAGGGCTTGAATTATTACCAGGAATTAGAGACCTTGGAGGTGTTATCATTGATTTTCAAGGTGGAAACTATAAAATCAGCAATCCCATCACATTTCCTTCATCGGCTGGCAATCTTGTGGTAAATGGAGGAACCTTAAGAGCTTCTGAAACATTTCCAACTGATAGACATCTTGTTGAATTAAATGCATCAAATTCCAAAACCCTCCAAAATGCAACAAAAGCATCCAACAACAGATTACTTGAACAAACGATAGGAATTTACTATGAAGACATAACATTTAGAGACATCCTTTTCGATTCAAGCTACCGAGGTGGAGGAATATTCATCGTCGATTCCGCTAGAATCAGAATCGACAATTGTTTCTTCCTCCATTTCAACACAGAAGGAATTAAAGTTCAAAGTGGCCATGAAACCTTCATATCAAGTAGTTTTCTTGGACAACACTCAACAGTCGGTGGAGACAAAGACGAAAGACAATTCTCAGGAACCGCAATTGATCTTGATAGCAACGACAATGCAGTTACCGATGTTGCAATTTTCTCGGCAGCCATTGGAATTGTTCTTAGGGGACAAGCTAACATGATCACAGGTGTGCATTGTTATAACAAAGCAACAGGTTTTGGTGGAATTGGAATTTTGTTGAAGTTAGCAGGGAATTCACAAACAAGAATTGATAATTGTTACATGGATTATAATTCTATAGTCATGGAAGATCCTGTTCAAGTTCATGTTACGAATGGTTTTTTCTTAGGGGATGGTAATATTGTTTTGAAGTCAATTAAAGGGAAAGTTTATGGTTTAAATATTGTTAACAATATGTTTAGTGGAAACCCTAATAATAATGTTCCAATTGTAAAATTAGATGGGGGATTTAGTAACATTGATCAAGTGGTAATTGATATGAACAATGTGGTTGGCATGAGTTTGAGATCAACGGTTGGGAAGTTGAATGTGGATGGTAATGGCACAAAATGGGTGGGTGATTTTTCAAATGTTTTGGTGTTTCCTAATAGGATTAGTCATTTTCAGTATTCATTTCATACATTAGAGGGACCAAAGTTTGTTGCACATTCAGTGAGTAATGTGTCAAATAATGCTTTAATTGTTGAAAGTGAGAAACCAGTTCATGGTGTTGTTTCTTTCTTTGTTGAACAATGA
Protein sequence

>MsG0280008110.01.T01

MFAMRFSTLAFLLSLLVILQVQETTCFNEHVELSHLVKKIVKQKMGIAMSQPSSHKKKKSGRVVYPIKYGADPTGENESSDSIMKAVEAAFDIDNLGLELLPGIRDLGGVIIDFQGGNYKISNPITFPSSAGNLVVNGGTLRASETFPTDRHLVELNASNSKTLQNATKASNNRLLEQTIGIYYEDITFRDILFDSSYRGGGIFIVDSARIRIDNCFFLHFNTEGIKVQSGHETFISSSFLGQHSTVGGDKDERQFSGTAIDLDSNDNAVTDVAIFSAAIGIVLRGQANMITGVHCYNKATGFGGIGILLKLAGNSQTRIDNCYMDYNSIVMEDPVQVHVTNGFFLGDGNIVLKSIKGKVYGLNIVNNMFSGNPNNNVPIVKLDGGFSNIDQVVIDMNNVVGMSLRSTVGKLNVDGNGTKWVGDFSNVLVFPNRISHFQYSFHTLEGPKFVAHSVSNVSNNALIVESEKPVHGVVSFFVEQ*