Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MsG0280008282.01.T01 | ABN08693.1 | 68.852 | 122 | 38 | 0 | 21 | 142 | 3 | 124 | 2.66E-50 | 168 |
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MsG0280008282.01.T01 | A2Q4Z3 | 68.852 | 122 | 38 | 0 | 21 | 142 | 3 | 124 | 1.27e-50 | 168 |
| Gene ID | Type | Classification |
|---|
| Gene ID | Type | Classification |
|---|
Co-expression Network
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|
PPI
| Gene1 | Gene2 | Type |
|---|---|---|
| MsG0280009322.01 | MsG0280008282.01 | PPI |
| MsG0780041116.01 | MsG0280008282.01 | PPI |
| MsG0780040274.01 | MsG0280008282.01 | PPI |
| MsG0280010449.01 | MsG0280008282.01 | PPI |
| MsG0780041116.01 | MsG0280008282.01 | PPI |
| MsG0380013578.01 | MsG0280008282.01 | PPI |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MsG0280008282.01.T01 | MTR_5g075610 | 62.626 | 99 | 35 | 1 | 26 | 124 | 1 | 97 | 4.73e-36 | 121 |
| MsG0280008282.01.T01 | MTR_1g088650 | 53.922 | 102 | 47 | 0 | 22 | 123 | 88 | 189 | 1.23e-31 | 112 |
| MsG0280008282.01.T01 | MTR_4g008270 | 54.762 | 84 | 38 | 0 | 20 | 103 | 10 | 93 | 1.08e-28 | 102 |
| MsG0280008282.01.T01 | MTR_1g034430 | 50.588 | 85 | 42 | 0 | 22 | 106 | 97 | 181 | 1.19e-26 | 99.8 |
| MsG0280008282.01.T01 | MTR_2g059470 | 54.878 | 82 | 37 | 0 | 22 | 103 | 2 | 83 | 2.86e-26 | 95.9 |
| MsG0280008282.01.T01 | MTR_7g063280 | 40.196 | 102 | 61 | 0 | 21 | 122 | 73 | 174 | 1.68e-25 | 96.7 |
| MsG0280008282.01.T01 | MTR_8g031800 | 54.667 | 75 | 34 | 0 | 21 | 95 | 32 | 106 | 6.72e-25 | 93.2 |
| MsG0280008282.01.T01 | MTR_7g082130 | 40.000 | 120 | 48 | 2 | 16 | 134 | 39 | 135 | 1.41e-22 | 87.8 |
| MsG0280008282.01.T01 | MTR_5g085210 | 35.659 | 129 | 67 | 1 | 6 | 134 | 105 | 217 | 1.26e-21 | 87.8 |
| MsG0280008282.01.T01 | MTR_6g016265 | 47.059 | 85 | 45 | 0 | 22 | 106 | 41 | 125 | 1.66e-21 | 84.7 |
| MsG0280008282.01.T01 | MTR_5g049680 | 51.316 | 76 | 36 | 1 | 21 | 95 | 32 | 107 | 4.09e-21 | 83.2 |
| MsG0280008282.01.T01 | MTR_8g098925 | 44.681 | 94 | 52 | 0 | 24 | 117 | 69 | 162 | 1.10e-19 | 81.3 |
| MsG0280008282.01.T01 | MTR_8g022750 | 44.554 | 101 | 39 | 1 | 18 | 118 | 203 | 286 | 1.51e-19 | 83.6 |
| MsG0280008282.01.T01 | MTR_1g045560 | 37.963 | 108 | 51 | 2 | 26 | 133 | 1 | 92 | 1.55e-19 | 79.0 |
| MsG0280008282.01.T01 | MTR_2g027020 | 36.667 | 120 | 72 | 1 | 20 | 139 | 28 | 143 | 2.15e-19 | 80.9 |
| MsG0280008282.01.T01 | MTR_6g089560 | 42.222 | 90 | 52 | 0 | 19 | 108 | 115 | 204 | 2.67e-19 | 81.6 |
| MsG0280008282.01.T01 | MTR_0093s0070 | 53.425 | 73 | 34 | 0 | 23 | 95 | 126 | 198 | 3.07e-19 | 80.9 |
| MsG0280008282.01.T01 | MTR_1g074090 | 50.746 | 67 | 33 | 0 | 19 | 85 | 80 | 146 | 4.57e-19 | 79.3 |
| MsG0280008282.01.T01 | MTR_1g017840 | 48.750 | 80 | 41 | 0 | 24 | 103 | 82 | 161 | 1.46e-18 | 78.2 |
| MsG0280008282.01.T01 | MTR_3g116210 | 39.604 | 101 | 57 | 1 | 4 | 104 | 85 | 181 | 1.88e-18 | 78.6 |
| MsG0280008282.01.T01 | MTR_0015s0120 | 48.611 | 72 | 37 | 0 | 20 | 91 | 293 | 364 | 5.84e-18 | 80.1 |
| MsG0280008282.01.T01 | MTR_1g074240 | 52.381 | 63 | 30 | 0 | 22 | 84 | 2 | 64 | 6.54e-18 | 73.9 |
| MsG0280008282.01.T01 | MTR_8g079540 | 49.315 | 73 | 37 | 0 | 19 | 91 | 47 | 119 | 1.19e-17 | 74.7 |
| MsG0280008282.01.T01 | MTR_1g069475 | 43.750 | 80 | 45 | 0 | 22 | 101 | 166 | 245 | 1.50e-17 | 77.4 |
| MsG0280008282.01.T01 | MTR_7g053400 | 55.172 | 58 | 26 | 0 | 24 | 81 | 179 | 236 | 2.51e-17 | 77.0 |
| MsG0280008282.01.T01 | MTR_6g453270 | 39.773 | 88 | 53 | 0 | 21 | 108 | 301 | 388 | 3.13e-17 | 78.2 |
| MsG0280008282.01.T01 | MTR_6g034915 | 35.593 | 118 | 58 | 2 | 16 | 133 | 52 | 151 | 7.25e-17 | 74.3 |
| MsG0280008282.01.T01 | MTR_5g037920 | 39.655 | 116 | 68 | 2 | 24 | 138 | 102 | 216 | 7.97e-17 | 75.1 |
| MsG0280008282.01.T01 | MTR_8g059135 | 39.773 | 88 | 53 | 0 | 23 | 110 | 31 | 118 | 2.06e-16 | 71.6 |
| MsG0280008282.01.T01 | MTR_5g036320 | 51.515 | 66 | 32 | 0 | 20 | 85 | 79 | 144 | 7.44e-16 | 70.9 |
| MsG0280008282.01.T01 | MTR_7g013520 | 48.000 | 75 | 39 | 0 | 21 | 95 | 71 | 145 | 1.30e-15 | 70.9 |
| MsG0280008282.01.T01 | MTR_1g081930 | 40.909 | 88 | 52 | 0 | 21 | 108 | 39 | 126 | 5.75e-15 | 68.2 |
| MsG0280008282.01.T01 | MTR_5g083200 | 43.038 | 79 | 45 | 0 | 22 | 100 | 100 | 178 | 8.20e-15 | 69.3 |
| MsG0280008282.01.T01 | MTR_0002s1220 | 43.038 | 79 | 45 | 0 | 22 | 100 | 100 | 178 | 8.20e-15 | 69.3 |
| MsG0280008282.01.T01 | MTR_8g099580 | 43.529 | 85 | 48 | 0 | 19 | 103 | 205 | 289 | 8.39e-15 | 70.9 |
| MsG0280008282.01.T01 | MTR_5g034450 | 48.438 | 64 | 33 | 0 | 20 | 83 | 49 | 112 | 1.09e-14 | 67.0 |
| MsG0280008282.01.T01 | MTR_8g036780 | 50.847 | 59 | 29 | 0 | 20 | 78 | 34 | 92 | 1.19e-14 | 66.6 |
| MsG0280008282.01.T01 | MTR_1g050488 | 45.161 | 62 | 34 | 0 | 22 | 83 | 100 | 161 | 1.93e-14 | 67.4 |
| MsG0280008282.01.T01 | MTR_3g111390 | 41.772 | 79 | 46 | 0 | 22 | 100 | 100 | 178 | 2.95e-14 | 67.8 |
| MsG0280008282.01.T01 | MTR_1g070820 | 41.772 | 79 | 46 | 0 | 22 | 100 | 100 | 178 | 2.95e-14 | 67.8 |
| MsG0280008282.01.T01 | MTR_5g091440 | 32.773 | 119 | 47 | 2 | 26 | 144 | 1 | 86 | 3.99e-14 | 65.9 |
| MsG0280008282.01.T01 | MTR_6g016135 | 48.333 | 60 | 31 | 0 | 24 | 83 | 383 | 442 | 5.03e-14 | 68.9 |
| MsG0280008282.01.T01 | MTR_1g045340 | 41.772 | 79 | 46 | 0 | 22 | 100 | 86 | 164 | 5.98e-14 | 66.6 |
| MsG0280008282.01.T01 | MTR_8g088220 | 42.308 | 78 | 44 | 1 | 26 | 103 | 1 | 77 | 7.62e-14 | 63.5 |
| MsG0280008282.01.T01 | MTR_5g069700 | 43.939 | 66 | 37 | 0 | 42 | 107 | 3 | 68 | 1.37e-13 | 63.2 |
| MsG0280008282.01.T01 | MTR_1g057740 | 47.059 | 68 | 36 | 0 | 75 | 142 | 58 | 125 | 2.05e-13 | 64.3 |
| MsG0280008282.01.T01 | MTR_8g028785 | 38.710 | 93 | 57 | 0 | 33 | 125 | 271 | 363 | 9.80e-13 | 65.1 |
| MsG0280008282.01.T01 | MTR_4g029360 | 35.294 | 102 | 66 | 0 | 19 | 120 | 126 | 227 | 1.31e-12 | 63.9 |
| MsG0280008282.01.T01 | MTR_6g016355 | 44.262 | 61 | 34 | 0 | 21 | 81 | 117 | 177 | 3.98e-12 | 61.6 |
| MsG0280008282.01.T01 | MTR_8g038340 | 46.667 | 60 | 32 | 0 | 18 | 77 | 187 | 246 | 5.13e-12 | 62.4 |
| MsG0280008282.01.T01 | MTR_4g094370 | 57.447 | 47 | 20 | 0 | 16 | 62 | 31 | 77 | 5.17e-12 | 58.9 |
| MsG0280008282.01.T01 | MTR_1g062840 | 43.333 | 60 | 34 | 0 | 42 | 101 | 2 | 61 | 5.45e-12 | 59.3 |
| MsG0280008282.01.T01 | MTR_1g099430 | 41.935 | 62 | 34 | 1 | 21 | 80 | 126 | 187 | 6.57e-12 | 61.6 |
| MsG0280008282.01.T01 | MTR_7g103930 | 43.056 | 72 | 31 | 1 | 61 | 132 | 10 | 71 | 7.32e-12 | 60.1 |
| MsG0280008282.01.T01 | MTR_3g026270 | 43.636 | 55 | 31 | 0 | 26 | 80 | 1 | 55 | 9.74e-12 | 57.8 |
| MsG0280008282.01.T01 | MTR_2g055490 | 48.387 | 62 | 32 | 0 | 25 | 86 | 12 | 73 | 2.08e-11 | 58.2 |
| MsG0280008282.01.T01 | MTR_7g072030 | 46.000 | 50 | 27 | 0 | 42 | 91 | 71 | 120 | 2.57e-11 | 58.2 |
| MsG0280008282.01.T01 | MTR_2g047100 | 37.374 | 99 | 47 | 1 | 21 | 119 | 19 | 102 | 3.69e-11 | 57.4 |
| MsG0280008282.01.T01 | MTR_6g013970 | 37.333 | 75 | 47 | 0 | 28 | 102 | 107 | 181 | 6.99e-11 | 58.9 |
| MsG0280008282.01.T01 | MTR_1g017820 | 50.000 | 46 | 23 | 0 | 38 | 83 | 18 | 63 | 8.86e-11 | 55.5 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|
Find 25 sgRNAs with CRISPR-Local
Find 34 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| TTTGTATTACTCAAAGTTTC+AGG | 0.219087 | 2:+28592889 | None:intergenic |
| TAAAGCATCCACAACCTTCT+TGG | 0.243809 | 2:+28593137 | None:intergenic |
| TCTTTCATGTTGAGTTTAAT+AGG | 0.250158 | 2:-28593035 | MsG0280008282.01.T01:CDS |
| GGTTATGAGCAGGTGGGAAT+TGG | 0.309384 | 2:-28593318 | MsG0280008282.01.T01:CDS |
| CCTATGTCAACATCGCAAAT+AGG | 0.398606 | 2:+28593237 | None:intergenic |
| GTTATGAGCAGGTGGGAATT+GGG | 0.444231 | 2:-28593317 | MsG0280008282.01.T01:CDS |
| GTCGATTATGTTACGATGAC+TGG | 0.454800 | 2:-28593384 | None:intergenic |
| CCTATTTGCGATGTTGACAT+AGG | 0.457666 | 2:-28593237 | MsG0280008282.01.T01:CDS |
| CATCACTCGTAAGTTAGTTC+AGG | 0.460900 | 2:-28592998 | MsG0280008282.01.T01:CDS |
| CTATTTGCGATGTTGACATA+GGG | 0.473976 | 2:-28593236 | MsG0280008282.01.T01:CDS |
| TTGTTCTTACAAAAGCATCT+TGG | 0.487711 | 2:-28593266 | MsG0280008282.01.T01:CDS |
| GCGTCGATGTTACACTTCAA+TGG | 0.514088 | 2:-28593353 | MsG0280008282.01.T01:CDS |
| TCTAGACTCCAAGAAGGTTG+TGG | 0.526815 | 2:-28593145 | MsG0280008282.01.T01:CDS |
| TTAAGAACATGTGTAGAAGA+TGG | 0.554185 | 2:-28593117 | MsG0280008282.01.T01:CDS |
| TTTCTTCGGTTATGAGCAGG+TGG | 0.578085 | 2:-28593325 | MsG0280008282.01.T01:CDS |
| TTCATGTTGAGTTTAATAGG+AGG | 0.596908 | 2:-28593032 | MsG0280008282.01.T01:CDS |
| TTGTGAGAGCTAGCATTACG+TGG | 0.612840 | 2:+28592970 | None:intergenic |
| TGTGAGAGCTAGCATTACGT+GGG | 0.641952 | 2:+28592971 | None:intergenic |
| CAAAATCACATGCGTCATCG+CGG | 0.644228 | 2:+28593286 | None:intergenic |
| TTCTTCGGTTATGAGCAGGT+GGG | 0.645605 | 2:-28593324 | MsG0280008282.01.T01:CDS |
| GTAACCCACCTAAAATAGTG+TGG | 0.675169 | 2:-28592924 | MsG0280008282.01.T01:CDS |
| TATTTGCGATGTTGACATAG+GGG | 0.679975 | 2:-28593235 | MsG0280008282.01.T01:CDS |
| ACCCACCTAAAATAGTGTGG+TGG | 0.687799 | 2:-28592921 | MsG0280008282.01.T01:CDS |
| GTGAGAGCTAGCATTACGTG+GGG | 0.709819 | 2:+28592972 | None:intergenic |
| ATTGAAGTGTAACATCGACG+CGG | 0.800289 | 2:+28593355 | None:intergenic |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|---|---|---|---|---|
| !!! | ACAATTATTTTTTTGGAAAA+AGG | - | Chr2:28593300-28593319 | MsG0280008282.01.T01:CDS | 15.0% |
| ! | TCTTTCATGTTGAGTTTAAT+AGG | - | Chr2:28593216-28593235 | MsG0280008282.01.T01:CDS | 25.0% |
| ! | TTTGTATTACTCAAAGTTTC+AGG | + | Chr2:28593365-28593384 | None:intergenic | 25.0% |
| !!! | AGCTCTCACAATTATTTTTT+TGG | - | Chr2:28593293-28593312 | MsG0280008282.01.T01:CDS | 25.0% |
| TTAAGAACATGTGTAGAAGA+TGG | - | Chr2:28593134-28593153 | MsG0280008282.01.T01:CDS | 30.0% | |
| TTCATGTTGAGTTTAATAGG+AGG | - | Chr2:28593219-28593238 | MsG0280008282.01.T01:CDS | 30.0% | |
| !! | TTGTTCTTACAAAAGCATCT+TGG | - | Chr2:28592985-28593004 | MsG0280008282.01.T01:CDS | 30.0% |
| !!! | AGTTGTTTTTCTTACAGCTT+TGG | - | Chr2:28593043-28593062 | MsG0280008282.01.T01:CDS | 30.0% |
| CTATTTGCGATGTTGACATA+GGG | - | Chr2:28593015-28593034 | MsG0280008282.01.T01:CDS | 35.0% | |
| TATTTGCGATGTTGACATAG+GGG | - | Chr2:28593016-28593035 | MsG0280008282.01.T01:CDS | 35.0% | |
| ! | ATTCTCCACCACACTATTTT+AGG | + | Chr2:28593338-28593357 | None:intergenic | 35.0% |
| ! | CTTCAATGGCTCATTTTCTT+CGG | - | Chr2:28592912-28592931 | MsG0280008282.01.T01:CDS | 35.0% |
| ! | TTTTACTCTAGACTCCAAGA+AGG | - | Chr2:28593100-28593119 | MsG0280008282.01.T01:CDS | 35.0% |
| !!! | TTTTTCTTACAGCTTTGGAG+TGG | - | Chr2:28593048-28593067 | MsG0280008282.01.T01:CDS | 35.0% |
| ATTGAAGTGTAACATCGACG+CGG | + | Chr2:28592899-28592918 | None:intergenic | 40.0% | |
| CATCACTCGTAAGTTAGTTC+AGG | - | Chr2:28593253-28593272 | MsG0280008282.01.T01:CDS | 40.0% | |
| CCTATGTCAACATCGCAAAT+AGG | + | Chr2:28593017-28593036 | None:intergenic | 40.0% | |
| CCTATTTGCGATGTTGACAT+AGG | - | Chr2:28593014-28593033 | MsG0280008282.01.T01:CDS | 40.0% | |
| GTAACCCACCTAAAATAGTG+TGG | - | Chr2:28593327-28593346 | MsG0280008282.01.T01:CDS | 40.0% | |
| ! | CATTTTCTTCGGTTATGAGC+AGG | - | Chr2:28592923-28592942 | MsG0280008282.01.T01:CDS | 40.0% |
| ! | TAAAGCATCCACAACCTTCT+TGG | + | Chr2:28593117-28593136 | None:intergenic | 40.0% |
| !! | TCCACCACACTATTTTAGGT+GGG | + | Chr2:28593334-28593353 | None:intergenic | 40.0% |
| ACCCACCTAAAATAGTGTGG+TGG | - | Chr2:28593330-28593349 | MsG0280008282.01.T01:CDS | 45.0% | |
| CAAAATCACATGCGTCATCG+CGG | + | Chr2:28592968-28592987 | None:intergenic | 45.0% | |
| GTTATGAGCAGGTGGGAATT+GGG | - | Chr2:28592934-28592953 | MsG0280008282.01.T01:CDS | 45.0% | |
| TCTAGACTCCAAGAAGGTTG+TGG | - | Chr2:28593106-28593125 | MsG0280008282.01.T01:CDS | 45.0% | |
| TGTGAGAGCTAGCATTACGT+GGG | + | Chr2:28593283-28593302 | None:intergenic | 45.0% | |
| TTCTTCGGTTATGAGCAGGT+GGG | - | Chr2:28592927-28592946 | MsG0280008282.01.T01:CDS | 45.0% | |
| TTGTGAGAGCTAGCATTACG+TGG | + | Chr2:28593284-28593303 | None:intergenic | 45.0% | |
| TTTCTTCGGTTATGAGCAGG+TGG | - | Chr2:28592926-28592945 | MsG0280008282.01.T01:CDS | 45.0% | |
| ! | GCGTCGATGTTACACTTCAA+TGG | - | Chr2:28592898-28592917 | MsG0280008282.01.T01:CDS | 45.0% |
| !! | CTCCACCACACTATTTTAGG+TGG | + | Chr2:28593335-28593354 | None:intergenic | 45.0% |
| GGTTATGAGCAGGTGGGAAT+TGG | - | Chr2:28592933-28592952 | MsG0280008282.01.T01:CDS | 50.0% | |
| GTGAGAGCTAGCATTACGTG+GGG | + | Chr2:28593282-28593301 | None:intergenic | 50.0% |
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| Chr2 | gene | 28592882 | 28593391 | 28592882 | ID=MsG0280008282.01;Name=MsG0280008282.01 |
| Chr2 | mRNA | 28592882 | 28593391 | 28592882 | ID=MsG0280008282.01.T01;Parent=MsG0280008282.01;Name=MsG0280008282.01.T01;_AED=0.46;_eAED=0.46;_QI=0|-1|0|1|-1|1|1|0|169 |
| Chr2 | exon | 28592882 | 28593391 | 28592882 | ID=MsG0280008282.01.T01:exon:14409;Parent=MsG0280008282.01.T01 |
| Chr2 | CDS | 28592882 | 28593391 | 28592882 | ID=MsG0280008282.01.T01:cds;Parent=MsG0280008282.01.T01 |
| Gene Sequence |
| Protein sequence |
