Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MsG0280008311.01.T01 | ABN08745.1 | 83.333 | 222 | 35 | 1 | 1 | 220 | 1 | 222 | 4.67E-119 | 347 |
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MsG0280008311.01.T01 | A2Q545 | 83.333 | 222 | 35 | 1 | 1 | 220 | 1 | 222 | 2.23e-119 | 347 |
| Gene ID | Type | Classification |
|---|
| Gene ID | Type | Classification |
|---|
Co-expression Network
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|---|---|---|---|
| MsG0280008311.01 | MsG0580025338.01 | 0.807997 | 3.845101e-50 | 2.173166e-47 |
| MsG0280008311.01 | MsG0580028560.01 | 0.801324 | 9.500961e-49 | 4.524260e-46 |
PPI
| Gene1 | Gene2 | Type |
|---|
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MsG0280008311.01.T01 | MTR_2g039320 | 83.333 | 222 | 35 | 1 | 1 | 220 | 1 | 222 | 5.66e-123 | 347 |
| MsG0280008311.01.T01 | MTR_4g126210 | 36.475 | 244 | 108 | 8 | 12 | 215 | 7 | 243 | 5.40e-31 | 114 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|
Find 55 sgRNAs with CRISPR-Local
Find 66 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| CGTTCATCGTCGTCGTTTCT+TGG | 0.238445 | 2:+29085106 | MsG0280008311.01.T01:CDS |
| TTGAGTTCCAGTTCTGATTC+AGG | 0.253071 | 2:-29085436 | None:intergenic |
| CTCTTGCAACGCCTGATGTC+GGG | 0.305557 | 2:+29085364 | MsG0280008311.01.T01:CDS |
| TTAATCGTTAACCTTGTCAT+AGG | 0.350745 | 2:+29085013 | MsG0280008311.01.T01:CDS |
| GACGATGAACGGACAAGTTC+CGG | 0.363566 | 2:-29085094 | None:intergenic |
| TGTTCGACCTGGGTTTCTTC+CGG | 0.407677 | 2:-29085196 | None:intergenic |
| AAGACTTGACCCGGGTCAAC+AGG | 0.410251 | 2:+29085230 | MsG0280008311.01.T01:CDS |
| GTTGTTGCTGCTGCTGATGC+TGG | 0.414720 | 2:-29085169 | None:intergenic |
| GACAACATCGTCTACTTTGT+TGG | 0.414991 | 2:+29085252 | MsG0280008311.01.T01:CDS |
| TTCGGTAAAGGTGTTGGAGG+AGG | 0.422401 | 2:+29085405 | MsG0280008311.01.T01:CDS |
| GACGAAAATTCCCCGACATC+AGG | 0.426254 | 2:-29085375 | None:intergenic |
| ATCAAAGGTGTAGAATCAAC+TGG | 0.427211 | 2:-29085328 | None:intergenic |
| AAAGGTTTAAGCAGCAACTT+AGG | 0.427984 | 2:+29085530 | MsG0280008311.01.T01:CDS |
| TGTTGACCCGGGTCAAGTCT+TGG | 0.440866 | 2:-29085228 | None:intergenic |
| TTTACAGCAATTGAGTCTAA+AGG | 0.445981 | 2:-29085140 | None:intergenic |
| GTTTAAGCAGCAACTTAGGT+TGG | 0.453539 | 2:+29085534 | MsG0280008311.01.T01:CDS |
| TATATGATATAATTGTAAGT+TGG | 0.457863 | 2:+29084969 | MsG0280008311.01.T01:CDS |
| CATCGTCTACTTTGTTGGAC+CGG | 0.457887 | 2:+29085257 | MsG0280008311.01.T01:CDS |
| GGAAGAAACCCAGGTCGAAC+AGG | 0.460194 | 2:+29085198 | MsG0280008311.01.T01:CDS |
| TGTGAGAGGAAGGGCAGAAG+AGG | 0.462732 | 2:+29085474 | MsG0280008311.01.T01:CDS |
| CCTCTTGCAACGCCTGATGT+CGG | 0.464245 | 2:+29085363 | MsG0280008311.01.T01:CDS |
| TCGGTAAAGGTGTTGGAGGA+GGG | 0.464553 | 2:+29085406 | MsG0280008311.01.T01:CDS |
| GAAGAAACCTGAATCAGAAC+TGG | 0.465681 | 2:+29085429 | MsG0280008311.01.T01:CDS |
| ACGATGTTGTCCTGTTGACC+CGG | 0.483259 | 2:-29085240 | None:intergenic |
| AAAGAGAGTTTGTGAGAGGA+AGG | 0.500435 | 2:+29085464 | MsG0280008311.01.T01:CDS |
| AGATTCGGTAAAGGTGTTGG+AGG | 0.508799 | 2:+29085402 | MsG0280008311.01.T01:CDS |
| CTCAGTAAAACAGAAACTAA+TGG | 0.514548 | 2:+29085301 | MsG0280008311.01.T01:CDS |
| CCGGGTCAAGTCAATCGACC+TGG | 0.519795 | 2:+29085276 | MsG0280008311.01.T01:CDS |
| ATTAGATTCGGTAAAGGTGT+TGG | 0.521171 | 2:+29085399 | MsG0280008311.01.T01:CDS |
| CGGTAAAGGTGTTGGAGGAG+GGG | 0.523953 | 2:+29085407 | MsG0280008311.01.T01:CDS |
| AGCAGCAACTTAGGTTGGAA+AGG | 0.527408 | 2:+29085539 | MsG0280008311.01.T01:CDS |
| TCGTCGATTAGATTCGGTAA+AGG | 0.534224 | 2:+29085393 | MsG0280008311.01.T01:CDS |
| AGGGACGGAGCTCGGATCAA+AGG | 0.540543 | 2:-29085343 | None:intergenic |
| GTTGCAAGAGGGACGGAGCT+CGG | 0.541927 | 2:-29085351 | None:intergenic |
| AGAGGAAGAAGTTGATGCAA+AGG | 0.542473 | 2:+29085492 | MsG0280008311.01.T01:CDS |
| AGCAGCAGCAACAACAACGC+CGG | 0.546513 | 2:+29085177 | MsG0280008311.01.T01:CDS |
| ATCGTCTACTTTGTTGGACC+GGG | 0.548993 | 2:+29085258 | MsG0280008311.01.T01:CDS |
| TCTTGCAACGCCTGATGTCG+GGG | 0.551303 | 2:+29085365 | MsG0280008311.01.T01:CDS |
| CCAGGTCGATTGACTTGACC+CGG | 0.552190 | 2:-29085276 | None:intergenic |
| CAAGAAACGACGACGATGAA+CGG | 0.560499 | 2:-29085105 | None:intergenic |
| AAAATGAGAAGTAATAGCTA+TGG | 0.577116 | 2:-29085038 | None:intergenic |
| CGACATCAGGCGTTGCAAGA+GGG | 0.586588 | 2:-29085362 | None:intergenic |
| AACAACGCCGGAAGAAACCC+AGG | 0.587857 | 2:+29085189 | MsG0280008311.01.T01:CDS |
| CCGACATCAGGCGTTGCAAG+AGG | 0.595808 | 2:-29085363 | None:intergenic |
| CGGGTCAAGTCAATCGACCT+GGG | 0.600972 | 2:+29085277 | MsG0280008311.01.T01:CDS |
| AAGAGAGTTTGTGAGAGGAA+GGG | 0.607363 | 2:+29085465 | MsG0280008311.01.T01:CDS |
| ACGGCAACTCGACTCACCGC+CGG | 0.608052 | 2:+29085075 | MsG0280008311.01.T01:CDS |
| CGATGTTGTCCTGTTGACCC+GGG | 0.610417 | 2:-29085239 | None:intergenic |
| ATCAGGCGTTGCAAGAGGGA+CGG | 0.611532 | 2:-29085358 | None:intergenic |
| CCTAAAAGAGAGTTTGTGAG+AGG | 0.612664 | 2:+29085460 | MsG0280008311.01.T01:CDS |
| TCAAAGGTGTAGAATCAACT+GGG | 0.665485 | 2:-29085327 | None:intergenic |
| TAGCTATGGTACCTATGACA+AGG | 0.682585 | 2:-29085024 | None:intergenic |
| AGTAAAACAGAAACTAATGG+AGG | 0.692399 | 2:+29085304 | MsG0280008311.01.T01:CDS |
| ACGATTAAGAATATACACGA+AGG | 0.720504 | 2:-29084998 | None:intergenic |
| GATGAACGGACAAGTTCCGG+CGG | 0.731375 | 2:-29085091 | None:intergenic |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|---|---|---|---|---|
| !! | TATATGATATAATTGTAAGT+TGG | + | Chr2:29084969-29084988 | MsG0280008311.01.T01:CDS | 15.0% |
| !!! | ATTTTGCCACTAAAAAAAAA+CGG | + | Chr2:29085056-29085075 | MsG0280008311.01.T01:CDS | 20.0% |
| ! | AAAATGAGAAGTAATAGCTA+TGG | - | Chr2:29085041-29085060 | None:intergenic | 25.0% |
| !! | AGGCTGATGATTTTATAAAA+AGG | + | Chr2:29085512-29085531 | MsG0280008311.01.T01:CDS | 25.0% |
| ACGATTAAGAATATACACGA+AGG | - | Chr2:29085001-29085020 | None:intergenic | 30.0% | |
| AGTAAAACAGAAACTAATGG+AGG | + | Chr2:29085304-29085323 | MsG0280008311.01.T01:CDS | 30.0% | |
| CTCAGTAAAACAGAAACTAA+TGG | + | Chr2:29085301-29085320 | MsG0280008311.01.T01:CDS | 30.0% | |
| TTAATCGTTAACCTTGTCAT+AGG | + | Chr2:29085013-29085032 | MsG0280008311.01.T01:CDS | 30.0% | |
| TTTACAGCAATTGAGTCTAA+AGG | - | Chr2:29085143-29085162 | None:intergenic | 30.0% | |
| !! | CATTAGTTTCTGTTTTACTG+AGG | - | Chr2:29085303-29085322 | None:intergenic | 30.0% |
| !! | GAATTTTCGTCGATTAGATT+CGG | + | Chr2:29085387-29085406 | MsG0280008311.01.T01:CDS | 30.0% |
| AAAGGTTTAAGCAGCAACTT+AGG | + | Chr2:29085530-29085549 | MsG0280008311.01.T01:CDS | 35.0% | |
| TTAAAAACCAAGACTTGACC+CGG | + | Chr2:29085221-29085240 | MsG0280008311.01.T01:CDS | 35.0% | |
| ! | ATCAAAGGTGTAGAATCAAC+TGG | - | Chr2:29085331-29085350 | None:intergenic | 35.0% |
| ! | ATTAGATTCGGTAAAGGTGT+TGG | + | Chr2:29085399-29085418 | MsG0280008311.01.T01:CDS | 35.0% |
| ! | TCAAAGGTGTAGAATCAACT+GGG | - | Chr2:29085330-29085349 | None:intergenic | 35.0% |
| !!! | GAGTTGCCGTTTTTTTTTAG+TGG | - | Chr2:29085065-29085084 | None:intergenic | 35.0% |
| AAAGAGAGTTTGTGAGAGGA+AGG | + | Chr2:29085464-29085483 | MsG0280008311.01.T01:CDS | 40.0% | |
| AAGAGAGTTTGTGAGAGGAA+GGG | + | Chr2:29085465-29085484 | MsG0280008311.01.T01:CDS | 40.0% | |
| CCTAAAAGAGAGTTTGTGAG+AGG | + | Chr2:29085460-29085479 | MsG0280008311.01.T01:CDS | 40.0% | |
| GAAGAAACCTGAATCAGAAC+TGG | + | Chr2:29085429-29085448 | MsG0280008311.01.T01:CDS | 40.0% | |
| GACAACATCGTCTACTTTGT+TGG | + | Chr2:29085252-29085271 | MsG0280008311.01.T01:CDS | 40.0% | |
| GTTTAAGCAGCAACTTAGGT+TGG | + | Chr2:29085534-29085553 | MsG0280008311.01.T01:CDS | 40.0% | |
| TAAAAACCAAGACTTGACCC+GGG | + | Chr2:29085222-29085241 | MsG0280008311.01.T01:CDS | 40.0% | |
| TAGCTATGGTACCTATGACA+AGG | - | Chr2:29085027-29085046 | None:intergenic | 40.0% | |
| ! | CCTCTCACAAACTCTCTTTT+AGG | - | Chr2:29085463-29085482 | None:intergenic | 40.0% |
| ! | GTTTTTAACCTGTTCGACCT+GGG | - | Chr2:29085209-29085228 | None:intergenic | 40.0% |
| ! | TCGTCGATTAGATTCGGTAA+AGG | + | Chr2:29085393-29085412 | MsG0280008311.01.T01:CDS | 40.0% |
| ! | TTGAGTTCCAGTTCTGATTC+AGG | - | Chr2:29085439-29085458 | None:intergenic | 40.0% |
| !! | AGAGGAAGAAGTTGATGCAA+AGG | + | Chr2:29085492-29085511 | MsG0280008311.01.T01:CDS | 40.0% |
| ATCGTCTACTTTGTTGGACC+GGG | + | Chr2:29085258-29085277 | MsG0280008311.01.T01:CDS | 45.0% | |
| CAAGAAACGACGACGATGAA+CGG | - | Chr2:29085108-29085127 | None:intergenic | 45.0% | |
| CATCGTCTACTTTGTTGGAC+CGG | + | Chr2:29085257-29085276 | MsG0280008311.01.T01:CDS | 45.0% | |
| ! | GGTTTTTAACCTGTTCGACC+TGG | - | Chr2:29085210-29085229 | None:intergenic | 45.0% |
| ! | TTTCTGTTTTACTGAGGCCC+AGG | - | Chr2:29085297-29085316 | None:intergenic | 45.0% |
| !! | AGATTCGGTAAAGGTGTTGG+AGG | + | Chr2:29085402-29085421 | MsG0280008311.01.T01:CDS | 45.0% |
| !! | AGCAGCAACTTAGGTTGGAA+AGG | + | Chr2:29085539-29085558 | MsG0280008311.01.T01:CDS | 45.0% |
| ACGATGTTGTCCTGTTGACC+CGG | - | Chr2:29085243-29085262 | None:intergenic | 50.0% | |
| CGTTCATCGTCGTCGTTTCT+TGG | + | Chr2:29085106-29085125 | MsG0280008311.01.T01:CDS | 50.0% | |
| GACGAAAATTCCCCGACATC+AGG | - | Chr2:29085378-29085397 | None:intergenic | 50.0% | |
| GACGATGAACGGACAAGTTC+CGG | - | Chr2:29085097-29085116 | None:intergenic | 50.0% | |
| TGTTCGACCTGGGTTTCTTC+CGG | - | Chr2:29085199-29085218 | None:intergenic | 50.0% | |
| !! | TCGGTAAAGGTGTTGGAGGA+GGG | + | Chr2:29085406-29085425 | MsG0280008311.01.T01:CDS | 50.0% |
| !! | TTCGGTAAAGGTGTTGGAGG+AGG | + | Chr2:29085405-29085424 | MsG0280008311.01.T01:CDS | 50.0% |
| AACAACGCCGGAAGAAACCC+AGG | + | Chr2:29085189-29085208 | MsG0280008311.01.T01:CDS | 55.0% | |
| AAGACTTGACCCGGGTCAAC+AGG | + | Chr2:29085230-29085249 | MsG0280008311.01.T01:CDS | 55.0% | |
| AGCAGCAGCAACAACAACGC+CGG | + | Chr2:29085177-29085196 | MsG0280008311.01.T01:CDS | 55.0% | |
| CCTCTTGCAACGCCTGATGT+CGG | + | Chr2:29085363-29085382 | MsG0280008311.01.T01:CDS | 55.0% | |
| CGATGTTGTCCTGTTGACCC+GGG | - | Chr2:29085242-29085261 | None:intergenic | 55.0% | |
| CGGGTCAAGTCAATCGACCT+GGG | + | Chr2:29085277-29085296 | MsG0280008311.01.T01:CDS | 55.0% | |
| CTCTTGCAACGCCTGATGTC+GGG | + | Chr2:29085364-29085383 | MsG0280008311.01.T01:CDS | 55.0% | |
| GATGAACGGACAAGTTCCGG+CGG | - | Chr2:29085094-29085113 | None:intergenic | 55.0% | |
| GGAAGAAACCCAGGTCGAAC+AGG | + | Chr2:29085198-29085217 | MsG0280008311.01.T01:CDS | 55.0% | |
| GTTGTTGCTGCTGCTGATGC+TGG | - | Chr2:29085172-29085191 | None:intergenic | 55.0% | |
| TCTTGCAACGCCTGATGTCG+GGG | + | Chr2:29085365-29085384 | MsG0280008311.01.T01:CDS | 55.0% | |
| TGTGAGAGGAAGGGCAGAAG+AGG | + | Chr2:29085474-29085493 | MsG0280008311.01.T01:CDS | 55.0% | |
| TGTTGACCCGGGTCAAGTCT+TGG | - | Chr2:29085231-29085250 | None:intergenic | 55.0% | |
| ! | ATCAGGCGTTGCAAGAGGGA+CGG | - | Chr2:29085361-29085380 | None:intergenic | 55.0% |
| ! | CGACATCAGGCGTTGCAAGA+GGG | - | Chr2:29085365-29085384 | None:intergenic | 55.0% |
| !! | CCAGGTCGATTGACTTGACC+CGG | - | Chr2:29085279-29085298 | None:intergenic | 55.0% |
| !! | CGGTAAAGGTGTTGGAGGAG+GGG | + | Chr2:29085407-29085426 | MsG0280008311.01.T01:CDS | 55.0% |
| AGGGACGGAGCTCGGATCAA+AGG | - | Chr2:29085346-29085365 | None:intergenic | 60.0% | |
| CCGGGTCAAGTCAATCGACC+TGG | + | Chr2:29085276-29085295 | MsG0280008311.01.T01:CDS | 60.0% | |
| GTTGCAAGAGGGACGGAGCT+CGG | - | Chr2:29085354-29085373 | None:intergenic | 60.0% | |
| ! | CCGACATCAGGCGTTGCAAG+AGG | - | Chr2:29085366-29085385 | None:intergenic | 60.0% |
| ACGGCAACTCGACTCACCGC+CGG | + | Chr2:29085075-29085094 | MsG0280008311.01.T01:CDS | 65.0% |
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| Chr2 | gene | 29084944 | 29085606 | 29084944 | ID=MsG0280008311.01;Name=MsG0280008311.01 |
| Chr2 | mRNA | 29084944 | 29085606 | 29084944 | ID=MsG0280008311.01.T01;Parent=MsG0280008311.01;Name=MsG0280008311.01.T01;_AED=0.50;_eAED=0.50;_QI=0|-1|0|1|-1|1|1|0|220 |
| Chr2 | exon | 29084944 | 29085606 | 29084944 | ID=MsG0280008311.01.T01:exon:5111;Parent=MsG0280008311.01.T01 |
| Chr2 | CDS | 29084944 | 29085606 | 29084944 | ID=MsG0280008311.01.T01:cds;Parent=MsG0280008311.01.T01 |
| Gene Sequence |
| Protein sequence |
