Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MsG0280008387.01.T01 | KEH37569.1 | 88.739 | 222 | 18 | 2 | 1 | 222 | 1 | 215 | 1.87E-132 | 387 |
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MsG0280008387.01.T01 | Q3EBZ2 | 28.755 | 233 | 138 | 7 | 5 | 223 | 2 | 220 | 4.73E-18 | 85.1 |
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MsG0280008387.01.T01 | A0A072V678 | 88.739 | 222 | 18 | 2 | 1 | 222 | 1 | 215 | 8.95e-133 | 387 |
| Gene ID | Type | Classification |
|---|
| Gene ID | Type | Classification |
|---|
Co-expression Network
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|---|---|---|---|
| MsG0280008387.01 | MsG0880046440.01 | 0.804896 | 1.733688e-49 | 9.046138e-47 |
PPI
| Gene1 | Gene2 | Type |
|---|
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MsG0280008387.01.T01 | MTR_2g041510 | 88.739 | 222 | 18 | 2 | 1 | 222 | 1 | 215 | 2.27e-136 | 387 |
| MsG0280008387.01.T01 | MTR_2g041510 | 88.739 | 222 | 18 | 2 | 1 | 222 | 21 | 235 | 3.58e-136 | 388 |
| MsG0280008387.01.T01 | MTR_8g074610 | 78.924 | 223 | 46 | 1 | 1 | 222 | 20 | 242 | 5.83e-126 | 362 |
| MsG0280008387.01.T01 | MTR_8g074640 | 71.300 | 223 | 63 | 1 | 1 | 222 | 1 | 223 | 2.45e-108 | 317 |
| MsG0280008387.01.T01 | MTR_6g092570 | 34.199 | 231 | 118 | 8 | 2 | 222 | 4 | 210 | 3.51e-25 | 101 |
| MsG0280008387.01.T01 | MTR_3g030050 | 31.304 | 230 | 138 | 6 | 5 | 223 | 15 | 235 | 1.49e-22 | 95.1 |
| MsG0280008387.01.T01 | MTR_8g085760 | 30.242 | 248 | 130 | 10 | 2 | 227 | 9 | 235 | 1.18e-21 | 92.4 |
| MsG0280008387.01.T01 | MTR_5g079830 | 29.918 | 244 | 130 | 8 | 1 | 228 | 1 | 219 | 1.78e-21 | 92.0 |
| MsG0280008387.01.T01 | MTR_7g096720 | 30.544 | 239 | 126 | 10 | 8 | 227 | 15 | 232 | 2.14e-16 | 77.4 |
| MsG0280008387.01.T01 | MTR_1g026850 | 29.876 | 241 | 121 | 11 | 1 | 222 | 1 | 212 | 8.56e-15 | 72.8 |
| MsG0280008387.01.T01 | MTR_1g007260 | 26.471 | 238 | 138 | 9 | 5 | 223 | 21 | 240 | 1.29e-14 | 72.4 |
| MsG0280008387.01.T01 | MTR_6g018390 | 28.936 | 235 | 134 | 9 | 5 | 222 | 4 | 222 | 3.10e-14 | 71.2 |
| MsG0280008387.01.T01 | MTR_2g047180 | 27.273 | 231 | 144 | 8 | 3 | 222 | 1 | 218 | 7.08e-14 | 70.1 |
| MsG0280008387.01.T01 | MTR_3g029650 | 27.160 | 243 | 120 | 9 | 5 | 225 | 4 | 211 | 2.51e-13 | 68.9 |
| MsG0280008387.01.T01 | MTR_3g029650 | 25.311 | 241 | 140 | 9 | 4 | 221 | 370 | 593 | 5.48e-13 | 68.2 |
| MsG0280008387.01.T01 | MTR_6g018360 | 26.531 | 245 | 127 | 8 | 5 | 222 | 4 | 222 | 4.41e-13 | 68.2 |
| MsG0280008387.01.T01 | MTR_1g016570 | 28.947 | 228 | 127 | 8 | 6 | 223 | 21 | 223 | 6.19e-13 | 67.4 |
| MsG0280008387.01.T01 | MTR_3g114970 | 28.378 | 222 | 124 | 9 | 5 | 220 | 3 | 195 | 1.10e-12 | 66.6 |
| MsG0280008387.01.T01 | MTR_6g027100 | 27.016 | 248 | 130 | 10 | 5 | 222 | 4 | 230 | 1.14e-12 | 67.0 |
| MsG0280008387.01.T01 | MTR_3g029640 | 27.755 | 245 | 128 | 8 | 3 | 224 | 1 | 219 | 1.48e-12 | 66.2 |
| MsG0280008387.01.T01 | MTR_6g018400 | 26.556 | 241 | 138 | 9 | 3 | 222 | 2 | 224 | 2.81e-12 | 65.5 |
| MsG0280008387.01.T01 | MTR_3g029620 | 26.778 | 239 | 140 | 7 | 5 | 222 | 2 | 226 | 3.97e-12 | 65.1 |
| MsG0280008387.01.T01 | MTR_1g049170 | 31.897 | 116 | 65 | 3 | 1 | 115 | 1 | 103 | 1.71e-11 | 63.2 |
| MsG0280008387.01.T01 | MTR_3g058460 | 30.531 | 226 | 125 | 11 | 6 | 222 | 15 | 217 | 3.64e-11 | 62.4 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MsG0280008387.01.T01 | AT2G17030 | 28.755 | 233 | 138 | 7 | 5 | 223 | 2 | 220 | 4.82e-19 | 85.1 |
| MsG0280008387.01.T01 | AT4G35733 | 25.103 | 243 | 124 | 7 | 1 | 222 | 1 | 206 | 1.09e-12 | 66.6 |
| MsG0280008387.01.T01 | AT3G25750 | 25.311 | 241 | 125 | 9 | 3 | 222 | 2 | 208 | 1.22e-11 | 63.5 |
| MsG0280008387.01.T01 | AT2G17036 | 25.620 | 242 | 138 | 10 | 5 | 225 | 1 | 221 | 3.58e-11 | 62.4 |
| MsG0280008387.01.T01 | AT2G17036 | 25.620 | 242 | 138 | 10 | 5 | 225 | 2 | 222 | 3.58e-11 | 62.4 |
| MsG0280008387.01.T01 | AT1G65770 | 26.522 | 230 | 135 | 9 | 5 | 223 | 2 | 208 | 8.86e-11 | 61.2 |
Find 43 sgRNAs with CRISPR-Local
Find 49 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| TATAAAATTGAAAAGATTAA+AGG | 0.167135 | 2:-30529966 | MsG0280008387.01.T01:CDS |
| TGATAATGGGGAACTATTTC+AGG | 0.173559 | 2:-30529745 | MsG0280008387.01.T01:CDS |
| AAAGGAAAAGAAGGAGAATT+TGG | 0.264948 | 2:+30530269 | None:intergenic |
| TATGGAACATGTTGGATTAT+AGG | 0.282617 | 2:-30530010 | MsG0280008387.01.T01:CDS |
| AGAAGAAGAAGGTGCTAAAC+TGG | 0.284874 | 2:+30530157 | None:intergenic |
| ATGGAACATGTTGGATTATA+GGG | 0.306390 | 2:-30530009 | MsG0280008387.01.T01:CDS |
| AGTTGGTTGAATAAGATAAA+TGG | 0.329748 | 2:+30530196 | None:intergenic |
| ATAACACTTCCCTGATGATA+TGG | 0.343400 | 2:-30530028 | MsG0280008387.01.T01:CDS |
| CTTCTTCTTCTTCCAAAGGT+TGG | 0.349032 | 2:-30530139 | MsG0280008387.01.T01:CDS |
| TAAAACATGGTGATGAGAAA+TGG | 0.387502 | 2:-30529836 | MsG0280008387.01.T01:CDS |
| ACACTACTCCAAGGAGAGTT+AGG | 0.387864 | 2:+30529909 | None:intergenic |
| TAGCTTAATCAACCAACCTT+TGG | 0.393448 | 2:+30530127 | None:intergenic |
| CTCAACTCCCTACAGAACTA+TGG | 0.406799 | 2:-30530385 | MsG0280008387.01.T01:CDS |
| GAATAAGATAAATGGTTGAT+TGG | 0.414572 | 2:+30530204 | None:intergenic |
| TGTTCTATCCTAACTCTCCT+TGG | 0.425110 | 2:-30529917 | MsG0280008387.01.T01:CDS |
| TGACGATATTATTGTGTTCA+AGG | 0.429582 | 2:-30529784 | MsG0280008387.01.T01:CDS |
| CCAACATGTTCCATATCATC+AGG | 0.435629 | 2:+30530018 | None:intergenic |
| TTGGTTGAGGTAGTTGTTTG+TGG | 0.440994 | 2:+30530223 | None:intergenic |
| GAAGGAGAATTTGGAAGAGA+AGG | 0.441388 | 2:+30530278 | None:intergenic |
| ACAGAACTATGGCCTAAGAT+AGG | 0.442853 | 2:-30530374 | MsG0280008387.01.T01:CDS |
| GATAAATGGTTGATTGGTTG+AGG | 0.469906 | 2:+30530210 | None:intergenic |
| ATCTTTCAAGAAGATATAGT+AGG | 0.478424 | 2:-30529864 | MsG0280008387.01.T01:CDS |
| AATTATCATATCCTCTTAGC+AGG | 0.479331 | 2:-30530052 | MsG0280008387.01.T01:CDS |
| ATTTGGAAGAGAAGGAGGAA+TGG | 0.482176 | 2:+30530286 | None:intergenic |
| ATCTTAGGCCATAGTTCTGT+AGG | 0.489468 | 2:+30530377 | None:intergenic |
| AGAAGGTGCTAAACTGGAGT+TGG | 0.502318 | 2:+30530163 | None:intergenic |
| GGAGAATTTGGAAGAGAAGG+AGG | 0.519680 | 2:+30530281 | None:intergenic |
| GGTATTTGCAAAGGAAAAGA+AGG | 0.529929 | 2:+30530260 | None:intergenic |
| TAGAACACAACTTTCTTCAC+AGG | 0.530312 | 2:+30529933 | None:intergenic |
| TTTGTGGAGATGTTGATGCC+AGG | 0.530799 | 2:+30530239 | None:intergenic |
| CCAATCTACTTTCTGATCCA+TGG | 0.537119 | 2:+30530409 | None:intergenic |
| CCATGGATCAGAAAGTAGAT+TGG | 0.542655 | 2:-30530409 | MsG0280008387.01.T01:CDS |
| CTCTCCTTGGAGTAGTGTTG+AGG | 0.544030 | 2:-30529904 | MsG0280008387.01.T01:CDS |
| GACGATATTATTGTGTTCAA+GGG | 0.544331 | 2:-30529783 | MsG0280008387.01.T01:CDS |
| CCTGATGATATGGAACATGT+TGG | 0.555978 | 2:-30530018 | MsG0280008387.01.T01:CDS |
| TCTTAGGCCATAGTTCTGTA+GGG | 0.578657 | 2:+30530378 | None:intergenic |
| GAAGTGTTATTCCTGCTAAG+AGG | 0.592416 | 2:+30530041 | None:intergenic |
| ATAGGATTCAACAAACTCAA+AGG | 0.597227 | 2:+30530086 | None:intergenic |
| TTGATGCCAGGTATTTGCAA+AGG | 0.610176 | 2:+30530251 | None:intergenic |
| GAGTTGGAACTTGCATCAGT+TGG | 0.641493 | 2:+30530179 | None:intergenic |
| CAACATGTTCCATATCATCA+GGG | 0.642106 | 2:+30530019 | None:intergenic |
| CAATCCTCAACACTACTCCA+AGG | 0.642424 | 2:+30529900 | None:intergenic |
| ATAGTAGGATTCATAAAACA+TGG | 0.671857 | 2:-30529849 | MsG0280008387.01.T01:CDS |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|---|---|---|---|---|
| !! | TATAAAATTGAAAAGATTAA+AGG | - | Chr2:30530181-30530200 | MsG0280008387.01.T01:CDS | 10.0% |
| !!! | AGTTTTATGTTACTGATAAT+GGG | - | Chr2:30530389-30530408 | MsG0280008387.01.T01:CDS | 20.0% |
| ! | AGTTGGTTGAATAAGATAAA+TGG | + | Chr2:30529954-30529973 | None:intergenic | 25.0% |
| ! | ATAGTAGGATTCATAAAACA+TGG | - | Chr2:30530298-30530317 | MsG0280008387.01.T01:CDS | 25.0% |
| ! | ATCTTTCAAGAAGATATAGT+AGG | - | Chr2:30530283-30530302 | MsG0280008387.01.T01:CDS | 25.0% |
| ! | GAATAAGATAAATGGTTGAT+TGG | + | Chr2:30529946-30529965 | None:intergenic | 25.0% |
| !! | GATAATTTTCTTTCAGTGAT+AGG | + | Chr2:30530082-30530101 | None:intergenic | 25.0% |
| !! | TCAAGATATTTTCCTATCTT+AGG | + | Chr2:30529788-30529807 | None:intergenic | 25.0% |
| !!! | CAGTTTTATGTTACTGATAA+TGG | - | Chr2:30530388-30530407 | MsG0280008387.01.T01:CDS | 25.0% |
| !!! | GTTTTATGTTACTGATAATG+GGG | - | Chr2:30530390-30530409 | MsG0280008387.01.T01:CDS | 25.0% |
| AAAGGAAAAGAAGGAGAATT+TGG | + | Chr2:30529881-30529900 | None:intergenic | 30.0% | |
| AATTATCATATCCTCTTAGC+AGG | - | Chr2:30530095-30530114 | MsG0280008387.01.T01:CDS | 30.0% | |
| ATAGGATTCAACAAACTCAA+AGG | + | Chr2:30530064-30530083 | None:intergenic | 30.0% | |
| ATGGAACATGTTGGATTATA+GGG | - | Chr2:30530138-30530157 | MsG0280008387.01.T01:CDS | 30.0% | |
| GACGATATTATTGTGTTCAA+GGG | - | Chr2:30530364-30530383 | MsG0280008387.01.T01:CDS | 30.0% | |
| TATGGAACATGTTGGATTAT+AGG | - | Chr2:30530137-30530156 | MsG0280008387.01.T01:CDS | 30.0% | |
| TGACGATATTATTGTGTTCA+AGG | - | Chr2:30530363-30530382 | MsG0280008387.01.T01:CDS | 30.0% | |
| ! | TAAAACATGGTGATGAGAAA+TGG | - | Chr2:30530311-30530330 | MsG0280008387.01.T01:CDS | 30.0% |
| ATAACACTTCCCTGATGATA+TGG | - | Chr2:30530119-30530138 | MsG0280008387.01.T01:CDS | 35.0% | |
| CAACATGTTCCATATCATCA+GGG | + | Chr2:30530131-30530150 | None:intergenic | 35.0% | |
| GGTATTTGCAAAGGAAAAGA+AGG | + | Chr2:30529890-30529909 | None:intergenic | 35.0% | |
| TAGAACACAACTTTCTTCAC+AGG | + | Chr2:30530217-30530236 | None:intergenic | 35.0% | |
| TAGCTTAATCAACCAACCTT+TGG | + | Chr2:30530023-30530042 | None:intergenic | 35.0% | |
| TCTTCTTCTTCTTCTTCCAA+AGG | - | Chr2:30530004-30530023 | MsG0280008387.01.T01:CDS | 35.0% | |
| TTGGAAGAAGAAGAAGAAGA+AGG | + | Chr2:30530004-30530023 | None:intergenic | 35.0% | |
| ! | GATAAATGGTTGATTGGTTG+AGG | + | Chr2:30529940-30529959 | None:intergenic | 35.0% |
| ! | TCTTTTCCTTTGCAAATACC+TGG | - | Chr2:30529890-30529909 | MsG0280008387.01.T01:CDS | 35.0% |
| !! | TGATAATGGGGAACTATTTC+AGG | - | Chr2:30530402-30530421 | MsG0280008387.01.T01:CDS | 35.0% |
| ACAGAACTATGGCCTAAGAT+AGG | - | Chr2:30529773-30529792 | MsG0280008387.01.T01:CDS | 40.0% | |
| ATTTGGAAGAGAAGGAGGAA+TGG | + | Chr2:30529864-30529883 | None:intergenic | 40.0% | |
| CCAACATGTTCCATATCATC+AGG | + | Chr2:30530132-30530151 | None:intergenic | 40.0% | |
| CCTGATGATATGGAACATGT+TGG | - | Chr2:30530129-30530148 | MsG0280008387.01.T01:CDS | 40.0% | |
| CTTCTTCTTCTTCCAAAGGT+TGG | - | Chr2:30530008-30530027 | MsG0280008387.01.T01:CDS | 40.0% | |
| GAAGGAGAATTTGGAAGAGA+AGG | + | Chr2:30529872-30529891 | None:intergenic | 40.0% | |
| TGTTCTATCCTAACTCTCCT+TGG | - | Chr2:30530230-30530249 | MsG0280008387.01.T01:CDS | 40.0% | |
| TTGATGCCAGGTATTTGCAA+AGG | + | Chr2:30529899-30529918 | None:intergenic | 40.0% | |
| TTGGTTGAGGTAGTTGTTTG+TGG | + | Chr2:30529927-30529946 | None:intergenic | 40.0% | |
| ! | ATCTTAGGCCATAGTTCTGT+AGG | + | Chr2:30529773-30529792 | None:intergenic | 40.0% |
| ! | GAAGTGTTATTCCTGCTAAG+AGG | + | Chr2:30530109-30530128 | None:intergenic | 40.0% |
| ! | TCTTAGGCCATAGTTCTGTA+GGG | + | Chr2:30529772-30529791 | None:intergenic | 40.0% |
| !! | AGAAGAAGAAGGTGCTAAAC+TGG | + | Chr2:30529993-30530012 | None:intergenic | 40.0% |
| ACACTACTCCAAGGAGAGTT+AGG | + | Chr2:30530241-30530260 | None:intergenic | 45.0% | |
| CAATCCTCAACACTACTCCA+AGG | + | Chr2:30530250-30530269 | None:intergenic | 45.0% | |
| CTCAACTCCCTACAGAACTA+TGG | - | Chr2:30529762-30529781 | MsG0280008387.01.T01:CDS | 45.0% | |
| GAGTTGGAACTTGCATCAGT+TGG | + | Chr2:30529971-30529990 | None:intergenic | 45.0% | |
| GGAGAATTTGGAAGAGAAGG+AGG | + | Chr2:30529869-30529888 | None:intergenic | 45.0% | |
| TTTGTGGAGATGTTGATGCC+AGG | + | Chr2:30529911-30529930 | None:intergenic | 45.0% | |
| !! | AGAAGGTGCTAAACTGGAGT+TGG | + | Chr2:30529987-30530006 | None:intergenic | 45.0% |
| CTCTCCTTGGAGTAGTGTTG+AGG | - | Chr2:30530243-30530262 | MsG0280008387.01.T01:CDS | 50.0% |
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| Chr2 | gene | 30529740 | 30530429 | 30529740 | ID=MsG0280008387.01;Name=MsG0280008387.01 |
| Chr2 | mRNA | 30529740 | 30530429 | 30529740 | ID=MsG0280008387.01.T01;Parent=MsG0280008387.01;Name=MsG0280008387.01.T01;_AED=0.50;_eAED=0.50;_QI=0|-1|0|1|-1|1|1|0|229 |
| Chr2 | exon | 30529740 | 30530429 | 30529740 | ID=MsG0280008387.01.T01:exon:5910;Parent=MsG0280008387.01.T01 |
| Chr2 | CDS | 30529740 | 30530429 | 30529740 | ID=MsG0280008387.01.T01:cds;Parent=MsG0280008387.01.T01 |
| Gene Sequence |
| Protein sequence |
