Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MsG0880046440.01.T01 | GAU17167.1 | 62.022 | 445 | 73 | 6 | 1 | 356 | 2 | 439 | 0 | 544 |
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MsG0880046440.01.T01 | A0A2Z6LMV2 | 62.022 | 445 | 73 | 6 | 1 | 356 | 2 | 439 | 0.0 | 544 |
Co-expression Network
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|---|---|---|---|
| MsG0080048177.01 | MsG0880046440.01 | 0.807520 | 4.856636e-50 | 2.710875e-47 |
| MsG0080048178.01 | MsG0880046440.01 | 0.808478 | 3.037216e-50 | 1.738462e-47 |
| MsG0180000065.01 | MsG0880046440.01 | 0.804212 | 2.406989e-49 | 1.233847e-46 |
| MsG0180000267.01 | MsG0880046440.01 | 0.829172 | 5.971841e-55 | 6.064866e-52 |
| MsG0180000585.01 | MsG0880046440.01 | 0.811662 | 6.257912e-51 | 3.896451e-48 |
| MsG0180000673.01 | MsG0880046440.01 | 0.801631 | 8.218442e-49 | 3.943940e-46 |
| MsG0180000822.01 | MsG0880046440.01 | 0.807868 | 4.096533e-50 | 2.307296e-47 |
| MsG0180002273.01 | MsG0880046440.01 | 0.800479 | 1.413516e-48 | 6.588462e-46 |
| MsG0180002894.01 | MsG0880046440.01 | 0.815973 | 7.023958e-52 | 4.914221e-49 |
| MsG0180002952.01 | MsG0880046440.01 | 0.807229 | 5.596130e-50 | 3.100136e-47 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MsG0880046440.01.T01 | MTR_7g055710 | 62.031 | 453 | 80 | 6 | 1 | 364 | 1 | 450 | 0.0 | 535 |
| MsG0880046440.01.T01 | MTR_7g070660 | 61.830 | 448 | 83 | 7 | 1 | 363 | 1 | 445 | 0.0 | 531 |
| MsG0880046440.01.T01 | MTR_7g070800 | 59.685 | 444 | 88 | 5 | 8 | 364 | 3 | 442 | 0.0 | 521 |
| MsG0880046440.01.T01 | MTR_7g070780 | 60.706 | 453 | 86 | 5 | 1 | 364 | 1 | 450 | 0.0 | 512 |
| MsG0880046440.01.T01 | MTR_7g070860 | 59.907 | 429 | 91 | 6 | 1 | 356 | 1 | 421 | 4.05e-177 | 501 |
| MsG0880046440.01.T01 | MTR_7g070930 | 58.720 | 453 | 94 | 6 | 2 | 364 | 5 | 454 | 1.23e-172 | 490 |
| MsG0880046440.01.T01 | MTR_7g070610 | 57.675 | 456 | 98 | 9 | 1 | 364 | 1 | 453 | 1.12e-171 | 488 |
| MsG0880046440.01.T01 | MTR_7g070910 | 56.388 | 454 | 105 | 4 | 1 | 364 | 1 | 451 | 2.09e-171 | 487 |
| MsG0880046440.01.T01 | MTR_7g071050 | 60.345 | 406 | 115 | 6 | 2 | 364 | 4 | 406 | 3.22e-162 | 461 |
| MsG0880046440.01.T01 | MTR_7g070940 | 55.275 | 436 | 110 | 5 | 1 | 356 | 1 | 431 | 5.14e-157 | 450 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MsG0880046440.01.T01 | AT2G15490 | 34.061 | 458 | 195 | 10 | 9 | 362 | 5 | 459 | 1.04e-76 | 244 |
| MsG0880046440.01.T01 | AT2G15490 | 33.839 | 461 | 195 | 10 | 9 | 362 | 5 | 462 | 3.66e-76 | 243 |
| MsG0880046440.01.T01 | AT2G15480 | 33.117 | 462 | 206 | 10 | 3 | 362 | 2 | 462 | 5.80e-76 | 243 |
| MsG0880046440.01.T01 | AT2G15480 | 32.456 | 456 | 210 | 10 | 3 | 363 | 2 | 454 | 6.16e-76 | 243 |
| MsG0880046440.01.T01 | AT4G34131 | 33.184 | 446 | 199 | 8 | 3 | 356 | 2 | 440 | 8.06e-76 | 242 |
| MsG0880046440.01.T01 | AT4G34135 | 32.135 | 445 | 209 | 11 | 1 | 356 | 1 | 441 | 3.92e-72 | 233 |
| MsG0880046440.01.T01 | AT2G36770 | 32.143 | 448 | 206 | 11 | 2 | 356 | 5 | 447 | 2.11e-69 | 226 |
| MsG0880046440.01.T01 | AT4G34138 | 32.391 | 460 | 203 | 13 | 8 | 364 | 8 | 462 | 2.16e-67 | 220 |
| MsG0880046440.01.T01 | AT2G36780 | 30.425 | 447 | 215 | 9 | 2 | 356 | 5 | 447 | 1.45e-65 | 216 |
| MsG0880046440.01.T01 | AT2G36750 | 30.979 | 439 | 206 | 10 | 10 | 356 | 9 | 442 | 4.20e-65 | 214 |
Find 81 sgRNAs with CRISPR-Local
Find 115 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| AAACTTAGTAAAACTACAAT+GGG | 0.395728 | 8:+73430834 | MsG0880046440.01.T01:CDS |
| AAATGATAGAGACGAGGACG+AGG | 0.590971 | 8:+73431107 | MsG0880046440.01.T01:CDS |
| AACAAAATAACATGCACAAT+TGG | 0.403192 | 8:-73430596 | None:intergenic |
| AACATTGGCCATGTGATCAT+TGG | 0.446631 | 8:-73431294 | None:intergenic |
| AACTACATGAAGTTGGTTAT+TGG | 0.174929 | 8:-73430149 | None:intergenic |
| AACTTAGTAAAACTACAATG+GGG | 0.781934 | 8:+73430835 | MsG0880046440.01.T01:CDS |
| AAGGGTGAAAGAAAGCAACA+AGG | 0.690910 | 8:+73431155 | MsG0880046440.01.T01:CDS |
| AATCGGAGGCAAGAAGTTAT+GGG | 0.408679 | 8:+73430769 | MsG0880046440.01.T01:CDS |
| AATGCTCAGAGATCCTATTG+AGG | 0.638118 | 8:+73430453 | MsG0880046440.01.T01:CDS |
| AATGTCAAAGATGTTAAAGC+TGG | 0.486509 | 8:+73430388 | MsG0880046440.01.T01:CDS |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|---|---|---|---|---|
| AAGAGACAGAATTGCTAAAT+TGG | + | Chr8:73430951-73430970 | MsG0880046440.01.T01:intron | 30.0% | |
| AATGTCAAAGATGTTAAAGC+TGG | + | Chr8:73430388-73430407 | MsG0880046440.01.T01:CDS | 30.0% | |
| ATATCAGTGACTATACAATC+TGG | - | Chr8:73430499-73430518 | None:intergenic | 30.0% | |
| CCTCTCATTGTAAAATTGTT+CGG | - | Chr8:73431322-73431341 | None:intergenic | 30.0% | |
| GAAGAAAAACTACATGAAGT+TGG | - | Chr8:73430159-73430178 | None:intergenic | 30.0% | |
| GAGCAAAAGAAAACAAGTTA+TGG | + | Chr8:73431379-73431398 | MsG0880046440.01.T01:CDS | 30.0% | |
| TAAAATTGTTCGGCAAACAT+TGG | - | Chr8:73431312-73431331 | None:intergenic | 30.0% | |
| TAACAAGGATGATGAAAGAA+AGG | + | Chr8:73430895-73430914 | MsG0880046440.01.T01:CDS | 30.0% | |
| TCAGAAACTAAATGATCATG+AGG | - | Chr8:73430634-73430653 | None:intergenic | 30.0% | |
| TCTTGAAACATTACCTCAAT+AGG | - | Chr8:73430469-73430488 | None:intergenic | 30.0% |
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| Chr8 | gene | 73430133 | 73431494 | 73430133 | ID=MsG0880046440.01;Name=MsG0880046440.01 |
| Chr8 | mRNA | 73430133 | 73431494 | 73430133 | ID=MsG0880046440.01.T01;Parent=MsG0880046440.01;Name=MsG0880046440.01.T01;_AED=0.49;_eAED=0.49;_QI=0|0|0|1|1|1|3|0|364 |
| Chr8 | exon | 73430133 | 73430615 | 73430133 | ID=MsG0880046440.01.T01:exon:11726;Parent=MsG0880046440.01.T01 |
| Chr8 | exon | 73430711 | 73430931 | 73430711 | ID=MsG0880046440.01.T01:exon:11727;Parent=MsG0880046440.01.T01 |
| Chr8 | exon | 73431104 | 73431494 | 73431104 | ID=MsG0880046440.01.T01:exon:11728;Parent=MsG0880046440.01.T01 |
| Chr8 | CDS | 73430133 | 73430615 | 73430133 | ID=MsG0880046440.01.T01:cds;Parent=MsG0880046440.01.T01 |
| Chr8 | CDS | 73430711 | 73430931 | 73430711 | ID=MsG0880046440.01.T01:cds;Parent=MsG0880046440.01.T01 |
| Chr8 | CDS | 73431104 | 73431494 | 73431104 | ID=MsG0880046440.01.T01:cds;Parent=MsG0880046440.01.T01 |
| Gene Sequence |
>MsG0880046440.01.T01 ATGGAGTCTCAACAATCCAATAACCAACTTCATGTAGTTTTTCTTCCATATCCAACTCCTGGCCATATGATTCCCATGATAGACACAGCAAGACTATTTGCAAAGCATGGTGTTAATGTTACCATCATCACTACACATGCCAATGCTTCAACATTCCAAGAATCCATAGACAGTGACTTCAATTCAGGATACTCTATCAAAACTCAGCTTATTCAGTTCCCTTCATCTCAAGTTGGTCTTCCTGATGGAATTGAAAATGTCAAAGATGTTAAAGCTGGAACATCCTCAGAAATGCTTGGTAAAATCAGTCATGGAATGTTAATGCTCAGAGATCCTATTGAGGTAATGTTTCAAGATCTTCAACCAGATTGTATAGTCACTGATATGATGATTCCTTGGACTGTGGAATCAGCTGCAAAACTAAGCATTCCTAGGCTTTACTATTACAGCTCAAGCTACTTCTCCAATTGTGCATGTTATTTTCTTCGTGATTGGGTGAGGACTACAAATGCTGCCACAGCCTATTTTGAACCAATTTTTGAATCGGAGGCAAGAAGTTATGGGACAATATGCAATAGTTTTCATGAACTTGAAAGTGATTATGAGAAACTTAGTAAAACTACAATGGGGATTAAGTCTTGGAGTGTAGGACCAGTTTCACTTGGGCTAACAAGGATGATGAAAGAAAGGCCAATAGGGGACACGAAAAATGATAGAGACGAGGACGAGGAAGGTTTCCTGCAAGATTTTGAGGAAAGGGTGAAAGAAAGCAACAAGGGCTATATCATATGGAATTGGGCACCACAGCTTCTCATATTGGATCACCCTGCAACAGGAGGAATTGTGACTCACTGTGGTTGGAACTCAACACTTGAAAGCATTAGTGTTGGTTTGCCAATGATCACATGGCCAATGTTTGCCGAACAATTTTACAATGAGAGGTTGCTTGTTGATGTTTTGAAGATAGGGGTCCCAGTGGGAGCAAAAGAAAACAAGTTATGGAATAGCTTTAGTGTAGAGGCAATGGTTAGAAGGGAAGAGATTGCAAAGGCTGCGGAGATTTTGTTGGAAATGGTCAAGACATCAAAGAAATGA |
| Protein sequence |
>MsG0880046440.01.T01 MESQQSNNQLHVVFLPYPTPGHMIPMIDTARLFAKHGVNVTIITTHANASTFQESIDSDFNSGYSIKTQLIQFPSSQVGLPDGIENVKDVKAGTSSEMLGKISHGMLMLRDPIEVMFQDLQPDCIVTDMMIPWTVESAAKLSIPRLYYYSSSYFSNCACYFLRDWVRTTNAATAYFEPIFESEARSYGTICNSFHELESDYEKLSKTTMGIKSWSVGPVSLGLTRMMKERPIGDTKNDRDEDEEGFLQDFEERVKESNKGYIIWNWAPQLLILDHPATGGIVTHCGWNSTLESISVGLPMITWPMFAEQFYNERLLVDVLKIGVPVGAKENKLWNSFSVEAMVRREEIAKAAEILLEMVKTSKK* |
