AlfalfaGEDB | MsG0280008413.01

M. sativa cultivar ZhongmuNo.1 / MsG0280008413.01

KEGG: -
GO: -
PFAM: PF13456

Nr:

Query id	Subject id	identity %	alignment length	mis matches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MsG0280008413.01.T01	RHN79743.1	88.119	101	12	0	1	101	1	101	6.61E-58	184

Swissprot:

Query id	Subject id	identity %	alignment length	mis matches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score

Trembl:

Query id	Subject id	identity %	alignment length	mis matches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MsG0280008413.01.T01	A0A396JTU0	88.119	101	12	0	1	101	1	101	3.16e-58	184

TFs/TRs:

Gene ID	Type	Classification

Protein Kinases:

Gene ID	Type	Classification

Network:

Co-expression Network

Gene1	Gene2	correlation coefficient	p_value	FDR

PPI

Gene1	Gene2	Type
MsG0780040274.01	MsG0280008413.01	PPI
MsG0780041116.01	MsG0280008413.01	PPI
MsG0280010449.01	MsG0280008413.01	PPI
MsG0780041116.01	MsG0280008413.01	PPI
MsG0380013578.01	MsG0280008413.01	PPI
MsG0280009322.01	MsG0280008413.01	PPI

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MsG0280008413.01.T01	MTR_1g034430	84.146	82	13	0	1	82	101	182	3.86e-44	141
MsG0280008413.01.T01	MTR_5g075610	61.616	99	36	1	1	99	1	97	8.75e-40	127
MsG0280008413.01.T01	MTR_1g088650	57.143	98	42	0	1	98	92	189	2.72e-36	122
MsG0280008413.01.T01	MTR_4g008270	64.103	78	28	0	1	78	16	93	4.07e-32	108
MsG0280008413.01.T01	MTR_2g059470	62.821	78	29	0	1	78	6	83	2.50e-31	105
MsG0280008413.01.T01	MTR_8g031800	62.857	70	26	0	1	70	37	106	4.91e-27	96.3
MsG0280008413.01.T01	MTR_7g063280	39.796	98	59	0	1	98	78	175	5.52e-25	92.8
MsG0280008413.01.T01	MTR_5g085210	44.545	110	38	2	1	103	125	218	8.34e-24	91.3
MsG0280008413.01.T01	MTR_8g098925	44.565	92	51	0	1	92	71	162	3.47e-23	87.8
MsG0280008413.01.T01	MTR_8g022750	57.143	70	30	0	24	93	217	286	3.09e-21	85.5
MsG0280008413.01.T01	MTR_6g016265	43.210	81	46	0	1	81	45	125	6.84e-20	78.2
MsG0280008413.01.T01	MTR_2g027020	43.434	99	52	1	2	100	35	129	1.92e-19	79.0
MsG0280008413.01.T01	MTR_0093s0070	44.156	77	43	0	1	77	129	205	1.11e-18	77.0
MsG0280008413.01.T01	MTR_3g116210	41.772	79	46	0	1	79	103	181	3.29e-18	75.5
MsG0280008413.01.T01	MTR_1g074240	54.386	57	26	0	1	57	6	62	4.37e-18	72.4
MsG0280008413.01.T01	MTR_5g037920	42.157	102	51	3	1	98	104	201	8.61e-18	75.5
MsG0280008413.01.T01	MTR_6g453270	41.772	79	46	0	1	79	306	384	2.29e-17	75.9
MsG0280008413.01.T01	MTR_0015s0120	50.000	66	33	0	1	66	299	364	6.72e-17	74.3
MsG0280008413.01.T01	MTR_1g045560	34.000	100	50	1	1	100	1	84	1.27e-16	69.3
MsG0280008413.01.T01	MTR_1g069475	40.000	80	48	0	2	81	171	250	1.94e-16	72.0
MsG0280008413.01.T01	MTR_4g029360	43.011	93	53	0	3	95	135	227	2.89e-16	71.2
MsG0280008413.01.T01	MTR_7g082130	38.235	102	38	2	1	101	50	127	6.17e-16	68.6
MsG0280008413.01.T01	MTR_8g088220	40.789	76	45	0	3	78	2	77	1.12e-15	66.2
MsG0280008413.01.T01	MTR_1g017840	44.872	78	43	0	1	78	84	161	1.31e-15	68.2
MsG0280008413.01.T01	MTR_1g045340	37.363	91	57	0	1	91	90	180	1.45e-15	68.6
MsG0280008413.01.T01	MTR_8g028785	43.590	78	44	0	24	101	287	364	2.04e-15	70.5
MsG0280008413.01.T01	MTR_2g047100	40.426	94	41	1	1	94	24	102	8.24e-15	64.7
MsG0280008413.01.T01	MTR_5g083200	36.264	91	58	0	1	91	104	194	9.04e-15	66.6
MsG0280008413.01.T01	MTR_0002s1220	36.264	91	58	0	1	91	104	194	9.04e-15	66.6
MsG0280008413.01.T01	MTR_3g111390	36.264	91	58	0	1	91	104	194	9.04e-15	66.6
MsG0280008413.01.T01	MTR_1g070820	36.264	91	58	0	1	91	104	194	9.04e-15	66.6
MsG0280008413.01.T01	MTR_6g089560	38.272	81	50	0	1	81	122	202	1.21e-14	67.0
MsG0280008413.01.T01	MTR_1g074090	48.276	58	30	0	1	58	87	144	1.25e-14	65.5
MsG0280008413.01.T01	MTR_7g013520	43.421	76	43	0	1	76	76	151	1.35e-14	66.2
MsG0280008413.01.T01	MTR_1g062840	40.541	74	44	0	17	90	2	75	1.58e-14	63.5
MsG0280008413.01.T01	MTR_5g036320	42.623	61	35	0	1	61	85	145	6.70e-14	63.5
MsG0280008413.01.T01	MTR_4g064590	44.706	85	41	2	18	100	49	129	1.72e-13	62.8
MsG0280008413.01.T01	MTR_5g069700	40.625	64	38	0	16	79	2	65	2.64e-13	60.1
MsG0280008413.01.T01	MTR_8g099580	38.462	78	48	0	1	78	212	289	4.00e-13	63.5
MsG0280008413.01.T01	MTR_1g050488	45.902	61	33	0	1	61	104	164	5.56e-13	61.6
MsG0280008413.01.T01	MTR_1g081930	36.585	82	52	0	1	82	44	125	5.77e-13	60.8
MsG0280008413.01.T01	MTR_7g053400	42.857	56	32	0	1	56	181	236	8.90e-13	62.4
MsG0280008413.01.T01	MTR_6g033225	45.763	59	32	0	1	59	76	134	1.10e-12	60.1
MsG0280008413.01.T01	MTR_8g059135	31.461	89	61	0	1	89	34	122	1.14e-12	59.7
MsG0280008413.01.T01	MTR_6g034915	33.663	101	50	1	1	101	61	144	2.16e-12	60.1
MsG0280008413.01.T01	MTR_5g049680	36.620	71	44	1	1	70	37	107	2.99e-12	58.2
MsG0280008413.01.T01	MTR_8g036780	44.898	49	27	0	1	49	40	88	6.20e-12	57.8
MsG0280008413.01.T01	MTR_5g034450	41.379	58	34	0	1	58	55	112	6.40e-12	57.8
MsG0280008413.01.T01	MTR_7g006380	33.663	101	40	1	1	101	72	145	6.80e-12	58.9
MsG0280008413.01.T01	MTR_3g017480	47.692	65	21	1	36	100	37	88	8.75e-12	57.4
MsG0280008413.01.T01	MTR_7g103930	50.847	59	19	1	43	101	17	65	1.09e-11	57.4
MsG0280008413.01.T01	MTR_3g465840	41.176	68	40	0	21	88	1	68	2.75e-11	57.0
MsG0280008413.01.T01	MTR_5g091440	41.071	56	33	0	1	56	1	56	3.25e-11	56.2
MsG0280008413.01.T01	MTR_8g038340	48.077	52	27	0	1	52	195	246	6.06e-11	57.4
MsG0280008413.01.T01	MTR_1g018300	41.975	81	41	1	1	81	69	143	9.02e-11	57.0

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score

Find 20 sgRNAs with CRISPR-Local

Find 20 sgRNAs with CRISPR-GE

CRISPR-Local

CRISPR-Local
sgRNA_sequence	on_target_score	Position	Region
TTAGATGATAGTAGCGAATT+TGG	0.171093	2:+31041840	MsG0280008413.01.T01:CDS
TCAACAGGAGGCAAGCAAAT+AGG	0.285284	2:+31041925	MsG0280008413.01.T01:CDS
CAACAGGAGGCAAGCAAATA+GGG	0.333977	2:+31041926	MsG0280008413.01.T01:CDS
TTGGTCTGGGCATGTGCCTT+AGG	0.388225	2:+31041640	None:intergenic
TAGATGATAGTAGCGAATTT+GGG	0.409597	2:+31041841	MsG0280008413.01.T01:CDS
ACTCTCATGTCGAGTTCAAC+AGG	0.427054	2:+31041910	MsG0280008413.01.T01:CDS
GCTTACACACAACTTTGGAT+TGG	0.453877	2:+31041742	MsG0280008413.01.T01:CDS
TGTTTGCTTACACACAACTT+TGG	0.454887	2:+31041737	MsG0280008413.01.T01:CDS
CAGGGCTGCCTGAGCTAACT+CGG	0.488316	2:-31041954	None:intergenic
CCCTCTTTGTGATATAGATG+TGG	0.500173	2:+31041707	MsG0280008413.01.T01:CDS
CCTCTTTGTGATATAGATGT+GGG	0.553540	2:+31041708	MsG0280008413.01.T01:CDS
CCACATCTATATCACAAAGA+GGG	0.567318	2:-31041707	None:intergenic
CTCTTTGTGATATAGATGTG+GGG	0.570420	2:+31041709	MsG0280008413.01.T01:CDS
TTGTTCTTGCAAGAACGGAA+TGG	0.581096	2:+31041679	MsG0280008413.01.T01:CDS
AAGCACCATCATCATGCCTA+AGG	0.595914	2:-31041656	None:intergenic
ATGTGCCTTAGGCATGATGA+TGG	0.597033	2:+31041651	MsG0280008413.01.T01:CDS
CCCACATCTATATCACAAAG+AGG	0.606971	2:-31041708	None:intergenic
TCTTTGTGATATAGATGTGG+GGG	0.611449	2:+31041710	MsG0280008413.01.T01:CDS
CACATCTATATCACAAAGAG+GGG	0.629857	2:-31041706	None:intergenic
CTCATGTCGAGTTCAACAGG+AGG	0.715386	2:+31041913	MsG0280008413.01.T01:CDS

CRISPR-GE

badsite warning	sgRNA_sequence	Strand	Position	Region	GC_content
	TAGATGATAGTAGCGAATTT+GGG	+	Chr2:31041841-31041860	MsG0280008413.01.T01:CDS	30.0%
	TTAGATGATAGTAGCGAATT+TGG	+	Chr2:31041840-31041859	MsG0280008413.01.T01:CDS	30.0%
	CACATCTATATCACAAAGAG+GGG	-	Chr2:31041709-31041728	None:intergenic	35.0%
	CCACATCTATATCACAAAGA+GGG	-	Chr2:31041710-31041729	None:intergenic	35.0%
	CCTCTTTGTGATATAGATGT+GGG	+	Chr2:31041708-31041727	MsG0280008413.01.T01:CDS	35.0%
	CTCTTTGTGATATAGATGTG+GGG	+	Chr2:31041709-31041728	MsG0280008413.01.T01:CDS	35.0%
	TCTTTGTGATATAGATGTGG+GGG	+	Chr2:31041710-31041729	MsG0280008413.01.T01:CDS	35.0%
	TGTTTGCTTACACACAACTT+TGG	+	Chr2:31041737-31041756	MsG0280008413.01.T01:CDS	35.0%
!	TGCTTTTGTTCTTGCAAGAA+CGG	+	Chr2:31041674-31041693	MsG0280008413.01.T01:CDS	35.0%
	CCCACATCTATATCACAAAG+AGG	-	Chr2:31041711-31041730	None:intergenic	40.0%
	CCCTCTTTGTGATATAGATG+TGG	+	Chr2:31041707-31041726	MsG0280008413.01.T01:CDS	40.0%
	GCTTACACACAACTTTGGAT+TGG	+	Chr2:31041742-31041761	MsG0280008413.01.T01:CDS	40.0%
	TTGTTCTTGCAAGAACGGAA+TGG	+	Chr2:31041679-31041698	MsG0280008413.01.T01:CDS	40.0%
	AAGCACCATCATCATGCCTA+AGG	-	Chr2:31041659-31041678	None:intergenic	45.0%
	ACTCTCATGTCGAGTTCAAC+AGG	+	Chr2:31041910-31041929	MsG0280008413.01.T01:CDS	45.0%
	ATGTGCCTTAGGCATGATGA+TGG	+	Chr2:31041651-31041670	MsG0280008413.01.T01:CDS	45.0%
	CAACAGGAGGCAAGCAAATA+GGG	+	Chr2:31041926-31041945	MsG0280008413.01.T01:CDS	45.0%
	TCAACAGGAGGCAAGCAAAT+AGG	+	Chr2:31041925-31041944	MsG0280008413.01.T01:CDS	45.0%
	TTGTGATATAGATGTGGGGG+AGG	+	Chr2:31041713-31041732	MsG0280008413.01.T01:CDS	45.0%
	CTCATGTCGAGTTCAACAGG+AGG	+	Chr2:31041913-31041932	MsG0280008413.01.T01:CDS	50.0%

Chromosome	Type	Strat	End	Strand	Name
Chr2	gene	31041651	31041962	31041651	ID=MsG0280008413.01;Name=MsG0280008413.01
Chr2	mRNA	31041651	31041962	31041651	ID=MsG0280008413.01.T01;Parent=MsG0280008413.01;Name=MsG0280008413.01.T01;_AED=0.47;_eAED=0.47;_QI=0\|-1\|0\|1\|-1\|0\|1\|0\|103
Chr2	exon	31041651	31041962	31041651	ID=MsG0280008413.01.T01:exon:6921;Parent=MsG0280008413.01.T01
Chr2	CDS	31041651	31041962	31041651	ID=MsG0280008413.01.T01:cds;Parent=MsG0280008413.01.T01

Gene Sequence	>MsG0280008413.01.T01 ATGTGCCTTAGGCATGATGATGGTGCTTTTGTTCTTGCAAGAACGGAATGGTTAGCCCCTCTTTGTGATATAGATGTGGGGGAGGTTGTTTGCTTACACACAACTTTGGATTGGATCTCTAATCAACAATTTGACAATGTCGATTTTGTTCTTGATTGTAAAAGAGTTGTTGATTGCGTCAATTCTAGCTTAGATGATAGTAGCGAATTTGGGTGTATTATTACTGCTTGTAAACAGCTTCTAGTAGATAGATTTCAGAACTCTCATGTCGAGTTCAACAGGAGGCAAGCAAATAGGGTAGCTCCGAGTTAG
Protein sequence	>MsG0280008413.01.T01 MCLRHDDGAFVLARTEWLAPLCDIDVGEVVCLHTTLDWISNQQFDNVDFVLDCKRVVDCVNSSLDDSSEFGCIITACKQLLVDRFQNSHVEFNRRQANRVAPS*