AlfalfaGEDB | MsG0280008640.01

M. sativa cultivar ZhongmuNo.1 / MsG0280008640.01

KEGG: K00789
GO: GO:0004478 GO:0005524 GO:0006556
PFAM: PF00438 PF02772 PF02773 PF17284

Nr:

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Query id	Subject id	identity %	alignment length	mis matches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MsG0280008640.01.T01	A4ULF8.1	100	392	0	0	1	392	1	392	0	814

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Swissprot:

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Query id	Subject id	identity %	alignment length	mis matches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MsG0280008640.01.T01	A4ULF8	100	392	0	0	1	392	1	392	0	814

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Trembl:

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Query id	Subject id	identity %	alignment length	mis matches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MsG0280008640.01.T01	A0A072V8Q4	99.490	392	2	0	1	392	1	392	0.0	810

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TFs/TRs:

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Gene ID	Type	Classification
No data available in table

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Protein Kinases:

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Gene ID	Type	Classification
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Network:

Co-expression Network

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Gene1	Gene2	correlation coefficient	p_value	FDR
No data available in table

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PPI

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Gene1	Gene2	Type
MsG0880047232.01	MsG0280008640.01	PPI

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Query id	Subject id	identity %	alignment length	mismatches	q. start	q. end	s. start	s. end	e-value	bit score
MsG0280008640.01.T01	MTR_2g046710	99.490	392	2	1	392	1	392	0.0	810
MsG0280008640.01.T01	MTR_4g123810	94.087	389	23	1	389	1	389	0.0	741
MsG0280008640.01.T01	MTR_7g110310	93.316	389	26	4	392	3	391	0.0	738
MsG0280008640.01.T01	MTR_7g102120	90.415	386	37	4	389	2	387	0.0	704
MsG0280008640.01.T01	MTR_1g063060	87.824	386	47	4	389	2	387	0.0	693
MsG0280008640.01.T01	MTR_1g063060	87.824	386	47	4	389	20	405	0.0	693
MsG0280008640.01.T01	MTR_5g034470	93.220	59	4	397	455	74	132	5.08e-29	117
MsG0280008640.01.T01	MTR_5g034470	94.828	58	3	398	455	75	132	7.78e-29	117
MsG0280008640.01.T01	MTR_8g063940	89.655	58	6	398	455	74	131	9.25e-27	111
MsG0280008640.01.T01	MTR_3g078190	74.576	59	15	397	455	63	121	3.03e-24	103

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Query id	Subject id	identity %	alignment length	mismatches	q. start	q. end	s. start	s. end	e-value	bit score
MsG0280008640.01.T01	AT3G17390	94.344	389	22	4	392	2	390	0.0	744
MsG0280008640.01.T01	AT1G02500	92.545	389	29	4	392	2	390	0.0	724
MsG0280008640.01.T01	AT1G02500	92.545	389	29	4	392	2	390	0.0	724
MsG0280008640.01.T01	AT4G01850	91.003	389	35	4	392	2	390	0.0	722
MsG0280008640.01.T01	AT4G01850	91.003	389	35	4	392	2	390	0.0	722
MsG0280008640.01.T01	AT2G36880	90.415	386	37	4	389	2	387	0.0	707
MsG0280008640.01.T01	AT2G36880	90.415	386	37	4	389	2	387	0.0	707
MsG0280008640.01.T01	AT1G23820	94.828	58	3	398	455	67	124	4.30e-29	117
MsG0280008640.01.T01	AT1G23820	93.220	59	4	397	455	110	168	5.75e-29	117
MsG0280008640.01.T01	AT1G70310	93.103	58	4	398	455	71	128	5.02e-28	115

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Find 137 sgRNAs with CRISPR-Local

Find 323 sgRNAs with CRISPR-GE

CRISPR-Local

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CRISPR-Local
sgRNA_sequence	on_target_score	Position	Region
AACCTTGATCTTAAGAGGGG+TGG	0.542096	2:-35582538	MsG0280008640.01.T01:CDS
AACGGCCTGAAGGGTTCAAG+TGG	0.607726	2:+35582899	None:intergenic
AACTTGCACAAAGACCAACA+TGG	0.753910	2:-35583460	MsG0280008640.01.T01:CDS
AAGATCATCATCGATACCTA+CGG	0.632923	2:-35582832	MsG0280008640.01.T01:CDS
AAGATCCAACAAAAGTCGAC+AGG	0.563704	2:-35582771	MsG0280008640.01.T01:CDS
AATGGATTTGAGGGAGAAGA+GGG	0.417140	2:-35579202	MsG0280008640.01.T01:CDS
ACAAACACGGACAATGGCTC+TGG	0.479016	2:+35582652	None:intergenic
ACATAATGGGTTACAGTTAA+TGG	0.152203	2:-35579220	MsG0280008640.01.T01:CDS
ACATCTGAATCTGTGAACGA+GGG	0.708400	2:-35583570	MsG0280008640.01.T01:CDS
ACCAAAGACCATAACCATGT+TGG	0.617396	2:+35583446	None:intergenic

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CRISPR-GE

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sgRNA_sequence	Strand	Position	Region	GC_content
AAAGTGAGGTTACAATTAGT+TGG	-	Chr2:35580568-35580587	MsG0280008640.01.T01:intron	30.0%
AAGATCAACCAGTAAATCTT+AGG	+	Chr2:35581960-35581979	None:intergenic	30.0%
AATAAATGAAAACAGGGGTA+AGG	+	Chr2:35580402-35580421	None:intergenic	30.0%
AATCTATTGCAATTCATGAG+TGG	-	Chr2:35581359-35581378	MsG0280008640.01.T01:intron	30.0%
ACAATCAACATCAAGATGTT+TGG	-	Chr2:35581190-35581209	MsG0280008640.01.T01:intron	30.0%
AGGTTTCTTTCACAATGTTA+AGG	+	Chr2:35580138-35580157	None:intergenic	30.0%
ATAAATGAAAACAGGGGTAA+GGG	+	Chr2:35580401-35580420	None:intergenic	30.0%
ATATAAAACAAGTGTGTAGC+AGG	+	Chr2:35583452-35583471	None:intergenic	30.0%
ATCTAAGTGATGAATTCGTA+AGG	-	Chr2:35581798-35581817	MsG0280008640.01.T01:intron	30.0%
ATCTGATTATCAGGATGTTA+TGG	-	Chr2:35582957-35582976	MsG0280008640.01.T01:CDS	30.0%

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Chromosome	Type	Strat	End	Strand	Name
Chr2	gene	35579131	35583616	35579131	ID=MsG0280008640.01;Name=MsG0280008640.01
Chr2	mRNA	35579131	35583616	35579131	ID=MsG0280008640.01.T01;Parent=MsG0280008640.01;Name=MsG0280008640.01.T01;_AED=0.49;_eAED=0.56;_QI=0\|0.33\|0.5\|1\|1\|1\|4\|0\|522
Chr2	exon	35582442	35583616	35582442	ID=MsG0280008640.01.T01:exon:36168;Parent=MsG0280008640.01.T01
Chr2	exon	35579760	35579823	35579760	ID=MsG0280008640.01.T01:exon:36167;Parent=MsG0280008640.01.T01
Chr2	exon	35579524	35579646	35579524	ID=MsG0280008640.01.T01:exon:36166;Parent=MsG0280008640.01.T01
Chr2	exon	35579131	35579337	35579131	ID=MsG0280008640.01.T01:exon:36165;Parent=MsG0280008640.01.T01
Chr2	CDS	35582442	35583616	35582442	ID=MsG0280008640.01.T01:cds;Parent=MsG0280008640.01.T01
Chr2	CDS	35579760	35579823	35579760	ID=MsG0280008640.01.T01:cds;Parent=MsG0280008640.01.T01
Chr2	CDS	35579524	35579646	35579524	ID=MsG0280008640.01.T01:cds;Parent=MsG0280008640.01.T01
Chr2	CDS	35579131	35579337	35579131	ID=MsG0280008640.01.T01:cds;Parent=MsG0280008640.01.T01

Gene Sequence

>MsG0280008640.01.T01

ATGGCAACAGAAACTTTCCTATACACATCTGAATCTGTGAACGAGGGACACCCCGACAAGCTTTGCGACCAGATCTCTGATGCCGTTCTTGATGCTTGCCTTGAGCAAGATCCAGACAGCAAAGTTGCATGTGAAACTTGCACAAAGACCAACATGGTTATGGTCTTTGGTGAGATCACAACAAAAGCAAATGTTGACTATGAAAAGATTGTTCGTAACACATGCAGGAACATAGGTTTTGTTTCAGATGATGTTGGTCTTGATGCTGACAACTGCAAAGTCCTTGTCAACATTGAACAACAGAGTCCTGATATTGCTCAAGGTGTTCATGGCCATCTTACAAAAAGACCTGAGGAAATTGGTGCTGGTGATCAAGGTCACATGTTCGGGTATGCTACCGATGAAACACCTGAGCTTATGCCTTTAACCCATGTTCTTGCAACCAAGTTAGGAGCTAAGCTCACTGAAGTTCGCAAGAACGGAACATGCCCTTGGTTGAGACCTGATGGAAAGACCCAAGTCACTATTGAGTATTTCAATGACAAGGGTGCCATGGTTCCGATCCGTGTCCATACCGTCCTTATCTCAACACAGCACGACGAAACTGTGACCAATGATGAAATTGCAGCTGATTTGAAACAACATGTGATCAAGCCTGTGATCCCAGAGAAGTACCTTGATGAGAAAACCATCTTCCACTTGAACCCTTCAGGCCGTTTCGTCATCGGTGGTCCTCACGGTGACGCTGGTCTAACCGGCCGCAAGATCATCATCGATACCTACGGTGGTTGGGGTGCACACGGTGGTGGTGCTTTCTCCGGTAAAGATCCAACAAAAGTCGACAGGAGTGGAGCTTACATCGTGAGACAAGCTGCAAAGAGTATTGTTGCTAATGGACTTGCTAGAAGGTGCATTGTGCAAGTTTCCTATGCTATCGGTGTTCCAGAGCCATTGTCCGTGTTTGTTGATTCTTACGGTACCGGAAAGATCCCTGATAAGGAAATCCTTAACATTGTGAAAGAAACCTTTGACTTCAGGCCAGGAATGATCTCTATCAACCTTGATCTTAAGAGGGGTGGAAATAACAGGTTTTTGAAGACTGCTGCTTATGGACATTTTGGTAGAGATGATGCTGATTTCACATGGGAAGTTGTGAAGCCACTCAAGGGTGGAAAGTATAATTGGTTCAATTTGTTGTTTCAAGGAAAATCTGATTATCAGGATGTTATGGTCTTCCAGTCAGCAACATATGGCAAGGTTCTTGTTTTGGATGGGGTCATTCAGCTCACAGAATGGGACGAGTGTGCCTACCAAGAGATGATCACTCATCTTCCTCTTTGCTCAATTCCAAACCCCAAAAAGGTTACTCATAATGTTAATGTGTCCGTCACCATGCTAACTCCTGCTACACACTTGTTTTATATTCATCATAGCAACACTGTTTTAATGGGATTTGAACATAATGGGTTACAGTTAATGGATTTGAGGGAGAAGAGGGATGAAGAGTTGAGGCGACTACTCACCAGTGTTCTAAGGAATCCACAACAACCACCACCATCATCTTCTTAA

Protein sequence

>MsG0280008640.01.T01

MATETFLYTSESVNEGHPDKLCDQISDAVLDACLEQDPDSKVACETCTKTNMVMVFGEITTKANVDYEKIVRNTCRNIGFVSDDVGLDADNCKVLVNIEQQSPDIAQGVHGHLTKRPEEIGAGDQGHMFGYATDETPELMPLTHVLATKLGAKLTEVRKNGTCPWLRPDGKTQVTIEYFNDKGAMVPIRVHTVLISTQHDETVTNDEIAADLKQHVIKPVIPEKYLDEKTIFHLNPSGRFVIGGPHGDAGLTGRKIIIDTYGGWGAHGGGAFSGKDPTKVDRSGAYIVRQAAKSIVANGLARRCIVQVSYAIGVPEPLSVFVDSYGTGKIPDKEILNIVKETFDFRPGMISINLDLKRGGNNRFLKTAAYGHFGRDDADFTWEVVKPLKGGKYNWFNLLFQGKSDYQDVMVFQSATYGKVLVLDGVIQLTEWDECAYQEMITHLPLCSIPNPKKVTHNVNVSVTMLTPATHLFYIHHSNTVLMGFEHNGLQLMDLREKRDEELRRLLTSVLRNPQQPPPSSS*