Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MsG0280008775.01.T01 | KEH38783.1 | 83 | 100 | 17 | 0 | 4 | 103 | 844 | 943 | 1.05E-48 | 176 |
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|
| Gene ID | Type | Classification |
|---|
| Gene ID | Type | Classification |
|---|
Co-expression Network
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|---|---|---|---|
| MsG0280008775.01 | MsG0580029074.01 | 0.823434 | 1.388330e-53 | 1.195198e-50 |
| MsG0280008775.01 | MsG0880043240.01 | 0.828410 | 9.129478e-55 | 9.067345e-52 |
PPI
| Gene1 | Gene2 | Type |
|---|
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MsG0280008775.01.T01 | MTR_2g079850 | 83.000 | 100 | 17 | 0 | 4 | 103 | 844 | 943 | 1.27e-52 | 176 |
| MsG0280008775.01.T01 | MTR_2g079850 | 83.000 | 100 | 17 | 0 | 4 | 103 | 996 | 1095 | 1.71e-52 | 176 |
| MsG0280008775.01.T01 | MTR_1g105085 | 89.535 | 86 | 9 | 0 | 18 | 103 | 1 | 86 | 4.60e-52 | 158 |
| MsG0280008775.01.T01 | MTR_6g009120 | 73.786 | 103 | 5 | 1 | 1 | 103 | 1 | 81 | 2.26e-48 | 149 |
| MsG0280008775.01.T01 | MTR_7g105760 | 87.654 | 81 | 10 | 0 | 23 | 103 | 175 | 255 | 4.84e-46 | 149 |
| MsG0280008775.01.T01 | MTR_6g464960 | 70.874 | 103 | 6 | 1 | 1 | 103 | 141 | 219 | 5.20e-44 | 142 |
| MsG0280008775.01.T01 | MTR_8g107170 | 88.333 | 60 | 7 | 0 | 23 | 82 | 121 | 180 | 4.41e-32 | 110 |
| MsG0280008775.01.T01 | MTR_6g466340 | 81.132 | 53 | 10 | 0 | 51 | 103 | 99 | 151 | 3.37e-26 | 95.1 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|
Find 17 sgRNAs with CRISPR-Local
Find 16 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| TTCACAGCAGTAAATATTAA+TGG | 0.224854 | 2:-38318082 | None:intergenic |
| TTTCGTAAGCTTATATTCTT+TGG | 0.238112 | 2:+38318271 | MsG0280008775.01.T01:CDS |
| GTAAATATTAATGGTTTGTT+AGG | 0.274608 | 2:-38318073 | None:intergenic |
| ATTTACTGCTGTGAATTCTA+AGG | 0.307193 | 2:+38318090 | MsG0280008775.01.T01:CDS |
| TCTTGCATTGCTTCGTTACT+CGG | 0.344703 | 2:-38318184 | None:intergenic |
| CAATCCACAACAGGAAGAAA+TGG | 0.395001 | 2:+38318209 | MsG0280008775.01.T01:CDS |
| TTATCCATTTCTTCCTGTTG+TGG | 0.432151 | 2:-38318213 | None:intergenic |
| TGTTAGTATTATTTCGAAGT+TGG | 0.439570 | 2:-38318024 | None:intergenic |
| AGCTTATATTCTTTGGAAGA+AGG | 0.441153 | 2:+38318278 | MsG0280008775.01.T01:CDS |
| GAAGAAATGGATAAATGATC+AGG | 0.451185 | 2:+38318222 | MsG0280008775.01.T01:CDS |
| TCAATGACAACTTCACTTCG+TGG | 0.472330 | 2:+38317992 | None:intergenic |
| TGCAAGAATCAATCCACAAC+AGG | 0.472379 | 2:+38318200 | MsG0280008775.01.T01:CDS |
| ATTGCTTCGTTACTCGGCGG+AGG | 0.574895 | 2:-38318178 | None:intergenic |
| TCAGGAGAACTCTTCAAAAG+TGG | 0.590064 | 2:+38318240 | MsG0280008775.01.T01:CDS |
| AATCTCTGAGACTTTAGATG+AGG | 0.603020 | 2:-38318143 | None:intergenic |
| AACTCTTCAAAAGTGGTGAG+AGG | 0.607538 | 2:+38318247 | MsG0280008775.01.T01:CDS |
| TGCATTGCTTCGTTACTCGG+CGG | 0.686151 | 2:-38318181 | None:intergenic |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|---|---|---|---|---|
| !!! | GTAAATATTAATGGTTTGTT+AGG | - | Chr2:38318076-38318095 | None:intergenic | 20.0% |
| ! | TGTTAGTATTATTTCGAAGT+TGG | - | Chr2:38318027-38318046 | None:intergenic | 25.0% |
| ! | TTCACAGCAGTAAATATTAA+TGG | - | Chr2:38318085-38318104 | None:intergenic | 25.0% |
| ! | TTTCGTAAGCTTATATTCTT+TGG | + | Chr2:38318271-38318290 | MsG0280008775.01.T01:CDS | 25.0% |
| ATTTACTGCTGTGAATTCTA+AGG | + | Chr2:38318090-38318109 | MsG0280008775.01.T01:CDS | 30.0% | |
| GAAGAAATGGATAAATGATC+AGG | + | Chr2:38318222-38318241 | MsG0280008775.01.T01:CDS | 30.0% | |
| ! | AGCTTATATTCTTTGGAAGA+AGG | + | Chr2:38318278-38318297 | MsG0280008775.01.T01:CDS | 30.0% |
| TTATCCATTTCTTCCTGTTG+TGG | - | Chr2:38318216-38318235 | None:intergenic | 35.0% | |
| !! | AATCTCTGAGACTTTAGATG+AGG | - | Chr2:38318146-38318165 | None:intergenic | 35.0% |
| AACTCTTCAAAAGTGGTGAG+AGG | + | Chr2:38318247-38318266 | MsG0280008775.01.T01:CDS | 40.0% | |
| CAATCCACAACAGGAAGAAA+TGG | + | Chr2:38318209-38318228 | MsG0280008775.01.T01:CDS | 40.0% | |
| TCAGGAGAACTCTTCAAAAG+TGG | + | Chr2:38318240-38318259 | MsG0280008775.01.T01:CDS | 40.0% | |
| TCTTGCATTGCTTCGTTACT+CGG | - | Chr2:38318187-38318206 | None:intergenic | 40.0% | |
| TGCAAGAATCAATCCACAAC+AGG | + | Chr2:38318200-38318219 | MsG0280008775.01.T01:CDS | 40.0% | |
| TGCATTGCTTCGTTACTCGG+CGG | - | Chr2:38318184-38318203 | None:intergenic | 50.0% | |
| ATTGCTTCGTTACTCGGCGG+AGG | - | Chr2:38318181-38318200 | None:intergenic | 55.0% |
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| Chr2 | gene | 38317995 | 38318306 | 38317995 | ID=MsG0280008775.01;Name=MsG0280008775.01 |
| Chr2 | mRNA | 38317995 | 38318306 | 38317995 | ID=MsG0280008775.01.T01;Parent=MsG0280008775.01;Name=MsG0280008775.01.T01;_AED=0.47;_eAED=0.47;_QI=0|-1|0|1|-1|1|1|0|103 |
| Chr2 | exon | 38317995 | 38318306 | 38317995 | ID=MsG0280008775.01.T01:exon:57907;Parent=MsG0280008775.01.T01 |
| Chr2 | CDS | 38317995 | 38318306 | 38317995 | ID=MsG0280008775.01.T01:cds;Parent=MsG0280008775.01.T01 |
| Gene Sequence |
| Protein sequence |
