Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MsG0580029074.01.T01 | KEH38783.1 | 85 | 100 | 15 | 0 | 4 | 103 | 844 | 943 | 8.78E-50 | 179 |
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MsG0580029074.01.T01 | A0A072VBH3 | 85.000 | 100 | 15 | 0 | 4 | 103 | 844 | 943 | 4.19e-50 | 179 |
| Gene ID | Type | Classification |
|---|
| Gene ID | Type | Classification |
|---|
Co-expression Network
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|---|---|---|---|
| MsG0580029074.01 | MsG0880043240.01 | 0.920424 | 1.303144e-87 | 4.415658e-83 |
| MsG0280008775.01 | MsG0580029074.01 | 0.823434 | 1.388330e-53 | 1.195198e-50 |
PPI
| Gene1 | Gene2 | Type |
|---|
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MsG0580029074.01.T01 | MTR_2g079850 | 85.000 | 100 | 15 | 0 | 4 | 103 | 844 | 943 | 1.06e-53 | 179 |
| MsG0580029074.01.T01 | MTR_2g079850 | 85.000 | 100 | 15 | 0 | 4 | 103 | 996 | 1095 | 1.55e-53 | 179 |
| MsG0580029074.01.T01 | MTR_1g105085 | 91.860 | 86 | 7 | 0 | 18 | 103 | 1 | 86 | 2.89e-53 | 161 |
| MsG0580029074.01.T01 | MTR_6g009120 | 75.728 | 103 | 3 | 1 | 1 | 103 | 1 | 81 | 8.49e-50 | 152 |
| MsG0580029074.01.T01 | MTR_7g105760 | 88.889 | 81 | 9 | 0 | 23 | 103 | 175 | 255 | 1.78e-46 | 149 |
| MsG0580029074.01.T01 | MTR_6g464960 | 71.845 | 103 | 5 | 1 | 1 | 103 | 141 | 219 | 1.98e-44 | 143 |
| MsG0580029074.01.T01 | MTR_8g107170 | 88.333 | 60 | 7 | 0 | 23 | 82 | 121 | 180 | 5.03e-32 | 110 |
| MsG0580029074.01.T01 | MTR_6g466340 | 83.019 | 53 | 9 | 0 | 51 | 103 | 99 | 151 | 1.14e-26 | 96.3 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|
Find 17 sgRNAs with CRISPR-Local
Find 16 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| TTCACAGCAGTAAATATTAA+TGG | 0.224854 | 5:-91442115 | None:intergenic |
| TTTCGTAAGCTTATATTCTT+TGG | 0.238112 | 5:+91442304 | MsG0580029074.01.T01:CDS |
| GTAAATATTAATGGTTTGTT+AGG | 0.274608 | 5:-91442106 | None:intergenic |
| ATTTACTGCTGTGAATTCTA+AGG | 0.307193 | 5:+91442123 | MsG0580029074.01.T01:CDS |
| TCTTGCATTGCTTCGTTACT+CGG | 0.344703 | 5:-91442217 | None:intergenic |
| CAATCCACAACAGGAAGAAA+TGG | 0.395001 | 5:+91442242 | MsG0580029074.01.T01:CDS |
| TTATCCATTTCTTCCTGTTG+TGG | 0.432151 | 5:-91442246 | None:intergenic |
| TGTTAGTATTATTTCGAAGT+TGG | 0.439570 | 5:-91442057 | None:intergenic |
| AGCTTATATTCTTTGGAAGA+AGG | 0.441153 | 5:+91442311 | MsG0580029074.01.T01:CDS |
| TCAATGACAACTTCACTTCG+TGG | 0.472330 | 5:+91442025 | None:intergenic |
| TGCAAGAATCAATCCACAAC+AGG | 0.472379 | 5:+91442233 | MsG0580029074.01.T01:CDS |
| ATTGCTTCGTTACTCGGCGG+AGG | 0.574895 | 5:-91442211 | None:intergenic |
| GAAGAAATGGATAAATGATG+AGG | 0.594005 | 5:+91442255 | MsG0580029074.01.T01:CDS |
| AATCTCTGAGACTTTAGATG+AGG | 0.603020 | 5:-91442176 | None:intergenic |
| TGAGGAGAACTCTTCAAAAG+TGG | 0.605491 | 5:+91442273 | MsG0580029074.01.T01:CDS |
| AACTCTTCAAAAGTGGTGAG+AGG | 0.607538 | 5:+91442280 | MsG0580029074.01.T01:CDS |
| TGCATTGCTTCGTTACTCGG+CGG | 0.686151 | 5:-91442214 | None:intergenic |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|---|---|---|---|---|
| !!! | GTAAATATTAATGGTTTGTT+AGG | - | Chr5:91442109-91442128 | None:intergenic | 20.0% |
| ! | TGTTAGTATTATTTCGAAGT+TGG | - | Chr5:91442060-91442079 | None:intergenic | 25.0% |
| ! | TTCACAGCAGTAAATATTAA+TGG | - | Chr5:91442118-91442137 | None:intergenic | 25.0% |
| ! | TTTCGTAAGCTTATATTCTT+TGG | + | Chr5:91442304-91442323 | MsG0580029074.01.T01:CDS | 25.0% |
| ATTTACTGCTGTGAATTCTA+AGG | + | Chr5:91442123-91442142 | MsG0580029074.01.T01:CDS | 30.0% | |
| GAAGAAATGGATAAATGATG+AGG | + | Chr5:91442255-91442274 | MsG0580029074.01.T01:CDS | 30.0% | |
| ! | AGCTTATATTCTTTGGAAGA+AGG | + | Chr5:91442311-91442330 | MsG0580029074.01.T01:CDS | 30.0% |
| TTATCCATTTCTTCCTGTTG+TGG | - | Chr5:91442249-91442268 | None:intergenic | 35.0% | |
| !! | AATCTCTGAGACTTTAGATG+AGG | - | Chr5:91442179-91442198 | None:intergenic | 35.0% |
| AACTCTTCAAAAGTGGTGAG+AGG | + | Chr5:91442280-91442299 | MsG0580029074.01.T01:CDS | 40.0% | |
| CAATCCACAACAGGAAGAAA+TGG | + | Chr5:91442242-91442261 | MsG0580029074.01.T01:CDS | 40.0% | |
| TCTTGCATTGCTTCGTTACT+CGG | - | Chr5:91442220-91442239 | None:intergenic | 40.0% | |
| TGAGGAGAACTCTTCAAAAG+TGG | + | Chr5:91442273-91442292 | MsG0580029074.01.T01:CDS | 40.0% | |
| TGCAAGAATCAATCCACAAC+AGG | + | Chr5:91442233-91442252 | MsG0580029074.01.T01:CDS | 40.0% | |
| TGCATTGCTTCGTTACTCGG+CGG | - | Chr5:91442217-91442236 | None:intergenic | 50.0% | |
| ATTGCTTCGTTACTCGGCGG+AGG | - | Chr5:91442214-91442233 | None:intergenic | 55.0% |
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| Chr5 | gene | 91442028 | 91442339 | 91442028 | ID=MsG0580029074.01;Name=MsG0580029074.01 |
| Chr5 | mRNA | 91442028 | 91442339 | 91442028 | ID=MsG0580029074.01.T01;Parent=MsG0580029074.01;Name=MsG0580029074.01.T01;_AED=0.47;_eAED=0.47;_QI=0|-1|0|1|-1|1|1|0|103 |
| Chr5 | exon | 91442028 | 91442339 | 91442028 | ID=MsG0580029074.01.T01:exon:17759;Parent=MsG0580029074.01.T01 |
| Chr5 | CDS | 91442028 | 91442339 | 91442028 | ID=MsG0580029074.01.T01:cds;Parent=MsG0580029074.01.T01 |
| Gene Sequence |
| Protein sequence |
