AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar ZhongmuNo.1 / MsG0280008954.01

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MsG0280008954.01.T01 MTR_2g445210 97.090 378 6 2 1 376 1 375 0.0 751
MsG0280008954.01.T01 MTR_2g445210 97.090 378 6 2 1 376 1 375 0.0 750
MsG0280008954.01.T01 MTR_7g026230 28.150 373 201 8 6 376 8 315 1.20e-45 160
MsG0280008954.01.T01 MTR_2g008050 27.346 373 204 7 6 376 8 315 2.20e-45 160
MsG0280008954.01.T01 MTR_6g004000 26.542 373 207 7 6 376 8 315 3.52e-44 157
MsG0280008954.01.T01 MTR_3g095530 27.346 373 204 7 6 376 8 315 3.75e-44 157
MsG0280008954.01.T01 MTR_7g093260 27.056 377 208 7 2 376 4 315 4.97e-44 156
MsG0280008954.01.T01 MTR_0015s0130 26.810 373 206 7 6 376 8 315 3.43e-43 154
MsG0280008954.01.T01 MTR_2g096840 26.810 373 206 7 6 376 8 315 4.01e-43 154
MsG0280008954.01.T01 MTR_7g027185 25.258 388 199 8 4 376 3 314 7.34e-35 132
MsG0280008954.01.T01 MTR_7g027185 25.258 388 199 8 4 376 3 314 9.59e-35 132
MsG0280008954.01.T01 MTR_7g027185 25.065 387 199 10 4 375 3 313 9.23e-32 122
MsG0280008954.01.T01 MTR_8g089630 25.686 401 213 11 2 376 4 345 1.02e-26 110
MsG0280008954.01.T01 MTR_8g464900 21.065 413 241 10 7 376 9 379 1.56e-18 87.0
MsG0280008954.01.T01 MTR_5g066400 21.014 414 241 11 7 376 9 380 5.45e-17 82.4
MsG0280008954.01.T01 MTR_5g066400 21.014 414 241 11 7 376 9 380 7.72e-17 82.0
MsG0280008954.01.T01 MTR_3g030550 21.984 373 211 9 6 376 2 296 5.71e-15 75.9
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MsG0280008954.01.T01 AT3G33520 65.323 372 114 3 5 376 2 358 2.13e-176 497
MsG0280008954.01.T01 AT3G53750 27.078 373 205 7 6 376 8 315 4.02e-45 159
MsG0280008954.01.T01 AT3G53750 27.078 373 205 7 6 376 8 315 4.02e-45 159
MsG0280008954.01.T01 AT2G37620 27.078 373 205 7 6 376 8 315 4.02e-45 159
MsG0280008954.01.T01 AT2G37620 27.078 373 205 7 6 376 8 315 4.02e-45 159
MsG0280008954.01.T01 AT2G37620 27.078 373 205 7 6 376 8 315 4.02e-45 159
MsG0280008954.01.T01 AT2G37620 27.078 373 205 7 6 376 8 315 4.02e-45 159
MsG0280008954.01.T01 AT3G18780 27.882 373 202 7 6 376 8 315 5.85e-45 159
MsG0280008954.01.T01 AT3G18780 27.882 373 202 7 6 376 8 315 5.85e-45 159
MsG0280008954.01.T01 AT1G49240 27.882 373 202 7 6 376 8 315 6.30e-45 159
MsG0280008954.01.T01 AT3G18780 27.882 373 202 7 6 376 8 315 7.01e-45 159
MsG0280008954.01.T01 AT3G12110 27.346 373 204 7 6 376 8 315 1.02e-44 158
MsG0280008954.01.T01 AT2G42100 26.455 378 211 7 1 376 4 316 6.48e-44 156
MsG0280008954.01.T01 AT5G09810 27.078 373 205 7 6 376 8 315 4.42e-43 154
MsG0280008954.01.T01 AT2G42090 25.876 371 203 9 8 376 4 304 2.28e-41 149
MsG0280008954.01.T01 AT3G46520 25.737 373 210 7 6 376 8 315 4.01e-40 146
MsG0280008954.01.T01 AT3G46520 25.737 373 210 7 6 376 8 315 4.01e-40 146
MsG0280008954.01.T01 AT5G59370 25.737 373 210 7 6 376 8 315 4.14e-40 146
MsG0280008954.01.T01 AT5G59370 25.737 373 210 7 6 376 8 315 4.14e-40 146
MsG0280008954.01.T01 AT3G27000 25.737 373 210 10 7 376 6 314 2.07e-32 125
MsG0280008954.01.T01 AT2G42170 26.471 306 159 6 72 376 27 267 4.65e-30 118
MsG0280008954.01.T01 AT2G42170 26.471 306 159 6 72 376 27 267 4.65e-30 118
MsG0280008954.01.T01 AT2G42170 26.471 306 159 6 72 376 27 267 6.68e-30 117
MsG0280008954.01.T01 AT2G42170 26.471 306 159 6 72 376 27 267 6.68e-30 117
MsG0280008954.01.T01 AT1G13180 24.566 403 213 8 3 376 4 344 1.23e-27 113
MsG0280008954.01.T01 AT1G18450 22.892 415 228 9 7 376 9 376 9.40e-24 102
MsG0280008954.01.T01 AT2G42170 25.822 213 104 3 164 376 11 169 1.01e-18 84.7

Find 122 sgRNAs with CRISPR-Local

Find 163 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
TGCAGTCTCGTTGTTGATTC+TGG 0.128917 2:-42217937 MsG0280008954.01.T01:CDS
GATCTTCCGTCCAGCTGATT+TGG 0.282536 2:-42217362 MsG0280008954.01.T01:CDS
GAAGGGGTGTTGATAGGGTT+TGG 0.293126 2:+42218279 None:intergenic
GAGGAGGAAGGGGTGTTGAT+AGG 0.314013 2:+42218273 None:intergenic
AGATACCTGGTTGCTTGTTC+CGG 0.339070 2:+42217613 None:intergenic
ATCTTCCGTCCAGCTGATTT+GGG 0.343454 2:-42217361 MsG0280008954.01.T01:intron
AAGGGGTGTTGATAGGGTTT+GGG 0.350492 2:+42218280 None:intergenic
CAGATATCGCGTTGGAGGTC+GGG 0.352966 2:+42218177 None:intergenic
GCTGTTAAGCGTATCGATTT+AGG 0.353517 2:-42217862 MsG0280008954.01.T01:CDS
CACTCTTTCTAGCAAAGTTA+AGG 0.363869 2:+42217710 None:intergenic
GTCGCGTTCGCCGCCGAAAC+CGG 0.382775 2:+42218362 None:intergenic
CCGACCTCCAACGCGATATC+TGG 0.385499 2:-42218176 MsG0280008954.01.T01:CDS
AGGGGTTTGTAGAGACAGTT+GGG 0.395072 2:+42218324 None:intergenic
AGGAGGAAGGGGTGTTGATA+GGG 0.403649 2:+42218274 None:intergenic
GATGGGGCGGCGAACGGAGG+CGG 0.405792 2:+42218221 None:intergenic
CTTAACTTTGCTAGAAAGAG+TGG 0.414055 2:-42217709 MsG0280008954.01.T01:CDS
GTCTTGTTCGGAGGAGGAGG+AGG 0.416962 2:+42218251 None:intergenic
CTTTCGTTCTGTTAATGTCA+TGG 0.422962 2:-42217800 MsG0280008954.01.T01:CDS
AAGGCCATTGGGACGTTGGC+TGG 0.429134 2:+42217975 None:intergenic
TTGTTCGGAGGAGGAGGAGG+AGG 0.431548 2:+42218254 None:intergenic
AAAAGGGTTTGTTAAATATC+CGG 0.434138 2:-42217632 MsG0280008954.01.T01:CDS
CCAGATATCGCGTTGGAGGT+CGG 0.435023 2:+42218176 None:intergenic
ACTGCTCGTACAATGCATTC+TGG 0.435495 2:+42216959 None:intergenic
GGGGCGGCGAACGGAGGCGG+AGG 0.435672 2:+42218224 None:intergenic
GGCTTTAGAGAGAAGGCCAT+TGG 0.444232 2:+42217963 None:intergenic
TTTGTGTGCGTAACACCATC+AGG 0.445685 2:+42217652 None:intergenic
AATGGAAGGGAGAGTGAAGA+GGG 0.448911 2:+42218089 None:intergenic
GATGGTGTTACGCACACAAA+AGG 0.449644 2:-42217649 MsG0280008954.01.T01:CDS
GAGGGGTTTGTAGAGACAGT+TGG 0.455834 2:+42218323 None:intergenic
GGACTAATAAAAGCCGGTTT+CGG 0.458262 2:-42218375 MsG0280008954.01.T01:CDS
GAGGTCTTGTTCGGAGGAGG+AGG 0.465259 2:+42218248 None:intergenic
GGCGGAGGTGAGGTCTTGTT+CGG 0.475189 2:+42218239 None:intergenic
AGGGTTGATGCGGAGGATAG+AGG 0.482297 2:+42218140 None:intergenic
GAGTGAAGAGGGGTTGAGTG+AGG 0.485893 2:+42218100 None:intergenic
ATCGATGGGGCGGCGAACGG+AGG 0.486424 2:+42218218 None:intergenic
ATTAAGTAGCCGCGATCGAT+GGG 0.489209 2:+42218204 None:intergenic
GGTGAGGTCTTGTTCGGAGG+AGG 0.489209 2:+42218245 None:intergenic
TGCATTCTGGTAGCCCAGCC+TGG 0.490741 2:+42216972 None:intergenic
AGGTATCTTGCTGATGGTAA+AGG 0.490772 2:-42217598 MsG0280008954.01.T01:CDS
AGGATGAAGATGCGGATGAC+AGG 0.500447 2:+42216931 None:intergenic
AAAGGAATGAAGGACGATAT+CGG 0.501369 2:-42217580 MsG0280008954.01.T01:CDS
GATTAAGTAGCCGCGATCGA+TGG 0.502897 2:+42218203 None:intergenic
AGAGTAAAATTCTGAAAGAC+AGG 0.502945 2:+42217892 None:intergenic
ATCAACAACGAGACTGCATT+GGG 0.505503 2:+42217942 None:intergenic
GCTGGACGGAAGATCATCTC+GGG 0.507710 2:+42217370 None:intergenic
GGAGGAGAGAGGATTGAGAA+GGG 0.508677 2:+42218121 None:intergenic
AGGAGGAGAGAGGATTGAGA+AGG 0.511930 2:+42218120 None:intergenic
TGGAGGGAGTGTTGAATGGA+AGG 0.513522 2:+42218075 None:intergenic
CAGTTGGGGAGTATAGTTGA+TGG 0.514674 2:+42218339 None:intergenic
TTCCTTGAGGTAATTAGTCA+AGG 0.516089 2:+42217831 None:intergenic
TTGGTGGAGGGAGTGTTGAA+TGG 0.517894 2:+42218071 None:intergenic
GGGTGTTGATAGGGTTTGGG+TGG 0.520947 2:+42218283 None:intergenic
AATCAACAACGAGACTGCAT+TGG 0.521172 2:+42217941 None:intergenic
AGGATTGAGAAGGGTTGATG+CGG 0.527563 2:+42218130 None:intergenic
AGTTACCTTTCATAGAGTAC+AGG 0.530261 2:+42216911 None:intergenic
AGATATCGCGTTGGAGGTCG+GGG 0.530773 2:+42218178 None:intergenic
TGAAGAGGGGTTGAGTGAGG+AGG 0.533109 2:+42218103 None:intergenic
GCTTTAGAGAGAAGGCCATT+GGG 0.533569 2:+42217964 None:intergenic
CTTCGAAGACGAGTTGGTGG+AGG 0.533716 2:+42218058 None:intergenic
TGCGGAGGATAGAGGTGAAG+AGG 0.537752 2:+42218148 None:intergenic
TGCTGATGGTAAAGGAATGA+AGG 0.539461 2:-42217590 MsG0280008954.01.T01:CDS
TTTGCAGGAATGAACCAGGC+TGG 0.540010 2:-42216986 MsG0280008954.01.T01:intron
TGCATTGGGCTTTAGAGAGA+AGG 0.542055 2:+42217956 None:intergenic
GAATGGAAGGGAGAGTGAAG+AGG 0.545075 2:+42218088 None:intergenic
GCGATCGATGGGGCGGCGAA+CGG 0.546284 2:+42218215 None:intergenic
ATGGTGTTACGCACACAAAA+GGG 0.548709 2:-42217648 MsG0280008954.01.T01:CDS
AAAGACATACCCAAATCAGC+TGG 0.551828 2:+42217352 None:intergenic
AATCTTCGAAGACGAGTTGG+TGG 0.552129 2:+42218055 None:intergenic
CTCGGCAAAGTGAGGAAACA+AGG 0.552717 2:+42216759 None:intergenic
AGCTGGACGGAAGATCATCT+CGG 0.554696 2:+42217369 None:intergenic
ATTGAGAAGGGTTGATGCGG+AGG 0.554748 2:+42218133 None:intergenic
TTTACCATCAGCAAGATACC+TGG 0.560469 2:+42217600 None:intergenic
GGAGGTGAGGTCTTGTTCGG+AGG 0.560747 2:+42218242 None:intergenic
GATTTCTAAACAACAACCTC+AGG 0.562433 2:-42217545 MsG0280008954.01.T01:CDS
GGCGAACGGAGGCGGAGGTG+AGG 0.563818 2:+42218229 None:intergenic
TTGCAGGAATGAACCAGGCT+GGG 0.564309 2:-42216985 MsG0280008954.01.T01:intron
TGAAATCTTCGAAGACGAGT+TGG 0.566448 2:+42218052 None:intergenic
GGGTTGAGTGAGGAGGAGAG+AGG 0.567804 2:+42218110 None:intergenic
TTCATCCTGTACTCTATGAA+AGG 0.569693 2:-42216916 MsG0280008954.01.T01:intron
AGAGAAGGCCATTGGGACGT+TGG 0.573577 2:+42217971 None:intergenic
AATTTGATTTGACGAATGAA+CGG 0.577603 2:-42217402 MsG0280008954.01.T01:CDS
ACTTTCTGTCTGTTGTCCTG+AGG 0.580477 2:+42217529 None:intergenic
GGGACCAGATATCGCGTTGG+AGG 0.584148 2:+42218172 None:intergenic
CATGACATTAACAGAACGAA+AGG 0.585178 2:+42217801 None:intergenic
TTAACTTTGCTAGAAAGAGT+GGG 0.586299 2:-42217708 MsG0280008954.01.T01:CDS
TACAATCTCTCGGCAAAGTG+AGG 0.588230 2:+42216751 None:intergenic
GGACGATATCGGTGATCACG+AGG 0.588468 2:-42217569 MsG0280008954.01.T01:CDS
TGTACGTATGTTCTTCCTGA+TGG 0.589066 2:-42217667 MsG0280008954.01.T01:CDS
CTAATAAAAGCCGGTTTCGG+CGG 0.590675 2:-42218372 MsG0280008954.01.T01:CDS
ATGATTTGCAGGAATGAACC+AGG 0.593073 2:-42216990 MsG0280008954.01.T01:intron
GGCGGAGGACTAATAAAAGC+CGG 0.605011 2:-42218381 MsG0280008954.01.T01:CDS
GACGTTGGCTGGTTTCGTAG+AGG 0.606408 2:+42217986 None:intergenic
GTTCGCCGCCCCATCGATCG+CGG 0.606492 2:-42218213 MsG0280008954.01.T01:CDS
TGGTTTCGTAGAGGTGAACA+AGG 0.607528 2:+42217995 None:intergenic
TTCGAAGACGAGTTGGTGGA+GGG 0.610934 2:+42218059 None:intergenic
AGCCTTGACTAATTACCTCA+AGG 0.613224 2:-42217833 MsG0280008954.01.T01:CDS
TCGTAGAGGTGAACAAGGGA+AGG 0.613318 2:+42218000 None:intergenic
AATATCCGGAACAAGCAACC+AGG 0.613653 2:-42217618 MsG0280008954.01.T01:CDS
AGTTGGGGAGTATAGTTGAT+GGG 0.614887 2:+42218340 None:intergenic
GCAACCAGGTATCTTGCTGA+TGG 0.616582 2:-42217604 MsG0280008954.01.T01:CDS
GACAGGCGAAGCGTGTGTGA+AGG 0.616585 2:+42217909 None:intergenic
TGAATCAGCGACGTAAAGAG+AGG 0.617075 2:+42218023 None:intergenic
GGAGGATAGAGGTGAAGAGG+TGG 0.622180 2:+42218151 None:intergenic
AGTAGCCGCGATCGATGGGG+CGG 0.632771 2:+42218208 None:intergenic
GAGGATAGAGGTGAAGAGGT+GGG 0.635597 2:+42218152 None:intergenic
GTTGTTCTAGACAACGGCGG+AGG 0.638316 2:-42218396 MsG0280008954.01.T01:CDS
GAAACCAGCCAACGTCCCAA+TGG 0.640767 2:-42217979 MsG0280008954.01.T01:CDS
ATGGAAGGGAGAGTGAAGAG+GGG 0.643796 2:+42218090 None:intergenic
GGAGGGAGTGTTGAATGGAA+GGG 0.646444 2:+42218076 None:intergenic
TAGAGTACAGGATGAAGATG+CGG 0.648626 2:+42216923 None:intergenic
GAAAGGAAATAAGTTCCTTG+AGG 0.652884 2:+42217818 None:intergenic
GGTGGGACCAGATATCGCGT+TGG 0.659285 2:+42218169 None:intergenic
GGGGTTTGTAGAGACAGTTG+GGG 0.662639 2:+42218325 None:intergenic
GTTAAGCGTATCGATTTAGG+TGG 0.662757 2:-42217859 MsG0280008954.01.T01:CDS
ACATACCCAAATCAGCTGGA+CGG 0.678093 2:+42217356 None:intergenic
CAACATGTCATCAAGCACGC+AGG 0.680616 2:-42218421 MsG0280008954.01.T01:exon
CAGGTGGTTGTTCTAGACAA+CGG 0.698365 2:-42218402 MsG0280008954.01.T01:CDS
TTAAGTAGCCGCGATCGATG+GGG 0.708150 2:+42218205 None:intergenic
CATGTCATCAAGCACGCAGG+TGG 0.722662 2:-42218418 MsG0280008954.01.T01:CDS
CTGGACGGAAGATCATCTCG+GGG 0.733148 2:+42217371 None:intergenic
GTGGTTGTTCTAGACAACGG+CGG 0.756595 2:-42218399 MsG0280008954.01.T01:CDS
GGTTTCGTAGAGGTGAACAA+GGG 0.756829 2:+42217996 None:intergenic

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!!! AAAAAATGTATTTGTTTTGT+TGG - Chr2:42217686-42217705 MsG0280008954.01.T01:CDS 15.0%
!!! TTTTATTATTGATGATGTTA+AGG - Chr2:42217413-42217432 MsG0280008954.01.T01:intron 15.0%
!!! TGGGATTTTAGTTAAATATT+TGG - Chr2:42218065-42218084 MsG0280008954.01.T01:CDS 20.0%
! TGATTGCAACTACAATATTA+AGG + Chr2:42218019-42218038 None:intergenic 25.0%
!! TTAGGTTTTTCTTATGTTCT+CGG - Chr2:42218154-42218173 MsG0280008954.01.T01:CDS 25.0%
!!! AAAAGGGTTTGTTAAATATC+CGG - Chr2:42217551-42217570 MsG0280008954.01.T01:CDS 25.0%
!!! AATTTGATTTGACGAATGAA+CGG - Chr2:42217781-42217800 MsG0280008954.01.T01:CDS 25.0%
AGAATGCAATACAGAACATT+GGG - Chr2:42218046-42218065 MsG0280008954.01.T01:CDS 30.0%
ATAAGAAAAACCTAACACGA+AGG + Chr2:42218149-42218168 None:intergenic 30.0%
GTGAAATGTTGTATTCGAAT+GGG - Chr2:42217946-42217965 MsG0280008954.01.T01:CDS 30.0%
TAAGAAAAACCTAACACGAA+GGG + Chr2:42218148-42218167 None:intergenic 30.0%
TACTCTATGAAAGGTAACTT+TGG - Chr2:42218276-42218295 MsG0280008954.01.T01:CDS 30.0%
TAGAATGCAATACAGAACAT+TGG - Chr2:42218045-42218064 MsG0280008954.01.T01:CDS 30.0%
TGAAATGTTGTATTCGAATG+GGG - Chr2:42217947-42217966 MsG0280008954.01.T01:CDS 30.0%
TGCACAACATGATTTCAATA+AGG - Chr2:42217896-42217915 MsG0280008954.01.T01:CDS 30.0%
TGTGAAATGTTGTATTCGAA+TGG - Chr2:42217945-42217964 MsG0280008954.01.T01:CDS 30.0%
TTAACTTTGCTAGAAAGAGT+GGG - Chr2:42217475-42217494 MsG0280008954.01.T01:intron 30.0%
! AGAGTAAAATTCTGAAAGAC+AGG + Chr2:42217294-42217313 None:intergenic 30.0%
! TGTTGAACAAAATGATTTGC+AGG - Chr2:42218182-42218201 MsG0280008954.01.T01:CDS 30.0%
!!! GACGTTGTGTTTACTTTTTT+TGG - Chr2:42218375-42218394 MsG0280008954.01.T01:CDS 30.0%
!!! TTTGGCAGCATTATTTTAAC+TGG - Chr2:42218393-42218412 MsG0280008954.01.T01:CDS 30.0%
AAAGGAATGAAGGACGATAT+CGG - Chr2:42217603-42217622 MsG0280008954.01.T01:CDS 35.0%
AAAGGCAACAATTCACAAAC+AGG + Chr2:42218331-42218350 None:intergenic 35.0%
AACAGGGTCATGAAATCAAA+TGG + Chr2:42218314-42218333 None:intergenic 35.0%
AAGAAAAACCTAACACGAAG+GGG + Chr2:42218147-42218166 None:intergenic 35.0%
AAGGCAACAATTCACAAACA+GGG + Chr2:42218330-42218349 None:intergenic 35.0%
AGTTACCTTTCATAGAGTAC+AGG + Chr2:42218275-42218294 None:intergenic 35.0%
ATTATTTACCCCTTCGTGTT+AGG - Chr2:42218136-42218155 MsG0280008954.01.T01:CDS 35.0%
CATGACATTAACAGAACGAA+AGG + Chr2:42217385-42217404 None:intergenic 35.0%
CTTAACTTTGCTAGAAAGAG+TGG - Chr2:42217474-42217493 MsG0280008954.01.T01:intron 35.0%
CTTTCGTTCTGTTAATGTCA+TGG - Chr2:42217383-42217402 MsG0280008954.01.T01:CDS 35.0%
GAAAGGAAATAAGTTCCTTG+AGG + Chr2:42217368-42217387 None:intergenic 35.0%
GATTTCTAAACAACAACCTC+AGG - Chr2:42217638-42217657 MsG0280008954.01.T01:CDS 35.0%
TTCATCCTGTACTCTATGAA+AGG - Chr2:42218267-42218286 MsG0280008954.01.T01:CDS 35.0%
TTCCTTGAGGTAATTAGTCA+AGG + Chr2:42217355-42217374 None:intergenic 35.0%
TTCTTAAGTGGATGCAAATG+AGG - Chr2:42218097-42218116 MsG0280008954.01.T01:CDS 35.0%
TTTCTTGCTTTGTGTTCTCT+AGG - Chr2:42217758-42217777 MsG0280008954.01.T01:CDS 35.0%
! CACTCTTTCTAGCAAAGTTA+AGG + Chr2:42217476-42217495 None:intergenic 35.0%
!! TGGTTGATGAGTTTCTTAAG+TGG - Chr2:42218085-42218104 MsG0280008954.01.T01:CDS 35.0%
AAACACAACGTCAAAAGACG+AGG + Chr2:42218367-42218386 None:intergenic 40.0%
AAAGACATACCCAAATCAGC+TGG + Chr2:42217834-42217853 None:intergenic 40.0%
AACACAACGTCAAAAGACGA+GGG + Chr2:42218366-42218385 None:intergenic 40.0%
AATCAACAACGAGACTGCAT+TGG + Chr2:42217245-42217264 None:intergenic 40.0%
AGCCTTGACTAATTACCTCA+AGG - Chr2:42217350-42217369 MsG0280008954.01.T01:intron 40.0%
AGGTATCTTGCTGATGGTAA+AGG - Chr2:42217585-42217604 MsG0280008954.01.T01:CDS 40.0%
ATCAACAACGAGACTGCATT+GGG + Chr2:42217244-42217263 None:intergenic 40.0%
GGGTCATGAAATCAAATGGT+TGG + Chr2:42218310-42218329 None:intergenic 40.0%
TAGAGTACAGGATGAAGATG+CGG + Chr2:42218263-42218282 None:intergenic 40.0%
TGAAATCTTCGAAGACGAGT+TGG + Chr2:42217134-42217153 None:intergenic 40.0%
TGCTGATGGTAAAGGAATGA+AGG - Chr2:42217593-42217612 MsG0280008954.01.T01:CDS 40.0%
TGTACGTATGTTCTTCCTGA+TGG - Chr2:42217516-42217535 MsG0280008954.01.T01:CDS 40.0%
TTTACCATCAGCAAGATACC+TGG + Chr2:42217586-42217605 None:intergenic 40.0%
! AGTTGGGGAGTATAGTTGAT+GGG + Chr2:42216846-42216865 None:intergenic 40.0%
! ATGATTTGCAGGAATGAACC+AGG - Chr2:42218193-42218212 MsG0280008954.01.T01:CDS 40.0%
! GGACTAATAAAAGCCGGTTT+CGG - Chr2:42216808-42216827 MsG0280008954.01.T01:intron 40.0%
!! ATGGTGTTACGCACACAAAA+GGG - Chr2:42217535-42217554 MsG0280008954.01.T01:CDS 40.0%
!! GCTGTTAAGCGTATCGATTT+AGG - Chr2:42217321-42217340 MsG0280008954.01.T01:intron 40.0%
!! GTTAAGCGTATCGATTTAGG+TGG - Chr2:42217324-42217343 MsG0280008954.01.T01:intron 40.0%
!!! GGCAGCATTATTTTAACTGG+TGG - Chr2:42218396-42218415 MsG0280008954.01.T01:CDS 40.0%
AACCCCTCACCTCCAAAAAA+TGG - Chr2:42216875-42216894 MsG0280008954.01.T01:intron 45.0%
AATATCCGGAACAAGCAACC+AGG - Chr2:42217565-42217584 MsG0280008954.01.T01:CDS 45.0%
AATGGAAGGGAGAGTGAAGA+GGG + Chr2:42217097-42217116 None:intergenic 45.0%
ACATACCCAAATCAGCTGGA+CGG + Chr2:42217830-42217849 None:intergenic 45.0%
ACTGCTCGTACAATGCATTC+TGG + Chr2:42218227-42218246 None:intergenic 45.0%
ACTTTCTGTCTGTTGTCCTG+AGG + Chr2:42217657-42217676 None:intergenic 45.0%
AGATACCTGGTTGCTTGTTC+CGG + Chr2:42217573-42217592 None:intergenic 45.0%
AGGGGTTTGTAGAGACAGTT+GGG + Chr2:42216862-42216881 None:intergenic 45.0%
ATTAAGTAGCCGCGATCGAT+GGG + Chr2:42216982-42217001 None:intergenic 45.0%
CGAGGGTGAGAAGTAAGAAA+AGG + Chr2:42218349-42218368 None:intergenic 45.0%
GGTTTCGTAGAGGTGAACAA+GGG + Chr2:42217190-42217209 None:intergenic 45.0%
TACAATCTCTCGGCAAAGTG+AGG + Chr2:42218435-42218454 None:intergenic 45.0%
TGAATCAGCGACGTAAAGAG+AGG + Chr2:42217163-42217182 None:intergenic 45.0%
TGGTTTCGTAGAGGTGAACA+AGG + Chr2:42217191-42217210 None:intergenic 45.0%
TTTGTGTGCGTAACACCATC+AGG + Chr2:42217534-42217553 None:intergenic 45.0%
! AATCTTCGAAGACGAGTTGG+TGG + Chr2:42217131-42217150 None:intergenic 45.0%
! ATCTTCCGTCCAGCTGATTT+GGG - Chr2:42217822-42217841 MsG0280008954.01.T01:CDS 45.0%
! CAGGTGGTTGTTCTAGACAA+CGG - Chr2:42216781-42216800 MsG0280008954.01.T01:CDS 45.0%
! CAGTTGGGGAGTATAGTTGA+TGG + Chr2:42216847-42216866 None:intergenic 45.0%
! CTAATAAAAGCCGGTTTCGG+CGG - Chr2:42216811-42216830 MsG0280008954.01.T01:intron 45.0%
!! AAGGGGTGTTGATAGGGTTT+GGG + Chr2:42216906-42216925 None:intergenic 45.0%
!! AGGATTGAGAAGGGTTGATG+CGG + Chr2:42217056-42217075 None:intergenic 45.0%
!! GATGGTGTTACGCACACAAA+AGG - Chr2:42217534-42217553 MsG0280008954.01.T01:CDS 45.0%
!! GCTTTAGAGAGAAGGCCATT+GGG + Chr2:42217222-42217241 None:intergenic 45.0%
!! TGCAGTCTCGTTGTTGATTC+TGG - Chr2:42217246-42217265 MsG0280008954.01.T01:intron 45.0%
!! TGCATTGGGCTTTAGAGAGA+AGG + Chr2:42217230-42217249 None:intergenic 45.0%
!!! AGCCATTTTTTGGAGGTGAG+GGG + Chr2:42216880-42216899 None:intergenic 45.0%
!!! GAGCCATTTTTTGGAGGTGA+GGG + Chr2:42216881-42216900 None:intergenic 45.0%
!!! GTTTGGGTGGAGCCATTTTT+TGG + Chr2:42216890-42216909 None:intergenic 45.0%
AGCTGGACGGAAGATCATCT+CGG + Chr2:42217817-42217836 None:intergenic 50.0%
AGGAGGAGAGAGGATTGAGA+AGG + Chr2:42217066-42217085 None:intergenic 50.0%
AGGATGAAGATGCGGATGAC+AGG + Chr2:42218255-42218274 None:intergenic 50.0%
ATGGAAGGGAGAGTGAAGAG+GGG + Chr2:42217096-42217115 None:intergenic 50.0%
CAACATGTCATCAAGCACGC+AGG - Chr2:42216762-42216781 MsG0280008954.01.T01:CDS 50.0%
CTCGGCAAAGTGAGGAAACA+AGG + Chr2:42218427-42218446 None:intergenic 50.0%
GAATGGAAGGGAGAGTGAAG+AGG + Chr2:42217098-42217117 None:intergenic 50.0%
GAGGATAGAGGTGAAGAGGT+GGG + Chr2:42217034-42217053 None:intergenic 50.0%
GAGGGGTTTGTAGAGACAGT+TGG + Chr2:42216863-42216882 None:intergenic 50.0%
GATCTTCCGTCCAGCTGATT+TGG - Chr2:42217821-42217840 MsG0280008954.01.T01:CDS 50.0%
GATTAAGTAGCCGCGATCGA+TGG + Chr2:42216983-42217002 None:intergenic 50.0%
GCAACCAGGTATCTTGCTGA+TGG - Chr2:42217579-42217598 MsG0280008954.01.T01:CDS 50.0%
GGAGGAGAGAGGATTGAGAA+GGG + Chr2:42217065-42217084 None:intergenic 50.0%
GGCGGAGGACTAATAAAAGC+CGG - Chr2:42216802-42216821 MsG0280008954.01.T01:intron 50.0%
GGGGTTTGTAGAGACAGTTG+GGG + Chr2:42216861-42216880 None:intergenic 50.0%
TCGTAGAGGTGAACAAGGGA+AGG + Chr2:42217186-42217205 None:intergenic 50.0%
TTAAGTAGCCGCGATCGATG+GGG + Chr2:42216981-42217000 None:intergenic 50.0%
TTGCAGGAATGAACCAGGCT+GGG - Chr2:42218198-42218217 MsG0280008954.01.T01:CDS 50.0%
TTTGCAGGAATGAACCAGGC+TGG - Chr2:42218197-42218216 MsG0280008954.01.T01:CDS 50.0%
! GTGGTTGTTCTAGACAACGG+CGG - Chr2:42216784-42216803 MsG0280008954.01.T01:CDS 50.0%
!! AGGAGGAAGGGGTGTTGATA+GGG + Chr2:42216912-42216931 None:intergenic 50.0%
!! ATTGAGAAGGGTTGATGCGG+AGG + Chr2:42217053-42217072 None:intergenic 50.0%
!! GAAGGGGTGTTGATAGGGTT+TGG + Chr2:42216907-42216926 None:intergenic 50.0%
!! GGAGGGAGTGTTGAATGGAA+GGG + Chr2:42217110-42217129 None:intergenic 50.0%
!! GGCTTTAGAGAGAAGGCCAT+TGG + Chr2:42217223-42217242 None:intergenic 50.0%
!! TGGAGGGAGTGTTGAATGGA+AGG + Chr2:42217111-42217130 None:intergenic 50.0%
!! TTCGAAGACGAGTTGGTGGA+GGG + Chr2:42217127-42217146 None:intergenic 50.0%
!! TTGGTGGAGGGAGTGTTGAA+TGG + Chr2:42217115-42217134 None:intergenic 50.0%
!!! GGAGCCATTTTTTGGAGGTG+AGG + Chr2:42216882-42216901 None:intergenic 50.0%
!!! TGGGTGGAGCCATTTTTTGG+AGG + Chr2:42216887-42216906 None:intergenic 50.0%
AGAGAAGGCCATTGGGACGT+TGG + Chr2:42217215-42217234 None:intergenic 55.0%
AGATATCGCGTTGGAGGTCG+GGG + Chr2:42217008-42217027 None:intergenic 55.0%
CAGATATCGCGTTGGAGGTC+GGG + Chr2:42217009-42217028 None:intergenic 55.0%
CATGTCATCAAGCACGCAGG+TGG - Chr2:42216765-42216784 MsG0280008954.01.T01:CDS 55.0%
CCAGATATCGCGTTGGAGGT+CGG + Chr2:42217010-42217029 None:intergenic 55.0%
CGAATGGGGAAGATGTGTCG+CGG - Chr2:42217961-42217980 MsG0280008954.01.T01:CDS 55.0%
CTGGACGGAAGATCATCTCG+GGG + Chr2:42217815-42217834 None:intergenic 55.0%
GAAACCAGCCAACGTCCCAA+TGG - Chr2:42217204-42217223 MsG0280008954.01.T01:intron 55.0%
GAGTGAAGAGGGGTTGAGTG+AGG + Chr2:42217086-42217105 None:intergenic 55.0%
GCTGGACGGAAGATCATCTC+GGG + Chr2:42217816-42217835 None:intergenic 55.0%
GGACGATATCGGTGATCACG+AGG - Chr2:42217614-42217633 MsG0280008954.01.T01:CDS 55.0%
GGAGGATAGAGGTGAAGAGG+TGG + Chr2:42217035-42217054 None:intergenic 55.0%
TGAAGAGGGGTTGAGTGAGG+AGG + Chr2:42217083-42217102 None:intergenic 55.0%
TGCGGAGGATAGAGGTGAAG+AGG + Chr2:42217038-42217057 None:intergenic 55.0%
! GTTGTTCTAGACAACGGCGG+AGG - Chr2:42216787-42216806 MsG0280008954.01.T01:intron 55.0%
!! AGGGTTGATGCGGAGGATAG+AGG + Chr2:42217046-42217065 None:intergenic 55.0%
!! CTTCGAAGACGAGTTGGTGG+AGG + Chr2:42217128-42217147 None:intergenic 55.0%
!! GACGTTGGCTGGTTTCGTAG+AGG + Chr2:42217200-42217219 None:intergenic 55.0%
!! GAGGAGGAAGGGGTGTTGAT+AGG + Chr2:42216913-42216932 None:intergenic 55.0%
!! GGGTGTTGATAGGGTTTGGG+TGG + Chr2:42216903-42216922 None:intergenic 55.0%
AAGGCCATTGGGACGTTGGC+TGG + Chr2:42217211-42217230 None:intergenic 60.0%
AGGAGGAGGAGGAGGAGGAA+GGG + Chr2:42216924-42216943 None:intergenic 60.0%
CCGACCTCCAACGCGATATC+TGG - Chr2:42217007-42217026 MsG0280008954.01.T01:intron 60.0%
GACAGGCGAAGCGTGTGTGA+AGG + Chr2:42217277-42217296 None:intergenic 60.0%
GGGACCAGATATCGCGTTGG+AGG + Chr2:42217014-42217033 None:intergenic 60.0%
GGGTTGAGTGAGGAGGAGAG+AGG + Chr2:42217076-42217095 None:intergenic 60.0%
GGTGGGACCAGATATCGCGT+TGG + Chr2:42217017-42217036 None:intergenic 60.0%
TGCATTCTGGTAGCCCAGCC+TGG + Chr2:42218214-42218233 None:intergenic 60.0%
TTGTTCGGAGGAGGAGGAGG+AGG + Chr2:42216932-42216951 None:intergenic 60.0%
! GTCTTGTTCGGAGGAGGAGG+AGG + Chr2:42216935-42216954 None:intergenic 60.0%
!! GAGGTCTTGTTCGGAGGAGG+AGG + Chr2:42216938-42216957 None:intergenic 60.0%
!! GGAGGTGAGGTCTTGTTCGG+AGG + Chr2:42216944-42216963 None:intergenic 60.0%
!! GGCGGAGGTGAGGTCTTGTT+CGG + Chr2:42216947-42216966 None:intergenic 60.0%
!! GGTGAGGTCTTGTTCGGAGG+AGG + Chr2:42216941-42216960 None:intergenic 60.0%
AGTAGCCGCGATCGATGGGG+CGG + Chr2:42216978-42216997 None:intergenic 65.0%
GAGGAGGAGGAGGAGGAGGA+AGG + Chr2:42216925-42216944 None:intergenic 65.0%
GGAGGAGGAGGAGGAGGAAG+GGG + Chr2:42216923-42216942 None:intergenic 65.0%
TTCGGAGGAGGAGGAGGAGG+AGG + Chr2:42216929-42216948 None:intergenic 65.0%
ATCGATGGGGCGGCGAACGG+AGG + Chr2:42216968-42216987 None:intergenic 70.0%
GCGATCGATGGGGCGGCGAA+CGG + Chr2:42216971-42216990 None:intergenic 70.0%
GTCGCGTTCGCCGCCGAAAC+CGG + Chr2:42216824-42216843 None:intergenic 70.0%
GTTCGCCGCCCCATCGATCG+CGG - Chr2:42216970-42216989 MsG0280008954.01.T01:CDS 70.0%
GATGGGGCGGCGAACGGAGG+CGG + Chr2:42216965-42216984 None:intergenic 75.0%
GGCGAACGGAGGCGGAGGTG+AGG + Chr2:42216957-42216976 None:intergenic 75.0%
! GGGGCGGCGAACGGAGGCGG+AGG + Chr2:42216962-42216981 None:intergenic 85.0%
Chromosome Type Strat End Strand Name
Chr2 gene 42216750 42218455 42216750 ID=MsG0280008954.01;Name=MsG0280008954.01
Chr2 mRNA 42216750 42218455 42216750 ID=MsG0280008954.01.T01;Parent=MsG0280008954.01;Name=MsG0280008954.01.T01;_AED=0.34;_eAED=0.34;_QI=16|1|0.75|1|1|1|4|0|376
Chr2 exon 42216750 42216804 42216750 ID=MsG0280008954.01.T01:exon:26257;Parent=MsG0280008954.01.T01
Chr2 exon 42216917 42217001 42216917 ID=MsG0280008954.01.T01:exon:26256;Parent=MsG0280008954.01.T01
Chr2 exon 42217362 42217425 42217362 ID=MsG0280008954.01.T01:exon:26255;Parent=MsG0280008954.01.T01
Chr2 exon 42217513 42218455 42217513 ID=MsG0280008954.01.T01:exon:26254;Parent=MsG0280008954.01.T01
Chr2 five_prime_UTR 42218440 42218455 42218440 ID=MsG0280008954.01.T01:five_prime_utr;Parent=MsG0280008954.01.T01
Chr2 CDS 42217513 42218439 42217513 ID=MsG0280008954.01.T01:cds;Parent=MsG0280008954.01.T01
Chr2 CDS 42217362 42217425 42217362 ID=MsG0280008954.01.T01:cds;Parent=MsG0280008954.01.T01
Chr2 CDS 42216917 42217001 42216917 ID=MsG0280008954.01.T01:cds;Parent=MsG0280008954.01.T01
Chr2 CDS 42216750 42216804 42216750 ID=MsG0280008954.01.T01:cds;Parent=MsG0280008954.01.T01
Gene Sequence

>MsG0280008954.01.T01

ATGTCATCAAGCACGCAGGTGGTTGTTCTAGACAACGGCGGAGGACTAATAAAAGCCGGTTTCGGCGGCGAACGCGACCCATCAACTATACTCCCCAACTGTCTCTACAAACCCCTCACCTCCAAAAAATGGCTCCACCCAAACCCTATCAACACCCCTTCCTCCTCCTCCTCCTCCTCCGAACAAGACCTCACCTCCGCCTCCGTTCGCCGCCCCATCGATCGCGGCTACTTAATCAACCCCGACCTCCAACGCGATATCTGGTCCCACCTCTTCACCTCTATCCTCCGCATCAACCCTTCTCAATCCTCTCTCCTCCTCACTCAACCCCTCTTCACTCTCCCTTCCATTCAACACTCCCTCCACCAACTCGTCTTCGAAGATTTCAACTTCTCCTCTCTTTACGTCGCTGATTCACCTTCCCTTGTTCACCTCTACGAAACCAGCCAACGTCCCAATGGCCTTCTCTCTAAAGCCCAATGCAGTCTCGTTGTTGATTCTGGCTTTTCCTTCACACACGCTTCGCCTGTCTTTCAGAATTTTACTCTTAACTACGCTGTTAAGCGTATCGATTTAGGTGGTAAAGCCTTGACTAATTACCTCAAGGAACTTATTTCCTTTCGTTCTGTTAATGTCATGGATGAAACTTTTATTATTGATGATGTTAAGGAGAATCTCTGCTTTGTTTCTGTTGATGTCGACAAACACCTTAACTTTGCTAGAAAGAGTGGGAAAGAGAATGTTTTCAAGTGTACGTATGTTCTTCCTGATGGTGTTACGCACACAAAAGGGTTTGTTAAATATCCGGAACAAGCAACCAGGTATCTTGCTGATGGTAAAGGAATGAAGGACGATATCGGTGATCACGAGGAGATTTCTAAACAACAACCTCAGGACAACAGACAGAAAGTTGATTTGACAAAAAATGAATTTGATTTGACGAATGAACGGTTTCTTGTCCCCGAGATGATCTTCCGTCCAGCTGATTTGGGAATGAACCAGGCTGGGCTACCAGAATGCATTGTACGAGCAGTTAATGCCTGTCATCCGCATCTTCATCCTGTACTCTATGAAAGCATTATTTTAACTGGTGGAAGCACCTTGTTTCCTCACTTTGCCGAGAGATTGTAA
Protein sequence

>MsG0280008954.01.T01

MSSSTQVVVLDNGGGLIKAGFGGERDPSTILPNCLYKPLTSKKWLHPNPINTPSSSSSSSEQDLTSASVRRPIDRGYLINPDLQRDIWSHLFTSILRINPSQSSLLLTQPLFTLPSIQHSLHQLVFEDFNFSSLYVADSPSLVHLYETSQRPNGLLSKAQCSLVVDSGFSFTHASPVFQNFTLNYAVKRIDLGGKALTNYLKELISFRSVNVMDETFIIDDVKENLCFVSVDVDKHLNFARKSGKENVFKCTYVLPDGVTHTKGFVKYPEQATRYLADGKGMKDDIGDHEEISKQQPQDNRQKVDLTKNEFDLTNERFLVPEMIFRPADLGMNQAGLPECIVRAVNACHPHLHPVLYESIILTGGSTLFPHFAERL*