AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar ZhongmuNo.1 / MsG0280009026.01

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MsG0280009026.01.T01 MTR_2g450740 96.899 129 3 1 1 128 1 129 5.95e-86 256
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MsG0280009026.01.T01 AT5G35080 50.394 127 54 2 3 128 7 125 2.00e-34 123

Find 50 sgRNAs with CRISPR-Local

Find 223 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
ATTTGATTAGGTAGTATATT+TGG 0.272726 2:-43684184 MsG0280009026.01.T01:intron
GAAATTTCGACAACTGCATT+TGG 0.318537 2:-43682847 MsG0280009026.01.T01:CDS
ATCTCTATATTTACTTTGAA+TGG 0.327493 2:-43683010 MsG0280009026.01.T01:CDS
TGAAATTCAATCTTGTATTT+CGG 0.348436 2:+43684121 None:intergenic
GGGTGAAAAGGGGAATCTTC+TGG 0.350449 2:+43684097 None:intergenic
AAAGAATTACCGATATGAAC+AGG 0.357031 2:+43684329 None:intergenic
CCGGTACAACTAAGGATTCC+TGG 0.381467 2:+43683218 None:intergenic
AATATTTCTAATAGTTCATC+TGG 0.388576 2:+43683067 None:intergenic
CATAGCTTACAGGGTGAAAA+GGG 0.391297 2:+43684086 None:intergenic
AATTTCTGTCCATTCTTATC+CGG 0.403358 2:+43683199 None:intergenic
ACATAGCTTACAGGGTGAAA+AGG 0.416080 2:+43684085 None:intergenic
CAAATCTTCCCTGTTCATAT+CGG 0.432213 2:-43684338 MsG0280009026.01.T01:intron
GGTAGTATATTTGGTGGTAG+TGG 0.438045 2:-43684175 MsG0280009026.01.T01:CDS
ATTCTTATCCGGTACAACTA+AGG 0.453860 2:+43683210 None:intergenic
TTACTTGCCTAAGGTGGAAA+AGG 0.458543 2:-43683170 MsG0280009026.01.T01:CDS
GATTGTTGAAACTGAGAAAA+GGG 0.464813 2:-43683104 MsG0280009026.01.T01:CDS
GACCTATGGCCTTCCAACCT+TGG 0.474255 2:-43681622 MsG0280009026.01.T01:CDS
TGATTGTTGAAACTGAGAAA+AGG 0.478392 2:-43683105 MsG0280009026.01.T01:CDS
AGTGGTGGTGGTGGTAGTAG+TGG 0.484864 2:-43684157 MsG0280009026.01.T01:CDS
CTAACATTGTGCTGCATAGG+AGG 0.485706 2:+43683133 None:intergenic
CCATGCCATGCCTCCAAGGT+TGG 0.488416 2:+43681609 None:intergenic
ATATTTGGTGGTAGTGGTGG+TGG 0.489525 2:-43684169 MsG0280009026.01.T01:CDS
GCCTTCCAACCTTGGAGGCA+TGG 0.495827 2:-43681614 MsG0280009026.01.T01:CDS
ATTTATATGTTACTTGCCTA+AGG 0.501089 2:-43683179 MsG0280009026.01.T01:CDS
CTCGTTTGTGTTAAGTTGTG+AGG 0.503479 2:-43684439 MsG0280009026.01.T01:five_prime_UTR
AGTATATTTGGTGGTAGTGG+TGG 0.518758 2:-43684172 MsG0280009026.01.T01:CDS
CCATGTCGCCTAGTCACAAA+AGG 0.523860 2:-43681574 MsG0280009026.01.T01:CDS
TGAACTATTAGAAATATTGA+AGG 0.525549 2:-43683062 MsG0280009026.01.T01:CDS
CCAGGAATCCTTAGTTGTAC+CGG 0.525699 2:-43683218 MsG0280009026.01.T01:CDS
TCGCCTAGTCACAAAAGGTA+TGG 0.527039 2:-43681569 MsG0280009026.01.T01:CDS
CTATGGCCTTCCAACCTTGG+AGG 0.529295 2:-43681619 MsG0280009026.01.T01:CDS
TTTGGTGGTAGTGGTGGTGG+TGG 0.533771 2:-43684166 MsG0280009026.01.T01:CDS
CCATTAGATTCACTGACCTA+TGG 0.533878 2:-43681636 MsG0280009026.01.T01:intron
GCCATGCCTCCAAGGTTGGA+AGG 0.543979 2:+43681613 None:intergenic
TTAGTTGTACCGGATAAGAA+TGG 0.545277 2:-43683208 MsG0280009026.01.T01:CDS
ATTTCGACAACTGCATTTGG+AGG 0.546289 2:-43682844 MsG0280009026.01.T01:CDS
TATATGTTACTTGCCTAAGG+TGG 0.561139 2:-43683176 MsG0280009026.01.T01:CDS
CATGCCATGCCATGCCTCCA+AGG 0.561487 2:+43681605 None:intergenic
ATACTAACTCTGATAAAGCA+TGG 0.564281 2:+43683037 None:intergenic
TGATTAGGTAGTATATTTGG+TGG 0.566982 2:-43684181 MsG0280009026.01.T01:intron
CTCCAAGGTTGGAAGGCCAT+AGG 0.578297 2:+43681620 None:intergenic
GATTTGTTCAGCAAAGACAT+AGG 0.582467 2:+43684355 None:intergenic
TTGCTAACATTGTGCTGCAT+AGG 0.596398 2:+43683130 None:intergenic
CCAACCTTGGAGGCATGGCA+TGG 0.611494 2:-43681609 MsG0280009026.01.T01:CDS
CTAGTCACAAAAGGTATGGA+TGG 0.615929 2:-43681565 MsG0280009026.01.T01:CDS
AAGAATTACCGATATGAACA+GGG 0.633857 2:+43684330 None:intergenic
GAACTATTAGAAATATTGAA+GGG 0.635775 2:-43683061 MsG0280009026.01.T01:CDS
ACTGCATTTGGAGGAAGACA+AGG 0.659258 2:-43682835 MsG0280009026.01.T01:intron
AAATTTACAGGATAATGACC+AGG 0.668049 2:-43683236 MsG0280009026.01.T01:intron
ATAGCTTACAGGGTGAAAAG+GGG 0.693438 2:+43684087 None:intergenic

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!! TTTATAAAAAAGATAATCAA+GGG + Chr2:43683279-43683298 None:intergenic 10.0%
!! ATAAAAAATTGAAAACCTTA+TGG + Chr2:43681833-43681852 None:intergenic 15.0%
!! GTTTATAAAAAAGATAATCA+AGG + Chr2:43683280-43683299 None:intergenic 15.0%
!!! ATTTCAATTTTGTCAAATAA+TGG + Chr2:43682541-43682560 None:intergenic 15.0%
!!! TACAAAAGTTTAAATGTTTT+AGG + Chr2:43683481-43683500 None:intergenic 15.0%
!!! TGTATGATATTAATTTGATT+AGG - Chr2:43681866-43681885 MsG0280009026.01.T01:intron 15.0%
!! AAGTTTATAAACAGATAGTT+TGG - Chr2:43683096-43683115 MsG0280009026.01.T01:CDS 20.0%
!! ACAAGAAAAGAAAAAAAATG+AGG - Chr2:43681650-43681669 MsG0280009026.01.T01:intron 20.0%
!! ATCTCTATATTTACTTTGAA+TGG - Chr2:43683052-43683071 MsG0280009026.01.T01:CDS 20.0%
!! ATTTGATTAGGTAGTATATT+TGG - Chr2:43681878-43681897 MsG0280009026.01.T01:intron 20.0%
!! GAACTATTAGAAATATTGAA+GGG - Chr2:43683001-43683020 MsG0280009026.01.T01:intron 20.0%
!! TGAAATTCAATCTTGTATTT+CGG + Chr2:43681944-43681963 None:intergenic 20.0%
!! TGAACTATTAGAAATATTGA+AGG - Chr2:43683000-43683019 MsG0280009026.01.T01:intron 20.0%
!! TGATTAATATTCTATCCATA+AGG - Chr2:43681815-43681834 MsG0280009026.01.T01:intron 20.0%
!!! AATATTTCTAATAGTTCATC+TGG + Chr2:43682998-43683017 None:intergenic 20.0%
! AAAACAATACATAGCTTACA+GGG + Chr2:43681988-43682007 None:intergenic 25.0%
! AAGAACTGTAACAACAAAAT+TGG + Chr2:43683783-43683802 None:intergenic 25.0%
! CTTTCCAAGATGTATTTATA+CGG + Chr2:43683904-43683923 None:intergenic 25.0%
! TAAAACAATACATAGCTTAC+AGG + Chr2:43681989-43682008 None:intergenic 25.0%
! TATGTCTATAAATGGGTAAA+AGG + Chr2:43683643-43683662 None:intergenic 25.0%
! TGTAAATCACTTTCTTACAT+TGG - Chr2:43683136-43683155 MsG0280009026.01.T01:CDS 25.0%
!! AATTGTGTTGCTAAATTTAC+AGG - Chr2:43682814-43682833 MsG0280009026.01.T01:intron 25.0%
!! ATTTATATGTTACTTGCCTA+AGG - Chr2:43682883-43682902 MsG0280009026.01.T01:CDS 25.0%
AAAGAATTACCGATATGAAC+AGG + Chr2:43681736-43681755 None:intergenic 30.0%
AAATTTACAGGATAATGACC+AGG - Chr2:43682826-43682845 MsG0280009026.01.T01:intron 30.0%
AAGAATTACCGATATGAACA+GGG + Chr2:43681735-43681754 None:intergenic 30.0%
AATTTCTGTCCATTCTTATC+CGG + Chr2:43682866-43682885 None:intergenic 30.0%
ACAAGTGCCAAATATTTGTT+TGG + Chr2:43684360-43684379 None:intergenic 30.0%
ACAGTTCTTACAAAGCTTTA+AGG - Chr2:43683794-43683813 MsG0280009026.01.T01:intron 30.0%
ATAAATACATCTTGGAAAGC+AGG - Chr2:43683905-43683924 MsG0280009026.01.T01:intron 30.0%
ATACTAACTCTGATAAAGCA+TGG + Chr2:43683028-43683047 None:intergenic 30.0%
ATCCAGTTACAACTTTGTAT+GGG - Chr2:43683585-43683604 MsG0280009026.01.T01:intron 30.0%
ATTTACAACAAGACTTCTGA+GGG + Chr2:43683123-43683142 None:intergenic 30.0%
CAACACAATTTCTTATCAGA+AGG + Chr2:43682804-43682823 None:intergenic 30.0%
CAAGTGCCAAATATTTGTTT+GGG + Chr2:43684359-43684378 None:intergenic 30.0%
CTGTAACAACAAAATTGGAT+TGG + Chr2:43683778-43683797 None:intergenic 30.0%
GATTGTTGAAACTGAGAAAA+GGG - Chr2:43682958-43682977 MsG0280009026.01.T01:intron 30.0%
TAAACTAAGCGATTAAACAG+AGG + Chr2:43681762-43681781 None:intergenic 30.0%
TAAATACATCTTGGAAAGCA+GGG - Chr2:43683906-43683925 MsG0280009026.01.T01:intron 30.0%
TACCCATTTATAGACATAGA+GGG - Chr2:43683645-43683664 MsG0280009026.01.T01:intron 30.0%
TATACAAAACATTTCAACCG+TGG + Chr2:43682214-43682233 None:intergenic 30.0%
TATTTGGCACTTGTTTAGAT+TGG - Chr2:43684366-43684385 MsG0280009026.01.T01:CDS 30.0%
TCTATAAGTGTTAACGATTG+CGG - Chr2:43682398-43682417 MsG0280009026.01.T01:intron 30.0%
TCTCACAAATCCATTACATT+GGG + Chr2:43683332-43683351 None:intergenic 30.0%
TGATTAGGTAGTATATTTGG+TGG - Chr2:43681881-43681900 MsG0280009026.01.T01:intron 30.0%
TGATTGTTGAAACTGAGAAA+AGG - Chr2:43682957-43682976 MsG0280009026.01.T01:intron 30.0%
TGGTTAGGAATCAAAATGAT+GGG - Chr2:43682246-43682265 MsG0280009026.01.T01:intron 30.0%
TTACCCATTTATAGACATAG+AGG - Chr2:43683644-43683663 MsG0280009026.01.T01:intron 30.0%
TTAGGACAAAAATGTGATGT+TGG - Chr2:43682129-43682148 MsG0280009026.01.T01:intron 30.0%
TTGAATGTAACCACATATGT+CGG + Chr2:43682616-43682635 None:intergenic 30.0%
TTGATGTTATCAGAGAGATT+GGG - Chr2:43682288-43682307 MsG0280009026.01.T01:intron 30.0%
TTGATTCTTGAATGTTACCA+CGG - Chr2:43682194-43682213 MsG0280009026.01.T01:intron 30.0%
TTTGCCGTATAAATACATCT+TGG - Chr2:43683897-43683916 MsG0280009026.01.T01:intron 30.0%
TTTGTTTCATCATCATGGAT+GGG - Chr2:43683990-43684009 MsG0280009026.01.T01:intron 30.0%
! ACAGCTTAATTTAAAACGCT+AGG + Chr2:43681789-43681808 None:intergenic 30.0%
! ACGGTTGAAATGTTTTGTAT+AGG - Chr2:43682213-43682232 MsG0280009026.01.T01:intron 30.0%
! AGAATTTTATGACAACTGTC+AGG + Chr2:43682499-43682518 None:intergenic 30.0%
! CGGTTGAAATGTTTTGTATA+GGG - Chr2:43682214-43682233 MsG0280009026.01.T01:intron 30.0%
! GAATTTTATGACAACTGTCA+GGG + Chr2:43682498-43682517 None:intergenic 30.0%
! GGGCATTTTTGTAAAATTTG+TGG - Chr2:43683665-43683684 MsG0280009026.01.T01:intron 30.0%
! TTTTTGTCCTAAACTGTATG+AGG + Chr2:43682121-43682140 None:intergenic 30.0%
AAATATTTGTTTGGGTGCAG+TGG + Chr2:43684351-43684370 None:intergenic 35.0%
AAATCCCCTATCTCACAAAA+AGG + Chr2:43682332-43682351 None:intergenic 35.0%
AAGGTGGAAAAGGAAAAAAG+TGG - Chr2:43682902-43682921 MsG0280009026.01.T01:intron 35.0%
ACAGATTTCCATGTTCTCTA+AGG + Chr2:43683858-43683877 None:intergenic 35.0%
ACTGCACCCAAACAAATATT+TGG - Chr2:43684350-43684369 MsG0280009026.01.T01:CDS 35.0%
ATCGTTGGTCAATTCATCTA+GGG - Chr2:43682075-43682094 MsG0280009026.01.T01:intron 35.0%
ATGCCCTCTATGTCTATAAA+TGG + Chr2:43683651-43683670 None:intergenic 35.0%
ATGGGCTTCAATCATTTGTA+CGG - Chr2:43683603-43683622 MsG0280009026.01.T01:intron 35.0%
ATTCTTATCCGGTACAACTA+AGG + Chr2:43682855-43682874 None:intergenic 35.0%
CAAAATTGCAAACACATCGT+TGG - Chr2:43682060-43682079 MsG0280009026.01.T01:intron 35.0%
CAAATCTTCCCTGTTCATAT+CGG - Chr2:43681724-43681743 MsG0280009026.01.T01:intron 35.0%
CAGATTTCCATGTTCTCTAA+GGG + Chr2:43683857-43683876 None:intergenic 35.0%
CTAATGGTCAGATTCATTGA+AGG + Chr2:43684413-43684432 None:intergenic 35.0%
CTCTCACAAATCCATTACAT+TGG + Chr2:43683333-43683352 None:intergenic 35.0%
CTGGTTAGGAATCAAAATGA+TGG - Chr2:43682245-43682264 MsG0280009026.01.T01:intron 35.0%
GAAATTTCGACAACTGCATT+TGG - Chr2:43683215-43683234 MsG0280009026.01.T01:CDS 35.0%
GAACACTGGAATATTCTAAG+AGG + Chr2:43684286-43684305 None:intergenic 35.0%
GATCCAGTTACAACTTTGTA+TGG - Chr2:43683584-43683603 MsG0280009026.01.T01:intron 35.0%
GATTTACAACAAGACTTCTG+AGG + Chr2:43683124-43683143 None:intergenic 35.0%
GCAAAAACATTTGCACTCTA+AGG + Chr2:43683882-43683901 None:intergenic 35.0%
GCATCATAAAACTGCTGTAA+GGG - Chr2:43683562-43683581 MsG0280009026.01.T01:intron 35.0%
GTCTATGTTATGAAGTAACC+CGG - Chr2:43684022-43684041 MsG0280009026.01.T01:intron 35.0%
GTTGATGTTATCAGAGAGAT+TGG - Chr2:43682287-43682306 MsG0280009026.01.T01:intron 35.0%
GTTTCTCTTTCCAAGTCTAA+TGG - Chr2:43683524-43683543 MsG0280009026.01.T01:intron 35.0%
GTTTGTTTCATCATCATGGA+TGG - Chr2:43683989-43684008 MsG0280009026.01.T01:intron 35.0%
TAAAACGCTCATCTTTGCTA+AGG - Chr2:43684309-43684328 MsG0280009026.01.T01:intron 35.0%
TGCATCATAAAACTGCTGTA+AGG - Chr2:43683561-43683580 MsG0280009026.01.T01:intron 35.0%
TGCCCTCTATGTCTATAAAT+GGG + Chr2:43683650-43683669 None:intergenic 35.0%
TTAGTTGTACCGGATAAGAA+TGG - Chr2:43682854-43682873 MsG0280009026.01.T01:CDS 35.0%
TTGTCAAATAATGGCCAATG+TGG + Chr2:43682532-43682551 None:intergenic 35.0%
! AGAAATGTTCACTGACTGTA+AGG - Chr2:43682362-43682381 MsG0280009026.01.T01:intron 35.0%
! ATTGCACTCGTCTAAAGTTT+AGG + Chr2:43684125-43684144 None:intergenic 35.0%
! GATTTGTTCAGCAAAGACAT+AGG + Chr2:43681710-43681729 None:intergenic 35.0%
! TATATGTTACTTGCCTAAGG+TGG - Chr2:43682886-43682905 MsG0280009026.01.T01:CDS 35.0%
! TTCGTCCTTTTTGTGAGATA+GGG - Chr2:43682324-43682343 MsG0280009026.01.T01:intron 35.0%
! TTGCACTCGTCTAAAGTTTA+GGG + Chr2:43684124-43684143 None:intergenic 35.0%
! TTTCGTCCTTTTTGTGAGAT+AGG - Chr2:43682323-43682342 MsG0280009026.01.T01:intron 35.0%
! TTTGTATAGGGTATCTATGC+TGG - Chr2:43682226-43682245 MsG0280009026.01.T01:intron 35.0%
!! AATTGGATTGGTCTTCTACT+CGG + Chr2:43683766-43683785 None:intergenic 35.0%
!! CAATGTAATGGATTTGTGAG+AGG - Chr2:43683331-43683350 MsG0280009026.01.T01:intron 35.0%
!! TCTTACAAAGCTTTAAGGCT+AGG - Chr2:43683799-43683818 MsG0280009026.01.T01:intron 35.0%
!! TGAGCGTTTTATTAGAACAC+TGG + Chr2:43684300-43684319 None:intergenic 35.0%
!!! AGCGACACTATGTCATTTTA+CGG + Chr2:43682459-43682478 None:intergenic 35.0%
!!! AGCTTCTTGTTTTGACTAGA+AGG - Chr2:43684215-43684234 MsG0280009026.01.T01:intron 35.0%
!!! CTTTTTTCCTTTTCCACCTT+AGG + Chr2:43682902-43682921 None:intergenic 35.0%
AATCCTCACACTTCACTGTA+TGG + Chr2:43683934-43683953 None:intergenic 40.0%
AATGACGTAGTCACAGATAC+TGG + Chr2:43682690-43682709 None:intergenic 40.0%
AATGTTAATCTCGGAGCTGA+GGG - Chr2:43683967-43683986 MsG0280009026.01.T01:intron 40.0%
AATTTGTGGACACCATCACA+AGG - Chr2:43683679-43683698 MsG0280009026.01.T01:intron 40.0%
ACATAGCTTACAGGGTGAAA+AGG + Chr2:43681980-43681999 None:intergenic 40.0%
AGAGAGATTGGGAATAAGAG+TGG - Chr2:43682299-43682318 MsG0280009026.01.T01:intron 40.0%
AGATATTCATCCCACCGAAA+AGG + Chr2:43682770-43682789 None:intergenic 40.0%
AGCCCATACAAAGTTGTAAC+TGG + Chr2:43683590-43683609 None:intergenic 40.0%
AGCCTCCTCAATGTTAATCT+CGG - Chr2:43683958-43683977 MsG0280009026.01.T01:intron 40.0%
ATAGCTTACAGGGTGAAAAG+GGG + Chr2:43681978-43681997 None:intergenic 40.0%
ATCTAGGGACTATAGTGTAG+TGG - Chr2:43682090-43682109 MsG0280009026.01.T01:intron 40.0%
ATGTTATGAAGTAACCCGGT+AGG - Chr2:43684026-43684045 MsG0280009026.01.T01:intron 40.0%
ATTTCGACAACTGCATTTGG+AGG - Chr2:43683218-43683237 MsG0280009026.01.T01:CDS 40.0%
CACAACTGAATGCAATCAAG+GGG + Chr2:43683256-43683275 None:intergenic 40.0%
CATAGCTTACAGGGTGAAAA+GGG + Chr2:43681979-43681998 None:intergenic 40.0%
CATCGTTGGTCAATTCATCT+AGG - Chr2:43682074-43682093 MsG0280009026.01.T01:intron 40.0%
CCATAGGTCAGTGAATCTAA+TGG + Chr2:43684429-43684448 None:intergenic 40.0%
CCATTAGATTCACTGACCTA+TGG - Chr2:43684426-43684445 MsG0280009026.01.T01:five_prime_UTR 40.0%
CTTCTATTTGCTTCATCCGT+TGG - Chr2:43683299-43683318 MsG0280009026.01.T01:intron 40.0%
GAGAGATTGGGAATAAGAGT+GGG - Chr2:43682300-43682319 MsG0280009026.01.T01:intron 40.0%
GCACAACTGAATGCAATCAA+GGG + Chr2:43683257-43683276 None:intergenic 40.0%
TAAGTGTTAACGATTGCGGA+AGG - Chr2:43682402-43682421 MsG0280009026.01.T01:intron 40.0%
TACATTGGGCAACTAACCAA+CGG + Chr2:43683318-43683337 None:intergenic 40.0%
TCAATCATTTGTACGGGCTT+TGG - Chr2:43683610-43683629 MsG0280009026.01.T01:intron 40.0%
TGGGCTTCAATCATTTGTAC+GGG - Chr2:43683604-43683623 MsG0280009026.01.T01:intron 40.0%
TGTATTCAGATCCAACTCTC+AGG + Chr2:43683429-43683448 None:intergenic 40.0%
TTACTTGCCTAAGGTGGAAA+AGG - Chr2:43682892-43682911 MsG0280009026.01.T01:intron 40.0%
TTAGTAGGTGAATCAGCAGT+AGG - Chr2:43684189-43684208 MsG0280009026.01.T01:intron 40.0%
TTGCTAACATTGTGCTGCAT+AGG + Chr2:43682935-43682954 None:intergenic 40.0%
TTTGACTAGAAGGCTGAGTA+TGG - Chr2:43684225-43684244 MsG0280009026.01.T01:intron 40.0%
! ACGAGTGCAATGTGAGTTTT+GGG - Chr2:43684134-43684153 MsG0280009026.01.T01:CDS 40.0%
! ATAGGGTATCTATGCTGGTT+AGG - Chr2:43682231-43682250 MsG0280009026.01.T01:intron 40.0%
! CATCCATACCTTTTGTGACT+AGG + Chr2:43684499-43684518 None:intergenic 40.0%
! CATTTTACGGCGACTCAAAA+TGG + Chr2:43682446-43682465 None:intergenic 40.0%
! CTAGTCACAAAAGGTATGGA+TGG - Chr2:43684497-43684516 MsG0280009026.01.T01:five_prime_UTR 40.0%
! CTCGTTTGTGTTAAGTTGTG+AGG - Chr2:43681623-43681642 MsG0280009026.01.T01:CDS 40.0%
! GAGGGTTTGTTTCATCATCA+TGG - Chr2:43683985-43684004 MsG0280009026.01.T01:intron 40.0%
! GGTAGTATATTTGGTGGTAG+TGG - Chr2:43681887-43681906 MsG0280009026.01.T01:intron 40.0%
! GTACTGACACTTCATGTTGA+AGG - Chr2:43682568-43682587 MsG0280009026.01.T01:intron 40.0%
! TCGTCCTTTTTGTGAGATAG+GGG - Chr2:43682325-43682344 MsG0280009026.01.T01:intron 40.0%
! TGGTTAGTTGCCCAATGTAA+TGG - Chr2:43683319-43683338 MsG0280009026.01.T01:intron 40.0%
!! AGTATATTTGGTGGTAGTGG+TGG - Chr2:43681890-43681909 MsG0280009026.01.T01:intron 40.0%
!! ATGTGCTTTTTGCAGCAAGA+AGG - Chr2:43683162-43683181 MsG0280009026.01.T01:CDS 40.0%
!! CTATTCAGTACCGACATATG+TGG - Chr2:43682603-43682622 MsG0280009026.01.T01:intron 40.0%
!!! GACTCAAAATGGCGGATTTT+TGG + Chr2:43682435-43682454 None:intergenic 40.0%
ACAACTGTCAGGGCCAATTT+GGG + Chr2:43682488-43682507 None:intergenic 45.0%
ACTGCATTTGGAGGAAGACA+AGG - Chr2:43683227-43683246 MsG0280009026.01.T01:CDS 45.0%
ATTTGTTTGGGTGCAGTGGA+GGG + Chr2:43684347-43684366 None:intergenic 45.0%
CAATCCAACTTGCCTTGTGA+TGG + Chr2:43683694-43683713 None:intergenic 45.0%
CAATGTTAATCTCGGAGCTG+AGG - Chr2:43683966-43683985 MsG0280009026.01.T01:intron 45.0%
CAGCTCCGAGATTAACATTG+AGG + Chr2:43683966-43683985 None:intergenic 45.0%
CATCTTGCCCTTAGAGAACA+TGG - Chr2:43683847-43683866 MsG0280009026.01.T01:intron 45.0%
CCAGGAATCCTTAGTTGTAC+CGG - Chr2:43682844-43682863 MsG0280009026.01.T01:CDS 45.0%
CTAACATTGTGCTGCATAGG+AGG + Chr2:43682932-43682951 None:intergenic 45.0%
CTCCGAGATTAACATTGAGG+AGG + Chr2:43683963-43683982 None:intergenic 45.0%
GAGATTAACATTGAGGAGGC+TGG + Chr2:43683959-43683978 None:intergenic 45.0%
GATCCATACAGTGAAGTGTG+AGG - Chr2:43683928-43683947 MsG0280009026.01.T01:intron 45.0%
GGAAATGTGGAGAGCTGATT+TGG - Chr2:43684051-43684070 MsG0280009026.01.T01:intron 45.0%
GGCAATGCCTCATACAGTTT+AGG - Chr2:43682111-43682130 MsG0280009026.01.T01:intron 45.0%
GGCACAACTGAATGCAATCA+AGG + Chr2:43683258-43683277 None:intergenic 45.0%
GGGCTCAAACAAAACAGAGT+TGG + Chr2:43684104-43684123 None:intergenic 45.0%
GTAACTCGTCGTTGTCACAT+TGG - Chr2:43682024-43682043 MsG0280009026.01.T01:intron 45.0%
TATGAAGTAACCCGGTAGGT+AGG - Chr2:43684030-43684049 MsG0280009026.01.T01:intron 45.0%
TATTTGTTTGGGTGCAGTGG+AGG + Chr2:43684348-43684367 None:intergenic 45.0%
TCGCCTAGTCACAAAAGGTA+TGG - Chr2:43684493-43684512 MsG0280009026.01.T01:five_prime_UTR 45.0%
TCTCCACATTTCCTACCTAC+CGG + Chr2:43684044-43684063 None:intergenic 45.0%
TGTCGCTATCCTTCCCAAAT+TGG - Chr2:43682472-43682491 MsG0280009026.01.T01:intron 45.0%
TTAGATTGGAGCTGTTCGAC+TGG - Chr2:43684380-43684399 MsG0280009026.01.T01:CDS 45.0%
TTATGATGCAGCAGCCTGTA+GGG + Chr2:43683551-43683570 None:intergenic 45.0%
TTTACGGCGACTCAAAATGG+CGG + Chr2:43682443-43682462 None:intergenic 45.0%
TTTATGATGCAGCAGCCTGT+AGG + Chr2:43683552-43683571 None:intergenic 45.0%
! ACAGAGTTGGCTCAATCTAG+AGG + Chr2:43684091-43684110 None:intergenic 45.0%
! GACGAGTGCAATGTGAGTTT+TGG - Chr2:43684133-43684152 MsG0280009026.01.T01:CDS 45.0%
! GCTTTTTGCAGCAAGAAGGT+TGG - Chr2:43683166-43683185 MsG0280009026.01.T01:CDS 45.0%
! TTTTGCAGCAAGAAGGTTGG+TGG - Chr2:43683169-43683188 MsG0280009026.01.T01:CDS 45.0%
!! ATATTTGGTGGTAGTGGTGG+TGG - Chr2:43681893-43681912 MsG0280009026.01.T01:intron 45.0%
!!! ACAATTAAATAATAATTTTT+TGG - Chr2:43682718-43682737 MsG0280009026.01.T01:intron 5.0%
AACCCGGTAGGTAGGAAATG+TGG - Chr2:43684038-43684057 MsG0280009026.01.T01:intron 50.0%
CACCGAAAAGGATGAGTGAG+GGG + Chr2:43682758-43682777 None:intergenic 50.0%
CCAAGTCTAATGGTCCCTAC+AGG - Chr2:43683534-43683553 MsG0280009026.01.T01:intron 50.0%
CCACCGAAAAGGATGAGTGA+GGG + Chr2:43682759-43682778 None:intergenic 50.0%
CCATGTCGCCTAGTCACAAA+AGG - Chr2:43684488-43684507 MsG0280009026.01.T01:five_prime_UTR 50.0%
CCGGTACAACTAAGGATTCC+TGG + Chr2:43682847-43682866 None:intergenic 50.0%
CCTGTAGGGACCATTAGACT+TGG + Chr2:43683537-43683556 None:intergenic 50.0%
CTCAATCTAGAGGTCCAGCT+TGG + Chr2:43684081-43684100 None:intergenic 50.0%
CTCCACATTTCCTACCTACC+GGG + Chr2:43684043-43684062 None:intergenic 50.0%
GACAACTGTCAGGGCCAATT+TGG + Chr2:43682489-43682508 None:intergenic 50.0%
GACACCATCACAAGGCAAGT+TGG - Chr2:43683687-43683706 MsG0280009026.01.T01:intron 50.0%
GGGTGAAAAGGGGAATCTTC+TGG + Chr2:43681968-43681987 None:intergenic 50.0%
TGCTAGTGCATCCTGAGAGT+TGG - Chr2:43683415-43683434 MsG0280009026.01.T01:intron 50.0%
TTCTCCGTCGCAATCCACAT+TGG - Chr2:43682515-43682534 MsG0280009026.01.T01:intron 50.0%
TTTGTTTGGGTGCAGTGGAG+GGG + Chr2:43684346-43684365 None:intergenic 50.0%
! AAGTACACTAGCCTCCTCAG+CGG - Chr2:43684163-43684182 MsG0280009026.01.T01:CDS 50.0%
! ATGGCCAATGTGGATTGCGA+CGG + Chr2:43682522-43682541 None:intergenic 50.0%
! CCTCACTCATCCTTTTCGGT+GGG - Chr2:43682757-43682776 MsG0280009026.01.T01:intron 50.0%
! CCTTTTGTGACTAGGCGACA+TGG + Chr2:43684491-43684510 None:intergenic 50.0%
! GACCCCTCACTCATCCTTTT+CGG - Chr2:43682753-43682772 MsG0280009026.01.T01:intron 50.0%
CCCACCGAAAAGGATGAGTG+AGG + Chr2:43682760-43682779 None:intergenic 55.0%
CTATGGCCTTCCAACCTTGG+AGG - Chr2:43684443-43684462 MsG0280009026.01.T01:five_prime_UTR 55.0%
CTCCAAGGTTGGAAGGCCAT+AGG + Chr2:43684445-43684464 None:intergenic 55.0%
CTGTCAGGGCCAATTTGGGA+AGG + Chr2:43682484-43682503 None:intergenic 55.0%
GACCTATGGCCTTCCAACCT+TGG - Chr2:43684440-43684459 MsG0280009026.01.T01:five_prime_UTR 55.0%
TCACCTACTAAGCTCCGCTG+AGG + Chr2:43684180-43684199 None:intergenic 55.0%
! CCCTCACTCATCCTTTTCGG+TGG - Chr2:43682756-43682775 MsG0280009026.01.T01:intron 55.0%
!! AGTGGTGGTGGTGGTAGTAG+TGG - Chr2:43681905-43681924 MsG0280009026.01.T01:intron 55.0%
!! GCTGATTTGGAGCTCCAAGC+TGG - Chr2:43684064-43684083 MsG0280009026.01.T01:intron 55.0%
!! TTTGGTGGTAGTGGTGGTGG+TGG - Chr2:43681896-43681915 MsG0280009026.01.T01:intron 55.0%
CATGCCATGCCATGCCTCCA+AGG + Chr2:43684460-43684479 None:intergenic 60.0%
CCATGCCATGCCTCCAAGGT+TGG + Chr2:43684456-43684475 None:intergenic 60.0%
CCTACTAAGCTCCGCTGAGG+AGG + Chr2:43684177-43684196 None:intergenic 60.0%
CCTCCTCAGCGGAGCTTAGT+AGG - Chr2:43684174-43684193 MsG0280009026.01.T01:CDS 60.0%
GCCATGCCTCCAAGGTTGGA+AGG + Chr2:43684452-43684471 None:intergenic 60.0%
GCCTTCCAACCTTGGAGGCA+TGG - Chr2:43684448-43684467 MsG0280009026.01.T01:five_prime_UTR 60.0%
! CCAACCTTGGAGGCATGGCA+TGG - Chr2:43684453-43684472 MsG0280009026.01.T01:five_prime_UTR 60.0%
GTTTGGGTGCAGTGGAGGGG+TGG + Chr2:43684343-43684362 None:intergenic 65.0%
GTGCAGTGGAGGGGTGGAGG+TGG + Chr2:43684337-43684356 None:intergenic 70.0%
TGGGTGCAGTGGAGGGGTGG+AGG + Chr2:43684340-43684359 None:intergenic 70.0%
Chromosome Type Strat End Strand Name
Chr2 gene 43681549 43684535 43681549 ID=MsG0280009026.01;Name=MsG0280009026.01
Chr2 mRNA 43681549 43684535 43681549 ID=MsG0280009026.01.T01;Parent=MsG0280009026.01;Name=MsG0280009026.01.T01;_AED=0.47;_eAED=0.58;_QI=118|0.5|0.6|1|0.75|0.8|5|0|199
Chr2 exon 43684339 43684535 43684339 ID=MsG0280009026.01.T01:exon:17188;Parent=MsG0280009026.01.T01
Chr2 exon 43684096 43684196 43684096 ID=MsG0280009026.01.T01:exon:17189;Parent=MsG0280009026.01.T01
Chr2 exon 43683004 43683248 43683004 ID=MsG0280009026.01.T01:exon:17190;Parent=MsG0280009026.01.T01
Chr2 exon 43682836 43682907 43682836 ID=MsG0280009026.01.T01:exon:17191;Parent=MsG0280009026.01.T01
Chr2 exon 43681549 43681651 43681549 ID=MsG0280009026.01.T01:exon:17192;Parent=MsG0280009026.01.T01
Chr2 five_prime_UTR 43684418 43684535 43684418 ID=MsG0280009026.01.T01:five_prime_utr;Parent=MsG0280009026.01.T01
Chr2 CDS 43684339 43684417 43684339 ID=MsG0280009026.01.T01:cds;Parent=MsG0280009026.01.T01
Chr2 CDS 43684096 43684196 43684096 ID=MsG0280009026.01.T01:cds;Parent=MsG0280009026.01.T01
Chr2 CDS 43683004 43683248 43683004 ID=MsG0280009026.01.T01:cds;Parent=MsG0280009026.01.T01
Chr2 CDS 43682836 43682907 43682836 ID=MsG0280009026.01.T01:cds;Parent=MsG0280009026.01.T01
Chr2 CDS 43681549 43681651 43681549 ID=MsG0280009026.01.T01:cds;Parent=MsG0280009026.01.T01
Gene Sequence

>MsG0280009026.01.T01

ATGAGGTTATTGTTTTTGTTATTCATCGCTTTGTCTTTCACCTATGTCTTTGCTGAACAAATCTTCCCTGTTCATATCGGTAGTATATTTGGTGGTAGTGGTGGTGGTGGTAGTAGTGGAAGCTCTCGTGAACCGAAATACAAGATTGAATTTCATCCAGAAGATTCCCCTTTTCACCCTGATAATGACCAGGAATCCTTAGTTGTACCGGATAAGAATGGACAGAAATTTATATGTTACTTGCCTAAGGTGGAAAAGGAAAAAAGTGGAAAGCCTCCTATGCAGCACAATGTTAGCAACATGATTGTTGAAACTGAGAAAAGGGTTAAACAGAAGACACCAGATGAACTATTAGAAATATTGAAGGGTCCATGCTTTATCAGAGTTAGTATTTGCATCTCTATATTTACTTTGAATGGCGACGTCAAGAAGGTTGGTGGTCTTATGAGTTTTGCTATCAAAAGAAATTTCGACAACTGCATTTGGAGGAAGACAAGATTCACTGACCTATGGCCTTCCAACCTTGGAGGCATGGCATGGCATGCTGTGAATCCATGTCGCCTAGTCACAAAAGGTATGGATGGAAATATGAAGAGTTGA
Protein sequence

>MsG0280009026.01.T01

MRLLFLLFIALSFTYVFAEQIFPVHIGSIFGGSGGGGSSGSSREPKYKIEFHPEDSPFHPDNDQESLVVPDKNGQKFICYLPKVEKEKSGKPPMQHNVSNMIVETEKRVKQKTPDELLEILKGPCFIRVSICISIFTLNGDVKKVGGLMSFAIKRNFDNCIWRKTRFTDLWPSNLGGMAWHAVNPCRLVTKGMDGNMKS*