Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MsG0280009221.01.T01 | KEH41327.1 | 84.716 | 229 | 26 | 2 | 1 | 229 | 1 | 220 | 3.79E-133 | 384 |
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MsG0280009221.01.T01 | Q9M1K1 | 38.298 | 235 | 127 | 5 | 1 | 228 | 1 | 224 | 2.41E-41 | 144 |
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MsG0280009221.01.T01 | A0A072VHQ7 | 84.716 | 229 | 26 | 2 | 1 | 229 | 1 | 220 | 1.81e-133 | 384 |
| Gene ID | Type | Classification |
|---|
| Gene ID | Type | Classification |
|---|
Co-expression Network
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|---|---|---|---|
| MsG0180002115.01 | MsG0280009221.01 | 0.819437 | 1.162170e-52 | 8.942886e-50 |
| MsG0180002579.01 | MsG0280009221.01 | 0.837361 | 5.440172e-57 | 7.049377e-54 |
| MsG0180005146.01 | MsG0280009221.01 | 0.801889 | 7.277474e-49 | 3.515513e-46 |
PPI
| Gene1 | Gene2 | Type |
|---|
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MsG0280009221.01.T01 | MTR_1g048720 | 84.716 | 229 | 26 | 2 | 1 | 229 | 1 | 220 | 4.59e-137 | 384 |
| MsG0280009221.01.T01 | MTR_1g048720 | 84.716 | 229 | 25 | 2 | 1 | 229 | 1 | 219 | 8.01e-137 | 383 |
| MsG0280009221.01.T01 | MTR_1g048720 | 84.649 | 228 | 26 | 2 | 1 | 228 | 1 | 219 | 1.19e-135 | 381 |
| MsG0280009221.01.T01 | MTR_1g048720 | 84.649 | 228 | 25 | 2 | 1 | 228 | 1 | 218 | 2.36e-135 | 380 |
| MsG0280009221.01.T01 | MTR_1g048710 | 81.140 | 228 | 25 | 3 | 1 | 228 | 1 | 210 | 6.60e-118 | 336 |
| MsG0280009221.01.T01 | MTR_1g048750 | 76.211 | 227 | 37 | 3 | 1 | 227 | 1 | 210 | 1.69e-117 | 336 |
| MsG0280009221.01.T01 | MTR_7g090410 | 51.261 | 238 | 88 | 8 | 1 | 228 | 1 | 220 | 6.00e-51 | 166 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MsG0280009221.01.T01 | AT3G56970 | 38.298 | 235 | 127 | 5 | 1 | 228 | 1 | 224 | 2.45e-42 | 144 |
| MsG0280009221.01.T01 | AT3G56980 | 37.354 | 257 | 104 | 6 | 1 | 228 | 1 | 229 | 4.46e-35 | 125 |
| MsG0280009221.01.T01 | AT2G41240 | 34.322 | 236 | 127 | 5 | 1 | 228 | 1 | 216 | 3.00e-32 | 117 |
| MsG0280009221.01.T01 | AT2G41240 | 34.322 | 236 | 126 | 6 | 1 | 228 | 1 | 215 | 3.15e-32 | 117 |
| MsG0280009221.01.T01 | AT5G04150 | 37.791 | 172 | 93 | 3 | 60 | 228 | 78 | 238 | 2.13e-25 | 100 |
| MsG0280009221.01.T01 | AT5G04150 | 37.209 | 172 | 94 | 3 | 60 | 228 | 78 | 238 | 1.53e-24 | 98.2 |
Find 35 sgRNAs with CRISPR-Local
Find 50 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| TCAACTTGTTTCTGTAATTC+TGG | 0.201065 | 2:+48092552 | None:intergenic |
| TCTTCATCTCAGACCTTTGC+AGG | 0.241758 | 2:-48092288 | MsG0280009221.01.T01:CDS |
| TGTAACTATGCTTCTTCCTT+TGG | 0.251062 | 2:+48092451 | None:intergenic |
| CTTCTATAATTTGCATTTCC+AGG | 0.265122 | 2:-48092259 | MsG0280009221.01.T01:CDS |
| CTCCCTTCGTTCACTTCTTC+CGG | 0.267513 | 2:-48092845 | MsG0280009221.01.T01:intron |
| GTAACTATGCTTCTTCCTTT+GGG | 0.307422 | 2:+48092452 | None:intergenic |
| AGGACTCTTGAAATTGTTGC+TGG | 0.367920 | 2:+48092585 | None:intergenic |
| AACAATTGGATGGCCCTTTG+AGG | 0.464251 | 2:-48093069 | MsG0280009221.01.T01:CDS |
| TGAAGGACTAAGTAAGAGAA+AGG | 0.469978 | 2:-48092532 | MsG0280009221.01.T01:CDS |
| CACATCAAGATCTCAGAAAG+TGG | 0.497267 | 2:+48092345 | None:intergenic |
| CATGCCAAGGTCACTGCTTG+TGG | 0.499479 | 2:+48092932 | None:intergenic |
| AGAATAACCTCTTCTTCTTG+AGG | 0.516991 | 2:+48092486 | None:intergenic |
| CCTAGTGAAATTAACACACC+TGG | 0.521834 | 2:+48092241 | None:intergenic |
| TGTAGAATGAATTCTGATGA+TGG | 0.529120 | 2:+48093030 | None:intergenic |
| ACAAATTATTGATCTTCTTG+CGG | 0.529574 | 2:+48092871 | None:intergenic |
| CTTCATCTCAGACCTTTGCA+GGG | 0.548412 | 2:-48092287 | MsG0280009221.01.T01:CDS |
| AAGAAGCATAGTTACAACTC+TGG | 0.551749 | 2:-48092444 | MsG0280009221.01.T01:CDS |
| ACTTTATCCTCAAGAAGAAG+AGG | 0.554683 | 2:-48092493 | MsG0280009221.01.T01:CDS |
| AGTGTAACATGAAATCTGAA+TGG | 0.555088 | 2:+48092380 | None:intergenic |
| GAATTACAGAAACAAGTTGA+AGG | 0.556198 | 2:-48092549 | MsG0280009221.01.T01:CDS |
| CATCCAATTGTTGAAAACAT+AGG | 0.558093 | 2:+48093080 | None:intergenic |
| AACCGGAAGAAGTGAACGAA+GGG | 0.560664 | 2:+48092843 | None:intergenic |
| TCTCAGAAAGTGGAATCTTG+TGG | 0.569417 | 2:+48092355 | None:intergenic |
| CAATCCACAAGCAGTGACCT+TGG | 0.569748 | 2:-48092936 | MsG0280009221.01.T01:CDS |
| TTGATGTGTTTAGAAAATGA+TGG | 0.577722 | 2:-48092330 | MsG0280009221.01.T01:CDS |
| ATAGAAGACTCTCCCTGCAA+AGG | 0.579465 | 2:+48092275 | None:intergenic |
| CCAATTGTTGAAAACATAGG+AGG | 0.582074 | 2:+48093083 | None:intergenic |
| ATGAATTCTGATGATGGGTA+TGG | 0.589276 | 2:+48093036 | None:intergenic |
| GTCACTGCTTGTGGATTGTG+AGG | 0.591686 | 2:+48092941 | None:intergenic |
| GTAGAATGAATTCTGATGAT+GGG | 0.591780 | 2:+48093031 | None:intergenic |
| CACAAGCAGTGACCTTGGCA+TGG | 0.602895 | 2:-48092931 | MsG0280009221.01.T01:CDS |
| TTCTAAGCAAAGAATCCCAA+AGG | 0.620328 | 2:-48092467 | MsG0280009221.01.T01:CDS |
| CAAGCTTCTTAACCATGCCA+AGG | 0.655760 | 2:+48092919 | None:intergenic |
| AAACCGGAAGAAGTGAACGA+AGG | 0.683817 | 2:+48092842 | None:intergenic |
| TCACTGCTTGTGGATTGTGA+GGG | 0.698792 | 2:+48092942 | None:intergenic |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|---|---|---|---|---|
| ! | ACAAATTATTGATCTTCTTG+CGG | + | Chr2:48092481-48092500 | None:intergenic | 25.0% |
| ! | ACAATTAGAAACAGAAAGAA+GGG | + | Chr2:48092557-48092576 | None:intergenic | 25.0% |
| ! | TCTAATTGTCAATAATGCTT+TGG | - | Chr2:48092568-48092587 | MsG0280009221.01.T01:CDS | 25.0% |
| ! | TTGATGTGTTTAGAAAATGA+TGG | - | Chr2:48093019-48093038 | MsG0280009221.01.T01:CDS | 25.0% |
| !! | GTAATTCTGGTATGTATTTT+AGG | + | Chr2:48092787-48092806 | None:intergenic | 25.0% |
| !!! | TAATTTTCATGATCAAGTTG+AGG | - | Chr2:48092364-48092383 | MsG0280009221.01.T01:CDS | 25.0% |
| AGTGTAACATGAAATCTGAA+TGG | + | Chr2:48092972-48092991 | None:intergenic | 30.0% | |
| CATCCAATTGTTGAAAACAT+AGG | + | Chr2:48092272-48092291 | None:intergenic | 30.0% | |
| GACAATTAGAAACAGAAAGA+AGG | + | Chr2:48092558-48092577 | None:intergenic | 30.0% | |
| GTAGAATGAATTCTGATGAT+GGG | + | Chr2:48092321-48092340 | None:intergenic | 30.0% | |
| TGTAGAATGAATTCTGATGA+TGG | + | Chr2:48092322-48092341 | None:intergenic | 30.0% | |
| ! | CTATGTTTTCAACAATTGGA+TGG | - | Chr2:48092270-48092289 | MsG0280009221.01.T01:CDS | 30.0% |
| ! | CTTCTATAATTTGCATTTCC+AGG | - | Chr2:48093090-48093109 | MsG0280009221.01.T01:CDS | 30.0% |
| ! | GAATTACAGAAACAAGTTGA+AGG | - | Chr2:48092800-48092819 | MsG0280009221.01.T01:intron | 30.0% |
| !! | GTTTTGTAGTTTCAAGTAGT+AGG | - | Chr2:48092927-48092946 | MsG0280009221.01.T01:CDS | 30.0% |
| !! | TCAACTTGTTTCTGTAATTC+TGG | + | Chr2:48092800-48092819 | None:intergenic | 30.0% |
| ACTTTATCCTCAAGAAGAAG+AGG | - | Chr2:48092856-48092875 | MsG0280009221.01.T01:CDS | 35.0% | |
| AGAATAACCTCTTCTTCTTG+AGG | + | Chr2:48092866-48092885 | None:intergenic | 35.0% | |
| AGTACCATTTGATCAGAAAC+CGG | + | Chr2:48092526-48092545 | None:intergenic | 35.0% | |
| CCAATTGTTGAAAACATAGG+AGG | + | Chr2:48092269-48092288 | None:intergenic | 35.0% | |
| GTAACTATGCTTCTTCCTTT+GGG | + | Chr2:48092900-48092919 | None:intergenic | 35.0% | |
| TCTTGAAAACTTGTGTGTTG+AGG | - | Chr2:48092624-48092643 | MsG0280009221.01.T01:CDS | 35.0% | |
| TGAAGGACTAAGTAAGAGAA+AGG | - | Chr2:48092817-48092836 | MsG0280009221.01.T01:intron | 35.0% | |
| TGTAACTATGCTTCTTCCTT+TGG | + | Chr2:48092901-48092920 | None:intergenic | 35.0% | |
| TTCTAAGCAAAGAATCCCAA+AGG | - | Chr2:48092882-48092901 | MsG0280009221.01.T01:CDS | 35.0% | |
| ! | AAGAAGCATAGTTACAACTC+TGG | - | Chr2:48092905-48092924 | MsG0280009221.01.T01:CDS | 35.0% |
| ! | CCTCCTATGTTTTCAACAAT+TGG | - | Chr2:48092266-48092285 | MsG0280009221.01.T01:CDS | 35.0% |
| !! | ATGAATTCTGATGATGGGTA+TGG | + | Chr2:48092316-48092335 | None:intergenic | 35.0% |
| !! | GTCTAATTGTGATGTCGTTT+TGG | - | Chr2:48092662-48092681 | MsG0280009221.01.T01:intron | 35.0% |
| AACTTGTGTGTTGAGGAGTA+TGG | - | Chr2:48092631-48092650 | MsG0280009221.01.T01:CDS | 40.0% | |
| AGGACTCTTGAAATTGTTGC+TGG | + | Chr2:48092767-48092786 | None:intergenic | 40.0% | |
| CACATCAAGATCTCAGAAAG+TGG | + | Chr2:48093007-48093026 | None:intergenic | 40.0% | |
| TCTCAGAAAGTGGAATCTTG+TGG | + | Chr2:48092997-48093016 | None:intergenic | 40.0% | |
| TCTTCCGGTTTCTGATCAAA+TGG | - | Chr2:48092519-48092538 | MsG0280009221.01.T01:CDS | 40.0% | |
| !!! | TGATGATGGGTATGGCTTTT+TGG | + | Chr2:48092308-48092327 | None:intergenic | 40.0% |
| AAACCGGAAGAAGTGAACGA+AGG | + | Chr2:48092510-48092529 | None:intergenic | 45.0% | |
| AACAATTGGATGGCCCTTTG+AGG | - | Chr2:48092280-48092299 | MsG0280009221.01.T01:CDS | 45.0% | |
| AACCGGAAGAAGTGAACGAA+GGG | + | Chr2:48092509-48092528 | None:intergenic | 45.0% | |
| ATAGAAGACTCTCCCTGCAA+AGG | + | Chr2:48093077-48093096 | None:intergenic | 45.0% | |
| CAAGCTTCTTAACCATGCCA+AGG | + | Chr2:48092433-48092452 | None:intergenic | 45.0% | |
| CTTCATCTCAGACCTTTGCA+GGG | - | Chr2:48093062-48093081 | MsG0280009221.01.T01:CDS | 45.0% | |
| TCTTCATCTCAGACCTTTGC+AGG | - | Chr2:48093061-48093080 | MsG0280009221.01.T01:CDS | 45.0% | |
| ! | TCACTGCTTGTGGATTGTGA+GGG | + | Chr2:48092410-48092429 | None:intergenic | 45.0% |
| !!! | ATGGCTTTTTGGCTCCTCAA+AGG | + | Chr2:48092297-48092316 | None:intergenic | 45.0% |
| !!! | TGGCTTTTTGGCTCCTCAAA+GGG | + | Chr2:48092296-48092315 | None:intergenic | 45.0% |
| CAATCCACAAGCAGTGACCT+TGG | - | Chr2:48092413-48092432 | MsG0280009221.01.T01:CDS | 50.0% | |
| CTCCCTTCGTTCACTTCTTC+CGG | - | Chr2:48092504-48092523 | MsG0280009221.01.T01:CDS | 50.0% | |
| ! | GTCACTGCTTGTGGATTGTG+AGG | + | Chr2:48092411-48092430 | None:intergenic | 50.0% |
| CACAAGCAGTGACCTTGGCA+TGG | - | Chr2:48092418-48092437 | MsG0280009221.01.T01:CDS | 55.0% | |
| CATGCCAAGGTCACTGCTTG+TGG | + | Chr2:48092420-48092439 | None:intergenic | 55.0% |
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| Chr2 | gene | 48092251 | 48093120 | 48092251 | ID=MsG0280009221.01;Name=MsG0280009221.01 |
| Chr2 | mRNA | 48092251 | 48093120 | 48092251 | ID=MsG0280009221.01.T01;Parent=MsG0280009221.01;Name=MsG0280009221.01.T01;_AED=0.51;_eAED=0.53;_QI=0|0|0|1|1|1|2|0|229 |
| Chr2 | exon | 48092846 | 48093120 | 48092846 | ID=MsG0280009221.01.T01:exon:44726;Parent=MsG0280009221.01.T01 |
| Chr2 | exon | 48092251 | 48092665 | 48092251 | ID=MsG0280009221.01.T01:exon:44725;Parent=MsG0280009221.01.T01 |
| Chr2 | CDS | 48092846 | 48093120 | 48092846 | ID=MsG0280009221.01.T01:cds;Parent=MsG0280009221.01.T01 |
| Chr2 | CDS | 48092251 | 48092665 | 48092251 | ID=MsG0280009221.01.T01:cds;Parent=MsG0280009221.01.T01 |
| Gene Sequence |
| Protein sequence |
