Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MsG0180002579.01.T04 | KEH41327.1 | 92.254 | 142 | 11 | 0 | 9 | 150 | 79 | 220 | 3.90E-86 | 261 |
| MsG0180002579.01.T02 | XP_013467291.2 | 91.124 | 169 | 15 | 0 | 9 | 177 | 79 | 247 | 5.72E-102 | 303 |
| MsG0180002579.01.T03 | KEH41327.1 | 92.254 | 142 | 11 | 0 | 38 | 179 | 79 | 220 | 1.13E-85 | 261 |
| MsG0180002579.01.T01 | KEH41328.1 | 91.124 | 169 | 15 | 0 | 38 | 206 | 79 | 247 | 1.54E-101 | 303 |
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MsG0180002579.01.T01 | Q9M1K1 | 45.181 | 166 | 83 | 3 | 38 | 199 | 86 | 247 | 2.48E-37 | 133 |
| MsG0180002579.01.T02 | Q9M1K1 | 45.181 | 166 | 83 | 3 | 9 | 170 | 86 | 247 | 8.92E-38 | 133 |
| MsG0180002579.01.T03 | Q9M1K1 | 48.227 | 141 | 71 | 1 | 38 | 178 | 86 | 224 | 1.55E-36 | 130 |
| MsG0180002579.01.T04 | Q9M1K1 | 48.227 | 141 | 71 | 1 | 9 | 149 | 86 | 224 | 5.13E-37 | 130 |
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MsG0180002579.01.T01 | A0A072VIS7 | 91.124 | 169 | 15 | 0 | 38 | 206 | 79 | 247 | 7.36e-102 | 303 |
| MsG0180002579.01.T02 | A0A396JNS6 | 91.124 | 169 | 15 | 0 | 9 | 177 | 79 | 247 | 2.73e-102 | 303 |
| MsG0180002579.01.T03 | A0A072VHQ7 | 92.254 | 142 | 11 | 0 | 38 | 179 | 79 | 220 | 5.39e-86 | 261 |
| MsG0180002579.01.T04 | A0A072VHQ7 | 92.254 | 142 | 11 | 0 | 9 | 150 | 79 | 220 | 1.86e-86 | 261 |
| Gene ID | Type | Classification |
|---|
| Gene ID | Type | Classification |
|---|
Co-expression Network
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|---|---|---|---|
| MsG0180002115.01 | MsG0180002579.01 | 0.825807 | 3.831862e-54 | 3.529803e-51 |
| MsG0180002579.01 | MsG0280009221.01 | 0.837361 | 5.440172e-57 | 7.049377e-54 |
| MsG0180002579.01 | MsG0880044712.01 | 0.803132 | 4.034247e-49 | 2.011335e-46 |
PPI
| Gene1 | Gene2 | Type |
|---|
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MsG0180002579.01.T04 | MTR_1g048720 | 92.254 | 142 | 11 | 0 | 9 | 150 | 79 | 220 | 4.73e-90 | 261 |
| MsG0180002579.01.T04 | MTR_1g048720 | 92.199 | 141 | 11 | 0 | 9 | 149 | 79 | 219 | 2.16e-88 | 258 |
| MsG0180002579.01.T04 | MTR_1g048720 | 91.549 | 142 | 11 | 1 | 9 | 150 | 79 | 219 | 2.32e-87 | 254 |
| MsG0180002579.01.T04 | MTR_1g048720 | 91.489 | 141 | 11 | 1 | 9 | 149 | 79 | 218 | 7.85e-86 | 251 |
| MsG0180002579.01.T04 | MTR_1g048750 | 84.286 | 140 | 22 | 0 | 9 | 148 | 71 | 210 | 3.21e-83 | 246 |
| MsG0180002579.01.T04 | MTR_1g048710 | 91.489 | 141 | 11 | 1 | 9 | 149 | 71 | 210 | 1.04e-79 | 236 |
| MsG0180002579.01.T04 | MTR_7g090410 | 69.718 | 142 | 41 | 2 | 9 | 149 | 80 | 220 | 6.27e-48 | 155 |
| MsG0180002579.01.T02 | MTR_1g048720 | 91.124 | 169 | 15 | 0 | 9 | 177 | 79 | 247 | 1.07e-105 | 303 |
| MsG0180002579.01.T02 | MTR_1g048720 | 90.533 | 169 | 15 | 1 | 9 | 177 | 79 | 246 | 3.12e-103 | 296 |
| MsG0180002579.01.T02 | MTR_1g048710 | 88.554 | 166 | 18 | 1 | 9 | 174 | 71 | 235 | 4.58e-93 | 271 |
| MsG0180002579.01.T02 | MTR_1g048720 | 92.199 | 141 | 11 | 0 | 9 | 149 | 79 | 219 | 3.08e-88 | 258 |
| MsG0180002579.01.T02 | MTR_1g048720 | 91.489 | 141 | 11 | 1 | 9 | 149 | 79 | 218 | 1.08e-85 | 251 |
| MsG0180002579.01.T02 | MTR_1g048750 | 84.286 | 140 | 22 | 0 | 9 | 148 | 71 | 210 | 5.15e-83 | 246 |
| MsG0180002579.01.T02 | MTR_7g090410 | 67.073 | 164 | 52 | 2 | 9 | 171 | 80 | 242 | 1.57e-53 | 171 |
| MsG0180002579.01.T03 | MTR_1g048720 | 92.254 | 142 | 11 | 0 | 38 | 179 | 79 | 220 | 1.37e-89 | 261 |
| MsG0180002579.01.T03 | MTR_1g048720 | 92.199 | 141 | 11 | 0 | 38 | 178 | 79 | 219 | 8.18e-88 | 258 |
| MsG0180002579.01.T03 | MTR_1g048720 | 91.549 | 142 | 11 | 1 | 38 | 179 | 79 | 219 | 5.74e-87 | 254 |
| MsG0180002579.01.T03 | MTR_1g048720 | 91.489 | 141 | 11 | 1 | 38 | 178 | 79 | 218 | 2.23e-85 | 251 |
| MsG0180002579.01.T03 | MTR_1g048750 | 84.286 | 140 | 22 | 0 | 38 | 177 | 71 | 210 | 2.13e-82 | 245 |
| MsG0180002579.01.T03 | MTR_1g048710 | 91.489 | 141 | 11 | 1 | 38 | 178 | 71 | 210 | 3.77e-79 | 235 |
| MsG0180002579.01.T03 | MTR_7g090410 | 69.718 | 142 | 41 | 2 | 38 | 178 | 80 | 220 | 2.07e-47 | 155 |
| MsG0180002579.01.T01 | MTR_1g048720 | 91.124 | 169 | 15 | 0 | 38 | 206 | 79 | 247 | 1.86e-105 | 303 |
| MsG0180002579.01.T01 | MTR_1g048720 | 90.533 | 169 | 15 | 1 | 38 | 206 | 79 | 246 | 6.87e-103 | 297 |
| MsG0180002579.01.T01 | MTR_1g048710 | 88.554 | 166 | 18 | 1 | 38 | 203 | 71 | 235 | 1.80e-92 | 270 |
| MsG0180002579.01.T01 | MTR_1g048720 | 92.199 | 141 | 11 | 0 | 38 | 178 | 79 | 219 | 7.92e-88 | 258 |
| MsG0180002579.01.T01 | MTR_1g048720 | 91.489 | 141 | 11 | 1 | 38 | 178 | 79 | 218 | 3.28e-85 | 251 |
| MsG0180002579.01.T01 | MTR_1g048750 | 84.286 | 140 | 22 | 0 | 38 | 177 | 71 | 210 | 3.36e-82 | 245 |
| MsG0180002579.01.T01 | MTR_7g090410 | 67.073 | 164 | 52 | 2 | 38 | 200 | 80 | 242 | 4.70e-53 | 171 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MsG0180002579.01.T04 | AT3G56970 | 48.227 | 141 | 71 | 1 | 9 | 149 | 86 | 224 | 5.23e-38 | 130 |
| MsG0180002579.01.T04 | AT3G56980 | 48.227 | 141 | 71 | 1 | 9 | 149 | 91 | 229 | 7.13e-31 | 112 |
| MsG0180002579.01.T04 | AT2G41240 | 41.844 | 141 | 82 | 0 | 9 | 149 | 76 | 216 | 2.32e-28 | 105 |
| MsG0180002579.01.T04 | AT5G04150 | 41.844 | 141 | 80 | 1 | 9 | 149 | 100 | 238 | 1.50e-27 | 102 |
| MsG0180002579.01.T04 | AT5G04150 | 41.135 | 141 | 81 | 1 | 9 | 149 | 100 | 238 | 9.16e-27 | 101 |
| MsG0180002579.01.T04 | AT2G41240 | 41.135 | 141 | 82 | 1 | 9 | 149 | 76 | 215 | 7.43e-26 | 98.6 |
| MsG0180002579.01.T02 | AT3G56970 | 45.181 | 166 | 83 | 3 | 9 | 170 | 86 | 247 | 9.09e-39 | 133 |
| MsG0180002579.01.T02 | AT3G56980 | 44.578 | 166 | 88 | 2 | 9 | 172 | 91 | 254 | 2.04e-32 | 117 |
| MsG0180002579.01.T02 | AT2G41240 | 40.244 | 164 | 97 | 1 | 9 | 172 | 76 | 238 | 5.74e-30 | 110 |
| MsG0180002579.01.T02 | AT2G41240 | 39.634 | 164 | 97 | 2 | 9 | 172 | 76 | 237 | 1.95e-27 | 103 |
| MsG0180002579.01.T02 | AT5G04150 | 41.844 | 141 | 80 | 1 | 9 | 149 | 100 | 238 | 5.72e-27 | 102 |
| MsG0180002579.01.T02 | AT5G04150 | 40.373 | 161 | 91 | 3 | 9 | 167 | 100 | 257 | 2.24e-26 | 101 |
| MsG0180002579.01.T03 | AT3G56970 | 48.227 | 141 | 71 | 1 | 38 | 178 | 86 | 224 | 1.58e-37 | 130 |
| MsG0180002579.01.T03 | AT3G56980 | 48.227 | 141 | 71 | 1 | 38 | 178 | 91 | 229 | 2.59e-30 | 111 |
| MsG0180002579.01.T03 | AT2G41240 | 41.844 | 141 | 82 | 0 | 38 | 178 | 76 | 216 | 6.06e-28 | 105 |
| MsG0180002579.01.T03 | AT5G04150 | 41.844 | 141 | 80 | 1 | 38 | 178 | 100 | 238 | 8.20e-27 | 102 |
| MsG0180002579.01.T03 | AT5G04150 | 41.135 | 141 | 81 | 1 | 38 | 178 | 100 | 238 | 5.74e-26 | 100 |
| MsG0180002579.01.T03 | AT2G41240 | 41.135 | 141 | 82 | 1 | 38 | 178 | 76 | 215 | 1.84e-25 | 98.6 |
| MsG0180002579.01.T01 | AT3G56970 | 45.181 | 166 | 83 | 3 | 38 | 199 | 86 | 247 | 2.53e-38 | 133 |
| MsG0180002579.01.T01 | AT3G56980 | 44.578 | 166 | 88 | 2 | 38 | 201 | 91 | 254 | 4.97e-32 | 117 |
| MsG0180002579.01.T01 | AT2G41240 | 40.244 | 164 | 97 | 1 | 38 | 201 | 76 | 238 | 1.11e-29 | 110 |
| MsG0180002579.01.T01 | AT2G41240 | 39.634 | 164 | 97 | 2 | 38 | 201 | 76 | 237 | 3.00e-27 | 103 |
| MsG0180002579.01.T01 | AT5G04150 | 41.844 | 141 | 80 | 1 | 38 | 178 | 100 | 238 | 2.74e-26 | 101 |
| MsG0180002579.01.T01 | AT5G04150 | 40.373 | 161 | 91 | 3 | 38 | 196 | 100 | 257 | 7.94e-26 | 100 |
Find 0 sgRNAs with CRISPR-Local
Find 52 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|---|---|---|---|---|
| !! | TTAAAAACATGTAAGAAATA+AGG | + | Chr1:40754856-40754875 | MsG0180002579.01.T01:intron | 15.0% |
| !! | TTACAAATAGTTGATTACTT+GGG | + | Chr1:40754918-40754937 | MsG0180002579.01.T01:intron | 20.0% |
| !! | TTTACAAATAGTTGATTACT+TGG | + | Chr1:40754917-40754936 | MsG0180002579.01.T01:intron | 20.0% |
| !!! | ATACAAATTATTGATTCTTG+CGG | - | Chr1:40753921-40753940 | None:intergenic | 20.0% |
| ! | AAGAAAGATTAAGAAGCAAA+AGG | - | Chr1:40754480-40754499 | None:intergenic | 25.0% |
| ! | ACAATTAGAAACAGAAAGAA+GGG | - | Chr1:40753995-40754014 | None:intergenic | 25.0% |
| ! | TCTAATTGTCAATAATGCTT+TGG | + | Chr1:40754006-40754025 | MsG0180002579.01.T01:intron | 25.0% |
| ! | TTGATATGTTTGGAAAATGA+CGG | + | Chr1:40754454-40754473 | MsG0180002579.01.T01:CDS | 25.0% |
| AGTGTAACATGATATCTGAA+TGG | - | Chr1:40754407-40754426 | None:intergenic | 30.0% | |
| GAAAACACATAAGTGCTTTA+AGG | - | Chr1:40755025-40755044 | None:intergenic | 30.0% | |
| GACAATTAGAAACAGAAAGA+AGG | - | Chr1:40753996-40754015 | None:intergenic | 30.0% | |
| GATGTATAGACTCATATAAG+AGG | + | Chr1:40754984-40755003 | MsG0180002579.01.T01:intron | 30.0% | |
| TGAGAAGCTCTTATCAATAA+TGG | + | Chr1:40755088-40755107 | MsG0180002579.01.T01:CDS | 30.0% | |
| ! | CTTCTATAATTTGCATTTCC+AGG | + | Chr1:40754525-40754544 | MsG0180002579.01.T01:CDS | 30.0% |
| ! | GAATTACAGAAACAAGTTGA+AGG | + | Chr1:40754235-40754254 | MsG0180002579.01.T01:CDS | 30.0% |
| ! | TTATCAATAATGGAGAAGGA+AGG | + | Chr1:40755098-40755117 | MsG0180002579.01.T01:CDS | 30.0% |
| !! | GTTTTGTAGTTTCAAGTAGT+AGG | + | Chr1:40754362-40754381 | MsG0180002579.01.T01:CDS | 30.0% |
| !! | TCAACTTGTTTCTGTAATTC+TGG | - | Chr1:40754235-40754254 | None:intergenic | 30.0% |
| !! | TTCTGAGATCTTGATATGTT+TGG | + | Chr1:40754444-40754463 | MsG0180002579.01.T01:CDS | 30.0% |
| !!! | TCTAATTGTGATGTCGTTTT+TGG | + | Chr1:40754100-40754119 | MsG0180002579.01.T01:intron | 30.0% |
| AGAATAACCTCTTCTTCTTG+AGG | - | Chr1:40754301-40754320 | None:intergenic | 35.0% | |
| AGTACCATTTGATCAGAAAC+AGG | - | Chr1:40753965-40753984 | None:intergenic | 35.0% | |
| CATATCAAGATCTCAGAAAG+TGG | - | Chr1:40754442-40754461 | None:intergenic | 35.0% | |
| GTAACTATGCTTCTTCCTTT+GGG | - | Chr1:40754335-40754354 | None:intergenic | 35.0% | |
| TGAACTCTTGAAATTGTTGC+TGG | - | Chr1:40754202-40754221 | None:intergenic | 35.0% | |
| TGAAGGACTAAGTAAGAGAA+AGG | + | Chr1:40754252-40754271 | MsG0180002579.01.T01:CDS | 35.0% | |
| TGTAACTATGCTTCTTCCTT+TGG | - | Chr1:40754336-40754355 | None:intergenic | 35.0% | |
| TTCTAAGCAAAGAATCCCAA+AGG | + | Chr1:40754317-40754336 | MsG0180002579.01.T01:CDS | 35.0% | |
| ! | AAGAAGCATAGTTACAACTC+TGG | + | Chr1:40754340-40754359 | MsG0180002579.01.T01:CDS | 35.0% |
| ! | CTTGAAAACCTTGTGTTTTG+AGG | + | Chr1:40754061-40754080 | MsG0180002579.01.T01:intron | 35.0% |
| ! | GCTCTTATCAATAATGGAGA+AGG | + | Chr1:40755094-40755113 | MsG0180002579.01.T01:CDS | 35.0% |
| !! | TCTTCCTGTTTCTGATCAAA+TGG | + | Chr1:40753958-40753977 | MsG0180002579.01.T01:CDS | 35.0% |
| ACCATACTCCTCAAAACACA+AGG | - | Chr1:40754072-40754091 | None:intergenic | 40.0% | |
| ACTTTCTCCTCAAGAAGAAG+AGG | + | Chr1:40754291-40754310 | MsG0180002579.01.T01:CDS | 40.0% | |
| CCAGGTGTGTTAATTTCACT+AGG | + | Chr1:40754543-40754562 | MsG0180002579.01.T01:intron | 40.0% | |
| CCTAGTGAAATTAACACACC+TGG | - | Chr1:40754546-40754565 | None:intergenic | 40.0% | |
| CTTTCTTCATCTCAGACCTT+TGG | + | Chr1:40754493-40754512 | MsG0180002579.01.T01:CDS | 40.0% | |
| TCTCAGAAAGTGGAATCTTG+TGG | - | Chr1:40754432-40754451 | None:intergenic | 40.0% | |
| ! | ACTTATGTGTTTTCTGCAGG+TGG | + | Chr1:40755031-40755050 | MsG0180002579.01.T01:intron | 40.0% |
| ! | AGCACTTATGTGTTTTCTGC+AGG | + | Chr1:40755028-40755047 | MsG0180002579.01.T01:intron | 40.0% |
| !!! | ACCTTGTGTTTTGAGGAGTA+TGG | + | Chr1:40754068-40754087 | MsG0180002579.01.T01:intron | 40.0% |
| AGGAAGAAGTGAACGAAGTG+AGG | - | Chr1:40753945-40753964 | None:intergenic | 45.0% | |
| ATAGAAGACTCTCCCTCCAA+AGG | - | Chr1:40754512-40754531 | None:intergenic | 45.0% | |
| CAAGCTTCTTGACCATGCTA+AGG | - | Chr1:40753873-40753892 | None:intergenic | 45.0% | |
| CTTCATCTCAGACCTTTGGA+GGG | + | Chr1:40754497-40754516 | MsG0180002579.01.T01:CDS | 45.0% | |
| GATCGCGTTCACTAGCATTA+TGG | - | Chr1:40753897-40753916 | None:intergenic | 45.0% | |
| TCTTCATCTCAGACCTTTGG+AGG | + | Chr1:40754496-40754515 | MsG0180002579.01.T01:CDS | 45.0% | |
| TTCTGCAGGTGGATAAAGCA+CGG | + | Chr1:40755042-40755061 | MsG0180002579.01.T01:intron | 45.0% | |
| ! | TCACTGCTTGTGGATTGTGA+GGG | - | Chr1:40753850-40753869 | None:intergenic | 45.0% |
| CACAAGCAGTGACCTTAGCA+TGG | + | Chr1:40753858-40753877 | MsG0180002579.01.T01:CDS | 50.0% | |
| CATGCTAAGGTCACTGCTTG+TGG | - | Chr1:40753860-40753879 | None:intergenic | 50.0% | |
| ! | GTCACTGCTTGTGGATTGTG+AGG | - | Chr1:40753851-40753870 | None:intergenic | 50.0% |
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| Chr1 | gene | 40753812 | 40755139 | 40753812 | ID=MsG0180002579.01;Name=MsG0180002579.01 |
| Chr1 | mRNA | 40753812 | 40755139 | 40753812 | ID=MsG0180002579.01.T01;Parent=MsG0180002579.01;Name=MsG0180002579.01.T01;_AED=0.31;_eAED=0.50;_QI=0|0|0|1|0|0|3|0|206 |
| Chr1 | CDS | 40753812 | 40753979 | 40753812 | ID=MsG0180002579.01.T01:cds;Parent=MsG0180002579.01.T01 |
| Chr1 | CDS | 40754184 | 40754546 | 40754184 | ID=MsG0180002579.01.T01:cds;Parent=MsG0180002579.01.T01 |
| Chr1 | CDS | 40755050 | 40755139 | 40755050 | ID=MsG0180002579.01.T01:cds;Parent=MsG0180002579.01.T01 |
| Chr1 | mRNA | 40753899 | 40755139 | 40753899 | ID=MsG0180002579.01.T02;Parent=MsG0180002579.01;Name=MsG0180002579.01.T02;_AED=0.34;_eAED=0.44;_QI=0|0|0|1|0|0|3|0|177 |
| Chr1 | CDS | 40753899 | 40753979 | 40753899 | ID=MsG0180002579.01.T02:cds;Parent=MsG0180002579.01.T02 |
| Chr1 | CDS | 40754184 | 40754546 | 40754184 | ID=MsG0180002579.01.T02:cds;Parent=MsG0180002579.01.T02 |
| Chr1 | CDS | 40755050 | 40755139 | 40755050 | ID=MsG0180002579.01.T02:cds;Parent=MsG0180002579.01.T02 |
| Chr1 | mRNA | 40753812 | 40754555 | 40753812 | ID=MsG0180002579.01.T03;Parent=MsG0180002579.01;Name=MsG0180002579.01.T03;_AED=0.33;_eAED=0.54;_QI=0|0|0|1|0|0|2|0|179 |
| Chr1 | CDS | 40753812 | 40753979 | 40753812 | ID=MsG0180002579.01.T03:cds;Parent=MsG0180002579.01.T03 |
| Chr1 | CDS | 40754184 | 40754555 | 40754184 | ID=MsG0180002579.01.T03:cds;Parent=MsG0180002579.01.T03 |
| Chr1 | mRNA | 40753899 | 40754555 | 40753899 | ID=MsG0180002579.01.T04;Parent=MsG0180002579.01;Name=MsG0180002579.01.T04;_AED=0.36;_eAED=0.48;_QI=0|0|0|1|0|0|2|0|150 |
| Chr1 | CDS | 40753899 | 40753979 | 40753899 | ID=MsG0180002579.01.T04:cds;Parent=MsG0180002579.01.T04 |
| Chr1 | CDS | 40754184 | 40754555 | 40754184 | ID=MsG0180002579.01.T04:cds;Parent=MsG0180002579.01.T04 |
| Gene Sequence |
| Protein sequence |
