AlfalfaGEDB | MsG0280009254.01

M. sativa cultivar ZhongmuNo.1 / MsG0280009254.01

KEGG: -
GO: GO:0003677
PFAM: -

Nr:

Query id	Subject id	identity %	alignment length	mis matches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MsG0280009254.01.T01	XP_003595647.1	95.628	183	6	1	1	183	1	181	1.95E-124	358
MsG0280009254.01.T02	XP_003595647.1	93.678	174	9	1	1	174	10	181	6.14E-105	308

Swissprot:

Query id	Subject id	identity %	alignment length	mis matches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MsG0280009254.01.T01	O23659	42.308	130	73	1	53	180	185	314	2.37E-26	105
MsG0280009254.01.T02	O23659	42.308	130	73	1	44	171	185	314	1.80E-26	105

Trembl:

Query id	Subject id	identity %	alignment length	mis matches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MsG0280009254.01.T01	G7IQL4	95.628	183	6	1	1	183	1	181	9.29e-125	358
MsG0280009254.01.T02	G7IQL4	93.678	174	9	1	1	174	10	181	2.93e-105	308

TFs/TRs:

Gene ID	Type	Classification

Protein Kinases:

Gene ID	Type	Classification

Network:

Co-expression Network

Gene1	Gene2	correlation coefficient	p_value	FDR
MsG0280009254.01	MsG0280011270.01	0.813626	2.327711e-51	1.527860e-48

PPI

Gene1	Gene2	Type

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MsG0280009254.01.T01	MTR_2g058520	95.628	183	6	1	1	183	1	181	2.36e-128	358
MsG0280009254.01.T01	MTR_1g040600	51.969	127	55	2	55	181	37	157	5.12e-37	126
MsG0280009254.01.T01	MTR_1g045700	52.033	123	55	2	56	178	103	221	1.30e-33	119
MsG0280009254.01.T01	MTR_1g046880	41.096	146	83	3	35	179	28	171	3.85e-30	108
MsG0280009254.01.T01	MTR_1g045740	47.059	119	61	2	64	181	145	262	2.29e-29	109
MsG0280009254.01.T01	MTR_1g045720	47.826	115	58	2	64	177	145	258	2.93e-28	106
MsG0280009254.01.T01	MTR_6g004120	41.739	115	59	2	64	178	23	129	3.98e-26	97.4
MsG0280009254.01.T01	MTR_1g046720	46.903	113	57	2	67	178	7	117	3.86e-25	94.4
MsG0280009254.01.T01	MTR_1g046550	44.737	114	58	2	67	179	154	263	2.75e-24	95.9
MsG0280009254.01.T02	MTR_2g058520	93.678	174	9	1	1	174	10	181	7.43e-109	308
MsG0280009254.01.T02	MTR_1g040600	51.969	127	55	2	46	172	37	157	2.95e-37	126
MsG0280009254.01.T02	MTR_1g045700	52.459	122	54	2	48	169	104	221	7.35e-34	119
MsG0280009254.01.T02	MTR_1g046880	41.096	146	83	3	26	170	28	171	3.15e-30	108
MsG0280009254.01.T02	MTR_1g045740	46.667	120	60	3	55	172	145	262	1.61e-29	109
MsG0280009254.01.T02	MTR_1g045720	47.826	115	58	2	55	168	145	258	2.37e-28	106
MsG0280009254.01.T02	MTR_6g004120	41.739	115	59	2	55	169	23	129	3.41e-26	97.4
MsG0280009254.01.T02	MTR_1g046720	46.903	113	57	2	58	169	7	117	3.36e-25	94.4
MsG0280009254.01.T02	MTR_1g046550	44.737	114	58	2	58	170	154	263	2.28e-24	95.9

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MsG0280009254.01.T01	AT2G33720	42.308	130	73	1	53	180	185	314	2.41e-27	105
MsG0280009254.01.T01	AT1G78640	41.026	117	68	1	67	182	155	271	2.35e-21	88.6
MsG0280009254.01.T01	AT1G78635	38.261	115	65	3	67	180	121	230	4.85e-13	65.5
MsG0280009254.01.T01	AT4G02870	35.484	124	62	5	67	183	191	303	1.28e-12	65.1
MsG0280009254.01.T02	AT2G33720	42.308	130	73	1	44	171	185	314	1.83e-27	105
MsG0280009254.01.T02	AT1G78640	41.026	117	68	1	58	173	155	271	1.63e-21	88.6
MsG0280009254.01.T02	AT1G78635	38.261	115	65	3	58	171	121	230	3.73e-13	65.9
MsG0280009254.01.T02	AT4G02870	35.484	124	62	5	58	174	191	303	8.23e-13	65.5

Find 41 sgRNAs with CRISPR-Local

Find 42 sgRNAs with CRISPR-GE

CRISPR-Local

CRISPR-Local
sgRNA_sequence	on_target_score	Position	Region
ACTCTTGTTGGCTGCAGATT+TGG	0.274049	2:+48764689	MsG0280009254.01.T01:CDS
ATCCCTTGTTGTGGTTGTTT+TGG	0.294910	2:-48764581	None:intergenic
AAATTGAAAGCGCGGTTAAA+AGG	0.301153	2:-48764939	None:intergenic
TCCAAGAGCTATGTTCTTAT+CGG	0.309648	2:+48764846	MsG0280009254.01.T01:CDS
TCAATTTCTGTGTTCTTAAA+CGG	0.314770	2:+48764955	MsG0280009254.01.T01:CDS
TCCATGTCCCAAACATTAAC+CGG	0.319466	2:-48764780	None:intergenic
AAGGTTCCCCGGTTAATGTT+TGG	0.345850	2:+48764772	MsG0280009254.01.T01:CDS
CTTAGGAAAGGTGATGAGAT+TGG	0.348331	2:+48764903	MsG0280009254.01.T01:CDS
AGGTTCCCCGGTTAATGTTT+GGG	0.362498	2:+48764773	MsG0280009254.01.T01:CDS
CAATTTCTGTGTTCTTAAAC+GGG	0.365327	2:+48764956	MsG0280009254.01.T01:CDS
TATTCACCTTCGAGTTGAAA+TGG	0.386008	2:+48764449	MsG0280009254.01.T01:CDS
ACGCTAACTGAGAGCGATCT+AGG	0.437395	2:+48764654	MsG0280009254.01.T01:CDS
AATCTTGAGCAGACTCTTGT+TGG	0.441053	2:+48764677	MsG0280009254.01.T01:CDS
CCCGGTTAATGTTTGGGACA+TGG	0.453970	2:+48764779	MsG0280009254.01.T01:CDS
TCGTGAGAAGAAGAGAGCTT+AGG	0.463621	2:+48764886	MsG0280009254.01.T01:CDS
GTGATGAGATTGGATTTCAA+TGG	0.466257	2:+48764913	MsG0280009254.01.T01:CDS
CTCTTCTTCTCACGAAATCC+TGG	0.468900	2:-48764878	None:intergenic
TGATGAGATTGGATTTCAAT+GGG	0.472943	2:+48764914	MsG0280009254.01.T01:CDS
CAACCAAAACAACCACAACA+AGG	0.477985	2:+48764578	MsG0280009254.01.T01:CDS
TGAAACTTTACGATGATCCT+TGG	0.494086	2:+48764619	MsG0280009254.01.T01:CDS
AGCGTCTTCTTGATCTTCCA+AGG	0.503514	2:-48764636	None:intergenic
ATTAACCGGGGAACCTTCCT+CGG	0.507041	2:-48764766	None:intergenic
ACCGATAAGAACATAGCTCT+TGG	0.507849	2:-48764847	None:intergenic
ATCGTAAAGTTTCAGTGTTG+TGG	0.521144	2:-48764610	None:intergenic
CCATGTCCCAAACATTAACC+GGG	0.521873	2:-48764779	None:intergenic
AAATTACTTGATTATTATGG+CGG	0.546376	2:-48764984	None:intergenic
TGAAGTCTTCAAATTTGTAG+AGG	0.548492	2:-48764508	None:intergenic
AACCAAAACAACCACAACAA+GGG	0.549886	2:+48764579	MsG0280009254.01.T01:CDS
CTTTAGAACGAGTTCATGCA+TGG	0.550200	2:-48764811	None:intergenic
GCTATGTTCTTATCGGTAAG+TGG	0.553460	2:+48764853	MsG0280009254.01.T01:CDS
TGGACACTGATCCCTTGTTG+TGG	0.569491	2:-48764590	None:intergenic
TCTTATCGGTAAGTGGAACC+AGG	0.584047	2:+48764860	MsG0280009254.01.T01:CDS
ACTGTTCCATTTCAACTCGA+AGG	0.593351	2:-48764455	None:intergenic
ATGAACTCGTTCTAAAGCGA+TGG	0.606209	2:+48764817	MsG0280009254.01.T01:CDS
AGAAACCGAGGAAGGTTCCC+CGG	0.610148	2:+48764761	MsG0280009254.01.T01:CDS
GAACACAGAAATTGAAAGCG+CGG	0.614343	2:-48764947	None:intergenic
AGAAGAAGAGAGCTTAGGAA+AGG	0.615591	2:+48764891	MsG0280009254.01.T01:CDS
GCATCATTGACATCCAACAT+AGG	0.620636	2:-48764729	None:intergenic
TGCAAGAGCTGCAGAAACCG+AGG	0.649817	2:+48764749	MsG0280009254.01.T01:CDS
AGAGCTGCAGAAACCGAGGA+AGG	0.650010	2:+48764753	MsG0280009254.01.T01:CDS
CATGTCCCAAACATTAACCG+GGG	0.704262	2:-48764778	None:intergenic

CRISPR-GE

badsite warning	sgRNA_sequence	Strand	Position	Region	GC_content
!	TCAATTTCTGTGTTCTTAAA+CGG	+	Chr2:48764955-48764974	MsG0280009254.01.T01:CDS	25.0%
!!	GAAACAAATTTTACCTATGT+TGG	+	Chr2:48764716-48764735	MsG0280009254.01.T01:CDS	25.0%
	CAATTTCTGTGTTCTTAAAC+GGG	+	Chr2:48764956-48764975	MsG0280009254.01.T01:CDS	30.0%
	TGAAGTCTTCAAATTTGTAG+AGG	-	Chr2:48764511-48764530	None:intergenic	30.0%
	TGATGAGATTGGATTTCAAT+GGG	+	Chr2:48764914-48764933	MsG0280009254.01.T01:CDS	30.0%
	AACCAAAACAACCACAACAA+GGG	+	Chr2:48764579-48764598	MsG0280009254.01.T01:CDS	35.0%
	ATCGTAAAGTTTCAGTGTTG+TGG	-	Chr2:48764613-48764632	None:intergenic	35.0%
	GTGATGAGATTGGATTTCAA+TGG	+	Chr2:48764913-48764932	MsG0280009254.01.T01:CDS	35.0%
	TCCAAGAGCTATGTTCTTAT+CGG	+	Chr2:48764846-48764865	MsG0280009254.01.T01:CDS	35.0%
	TGAAACTTTACGATGATCCT+TGG	+	Chr2:48764619-48764638	MsG0280009254.01.T01:CDS	35.0%
	TGGAACAGTTTCAGAAAAAG+AGG	+	Chr2:48764469-48764488	MsG0280009254.01.T01:CDS	35.0%
!	AAATTGAAAGCGCGGTTAAA+AGG	-	Chr2:48764942-48764961	None:intergenic	35.0%
!!	TATTCACCTTCGAGTTGAAA+TGG	+	Chr2:48764449-48764468	MsG0280009254.01.T01:CDS	35.0%
	AATCTTGAGCAGACTCTTGT+TGG	+	Chr2:48764677-48764696	MsG0280009254.01.T01:CDS	40.0%
	ACCGATAAGAACATAGCTCT+TGG	-	Chr2:48764850-48764869	None:intergenic	40.0%
	ACTGTTCCATTTCAACTCGA+AGG	-	Chr2:48764458-48764477	None:intergenic	40.0%
	AGAAGAAGAGAGCTTAGGAA+AGG	+	Chr2:48764891-48764910	MsG0280009254.01.T01:CDS	40.0%
	CAACCAAAACAACCACAACA+AGG	+	Chr2:48764578-48764597	MsG0280009254.01.T01:CDS	40.0%
	CTTAGGAAAGGTGATGAGAT+TGG	+	Chr2:48764903-48764922	MsG0280009254.01.T01:CDS	40.0%
	GAACACAGAAATTGAAAGCG+CGG	-	Chr2:48764950-48764969	None:intergenic	40.0%
	GCATCATTGACATCCAACAT+AGG	-	Chr2:48764732-48764751	None:intergenic	40.0%
	GCTATGTTCTTATCGGTAAG+TGG	+	Chr2:48764853-48764872	MsG0280009254.01.T01:CDS	40.0%
	TCCATGTCCCAAACATTAAC+CGG	-	Chr2:48764783-48764802	None:intergenic	40.0%
!!	ATCCCTTGTTGTGGTTGTTT+TGG	-	Chr2:48764584-48764603	None:intergenic	40.0%
!!	ATGAACTCGTTCTAAAGCGA+TGG	+	Chr2:48764817-48764836	MsG0280009254.01.T01:CDS	40.0%
!!	CTTTAGAACGAGTTCATGCA+TGG	-	Chr2:48764814-48764833	None:intergenic	40.0%
	AAGGTTCCCCGGTTAATGTT+TGG	+	Chr2:48764772-48764791	MsG0280009254.01.T01:CDS	45.0%
	AGCGTCTTCTTGATCTTCCA+AGG	-	Chr2:48764639-48764658	None:intergenic	45.0%
	AGGTTCCCCGGTTAATGTTT+GGG	+	Chr2:48764773-48764792	MsG0280009254.01.T01:CDS	45.0%
	CATGTCCCAAACATTAACCG+GGG	-	Chr2:48764781-48764800	None:intergenic	45.0%
	CCATGTCCCAAACATTAACC+GGG	-	Chr2:48764782-48764801	None:intergenic	45.0%
	CTCTTCTTCTCACGAAATCC+TGG	-	Chr2:48764881-48764900	None:intergenic	45.0%
	TCGTGAGAAGAAGAGAGCTT+AGG	+	Chr2:48764886-48764905	MsG0280009254.01.T01:CDS	45.0%
	TCTTATCGGTAAGTGGAACC+AGG	+	Chr2:48764860-48764879	MsG0280009254.01.T01:CDS	45.0%
!	ACTCTTGTTGGCTGCAGATT+TGG	+	Chr2:48764689-48764708	MsG0280009254.01.T01:CDS	45.0%
	ACGCTAACTGAGAGCGATCT+AGG	+	Chr2:48764654-48764673	MsG0280009254.01.T01:CDS	50.0%
	ATTAACCGGGGAACCTTCCT+CGG	-	Chr2:48764769-48764788	None:intergenic	50.0%
	TGGACACTGATCCCTTGTTG+TGG	-	Chr2:48764593-48764612	None:intergenic	50.0%
!	CCCGGTTAATGTTTGGGACA+TGG	+	Chr2:48764779-48764798	MsG0280009254.01.T01:CDS	50.0%
	AGAAACCGAGGAAGGTTCCC+CGG	+	Chr2:48764761-48764780	MsG0280009254.01.T01:CDS	55.0%
	AGAGCTGCAGAAACCGAGGA+AGG	+	Chr2:48764753-48764772	MsG0280009254.01.T01:CDS	55.0%
	TGCAAGAGCTGCAGAAACCG+AGG	+	Chr2:48764749-48764768	MsG0280009254.01.T01:CDS	55.0%

Chromosome	Type	Strat	End	Strand	Name
Chr2	gene	48764441	48764992	48764441	ID=MsG0280009254.01;Name=MsG0280009254.01
Chr2	mRNA	48764441	48764992	48764441	ID=MsG0280009254.01.T01;Parent=MsG0280009254.01;Name=MsG0280009254.01.T01;_AED=0.01;_eAED=0.01;_QI=0\|-1\|0\|1\|-1\|1\|1\|0\|183
Chr2	exon	48764441	48764992	48764441	ID=MsG0280009254.01.T01:exon:44846;Parent=MsG0280009254.01.T01
Chr2	CDS	48764441	48764992	48764441	ID=MsG0280009254.01.T01:cds;Parent=MsG0280009254.01.T01
Chr2	mRNA	48764468	48764992	48764468	ID=MsG0280009254.01.T02;Parent=MsG0280009254.01;Name=MsG0280009254.01.T02;_AED=0.03;_eAED=0.03;_QI=0\|-1\|0\|1\|-1\|0\|1\|0\|174
Chr2	exon	48764468	48764992	48764468	ID=MsG0280009254.01.T02:exon:44847;Parent=MsG0280009254.01.T02
Chr2	CDS	48764468	48764992	48764468	ID=MsG0280009254.01.T02:cds;Parent=MsG0280009254.01.T02

Gene Sequence

>MsG0280009254.01.T01

ATGAACAATATTCACCTTCGAGTTGAAATGGAACAGTTTCAGAAAAAGAGGAAATCTTGTGATGAAGCCTCTACAAATTTGAAGACTTCAAGAAGAAGATTATGCAGCAACAAAAATGAAGAGCAAAACAACAACAACAACCAAAACAACCACAACAAGGGATCAGTGTCCACAACACTGAAACTTTACGATGATCCTTGGAAGATCAAGAAGACGCTAACTGAGAGCGATCTAGGAATCTTGAGCAGACTCTTGTTGGCTGCAGATTTGGTGAAGAAACAAATTTTACCTATGTTGGATGTCAATGATGCAAGAGCTGCAGAAACCGAGGAAGGTTCCCCGGTTAATGTTTGGGACATGGAAACAAATTCCATGCATGAACTCGTTCTAAAGCGATGGTCTTCATCCAAGAGCTATGTTCTTATCGGTAAGTGGAACCAGGATTTCGTGAGAAGAAGAGAGCTTAGGAAAGGTGATGAGATTGGATTTCAATGGGATCCTTTTAACCGCGCTTTCAATTTCTGTGTTCTTAAACGGGCTATACCGCCATAA

>MsG0280009254.01.T02

ATGGAACAGTTTCAGAAAAAGAGGAAATCTTGTGATGAAGCCTCTACAAATTTGAAGACTTCAAGAAGAAGATTATGCAGCAACAAAAATGAAGAGCAAAACAACAACAACAACCAAAACAACCACAACAAGGGATCAGTGTCCACAACACTGAAACTTTACGATGATCCTTGGAAGATCAAGAAGACGCTAACTGAGAGCGATCTAGGAATCTTGAGCAGACTCTTGTTGGCTGCAGATTTGGTGAAGAAACAAATTTTACCTATGTTGGATGTCAATGATGCAAGAGCTGCAGAAACCGAGGAAGGTTCCCCGGTTAATGTTTGGGACATGGAAACAAATTCCATGCATGAACTCGTTCTAAAGCGATGGTCTTCATCCAAGAGCTATGTTCTTATCGGTAAGTGGAACCAGGATTTCGTGAGAAGAAGAGAGCTTAGGAAAGGTGATGAGATTGGATTTCAATGGGATCCTTTTAACCGCGCTTTCAATTTCTGTGTTCTTAAACGGGCTATACCGCCATAA

Protein sequence

>MsG0280009254.01.T01

MNNIHLRVEMEQFQKKRKSCDEASTNLKTSRRRLCSNKNEEQNNNNNQNNHNKGSVSTTLKLYDDPWKIKKTLTESDLGILSRLLLAADLVKKQILPMLDVNDARAAETEEGSPVNVWDMETNSMHELVLKRWSSSKSYVLIGKWNQDFVRRRELRKGDEIGFQWDPFNRAFNFCVLKRAIPP*

>MsG0280009254.01.T02

MEQFQKKRKSCDEASTNLKTSRRRLCSNKNEEQNNNNNQNNHNKGSVSTTLKLYDDPWKIKKTLTESDLGILSRLLLAADLVKKQILPMLDVNDARAAETEEGSPVNVWDMETNSMHELVLKRWSSSKSYVLIGKWNQDFVRRRELRKGDEIGFQWDPFNRAFNFCVLKRAIPP*