AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar ZhongmuNo.1 / MsG0280011270.01


Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MsG0280011270.01.T01 MTR_2g101140 98.980 196 2 0 1 196 1 196 3.21e-145 402
MsG0280011270.01.T01 MTR_8g013990 67.839 199 54 5 1 196 3 194 7.04e-92 267
MsG0280011270.01.T01 MTR_8g021190 42.969 128 69 3 61 186 29 154 2.41e-29 106
MsG0280011270.01.T01 MTR_1g103550 47.191 89 41 2 62 148 2 86 2.79e-22 87.8
MsG0280011270.01.T01 MTR_5g006430 38.053 113 65 2 62 173 2 110 1.54e-20 82.8
MsG0280011270.01.T01 MTR_3g105340 52.778 72 33 1 52 122 1 72 2.84e-20 82.8
MsG0280011270.01.T01 MTR_3g109860 34.959 123 77 3 32 151 4 126 1.55e-17 75.9
MsG0280011270.01.T01 MTR_4g125010 50.725 69 33 1 61 128 23 91 6.34e-17 74.7
MsG0280011270.01.T01 MTR_4g125030 42.708 96 47 3 61 151 21 113 3.77e-16 71.6
MsG0280011270.01.T01 MTR_1g014930 41.111 90 39 3 53 135 4 86 2.26e-15 70.5
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MsG0280011270.01.T01 AT5G25840 50.276 181 59 4 36 196 4 173 3.80e-53 168
MsG0280011270.01.T01 AT1G79770 53.205 156 59 3 35 190 26 167 1.28e-48 156
MsG0280011270.01.T01 AT1G72510 33.537 164 90 5 39 186 5 165 9.00e-23 90.1
MsG0280011270.01.T01 AT1G72510 33.537 164 90 5 39 186 5 165 9.00e-23 90.1
MsG0280011270.01.T01 AT4G14819 40.777 103 50 3 62 164 2 93 5.42e-22 86.3
MsG0280011270.01.T01 AT3G22540 40.449 89 50 1 62 150 2 87 1.97e-21 85.1
MsG0280011270.01.T01 AT2G25780 36.250 160 88 5 31 185 1 151 1.13e-20 84.3
MsG0280011270.01.T01 AT2G09970 31.707 164 92 5 40 186 6 166 1.26e-20 84.7
MsG0280011270.01.T01 AT1G54095 37.410 139 75 4 61 189 14 150 5.62e-20 82.4
MsG0280011270.01.T01 AT5G20670 33.846 130 80 3 63 186 23 152 3.66e-18 77.8

Find 34 sgRNAs with CRISPR-Local

Find 43 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
TGTGTTAGATTTAATAAATT+TGG 0.209428 2:-81771994 MsG0280011270.01.T01:CDS
AGCTTCTATATAAGATTAAT+TGG 0.236977 2:+81771783 None:intergenic
GCATCTTCAACTTCAGTTAT+CGG 0.334901 2:+81772192 None:intergenic
GTGATAAATACTTAGGGAAA+TGG 0.349825 2:-81772110 MsG0280011270.01.T01:CDS
AGTTGAGATGAATTAGGTTT+TGG 0.358947 2:+81772297 None:intergenic
GCTTCAGCTTGAAACAAAAC+AGG 0.390939 2:+81771961 None:intergenic
CGTGTACGTGATAAATACTT+AGG 0.397786 2:-81772117 MsG0280011270.01.T01:CDS
TTGTTGAGTTGAGATGAATT+AGG 0.428907 2:+81772291 None:intergenic
TTTCACCACCTTTGTCTCTA+GGG 0.429554 2:+81771881 None:intergenic
TGATAAATACTTAGGGAAAT+GGG 0.445526 2:-81772109 MsG0280011270.01.T01:CDS
TGAGGCAGTGAAAGAAGAGT+TGG 0.465910 2:-81772070 MsG0280011270.01.T01:CDS
TTTGGAGCATACAATGTTGT+TGG 0.466523 2:+81772323 None:intergenic
TACTTAGGGAAATGGGTTTG+TGG 0.466697 2:-81772102 MsG0280011270.01.T01:CDS
GAGTAAAATGGAAGGGAGAA+GGG 0.485393 2:-81771917 MsG0280011270.01.T01:CDS
TTGATATCTCTTGTAATAGC+AGG 0.500356 2:+81771820 None:intergenic
AGAGTAAAATGGAAGGGAGA+AGG 0.512738 2:-81771918 MsG0280011270.01.T01:CDS
GTGTACGTGATAAATACTTA+GGG 0.514569 2:-81772116 MsG0280011270.01.T01:CDS
TCAACTATTATGGCACCTCA+TGG 0.537712 2:-81772375 None:intergenic
GTGGAAGCTGTGTTAAATCA+AGG 0.538899 2:+81772214 None:intergenic
GGTGGTGAAAAGAAAGGTGG+TGG 0.545436 2:-81771868 MsG0280011270.01.T01:CDS
TTAGATTTAATAAATTTGGA+AGG 0.546403 2:-81771990 MsG0280011270.01.T01:CDS
CATTACCTAGCTCAATCTCT+AGG 0.548665 2:-81772347 MsG0280011270.01.T01:CDS
GACAAAGGTGGTGAAAAGAA+AGG 0.558067 2:-81771874 MsG0280011270.01.T01:CDS
TTGGTCCTAGAGATTGAGCT+AGG 0.570237 2:+81772342 None:intergenic
GGTTTGTGGTTTGTGTTCTG+AGG 0.597680 2:-81772088 MsG0280011270.01.T01:CDS
TGGTGGGGAAAATATTGAAG+AGG 0.600750 2:-81772040 MsG0280011270.01.T01:CDS
TCTTCAACTTCAGTTATCGG+TGG 0.626778 2:+81772195 None:intergenic
TCATTCAACCCTAGAGACAA+AGG 0.638410 2:-81771889 MsG0280011270.01.T01:CDS
CTTCAGCTTGAAACAAAACA+GGG 0.640341 2:+81771962 None:intergenic
GTGGTGAAAAGAAAGGTGGT+GGG 0.641104 2:-81771867 MsG0280011270.01.T01:CDS
AAAGGTGGTGAAAAGAAAGG+TGG 0.652842 2:-81771871 MsG0280011270.01.T01:CDS
GATGCAAAGTGTGAATGTTG+TGG 0.690241 2:-81772174 MsG0280011270.01.T01:CDS
TTCAACCCTAGAGACAAAGG+TGG 0.741656 2:-81771886 MsG0280011270.01.T01:CDS
CTAGGTAATGAATCTCCATG+AGG 0.751297 2:+81772360 None:intergenic

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!! TGTGTTAGATTTAATAAATT+TGG - Chr2:81772170-81772189 MsG0280011270.01.T01:CDS 15.0%
!! TTAGATTTAATAAATTTGGA+AGG - Chr2:81772174-81772193 MsG0280011270.01.T01:CDS 15.0%
! TGAAAAAGAGTAAAATGGAA+GGG - Chr2:81772240-81772259 MsG0280011270.01.T01:CDS 25.0%
! TGATAAATACTTAGGGAAAT+GGG - Chr2:81772055-81772074 MsG0280011270.01.T01:CDS 25.0%
AAAGAAGAGTTGGAGAAAAA+TGG - Chr2:81772104-81772123 MsG0280011270.01.T01:CDS 30.0%
CTGAAAAAGAGTAAAATGGA+AGG - Chr2:81772239-81772258 MsG0280011270.01.T01:CDS 30.0%
GATGCTGAAAAAGAGTAAAA+TGG - Chr2:81772235-81772254 MsG0280011270.01.T01:CDS 30.0%
GTGATAAATACTTAGGGAAA+TGG - Chr2:81772054-81772073 MsG0280011270.01.T01:CDS 30.0%
GTGTACGTGATAAATACTTA+GGG - Chr2:81772048-81772067 MsG0280011270.01.T01:CDS 30.0%
TTGATATCTCTTGTAATAGC+AGG + Chr2:81772347-81772366 None:intergenic 30.0%
! AGTTGAGATGAATTAGGTTT+TGG + Chr2:81771870-81771889 None:intergenic 30.0%
!! TTGTTGAGTTGAGATGAATT+AGG + Chr2:81771876-81771895 None:intergenic 30.0%
AAGAGTTGGAGAAAAATGGT+GGG - Chr2:81772108-81772127 MsG0280011270.01.T01:CDS 35.0%
CGTGTACGTGATAAATACTT+AGG - Chr2:81772047-81772066 MsG0280011270.01.T01:CDS 35.0%
CTTCAGCTTGAAACAAAACA+GGG + Chr2:81772205-81772224 None:intergenic 35.0%
GCATCTTCAACTTCAGTTAT+CGG + Chr2:81771975-81771994 None:intergenic 35.0%
! TTTGGAGCATACAATGTTGT+TGG + Chr2:81771844-81771863 None:intergenic 35.0%
!! TGAATTAGGTTTTGGTGGTT+TGG + Chr2:81771862-81771881 None:intergenic 35.0%
!! TGAGATGAATTAGGTTTTGG+TGG + Chr2:81771867-81771886 None:intergenic 35.0%
AAAGGTGGTGAAAAGAAAGG+TGG - Chr2:81772293-81772312 MsG0280011270.01.T01:CDS 40.0%
AGAGTAAAATGGAAGGGAGA+AGG - Chr2:81772246-81772265 MsG0280011270.01.T01:CDS 40.0%
AGAGTTGGAGAAAAATGGTG+GGG - Chr2:81772109-81772128 MsG0280011270.01.T01:CDS 40.0%
CATTACCTAGCTCAATCTCT+AGG - Chr2:81771817-81771836 MsG0280011270.01.T01:CDS 40.0%
CTAGGTAATGAATCTCCATG+AGG + Chr2:81771807-81771826 None:intergenic 40.0%
GAAGAGTTGGAGAAAAATGG+TGG - Chr2:81772107-81772126 MsG0280011270.01.T01:CDS 40.0%
GACAAAGGTGGTGAAAAGAA+AGG - Chr2:81772290-81772309 MsG0280011270.01.T01:CDS 40.0%
GAGTAAAATGGAAGGGAGAA+GGG - Chr2:81772247-81772266 MsG0280011270.01.T01:CDS 40.0%
GATGCAAAGTGTGAATGTTG+TGG - Chr2:81771990-81772009 MsG0280011270.01.T01:CDS 40.0%
GCTTCAGCTTGAAACAAAAC+AGG + Chr2:81772206-81772225 None:intergenic 40.0%
GTGGAAGCTGTGTTAAATCA+AGG + Chr2:81771953-81771972 None:intergenic 40.0%
TCATTCAACCCTAGAGACAA+AGG - Chr2:81772275-81772294 MsG0280011270.01.T01:CDS 40.0%
TCTTCAACTTCAGTTATCGG+TGG + Chr2:81771972-81771991 None:intergenic 40.0%
TGGTGGGGAAAATATTGAAG+AGG - Chr2:81772124-81772143 MsG0280011270.01.T01:CDS 40.0%
TTTCACCACCTTTGTCTCTA+GGG + Chr2:81772286-81772305 None:intergenic 40.0%
! CGTACACGTTTTATGTACTC+AGG + Chr2:81772035-81772054 None:intergenic 40.0%
! TACTTAGGGAAATGGGTTTG+TGG - Chr2:81772062-81772081 MsG0280011270.01.T01:CDS 40.0%
! TTTTCACCACCTTTGTCTCT+AGG + Chr2:81772287-81772306 None:intergenic 40.0%
GTGGTGAAAAGAAAGGTGGT+GGG - Chr2:81772297-81772316 MsG0280011270.01.T01:CDS 45.0%
TGAGGCAGTGAAAGAAGAGT+TGG - Chr2:81772094-81772113 MsG0280011270.01.T01:CDS 45.0%
TTCAACCCTAGAGACAAAGG+TGG - Chr2:81772278-81772297 MsG0280011270.01.T01:CDS 45.0%
TTGGTCCTAGAGATTGAGCT+AGG + Chr2:81771825-81771844 None:intergenic 45.0%
! GGTTTGTGGTTTGTGTTCTG+AGG - Chr2:81772076-81772095 MsG0280011270.01.T01:CDS 45.0%
GGTGGTGAAAAGAAAGGTGG+TGG - Chr2:81772296-81772315 MsG0280011270.01.T01:CDS 50.0%
Chromosome Type Strat End Strand Name
Chr2 gene 81771798 81772388 81771798 ID=MsG0280011270.01;Name=MsG0280011270.01
Chr2 mRNA 81771798 81772388 81771798 ID=MsG0280011270.01.T01;Parent=MsG0280011270.01;Name=MsG0280011270.01.T01;_AED=0.48;_eAED=0.48;_QI=0|-1|0|1|-1|1|1|0|196
Chr2 exon 81771798 81772388 81771798 ID=MsG0280011270.01.T01:exon:2791;Parent=MsG0280011270.01.T01
Chr2 CDS 81771798 81772388 81771798 ID=MsG0280011270.01.T01:cds;Parent=MsG0280011270.01.T01
Gene Sequence

>MsG0280011270.01.T01

ATGGCACCTCATGGAGATTCATTACCTAGCTCAATCTCTAGGACCAACAACATTGTATGCTCCAAACCACCAAAACCTAATTCATCTCAACTCAACAACAATAATATTCAAAGAACAATCTCAGACATATCATTTGAACTATCAAAACAAGACCTTGATTTAACACAGCTTCCACCGATAACTGAAGTTGAAGATGCAAAGTGTGAATGTTGTGGCATGTCTGAAGAGTTTACACCTGAGTACATAAAACGTGTACGTGATAAATACTTAGGGAAATGGGTTTGTGGTTTGTGTTCTGAGGCAGTGAAAGAAGAGTTGGAGAAAAATGGTGGGGAAAATATTGAAGAGGCTTTGAGTGCACACATGAGTGCATGTGTTAGATTTAATAAATTTGGAAGGAGTTTCCCTGTTTTGTTTCAAGCTGAAGCTATGAAAGAGATGCTGAAAAAGAGTAAAATGGAAGGGAGAAGGGCTAAGTCATTCAACCCTAGAGACAAAGGTGGTGAAAAGAAAGGTGGTGGGCTTGCTAGAAGTTCAAGTTGCATTCCTGCTATTACAAGAGATATCAATGATTTCACAATCACCAATTAA

Protein sequence

>MsG0280011270.01.T01

MAPHGDSLPSSISRTNNIVCSKPPKPNSSQLNNNNIQRTISDISFELSKQDLDLTQLPPITEVEDAKCECCGMSEEFTPEYIKRVRDKYLGKWVCGLCSEAVKEELEKNGGENIEEALSAHMSACVRFNKFGRSFPVLFQAEAMKEMLKKSKMEGRRAKSFNPRDKGGEKKGGGLARSSSCIPAITRDINDFTITN*