Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MsG0280009649.01.T01 | RHN66618.1 | 87.234 | 94 | 12 | 0 | 1 | 94 | 408 | 501 | 9.15E-48 | 169 |
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MsG0280009649.01.T01 | A0A396IPF5 | 87.234 | 94 | 12 | 0 | 1 | 94 | 408 | 501 | 4.37e-48 | 169 |
| Gene ID | Type | Classification |
|---|
| Gene ID | Type | Classification |
|---|
Co-expression Network
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|---|---|---|---|
| MsG0180005268.01 | MsG0280009649.01 | 0.806844 | 6.752725e-50 | 3.703697e-47 |
| MsG0280009649.01 | MsG0780039943.01 | 0.806160 | 9.413782e-50 | 5.073636e-47 |
| MsG0280009649.01 | MsG0780040258.01 | 0.987752 | 2.090527e-171 | 1.146127e-164 |
| MsG0280009649.01 | MsG0780040259.01 | 0.867977 | 9.188021e-66 | 3.268792e-62 |
| MsG0280009649.01 | MsG0780041210.01 | 0.806225 | 9.119514e-50 | 4.923317e-47 |
| MsG0280009649.01 | MsG0880045869.01 | 0.818044 | 2.406542e-52 | 1.781851e-49 |
PPI
| Gene1 | Gene2 | Type |
|---|
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MsG0280009649.01.T01 | MTR_0168s0060 | 86.957 | 92 | 12 | 0 | 1 | 92 | 408 | 499 | 4.94e-50 | 165 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|
Find 10 sgRNAs with CRISPR-Local
Find 11 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| TAGAGCTTTAGAAGCTAAAT+TGG | 0.147902 | 2:-55807845 | MsG0280009649.01.T01:CDS |
| GTAATAATAGTCCTTCATTC+AGG | 0.418166 | 2:-55807777 | MsG0280009649.01.T01:CDS |
| ACTGTCAAAGTCAGAGTGTA+AGG | 0.462684 | 2:+55807639 | None:intergenic |
| GCAGATAAAACATATTTGCT+TGG | 0.490350 | 2:-55807715 | MsG0280009649.01.T01:CDS |
| GCTTCATTGAGCCTGAATGA+AGG | 0.509878 | 2:+55807766 | None:intergenic |
| GGATGTTTAATAGAAGAAAG+TGG | 0.585798 | 2:-55807802 | MsG0280009649.01.T01:CDS |
| TGACATGCTTCTCAAAAGAG+AGG | 0.614433 | 2:-55807875 | None:intergenic |
| TTCAGGCTCAATGAAGCAAG+AGG | 0.636416 | 2:-55807760 | MsG0280009649.01.T01:CDS |
| GCTCAATGAAGCAAGAGGAG+AGG | 0.657394 | 2:-55807755 | MsG0280009649.01.T01:CDS |
| TGTTTAATAGAAGAAAGTGG+TGG | 0.722197 | 2:-55807799 | MsG0280009649.01.T01:CDS |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|---|---|---|---|---|
| !!! | GAGTTTTATAGAAACAAATA+TGG | - | Chr2:55807654-55807673 | MsG0280009649.01.T01:CDS | 20.0% |
| GCAGATAAAACATATTTGCT+TGG | - | Chr2:55807762-55807781 | MsG0280009649.01.T01:CDS | 30.0% | |
| GGATGTTTAATAGAAGAAAG+TGG | - | Chr2:55807675-55807694 | MsG0280009649.01.T01:CDS | 30.0% | |
| GTAATAATAGTCCTTCATTC+AGG | - | Chr2:55807700-55807719 | MsG0280009649.01.T01:CDS | 30.0% | |
| TGTTTAATAGAAGAAAGTGG+TGG | - | Chr2:55807678-55807697 | MsG0280009649.01.T01:CDS | 30.0% | |
| ! | AGATCACACTTTTCAGAAAA+TGG | - | Chr2:55807807-55807826 | MsG0280009649.01.T01:CDS | 30.0% |
| !! | TAGAGCTTTAGAAGCTAAAT+TGG | - | Chr2:55807632-55807651 | MsG0280009649.01.T01:CDS | 30.0% |
| ACTGTCAAAGTCAGAGTGTA+AGG | + | Chr2:55807841-55807860 | None:intergenic | 40.0% | |
| GCTTCATTGAGCCTGAATGA+AGG | + | Chr2:55807714-55807733 | None:intergenic | 45.0% | |
| TTCAGGCTCAATGAAGCAAG+AGG | - | Chr2:55807717-55807736 | MsG0280009649.01.T01:CDS | 45.0% | |
| GCTCAATGAAGCAAGAGGAG+AGG | - | Chr2:55807722-55807741 | MsG0280009649.01.T01:CDS | 50.0% |
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| Chr2 | gene | 55807606 | 55807893 | 55807606 | ID=MsG0280009649.01;Name=MsG0280009649.01 |
| Chr2 | mRNA | 55807606 | 55807893 | 55807606 | ID=MsG0280009649.01.T01;Parent=MsG0280009649.01;Name=MsG0280009649.01.T01;_AED=0.47;_eAED=0.47;_QI=0|-1|0|1|-1|1|1|0|95 |
| Chr2 | exon | 55807606 | 55807893 | 55807606 | ID=MsG0280009649.01.T01:exon:16623;Parent=MsG0280009649.01.T01 |
| Chr2 | CDS | 55807606 | 55807893 | 55807606 | ID=MsG0280009649.01.T01:cds;Parent=MsG0280009649.01.T01 |
| Gene Sequence |
| Protein sequence |
