AlfalfaGEDB | MsG0280009967.01

M. sativa cultivar ZhongmuNo.1 / MsG0280009967.01

KEGG: -
GO: -
PFAM: -

Nr:

Query id	Subject id	identity %	alignment length	mis matches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MsG0280009967.01.T01	XP_003596253.2	94.505	91	5	0	1	91	1	91	1.50E-53	186

Swissprot:

Query id	Subject id	identity %	alignment length	mis matches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MsG0280009967.01.T01	C0LGE0	37.5	88	52	3	7	91	8	95	1.22E-12	65.1

Trembl:

Query id	Subject id	identity %	alignment length	mis matches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MsG0280009967.01.T01	G7IQY3	94.505	91	5	0	1	91	1	91	7.18e-54	186

TFs/TRs:

Gene ID	Type	Classification

Protein Kinases:

Gene ID	Type	Classification

Network:

Co-expression Network

Gene1	Gene2	correlation coefficient	p_value	FDR
MsG0280009967.01	MsG0280009968.01	0.829469	5.059025e-55	5.182401e-52

PPI

Gene1	Gene2	Type
MsG0280009967.01	MsG0880044266.01	PPI
MsG0280009967.01	MsG0080049102.01	PPI
MsG0880044213.01	MsG0280009967.01	PPI
MsG0080049102.01	MsG0280009967.01	PPI
MsG0280009967.01	MsG0080049102.01	PPI
MsG0280009967.01	MsG0080049102.01	PPI
MsG0280009967.01	MsG0880042321.01	PPI
MsG0280009967.01	MsG0880044213.01	PPI

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MsG0280009967.01.T01	MTR_2g075140	94.505	91	5	0	1	91	1	91	1.82e-57	186
MsG0280009967.01.T01	MTR_2g075140	94.505	91	5	0	1	91	1	91	2.87e-57	186
MsG0280009967.01.T01	MTR_2g074980	75.824	91	22	0	1	91	1	91	4.33e-47	160
MsG0280009967.01.T01	MTR_2g074840	73.196	97	20	1	1	91	1	97	2.62e-45	155
MsG0280009967.01.T01	MTR_2g074840	73.196	97	20	1	1	91	1	97	9.20e-45	153
MsG0280009967.01.T01	MTR_2g073650	76.042	96	18	2	1	91	1	96	1.17e-44	153
MsG0280009967.01.T01	MTR_2g074990	76.923	91	16	1	1	91	1	86	5.50e-44	151
MsG0280009967.01.T01	MTR_2g073650	76.042	96	18	2	1	91	1	96	2.11e-43	149
MsG0280009967.01.T01	MTR_2g075010	75.824	91	17	1	1	91	1	86	7.97e-43	148
MsG0280009967.01.T01	MTR_2g075060	74.737	95	20	1	1	91	1	95	1.01e-42	147
MsG0280009967.01.T01	MTR_2g074830	72.527	91	19	2	1	91	1	85	3.13e-39	135
MsG0280009967.01.T01	MTR_2g073600	41.304	92	48	2	1	91	5	91	4.39e-19	80.5
MsG0280009967.01.T01	MTR_8g058070	46.269	67	36	0	25	91	25	91	7.31e-19	75.1
MsG0280009967.01.T01	MTR_2g073560	41.304	92	48	2	1	91	5	91	9.77e-19	79.3
MsG0280009967.01.T01	MTR_8g059605	46.269	67	36	0	25	91	25	91	1.61e-17	75.9
MsG0280009967.01.T01	MTR_8g059615	46.269	67	36	0	25	91	25	91	1.87e-17	75.9
MsG0280009967.01.T01	MTR_8g059615	46.269	67	36	0	25	91	25	91	3.89e-17	75.1
MsG0280009967.01.T01	MTR_2g073520	38.043	92	51	2	1	91	5	91	1.48e-16	73.2
MsG0280009967.01.T01	MTR_2g073520	38.043	92	51	2	1	91	5	91	2.66e-16	72.4
MsG0280009967.01.T01	MTR_2g073640	41.667	84	47	2	8	91	14	95	5.87e-16	71.6
MsG0280009967.01.T01	MTR_5g083910	43.284	67	38	0	25	91	25	91	5.56e-15	68.9
MsG0280009967.01.T01	MTR_5g091950	40.426	94	53	2	1	91	1	94	8.55e-15	68.2
MsG0280009967.01.T01	MTR_2g073630	49.057	53	27	0	39	91	4	56	7.87e-14	65.5

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MsG0280009967.01.T01	AT1G07650	37.500	88	52	3	7	91	8	95	1.13e-13	65.1
MsG0280009967.01.T01	AT1G07650	37.500	88	52	3	7	91	8	95	1.24e-13	65.1
MsG0280009967.01.T01	AT1G07650	37.500	88	52	3	7	91	8	95	1.24e-13	65.1

Find 19 sgRNAs with CRISPR-Local

Find 27 sgRNAs with CRISPR-GE

CRISPR-Local

CRISPR-Local
sgRNA_sequence	on_target_score	Position	Region
ATTTGTAACTGCTCTGTTGC+TGG	0.276799	2:+62016901	MsG0280009967.01.T01:CDS
TCCTTTCACTCATTTCCATT+TGG	0.305708	2:+62016492	MsG0280009967.01.T01:CDS
CATGCAGCAACCAACCTAAC+TGG	0.341684	2:+62016839	MsG0280009967.01.T01:CDS
AGCTAAATCACTTGGTAAGA+AGG	0.385261	2:+62016795	MsG0280009967.01.T01:CDS
TTTGTAACTGCTCTGTTGCT+GGG	0.410716	2:+62016902	MsG0280009967.01.T01:CDS
ATATATATCATACTTTCATC+AGG	0.432179	2:-62016554	None:intergenic
ACATTGTTCTCTACGACATT+CGG	0.438923	2:-62016877	None:intergenic
AATCACTTGGTAAGAAGGAT+TGG	0.444651	2:+62016800	MsG0280009967.01.T01:CDS
TGCAAGAGATATGAACCAAA+TGG	0.499336	2:-62016507	None:intergenic
ATCGTAGGTGTAACCCAGTT+AGG	0.522934	2:-62016853	None:intergenic
CAGTTAGGTTGGTTGCTGCA+TGG	0.542042	2:-62016838	None:intergenic
ACCAAATGGAAATGAGTGAA+AGG	0.570336	2:-62016493	None:intergenic
ATGCAGCAACCAACCTAACT+GGG	0.575843	2:+62016840	MsG0280009967.01.T01:CDS
TAGGTGTAACCCAGTTAGGT+TGG	0.580103	2:-62016849	None:intergenic
TTGTAACTGCTCTGTTGCTG+GGG	0.590973	2:+62016903	MsG0280009967.01.T01:CDS
GAAGTTATAGCTAAATCACT+TGG	0.595314	2:+62016787	MsG0280009967.01.T01:CDS
ATACTTACATTTCAATAACA+TGG	0.610442	2:-62016936	None:intergenic
TCTACGACATTCGGTATCGT+AGG	0.634566	2:-62016868	None:intergenic
TATATATCATACTTTCATCA+GGG	0.639143	2:-62016553	None:intergenic

CRISPR-GE

badsite warning	sgRNA_sequence	Strand	Position	Region	GC_content
!!	AATATAAAGTAAAAAATGGG+AGG	-	Chr2:62016590-62016609	None:intergenic	20.0%
!!	ATAAAGTCAAACTCTTATTA+TGG	-	Chr2:62016713-62016732	None:intergenic	20.0%
!!	ATAATCTCAAAAGATATGAT+AGG	+	Chr2:62016731-62016750	MsG0280009967.01.T01:intron	20.0%
!!	ATATATATCATACTTTCATC+AGG	-	Chr2:62016557-62016576	None:intergenic	20.0%
!!	TATATATCATACTTTCATCA+GGG	-	Chr2:62016556-62016575	None:intergenic	20.0%
	AAAGTAAAAAATGGGAGGTA+AGG	-	Chr2:62016585-62016604	None:intergenic	30.0%
	GAAGTTATAGCTAAATCACT+TGG	+	Chr2:62016787-62016806	MsG0280009967.01.T01:CDS	30.0%
	TGAAAGGAAGAAAAATTGAG+AGG	-	Chr2:62016480-62016499	None:intergenic	30.0%
!	ATTTGTTGATATGCATCCAA+AGG	+	Chr2:62016679-62016698	MsG0280009967.01.T01:intron	30.0%
	AATCACTTGGTAAGAAGGAT+TGG	+	Chr2:62016800-62016819	MsG0280009967.01.T01:CDS	35.0%
	ACATTGTTCTCTACGACATT+CGG	-	Chr2:62016880-62016899	None:intergenic	35.0%
	ACCAAATGGAAATGAGTGAA+AGG	-	Chr2:62016496-62016515	None:intergenic	35.0%
	AGCTAAATCACTTGGTAAGA+AGG	+	Chr2:62016795-62016814	MsG0280009967.01.T01:CDS	35.0%
	TATTATGGAGTTGACACCTT+TGG	-	Chr2:62016698-62016717	None:intergenic	35.0%
	TCCTTTCACTCATTTCCATT+TGG	+	Chr2:62016492-62016511	MsG0280009967.01.T01:CDS	35.0%
	TGCAAGAGATATGAACCAAA+TGG	-	Chr2:62016510-62016529	None:intergenic	35.0%
	ATTTGTAACTGCTCTGTTGC+TGG	+	Chr2:62016901-62016920	MsG0280009967.01.T01:CDS	40.0%
	TTTGTAACTGCTCTGTTGCT+GGG	+	Chr2:62016902-62016921	MsG0280009967.01.T01:CDS	40.0%
	ATCGTAGGTGTAACCCAGTT+AGG	-	Chr2:62016856-62016875	None:intergenic	45.0%
	ATGCAGCAACCAACCTAACT+GGG	+	Chr2:62016840-62016859	MsG0280009967.01.T01:CDS	45.0%
	TAGGTGTAACCCAGTTAGGT+TGG	-	Chr2:62016852-62016871	None:intergenic	45.0%
	TCTACGACATTCGGTATCGT+AGG	-	Chr2:62016871-62016890	None:intergenic	45.0%
	TTGTAACTGCTCTGTTGCTG+GGG	+	Chr2:62016903-62016922	MsG0280009967.01.T01:CDS	45.0%
!!	ATAAAATATAAAGTAAAAAA+TGG	-	Chr2:62016594-62016613	None:intergenic	5.0%
!!	TAAAATATAAAGTAAAAAAT+GGG	-	Chr2:62016593-62016612	None:intergenic	5.0%
	CATGCAGCAACCAACCTAAC+TGG	+	Chr2:62016839-62016858	MsG0280009967.01.T01:CDS	50.0%
!!	CAGTTAGGTTGGTTGCTGCA+TGG	-	Chr2:62016841-62016860	None:intergenic	50.0%

Chromosome	Type	Strat	End	Strand	Name
Chr2	gene	62016467	62016954	62016467	ID=MsG0280009967.01;Name=MsG0280009967.01
Chr2	mRNA	62016467	62016954	62016467	ID=MsG0280009967.01.T01;Parent=MsG0280009967.01;Name=MsG0280009967.01.T01;_AED=0.48;_eAED=0.49;_QI=0\|0\|0\|1\|1\|1\|2\|0\|91
Chr2	exon	62016467	62016563	62016467	ID=MsG0280009967.01.T01:exon:34611;Parent=MsG0280009967.01.T01
Chr2	exon	62016776	62016954	62016776	ID=MsG0280009967.01.T01:exon:34612;Parent=MsG0280009967.01.T01
Chr2	CDS	62016467	62016563	62016467	ID=MsG0280009967.01.T01:cds;Parent=MsG0280009967.01.T01
Chr2	CDS	62016776	62016954	62016776	ID=MsG0280009967.01.T01:cds;Parent=MsG0280009967.01.T01

Gene Sequence	>MsG0280009967.01.T01 ATGAACACTTCCTCTCAATTTTTCTTCCTTTCACTCATTTCCATTTGGTTCATATCTCTTGCAGCTTTTGCTGCTACTACTATTCACCCTGATGAAAAGAAAGCTCTTGAAGTTATAGCTAAATCACTTGGTAAGAAGGATTGGAACTTTGACATTGATCCATGCAGCAACCAACCTAACTGGGTTACACCTACGATACCGAATGTCGTAGAGAACAATGTAATTTGTAACTGCTCTGTTGCTGGGGACAACTTCTGCCATGTTATTGAAATGTAA
Protein sequence	>MsG0280009967.01.T01 MNTSSQFFFLSLISIWFISLAAFAATTIHPDEKKALEVIAKSLGKKDWNFDIDPCSNQPNWVTPTIPNVVENNVICNCSVAGDNFCHVIEM*