AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar ZhongmuNo.1 / MsG0280010129.01

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MsG0280010129.01.T01 MTR_4g017150 69.231 78 23 1 225 302 237 313 2.62e-25 103
MsG0280010129.01.T01 MTR_5g046560 69.231 78 23 1 225 302 253 329 2.93e-25 103
MsG0280010129.01.T01 MTR_6g049240 69.231 78 23 1 225 302 314 390 6.23e-25 103
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MsG0280010129.01.T01 AT5G17620 64.103 78 26 2 225 301 253 329 9.97e-21 90.9

Find 77 sgRNAs with CRISPR-Local

Find 177 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
CCTTCTGCTCCTTTCCATTT+TGG 0.054899 2:+65084693 None:intergenic
AATATAATGGAACTGGTAAT+TGG 0.145633 2:+65083681 None:intergenic
GGATGGAAACTAAATATATT+TGG 0.185411 2:-65084091 MsG0280010129.01.T01:CDS
TCACCCCGCCCACAGGTTTC+AGG 0.206835 2:+65084633 None:intergenic
TCACACGCAGCTCTTGCTTT+TGG 0.269186 2:-65082795 MsG0280010129.01.T01:CDS
TGCTTCCATTGCTCGTGAAC+AGG 0.293959 2:-65082673 MsG0280010129.01.T01:CDS
TTGAGAAGCACCGGAACTTT+AGG 0.298097 2:-65083659 MsG0280010129.01.T01:intron
TTGAATATGACTCAAATAAT+AGG 0.305584 2:-65084009 MsG0280010129.01.T01:intron
GTTCCACAACTTCATGGATT+TGG 0.306037 2:-65083112 MsG0280010129.01.T01:CDS
CAACGCAGAAGTAGATAAAT+TGG 0.311115 2:+65084476 None:intergenic
ATGGAGGTTCCACAACTTCA+TGG 0.349767 2:-65083118 MsG0280010129.01.T01:CDS
GAACTACATCATGAACAATA+TGG 0.363844 2:-65084801 None:intergenic
TTCTTTCTCACCCCGCCCAC+AGG 0.368663 2:+65084626 None:intergenic
TCTGCTCTTGCAGCTTACTT+TGG 0.387632 2:-65084112 MsG0280010129.01.T01:intron
CTCTTGCAGCTTACTTTGGA+TGG 0.389312 2:-65084108 MsG0280010129.01.T01:intron
ATATAATGGAACTGGTAATT+GGG 0.395189 2:+65083682 None:intergenic
ACCAGCTGCCAATCGGCTAT+TGG 0.397638 2:-65083089 MsG0280010129.01.T01:CDS
CCGGTGCTTCTCAAATATAA+TGG 0.412396 2:+65083668 None:intergenic
CTTCTCAAATATAATGGAAC+TGG 0.421616 2:+65083674 None:intergenic
CAGATTCTCGGAAAAGGAAA+AGG 0.425975 2:-65084734 MsG0280010129.01.T01:CDS
ACAACTGCTGAGCAGATTCT+CGG 0.437086 2:-65084746 MsG0280010129.01.T01:CDS
CCAATGGAAGTATGTTCAAT+TGG 0.437938 2:-65083724 MsG0280010129.01.T01:CDS
GTCAACATGCTAGCAAGCAC+TGG 0.442875 2:+65084767 None:intergenic
TGTGGGCGGGGTGAGAAAGA+AGG 0.443605 2:-65084624 MsG0280010129.01.T01:CDS
ACCTCCATCATGTGCGTCCA+TGG 0.447972 2:+65083133 None:intergenic
GGTTTCAGGCTAGAAGTACT+TGG 0.452264 2:+65084647 None:intergenic
CCAATTGAACATACTTCCAT+TGG 0.454968 2:+65083724 None:intergenic
CCATCATGTGCGTCCATGGC+CGG 0.455540 2:+65083137 None:intergenic
GCTCGACTCACCTAAAGTTC+CGG 0.457755 2:+65083649 None:intergenic
ACTGACTTAAATCGCAGTCT+TGG 0.476536 2:-65082705 MsG0280010129.01.T01:CDS
AAGATTGCCAACTCAAAGTA+AGG 0.477272 2:-65083759 MsG0280010129.01.T01:CDS
CTAGCCTGAAACCTGTGGGC+GGG 0.477581 2:-65084637 MsG0280010129.01.T01:CDS
AAAGATTCCTTACTTTGAGT+TGG 0.482180 2:+65083752 None:intergenic
TCCCAAGTCTACAAGTGAAA+AGG 0.484649 2:+65084057 None:intergenic
GCTGAGCAGATTCTCGGAAA+AGG 0.497767 2:-65084740 MsG0280010129.01.T01:CDS
GTCAGACCGGACATTGGAAA+AGG 0.518245 2:-65084583 MsG0280010129.01.T01:CDS
TCCAATAGCCGATTGGCAGC+TGG 0.519058 2:+65083088 None:intergenic
CAATTGAACATACTTCCATT+GGG 0.519511 2:+65083725 None:intergenic
AGTATGTTCAATTGGTATAC+CGG 0.520744 2:-65083716 MsG0280010129.01.T01:CDS
GTGGGCGGGGTGAGAAAGAA+GGG 0.521448 2:-65084623 MsG0280010129.01.T01:CDS
TGCTTCAGTTGTGGTCAGAC+CGG 0.522068 2:-65084596 MsG0280010129.01.T01:CDS
CCATTATATTTGAGAAGCAC+CGG 0.524736 2:-65083668 MsG0280010129.01.T01:CDS
GCTTCCATTGCTCGTGAACA+GGG 0.525311 2:-65082672 MsG0280010129.01.T01:CDS
ATTTCCCTGTTCACGAGCAA+TGG 0.526623 2:+65082668 None:intergenic
CAATTGTTTATAATCTTCCC+CGG 0.529262 2:+65083176 None:intergenic
TAGCCTGAAACCTGTGGGCG+GGG 0.529776 2:-65084636 MsG0280010129.01.T01:CDS
GATTTGGACCAGCTGCCAAT+CGG 0.542185 2:-65083096 MsG0280010129.01.T01:CDS
TGTGACAGAAGAAGGCCCTT+CGG 0.550341 2:+65082758 None:intergenic
GGTCTGACCACAACTGAAGC+AGG 0.557170 2:+65084598 None:intergenic
TGGAAAGGAGCAGAAGGACA+AGG 0.568105 2:-65084687 MsG0280010129.01.T01:CDS
CCGGCCATGGACGCACATGA+TGG 0.570668 2:-65083137 MsG0280010129.01.T01:CDS
TACTTCTAGCCTGAAACCTG+TGG 0.574338 2:-65084642 MsG0280010129.01.T01:CDS
AACTAGAAGAGGGCAAGAAG+AGG 0.577900 2:-65084541 MsG0280010129.01.T01:CDS
TTGTGTAGGAAATCCGGCCA+TGG 0.578627 2:-65083150 MsG0280010129.01.T01:CDS
TTATTTGAGTCATATTCAAG+TGG 0.580777 2:+65084013 None:intergenic
GGATCTTCTGAAACTTCCGA+AGG 0.582347 2:-65082774 MsG0280010129.01.T01:CDS
GTGAATCTCTAAGATTTCGT+AGG 0.584004 2:+65082814 None:intergenic
GTAAGCTTCCAATAGCCGAT+TGG 0.588083 2:+65083081 None:intergenic
GAGGTATCTAGAACTAGAAG+AGG 0.588475 2:-65084552 MsG0280010129.01.T01:CDS
TTTGATGGTCCAAAATGGAA+AGG 0.589328 2:-65084702 MsG0280010129.01.T01:CDS
AAGGGCACCTGCTTCAGTTG+TGG 0.591217 2:-65084605 MsG0280010129.01.T01:CDS
GGTCCAAATCCATGAAGTTG+TGG 0.591876 2:+65083109 None:intergenic
AGATTCTCGGAAAAGGAAAA+GGG 0.593175 2:-65084733 MsG0280010129.01.T01:CDS
CCAAAATGGAAAGGAGCAGA+AGG 0.593566 2:-65084693 MsG0280010129.01.T01:CDS
GTTGTGGTCAGACCGGACAT+TGG 0.593983 2:-65084589 MsG0280010129.01.T01:CDS
TCAACATGCTAGCAAGCACT+GGG 0.605455 2:+65084768 None:intergenic
AAGGAATCTTTATCTCCCAA+TGG 0.607243 2:-65083740 MsG0280010129.01.T01:CDS
GATCTTCTGAAACTTCCGAA+GGG 0.608089 2:-65082773 MsG0280010129.01.T01:CDS
GCCATGGACGCACATGATGG+AGG 0.614169 2:-65083134 MsG0280010129.01.T01:CDS
TCTAGCCTGAAACCTGTGGG+CGG 0.619383 2:-65084638 MsG0280010129.01.T01:CDS
GGAAAGGAGCAGAAGGACAA+GGG 0.639328 2:-65084686 MsG0280010129.01.T01:CDS
AGGTATCTAGAACTAGAAGA+GGG 0.641356 2:-65084551 MsG0280010129.01.T01:CDS
ATTGGAAAAGGAATTGTGAG+AGG 0.646212 2:-65084571 MsG0280010129.01.T01:CDS
ACTTCTAGCCTGAAACCTGT+GGG 0.647934 2:-65084641 MsG0280010129.01.T01:CDS
TAATTGGGCTGCATAGCCAC+CGG 0.651531 2:+65083697 None:intergenic
ATGTTCAATTGGTATACCGG+TGG 0.657299 2:-65083713 MsG0280010129.01.T01:CDS
ACACATGCGCATAAGATCCG+GGG 0.693749 2:-65083193 MsG0280010129.01.T01:intron

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!! AAGATTATTATTCAATAACA+TGG + Chr2:65084551-65084570 None:intergenic 15.0%
!! TTTGAATATGCTAATATTAA+TGG + Chr2:65083892-65083911 None:intergenic 15.0%
!! GTGCATTATATAATCATATT+TGG - Chr2:65083571-65083590 MsG0280010129.01.T01:intron 20.0%
!! TTGAATATGACTCAAATAAT+AGG - Chr2:65083435-65083454 MsG0280010129.01.T01:intron 20.0%
! CAGAATATTATCAGTACTTA+GGG + Chr2:65083316-65083335 None:intergenic 25.0%
! GAATATGCTAATATTAATGG+AGG + Chr2:65083889-65083908 None:intergenic 25.0%
! GGATGGAAACTAAATATATT+TGG - Chr2:65083353-65083372 MsG0280010129.01.T01:intron 25.0%
! TCTAGTATCTAAAAAGTAGT+AGG - Chr2:65083034-65083053 MsG0280010129.01.T01:intron 25.0%
! TCTCCTTCAATAGTTATTTA+TGG + Chr2:65084075-65084094 None:intergenic 25.0%
! TTATTTGAGTCATATTCAAG+TGG + Chr2:65083434-65083453 None:intergenic 25.0%
! TTGCCATAAATAACTATTGA+AGG - Chr2:65084069-65084088 MsG0280010129.01.T01:CDS 25.0%
!! AATATAATGGAACTGGTAAT+TGG + Chr2:65083766-65083785 None:intergenic 25.0%
!! ATATAATGGAACTGGTAATT+GGG + Chr2:65083765-65083784 None:intergenic 25.0%
!!! AATAATCTTGTTGATTTCGA+TGG - Chr2:65084562-65084581 MsG0280010129.01.T01:CDS 25.0%
!!! ATAAACAATTGCTTTTGTGT+AGG - Chr2:65084280-65084299 MsG0280010129.01.T01:intron 25.0%
!!! ATTATTTTTGTGACAGAAGA+AGG + Chr2:65084697-65084716 None:intergenic 25.0%
!!! TACTTTTTTGTGTGATTACT+TGG - Chr2:65084117-65084136 MsG0280010129.01.T01:intron 25.0%
AAAGATTCCTTACTTTGAGT+TGG + Chr2:65083695-65083714 None:intergenic 30.0%
AACATTGCTCAATTTCTGTA+AGG + Chr2:65084448-65084467 None:intergenic 30.0%
AGTATGTTCAATTGGTATAC+CGG - Chr2:65083728-65083747 MsG0280010129.01.T01:CDS 30.0%
CAAACTGAGATGAAATATCT+TGG - Chr2:65083639-65083658 MsG0280010129.01.T01:intron 30.0%
CAATTGAACATACTTCCATT+GGG + Chr2:65083722-65083741 None:intergenic 30.0%
CAATTGTTTATAATCTTCCC+CGG + Chr2:65084271-65084290 None:intergenic 30.0%
GACAACATAGAATAGTTGTA+GGG - Chr2:65083170-65083189 MsG0280010129.01.T01:CDS 30.0%
GCAGAATATTATCAGTACTT+AGG + Chr2:65083317-65083336 None:intergenic 30.0%
TGACAACATAGAATAGTTGT+AGG - Chr2:65083169-65083188 MsG0280010129.01.T01:CDS 30.0%
TGAGTATTCCATACTACATA+TGG - Chr2:65082991-65083010 MsG0280010129.01.T01:intron 30.0%
TTGAGCAATGTTTGATAGAA+AGG + Chr2:65084477-65084496 None:intergenic 30.0%
! AGCTTACAAAATGCTTTTGA+AGG - Chr2:65084379-65084398 MsG0280010129.01.T01:intron 30.0%
! CTTCTCAAATATAATGGAAC+TGG + Chr2:65083773-65083792 None:intergenic 30.0%
! GTAGTGTTTTGTACTGTTTA+AGG + Chr2:65084100-65084119 None:intergenic 30.0%
! GTCAATTTTCATCCATTCAT+GGG + Chr2:65083291-65083310 None:intergenic 30.0%
! TCAATTTTCATCCATTCATG+GGG + Chr2:65083290-65083309 None:intergenic 30.0%
! TGTCAATTTTCATCCATTCA+TGG + Chr2:65083292-65083311 None:intergenic 30.0%
!! AGTTTGTTTGCAACAGTTTT+TGG - Chr2:65084603-65084622 MsG0280010129.01.T01:CDS 30.0%
AAGATTGCCAACTCAAAGTA+AGG - Chr2:65083685-65083704 MsG0280010129.01.T01:CDS 35.0%
AAGGAATCTTTATCTCCCAA+TGG - Chr2:65083704-65083723 MsG0280010129.01.T01:CDS 35.0%
AGATTCTCGGAAAAGGAAAA+GGG - Chr2:65082711-65082730 MsG0280010129.01.T01:CDS 35.0%
AGTAGTAGGACGTTGTATAA+AGG - Chr2:65083048-65083067 MsG0280010129.01.T01:intron 35.0%
ATAGATATGTCCTAGCATCA+AGG + Chr2:65083964-65083983 None:intergenic 35.0%
ATTGGAAAAGGAATTGTGAG+AGG - Chr2:65082873-65082892 MsG0280010129.01.T01:intron 35.0%
CAACGCAGAAGTAGATAAAT+TGG + Chr2:65082971-65082990 None:intergenic 35.0%
CATAAATTGTGCTGAAGAAG+AGG - Chr2:65083932-65083951 MsG0280010129.01.T01:intron 35.0%
CCAATTGAACATACTTCCAT+TGG + Chr2:65083723-65083742 None:intergenic 35.0%
CTCTTCTTCAGCACAATTTA+TGG + Chr2:65083934-65083953 None:intergenic 35.0%
GGAAATGTGAGTACTTTCTT+GGG - Chr2:65083660-65083679 MsG0280010129.01.T01:CDS 35.0%
TAGATATGTCCTAGCATCAA+GGG + Chr2:65083963-65083982 None:intergenic 35.0%
TGGAAATGTGAGTACTTTCT+TGG - Chr2:65083659-65083678 MsG0280010129.01.T01:intron 35.0%
TGTGAGATCCATATGTAGTA+TGG + Chr2:65083002-65083021 None:intergenic 35.0%
! AAAAGGGAAGACCATTTTGA+TGG - Chr2:65082727-65082746 MsG0280010129.01.T01:CDS 35.0%
! ACAATTCCTTTTCCAATGTC+CGG + Chr2:65082870-65082889 None:intergenic 35.0%
! AGGTATCTAGAACTAGAAGA+GGG - Chr2:65082893-65082912 MsG0280010129.01.T01:intron 35.0%
! ATCCTTTTCACTTGTAGACT+TGG - Chr2:65083385-65083404 MsG0280010129.01.T01:intron 35.0%
! CATTTTTCTCTACGATGACA+GGG + Chr2:65083227-65083246 None:intergenic 35.0%
! CCAATGGAAGTATGTTCAAT+TGG - Chr2:65083720-65083739 MsG0280010129.01.T01:CDS 35.0%
! CCATTATATTTGAGAAGCAC+CGG - Chr2:65083776-65083795 MsG0280010129.01.T01:intron 35.0%
! GTTGACTTACGAGTTTGAAA+TGG - Chr2:65083075-65083094 MsG0280010129.01.T01:CDS 35.0%
! TCCATTTTGGACCATCAAAA+TGG + Chr2:65082741-65082760 None:intergenic 35.0%
! TCCTTTTCACTTGTAGACTT+GGG - Chr2:65083386-65083405 MsG0280010129.01.T01:intron 35.0%
! TTTGATGGTCCAAAATGGAA+AGG - Chr2:65082742-65082761 MsG0280010129.01.T01:CDS 35.0%
!! ACCATTTTGATGGTCCAAAA+TGG - Chr2:65082737-65082756 MsG0280010129.01.T01:CDS 35.0%
!! GTGAATCTCTAAGATTTCGT+AGG + Chr2:65084633-65084652 None:intergenic 35.0%
!! TGGTTAGAAAAGGCACTATA+CGG - Chr2:65083470-65083489 MsG0280010129.01.T01:intron 35.0%
!! TTGCTTTTGTGTAGGAAATC+CGG - Chr2:65084288-65084307 MsG0280010129.01.T01:intron 35.0%
AGGACATATCTATGCAGCAA+AGG - Chr2:65083971-65083990 MsG0280010129.01.T01:intron 40.0%
ATAATAGGTACCAAGTGTGC+TGG - Chr2:65083450-65083469 MsG0280010129.01.T01:intron 40.0%
ATCTTATGCGCATGTGTTAG+GGG + Chr2:65084248-65084267 None:intergenic 40.0%
ATGTTCAATTGGTATACCGG+TGG - Chr2:65083731-65083750 MsG0280010129.01.T01:CDS 40.0%
CAAACAGGGAAGTTCCTATA+GGG - Chr2:65083262-65083281 MsG0280010129.01.T01:intron 40.0%
CAGATTCTCGGAAAAGGAAA+AGG - Chr2:65082710-65082729 MsG0280010129.01.T01:CDS 40.0%
CCGGTGCTTCTCAAATATAA+TGG + Chr2:65083779-65083798 None:intergenic 40.0%
CTATAGGAACTTCCCTGTTT+GGG + Chr2:65083263-65083282 None:intergenic 40.0%
GATCTTATGCGCATGTGTTA+GGG + Chr2:65084249-65084268 None:intergenic 40.0%
GTTCCACAACTTCATGGATT+TGG - Chr2:65084332-65084351 MsG0280010129.01.T01:intron 40.0%
TAAAAATTGGGACTGGGAGA+TGG + Chr2:65083124-65083143 None:intergenic 40.0%
TAACACATGCGCATAAGATC+CGG - Chr2:65084249-65084268 MsG0280010129.01.T01:intron 40.0%
TAGAGAAAAATGCCTGCTGT+GGG - Chr2:65083235-65083254 MsG0280010129.01.T01:intron 40.0%
TATAATGCACATCACTGCCA+TGG + Chr2:65083561-65083580 None:intergenic 40.0%
TCCCAAGTCTACAAGTGAAA+AGG + Chr2:65083390-65083409 None:intergenic 40.0%
! ACCAAGAGTGTGATGTCATT+GGG - Chr2:65083523-65083542 MsG0280010129.01.T01:intron 40.0%
! ACTGACTTAAATCGCAGTCT+TGG - Chr2:65084739-65084758 MsG0280010129.01.T01:CDS 40.0%
! ATCTCCCAGTCCCAATTTTT+AGG - Chr2:65083123-65083142 MsG0280010129.01.T01:CDS 40.0%
! GAGGTATCTAGAACTAGAAG+AGG - Chr2:65082892-65082911 MsG0280010129.01.T01:intron 40.0%
! GAGTTTAGTTTAGCGAAGCT+AGG + Chr2:65083831-65083850 None:intergenic 40.0%
! GCATTTTTCTCTACGATGAC+AGG + Chr2:65083228-65083247 None:intergenic 40.0%
! TTTAAGATGTGAGCCAAGCA+TGG + Chr2:65084413-65084432 None:intergenic 40.0%
!! GATCTTCTGAAACTTCCGAA+GGG - Chr2:65084671-65084690 MsG0280010129.01.T01:CDS 40.0%
AACACATGCGCATAAGATCC+GGG - Chr2:65084250-65084269 MsG0280010129.01.T01:intron 45.0%
AACTAGAAGAGGGCAAGAAG+AGG - Chr2:65082903-65082922 MsG0280010129.01.T01:intron 45.0%
ACAACTGCTGAGCAGATTCT+CGG - Chr2:65082698-65082717 MsG0280010129.01.T01:CDS 45.0%
ACTAGTCATGCGAGATTGCA+TGG - Chr2:65084163-65084182 MsG0280010129.01.T01:intron 45.0%
ACTTCTAGCCTGAAACCTGT+GGG - Chr2:65082803-65082822 MsG0280010129.01.T01:CDS 45.0%
AGAGAAAAATGCCTGCTGTG+GGG - Chr2:65083236-65083255 MsG0280010129.01.T01:intron 45.0%
AGAGGAGCCGACCTAAAAAT+TGG + Chr2:65083137-65083156 None:intergenic 45.0%
ATGGAGGTTCCACAACTTCA+TGG - Chr2:65084326-65084345 MsG0280010129.01.T01:intron 45.0%
ATTTCCCTGTTCACGAGCAA+TGG + Chr2:65084779-65084798 None:intergenic 45.0%
CCAAAATGGAAAGGAGCAGA+AGG - Chr2:65082751-65082770 MsG0280010129.01.T01:CDS 45.0%
CCAAACAGGGAAGTTCCTAT+AGG - Chr2:65083261-65083280 MsG0280010129.01.T01:intron 45.0%
CCGACCTAAAAATTGGGACT+GGG + Chr2:65083130-65083149 None:intergenic 45.0%
CCTATAGGAACTTCCCTGTT+TGG + Chr2:65083264-65083283 None:intergenic 45.0%
CTCTTGCAGCTTACTTTGGA+TGG - Chr2:65083336-65083355 MsG0280010129.01.T01:intron 45.0%
GAAGGTAACCTTTCCATGCT+TGG - Chr2:65084397-65084416 MsG0280010129.01.T01:intron 45.0%
GAGGAGCCGACCTAAAAATT+GGG + Chr2:65083136-65083155 None:intergenic 45.0%
GGATCTTATGCGCATGTGTT+AGG + Chr2:65084250-65084269 None:intergenic 45.0%
GGTCCAAATCCATGAAGTTG+TGG + Chr2:65084338-65084357 None:intergenic 45.0%
GTAAGCTTCCAATAGCCGAT+TGG + Chr2:65084366-65084385 None:intergenic 45.0%
GTAGAGAAAAATGCCTGCTG+TGG - Chr2:65083234-65083253 MsG0280010129.01.T01:intron 45.0%
TACTTCTAGCCTGAAACCTG+TGG - Chr2:65082802-65082821 MsG0280010129.01.T01:CDS 45.0%
TCAACATGCTAGCAAGCACT+GGG + Chr2:65082679-65082698 None:intergenic 45.0%
TCCCAATGACATCACACTCT+TGG + Chr2:65083527-65083546 None:intergenic 45.0%
TCTGCTCTTGCAGCTTACTT+TGG - Chr2:65083332-65083351 MsG0280010129.01.T01:intron 45.0%
TGAAGCAATCCTCTCTTGCT+TGG - Chr2:65083495-65083514 MsG0280010129.01.T01:intron 45.0%
TGGTGAAAACCAAGCAAGAG+AGG + Chr2:65083507-65083526 None:intergenic 45.0%
TTGGGATTTAGACAGAGCCA+TGG - Chr2:65083541-65083560 MsG0280010129.01.T01:intron 45.0%
! CACCAAGAGTGTGATGTCAT+TGG - Chr2:65083522-65083541 MsG0280010129.01.T01:intron 45.0%
! CCTTCTGCTCCTTTCCATTT+TGG + Chr2:65082754-65082773 None:intergenic 45.0%
! CCTTTTCTAACCAGCACACT+TGG + Chr2:65083463-65083482 None:intergenic 45.0%
!! CCAAGTGTGCTGGTTAGAAA+AGG - Chr2:65083460-65083479 MsG0280010129.01.T01:intron 45.0%
!! GGATCTTCTGAAACTTCCGA+AGG - Chr2:65084670-65084689 MsG0280010129.01.T01:CDS 45.0%
!! GGTTTCAGGCTAGAAGTACT+TGG + Chr2:65082800-65082819 None:intergenic 45.0%
!! TTGAGAAGCACCGGAACTTT+AGG - Chr2:65083785-65083804 MsG0280010129.01.T01:intron 45.0%
!!! CCCAGTCCCAATTTTTAGGT+CGG - Chr2:65083127-65083146 MsG0280010129.01.T01:CDS 45.0%
ACACATGCGCATAAGATCCG+GGG - Chr2:65084251-65084270 MsG0280010129.01.T01:intron 50.0%
AGGTGAGTCGAGCTTGAGTA+AGG - Chr2:65083805-65083824 MsG0280010129.01.T01:intron 50.0%
ATGGAGGAGTGAATTCCACG+TGG + Chr2:65083873-65083892 None:intergenic 50.0%
CCATTCATGGGGATCCCTAT+AGG + Chr2:65083279-65083298 None:intergenic 50.0%
CCTATAGGGATCCCCATGAA+TGG - Chr2:65083276-65083295 MsG0280010129.01.T01:intron 50.0%
GATGTGAGCCAAGCATGGAA+AGG + Chr2:65084408-65084427 None:intergenic 50.0%
GATTTGGACCAGCTGCCAAT+CGG - Chr2:65084348-65084367 MsG0280010129.01.T01:intron 50.0%
GCCGACCTAAAAATTGGGAC+TGG + Chr2:65083131-65083150 None:intergenic 50.0%
GCTCGACTCACCTAAAGTTC+CGG + Chr2:65083798-65083817 None:intergenic 50.0%
GCTGAGCAGATTCTCGGAAA+AGG - Chr2:65082704-65082723 MsG0280010129.01.T01:CDS 50.0%
GCTTCCATTGCTCGTGAACA+GGG - Chr2:65084772-65084791 MsG0280010129.01.T01:CDS 50.0%
GGAAAGGAGCAGAAGGACAA+GGG - Chr2:65082758-65082777 MsG0280010129.01.T01:CDS 50.0%
GTCAACATGCTAGCAAGCAC+TGG + Chr2:65082680-65082699 None:intergenic 50.0%
GTCAGACCGGACATTGGAAA+AGG - Chr2:65082861-65082880 MsG0280010129.01.T01:intron 50.0%
TAATTGGGCTGCATAGCCAC+CGG + Chr2:65083750-65083769 None:intergenic 50.0%
TCTCTACGATGACAGGGACT+GGG + Chr2:65083221-65083240 None:intergenic 50.0%
TGCTTCAGTTGTGGTCAGAC+CGG - Chr2:65082848-65082867 MsG0280010129.01.T01:intron 50.0%
TGCTTCCATTGCTCGTGAAC+AGG - Chr2:65084771-65084790 MsG0280010129.01.T01:CDS 50.0%
TGGAAAGGAGCAGAAGGACA+AGG - Chr2:65082757-65082776 MsG0280010129.01.T01:CDS 50.0%
TGTGACAGAAGAAGGCCCTT+CGG + Chr2:65084689-65084708 None:intergenic 50.0%
TTCTCTACGATGACAGGGAC+TGG + Chr2:65083222-65083241 None:intergenic 50.0%
TTGTCAGTCCCAGATTGCAG+AGG + Chr2:65083155-65083174 None:intergenic 50.0%
TTGTGTAGGAAATCCGGCCA+TGG - Chr2:65084294-65084313 MsG0280010129.01.T01:intron 50.0%
! TCACACGCAGCTCTTGCTTT+TGG - Chr2:65084649-65084668 MsG0280010129.01.T01:CDS 50.0%
ACCAGCTGCCAATCGGCTAT+TGG - Chr2:65084355-65084374 MsG0280010129.01.T01:intron 55.0%
ACCTCCATCATGTGCGTCCA+TGG + Chr2:65084314-65084333 None:intergenic 55.0%
ATGCGCATGTGTTAGGGGCA+CGG + Chr2:65084243-65084262 None:intergenic 55.0%
GAGGAAGAGCCCTTGATGCT+AGG - Chr2:65083951-65083970 MsG0280010129.01.T01:intron 55.0%
GAGTGAATTCCACGTGGTGC+TGG + Chr2:65083867-65083886 None:intergenic 55.0%
GGTCTGACCACAACTGAAGC+AGG + Chr2:65082849-65082868 None:intergenic 55.0%
GTTGTGGTCAGACCGGACAT+TGG - Chr2:65082855-65082874 MsG0280010129.01.T01:intron 55.0%
TCCAATAGCCGATTGGCAGC+TGG + Chr2:65084359-65084378 None:intergenic 55.0%
TCTAGCCTGAAACCTGTGGG+CGG - Chr2:65082806-65082825 MsG0280010129.01.T01:CDS 55.0%
!! AAGGGCACCTGCTTCAGTTG+TGG - Chr2:65082839-65082858 MsG0280010129.01.T01:CDS 55.0%
!! AATTCCACGTGGTGCTGGTC+AGG + Chr2:65083862-65083881 None:intergenic 55.0%
!! ATTCCACGTGGTGCTGGTCA+GGG + Chr2:65083861-65083880 None:intergenic 55.0%
CCATCATGTGCGTCCATGGC+CGG + Chr2:65084310-65084329 None:intergenic 60.0%
CGATGACAGGGACTGGGAGA+AGG + Chr2:65083215-65083234 None:intergenic 60.0%
CTAGCCTGAAACCTGTGGGC+GGG - Chr2:65082807-65082826 MsG0280010129.01.T01:CDS 60.0%
CTGCTGTGGGGTCCCAAACA+GGG - Chr2:65083248-65083267 MsG0280010129.01.T01:intron 60.0%
GCCATGGACGCACATGATGG+AGG - Chr2:65084310-65084329 MsG0280010129.01.T01:intron 60.0%
GTGGGCGGGGTGAGAAAGAA+GGG - Chr2:65082821-65082840 MsG0280010129.01.T01:CDS 60.0%
TAGCCTGAAACCTGTGGGCG+GGG - Chr2:65082808-65082827 MsG0280010129.01.T01:CDS 60.0%
TGTGGGCGGGGTGAGAAAGA+AGG - Chr2:65082820-65082839 MsG0280010129.01.T01:CDS 60.0%
TTCTTTCTCACCCCGCCCAC+AGG + Chr2:65082821-65082840 None:intergenic 60.0%
! AGGTCGGCTCCTCTGCAATC+TGG - Chr2:65083143-65083162 MsG0280010129.01.T01:CDS 60.0%
! GCATGTGTTAGGGGCACGGT+GGG + Chr2:65084239-65084258 None:intergenic 60.0%
! GGTCGGCTCCTCTGCAATCT+GGG - Chr2:65083144-65083163 MsG0280010129.01.T01:CDS 60.0%
CCGGCCATGGACGCACATGA+TGG - Chr2:65084307-65084326 MsG0280010129.01.T01:intron 65.0%
CCTGCTGTGGGGTCCCAAAC+AGG - Chr2:65083247-65083266 MsG0280010129.01.T01:intron 65.0%
TCACCCCGCCCACAGGTTTC+AGG + Chr2:65082814-65082833 None:intergenic 65.0%
! CCTGTTTGGGACCCCACAGC+AGG + Chr2:65083250-65083269 None:intergenic 65.0%
! CGCATGTGTTAGGGGCACGG+TGG + Chr2:65084240-65084259 None:intergenic 65.0%
CCACCCTGACCAGCACCACG+TGG - Chr2:65083855-65083874 MsG0280010129.01.T01:intron 70.0%
!! CCACGTGGTGCTGGTCAGGG+TGG + Chr2:65083858-65083877 None:intergenic 70.0%
Chromosome Type Strat End Strand Name
Chr2 gene 65082653 65084813 65082653 ID=MsG0280010129.01;Name=MsG0280010129.01
Chr2 mRNA 65082653 65084813 65082653 ID=MsG0280010129.01.T01;Parent=MsG0280010129.01;Name=MsG0280010129.01.T01;_AED=0.47;_eAED=0.72;_QI=0|0|0|1|0.75|0.6|5|0|303
Chr2 exon 65084477 65084813 65084477 ID=MsG0280010129.01.T01:exon:14102;Parent=MsG0280010129.01.T01
Chr2 exon 65084010 65084121 65084010 ID=MsG0280010129.01.T01:exon:14101;Parent=MsG0280010129.01.T01
Chr2 exon 65083660 65083778 65083660 ID=MsG0280010129.01.T01:exon:14100;Parent=MsG0280010129.01.T01
Chr2 exon 65083066 65083200 65083066 ID=MsG0280010129.01.T01:exon:14099;Parent=MsG0280010129.01.T01
Chr2 exon 65082653 65082861 65082653 ID=MsG0280010129.01.T01:exon:14098;Parent=MsG0280010129.01.T01
Chr2 CDS 65084477 65084813 65084477 ID=MsG0280010129.01.T01:cds;Parent=MsG0280010129.01.T01
Chr2 CDS 65084010 65084121 65084010 ID=MsG0280010129.01.T01:cds;Parent=MsG0280010129.01.T01
Chr2 CDS 65083660 65083778 65083660 ID=MsG0280010129.01.T01:cds;Parent=MsG0280010129.01.T01
Chr2 CDS 65083066 65083200 65083066 ID=MsG0280010129.01.T01:cds;Parent=MsG0280010129.01.T01
Chr2 CDS 65082653 65082861 65082653 ID=MsG0280010129.01.T01:cds;Parent=MsG0280010129.01.T01
Gene Sequence

>MsG0280010129.01.T01

ATGAACAATATGGACAAGAGTCTCCCAGTGCTTGCTAGCATGTTGACAACTGCTGAGCAGATTCTCGGAAAAGGAAAAGGGAAGACCATTTTGATGGTCCAAAATGGAAAGGAGCAGAAGGACAAGGGCAAGAAGAAGTTTAAGCCAAGTACTTCTAGCCTGAAACCTGTGGGCGGGGTGAGAAAGAAGGGCACCTGCTTCAGTTGTGGTCAGACCGGACATTGGAAAAGGAATTGTGAGAGGTATCTAGAACTAGAAGAGGGCAAGAAGAGGAAAGTAAGTGATGCTTCTATTAAGTTATATTTATTTCAGAAGCCAATTTATCTACTTCTGCGTTCTTACTTTGGATGGAAACTAAATATATTTGGTTTTAAAACATCCTTTTCACTTGTAGACTTGGGAAATGAAGTATTCATAGAGATTCCACTTGAATATGACTCAAATAATAGATTGCCAACTCAAAGTAAGGAATCTTTATCTCCCAATGGAAGTATGTTCAATTGGTATACCGGTGGCTATGCAGCCCAATTACCAGTTCCATTATATTTGAGAAGCACCGGAACTTTAGATCCGGGGAAGATTATAAACAATTGCTTTTGTGTAGGAAATCCGGCCATGGACGCACATGATGGAGGTTCCACAACTTCATGGATTTGGACCAGCTGCCAATCGGCTATTGGAAGCTTACAAAATGCTTTTGAAGTTTGTTTGCAACAGTTTTTGGAGAACCTACGAAATCTTAGAGATTCACACGCAGCTCTTGCTTTTGGATCTTCTGAAACTTCCGAAGGGCCTTCTTCTGTCACAAAAATAATGTCTGAATGTGAATCTGCATTGACTGACTTAAATCGCAGTCTTGGAATTCTTTCTGCTTCCATTGCTCGTGAACAGGGAAATAAGATGAGCACTTGA
Protein sequence

>MsG0280010129.01.T01

MNNMDKSLPVLASMLTTAEQILGKGKGKTILMVQNGKEQKDKGKKKFKPSTSSLKPVGGVRKKGTCFSCGQTGHWKRNCERYLELEEGKKRKVSDASIKLYLFQKPIYLLLRSYFGWKLNIFGFKTSFSLVDLGNEVFIEIPLEYDSNNRLPTQSKESLSPNGSMFNWYTGGYAAQLPVPLYLRSTGTLDPGKIINNCFCVGNPAMDAHDGGSTTSWIWTSCQSAIGSLQNAFEVCLQQFLENLRNLRDSHAALAFGSSETSEGPSSVTKIMSECESALTDLNRSLGILSASIAREQGNKMST*