AlfalfaGEDB | MsG0280010136.01

M. sativa cultivar ZhongmuNo.1 / MsG0280010136.01

KEGG: K05580
GO: GO:0016020 GO:0016651 GO:0051539 GO:0055114
PFAM: PF13187

Nr:

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Query id	Subject id	identity %	alignment length	mis matches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MsG0280010136.01.T01	QVX28521.1	98.374	123	2	0	5	127	39	161	1.35E-84	254

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Swissprot:

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Query id	Subject id	identity %	alignment length	mis matches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MsG0280010136.01.T01	Q9BBP0	97.561	123	3	0	5	127	39	161	2.92E-86	251

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Trembl:

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Query id	Subject id	identity %	alignment length	mis matches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MsG0280010136.01.T01	A0A6H0EHW3	99.187	123	1	0	5	127	39	161	7.07e-85	254

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TFs/TRs:

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Gene ID	Type	Classification
No data available in table

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Protein Kinases:

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Gene ID	Type	Classification
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Network:

Co-expression Network

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Gene1	Gene2	correlation coefficient	p_value	FDR
MsG0280010136.01	MsG0380014013.01	0.801630	8.223603e-49	3.946271e-46
MsG0280010136.01	MsG0380014022.01	0.800064	1.717214e-48	7.920377e-46

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PPI

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Gene1	Gene2	Type
No data available in table

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Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MsG0280010136.01.T01	MTR_6g042210	99.187	123	1	0	5	127	39	161	2.58e-88	253
MsG0280010136.01.T01	MTR_3g450170	99.187	123	1	0	5	127	39	161	2.58e-88	253
MsG0280010136.01.T01	MTR_7g021700	99.187	123	1	0	5	127	513	635	2.78e-85	261
MsG0280010136.01.T01	MTR_4g006100	99.187	123	1	0	5	127	777	899	6.89e-83	260
MsG0280010136.01.T01	MTR_5g015855	99.138	116	1	0	12	127	30	145	1.64e-82	238
MsG0280010136.01.T01	MTR_0002s0160	90.909	121	11	0	5	125	41	161	6.82e-80	236
MsG0280010136.01.T01	MTR_3g054410	96.939	98	3	0	5	102	36	133	2.53e-67	200
MsG0280010136.01.T01	MTR_5g063400	97.938	97	2	0	31	127	59	155	1.00e-66	199
MsG0280010136.01.T01	MTR_4g050810	78.049	123	1	1	5	127	236	332	7.58e-61	190
MsG0280010136.01.T01	MTR_8g086030	88.679	53	6	0	58	110	60	112	1.12e-29	103

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Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MsG0280010136.01.T01	ATCG01090	96.667	120	4	0	5	124	39	158	2.05e-84	244
MsG0280010136.01.T01	AT1G16700	35.780	109	64	2	5	108	93	200	1.85e-14	67.4
MsG0280010136.01.T01	AT1G79010	35.780	109	64	2	5	108	93	200	2.16e-14	67.0

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Find 17 sgRNAs with CRISPR-Local

Find 20 sgRNAs with CRISPR-GE

CRISPR-Local

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CRISPR-Local
sgRNA_sequence	on_target_score	Position	Region
AACTATAATCAAATTGCTTT+GGG	0.280375	2:+65286714	MsG0280010136.01.T01:CDS
ACCCGTTGTTGATTGGAAAC+TGG	0.369567	2:+65286542	MsG0280010136.01.T01:CDS
ATCACATCGGAACGTTTCCG+CGG	0.597937	2:+65286450	MsG0280010136.01.T01:CDS
ATTCAAAATGGATTCGACCG+CGG	0.695705	2:-65286467	None:intergenic
CAATTACTGACATTGGTAAA+CGG	0.469669	2:-65286737	None:intergenic
CATAAGGATATTGAATAGTG+GGG	0.595300	2:-65286419	None:intergenic
GAACTATAATCAAATTGCTT+TGG	0.315151	2:+65286713	MsG0280010136.01.T01:CDS
GTCATTGATAAACAATTTGT+TGG	0.381894	2:-65286651	None:intergenic
TAATCATCAATTACTGACAT+TGG	0.558627	2:-65286744	None:intergenic
TAGATTTACCCGTTGTTGAT+TGG	0.366477	2:+65286535	MsG0280010136.01.T01:CDS

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CRISPR-GE

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badsite warning	sgRNA_sequence	Strand	Position	Region	GC_content
	CAATTACTGACATTGGTAAA+CGG	-	Chr2:65286740-65286759	None:intergenic	30.0%
	CATAAGGATATTGAATAGTG+GGG	-	Chr2:65286422-65286441	None:intergenic	30.0%
	TAGATTTACCCGTTGTTGAT+TGG	+	Chr2:65286535-65286554	MsG0280010136.01.T01:CDS	35.0%
	TCAACAACGGGTAAATCTAT+AGG	-	Chr2:65286534-65286553	None:intergenic	35.0%
	TTCCAGTTTCCAATCAACAA+CGG	-	Chr2:65286547-65286566	None:intergenic	35.0%
	TCCAGTTTCCAATCAACAAC+GGG	-	Chr2:65286546-65286565	None:intergenic	40.0%
	ATCACATCGGAACGTTTCCG+CGG	+	Chr2:65286450-65286469	MsG0280010136.01.T01:CDS	50.0%
!	GAACTATAATCAAATTGCTT+TGG	+	Chr2:65286713-65286732	MsG0280010136.01.T01:CDS	25.0%
!	GTCATTGATAAACAATTTGT+TGG	-	Chr2:65286654-65286673	None:intergenic	25.0%
!	TAATCATCAATTACTGACAT+TGG	-	Chr2:65286747-65286766	None:intergenic	25.0%

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Chromosome	Type	Strat	End	Strand	Name
Chr2	gene	65286411	65286794	65286411	ID=MsG0280010136.01;Name=MsG0280010136.01
Chr2	mRNA	65286411	65286794	65286411	ID=MsG0280010136.01.T01;Parent=MsG0280010136.01;Name=MsG0280010136.01.T01;_AED=0.48;_eAED=0.48;_QI=0\|-1\|0\|1\|-1\|1\|1\|0\|127
Chr2	exon	65286411	65286794	65286411	ID=MsG0280010136.01.T01:exon:18017;Parent=MsG0280010136.01.T01
Chr2	CDS	65286411	65286794	65286411	ID=MsG0280010136.01.T01:cds;Parent=MsG0280010136.01.T01

Gene Sequence

>MsG0280010136.01.T01

ATGAACGTCCCCACTATTCAATATCCTTATGAAAAATTAATCACATCGGAACGTTTCCGCGGTCGAATCCATTTTGAATTTGATAAATGCATTGCTTGTGAAGTATGTGTTCGTGTATGTCCTATAGATTTACCCGTTGTTGATTGGAAACTGGAAACTGATATTCGAAAGAAACGATTGCTAAATTACAGTATTGATTTCAGAATCTGTATTTTTTGTGGTAACTGTATTGAGTATTGCCCAACAAATTGTTTATCAATGACTGAAGAATATGAACTTTCAACTTATGATCGTCACGAATTGAACTATAATCAAATTGCTTTGGGCCGTTTACCAATGTCAGTAATTGATGATTATACAATTCGAACAATTCAAATAAAATAA

Protein sequence

>MsG0280010136.01.T01

MNVPTIQYPYEKLITSERFRGRIHFEFDKCIACEVCVRVCPIDLPVVDWKLETDIRKKRLLNYSIDFRICIFCGNCIEYCPTNCLSMTEEYELSTYDRHELNYNQIALGRLPMSVIDDYTIRTIQIK*