Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MsG0380014013.01.T01 | YP_009141619.1 | 100 | 61 | 0 | 0 | 1 | 61 | 1 | 61 | 2.21E-33 | 118 |
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MsG0380014013.01.T01 | Q49L15 | 93.443 | 61 | 4 | 0 | 1 | 61 | 1 | 61 | 2.30E-34 | 113 |
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MsG0380014013.01.T01 | A0A0K0LTP3 | 100.000 | 61 | 0 | 0 | 1 | 61 | 1 | 61 | 1.05e-33 | 118 |
| Gene ID | Type | Classification |
|---|
| Gene ID | Type | Classification |
|---|
Co-expression Network
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|---|---|---|---|
| MsG0080047910.01 | MsG0380014013.01 | 0.814059 | 1.868017e-51 | 1.240613e-48 |
| MsG0080048611.01 | MsG0380014013.01 | 0.840821 | 6.902948e-58 | 9.950497e-55 |
| MsG0080048689.01 | MsG0380014013.01 | 0.820803 | 5.654880e-53 | 4.520071e-50 |
| MsG0080048929.01 | MsG0380014013.01 | 0.812390 | 4.343034e-51 | 2.757929e-48 |
| MsG0080049078.01 | MsG0380014013.01 | 0.882008 | 1.492452e-70 | 8.939924e-67 |
| MsG0080049079.01 | MsG0380014013.01 | 0.878602 | 2.460090e-69 | 1.292545e-65 |
| MsG0080049080.01 | MsG0380014013.01 | 0.826385 | 2.792637e-54 | 2.615163e-51 |
| MsG0080049129.01 | MsG0380014013.01 | 0.820280 | 7.456995e-53 | 5.874520e-50 |
| MsG0180000325.01 | MsG0380014013.01 | 0.836237 | 1.052396e-56 | 1.318491e-53 |
| MsG0180000502.01 | MsG0380014013.01 | 0.803568 | 3.276750e-49 | 1.652252e-46 |
| MsG0180001249.01 | MsG0380014013.01 | 0.811773 | 5.919375e-51 | 3.696700e-48 |
| MsG0180003274.01 | MsG0380014013.01 | 0.810182 | 1.309045e-50 | 7.837493e-48 |
| MsG0180003442.01 | MsG0380014013.01 | 0.805720 | 1.164828e-49 | 6.207795e-47 |
| MsG0180003933.01 | MsG0380014013.01 | 0.863226 | 2.895121e-64 | 8.713092e-61 |
| MsG0180004188.01 | MsG0380014013.01 | 0.805855 | 1.091124e-49 | 5.835136e-47 |
| MsG0180004302.01 | MsG0380014013.01 | 0.829390 | 5.288927e-55 | 5.405741e-52 |
| MsG0180004937.01 | MsG0380014013.01 | 0.830145 | 3.462698e-55 | 3.617650e-52 |
| MsG0180004987.01 | MsG0380014013.01 | 0.847631 | 1.023140e-59 | 1.829815e-56 |
| MsG0180005119.01 | MsG0380014013.01 | 0.847971 | 8.251459e-60 | 1.491621e-56 |
| MsG0180005150.01 | MsG0380014013.01 | 0.839864 | 1.227365e-57 | 1.717304e-54 |
| MsG0180005283.01 | MsG0380014013.01 | 0.801089 | 1.061444e-48 | 5.024943e-46 |
| MsG0180005568.01 | MsG0380014013.01 | 0.846362 | 2.277731e-59 | 3.910727e-56 |
| MsG0180005806.01 | MsG0380014013.01 | 0.817896 | 2.600188e-52 | 1.917462e-49 |
| MsG0180005879.01 | MsG0380014013.01 | 0.826816 | 2.203830e-54 | 2.089280e-51 |
| MsG0180006223.01 | MsG0380014013.01 | 0.819917 | 9.029644e-53 | 7.042336e-50 |
| MsG0280010926.01 | MsG0380014013.01 | 0.803826 | 2.896278e-49 | 1.470045e-46 |
| MsG0380013472.01 | MsG0380014013.01 | 0.819720 | 1.001669e-52 | 7.769085e-50 |
| MsG0380013889.01 | MsG0380014013.01 | 0.816301 | 5.935784e-52 | 4.190435e-49 |
| MsG0380013918.01 | MsG0380014013.01 | 0.806655 | 7.401616e-50 | 4.039512e-47 |
| MsG0380013919.01 | MsG0380014013.01 | 0.830950 | 2.200190e-55 | 2.353284e-52 |
| MsG0380013927.01 | MsG0380014013.01 | 0.940936 | 9.922850e-101 | 1.121128e-95 |
| MsG0380013930.01 | MsG0380014013.01 | 0.833130 | 6.364004e-56 | 7.262429e-53 |
| MsG0380013932.01 | MsG0380014013.01 | 0.833002 | 6.848840e-56 | 7.785521e-53 |
| MsG0380013933.01 | MsG0380014013.01 | 0.820570 | 6.395654e-53 | 5.078944e-50 |
| MsG0380013939.01 | MsG0380014013.01 | 0.847855 | 8.881293e-60 | 1.599657e-56 |
| MsG0380013955.01 | MsG0380014013.01 | 0.885071 | 1.114835e-71 | 7.543504e-68 |
| MsG0380013958.01 | MsG0380014013.01 | 0.894412 | 2.525892e-75 | 2.508137e-71 |
| MsG0380013964.01 | MsG0380014013.01 | 0.847464 | 1.137590e-59 | 2.023805e-56 |
| MsG0380013965.01 | MsG0380014013.01 | 0.855698 | 5.298054e-62 | 1.233844e-58 |
| MsG0380013970.01 | MsG0380014013.01 | 0.895270 | 1.124197e-75 | 1.158374e-71 |
| MsG0380013976.01 | MsG0380014013.01 | 0.808372 | 3.199418e-50 | 1.826237e-47 |
| MsG0380013978.01 | MsG0380014013.01 | 0.811785 | 5.884751e-51 | 3.676212e-48 |
| MsG0380013979.01 | MsG0380014013.01 | 0.833908 | 4.068743e-56 | 4.752864e-53 |
| MsG0380013980.01 | MsG0380014013.01 | 0.861386 | 1.064294e-63 | 3.006505e-60 |
| MsG0380013983.01 | MsG0380014013.01 | 0.800745 | 1.247602e-48 | 5.854575e-46 |
| MsG0380013992.01 | MsG0380014013.01 | 0.839057 | 1.989501e-57 | 2.715462e-54 |
| MsG0380013996.01 | MsG0380014013.01 | 0.869780 | 2.395837e-66 | 9.100008e-63 |
| MsG0380014000.01 | MsG0380014013.01 | 0.808077 | 3.697011e-50 | 2.093864e-47 |
| MsG0380014007.01 | MsG0380014013.01 | 0.869019 | 4.234774e-66 | 1.564883e-62 |
| MsG0380014009.01 | MsG0380014013.01 | 0.812453 | 4.208233e-51 | 2.676915e-48 |
| MsG0380014010.01 | MsG0380014013.01 | 0.812913 | 3.337786e-51 | 2.148854e-48 |
| MsG0380014012.01 | MsG0380014013.01 | 0.955528 | 2.462352e-113 | 7.499888e-108 |
| MsG0380014013.01 | MsG0380014014.01 | 0.813107 | 3.025423e-51 | 1.957860e-48 |
| MsG0380014013.01 | MsG0380014022.01 | 0.893568 | 5.567158e-75 | 5.331625e-71 |
| MsG0380014013.01 | MsG0380014030.01 | 0.820112 | 8.146564e-53 | 6.389072e-50 |
| MsG0380014013.01 | MsG0380014035.01 | 0.825165 | 5.439842e-54 | 4.920238e-51 |
| MsG0380014013.01 | MsG0380014036.01 | 0.817402 | 3.359553e-52 | 2.444236e-49 |
| MsG0380014013.01 | MsG0380014037.01 | 0.899919 | 1.230031e-77 | 1.553159e-73 |
| MsG0380014013.01 | MsG0380014038.01 | 0.859864 | 3.079069e-63 | 8.252394e-60 |
| MsG0380014013.01 | MsG0380014041.01 | 0.812657 | 3.797499e-51 | 2.428430e-48 |
| MsG0380014013.01 | MsG0380014042.01 | 0.817916 | 2.572746e-52 | 1.898325e-49 |
| MsG0380014013.01 | MsG0380014048.01 | 0.803885 | 2.815210e-49 | 1.431141e-46 |
| MsG0380014013.01 | MsG0380014049.01 | 0.832979 | 6.938289e-56 | 7.881695e-53 |
| MsG0380014013.01 | MsG0380014050.01 | 0.814202 | 1.737210e-51 | 1.158182e-48 |
| MsG0380014013.01 | MsG0380014052.01 | 0.843176 | 1.645904e-58 | 2.553645e-55 |
| MsG0380014013.01 | MsG0380014053.01 | 0.859080 | 5.298107e-63 | 1.381927e-59 |
| MsG0380014013.01 | MsG0380014055.01 | 0.815592 | 8.542057e-52 | 5.914400e-49 |
| MsG0380014013.01 | MsG0380014056.01 | 0.826353 | 2.842447e-54 | 2.659309e-51 |
| MsG0380014013.01 | MsG0380014729.01 | 0.842103 | 3.171793e-58 | 4.758995e-55 |
| MsG0380014013.01 | MsG0380016074.01 | 0.816795 | 4.599548e-52 | 3.291082e-49 |
| MsG0380014013.01 | MsG0380016452.01 | 0.823348 | 1.454022e-53 | 1.248687e-50 |
| MsG0380014013.01 | MsG0380016459.01 | 0.824138 | 9.492446e-54 | 8.336617e-51 |
| MsG0380014013.01 | MsG0380016623.01 | 0.802872 | 4.565562e-49 | 2.261315e-46 |
| MsG0380014013.01 | MsG0380016723.01 | 0.808588 | 2.877401e-50 | 1.651609e-47 |
| MsG0380014013.01 | MsG0380016751.01 | 0.828168 | 1.044426e-54 | 1.030009e-51 |
| MsG0380014013.01 | MsG0380016762.01 | 0.833903 | 4.081818e-56 | 4.767209e-53 |
| MsG0380014013.01 | MsG0380017006.01 | 0.820035 | 8.483323e-53 | 6.638278e-50 |
| MsG0380014013.01 | MsG0380017309.01 | 0.804446 | 2.152383e-49 | 1.109977e-46 |
| MsG0380014013.01 | MsG0380017976.01 | 0.827702 | 1.352097e-54 | 1.315327e-51 |
| MsG0380014013.01 | MsG0480018089.01 | 0.842525 | 2.451551e-58 | 3.727458e-55 |
| MsG0380014013.01 | MsG0480018297.01 | 0.816239 | 6.126362e-52 | 4.317565e-49 |
| MsG0380014013.01 | MsG0480018323.01 | 0.827551 | 1.469492e-54 | 1.423263e-51 |
| MsG0380014013.01 | MsG0480018324.01 | 0.822282 | 2.574386e-53 | 2.145306e-50 |
| MsG0380014013.01 | MsG0480018631.01 | 0.807816 | 4.201462e-50 | 2.363268e-47 |
| MsG0380014013.01 | MsG0480018844.01 | 0.826787 | 2.239324e-54 | 2.121103e-51 |
| MsG0380014013.01 | MsG0480018983.01 | 0.806608 | 7.572601e-50 | 4.128010e-47 |
| MsG0380014013.01 | MsG0480019592.01 | 0.808548 | 2.934075e-50 | 1.682478e-47 |
| MsG0380014013.01 | MsG0480019974.01 | 0.803110 | 4.076417e-49 | 2.031229e-46 |
| MsG0380014013.01 | MsG0480020049.01 | 0.835727 | 1.418179e-56 | 1.749321e-53 |
| MsG0380014013.01 | MsG0480020350.01 | 0.805663 | 1.197538e-49 | 6.372369e-47 |
| MsG0380014013.01 | MsG0480021303.01 | 0.836921 | 7.046024e-57 | 9.009161e-54 |
| MsG0380014013.01 | MsG0480021346.01 | 0.809765 | 1.609254e-50 | 9.531481e-48 |
| MsG0380014013.01 | MsG0480021367.01 | 0.810383 | 1.184723e-50 | 7.130617e-48 |
| MsG0380014013.01 | MsG0480021420.01 | 0.830710 | 2.519750e-55 | 2.676073e-52 |
| MsG0380014013.01 | MsG0480022300.01 | 0.824978 | 6.019980e-54 | 5.416103e-51 |
| MsG0380014013.01 | MsG0480022804.01 | 0.811822 | 5.776414e-51 | 3.612052e-48 |
| MsG0380014013.01 | MsG0480023166.01 | 0.804940 | 1.696680e-49 | 8.862933e-47 |
| MsG0380014013.01 | MsG0480023371.01 | 0.863493 | 2.392861e-64 | 7.267519e-61 |
| MsG0380014013.01 | MsG0480023422.01 | 0.833039 | 6.704660e-56 | 7.630095e-53 |
| MsG0380014013.01 | MsG0480023505.01 | 0.820302 | 7.370062e-53 | 5.809544e-50 |
| MsG0380014013.01 | MsG0480023881.01 | 0.823218 | 1.558757e-53 | 1.333813e-50 |
| MsG0380014013.01 | MsG0580024567.01 | 0.805539 | 1.271544e-49 | 6.744397e-47 |
| MsG0380014013.01 | MsG0580024982.01 | 0.829878 | 4.023090e-55 | 4.171152e-52 |
| MsG0380014013.01 | MsG0580025589.01 | 0.818664 | 1.742355e-52 | 1.312293e-49 |
| MsG0380014013.01 | MsG0580026430.01 | 0.838582 | 2.639185e-57 | 3.550694e-54 |
| MsG0380014013.01 | MsG0580027098.01 | 0.838478 | 2.807594e-57 | 3.765092e-54 |
| MsG0380014013.01 | MsG0580027254.01 | 0.839467 | 1.556916e-57 | 2.152023e-54 |
| MsG0380014013.01 | MsG0580028853.01 | 0.833752 | 4.451773e-56 | 5.175141e-53 |
| MsG0380014013.01 | MsG0580029918.01 | 0.809595 | 1.750742e-50 | 1.032265e-47 |
| MsG0380014013.01 | MsG0680030290.01 | 0.818605 | 1.796403e-52 | 1.350829e-49 |
| MsG0380014013.01 | MsG0680030776.01 | 0.802349 | 5.851666e-49 | 2.860068e-46 |
| MsG0380014013.01 | MsG0680032055.01 | 0.818272 | 2.137643e-52 | 1.592703e-49 |
| MsG0380014013.01 | MsG0680033224.01 | 0.854527 | 1.159659e-61 | 2.597631e-58 |
| MsG0380014013.01 | MsG0680033901.01 | 0.800997 | 1.108301e-48 | 5.234729e-46 |
| MsG0380014013.01 | MsG0680033902.01 | 0.808426 | 3.115348e-50 | 1.780766e-47 |
| MsG0380014013.01 | MsG0680033966.01 | 0.833547 | 5.007834e-56 | 5.786213e-53 |
| MsG0380014013.01 | MsG0680034724.01 | 0.802115 | 6.539695e-49 | 3.177104e-46 |
| MsG0380014013.01 | MsG0780037436.01 | 0.803145 | 4.007840e-49 | 1.998898e-46 |
| MsG0380014013.01 | MsG0780037705.01 | 0.945287 | 4.046051e-104 | 6.124732e-99 |
| MsG0380014013.01 | MsG0780037915.01 | 0.802105 | 6.570103e-49 | 3.191072e-46 |
| MsG0380014013.01 | MsG0780038496.01 | 0.810175 | 1.313566e-50 | 7.863312e-48 |
| MsG0380014013.01 | MsG0780038571.01 | 0.815694 | 8.109038e-52 | 5.630204e-49 |
| MsG0380014013.01 | MsG0780038653.01 | 0.809562 | 1.779929e-50 | 1.048518e-47 |
| MsG0380014013.01 | MsG0780038986.01 | 0.804419 | 2.179557e-49 | 1.123207e-46 |
| MsG0380014013.01 | MsG0780039596.01 | 0.806671 | 7.345015e-50 | 4.010312e-47 |
| MsG0380014013.01 | MsG0780039859.01 | 0.837226 | 5.890046e-57 | 7.600829e-54 |
| MsG0380014013.01 | MsG0780039975.01 | 0.811048 | 8.505752e-51 | 5.211442e-48 |
| MsG0380014013.01 | MsG0780040080.01 | 0.830006 | 3.744235e-55 | 3.895795e-52 |
| MsG0380014013.01 | MsG0780040314.01 | 0.899924 | 1.223677e-77 | 1.545602e-73 |
| MsG0380014013.01 | MsG0780040315.01 | 0.830244 | 3.275374e-55 | 3.431716e-52 |
| MsG0380014013.01 | MsG0780040317.01 | 0.838483 | 2.800229e-57 | 3.755697e-54 |
| MsG0380014013.01 | MsG0780040644.01 | 0.827551 | 1.469492e-54 | 1.423263e-51 |
| MsG0380014013.01 | MsG0780041289.01 | 0.807891 | 4.049677e-50 | 2.282278e-47 |
| MsG0380014013.01 | MsG0780041768.01 | 0.802808 | 4.706211e-49 | 2.327293e-46 |
| MsG0380014013.01 | MsG0780041792.01 | 0.834181 | 3.477777e-56 | 4.094885e-53 |
| MsG0380014013.01 | MsG0880042006.01 | 0.810154 | 1.327087e-50 | 7.940099e-48 |
| MsG0380014013.01 | MsG0880042921.01 | 0.834533 | 2.837372e-56 | 3.376985e-53 |
| MsG0380014013.01 | MsG0880043032.01 | 0.836117 | 1.129163e-56 | 1.409354e-53 |
| MsG0380014013.01 | MsG0880043075.01 | 0.804563 | 2.034348e-49 | 1.052283e-46 |
| MsG0380014013.01 | MsG0880043541.01 | 0.855013 | 8.384894e-62 | 1.908632e-58 |
| MsG0380014013.01 | MsG0880044312.01 | 0.814264 | 1.683097e-51 | 1.124039e-48 |
| MsG0380014013.01 | MsG0880044314.01 | 0.821235 | 4.496144e-53 | 3.637366e-50 |
| MsG0380014013.01 | MsG0880044440.01 | 0.839944 | 1.169836e-57 | 1.641096e-54 |
| MsG0380014013.01 | MsG0880044884.01 | 0.800721 | 1.261760e-48 | 5.917337e-46 |
| MsG0380014013.01 | MsG0880045073.01 | 0.815422 | 9.321491e-52 | 6.423860e-49 |
| MsG0380014013.01 | MsG0880045261.01 | 0.828041 | 1.120373e-54 | 1.100885e-51 |
| MsG0380014013.01 | MsG0880045283.01 | 0.876196 | 1.691955e-68 | 8.128829e-65 |
| MsG0380014013.01 | MsG0880045412.01 | 0.851295 | 9.743597e-61 | 1.963093e-57 |
| MsG0380014013.01 | MsG0880046288.01 | 0.803015 | 4.264070e-49 | 2.119562e-46 |
| MsG0280007026.01 | MsG0380014013.01 | 0.813390 | 2.622126e-51 | 1.710223e-48 |
| MsG0280007041.01 | MsG0380014013.01 | 0.888209 | 7.230391e-73 | 5.548628e-69 |
| MsG0280007042.01 | MsG0380014013.01 | 0.847904 | 8.606063e-60 | 1.552503e-56 |
| MsG0280007600.01 | MsG0380014013.01 | 0.801515 | 8.682182e-49 | 4.154404e-46 |
| MsG0280007654.01 | MsG0380014013.01 | 0.802658 | 5.053518e-49 | 2.489316e-46 |
| MsG0280008233.01 | MsG0380014013.01 | 0.816483 | 5.402119e-52 | 3.832464e-49 |
| MsG0280009751.01 | MsG0380014013.01 | 0.813295 | 2.751923e-51 | 1.790104e-48 |
| MsG0280010136.01 | MsG0380014013.01 | 0.801630 | 8.223603e-49 | 3.946271e-46 |
| MsG0280010198.01 | MsG0380014013.01 | 0.803938 | 2.744581e-49 | 1.397169e-46 |
| MsG0280010496.01 | MsG0380014013.01 | 0.822036 | 2.936256e-53 | 2.429458e-50 |
PPI
| Gene1 | Gene2 | Type |
|---|
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MsG0380014013.01.T01 | MTR_4g050880 | 98.361 | 61 | 1 | 0 | 1 | 61 | 1 | 61 | 1.04e-36 | 117 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MsG0380014013.01.T01 | ATCG00070 | 78.689 | 61 | 13 | 0 | 1 | 61 | 1 | 61 | 1.70e-28 | 96.7 |
Find 2 sgRNAs with CRISPR-Local
Find 10 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| TATGCTTAATATCTTTAGCT+TGG | 0.440922 | 3:+48649693 | None:intergenic |
| ACTGGCATAAAATCTACAAT+TGG | 0.453871 | 3:-48649796 | None:intergenic |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|---|---|---|---|---|
| !! | AGAAAAAACTACTTGAATAA+AGG | - | Chr3:48649741-48649760 | None:intergenic | 20.0% |
| !! | GAAAAAACTACTTGAATAAA+GGG | - | Chr3:48649740-48649759 | None:intergenic | 20.0% |
| !!! | TTATTCAAGTAGTTTTTTCT+TGG | + | Chr3:48649741-48649760 | MsG0380014013.01.T01:CDS | 20.0% |
| !!! | TTTTTCTCTTAGCTTTTGTT+TGG | + | Chr3:48649833-48649852 | MsG0380014013.01.T01:CDS | 25.0% |
| ACTGGCATAAAATCTACAAT+TGG | - | Chr3:48649799-48649818 | None:intergenic | 30.0% | |
| AGCTAAGAGAAAAAAGAGTA+AGG | - | Chr3:48649827-48649846 | None:intergenic | 30.0% | |
| GCTAAGAGAAAAAAGAGTAA+GGG | - | Chr3:48649826-48649845 | None:intergenic | 30.0% | |
| ! | AATTGGATTCAAAAAAGCGT+AGG | - | Chr3:48649782-48649801 | None:intergenic | 30.0% |
| !! | AAAAAGAGTAAGGGTATTAC+TGG | - | Chr3:48649817-48649836 | None:intergenic | 30.0% |
| ! | TTCAAAAAAGCGTAGGCTTC+AGG | - | Chr3:48649775-48649794 | None:intergenic | 40.0% |
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| Chr3 | gene | 48649694 | 48649879 | 48649694 | ID=MsG0380014013.01;Name=MsG0380014013.01 |
| Chr3 | mRNA | 48649694 | 48649879 | 48649694 | ID=MsG0380014013.01.T01;Parent=MsG0380014013.01;Name=MsG0380014013.01.T01;_AED=0.50;_eAED=0.50;_QI=0|-1|0|1|-1|0|1|0|61 |
| Chr3 | exon | 48649694 | 48649879 | 48649694 | ID=MsG0380014013.01.T01:exon:13747;Parent=MsG0380014013.01.T01 |
| Chr3 | CDS | 48649694 | 48649879 | 48649694 | ID=MsG0380014013.01.T01:cds;Parent=MsG0380014013.01.T01 |
| Gene Sequence |
| Protein sequence |
