AlfalfaGEDB | MsG0280010171.01

M. sativa cultivar ZhongmuNo.1 / MsG0280010171.01

KEGG: -
GO: GO:0003677 GO:0005634
PFAM: PF03110

Nr:

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Query id	Subject id	identity %	alignment length	mis matches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MsG0280010171.01.T01	XP_013464662.2	92.655	177	13	0	1	177	36	212	7.40E-104	307

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Swissprot:

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Query id	Subject id	identity %	alignment length	mis matches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MsG0280010171.01.T01	Q38741	60.177	113	41	1	30	142	22	130	8.26E-39	132

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Trembl:

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Query id	Subject id	identity %	alignment length	mis matches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MsG0280010171.01.T01	A0A072VA58	94.152	171	10	0	7	177	1	171	7.75e-101	297

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TFs/TRs:

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Gene ID	Type	Classification
MsG0280010171.01.T01	TF	SBP

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Protein Kinases:

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Gene ID	Type	Classification
No data available in table

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Network:

Co-expression Network

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Gene1	Gene2	correlation coefficient	p_value	FDR
No data available in table

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PPI

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Gene1	Gene2	Type
MsG0280010171.01	MsG0880044410.01	PPI
MsG0880044435.01	MsG0280010171.01	PPI

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Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MsG0280010171.01.T01	MTR_2g078770	94.152	171	10	0	7	177	1	171	1.97e-104	297
MsG0280010171.01.T01	MTR_8g463140	68.333	120	36	1	60	177	61	180	2.41e-51	162
MsG0280010171.01.T01	MTR_4g088555	66.667	93	31	0	47	139	46	138	3.77e-38	128
MsG0280010171.01.T01	MTR_2g014200	60.952	105	39	1	38	140	36	140	4.22e-38	128
MsG0280010171.01.T01	MTR_8g005960	61.765	102	39	0	37	138	160	261	6.27e-36	127
MsG0280010171.01.T01	MTR_3g085180	64.045	89	32	0	49	137	150	238	1.10e-35	129
MsG0280010171.01.T01	MTR_7g092930	67.470	83	27	0	61	143	65	147	4.93e-35	125
MsG0280010171.01.T01	MTR_2g046550	67.442	86	28	0	64	149	139	224	2.69e-34	127
MsG0280010171.01.T01	MTR_7g110320	67.391	92	30	0	56	147	137	228	2.74e-34	127
MsG0280010171.01.T01	MTR_7g110320	67.391	92	30	0	56	147	137	228	4.65e-34	127

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Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MsG0280010171.01.T01	AT1G53160	63.551	107	36	2	50	156	42	145	5.46e-39	131
MsG0280010171.01.T01	AT1G53160	63.551	107	36	2	50	156	42	145	9.70e-39	130
MsG0280010171.01.T01	AT1G53160	63.551	107	36	2	50	156	42	145	9.70e-39	130
MsG0280010171.01.T01	AT3G15270	53.741	147	63	2	7	148	1	147	1.75e-38	130
MsG0280010171.01.T01	AT1G02065	61.321	106	37	2	50	151	171	276	9.45e-37	130
MsG0280010171.01.T01	AT2G33810	71.250	80	23	0	60	139	50	129	6.82e-36	122
MsG0280010171.01.T01	AT1G69170	56.731	104	45	0	34	137	94	197	2.99e-35	127
MsG0280010171.01.T01	AT3G60030	62.136	103	34	2	64	166	127	224	1.19e-34	129
MsG0280010171.01.T01	AT1G20980	60.784	102	38	1	54	155	112	211	8.48e-34	126
MsG0280010171.01.T01	AT2G42200	68.675	83	26	0	62	144	72	154	1.87e-33	122

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Find 41 sgRNAs with CRISPR-Local

Find 224 sgRNAs with CRISPR-GE

CRISPR-Local

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CRISPR-Local
sgRNA_sequence	on_target_score	Position	Region
AAACATCAGTTGAGAAGGTT+TGG	0.391376	2:-65594981	MsG0280010171.01.T01:CDS
AACCACCACAACAGATGCTT+TGG	0.458273	2:+65594744	None:intergenic
AAGAAAGGTGTTTGTGTGAG+TGG	0.453403	2:-65594889	MsG0280010171.01.T01:CDS
AAGCAAGGAATACAATAGAG+GGG	0.716459	2:-65595008	MsG0280010171.01.T01:CDS
AATAGAAGTTGAAGAAGAGG+AGG	0.552525	2:-65594938	MsG0280010171.01.T01:CDS
AATGAACCTCACAAACCTTA+TGG	0.389353	2:+65594767	None:intergenic
ACTGCTGGAACTTGCACTGA+AGG	0.529046	2:-65591341	MsG0280010171.01.T01:CDS
ACTGGCCAGCAAAATCATGA+TGG	0.639807	2:-65591311	MsG0280010171.01.T01:CDS
AGAAAAGAGCCAAGTAGAGG+TGG	0.571945	2:-65594865	MsG0280010171.01.T01:CDS
AGAAAGGTGTTTGTGTGAGT+GGG	0.554222	2:-65594888	MsG0280010171.01.T01:CDS

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CRISPR-GE

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sgRNA_sequence	Strand	Position	Region	GC_content
AAAAATTCTCTCTCATCCAT+AGG	-	Chr2:65593715-65593734	MsG0280010171.01.T01:intron	30.0%
AAACAACTTGTGGTTTATGT+TGG	-	Chr2:65592471-65592490	MsG0280010171.01.T01:intron	30.0%
ACACCTTCATATAAATGAGT+GGG	-	Chr2:65592832-65592851	MsG0280010171.01.T01:intron	30.0%
ACTCATCAAGGTTTCAAATA+AGG	+	Chr2:65594713-65594732	None:intergenic	30.0%
AGAAATAGAAGTTGAAGAAG+AGG	-	Chr2:65591226-65591245	MsG0280010171.01.T01:exon	30.0%
AGGAGAAGAAATGATGAATT+TGG	-	Chr2:65592326-65592345	MsG0280010171.01.T01:intron	30.0%
AGTTAAAGGGAAAATGAAGA+AGG	-	Chr2:65592671-65592690	MsG0280010171.01.T01:intron	30.0%
AGTTTAGATTCTACGAAATG+CGG	+	Chr2:65591687-65591706	None:intergenic	30.0%
ATGAACATAACAAGTAGTTC+TGG	-	Chr2:65594898-65594917	MsG0280010171.01.T01:CDS	30.0%
CAACTTTCTACTTGACAAAA+AGG	+	Chr2:65591341-65591360	None:intergenic	30.0%

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Chromosome	Type	Strat	End	Strand	Name
Chr2	gene	65591155	65595034	65591155	ID=MsG0280010171.01;Name=MsG0280010171.01
Chr2	mRNA	65591155	65595034	65591155	ID=MsG0280010171.01.T01;Parent=MsG0280010171.01;Name=MsG0280010171.01.T01;_AED=0.47;_eAED=0.47;_QI=0\|1\|0.5\|1\|1\|1\|2\|89\|177
Chr2	exon	65591155	65591448	65591155	ID=MsG0280010171.01.T01:exon:5112;Parent=MsG0280010171.01.T01
Chr2	exon	65594706	65595034	65594706	ID=MsG0280010171.01.T01:exon:5111;Parent=MsG0280010171.01.T01
Chr2	CDS	65594706	65595034	65594706	ID=MsG0280010171.01.T01:cds;Parent=MsG0280010171.01.T01
Chr2	CDS	65591244	65591448	65591244	ID=MsG0280010171.01.T01:cds;Parent=MsG0280010171.01.T01
Chr2	three_prime_UTR	65591155	65591243	65591155	ID=MsG0280010171.01.T01:three_prime_utr;Parent=MsG0280010171.01.T01

Gene Sequence

>MsG0280010171.01.T01

ATGGAAGCAAGGAATACAATAGAGGGGAAGCAAACATCAGTTGAGAAGGTTTGGAAAGACGTCTTATTTGAAGAAATAGAAGTTGAAGAAGAGGAGGAAGGTGGTGTTGAAGATGATGAGAAGAAGAAAGGTGTTTGTGTGAGTGGGAGAAAAGAGCCAAGTAGAGGTGGAAGATTTTCTCCACCTTTTTGTCAAGTAGAAAGTTGTGGAGCTGATTTAACTTTTGCAAAGAGATATCATCGTCGCCATAAGGTTTGTGAGGTTCATTCCAAAGCATCTGTTGTGGTGGTTGCAGGGATAAGGCAGAGGTTTTGCCAACAATGTAGCAGGTTTCATGAGCTTGCAGAGTTTGATGAATCGAAAAGAAGCTGTCGAAGACGCTTAGCGCGACACAATGAACGGCGTCGTAAAAGCACTGCTGGAACTTGCACTGAAGGAAGCGGTACTGGCCAGCAAAATCATGATGGTGATTGGAGACAAATTCATATGAACATAACAAGTAGTTCTGGTCACGATTTTCTGAATTTCAGATGA

Protein sequence

>MsG0280010171.01.T01

MEARNTIEGKQTSVEKVWKDVLFEEIEVEEEEEGGVEDDEKKKGVCVSGRKEPSRGGRFSPPFCQVESCGADLTFAKRYHRRHKVCEVHSKASVVVVAGIRQRFCQQCSRFHELAEFDESKRSCRRRLARHNERRRKSTAGTCTEGSGTGQQNHDGDWRQIHMNITSSSGHDFLNFR*