AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar ZhongmuNo.1 / MsG0280010171.01

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MsG0280010171.01.T01 MTR_2g078770 94.152 171 10 0 7 177 1 171 1.97e-104 297
MsG0280010171.01.T01 MTR_8g463140 68.333 120 36 1 60 177 61 180 2.41e-51 162
MsG0280010171.01.T01 MTR_4g088555 66.667 93 31 0 47 139 46 138 3.77e-38 128
MsG0280010171.01.T01 MTR_2g014200 60.952 105 39 1 38 140 36 140 4.22e-38 128
MsG0280010171.01.T01 MTR_8g005960 61.765 102 39 0 37 138 160 261 6.27e-36 127
MsG0280010171.01.T01 MTR_3g085180 64.045 89 32 0 49 137 150 238 1.10e-35 129
MsG0280010171.01.T01 MTR_7g092930 67.470 83 27 0 61 143 65 147 4.93e-35 125
MsG0280010171.01.T01 MTR_2g046550 67.442 86 28 0 64 149 139 224 2.69e-34 127
MsG0280010171.01.T01 MTR_7g110320 67.391 92 30 0 56 147 137 228 2.74e-34 127
MsG0280010171.01.T01 MTR_7g110320 67.391 92 30 0 56 147 137 228 4.65e-34 127
MsG0280010171.01.T01 MTR_1g086250 62.376 101 38 0 49 149 132 232 5.69e-34 127
MsG0280010171.01.T01 MTR_7g444860 63.158 95 35 0 56 150 141 235 1.41e-33 121
MsG0280010171.01.T01 MTR_1g053715 69.136 81 25 0 57 137 67 147 1.63e-33 122
MsG0280010171.01.T01 MTR_2g461920 59.813 107 42 1 63 169 170 275 1.91e-33 124
MsG0280010171.01.T01 MTR_4g109770 70.667 75 22 0 63 137 122 196 4.97e-32 119
MsG0280010171.01.T01 MTR_5g046670 58.947 95 39 0 62 156 242 336 6.67e-32 119
MsG0280010171.01.T01 MTR_1g035010 70.130 77 23 0 62 138 102 178 4.74e-31 118
MsG0280010171.01.T01 MTR_8g080690 67.105 76 25 0 62 137 149 224 1.85e-30 114
MsG0280010171.01.T01 MTR_3g099080 54.839 93 42 0 64 156 98 190 3.86e-30 113
MsG0280010171.01.T01 MTR_8g080670 63.158 76 28 0 61 136 159 234 9.15e-30 112
MsG0280010171.01.T01 MTR_7g028740 51.485 101 48 1 63 163 76 175 5.49e-29 110
MsG0280010171.01.T01 MTR_8g080680 42.424 132 74 2 7 137 75 205 9.04e-29 110
MsG0280010171.01.T01 MTR_8g096780 60.714 84 33 0 64 147 99 182 1.37e-28 109
MsG0280010171.01.T01 MTR_2g020620 56.522 69 28 1 71 137 95 163 6.59e-20 86.7
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MsG0280010171.01.T01 AT1G53160 63.551 107 36 2 50 156 42 145 5.46e-39 131
MsG0280010171.01.T01 AT1G53160 63.551 107 36 2 50 156 42 145 9.70e-39 130
MsG0280010171.01.T01 AT1G53160 63.551 107 36 2 50 156 42 145 9.70e-39 130
MsG0280010171.01.T01 AT3G15270 53.741 147 63 2 7 148 1 147 1.75e-38 130
MsG0280010171.01.T01 AT1G02065 61.321 106 37 2 50 151 171 276 9.45e-37 130
MsG0280010171.01.T01 AT2G33810 71.250 80 23 0 60 139 50 129 6.82e-36 122
MsG0280010171.01.T01 AT1G69170 56.731 104 45 0 34 137 94 197 2.99e-35 127
MsG0280010171.01.T01 AT3G60030 62.136 103 34 2 64 166 127 224 1.19e-34 129
MsG0280010171.01.T01 AT1G20980 60.784 102 38 1 54 155 112 211 8.48e-34 126
MsG0280010171.01.T01 AT2G42200 68.675 83 26 0 62 144 72 154 1.87e-33 122
MsG0280010171.01.T01 AT2G47070 55.963 109 46 1 64 172 106 212 8.59e-33 123
MsG0280010171.01.T01 AT5G43270 61.798 89 34 0 58 146 163 251 4.04e-32 119
MsG0280010171.01.T01 AT5G43270 61.798 89 34 0 58 146 163 251 4.04e-32 119
MsG0280010171.01.T01 AT5G43270 61.798 89 34 0 58 146 163 251 4.04e-32 119
MsG0280010171.01.T01 AT1G76580 54.717 106 47 1 62 167 112 216 1.04e-31 120
MsG0280010171.01.T01 AT1G76580 54.717 106 47 1 62 167 112 216 2.23e-31 119
MsG0280010171.01.T01 AT1G76580 54.717 106 47 1 62 167 112 216 2.26e-31 119
MsG0280010171.01.T01 AT3G57920 67.089 79 26 0 64 142 59 137 5.46e-31 115
MsG0280010171.01.T01 AT1G27370 57.143 91 39 0 47 137 159 249 4.01e-30 112
MsG0280010171.01.T01 AT1G27370 57.143 91 39 0 47 137 159 249 1.12e-29 112
MsG0280010171.01.T01 AT1G27370 57.143 91 39 0 47 137 159 249 1.12e-29 112
MsG0280010171.01.T01 AT1G27370 57.143 91 39 0 47 137 159 249 1.12e-29 112
MsG0280010171.01.T01 AT1G27370 57.143 91 39 0 47 137 159 249 1.12e-29 112
MsG0280010171.01.T01 AT1G27370 57.143 91 39 0 47 137 159 249 1.12e-29 112
MsG0280010171.01.T01 AT1G27370 57.143 91 39 0 47 137 159 249 1.12e-29 112
MsG0280010171.01.T01 AT5G50670 63.158 76 28 0 62 137 99 174 7.93e-29 109
MsG0280010171.01.T01 AT5G50670 63.158 76 28 0 62 137 99 174 7.93e-29 109
MsG0280010171.01.T01 AT5G50570 63.158 76 28 0 62 137 99 174 7.93e-29 109
MsG0280010171.01.T01 AT5G50570 63.158 76 28 0 62 137 99 174 7.93e-29 109
MsG0280010171.01.T01 AT1G27360 60.976 82 32 0 56 137 167 248 9.00e-29 110
MsG0280010171.01.T01 AT1G27360 60.976 82 32 0 56 137 167 248 9.00e-29 110
MsG0280010171.01.T01 AT1G27360 60.976 82 32 0 56 137 167 248 9.00e-29 110
MsG0280010171.01.T01 AT1G27360 60.976 82 32 0 56 137 167 248 9.00e-29 110
MsG0280010171.01.T01 AT5G18830 47.368 95 47 1 64 155 138 232 6.90e-22 92.4
MsG0280010171.01.T01 AT5G18830 47.368 95 47 1 64 155 138 232 7.00e-22 92.4
MsG0280010171.01.T01 AT5G18830 47.368 95 47 1 64 155 138 232 7.59e-22 92.0
MsG0280010171.01.T01 AT1G02065 60.811 74 24 2 50 118 171 244 1.39e-21 88.6
MsG0280010171.01.T01 AT2G47070 74.510 51 13 0 88 138 1 51 6.98e-20 86.7

Find 41 sgRNAs with CRISPR-Local

Find 224 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
TGCTTTCTCTCTTCTGTCAT+TGG 0.288213 2:-65591200 MsG0280010171.01.T01:three_prime_UTR
CAACTTTCTACTTGACAAAA+AGG 0.293853 2:+65594829 None:intergenic
GCATCTGTTGTGGTGGTTGC+AGG 0.368915 2:-65594739 MsG0280010171.01.T01:CDS
AATGAACCTCACAAACCTTA+TGG 0.389353 2:+65594767 None:intergenic
AAACATCAGTTGAGAAGGTT+TGG 0.391376 2:-65594981 MsG0280010171.01.T01:CDS
GATCACACCTGCTACATTGT+TGG 0.396569 2:+65594698 None:intergenic
ATGAACATAACAAGTAGTTC+TGG 0.412406 2:-65591269 MsG0280010171.01.T01:CDS
AGCAAAATCATGATGGTGAT+TGG 0.426111 2:-65591304 MsG0280010171.01.T01:CDS
TATATTCTCGTAACATAGAA+AGG 0.434000 2:+65591146 None:intergenic
TGCACTGAAGGAAGCGGTAC+TGG 0.443322 2:-65591329 MsG0280010171.01.T01:CDS
AAGAAAGGTGTTTGTGTGAG+TGG 0.453403 2:-65594889 MsG0280010171.01.T01:CDS
AACCACCACAACAGATGCTT+TGG 0.458273 2:+65594744 None:intergenic
TTGTGGTGGTTGCAGGGATA+AGG 0.463071 2:-65594732 MsG0280010171.01.T01:CDS
CGGCGTCGTAAAAGCACTGC+TGG 0.466987 2:-65591356 MsG0280010171.01.T01:CDS
AGAAATAGAAGTTGAAGAAG+AGG 0.475722 2:-65594941 MsG0280010171.01.T01:CDS
GAAAATCTTCCACCTCTACT+TGG 0.489950 2:+65594856 None:intergenic
GAGATATCATCGTCGCCATA+AGG 0.496552 2:-65594782 MsG0280010171.01.T01:CDS
GGAAGCAAGGAATACAATAG+AGG 0.502654 2:-65595010 MsG0280010171.01.T01:CDS
GAAGCAAGGAATACAATAGA+GGG 0.522960 2:-65595009 MsG0280010171.01.T01:CDS
GTTGAAGAAGAGGAGGAAGG+TGG 0.527678 2:-65594931 MsG0280010171.01.T01:CDS
ACTGCTGGAACTTGCACTGA+AGG 0.529046 2:-65591341 MsG0280010171.01.T01:CDS
CATTGGTTCCTTACATGCCA+AGG 0.533613 2:-65591183 MsG0280010171.01.T01:three_prime_UTR
GAAGTTGAAGAAGAGGAGGA+AGG 0.550030 2:-65594934 MsG0280010171.01.T01:CDS
ATATTCTCGTAACATAGAAA+GGG 0.552452 2:+65591147 None:intergenic
AATAGAAGTTGAAGAAGAGG+AGG 0.552525 2:-65594938 MsG0280010171.01.T01:CDS
AGAAAGGTGTTTGTGTGAGT+GGG 0.554222 2:-65594888 MsG0280010171.01.T01:CDS
TTCCAAAGCATCTGTTGTGG+TGG 0.554775 2:-65594746 MsG0280010171.01.T01:CDS
GACAGAAGAGAGAAAGCATA+CGG 0.557349 2:+65591205 None:intergenic
GAAGATGATGAGAAGAAGAA+AGG 0.566806 2:-65594904 MsG0280010171.01.T01:CDS
GAAGCAAACATCAGTTGAGA+AGG 0.569996 2:-65594986 MsG0280010171.01.T01:CDS
AGAAAAGAGCCAAGTAGAGG+TGG 0.571945 2:-65594865 MsG0280010171.01.T01:CDS
TGGTTGCAGGGATAAGGCAG+AGG 0.586383 2:-65594726 MsG0280010171.01.T01:CDS
GGAACTTGCACTGAAGGAAG+CGG 0.610870 2:-65591335 MsG0280010171.01.T01:CDS
CATCTGTTGTGGTGGTTGCA+GGG 0.612833 2:-65594738 MsG0280010171.01.T01:CDS
CATAGTACACAATGGAAGCA+AGG 0.613876 2:-65595023 None:intergenic
TCATTCCAAAGCATCTGTTG+TGG 0.621345 2:-65594749 MsG0280010171.01.T01:CDS
GGGAGAAAAGAGCCAAGTAG+AGG 0.628005 2:-65594868 MsG0280010171.01.T01:CDS
ACTGGCCAGCAAAATCATGA+TGG 0.639807 2:-65591311 MsG0280010171.01.T01:CDS
TCGTCGCCATAAGGTTTGTG+AGG 0.640730 2:-65594773 MsG0280010171.01.T01:CDS
GCTTAGCGCGACACAATGAA+CGG 0.657384 2:-65591376 MsG0280010171.01.T01:CDS
AAGCAAGGAATACAATAGAG+GGG 0.716459 2:-65595008 MsG0280010171.01.T01:CDS

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!! ATATTATATATATATATATA+GGG + Chr2:65592914-65592895 None:intergenic 0.0%
!! TATATTATATATATATATAT+AGG + Chr2:65592913-65592894 None:intergenic 0.0%
!! AACCATAATATATATATATA+TGG - Chr2:65591981-65592000 MsG0280010171.01.T01:intron 10.0%
!! ACCATAATATATATATATAT+GGG - Chr2:65591982-65592001 MsG0280010171.01.T01:intron 10.0%
!! TAAAAAAAAAGTAACATAAA+TGG + Chr2:65592132-65592151 None:intergenic 10.0%
!! TTCAATATAATTATATTTGT+CGG + Chr2:65591596-65591615 None:intergenic 10.0%
!!! AAAAAACTTTTTTTATTATG+TGG + Chr2:65593974-65593993 None:intergenic 10.0%
!!! ACTTATTATTTTTATAGTTT+AGG - Chr2:65593093-65593112 MsG0280010171.01.T01:intron 10.0%
!!! ATTTCAAATATTTTTTTTAC+AGG + Chr2:65593826-65593845 None:intergenic 10.0%
!!! CTTATTATTTTTATAGTTTA+GGG - Chr2:65593094-65593113 MsG0280010171.01.T01:intron 10.0%
!!! TAAAAAAATTTGTTTGAAAA+AGG + Chr2:65594468-65594487 None:intergenic 10.0%
!!! TATGTTACTTTTTTTTTAAT+CGG - Chr2:65592134-65592153 MsG0280010171.01.T01:intron 10.0%
!!! TTCAAATTTTTATTTTTACA+GGG - Chr2:65594499-65594518 MsG0280010171.01.T01:intron 10.0%
!!! TTTCAAATATTTTTTTTACA+GGG + Chr2:65593825-65593844 None:intergenic 10.0%
!!! TTTCAAATTTTTATTTTTAC+AGG - Chr2:65594498-65594517 MsG0280010171.01.T01:intron 10.0%
!! AAATATTAAAAGATTGAATG+AGG - Chr2:65592581-65592600 MsG0280010171.01.T01:intron 15.0%
!! ATATGATCGAAAAAATTATT+TGG + Chr2:65594072-65594091 None:intergenic 15.0%
!! TCCCATATATATATATATTA+TGG + Chr2:65591986-65592005 None:intergenic 15.0%
!! TTAAAATAAGTATATGTGAA+GGG + Chr2:65592760-65592779 None:intergenic 15.0%
!!! AAAAATCTGTTTTATTAGTT+GGG - Chr2:65593381-65593400 MsG0280010171.01.T01:intron 15.0%
!!! AGATTTTTTTTTTAATTACG+TGG - Chr2:65594380-65594399 MsG0280010171.01.T01:intron 15.0%
!!! CAAATTTAAGTTTTAAAACA+TGG + Chr2:65591794-65591813 None:intergenic 15.0%
!!! TCACATATACTTATTTTAAA+AGG - Chr2:65592761-65592780 MsG0280010171.01.T01:intron 15.0%
!!! TTCAAACAAATTTTTTTACA+GGG - Chr2:65594470-65594489 MsG0280010171.01.T01:intron 15.0%
!!! TTTAAAATAAGTATATGTGA+AGG + Chr2:65592761-65592780 None:intergenic 15.0%
!!! TTTCAAACAAATTTTTTTAC+AGG - Chr2:65594469-65594488 MsG0280010171.01.T01:intron 15.0%
!! CCATAAACTTTGATTATAAT+AGG - Chr2:65592971-65592990 MsG0280010171.01.T01:intron 20.0%
!! CCTATTATAATCAAAGTTTA+TGG + Chr2:65592974-65592993 None:intergenic 20.0%
!! GTCTTTAACATTTAATAGTA+TGG - Chr2:65593036-65593055 MsG0280010171.01.T01:intron 20.0%
!! TAAAAACAAGTATATGTGAA+GGG - Chr2:65592091-65592110 MsG0280010171.01.T01:intron 20.0%
!! TTAAAAACAAGTATATGTGA+AGG - Chr2:65592090-65592109 MsG0280010171.01.T01:intron 20.0%
!!! AAGTTTTCGAAGTTTTTTTT+CGG - Chr2:65594322-65594341 MsG0280010171.01.T01:intron 20.0%
!!! ACCATTATAAGTATATTTTC+GGG + Chr2:65593498-65593517 None:intergenic 20.0%
!!! ACTCTTTCTTTTATTTGAAT+AGG - Chr2:65591704-65591723 MsG0280010171.01.T01:intron 20.0%
!!! CAAAAATCTGTTTTATTAGT+TGG - Chr2:65593380-65593399 MsG0280010171.01.T01:intron 20.0%
!!! CACCATTATAAGTATATTTT+CGG + Chr2:65593499-65593518 None:intergenic 20.0%
!!! CTTATTTTAAAAGGAGAAAT+TGG - Chr2:65592770-65592789 MsG0280010171.01.T01:intron 20.0%
!!! GAATATGCTCTTTTTATATT+GGG - Chr2:65593174-65593193 MsG0280010171.01.T01:intron 20.0%
!!! TGAATATGCTCTTTTTATAT+TGG - Chr2:65593173-65593192 MsG0280010171.01.T01:intron 20.0%
!!! TTTCACTTTTAATTTGAATG+AGG - Chr2:65592306-65592325 MsG0280010171.01.T01:intron 20.0%
! AAAAAAAAAACACACAAGCA+AGG + Chr2:65591909-65591928 None:intergenic 25.0%
! AAAACTAACCCAAATTACAA+AGG - Chr2:65594531-65594550 MsG0280010171.01.T01:intron 25.0%
! AAAATCAAGTTTACCATCTA+TGG + Chr2:65591762-65591781 None:intergenic 25.0%
! ACTGTTAAAATCTTTGTCAT+CGG - Chr2:65592243-65592262 MsG0280010171.01.T01:intron 25.0%
! AGATATTCTAAATTGAGTCA+AGG - Chr2:65593604-65593623 MsG0280010171.01.T01:intron 25.0%
! CATGCTACTATTTATATAGA+TGG + Chr2:65591862-65591881 None:intergenic 25.0%
! CGAAAATATACTTATAATGG+TGG - Chr2:65593497-65593516 MsG0280010171.01.T01:intron 25.0%
! GATATTCTAAATTGAGTCAA+GGG - Chr2:65593605-65593624 MsG0280010171.01.T01:intron 25.0%
! TCCCGAAAATATACTTATAA+TGG - Chr2:65593494-65593513 MsG0280010171.01.T01:intron 25.0%
!! ATTTTCGTCCTTTGTAATTT+GGG + Chr2:65594542-65594561 None:intergenic 25.0%
!! TTTTAATACCATGAAGATGA+AGG + Chr2:65593277-65593296 None:intergenic 25.0%
!!! AAAGTAGCAACAAGTTTTAA+AGG - Chr2:65592516-65592535 MsG0280010171.01.T01:intron 25.0%
!!! AAGTAGCAACAAGTTTTAAA+GGG - Chr2:65592517-65592536 MsG0280010171.01.T01:intron 25.0%
!!! ATCTCTAAAACTTAGAGTTT+TGG + Chr2:65593564-65593583 None:intergenic 25.0%
!!! ATGGAACATTTCTTTGTTTT+GGG + Chr2:65594683-65594702 None:intergenic 25.0%
!!! CGTCTTTAGTTTTACTTTAA+AGG + Chr2:65594575-65594594 None:intergenic 25.0%
!!! CTTTTTTGTCCAAATATAGA+AGG - Chr2:65592793-65592812 MsG0280010171.01.T01:intron 25.0%
!!! TTGACCTTTTTCACTATATT+AGG - Chr2:65594655-65594674 MsG0280010171.01.T01:intron 25.0%
AAAAATTCTCTCTCATCCAT+AGG - Chr2:65593715-65593734 MsG0280010171.01.T01:intron 30.0%
AAACAACTTGTGGTTTATGT+TGG - Chr2:65592471-65592490 MsG0280010171.01.T01:intron 30.0%
ACACCTTCATATAAATGAGT+GGG - Chr2:65592832-65592851 MsG0280010171.01.T01:intron 30.0%
ACTCATCAAGGTTTCAAATA+AGG + Chr2:65594713-65594732 None:intergenic 30.0%
AGAAATAGAAGTTGAAGAAG+AGG - Chr2:65591226-65591245 MsG0280010171.01.T01:exon 30.0%
AGGAGAAGAAATGATGAATT+TGG - Chr2:65592326-65592345 MsG0280010171.01.T01:intron 30.0%
AGTTAAAGGGAAAATGAAGA+AGG - Chr2:65592671-65592690 MsG0280010171.01.T01:intron 30.0%
AGTTTAGATTCTACGAAATG+CGG + Chr2:65591687-65591706 None:intergenic 30.0%
ATGAACATAACAAGTAGTTC+TGG - Chr2:65594898-65594917 MsG0280010171.01.T01:CDS 30.0%
CAACTTTCTACTTGACAAAA+AGG + Chr2:65591341-65591360 None:intergenic 30.0%
CATTGTACACCTTCTATATT+TGG + Chr2:65592805-65592824 None:intergenic 30.0%
GAAAATGAAGAAGGAATATG+GGG - Chr2:65592680-65592699 MsG0280010171.01.T01:intron 30.0%
GGAAAATGAAGAAGGAATAT+GGG - Chr2:65592679-65592698 MsG0280010171.01.T01:intron 30.0%
TAAAGGGGTAGAAATAACAA+AGG - Chr2:65592533-65592552 MsG0280010171.01.T01:intron 30.0%
TCATCTAAACTTATGAAGCA+CGG - Chr2:65591508-65591527 MsG0280010171.01.T01:intron 30.0%
TTCATAAGTTTAGATGACAG+TGG + Chr2:65591505-65591524 None:intergenic 30.0%
TTTCAAATAAGGTGAATGCA+TGG + Chr2:65594702-65594721 None:intergenic 30.0%
TTTGGAAAACCTTTCTTTGT+TGG + Chr2:65593238-65593257 None:intergenic 30.0%
! AATTTGTTTGAAAAAGGTCC+TGG + Chr2:65594462-65594481 None:intergenic 30.0%
! AGGGTCTTTATTATTTTCCT+TGG + Chr2:65595004-65595023 None:intergenic 30.0%
! ATCCAAGAATTTTGAATAGG+GGG - Chr2:65591834-65591853 MsG0280010171.01.T01:intron 30.0%
! ATTATTTTCCTTGGCATGTA+AGG + Chr2:65594995-65595014 None:intergenic 30.0%
! GATCCAAGAATTTTGAATAG+GGG - Chr2:65591833-65591852 MsG0280010171.01.T01:intron 30.0%
! GATTTTCGTCCTTTGTAATT+TGG + Chr2:65594543-65594562 None:intergenic 30.0%
! GGATCCAAGAATTTTGAATA+GGG - Chr2:65591832-65591851 MsG0280010171.01.T01:intron 30.0%
! TTTTGTCAAGTAGAAAGTTG+TGG - Chr2:65591341-65591360 MsG0280010171.01.T01:CDS 30.0%
!! CATGGAACATTTCTTTGTTT+TGG + Chr2:65594684-65594703 None:intergenic 30.0%
!! CTAAAACTTAGAGTTTTGGT+TGG + Chr2:65593560-65593579 None:intergenic 30.0%
!!! AGTAGCAACAAGTTTTAAAG+GGG - Chr2:65592518-65592537 MsG0280010171.01.T01:intron 30.0%
!!! TGGAACATTTCTTTGTTTTG+GGG + Chr2:65594682-65594701 None:intergenic 30.0%
!!! TTGCTACTTTTGAGATATAC+TGG + Chr2:65592506-65592525 None:intergenic 30.0%
AAAATTCTTGGATCCGTTAG+TGG + Chr2:65591827-65591846 None:intergenic 35.0%
AAACATCAGTTGAGAAGGTT+TGG - Chr2:65591186-65591205 MsG0280010171.01.T01:three_prime_UTR 35.0%
AAAGATTGAATGAGGTTGTG+AGG - Chr2:65592589-65592608 MsG0280010171.01.T01:intron 35.0%
AAATCATCACAAAAGTGGCT+AGG + Chr2:65593883-65593902 None:intergenic 35.0%
AAGCAAGGAATACAATAGAG+GGG - Chr2:65591159-65591178 MsG0280010171.01.T01:three_prime_UTR 35.0%
AATAGAAGTTGAAGAAGAGG+AGG - Chr2:65591229-65591248 MsG0280010171.01.T01:exon 35.0%
AATCATCACAAAAGTGGCTA+GGG + Chr2:65593882-65593901 None:intergenic 35.0%
AATGAACCTCACAAACCTTA+TGG + Chr2:65591403-65591422 None:intergenic 35.0%
ACACCCACTCATTTATATGA+AGG - Chr2:65592016-65592035 MsG0280010171.01.T01:intron 35.0%
ACACCCACTCATTTATATGA+AGG + Chr2:65592035-65592016 None:intergenic 35.0%
ACCACCTTCATATAAATGAG+TGG + Chr2:65592023-65592042 None:intergenic 35.0%
AGACATATCATCTCAACTCT+TGG + Chr2:65593473-65593492 None:intergenic 35.0%
AGAGGTAGAAAACAAACACA+AGG + Chr2:65592417-65592436 None:intergenic 35.0%
ATAAGTTTAGATGACAGTGG+AGG + Chr2:65591502-65591521 None:intergenic 35.0%
ATGCCAAATCATCACAAAAG+TGG + Chr2:65593888-65593907 None:intergenic 35.0%
CAAAACTGGACCAAATTACA+GGG + Chr2:65593794-65593813 None:intergenic 35.0%
CACACCTTCATATAAATGAG+TGG - Chr2:65592831-65592850 MsG0280010171.01.T01:intron 35.0%
CATCTATGGTATTCTACTAG+CGG + Chr2:65591748-65591767 None:intergenic 35.0%
CATTGTACACCTTCTATGTT+TGG - Chr2:65592048-65592067 MsG0280010171.01.T01:intron 35.0%
CCACCTTCATATAAATGAGT+GGG + Chr2:65592022-65592041 None:intergenic 35.0%
CGTTACAAAATCAACTTGCA+AGG + Chr2:65593532-65593551 None:intergenic 35.0%
CTTTCTACTTGACAAAAAGG+TGG + Chr2:65591338-65591357 None:intergenic 35.0%
GAAGATGATGAGAAGAAGAA+AGG - Chr2:65591263-65591282 MsG0280010171.01.T01:CDS 35.0%
GAAGCAAGGAATACAATAGA+GGG - Chr2:65591158-65591177 MsG0280010171.01.T01:three_prime_UTR 35.0%
GAGGTAGAAAACAAACACAA+GGG + Chr2:65592416-65592435 None:intergenic 35.0%
GGGAAAATGAAGAAGGAATA+TGG - Chr2:65592678-65592697 MsG0280010171.01.T01:intron 35.0%
TAATCACTTCTCTGTTTGAG+TGG - Chr2:65592445-65592464 MsG0280010171.01.T01:intron 35.0%
TCAAAACTGGACCAAATTAC+AGG + Chr2:65593795-65593814 None:intergenic 35.0%
TGAGTGGAAAAAACAACTTG+TGG - Chr2:65592461-65592480 MsG0280010171.01.T01:intron 35.0%
TGATAATCGTCCCTGTAATT+TGG - Chr2:65593781-65593800 MsG0280010171.01.T01:intron 35.0%
TGCTGAATAATCCTTACCTA+TGG + Chr2:65593734-65593753 None:intergenic 35.0%
TTAGTTGAGCCAACAAAGAA+AGG - Chr2:65593226-65593245 MsG0280010171.01.T01:intron 35.0%
TTGAATCTTGTTGCCACTAA+CGG - Chr2:65591811-65591830 MsG0280010171.01.T01:intron 35.0%
! AATCACCATCATGATTTTGC+TGG + Chr2:65594864-65594883 None:intergenic 35.0%
! AGCAAAATCATGATGGTGAT+TGG - Chr2:65594863-65594882 MsG0280010171.01.T01:CDS 35.0%
! CGGATCCAAGAATTTTGAAT+AGG - Chr2:65591831-65591850 MsG0280010171.01.T01:intron 35.0%
! GCTTTTTGTCCAAACATAGA+AGG + Chr2:65592060-65592079 None:intergenic 35.0%
! TTGGGACTTTGGAAGTTAAA+GGG - Chr2:65592658-65592677 MsG0280010171.01.T01:intron 35.0%
!! AACTTGACTCGACTTTTCAT+TGG + Chr2:65593346-65593365 None:intergenic 35.0%
!!! AGTCGAGTCAAGTTAGTTTT+AGG - Chr2:65593352-65593371 MsG0280010171.01.T01:intron 35.0%
!!! GGAACATTTCTTTGTTTTGG+GGG + Chr2:65594681-65594700 None:intergenic 35.0%
!!! TAGCCACTTTTGTGATGATT+TGG - Chr2:65593882-65593901 MsG0280010171.01.T01:intron 35.0%
AATTGGAATCGCAAGAGAGA+AGG - Chr2:65592634-65592653 MsG0280010171.01.T01:intron 40.0%
ACAGGGACGAAAATCAAAAC+TGG + Chr2:65594535-65594516 None:intergenic 40.0%
ATGCCAAATCAGATCAGAAG+TGG - Chr2:65594434-65594453 MsG0280010171.01.T01:intron 40.0%
ATTGGAATCGCAAGAGAGAA+GGG - Chr2:65592635-65592654 MsG0280010171.01.T01:intron 40.0%
CAGTCTGATAAGCTTCCAAA+CGG + Chr2:65592227-65592246 None:intergenic 40.0%
CCCACTCATTTATATGAAGG+TGG - Chr2:65592019-65592038 MsG0280010171.01.T01:intron 40.0%
CGCTAGTAGAATACCATAGA+TGG - Chr2:65591746-65591765 MsG0280010171.01.T01:intron 40.0%
GAAAATCTTCCACCTCTACT+TGG + Chr2:65591314-65591333 None:intergenic 40.0%
GAAGCAAACATCAGTTGAGA+AGG - Chr2:65591181-65591200 MsG0280010171.01.T01:three_prime_UTR 40.0%
GAAGTCACTCATGTCACTAT+GGG - Chr2:65593403-65593422 MsG0280010171.01.T01:intron 40.0%
GATTCTACGAAATGCGGATA+CGG + Chr2:65591681-65591700 None:intergenic 40.0%
GGAAGCAAGGAATACAATAG+AGG - Chr2:65591157-65591176 MsG0280010171.01.T01:three_prime_UTR 40.0%
GGGGCCTAATATAGTGAAAA+AGG + Chr2:65594662-65594681 None:intergenic 40.0%
TGCCAAATCAGATCAGAAGT+GGG - Chr2:65594435-65594454 MsG0280010171.01.T01:intron 40.0%
TGCCCCCTATTCAAAATTCT+TGG + Chr2:65591839-65591858 None:intergenic 40.0%
TGCTTTCTCTCTTCTGTCAT+TGG - Chr2:65594967-65594986 MsG0280010171.01.T01:CDS 40.0%
TTCTCTCTCATCCATAGGTA+AGG - Chr2:65593720-65593739 MsG0280010171.01.T01:intron 40.0%
! GACAGAAGAGAGAAAGCATA+CGG + Chr2:65594965-65594984 None:intergenic 40.0%
! GTTGGGACTTTGGAAGTTAA+AGG - Chr2:65592657-65592676 MsG0280010171.01.T01:intron 40.0%
! TCATTCCAAAGCATCTGTTG+TGG - Chr2:65591418-65591437 MsG0280010171.01.T01:CDS 40.0%
! TCGACTTTTCATTGGTGTGA+AGG + Chr2:65593338-65593357 None:intergenic 40.0%
!! AAGAAAGGTGTTTGTGTGAG+TGG - Chr2:65591278-65591297 MsG0280010171.01.T01:CDS 40.0%
!! ACTTCTGATCTGATTTGGCA+TGG + Chr2:65594435-65594454 None:intergenic 40.0%
!! AGAAAGGTGTTTGTGTGAGT+GGG - Chr2:65591279-65591298 MsG0280010171.01.T01:CDS 40.0%
AACCACCACAACAGATGCTT+TGG + Chr2:65591426-65591445 None:intergenic 45.0%
AACGACACATGTCTGACATC+AGG + Chr2:65591556-65591575 None:intergenic 45.0%
AATCGCAAGAGAGAAGGGTT+GGG - Chr2:65592640-65592659 MsG0280010171.01.T01:intron 45.0%
ACTGGCCAGCAAAATCATGA+TGG - Chr2:65594856-65594875 MsG0280010171.01.T01:CDS 45.0%
AGAAAAGAGCCAAGTAGAGG+TGG - Chr2:65591302-65591321 MsG0280010171.01.T01:CDS 45.0%
AGCGCGTAAAGTATAGGGTT+GGG + Chr2:65592170-65592189 None:intergenic 45.0%
ATGGTGAGCGCGTAAAGTAT+AGG + Chr2:65592176-65592195 None:intergenic 45.0%
CCAATTGCTGCAATTGTTCC+TGG + Chr2:65592620-65592639 None:intergenic 45.0%
CCAGGAACAATTGCAGCAAT+TGG - Chr2:65592617-65592636 MsG0280010171.01.T01:intron 45.0%
GAAGTGATTACACGCATGAG+AGG + Chr2:65592435-65592454 None:intergenic 45.0%
GAAGTTGAAGAAGAGGAGGA+AGG - Chr2:65591233-65591252 MsG0280010171.01.T01:exon 45.0%
GAGATATCATCGTCGCCATA+AGG - Chr2:65591385-65591404 MsG0280010171.01.T01:CDS 45.0%
GATCACACCTGCTACATTGT+TGG + Chr2:65591472-65591491 None:intergenic 45.0%
GCACGGACACTTATCGAATT+AGG - Chr2:65591525-65591544 MsG0280010171.01.T01:intron 45.0%
GCCAAATCAGATCAGAAGTG+GGG - Chr2:65594436-65594455 MsG0280010171.01.T01:intron 45.0%
GGAAGTCACTCATGTCACTA+TGG - Chr2:65593402-65593421 MsG0280010171.01.T01:intron 45.0%
TCTGATAAGCTTCCAAACGG+CGG + Chr2:65592224-65592243 None:intergenic 45.0%
TGGTGAGCGCGTAAAGTATA+GGG + Chr2:65592175-65592194 None:intergenic 45.0%
! AGTTGCTGACTGTGCAACTT+TGG - Chr2:65593925-65593944 MsG0280010171.01.T01:intron 45.0%
! GGTTTTGCCAACAATGTAGC+AGG - Chr2:65591462-65591481 MsG0280010171.01.T01:intron 45.0%
! TTCCAAAGCATCTGTTGTGG+TGG - Chr2:65591421-65591440 MsG0280010171.01.T01:CDS 45.0%
! TTTCCAAAGAGTGTGAGAGC+AGG - Chr2:65593250-65593269 MsG0280010171.01.T01:intron 45.0%
!! CATTGGTTCCTTACATGCCA+AGG - Chr2:65594984-65595003 MsG0280010171.01.T01:CDS 45.0%
!! CTACTAGCGGAAAGTGCTTA+GGG + Chr2:65591735-65591754 None:intergenic 45.0%
!! TCTACTAGCGGAAAGTGCTT+AGG + Chr2:65591736-65591755 None:intergenic 45.0%
!! AAAAAAAAAAAAACAATAAA+TGG - Chr2:65592719-65592738 MsG0280010171.01.T01:intron 5.0%
!! TATTATATATATATATATAG+GGG + Chr2:65592872-65592891 None:intergenic 5.0%
!!! AATTATTTTTTTTTAAAATC+TGG - Chr2:65594131-65594150 MsG0280010171.01.T01:intron 5.0%
!!! TAAAAAAAATTGTTTTAAAA+TGG - Chr2:65593855-65593874 MsG0280010171.01.T01:intron 5.0%
!!! TTAAAACAATTTTTTTTATA+GGG + Chr2:65593853-65593872 None:intergenic 5.0%
!!! TTGTTTTTTTTTTTTTTAAT+CGG + Chr2:65592715-65592734 None:intergenic 5.0%
!!! TTTAAAACAATTTTTTTTAT+AGG + Chr2:65593854-65593873 None:intergenic 5.0%
AAACCTGCGCACACTCATCA+AGG + Chr2:65594725-65594744 None:intergenic 50.0%
AAACCTTGATGAGTGTGCGC+AGG - Chr2:65594719-65594738 MsG0280010171.01.T01:CDS 50.0%
ACTGCTGGAACTTGCACTGA+AGG - Chr2:65594826-65594845 MsG0280010171.01.T01:CDS 50.0%
ACTGTGCAACTTTGGCCATG+TGG - Chr2:65593933-65593952 MsG0280010171.01.T01:intron 50.0%
AGAGAGAAGGGTTGGGACTT+TGG - Chr2:65592647-65592666 MsG0280010171.01.T01:intron 50.0%
ATCAGTGTCTGTGTCGTGTC+CGG - Chr2:65591652-65591671 MsG0280010171.01.T01:intron 50.0%
CATCTGTTGTGGTGGTTGCA+GGG - Chr2:65591429-65591448 MsG0280010171.01.T01:CDS 50.0%
GAATCGCAAGAGAGAAGGGT+TGG - Chr2:65592639-65592658 MsG0280010171.01.T01:intron 50.0%
GAGCAGGTCCTTCATCTTCA+TGG - Chr2:65593266-65593285 MsG0280010171.01.T01:intron 50.0%
GAGCGCGTAAAGTATAGGGT+TGG + Chr2:65592171-65592190 None:intergenic 50.0%
GCCCCACTTCTGATCTGATT+TGG + Chr2:65594440-65594459 None:intergenic 50.0%
GCGCGTAAAGTATAGGGTTG+GGG + Chr2:65592169-65592188 None:intergenic 50.0%
GCTTAGCGCGACACAATGAA+CGG - Chr2:65594791-65594810 MsG0280010171.01.T01:CDS 50.0%
GGAACTTGCACTGAAGGAAG+CGG - Chr2:65594832-65594851 MsG0280010171.01.T01:CDS 50.0%
GGGAGAAAAGAGCCAAGTAG+AGG - Chr2:65591299-65591318 MsG0280010171.01.T01:CDS 50.0%
GTTGAAGAAGAGGAGGAAGG+TGG - Chr2:65591236-65591255 MsG0280010171.01.T01:exon 50.0%
TCGTCGCCATAAGGTTTGTG+AGG - Chr2:65591394-65591413 MsG0280010171.01.T01:CDS 50.0%
TTGGCATGTTTCTTGCCACG+TGG - Chr2:65593901-65593920 MsG0280010171.01.T01:intron 50.0%
TTGTGGTGGTTGCAGGGATA+AGG - Chr2:65591435-65591454 MsG0280010171.01.T01:intron 50.0%
ACGCGCTCACCATGAGTATG+CGG - Chr2:65592183-65592202 MsG0280010171.01.T01:intron 55.0%
ATCAGATCAGAAGTGGGGCC+AGG - Chr2:65594441-65594460 MsG0280010171.01.T01:intron 55.0%
CGAAATGCGGATACGGACAC+CGG + Chr2:65591674-65591693 None:intergenic 55.0%
GAGCTTGCACCGCATACTCA+TGG + Chr2:65592195-65592214 None:intergenic 55.0%
GCATCTGTTGTGGTGGTTGC+AGG - Chr2:65591428-65591447 MsG0280010171.01.T01:CDS 55.0%
GGACCTGCTCTCACACTCTT+TGG + Chr2:65593256-65593275 None:intergenic 55.0%
GTATGCGGTGCAAGCTCAGA+CGG - Chr2:65592198-65592217 MsG0280010171.01.T01:intron 55.0%
TGCACTGAAGGAAGCGGTAC+TGG - Chr2:65594838-65594857 MsG0280010171.01.T01:CDS 55.0%
TGGTTGCAGGGATAAGGCAG+AGG - Chr2:65591441-65591460 MsG0280010171.01.T01:intron 55.0%
TTGGCATGGTTCTTGCCACG+TGG + Chr2:65594421-65594440 None:intergenic 55.0%
! TGAGGTTGTGAGGCATCTCC+AGG - Chr2:65592599-65592618 MsG0280010171.01.T01:intron 55.0%
CACAGTCAGCAACTGCCACG+TGG + Chr2:65593919-65593938 None:intergenic 60.0%
CATGCCACGTCATCGCCACA+TGG + Chr2:65593951-65593970 None:intergenic 60.0%
TTGGCCATGTGGCGATGACG+TGG - Chr2:65593944-65593963 MsG0280010171.01.T01:intron 60.0%
!! CGGCGTCGTAAAAGCACTGC+TGG - Chr2:65594811-65594830 MsG0280010171.01.T01:CDS 60.0%
AAGCTCAGACGGCCGCCGTT+TGG - Chr2:65592209-65592228 MsG0280010171.01.T01:intron 65.0%
ACTGCCATGTCAGCGCCACG+TGG - Chr2:65594403-65594422 MsG0280010171.01.T01:intron 65.0%
!! CTTGCCACGTGGCGCTGACA+TGG + Chr2:65594410-65594429 None:intergenic 65.0%
Chromosome Type Strat End Strand Name
Chr2 gene 65591155 65595034 65591155 ID=MsG0280010171.01;Name=MsG0280010171.01
Chr2 mRNA 65591155 65595034 65591155 ID=MsG0280010171.01.T01;Parent=MsG0280010171.01;Name=MsG0280010171.01.T01;_AED=0.47;_eAED=0.47;_QI=0|1|0.5|1|1|1|2|89|177
Chr2 exon 65591155 65591448 65591155 ID=MsG0280010171.01.T01:exon:5112;Parent=MsG0280010171.01.T01
Chr2 exon 65594706 65595034 65594706 ID=MsG0280010171.01.T01:exon:5111;Parent=MsG0280010171.01.T01
Chr2 CDS 65594706 65595034 65594706 ID=MsG0280010171.01.T01:cds;Parent=MsG0280010171.01.T01
Chr2 CDS 65591244 65591448 65591244 ID=MsG0280010171.01.T01:cds;Parent=MsG0280010171.01.T01
Chr2 three_prime_UTR 65591155 65591243 65591155 ID=MsG0280010171.01.T01:three_prime_utr;Parent=MsG0280010171.01.T01
Gene Sequence

>MsG0280010171.01.T01

ATGGAAGCAAGGAATACAATAGAGGGGAAGCAAACATCAGTTGAGAAGGTTTGGAAAGACGTCTTATTTGAAGAAATAGAAGTTGAAGAAGAGGAGGAAGGTGGTGTTGAAGATGATGAGAAGAAGAAAGGTGTTTGTGTGAGTGGGAGAAAAGAGCCAAGTAGAGGTGGAAGATTTTCTCCACCTTTTTGTCAAGTAGAAAGTTGTGGAGCTGATTTAACTTTTGCAAAGAGATATCATCGTCGCCATAAGGTTTGTGAGGTTCATTCCAAAGCATCTGTTGTGGTGGTTGCAGGGATAAGGCAGAGGTTTTGCCAACAATGTAGCAGGTTTCATGAGCTTGCAGAGTTTGATGAATCGAAAAGAAGCTGTCGAAGACGCTTAGCGCGACACAATGAACGGCGTCGTAAAAGCACTGCTGGAACTTGCACTGAAGGAAGCGGTACTGGCCAGCAAAATCATGATGGTGATTGGAGACAAATTCATATGAACATAACAAGTAGTTCTGGTCACGATTTTCTGAATTTCAGATGA
Protein sequence

>MsG0280010171.01.T01

MEARNTIEGKQTSVEKVWKDVLFEEIEVEEEEEGGVEDDEKKKGVCVSGRKEPSRGGRFSPPFCQVESCGADLTFAKRYHRRHKVCEVHSKASVVVVAGIRQRFCQQCSRFHELAEFDESKRSCRRRLARHNERRRKSTAGTCTEGSGTGQQNHDGDWRQIHMNITSSSGHDFLNFR*