AlfalfaGEDB | MsG0280010293.01

M. sativa cultivar ZhongmuNo.1 / MsG0280010293.01

KEGG: K24345
GO: GO:0005509
PFAM: PF13833

Nr:

Query id	Subject id	identity %	alignment length	mis matches	q. start	q. end	s. start	s. end	e-value	bit score
MsG0280010293.01.T02	XP_003596520.1	95.37	108	5	1	108	7	114	1.89E-67	208
MsG0280010293.01.T01	XP_003596520.1	95.614	114	5	1	114	1	114	1.51E-71	219
MsG0280010293.01.T03	XP_003596520.1	95.96	99	4	1	99	16	114	9.42E-61	191

Swissprot:

Query id	Subject id	identity %	alignment length	mis matches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MsG0280010293.01.T01	Q9LSQ6	56.842	95	39	2	9	101	19	113	4.61E-31	109
MsG0280010293.01.T02	Q9LSQ6	56.842	95	39	2	3	95	19	113	3.02E-31	110
MsG0280010293.01.T03	Q9LSQ6	56.818	88	36	2	1	86	26	113	1.18E-26	97.8

Trembl:

Query id	Subject id	identity %	alignment length	mis matches	q. start	q. end	s. start	s. end	e-value	bit score
MsG0280010293.01.T01	Q2HVR3	95.614	114	5	1	114	1	114	7.22e-72	219
MsG0280010293.01.T02	Q2HVR3	95.370	108	5	1	108	7	114	9.02e-68	208
MsG0280010293.01.T03	Q2HVR3	95.960	99	4	1	99	16	114	4.50e-61	191

TFs/TRs:

Gene ID	Type	Classification

Protein Kinases:

Gene ID	Type	Classification

Network:

Co-expression Network

Gene1	Gene2	correlation coefficient	p_value	FDR
MsG0180000632.01	MsG0280010293.01	0.817238	3.658541e-52	2.649704e-49
MsG0280010292.01	MsG0280010293.01	0.813464	2.526625e-51	1.651252e-48
MsG0280010293.01	MsG0680033933.01	0.805149	1.534411e-49	8.057929e-47

PPI

Gene1	Gene2	Type

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MsG0280010293.01.T02	MTR_2g081440	95.370	108	5	0	1	108	7	114	2.29e-71	208
MsG0280010293.01.T02	MTR_2g081350	89.815	108	11	0	1	108	7	114	5.96e-65	192
MsG0280010293.01.T02	MTR_2g081300	86.111	108	15	0	1	108	3	110	9.69e-65	191
MsG0280010293.01.T02	MTR_2g011400	63.830	94	33	1	3	95	14	107	4.43e-36	119
MsG0280010293.01.T02	MTR_8g466250	61.458	96	36	1	3	97	11	106	1.40e-34	115
MsG0280010293.01.T02	MTR_2g081580	61.053	95	36	1	3	96	10	104	1.67e-34	115
MsG0280010293.01.T02	MTR_2g089210	58.065	93	38	1	1	92	8	100	1.22e-32	110
MsG0280010293.01.T02	MTR_1g052885	59.341	91	36	1	3	92	6	96	5.38e-31	106
MsG0280010293.01.T02	MTR_8g027180	54.348	92	41	1	2	92	18	109	1.03e-27	98.6
MsG0280010293.01.T01	MTR_2g081440	95.614	114	5	0	1	114	1	114	1.83e-75	219
MsG0280010293.01.T01	MTR_2g081350	88.596	114	13	0	1	114	1	114	3.48e-67	198
MsG0280010293.01.T01	MTR_2g081300	85.321	109	16	0	6	114	2	110	9.21e-65	191
MsG0280010293.01.T01	MTR_2g011400	63.830	94	33	1	9	101	14	107	8.36e-36	119
MsG0280010293.01.T01	MTR_2g081580	59.794	97	38	1	7	102	8	104	2.17e-34	115
MsG0280010293.01.T01	MTR_8g466250	61.458	96	36	1	9	103	11	106	2.40e-34	115
MsG0280010293.01.T01	MTR_2g089210	56.122	98	42	1	2	98	3	100	1.61e-32	110
MsG0280010293.01.T01	MTR_1g052885	59.341	91	36	1	9	98	6	96	8.77e-31	106
MsG0280010293.01.T01	MTR_8g027180	53.191	94	43	1	6	98	16	109	1.07e-27	98.6
MsG0280010293.01.T03	MTR_2g081440	95.960	99	4	0	1	99	16	114	1.14e-64	191
MsG0280010293.01.T03	MTR_2g081350	88.889	99	11	0	1	99	16	114	6.75e-58	174
MsG0280010293.01.T03	MTR_2g081300	85.859	99	14	0	1	99	12	110	9.85e-58	173
MsG0280010293.01.T03	MTR_8g466250	62.921	89	32	1	1	88	18	106	2.89e-32	109
MsG0280010293.01.T03	MTR_2g081580	61.798	89	33	1	1	88	17	105	6.21e-32	108
MsG0280010293.01.T03	MTR_2g011400	60.920	87	33	1	1	86	21	107	7.38e-30	103
MsG0280010293.01.T03	MTR_2g089210	60.494	81	31	1	4	83	20	100	3.14e-28	99.0
MsG0280010293.01.T03	MTR_1g052885	59.524	84	33	1	1	83	13	96	1.58e-27	97.1
MsG0280010293.01.T03	MTR_8g027180	54.762	84	37	1	1	83	26	109	2.50e-24	89.4

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MsG0280010293.01.T02	AT5G54490	56.842	95	39	2	3	95	19	113	3.08e-32	110
MsG0280010293.01.T02	AT4G27280	55.208	96	41	2	2	95	18	113	1.26e-29	103
MsG0280010293.01.T02	AT2G46600	50.549	91	44	1	3	92	22	112	4.65e-25	92.0
MsG0280010293.01.T01	AT5G54490	56.842	95	39	2	9	101	19	113	4.70e-32	109
MsG0280010293.01.T01	AT4G27280	55.208	96	41	2	8	101	18	113	1.85e-29	103
MsG0280010293.01.T01	AT2G46600	48.936	94	47	1	6	98	19	112	6.66e-25	92.0
MsG0280010293.01.T03	AT5G54490	56.818	88	36	2	1	86	26	113	1.20e-27	97.8
MsG0280010293.01.T03	AT4G27280	56.818	88	36	2	1	86	26	113	7.61e-26	93.6
MsG0280010293.01.T03	AT2G46600	51.190	84	40	1	1	83	29	112	2.61e-22	84.7

Find 28 sgRNAs with CRISPR-Local

Find 29 sgRNAs with CRISPR-GE

CRISPR-Local

CRISPR-Local
sgRNA_sequence	on_target_score	Position	Region
CTACGGGTTTCATTTGCTTA+TGG	0.204248	2:-67490557	MsG0280010293.01.T01:CDS
CCATCCATGGTTGCGAGTTT+AGG	0.278334	2:-67490607	MsG0280010293.01.T01:CDS
TTAGGAGCAGAAGCATTTAT+AGG	0.308592	2:-67490589	MsG0280010293.01.T01:CDS
CATGCTAATGGAAGGTGATT+TGG	0.335601	2:-67490446	MsG0280010293.01.T01:CDS
AAAATATGCACAAATCTTCA+AGG	0.428589	2:-67490346	MsG0280010293.01.T01:CDS
TAGGTTGAGTCCATGTTTAA+TGG	0.441618	2:-67490377	MsG0280010293.01.T01:CDS
CCTAAACTCGCAACCATGGA+TGG	0.475656	2:+67490607	None:intergenic
CACAAGTTTCCACATGGAGT+TGG	0.491047	2:-67490659	None:intergenic
AAGATGAATTGTTTCATGCT+TGG	0.497368	2:-67490499	MsG0280010293.01.T01:CDS
TGAGGACTACTTTCCATCCA+TGG	0.498192	2:-67490620	MsG0280010293.01.T01:CDS
TGAGCTCGTGTACATGCTAA+TGG	0.499730	2:-67490458	MsG0280010293.01.T01:CDS
ATGAGAATGCAAAACTCCAT+TGG	0.509723	2:+67490403	None:intergenic
GAATTGTTTCATGCTTGGTA+TGG	0.522428	2:-67490494	MsG0280010293.01.T01:CDS
TTGAGTCCATGTTTAATGGA+TGG	0.531601	2:-67490373	MsG0280010293.01.T01:CDS
TTTATAGGTGAACTTTGCTA+CGG	0.531654	2:-67490574	MsG0280010293.01.T01:CDS
TTATAGGTGAACTTTGCTAC+GGG	0.538698	2:-67490573	MsG0280010293.01.T01:CDS
TTTGCTTATGGATGTCAACA+AGG	0.557910	2:-67490545	MsG0280010293.01.T01:CDS
TCATGCTTGGTATGGAGGTG+AGG	0.562351	2:-67490486	MsG0280010293.01.T01:CDS
CATGCTTGGTATGGAGGTGA+GGG	0.564464	2:-67490485	MsG0280010293.01.T01:CDS
AGATGGTGCTTTAAATCCAA+TGG	0.565219	2:-67490419	MsG0280010293.01.T01:CDS
TTGTTTCATGCTTGGTATGG+AGG	0.565973	2:-67490491	MsG0280010293.01.T01:CDS
TGCTCCTAAACTCGCAACCA+TGG	0.581169	2:+67490603	None:intergenic
GAGTGTCATCACATTAACAT+TGG	0.590328	2:+67490316	None:intergenic
TTAGGTCCATCCATTAAACA+TGG	0.608146	2:+67490367	None:intergenic
GAAGGTGATTTGGATGGAGA+TGG	0.627275	2:-67490436	MsG0280010293.01.T01:CDS
TTGCTTATGGATGTCAACAA+GGG	0.661501	2:-67490544	MsG0280010293.01.T01:CDS
CTAATGGAAGGTGATTTGGA+TGG	0.674005	2:-67490442	MsG0280010293.01.T01:CDS
CTCGTGTACATGCTAATGGA+AGG	0.692064	2:-67490454	MsG0280010293.01.T01:CDS

CRISPR-GE

badsite warning	sgRNA_sequence	Strand	Position	Region	GC_content
!	AAAATATGCACAAATCTTCA+AGG	-	Chr2:67490624-67490643	MsG0280010293.01.T01:CDS	25.0%
!!	TGAAGATTTGTGCATATTTT+AGG	+	Chr2:67490624-67490643	None:intergenic	25.0%
	AAGATGAATTGTTTCATGCT+TGG	-	Chr2:67490471-67490490	MsG0280010293.01.T01:CDS	30.0%
	TTTATAGGTGAACTTTGCTA+CGG	-	Chr2:67490396-67490415	MsG0280010293.01.T01:CDS	30.0%
!	AGTTTTGCATTCTCATGTTT+AGG	-	Chr2:67490574-67490593	MsG0280010293.01.T01:CDS	30.0%
!!	GGATTTTGATATGGATTTTG+AGG	-	Chr2:67490332-67490351	MsG0280010293.01.T01:CDS	30.0%
	ATGAGAATGCAAAACTCCAT+TGG	+	Chr2:67490570-67490589	None:intergenic	35.0%
	GAATTGTTTCATGCTTGGTA+TGG	-	Chr2:67490476-67490495	MsG0280010293.01.T01:CDS	35.0%
	TTAGGTCCATCCATTAAACA+TGG	+	Chr2:67490606-67490625	None:intergenic	35.0%
	TTATAGGTGAACTTTGCTAC+GGG	-	Chr2:67490397-67490416	MsG0280010293.01.T01:CDS	35.0%
	TTGAGTCCATGTTTAATGGA+TGG	-	Chr2:67490597-67490616	MsG0280010293.01.T01:CDS	35.0%
	TTGCTTATGGATGTCAACAA+GGG	-	Chr2:67490426-67490445	MsG0280010293.01.T01:CDS	35.0%
	TTTGCTTATGGATGTCAACA+AGG	-	Chr2:67490425-67490444	MsG0280010293.01.T01:CDS	35.0%
!	AGATGGTGCTTTAAATCCAA+TGG	-	Chr2:67490551-67490570	MsG0280010293.01.T01:CDS	35.0%
!	TAGGTTGAGTCCATGTTTAA+TGG	-	Chr2:67490593-67490612	MsG0280010293.01.T01:CDS	35.0%
!!	TTAGGAGCAGAAGCATTTAT+AGG	-	Chr2:67490381-67490400	MsG0280010293.01.T01:CDS	35.0%
	CATGCTAATGGAAGGTGATT+TGG	-	Chr2:67490524-67490543	MsG0280010293.01.T01:CDS	40.0%
	CTACGGGTTTCATTTGCTTA+TGG	-	Chr2:67490413-67490432	MsG0280010293.01.T01:CDS	40.0%
	TTGTTTCATGCTTGGTATGG+AGG	-	Chr2:67490479-67490498	MsG0280010293.01.T01:CDS	40.0%
!	CTAATGGAAGGTGATTTGGA+TGG	-	Chr2:67490528-67490547	MsG0280010293.01.T01:CDS	40.0%
	CTCGTGTACATGCTAATGGA+AGG	-	Chr2:67490516-67490535	MsG0280010293.01.T01:CDS	45.0%
	TGAGCTCGTGTACATGCTAA+TGG	-	Chr2:67490512-67490531	MsG0280010293.01.T01:CDS	45.0%
	TGAGGACTACTTTCCATCCA+TGG	-	Chr2:67490350-67490369	MsG0280010293.01.T01:CDS	45.0%
!	GAAGGTGATTTGGATGGAGA+TGG	-	Chr2:67490534-67490553	MsG0280010293.01.T01:CDS	45.0%
	CATGCTTGGTATGGAGGTGA+GGG	-	Chr2:67490485-67490504	MsG0280010293.01.T01:CDS	50.0%
	CCATCCATGGTTGCGAGTTT+AGG	-	Chr2:67490363-67490382	MsG0280010293.01.T01:CDS	50.0%
	CCTAAACTCGCAACCATGGA+TGG	+	Chr2:67490366-67490385	None:intergenic	50.0%
	TCATGCTTGGTATGGAGGTG+AGG	-	Chr2:67490484-67490503	MsG0280010293.01.T01:CDS	50.0%
	TGCTCCTAAACTCGCAACCA+TGG	+	Chr2:67490370-67490389	None:intergenic	50.0%

Chromosome	Type	Strat	End	Strand	Name
Chr2	gene	67490324	67490668	67490324	ID=MsG0280010293.01;Name=MsG0280010293.01
Chr2	mRNA	67490324	67490668	67490324	ID=MsG0280010293.01.T01;Parent=MsG0280010293.01;Name=MsG0280010293.01.T01;_AED=0.05;_eAED=0.05;_QI=0\|-1\|0\|1\|-1\|1\|1\|0\|114
Chr2	exon	67490324	67490668	67490324	ID=MsG0280010293.01.T01:exon:18414;Parent=MsG0280010293.01.T01
Chr2	CDS	67490324	67490668	67490324	ID=MsG0280010293.01.T01:cds;Parent=MsG0280010293.01.T01
Chr2	mRNA	67490324	67490650	67490324	ID=MsG0280010293.01.T02;Parent=MsG0280010293.01;Name=MsG0280010293.01.T02;_AED=0.07;_eAED=0.07;_QI=0\|-1\|0\|1\|-1\|0\|1\|0\|108
Chr2	exon	67490324	67490650	67490324	ID=MsG0280010293.01.T02:exon:18415;Parent=MsG0280010293.01.T02
Chr2	CDS	67490324	67490650	67490324	ID=MsG0280010293.01.T02:cds;Parent=MsG0280010293.01.T02
Chr2	mRNA	67490324	67490623	67490324	ID=MsG0280010293.01.T03;Parent=MsG0280010293.01;Name=MsG0280010293.01.T03;_AED=0.11;_eAED=0.11;_QI=0\|-1\|0\|1\|-1\|0\|1\|0\|99
Chr2	exon	67490324	67490623	67490324	ID=MsG0280010293.01.T03:exon:18416;Parent=MsG0280010293.01.T03
Chr2	CDS	67490324	67490623	67490324	ID=MsG0280010293.01.T03:cds;Parent=MsG0280010293.01.T03

Gene Sequence

>MsG0280010293.01.T02

ATGGATTTTGAGGACTACTTTCCATCCATGGTTGCGAGTTTAGGAGCAGAAGCATTTATAGGTGAACTTTGCTACGGGTTTCATTTGCTTATGGATGTCAACAAGGGTCTTATAACATTTGAGAGTTTGAAGATGAATTGTTTCATGCTTGGTATGGAGGTGAGGGATGATGAGCTCGTGTACATGCTAATGGAAGGTGATTTGGATGGAGATGGTGCTTTAAATCCAATGGAGTTTTGCATTCTCATGTTTAGGTTGAGTCCATGTTTAATGGATGGACCTAAAATATGCACAAATCTTCAAGGTGTGAATCCAATGTTAATGTGA

>MsG0280010293.01.T01

ATGGAGTTGGATTTTGATATGGATTTTGAGGACTACTTTCCATCCATGGTTGCGAGTTTAGGAGCAGAAGCATTTATAGGTGAACTTTGCTACGGGTTTCATTTGCTTATGGATGTCAACAAGGGTCTTATAACATTTGAGAGTTTGAAGATGAATTGTTTCATGCTTGGTATGGAGGTGAGGGATGATGAGCTCGTGTACATGCTAATGGAAGGTGATTTGGATGGAGATGGTGCTTTAAATCCAATGGAGTTTTGCATTCTCATGTTTAGGTTGAGTCCATGTTTAATGGATGGACCTAAAATATGCACAAATCTTCAAGGTGTGAATCCAATGTTAATGTGA

>MsG0280010293.01.T03

ATGGTTGCGAGTTTAGGAGCAGAAGCATTTATAGGTGAACTTTGCTACGGGTTTCATTTGCTTATGGATGTCAACAAGGGTCTTATAACATTTGAGAGTTTGAAGATGAATTGTTTCATGCTTGGTATGGAGGTGAGGGATGATGAGCTCGTGTACATGCTAATGGAAGGTGATTTGGATGGAGATGGTGCTTTAAATCCAATGGAGTTTTGCATTCTCATGTTTAGGTTGAGTCCATGTTTAATGGATGGACCTAAAATATGCACAAATCTTCAAGGTGTGAATCCAATGTTAATGTGA

Protein sequence

>MsG0280010293.01.T02

MDFEDYFPSMVASLGAEAFIGELCYGFHLLMDVNKGLITFESLKMNCFMLGMEVRDDELVYMLMEGDLDGDGALNPMEFCILMFRLSPCLMDGPKICTNLQGVNPMLM*

>MsG0280010293.01.T01

MELDFDMDFEDYFPSMVASLGAEAFIGELCYGFHLLMDVNKGLITFESLKMNCFMLGMEVRDDELVYMLMEGDLDGDGALNPMEFCILMFRLSPCLMDGPKICTNLQGVNPMLM*

>MsG0280010293.01.T03

MVASLGAEAFIGELCYGFHLLMDVNKGLITFESLKMNCFMLGMEVRDDELVYMLMEGDLDGDGALNPMEFCILMFRLSPCLMDGPKICTNLQGVNPMLM*