AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar ZhongmuNo.1 / MsG0180000632.01


Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MsG0180000632.01.T01 MTR_1g011630 77.273 242 24 2 1 211 93 334 1.13e-128 369
MsG0180000632.01.T01 MTR_6g086870 46.185 249 96 3 1 211 97 345 3.24e-69 217
MsG0180000632.01.T01 MTR_1g008380 47.177 248 97 3 1 214 119 366 7.25e-68 214
MsG0180000632.01.T01 MTR_3g109520 45.528 246 99 4 1 211 116 361 1.19e-65 208
MsG0180000632.01.T01 MTR_7g099330 42.798 243 105 4 1 211 101 341 1.79e-64 205
MsG0180000632.01.T01 MTR_7g099160 42.798 243 105 4 1 211 222 462 9.08e-64 206
MsG0180000632.01.T01 MTR_1g069275 41.224 245 110 4 1 211 102 346 7.00e-61 196
MsG0180000632.01.T01 MTR_1g046230 40.726 248 110 3 1 211 101 348 1.59e-60 195
MsG0180000632.01.T01 MTR_8g432640 36.364 242 123 4 1 211 83 324 2.07e-41 144
MsG0180000632.01.T01 MTR_5g033260 35.124 242 126 4 1 211 83 324 1.48e-37 134
MsG0180000632.01.T01 MTR_7g107690 31.174 247 129 4 1 211 85 326 8.88e-37 132
MsG0180000632.01.T01 MTR_4g051642 33.198 247 124 5 1 211 84 325 1.76e-36 132
MsG0180000632.01.T01 MTR_3g088945 31.984 247 127 4 1 211 84 325 9.99e-36 130
MsG0180000632.01.T01 MTR_3g088925 31.984 247 127 4 1 211 84 325 1.01e-35 130
MsG0180000632.01.T01 MTR_7g107710 31.452 248 128 6 1 211 83 325 1.32e-33 124
MsG0180000632.01.T01 MTR_3g088955 30.769 247 130 4 1 211 84 325 4.27e-33 123
MsG0180000632.01.T01 MTR_3g096030 32.967 91 57 2 1 87 59 149 2.66e-12 62.8
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MsG0180000632.01.T01 AT5G11320 54.321 243 78 3 1 212 94 334 6.85e-82 248
MsG0180000632.01.T01 AT5G11320 54.132 242 78 3 1 211 94 333 6.58e-80 244
MsG0180000632.01.T01 AT4G32540 48.770 244 90 5 1 211 98 339 2.53e-72 225
MsG0180000632.01.T01 AT4G32540 48.374 246 90 5 1 211 98 341 4.48e-72 225
MsG0180000632.01.T01 AT4G13260 45.783 249 100 3 1 214 105 353 3.15e-68 215
MsG0180000632.01.T01 AT5G25620 46.341 246 97 4 1 211 109 354 1.21e-67 213
MsG0180000632.01.T01 AT5G25620 46.341 246 97 4 1 211 109 354 1.55e-67 214
MsG0180000632.01.T01 AT4G13260 44.628 242 99 3 8 214 9 250 1.81e-63 199
MsG0180000632.01.T01 AT1G04180 45.714 245 99 4 1 211 102 346 2.82e-63 202
MsG0180000632.01.T01 AT1G04610 42.169 249 106 3 1 211 114 362 1.30e-62 201
MsG0180000632.01.T01 AT2G33230 43.373 249 103 3 1 211 109 357 1.25e-61 198
MsG0180000632.01.T01 AT4G28720 40.323 248 111 3 1 211 102 349 2.68e-61 197
MsG0180000632.01.T01 AT5G43890 41.935 248 107 4 1 211 102 349 7.63e-60 193
MsG0180000632.01.T01 AT4G13260 45.545 202 79 2 1 171 105 306 4.87e-57 183
MsG0180000632.01.T01 AT1G48910 35.391 243 124 5 1 211 82 323 5.12e-43 149
MsG0180000632.01.T01 AT1G21430 34.146 246 123 5 1 212 86 326 1.21e-38 137

Find 33 sgRNAs with CRISPR-Local

Find 93 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
TTGATTCAAGTTCTCAAATT+TGG 0.134264 1:+8927464 MsG0180000632.01.T01:CDS
GTTAGTTTCAAGCTTCTTCT+TGG 0.280395 1:+8928625 MsG0180000632.01.T01:CDS
TAGCTATGGGATTATATAAA+TGG 0.285172 1:+8928570 MsG0180000632.01.T01:CDS
GTTGCAAGTTTGAGTTCTAT+TGG 0.313027 1:-8928689 None:intergenic
ATGTTTGGTTTCTCAACATA+TGG 0.323559 1:+8928545 MsG0180000632.01.T01:CDS
CATACAAGTGATTATAAATC+TGG 0.355020 1:+8927613 MsG0180000632.01.T01:CDS
GATGCAATAATCTTAGCAAC+AGG 0.380414 1:+8928943 MsG0180000632.01.T01:CDS
TAATCACTTGTATGAGCAAC+AGG 0.388008 1:-8927604 None:intergenic
CCTTGGCTTATTGTTGCTAC+TGG 0.412409 1:+8927526 MsG0180000632.01.T01:CDS
TTAGTTTCAAGCTTCTTCTT+GGG 0.427173 1:+8928626 MsG0180000632.01.T01:CDS
TGTTCCATACCATGAATTGT+TGG 0.436540 1:-8927571 None:intergenic
ATTCTTCCAAGAGATATGTT+TGG 0.437726 1:+8928530 MsG0180000632.01.T01:CDS
GGTATCAAGAGGCCTAAAAC+TGG 0.451158 1:+8928665 MsG0180000632.01.T01:CDS
ACAAGAGCAATGTGCCTACT+TGG 0.453063 1:+8928968 MsG0180000632.01.T01:CDS
GAGAAACCAAACATATCTCT+TGG 0.462714 1:-8928536 None:intergenic
TTGGGAAACACCAATCACTA+TGG 0.465852 1:+8928644 MsG0180000632.01.T01:CDS
ATAGAACTCAAACTTGCAAC+AGG 0.466421 1:+8928692 MsG0180000632.01.T01:CDS
CAAATAGCACAAATTAAATC+CGG 0.476052 1:+8928740 MsG0180000632.01.T01:CDS
GTGAAAGAGATAACAAGAAA+TGG 0.481980 1:+8928887 MsG0180000632.01.T01:CDS
ATCACTTTGATATTCATCCT+AGG 0.490915 1:+8927413 MsG0180000632.01.T01:CDS
CCTCTTGATACCATAGTGAT+TGG 0.496998 1:-8928654 None:intergenic
CTCAACATATGGAATAGCTA+TGG 0.500774 1:+8928556 MsG0180000632.01.T01:CDS
TCAACATATGGAATAGCTAT+GGG 0.521973 1:+8928557 MsG0180000632.01.T01:CDS
TTCAAGTTCTCAAATTTGGA+TGG 0.536810 1:+8927468 MsG0180000632.01.T01:CDS
AAAACTCCTGTACTTGATGT+TGG 0.551364 1:+8928716 MsG0180000632.01.T01:CDS
AATATCAAAGTGATCAGCAT+AGG 0.584130 1:-8927404 None:intergenic
ATTTGACCAACATCAAGTAC+AGG 0.585749 1:-8928722 None:intergenic
CAATGTGCCTACTTGGCTCA+AGG 0.592907 1:+8928975 MsG0180000632.01.T01:CDS
AGAACAGTTTGATTAAACCT+AGG 0.595686 1:-8927430 None:intergenic
TTAATATTGTAGGTGATGGA+AGG 0.640132 1:+8928863 MsG0180000632.01.T01:intron
CCAATCACTATGGTATCAAG+AGG 0.641108 1:+8928654 MsG0180000632.01.T01:CDS
CCAGTAGCAACAATAAGCCA+AGG 0.647996 1:-8927526 None:intergenic
ATGCAATAATCTTAGCAACA+GGG 0.685349 1:+8928944 MsG0180000632.01.T01:CDS

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!!! TTTTATATTAATAATTTATT+AGG + Chr1:8928785-8928804 MsG0180000632.01.T01:intron 0.0%
!! TGATATTAAAAAAAAAAATG+CGG + Chr1:8927794-8927813 MsG0180000632.01.T01:intron 10.0%
!!! TTTGATATGTATATTTTTTT+AGG + Chr1:8928820-8928839 MsG0180000632.01.T01:intron 10.0%
!! AAAGAAATATTAAATGATGA+AGG - Chr1:8928128-8928147 None:intergenic 15.0%
!! ATAAATAATCAAGGTAATAA+AGG + Chr1:8928167-8928186 MsG0180000632.01.T01:intron 15.0%
!! ATTATTAAAATTACCCAAAT+GGG - Chr1:8928445-8928464 None:intergenic 15.0%
!!! GCAATATTTTATATGATTTA+AGG + Chr1:8928502-8928521 MsG0180000632.01.T01:intron 15.0%
!! AAATGACAATTAAAAAGGAA+CGG + Chr1:8928258-8928277 MsG0180000632.01.T01:intron 20.0%
!! ATTGAAATTCGAAAGAAAAA+AGG - Chr1:8927760-8927779 None:intergenic 20.0%
!! ATTGTTTAGTACTTAAAATG+AGG + Chr1:8928399-8928418 MsG0180000632.01.T01:intron 20.0%
!! GATTATTAAAATTACCCAAA+TGG - Chr1:8928446-8928465 None:intergenic 20.0%
!! TTGAAATTCGAAAGAAAAAA+GGG - Chr1:8927759-8927778 None:intergenic 20.0%
!!! AACTTGTCTACTAATTTTAA+TGG - Chr1:8928599-8928618 None:intergenic 20.0%
!!! AGTACAAAAATAAAAGGTTT+TGG + Chr1:8927641-8927660 MsG0180000632.01.T01:CDS 20.0%
!!! ATATGTTATTTTTTCCCATT+TGG + Chr1:8928428-8928447 MsG0180000632.01.T01:intron 20.0%
!!! TATGTATATTTTTTTAGGCT+AGG + Chr1:8928825-8928844 MsG0180000632.01.T01:intron 20.0%
!!! TATGTTATTTTTTCCCATTT+GGG + Chr1:8928429-8928448 MsG0180000632.01.T01:intron 20.0%
! AAAAAAAATGCGGTTTAATG+TGG + Chr1:8927804-8927823 MsG0180000632.01.T01:intron 25.0%
! AAATCACGACAAAAAAATGA+TGG - Chr1:8927955-8927974 None:intergenic 25.0%
! AATTAAATCCGGTAACATTA+AGG + Chr1:8928751-8928770 MsG0180000632.01.T01:intron 25.0%
! CAAATAGCACAAATTAAATC+CGG + Chr1:8928740-8928759 MsG0180000632.01.T01:CDS 25.0%
! CATACAAGTGATTATAAATC+TGG + Chr1:8927613-8927632 MsG0180000632.01.T01:CDS 25.0%
! CTGCAAAATGACAATTAAAA+AGG + Chr1:8928253-8928272 MsG0180000632.01.T01:intron 25.0%
! TAGCTATGGGATTATATAAA+TGG + Chr1:8928570-8928589 MsG0180000632.01.T01:CDS 25.0%
! TTAATACATGTTTCGATTCA+TGG + Chr1:8927886-8927905 MsG0180000632.01.T01:intron 25.0%
! TTGATTAATATTGTAGGTGA+TGG + Chr1:8928859-8928878 MsG0180000632.01.T01:intron 25.0%
! TTGGGATTGATTAATATTGT+AGG + Chr1:8928853-8928872 MsG0180000632.01.T01:intron 25.0%
! TTTAATAAGGTCACTCATAA+AGG + Chr1:8928335-8928354 MsG0180000632.01.T01:intron 25.0%
!! AAATGGTGCAAAATTTTTGA+TGG + Chr1:8928904-8928923 MsG0180000632.01.T01:CDS 25.0%
!! ATTTTCAGTATTTTTCACCT+TGG + Chr1:8927509-8927528 MsG0180000632.01.T01:CDS 25.0%
!! GTTCTGAGTACAAAAATAAA+AGG + Chr1:8927635-8927654 MsG0180000632.01.T01:CDS 25.0%
!! TTGATTCAAGTTCTCAAATT+TGG + Chr1:8927464-8927483 MsG0180000632.01.T01:CDS 25.0%
!!! AAATAAAAGGTTTTGGTCAT+TGG + Chr1:8927648-8927667 MsG0180000632.01.T01:CDS 25.0%
!!! AATTTTTGATGGACAAGAAA+AGG + Chr1:8928915-8928934 MsG0180000632.01.T01:CDS 25.0%
!!! TTTTTAGGCTAGGATTAATT+GGG + Chr1:8928835-8928854 MsG0180000632.01.T01:intron 25.0%
!!! TTTTTTAGGCTAGGATTAAT+TGG + Chr1:8928834-8928853 MsG0180000632.01.T01:intron 25.0%
AAAGTGACCATTAAAAGGAA+CGG - Chr1:8928026-8928045 None:intergenic 30.0%
AATATCAAAGTGATCAGCAT+AGG - Chr1:8927407-8927426 None:intergenic 30.0%
AGAACAGTTTGATTAAACCT+AGG - Chr1:8927433-8927452 None:intergenic 30.0%
ATCACTTTGATATTCATCCT+AGG + Chr1:8927413-8927432 MsG0180000632.01.T01:CDS 30.0%
ATGCAATAATCTTAGCAACA+GGG + Chr1:8928944-8928963 MsG0180000632.01.T01:CDS 30.0%
ATTCTTCCAAGAGATATGTT+TGG + Chr1:8928530-8928549 MsG0180000632.01.T01:CDS 30.0%
CTCTCTGCAATAAATAATCA+AGG + Chr1:8928158-8928177 MsG0180000632.01.T01:intron 30.0%
GTAACATACCTTAATGTTAC+CGG - Chr1:8928762-8928781 None:intergenic 30.0%
GTGAAAGAGATAACAAGAAA+TGG + Chr1:8928887-8928906 MsG0180000632.01.T01:CDS 30.0%
TCAACATATGGAATAGCTAT+GGG + Chr1:8928557-8928576 MsG0180000632.01.T01:CDS 30.0%
TTAGTTTCAAGCTTCTTCTT+GGG + Chr1:8928626-8928645 MsG0180000632.01.T01:CDS 30.0%
! ATGTTTGGTTTCTCAACATA+TGG + Chr1:8928545-8928564 MsG0180000632.01.T01:CDS 30.0%
! TTCAAGTTCTCAAATTTGGA+TGG + Chr1:8927468-8927487 MsG0180000632.01.T01:CDS 30.0%
! TTTTCCAACAATTCATGGTA+TGG + Chr1:8927567-8927586 MsG0180000632.01.T01:CDS 30.0%
!! ACTTGTGAGTTTGTTCTTTT+TGG + Chr1:8928468-8928487 MsG0180000632.01.T01:intron 30.0%
!! TAGCTAGAACTTTGAAATGA+CGG - Chr1:8927989-8928008 None:intergenic 30.0%
!! TTAATATTGTAGGTGATGGA+AGG + Chr1:8928863-8928882 MsG0180000632.01.T01:intron 30.0%
!! TTCCTTTTAATGGTCACTTT+TGG + Chr1:8928026-8928045 MsG0180000632.01.T01:intron 30.0%
!!! TCCTTTTAATGGTCACTTTT+GGG + Chr1:8928027-8928046 MsG0180000632.01.T01:intron 30.0%
!!! TGATGGTTTTGACAAAATTG+CGG - Chr1:8927938-8927957 None:intergenic 30.0%
!!! TTGTTCTTTTTGGCTAACTT+TGG + Chr1:8928478-8928497 MsG0180000632.01.T01:intron 30.0%
AAAACTCCTGTACTTGATGT+TGG + Chr1:8928716-8928735 MsG0180000632.01.T01:CDS 35.0%
ACCCAAAAGTGACCATTAAA+AGG - Chr1:8928031-8928050 None:intergenic 35.0%
ATAGAACTCAAACTTGCAAC+AGG + Chr1:8928692-8928711 MsG0180000632.01.T01:CDS 35.0%
ATTTGACCAACATCAAGTAC+AGG - Chr1:8928725-8928744 None:intergenic 35.0%
CCATGAATTGTTGGAAAAAC+AGG - Chr1:8927565-8927584 None:intergenic 35.0%
CTCAACATATGGAATAGCTA+TGG + Chr1:8928556-8928575 MsG0180000632.01.T01:CDS 35.0%
GAGAAACCAAACATATCTCT+TGG - Chr1:8928539-8928558 None:intergenic 35.0%
GATGCAATAATCTTAGCAAC+AGG + Chr1:8928943-8928962 MsG0180000632.01.T01:CDS 35.0%
TAATCACTTGTATGAGCAAC+AGG - Chr1:8927607-8927626 None:intergenic 35.0%
TGACAATTAAAAAGGAACGG+AGG + Chr1:8928261-8928280 MsG0180000632.01.T01:intron 35.0%
TGTTCCATACCATGAATTGT+TGG - Chr1:8927574-8927593 None:intergenic 35.0%
TTTGACAAAATTGCGGTCAT+AGG - Chr1:8927931-8927950 None:intergenic 35.0%
! AGTTCTATTGGACCAGTTTT+AGG - Chr1:8928680-8928699 None:intergenic 35.0%
! CACATCGATTAAAGATGCAT+CGG - Chr1:8927830-8927849 None:intergenic 35.0%
! CATGGTATGGAACATTTTCA+TGG + Chr1:8927580-8927599 MsG0180000632.01.T01:CDS 35.0%
! GTTAGTTTCAAGCTTCTTCT+TGG + Chr1:8928625-8928644 MsG0180000632.01.T01:CDS 35.0%
! GTTGCAAGTTTGAGTTCTAT+TGG - Chr1:8928692-8928711 None:intergenic 35.0%
! TTTTTAACCTTGAGCCAAGT+AGG - Chr1:8928985-8929004 None:intergenic 35.0%
!! CCTGTTTTTCCAACAATTCA+TGG + Chr1:8927562-8927581 MsG0180000632.01.T01:CDS 35.0%
!! TTCTGGAATGGAAGTTAGTT+TGG + Chr1:8927679-8927698 MsG0180000632.01.T01:intron 35.0%
ACACAATGCTATGCCTCATT+TGG + Chr1:8927712-8927731 MsG0180000632.01.T01:intron 40.0%
CCAATCACTATGGTATCAAG+AGG + Chr1:8928654-8928673 MsG0180000632.01.T01:CDS 40.0%
CCTCTTGATACCATAGTGAT+TGG - Chr1:8928657-8928676 None:intergenic 40.0%
TTGGGAAACACCAATCACTA+TGG + Chr1:8928644-8928663 MsG0180000632.01.T01:CDS 40.0%
! ACTTCCTCCGTTCCTTTTAA+TGG + Chr1:8928016-8928035 MsG0180000632.01.T01:intron 40.0%
! TTGGTTGTGGAATTCTGGAA+TGG + Chr1:8927667-8927686 MsG0180000632.01.T01:intron 40.0%
!! AAGGTTTTGGTCATTGGTTG+TGG + Chr1:8927654-8927673 MsG0180000632.01.T01:intron 40.0%
ACAAGAGCAATGTGCCTACT+TGG + Chr1:8928968-8928987 MsG0180000632.01.T01:CDS 45.0%
CCAGTAGCAACAATAAGCCA+AGG - Chr1:8927529-8927548 None:intergenic 45.0%
CCTTGGCTTATTGTTGCTAC+TGG + Chr1:8927526-8927545 MsG0180000632.01.T01:CDS 45.0%
GAGTTTCGTGCAACCAAATG+AGG - Chr1:8927728-8927747 None:intergenic 45.0%
GGTATCAAGAGGCCTAAAAC+TGG + Chr1:8928665-8928684 MsG0180000632.01.T01:CDS 45.0%
GTGACCATTAAAAGGAACGG+AGG - Chr1:8928023-8928042 None:intergenic 45.0%
! GGTCATTGGTTGTGGAATTC+TGG + Chr1:8927662-8927681 MsG0180000632.01.T01:intron 45.0%
!!! TTATATTAATACTTTTAATA+AGG + Chr1:8928322-8928341 MsG0180000632.01.T01:intron 5.0%
CAATGTGCCTACTTGGCTCA+AGG + Chr1:8928975-8928994 MsG0180000632.01.T01:CDS 50.0%
Chromosome Type Strat End Strand Name
Chr1 gene 8927397 8929008 8927397 ID=MsG0180000632.01;Name=MsG0180000632.01
Chr1 mRNA 8927397 8929008 8927397 ID=MsG0180000632.01.T01;Parent=MsG0180000632.01;Name=MsG0180000632.01.T01;_AED=0.35;_eAED=0.35;_QI=0|0|0|1|1|1|3|0|214
Chr1 exon 8927397 8927669 8927397 ID=MsG0180000632.01.T01:exon:2150;Parent=MsG0180000632.01.T01
Chr1 exon 8928524 8928761 8928524 ID=MsG0180000632.01.T01:exon:2149;Parent=MsG0180000632.01.T01
Chr1 exon 8928875 8929008 8928875 ID=MsG0180000632.01.T01:exon:2148;Parent=MsG0180000632.01.T01
Chr1 CDS 8927397 8927669 8927397 ID=MsG0180000632.01.T01:cds;Parent=MsG0180000632.01.T01
Chr1 CDS 8928524 8928761 8928524 ID=MsG0180000632.01.T01:cds;Parent=MsG0180000632.01.T01
Chr1 CDS 8928875 8929008 8928875 ID=MsG0180000632.01.T01:cds;Parent=MsG0180000632.01.T01
Gene Sequence

>MsG0180000632.01.T01

ATGGAATCCTATGCTGATCACTTTGATATTCATCCTAGGTTTAATCAAACTGTTCTTAGTGCTGAGTTTGATTCAAGTTCTCAAATTTGGATGGTTAGAACTAAAGAAGCTGATTTTCAGTATTTTTCACCTTGGCTTATTGTTGCTACTGGTGAAAATGCTGAACCTGTTTTTCCAACAATTCATGGTATGGAACATTTTCATGGTCCTGTTGCTCATACAAGTGATTATAAATCTGGTTCTGAGTACAAAAATAAAAGGTTTTGGTCATTGGTACATATTCTTCCAAGAGATATGTTTGGTTTCTCAACATATGGAATAGCTATGGGATTATATAAATGGCTTCCATTAAAATTAGTAGACAAGTTTCTCTTGTTAGTTTCAAGCTTCTTCTTGGGAAACACCAATCACTATGGTATCAAGAGGCCTAAAACTGGTCCAATAGAACTCAAACTTGCAACAGGAAAAACTCCTGTACTTGATGTTGGTCAAATAGCACAAATTAAATCCGGTGATGGAAGGTGTGAAAGAGATAACAAGAAATGGTGCAAAATTTTTGATGGACAAGAAAAGGAATTTGATGCAATAATCTTAGCAACAGGGTACAAGAGCAATGTGCCTACTTGGCTCAAGGTTAAAAATTGA

Protein sequence

>MsG0180000632.01.T01

MESYADHFDIHPRFNQTVLSAEFDSSSQIWMVRTKEADFQYFSPWLIVATGENAEPVFPTIHGMEHFHGPVAHTSDYKSGSEYKNKRFWSLVHILPRDMFGFSTYGIAMGLYKWLPLKLVDKFLLLVSSFFLGNTNHYGIKRPKTGPIELKLATGKTPVLDVGQIAQIKSGDGRCERDNKKWCKIFDGQEKEFDAIILATGYKSNVPTWLKVKN*