AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar ZhongmuNo.1 / MsG0280010316.01

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MsG0280010316.01.T01 MTR_2g081810 83.333 186 11 2 1 186 1 166 5.10e-105 303
MsG0280010316.01.T01 MTR_8g466030 75.806 186 25 2 1 186 1 166 1.20e-95 279
MsG0280010316.01.T01 MTR_4g091900 70.109 184 35 2 3 186 4 167 7.76e-87 256
MsG0280010316.01.T01 MTR_2g011080 64.516 186 46 2 1 186 1 166 6.74e-81 241
MsG0280010316.01.T01 MTR_4g091900 69.714 175 33 2 12 186 1 155 7.47e-81 241
MsG0280010316.01.T01 MTR_2g011080 64.045 178 44 2 9 186 4 161 3.62e-76 229
MsG0280010316.01.T01 MTR_5g070030 56.757 185 59 3 3 186 4 168 2.96e-68 209
MsG0280010316.01.T01 MTR_4g069250 55.676 185 61 3 3 186 51 215 4.59e-62 195
MsG0280010316.01.T01 MTR_5g019620 52.973 185 66 3 3 186 52 216 6.18e-60 190
MsG0280010316.01.T01 MTR_2g011080 58.779 131 38 1 56 186 1 115 4.21e-51 164
MsG0280010316.01.T01 MTR_8g466080 64.948 97 18 1 89 185 1 81 3.81e-36 124
MsG0280010316.01.T01 MTR_4g091890 59.596 99 20 2 88 186 1 79 5.56e-32 114
MsG0280010316.01.T01 MTR_8g466110 43.511 131 32 2 56 186 1 89 6.24e-25 95.1
MsG0280010316.01.T01 MTR_8g466150 43.511 131 32 2 56 186 1 89 6.24e-25 95.1
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MsG0280010316.01.T01 AT5G54500 75.269 186 26 2 1 186 1 166 7.93e-96 280
MsG0280010316.01.T01 AT4G27270 74.194 186 28 2 1 186 1 166 1.18e-94 276
MsG0280010316.01.T01 AT5G54500 69.543 197 40 2 1 197 1 177 6.38e-91 268
MsG0280010316.01.T01 AT4G27270 73.714 175 26 2 12 186 1 155 3.96e-87 257
MsG0280010316.01.T01 AT4G27270 73.714 175 26 2 12 186 1 155 3.96e-87 257
MsG0280010316.01.T01 AT4G36750 57.297 185 58 3 3 186 73 237 1.31e-66 207
MsG0280010316.01.T01 AT5G58800 54.255 188 64 4 1 186 1 168 1.08e-62 196
MsG0280010316.01.T01 AT5G58800 54.255 188 64 4 1 186 1 168 1.08e-62 196
MsG0280010316.01.T01 AT4G27270 72.519 131 20 1 56 186 1 115 1.14e-62 193

Find 64 sgRNAs with CRISPR-Local

Find 197 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
TTTGGCTTCCCAACAAGATT+TGG 0.281962 2:+67867074 MsG0280010316.01.T01:CDS
TTGTATTGTTTGTTCTTGTT+AGG 0.337549 2:+67868033 MsG0280010316.01.T01:three_prime_UTR
AGTCAAATTATGTTCGTATA+AGG 0.343831 2:+67867354 MsG0280010316.01.T01:CDS
AATACTTGCCTGCCATAGTT+TGG 0.355319 2:-67866219 None:intergenic
GCTTTGTATGTGTGTTATAT+AGG 0.358776 2:+67867968 MsG0280010316.01.T01:three_prime_UTR
TGGCAAGATATTTCCCTTGA+TGG 0.369825 2:-67867759 None:intergenic
TGGAAAGCACAATCGCTTGC+AGG 0.372580 2:+67867146 MsG0280010316.01.T01:CDS
ATTGTTTGTTCTTGTTAGGC+TGG 0.390176 2:+67868037 MsG0280010316.01.T01:three_prime_UTR
TGCTGCAATTCAAGCTCACT+TGG 0.403953 2:-67867730 None:intergenic
TTCCTTGAGCTTCTTTGTGA+TGG 0.410437 2:-67867782 None:intergenic
GTTCCAATCGGTTATACATT+CGG 0.413022 2:+67867626 MsG0280010316.01.T01:CDS
AACGGTGCTGTAGAAGATTC+CGG 0.419225 2:-67867177 None:intergenic
GACAGGTACTACTCCATGTA+TGG 0.423761 2:+67866134 MsG0280010316.01.T01:intron
ACCTCCTCTTGCAGAGTCTC+AGG 0.429211 2:-67866969 None:intergenic
ATAGATGTAATACAAAATAT+AGG 0.436938 2:-67868074 None:intergenic
TCACCCTCAGACAGTTCATT+TGG 0.438735 2:-67867041 None:intergenic
GCCATCATTCCAAATCTTGT+TGG 0.445283 2:-67867083 None:intergenic
CCATCATTCCAAATCTTGTT+GGG 0.448149 2:-67867082 None:intergenic
CCCAACAAGATTTGGAATGA+TGG 0.449236 2:+67867082 MsG0280010316.01.T01:CDS
CTTGAGCTTCTTTGTGATGG+TGG 0.452174 2:-67867779 None:intergenic
TAGATGCTACTGGTGGTTTG+TGG 0.475106 2:+67867126 MsG0280010316.01.T01:CDS
GGAATGATATTTGTTCCAAT+CGG 0.481412 2:+67867614 MsG0280010316.01.T01:CDS
CTTGTCCGCCGCCTTGAGAA+CGG 0.492445 2:-67867195 None:intergenic
GTGAAAGGTGGAAGTCCATA+TGG 0.494510 2:+67867674 MsG0280010316.01.T01:CDS
TCGCTTGCAGGCAAGCCTGC+CGG 0.497043 2:+67867158 MsG0280010316.01.T01:CDS
CGTTGAAGCCAAACTATGGC+AGG 0.498411 2:+67866211 MsG0280010316.01.T01:CDS
GGCGTATGTTCCGCACCATA+TGG 0.499254 2:-67867689 None:intergenic
AAGGCGTTGAAGCCAAACTA+TGG 0.501664 2:+67866207 MsG0280010316.01.T01:CDS
CGGAGCCGGCATGTTTGAGA+TGG 0.507130 2:+67867646 MsG0280010316.01.T01:CDS
AAAGAAAGGTGCTGCTTCTG+TGG 0.513238 2:+67866184 MsG0280010316.01.T01:CDS
AATGAGTGGTACATCACTCT+TGG 0.514522 2:-67867015 None:intergenic
TTTCTCCACATGTCCATACA+TGG 0.519645 2:-67866147 None:intergenic
TTGTTTGTTCTTGTTAGGCT+GGG 0.524234 2:+67868038 MsG0280010316.01.T01:three_prime_UTR
GCTTACTGCTATTACTCAGC+TGG 0.527936 2:+67867583 MsG0280010316.01.T01:CDS
CTTCTACAGCACCGTTCTCA+AGG 0.536392 2:+67867184 MsG0280010316.01.T01:CDS
AAAGGTGCTGCTTCTGTGGA+AGG 0.539491 2:+67866188 MsG0280010316.01.T01:CDS
GAGATGGAGCAAGTGAAAGG+TGG 0.540398 2:+67867662 MsG0280010316.01.T01:CDS
ATTACTCAGCTGGTTCATCA+TGG 0.541080 2:+67867593 MsG0280010316.01.T01:CDS
TGCGGAACATACGCCGGCGA+TGG 0.545287 2:+67867697 MsG0280010316.01.T01:CDS
TGCAGCAAGCATTCCATCAA+GGG 0.547652 2:+67867746 MsG0280010316.01.T01:CDS
TTGCAGCAAGCATTCCATCA+AGG 0.547761 2:+67867745 MsG0280010316.01.T01:CDS
ATCGGTTATACATTCGGAGC+CGG 0.548677 2:+67867632 MsG0280010316.01.T01:CDS
ATGAGTGGTACATCACTCTT+GGG 0.565086 2:-67867014 None:intergenic
CTAGCAGAAGAAATAAAGAA+AGG 0.566913 2:+67866170 MsG0280010316.01.T01:CDS
GCTCCGAATGTATAACCGAT+TGG 0.573893 2:-67867629 None:intergenic
TACACCAAATGAACTGTCTG+AGG 0.584673 2:+67867037 MsG0280010316.01.T01:CDS
AGTTCATTTGGTGTAATGAG+TGG 0.610687 2:-67867029 None:intergenic
CTACTCCATGTATGGACATG+TGG 0.614664 2:+67866142 MsG0280010316.01.T01:CDS
ACCTGAGACTCTGCAAGAGG+AGG 0.617531 2:+67866968 MsG0280010316.01.T01:CDS
CAGCACCGTTCTCAAGGCGG+CGG 0.620342 2:+67867190 MsG0280010316.01.T01:CDS
CACCATCACAAAGAAGCTCA+AGG 0.623802 2:+67867780 MsG0280010316.01.T01:CDS
ACTCTGCAAGAGGAGGTGCT+TGG 0.623982 2:+67866975 MsG0280010316.01.T01:CDS
GTGCTGTAGAAGATTCCGGC+AGG 0.625362 2:-67867173 None:intergenic
GTGTGTTATATAGGATGCAG+TGG 0.625654 2:+67867977 MsG0280010316.01.T01:three_prime_UTR
GGTACCTGAGACTCTGCAAG+AGG 0.643789 2:+67866965 MsG0280010316.01.T01:intron
TTGCTCCATCTCAAACATGC+CGG 0.649670 2:-67867651 None:intergenic
AATGAACTGTCTGAGGGTGA+TGG 0.650302 2:+67867044 MsG0280010316.01.T01:CDS
TGGCTGTCTTGAACCATCGC+CGG 0.656734 2:-67867710 None:intergenic
TTTGAGATGGAGCAAGTGAA+AGG 0.659849 2:+67867659 MsG0280010316.01.T01:CDS
AGGTGGAAGTCCATATGGTG+CGG 0.663534 2:+67867679 MsG0280010316.01.T01:CDS
ACACCAAATGAACTGTCTGA+GGG 0.665595 2:+67867038 MsG0280010316.01.T01:CDS
ATATGGTGCGGAACATACGC+CGG 0.668465 2:+67867691 MsG0280010316.01.T01:CDS
AGTGGTACATCACTCTTGGG+TGG 0.722965 2:-67867011 None:intergenic
CTACAGCACCGTTCTCAAGG+CGG 0.736634 2:+67867187 MsG0280010316.01.T01:CDS

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!! AAATATAAATATTGTTATCT+TGG - Chr2:67867540-67867559 None:intergenic 10.0%
!! ATAAAAAAACAAGAATTATT+CGG - Chr2:67866819-67866838 None:intergenic 10.0%
!!! TTTGATTTTTATATTTGATT+TGG + Chr2:67865222-67865241 MsG0280010316.01.T01:intron 10.0%
!! ATAAATTTACTTGAAACATT+TGG - Chr2:67865881-67865900 None:intergenic 15.0%
!! ATAGATGTAATACAAAATAT+AGG - Chr2:67868077-67868096 None:intergenic 15.0%
!!! ATTTGACAGTTAAAAATTTT+GGG - Chr2:67865579-67865598 None:intergenic 15.0%
!!! TATTTGACAGTTAAAAATTT+TGG - Chr2:67865580-67865599 None:intergenic 15.0%
!!! TTATATAATTTGTGAAATGA+AGG + Chr2:67866772-67866791 MsG0280010316.01.T01:intron 15.0%
!! AAATATTGTTATCTTGGTAA+TGG - Chr2:67867534-67867553 None:intergenic 20.0%
!! ACCTTAAATAATTGTGTAAT+TGG - Chr2:67866379-67866398 None:intergenic 20.0%
!! CAAATAAAAGGAAACAAATT+TGG - Chr2:67865206-67865225 None:intergenic 20.0%
!! TAAAAAATGGTAAATGGTAA+GGG - Chr2:67867878-67867897 None:intergenic 20.0%
!! TAAATACTTGTGAAATTGTT+TGG - Chr2:67866681-67866700 None:intergenic 20.0%
!! TCCAATTACACAATTATTTA+AGG + Chr2:67866375-67866394 MsG0280010316.01.T01:intron 20.0%
!!! AAAAGGTTCTATTTTTTCAT+TGG + Chr2:67865053-67865072 MsG0280010316.01.T01:intron 20.0%
!!! AAGATTAGATTTTGTTTACT+GGG + Chr2:67864899-67864918 MsG0280010316.01.T01:intron 20.0%
!!! AAGTTTCAAGTGTATATTTT+TGG + Chr2:67865722-67865741 MsG0280010316.01.T01:intron 20.0%
!!! AGCATTTTCTTTTTGTAAAT+TGG - Chr2:67867951-67867970 None:intergenic 20.0%
!!! ATCGTTTTTCAAAATAAGTA+TGG + Chr2:67865899-67865918 MsG0280010316.01.T01:intron 20.0%
!!! CTATTTTCATAAGTATATCT+TGG + Chr2:67867383-67867402 MsG0280010316.01.T01:intron 20.0%
!!! TATTAAATCTCAAACCTTTT+TGG + Chr2:67864944-67864963 MsG0280010316.01.T01:intron 20.0%
!!! TTATTTGATTTTGTAAGTGA+AGG + Chr2:67866878-67866897 MsG0280010316.01.T01:intron 20.0%
!!! TTTTCTTTTTGTAAATTGGA+AGG - Chr2:67867947-67867966 None:intergenic 20.0%
!!! TTTTTCTATAGAAAGAGTAT+TGG + Chr2:67865008-67865027 MsG0280010316.01.T01:intron 20.0%
!!! TTTTTCTTTTTTTTGTACTG+TGG + Chr2:67864857-67864876 MsG0280010316.01.T01:intron 20.0%
! AAAAAAACAGCAATAACAAC+AGG - Chr2:67864839-67864858 None:intergenic 25.0%
! AACTGTGAAATTCTTCTATT+TGG + Chr2:67865670-67865689 MsG0280010316.01.T01:intron 25.0%
! AAGAATTATTCGGATATCAT+AGG - Chr2:67866809-67866828 None:intergenic 25.0%
! AGTCAAATTATGTTCGTATA+AGG + Chr2:67867354-67867373 MsG0280010316.01.T01:CDS 25.0%
! AGTTAATGAGTAATACTACT+AGG + Chr2:67865933-67865952 MsG0280010316.01.T01:intron 25.0%
! ATTAAGTTCAAAACCAACTA+TGG - Chr2:67866402-67866421 None:intergenic 25.0%
! ATTAAGTTCTTTATCCAAAC+AGG - Chr2:67866503-67866522 None:intergenic 25.0%
! CATATGGAAAAACATTTGTT+GGG - Chr2:67865831-67865850 None:intergenic 25.0%
! CTAAAAAATGGTAAATGGTA+AGG - Chr2:67867879-67867898 None:intergenic 25.0%
! GAAGTCTAAAAAATGGTAAA+TGG - Chr2:67867884-67867903 None:intergenic 25.0%
! TATATGCGAAGTCTAAAAAA+TGG - Chr2:67867891-67867910 None:intergenic 25.0%
! TCTTAAAGCTAAAAGATAAC+AGG - Chr2:67866749-67866768 None:intergenic 25.0%
! TTGTATTGTTTGTTCTTGTT+AGG + Chr2:67868033-67868052 MsG0280010316.01.T01:three_prime_UTR 25.0%
!! AACCTTTTTGGATTTTTCTT+TGG + Chr2:67864956-67864975 MsG0280010316.01.T01:intron 25.0%
!! AATTATTTAAGGTCCATAGT+TGG + Chr2:67866386-67866405 MsG0280010316.01.T01:intron 25.0%
!! TTTGATCTTTTGGTAGAAAA+AGG + Chr2:67865036-67865055 MsG0280010316.01.T01:intron 25.0%
!!! AAATATGTTTGTTGTCAACT+AGG + Chr2:67865595-67865614 MsG0280010316.01.T01:intron 25.0%
!!! AGGGTTTTAAAGCAATATTT+CGG - Chr2:67867859-67867878 None:intergenic 25.0%
!!! ATTGCATTAGACCTTTTTTA+TGG + Chr2:67865964-67865983 MsG0280010316.01.T01:intron 25.0%
!!! ATTGGTTTTCTTTGATCTTT+TGG + Chr2:67865026-67865045 MsG0280010316.01.T01:intron 25.0%
!!! CAACTTTGAAACCATAAAAA+AGG - Chr2:67865978-67865997 None:intergenic 25.0%
!!! CAAGATTAGATTTTGTTTAC+TGG + Chr2:67864898-67864917 MsG0280010316.01.T01:intron 25.0%
!!! GGTTTTGTTTAATTCTCAAA+TGG + Chr2:67864920-67864939 MsG0280010316.01.T01:intron 25.0%
AAAACATTGGAATCCATTAC+AGG + Chr2:67867561-67867580 MsG0280010316.01.T01:intron 30.0%
AATTGAAACCATCCTCTAAT+TGG + Chr2:67865151-67865170 MsG0280010316.01.T01:intron 30.0%
AGCTAAAAGATAACAGGATA+CGG - Chr2:67866743-67866762 None:intergenic 30.0%
ATGAAGAATAGCAATGAGTT+GGG + Chr2:67865457-67865476 MsG0280010316.01.T01:intron 30.0%
CTAGCAGAAGAAATAAAGAA+AGG + Chr2:67866170-67866189 MsG0280010316.01.T01:CDS 30.0%
CTCACAAAGATCACATATTT+GGG + Chr2:67865330-67865349 MsG0280010316.01.T01:intron 30.0%
GACCAAAGAAAAATCCAAAA+AGG - Chr2:67864961-67864980 None:intergenic 30.0%
GCATATGGAAAAACATTTGT+TGG - Chr2:67865832-67865851 None:intergenic 30.0%
GCTTTGTATGTGTGTTATAT+AGG + Chr2:67867968-67867987 MsG0280010316.01.T01:three_prime_UTR 30.0%
GGAATGATATTTGTTCCAAT+CGG + Chr2:67867614-67867633 MsG0280010316.01.T01:CDS 30.0%
GTACACAAAGAGTCTTTAAA+TGG - Chr2:67865287-67865306 None:intergenic 30.0%
TACTTGAAACATTTGGACTA+GGG - Chr2:67865874-67865893 None:intergenic 30.0%
TCAATTTAGTAAGAACTCGA+GGG + Chr2:67865629-67865648 MsG0280010316.01.T01:intron 30.0%
TCGAATAAACCAATCAAAAC+TGG + Chr2:67866715-67866734 MsG0280010316.01.T01:intron 30.0%
TCTCACAAAGATCACATATT+TGG + Chr2:67865329-67865348 MsG0280010316.01.T01:intron 30.0%
TGCAGAAGGAAATGTAAATT+TGG - Chr2:67866252-67866271 None:intergenic 30.0%
TGCTCAATTATATGACACTA+TGG - Chr2:67866277-67866296 None:intergenic 30.0%
TTAACACATTATGTGCTCTA+TGG - Chr2:67866330-67866349 None:intergenic 30.0%
TTACTTGAAACATTTGGACT+AGG - Chr2:67865875-67865894 None:intergenic 30.0%
TTCAATTTAGTAAGAACTCG+AGG + Chr2:67865628-67865647 MsG0280010316.01.T01:intron 30.0%
! AAGCTCTCCATAATAGTTTT+TGG - Chr2:67866627-67866646 None:intergenic 30.0%
! CATCAAGAAAGTGTGAATAT+TGG + Chr2:67865408-67865427 MsG0280010316.01.T01:intron 30.0%
! GCTGTTTCCAAAAACTATTA+TGG + Chr2:67866617-67866636 MsG0280010316.01.T01:intron 30.0%
! TCAAATAAGTCGATTCAAAC+TGG + Chr2:67867493-67867512 MsG0280010316.01.T01:intron 30.0%
! TTTTTAGACACAGCATTTTG+TGG - Chr2:67867468-67867487 None:intergenic 30.0%
!! CTCATTATTCTGGTCTTATT+TGG + Chr2:67867242-67867261 MsG0280010316.01.T01:intron 30.0%
!!! AGGAATTTTTTTGCAGAAAC+AGG - Chr2:67867927-67867946 None:intergenic 30.0%
!!! ATTTGGAAGTGTGTTTTTTG+TGG - Chr2:67865189-67865208 None:intergenic 30.0%
!!! GTTTTTTATGGTGTTTTGAC+AGG + Chr2:67866117-67866136 MsG0280010316.01.T01:intron 30.0%
AACAAGCCAAACACATCAAA+AGG - Chr2:67865112-67865131 None:intergenic 35.0%
AAGTAACTGAGATCCATTTG+AGG + Chr2:67865794-67865813 MsG0280010316.01.T01:intron 35.0%
ACAAGCCAAACACATCAAAA+GGG - Chr2:67865111-67865130 None:intergenic 35.0%
AGAATTGTGCAAAATTGTCC+AGG - Chr2:67866052-67866071 None:intergenic 35.0%
AGTAACTGAGATCCATTTGA+GGG + Chr2:67865795-67865814 MsG0280010316.01.T01:intron 35.0%
ATTGTTTGTTCTTGTTAGGC+TGG + Chr2:67868037-67868056 MsG0280010316.01.T01:three_prime_UTR 35.0%
CCATCATTCCAAATCTTGTT+GGG - Chr2:67867085-67867104 None:intergenic 35.0%
CTAAAAGATAACAGGATACG+GGG - Chr2:67866741-67866760 None:intergenic 35.0%
GATCATAATCATTTCTCTGC+TGG + Chr2:67866004-67866023 MsG0280010316.01.T01:intron 35.0%
GATGAAGAATAGCAATGAGT+TGG + Chr2:67865456-67865475 MsG0280010316.01.T01:intron 35.0%
GCTAAAAGATAACAGGATAC+GGG - Chr2:67866742-67866761 None:intergenic 35.0%
GTTCCAATCGGTTATACATT+CGG + Chr2:67867626-67867645 MsG0280010316.01.T01:CDS 35.0%
TAATAGCAGTAAGCCTGTAA+TGG - Chr2:67867577-67867596 None:intergenic 35.0%
TGAAGAATAGCAATGAGTTG+GGG + Chr2:67865458-67865477 MsG0280010316.01.T01:intron 35.0%
TTGTTTGTTCTTGTTAGGCT+GGG + Chr2:67868038-67868057 MsG0280010316.01.T01:three_prime_UTR 35.0%
! AAAGCTTTTCTAGATGCTAC+TGG + Chr2:67867116-67867135 MsG0280010316.01.T01:CDS 35.0%
! AGAATCCCTTTTGATGTGTT+TGG + Chr2:67865103-67865122 MsG0280010316.01.T01:intron 35.0%
! CCTTTTATGAACCAAGCAAT+CGG - Chr2:67865506-67865525 None:intergenic 35.0%
! CTTTTATGAACCAAGCAATC+GGG - Chr2:67865505-67865524 None:intergenic 35.0%
! GATCTTATCTATTTAGCTCC+TGG + Chr2:67866031-67866050 MsG0280010316.01.T01:intron 35.0%
!! AGTTCATTTGGTGTAATGAG+TGG - Chr2:67867032-67867051 None:intergenic 35.0%
!! TGGCTGGTTAAATGTTTTGT+TGG + Chr2:67866451-67866470 MsG0280010316.01.T01:intron 35.0%
!! TGGTAATGGAGTAAGAAACA+AGG - Chr2:67867520-67867539 None:intergenic 35.0%
!!! AAGTTCCTTTGTTTTGACCA+TGG + Chr2:67866476-67866495 MsG0280010316.01.T01:intron 35.0%
!!! GGCTTGTTATGCATTTTGTT+TGG + Chr2:67865124-67865143 MsG0280010316.01.T01:intron 35.0%
AATACTTGCCTGCCATAGTT+TGG - Chr2:67866222-67866241 None:intergenic 40.0%
AATGAGTGGTACATCACTCT+TGG - Chr2:67867018-67867037 None:intergenic 40.0%
AATGGATCTCAGTTACTTCG+AGG - Chr2:67865792-67865811 None:intergenic 40.0%
ACACCAAATGAACTGTCTGA+GGG + Chr2:67867038-67867057 MsG0280010316.01.T01:CDS 40.0%
ACTGTACTTAATCTGTGAGC+AGG + Chr2:67866944-67866963 MsG0280010316.01.T01:intron 40.0%
ATGACACTATGGAATGCAGA+AGG - Chr2:67866266-67866285 None:intergenic 40.0%
ATGAGTGGTACATCACTCTT+GGG - Chr2:67867017-67867036 None:intergenic 40.0%
ATGGATCTCAGTTACTTCGA+GGG - Chr2:67865791-67865810 None:intergenic 40.0%
CACTTGAAACTTGAAAGCAG+TGG - Chr2:67865714-67865733 None:intergenic 40.0%
CATGCCATTCAGCTGTTATA+TGG + Chr2:67866431-67866450 MsG0280010316.01.T01:intron 40.0%
CCGATTGCTTGGTTCATAAA+AGG + Chr2:67865503-67865522 MsG0280010316.01.T01:intron 40.0%
GCACATCTGTCTCATTATTC+TGG + Chr2:67867232-67867251 MsG0280010316.01.T01:intron 40.0%
GCCATCATTCCAAATCTTGT+TGG - Chr2:67867086-67867105 None:intergenic 40.0%
GTAACTGAGATCCATTTGAG+GGG + Chr2:67865796-67865815 MsG0280010316.01.T01:intron 40.0%
GTGTGTTATATAGGATGCAG+TGG + Chr2:67867977-67867996 MsG0280010316.01.T01:three_prime_UTR 40.0%
TACACCAAATGAACTGTCTG+AGG + Chr2:67867037-67867056 MsG0280010316.01.T01:CDS 40.0%
TCTTTATCCAAACAGGACCA+TGG - Chr2:67866496-67866515 None:intergenic 40.0%
TGGCAAGATATTTCCCTTGA+TGG - Chr2:67867762-67867781 None:intergenic 40.0%
TTCCTTGAGCTTCTTTGTGA+TGG - Chr2:67867785-67867804 None:intergenic 40.0%
TTTCTCCACATGTCCATACA+TGG - Chr2:67866150-67866169 None:intergenic 40.0%
! ATTACTCAGCTGGTTCATCA+TGG + Chr2:67867593-67867612 MsG0280010316.01.T01:CDS 40.0%
! CCCAACAAGATTTGGAATGA+TGG + Chr2:67867082-67867101 MsG0280010316.01.T01:CDS 40.0%
! GGAAAAACATTTGTTGGGAG+AGG - Chr2:67865826-67865845 None:intergenic 40.0%
! TTTGAGATGGAGCAAGTGAA+AGG + Chr2:67867659-67867678 MsG0280010316.01.T01:CDS 40.0%
! TTTGGCTTCCCAACAAGATT+TGG + Chr2:67867074-67867093 MsG0280010316.01.T01:CDS 40.0%
!!! AAGTGTGTTTTTTGTGGCAG+AGG - Chr2:67865183-67865202 None:intergenic 40.0%
AATGAACTGTCTGAGGGTGA+TGG + Chr2:67867044-67867063 MsG0280010316.01.T01:CDS 45.0%
AGTTCTACAGTTGTGCACGA+AGG - Chr2:67865762-67865781 None:intergenic 45.0%
ATCGGTTATACATTCGGAGC+CGG + Chr2:67867632-67867651 MsG0280010316.01.T01:CDS 45.0%
CACCATCACAAAGAAGCTCA+AGG + Chr2:67867780-67867799 MsG0280010316.01.T01:CDS 45.0%
CAGAGGATGGTTCCAATTAG+AGG - Chr2:67865166-67865185 None:intergenic 45.0%
CAGGACCATGGTCAAAACAA+AGG - Chr2:67866484-67866503 None:intergenic 45.0%
CCAGCCATATAACAGCTGAA+TGG - Chr2:67866438-67866457 None:intergenic 45.0%
CCATTCAGCTGTTATATGGC+TGG + Chr2:67866435-67866454 MsG0280010316.01.T01:intron 45.0%
CTACTCCATGTATGGACATG+TGG + Chr2:67866142-67866161 MsG0280010316.01.T01:CDS 45.0%
CTTGAGCTTCTTTGTGATGG+TGG - Chr2:67867782-67867801 None:intergenic 45.0%
GACAGGTACTACTCCATGTA+TGG + Chr2:67866134-67866153 MsG0280010316.01.T01:intron 45.0%
GCTCCGAATGTATAACCGAT+TGG - Chr2:67867632-67867651 None:intergenic 45.0%
GCTTACTGCTATTACTCAGC+TGG + Chr2:67867583-67867602 MsG0280010316.01.T01:CDS 45.0%
GGATGGTTCCAATTAGAGGA+TGG - Chr2:67865162-67865181 None:intergenic 45.0%
GTGAAAGGTGGAAGTCCATA+TGG + Chr2:67867674-67867693 MsG0280010316.01.T01:CDS 45.0%
TAGATGCTACTGGTGGTTTG+TGG + Chr2:67867126-67867145 MsG0280010316.01.T01:CDS 45.0%
TCACCCTCAGACAGTTCATT+TGG - Chr2:67867044-67867063 None:intergenic 45.0%
TGCAGCAAGCATTCCATCAA+GGG + Chr2:67867746-67867765 MsG0280010316.01.T01:CDS 45.0%
TGCTGCAATTCAAGCTCACT+TGG - Chr2:67867733-67867752 None:intergenic 45.0%
TGGATCTCAGTTACTTCGAG+GGG - Chr2:67865790-67865809 None:intergenic 45.0%
TTGCAGCAAGCATTCCATCA+AGG + Chr2:67867745-67867764 MsG0280010316.01.T01:CDS 45.0%
TTGCTCCATCTCAAACATGC+CGG - Chr2:67867654-67867673 None:intergenic 45.0%
TTGTGCACGAAGGATAGCTT+TGG - Chr2:67865752-67865771 None:intergenic 45.0%
! GCTTTTCTAGATGCTACTGG+TGG + Chr2:67867119-67867138 MsG0280010316.01.T01:CDS 45.0%
! TTCCATATGCGCACTGATCA+GGG + Chr2:67865842-67865861 MsG0280010316.01.T01:intron 45.0%
! TTTCCATATGCGCACTGATC+AGG + Chr2:67865841-67865860 MsG0280010316.01.T01:intron 45.0%
!! AAAGAAAGGTGCTGCTTCTG+TGG + Chr2:67866184-67866203 MsG0280010316.01.T01:CDS 45.0%
!! AACGGTGCTGTAGAAGATTC+CGG - Chr2:67867180-67867199 None:intergenic 45.0%
!! AAGGCGTTGAAGCCAAACTA+TGG + Chr2:67866207-67866226 MsG0280010316.01.T01:CDS 45.0%
!! CACAGCATTTTGTGGACTGT+TGG - Chr2:67867460-67867479 None:intergenic 45.0%
!! GTTTTGACCATGGTCCTGTT+TGG + Chr2:67866486-67866505 MsG0280010316.01.T01:intron 45.0%
!!! GAGGGTGATGGTTTTGTCTT+TGG + Chr2:67867056-67867075 MsG0280010316.01.T01:CDS 45.0%
!!! GTGTTTTTTGTGGCAGAGGA+TGG - Chr2:67865179-67865198 None:intergenic 45.0%
!! AAATATAAAAATCAAATAAA+AGG - Chr2:67865218-67865237 None:intergenic 5.0%
!!! ATATTTATATTTTAAAACAT+TGG + Chr2:67867548-67867567 MsG0280010316.01.T01:intron 5.0%
!!! TTAATTAAAAATGTTTTTTA+TGG + Chr2:67866105-67866124 MsG0280010316.01.T01:intron 5.0%
AGGTGGAAGTCCATATGGTG+CGG + Chr2:67867679-67867698 MsG0280010316.01.T01:CDS 50.0%
AGTGGTACATCACTCTTGGG+TGG - Chr2:67867014-67867033 None:intergenic 50.0%
ATATGGTGCGGAACATACGC+CGG + Chr2:67867691-67867710 MsG0280010316.01.T01:CDS 50.0%
ATCCCTGATCAGTGCGCATA+TGG - Chr2:67865847-67865866 None:intergenic 50.0%
CAGCATCAGTTCCTGAGCTA+AGG + Chr2:67866295-67866314 MsG0280010316.01.T01:intron 50.0%
CGTTGAAGCCAAACTATGGC+AGG + Chr2:67866211-67866230 MsG0280010316.01.T01:CDS 50.0%
CTTCTACAGCACCGTTCTCA+AGG + Chr2:67867184-67867203 MsG0280010316.01.T01:CDS 50.0%
GAGATGGAGCAAGTGAAAGG+TGG + Chr2:67867662-67867681 MsG0280010316.01.T01:CDS 50.0%
GGACAAATCAGCCTTAGCTC+AGG - Chr2:67866309-67866328 None:intergenic 50.0%
TTAGTAAGAACTCGAGGGCG+AGG + Chr2:67865634-67865653 MsG0280010316.01.T01:intron 50.0%
! TGGAAAGCACAATCGCTTGC+AGG + Chr2:67867146-67867165 MsG0280010316.01.T01:CDS 50.0%
!! AAAGGTGCTGCTTCTGTGGA+AGG + Chr2:67866188-67866207 MsG0280010316.01.T01:CDS 50.0%
!! GATACGGGGCCAGTTTTGAT+TGG - Chr2:67866727-67866746 None:intergenic 50.0%
ACCTCCTCTTGCAGAGTCTC+AGG - Chr2:67866972-67866991 None:intergenic 55.0%
ACCTGAGACTCTGCAAGAGG+AGG + Chr2:67866968-67866987 MsG0280010316.01.T01:CDS 55.0%
CTACAGCACCGTTCTCAAGG+CGG + Chr2:67867187-67867206 MsG0280010316.01.T01:CDS 55.0%
GAACCAAGCAATCGGGACGA+GGG - Chr2:67865498-67865517 None:intergenic 55.0%
GGAGAGGTCAACCCCTCAAA+TGG - Chr2:67865810-67865829 None:intergenic 55.0%
GGCGTATGTTCCGCACCATA+TGG - Chr2:67867692-67867711 None:intergenic 55.0%
GGTACCTGAGACTCTGCAAG+AGG + Chr2:67866965-67866984 MsG0280010316.01.T01:intron 55.0%
GTGCTGTAGAAGATTCCGGC+AGG - Chr2:67867176-67867195 None:intergenic 55.0%
TGAACCAAGCAATCGGGACG+AGG - Chr2:67865499-67865518 None:intergenic 55.0%
TGGCTGTCTTGAACCATCGC+CGG - Chr2:67867713-67867732 None:intergenic 55.0%
!! ACTCTGCAAGAGGAGGTGCT+TGG + Chr2:67866975-67866994 MsG0280010316.01.T01:CDS 55.0%
!! CGGGACGAGGGCATTAACAT+CGG - Chr2:67865486-67865505 None:intergenic 55.0%
ATGCCCTCGTCCCGATTGCT+TGG + Chr2:67865492-67865511 MsG0280010316.01.T01:intron 60.0%
CGAGGGCGAGGTGGTAGAAA+AGG + Chr2:67865646-67865665 MsG0280010316.01.T01:intron 60.0%
CGGAGCCGGCATGTTTGAGA+TGG + Chr2:67867646-67867665 MsG0280010316.01.T01:CDS 60.0%
CTTGTCCGCCGCCTTGAGAA+CGG - Chr2:67867198-67867217 None:intergenic 60.0%
GTAAGAACTCGAGGGCGAGG+TGG + Chr2:67865637-67865656 MsG0280010316.01.T01:intron 60.0%
CAGCACCGTTCTCAAGGCGG+CGG + Chr2:67867190-67867209 MsG0280010316.01.T01:CDS 65.0%
TCGCTTGCAGGCAAGCCTGC+CGG + Chr2:67867158-67867177 MsG0280010316.01.T01:CDS 65.0%
TGCGGAACATACGCCGGCGA+TGG + Chr2:67867697-67867716 MsG0280010316.01.T01:CDS 65.0%
Chromosome Type Strat End Strand Name
Chr2 gene 67864720 67868118 67864720 ID=MsG0280010316.01;Name=MsG0280010316.01
Chr2 mRNA 67864720 67868118 67864720 ID=MsG0280010316.01.T01;Parent=MsG0280010316.01;Name=MsG0280010316.01.T01;_AED=0.36;_eAED=0.40;_QI=0|0|0.2|1|0.75|0.8|5|220|251
Chr2 exon 67864720 67864742 67864720 ID=MsG0280010316.01.T01:exon:18435;Parent=MsG0280010316.01.T01
Chr2 exon 67866139 67866244 67866139 ID=MsG0280010316.01.T01:exon:18436;Parent=MsG0280010316.01.T01
Chr2 exon 67866966 67867227 67866966 ID=MsG0280010316.01.T01:exon:18437;Parent=MsG0280010316.01.T01
Chr2 exon 67867327 67867375 67867327 ID=MsG0280010316.01.T01:exon:18438;Parent=MsG0280010316.01.T01
Chr2 exon 67867583 67868118 67867583 ID=MsG0280010316.01.T01:exon:18439;Parent=MsG0280010316.01.T01
Chr2 CDS 67864720 67864742 67864720 ID=MsG0280010316.01.T01:cds;Parent=MsG0280010316.01.T01
Chr2 CDS 67866139 67866244 67866139 ID=MsG0280010316.01.T01:cds;Parent=MsG0280010316.01.T01
Chr2 CDS 67866966 67867227 67866966 ID=MsG0280010316.01.T01:cds;Parent=MsG0280010316.01.T01
Chr2 CDS 67867327 67867375 67867327 ID=MsG0280010316.01.T01:cds;Parent=MsG0280010316.01.T01
Chr2 CDS 67867583 67867898 67867583 ID=MsG0280010316.01.T01:cds;Parent=MsG0280010316.01.T01
Chr2 three_prime_UTR 67867899 67868118 67867899 ID=MsG0280010316.01.T01:three_prime_utr;Parent=MsG0280010316.01.T01
Gene Sequence

>MsG0280010316.01.T01

ATGGCTGTCAAACTTTACATCGTGTACTACTCCATGTATGGACATGTGGAGAAACTAGCAGAAGAAATAAAGAAAGGTGCTGCTTCTGTGGAAGGCGTTGAAGCCAAACTATGGCAGGCAAGTATTTGTGTACCTGAGACTCTGCAAGAGGAGGTGCTTGGTAAGATGAGTGCACCACCCAAGAGTGATGTACCACTCATTACACCAAATGAACTGTCTGAGGGTGATGGTTTTGTCTTTGGCTTCCCAACAAGATTTGGAATGATGGCTGCTCAATTCAAAGCTTTTCTAGATGCTACTGGTGGTTTGTGGAAAGCACAATCGCTTGCAGGCAAGCCTGCCGGAATCTTCTACAGCACCGTTCTCAAGGCGGCGGACAAGAGACTACAGCTTGTTTTTATAAGAAAAGATAAAATAAAGTCAAATTATGTTCGTATAAGGCTTACTGCTATTACTCAGCTGGTTCATCATGGAATGATATTTGTTCCAATCGGTTATACATTCGGAGCCGGCATGTTTGAGATGGAGCAAGTGAAAGGTGGAAGTCCATATGGTGCGGAACATACGCCGGCGATGGTTCAAGACAGCCAAGTGAGCTTGAATTGCAGCAAGCATTCCATCAAGGGAAATATCTTGCCACCATCACAAAGAAGCTCAAGGAAGCTGCATAATGTTGATTTCATATATAGACATATACTATATATTAAACTACACCGAAATATTGCTTTAAAACCCTTACCATTTACCATTTTTTAG
Protein sequence

>MsG0280010316.01.T01

MAVKLYIVYYSMYGHVEKLAEEIKKGAASVEGVEAKLWQASICVPETLQEEVLGKMSAPPKSDVPLITPNELSEGDGFVFGFPTRFGMMAAQFKAFLDATGGLWKAQSLAGKPAGIFYSTVLKAADKRLQLVFIRKDKIKSNYVRIRLTAITQLVHHGMIFVPIGYTFGAGMFEMEQVKGGSPYGAEHTPAMVQDSQVSLNCSKHSIKGNILPPSQRSSRKLHNVDFIYRHILYIKLHRNIALKPLPFTIF*