AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar ZhongmuNo.1 / MsG0280010464.01

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MsG0280010464.01.T01 MTR_2g084585 90.071 141 12 2 1 139 1 141 2.64e-84 251
MsG0280010464.01.T01 MTR_4g036375 54.412 136 59 2 7 139 6 141 4.60e-44 143
MsG0280010464.01.T01 MTR_4g056280 55.303 132 58 1 9 139 10 141 1.15e-42 144
MsG0280010464.01.T01 MTR_8g014910 55.303 132 54 2 10 136 9 140 8.37e-40 137
MsG0280010464.01.T01 MTR_4g056160 53.435 131 59 2 8 137 7 136 2.20e-38 133
MsG0280010464.01.T01 MTR_2g100620 58.036 112 46 1 26 136 28 139 1.51e-37 131
MsG0280010464.01.T01 MTR_6g049220 61.957 92 35 0 16 107 17 108 1.23e-34 119
MsG0280010464.01.T01 MTR_1g025780 43.066 137 71 2 8 137 14 150 1.26e-30 113
MsG0280010464.01.T01 MTR_1g025700 43.448 145 71 3 4 137 15 159 1.75e-28 107
MsG0280010464.01.T01 MTR_3g114120 47.748 111 57 1 27 136 45 155 5.68e-28 106
MsG0280010464.01.T01 MTR_1g025680 42.254 142 74 3 3 136 2 143 2.96e-27 105
MsG0280010464.01.T01 MTR_5g084790 41.732 127 73 1 12 137 15 141 4.53e-27 103
MsG0280010464.01.T01 MTR_8g075230 45.045 111 60 1 27 136 34 144 2.12e-26 101
MsG0280010464.01.T01 MTR_1g025720 41.912 136 70 3 8 136 12 145 2.28e-26 101
MsG0280010464.01.T01 MTR_1g025760 41.429 140 74 3 6 137 50 189 6.41e-26 101
MsG0280010464.01.T01 MTR_4g087985 39.850 133 78 2 8 139 16 147 2.70e-25 99.0
MsG0280010464.01.T01 MTR_3g114090 45.045 111 59 2 28 136 35 145 7.73e-25 97.8
MsG0280010464.01.T01 MTR_3g082830 45.045 111 60 1 27 136 36 146 1.10e-24 97.4
MsG0280010464.01.T01 MTR_5g084750 38.889 126 76 1 12 136 13 138 5.43e-24 95.1
MsG0280010464.01.T01 MTR_2g015080 38.971 136 79 3 8 139 17 152 5.49e-24 95.5
MsG0280010464.01.T01 MTR_8g075200 45.946 111 59 1 28 137 40 150 6.10e-24 95.9
MsG0280010464.01.T01 MTR_8g075220 43.885 139 66 4 11 137 15 153 1.17e-23 94.7
MsG0280010464.01.T01 MTR_3g464730 41.221 131 72 2 8 137 11 137 2.24e-23 93.6
MsG0280010464.01.T01 MTR_3g021460 43.796 137 60 5 8 135 8 136 1.56e-22 91.3
MsG0280010464.01.T01 MTR_5g093090 42.336 137 68 6 10 137 10 144 3.05e-22 88.2
MsG0280010464.01.T01 MTR_5g062890 58.667 75 30 1 9 82 8 82 4.42e-22 84.7
MsG0280010464.01.T01 MTR_5g084770 40.541 111 65 1 27 136 26 136 9.65e-22 89.4
MsG0280010464.01.T01 MTR_5g074270 43.636 110 61 1 29 137 33 142 1.44e-21 89.0
MsG0280010464.01.T01 MTR_5g093090 42.336 137 68 6 10 137 10 144 1.81e-21 87.8
MsG0280010464.01.T01 MTR_5g093090 42.029 138 69 6 9 137 9 144 3.24e-21 87.8
MsG0280010464.01.T01 MTR_8g087870 39.007 141 77 3 1 137 1 136 1.13e-20 86.3
MsG0280010464.01.T01 MTR_5g022640 41.739 115 65 2 27 139 41 155 1.46e-20 86.3
MsG0280010464.01.T01 MTR_5g083040 39.823 113 67 1 27 138 30 142 5.27e-20 84.7
MsG0280010464.01.T01 MTR_5g022630 43.363 113 61 3 27 136 41 153 5.46e-20 84.7
MsG0280010464.01.T01 MTR_0565s0010 36.232 138 83 3 1 136 4 138 7.90e-20 84.0
MsG0280010464.01.T01 MTR_2g017915 38.028 142 79 4 1 136 1 139 8.18e-20 84.0
MsG0280010464.01.T01 MTR_2g017925 38.971 136 68 4 11 136 9 139 2.19e-19 82.8
MsG0280010464.01.T01 MTR_3g052480 39.286 140 68 7 10 135 11 147 2.25e-19 82.8
MsG0280010464.01.T01 MTR_3g052580 39.860 143 70 8 3 135 4 140 5.63e-19 82.0
MsG0280010464.01.T01 MTR_2g461580 42.857 119 53 4 24 135 26 136 5.75e-19 81.6
MsG0280010464.01.T01 MTR_3g052580 39.860 143 70 8 3 135 5 141 5.83e-19 82.0
MsG0280010464.01.T01 MTR_2g017955 40.278 144 73 5 1 136 1 139 7.84e-19 81.6
MsG0280010464.01.T01 MTR_3g052490 48.958 96 44 4 7 99 9 102 1.34e-18 80.9
MsG0280010464.01.T01 MTR_3g024510 40.678 118 65 3 21 136 30 144 2.69e-18 80.1
MsG0280010464.01.T01 MTR_1g025910 41.905 105 59 2 34 136 41 145 4.11e-18 79.3
MsG0280010464.01.T01 MTR_8g087890 38.060 134 70 4 11 137 10 137 7.17e-18 78.6
MsG0280010464.01.T01 MTR_1g079530 37.500 136 78 3 7 136 3 137 7.75e-18 78.6
MsG0280010464.01.T01 MTR_4g035353 40.876 137 74 5 4 136 5 138 3.62e-17 76.6
MsG0280010464.01.T01 MTR_2g017875 48.750 80 39 1 27 106 32 109 8.10e-17 75.9
MsG0280010464.01.T01 MTR_1g079590 43.617 94 51 2 14 107 17 108 1.08e-16 73.6
MsG0280010464.01.T01 MTR_3g005650 40.000 130 69 6 11 137 13 136 1.16e-16 75.5
MsG0280010464.01.T01 MTR_5g078210 36.752 117 62 4 31 140 27 138 2.61e-16 73.9
MsG0280010464.01.T01 MTR_3g098270 39.344 122 61 5 25 138 30 146 2.96e-16 74.3
MsG0280010464.01.T01 MTR_7g116520 34.559 136 86 2 3 136 8 142 3.73e-16 73.9
MsG0280010464.01.T01 MTR_5g078210 36.752 117 62 4 31 140 27 138 4.76e-16 73.6
MsG0280010464.01.T01 MTR_1g079540 44.554 101 54 2 7 107 10 108 5.45e-16 73.6
MsG0280010464.01.T01 MTR_2g087292 41.748 103 52 3 7 102 4 105 5.53e-16 72.8
MsG0280010464.01.T01 MTR_3g005660 40.708 113 57 4 1 106 1 110 7.06e-16 73.2
MsG0280010464.01.T01 MTR_7g116500 35.938 128 79 2 11 136 15 141 7.61e-16 73.2
MsG0280010464.01.T01 MTR_7g116570 32.857 140 81 4 7 136 6 142 7.87e-16 73.2
MsG0280010464.01.T01 MTR_1g069175 41.000 100 56 1 8 107 15 111 1.00e-15 72.8
MsG0280010464.01.T01 MTR_8g074580 32.192 146 79 3 7 135 15 157 1.14e-15 72.8
MsG0280010464.01.T01 MTR_8g079050 45.133 113 55 4 1 106 4 116 1.81e-15 72.0
MsG0280010464.01.T01 MTR_2g017965 36.364 143 81 5 1 136 1 140 2.00e-15 72.0
MsG0280010464.01.T01 MTR_0009s0250 40.816 98 57 1 5 102 9 105 2.33e-15 71.6
MsG0280010464.01.T01 MTR_1g024890 44.681 94 50 2 14 107 18 109 3.18e-15 71.2
MsG0280010464.01.T01 MTR_5g009100 34.862 109 68 2 31 137 48 155 3.82e-15 71.2
MsG0280010464.01.T01 MTR_2g033920 35.897 117 66 5 27 139 33 144 7.31e-15 70.1
MsG0280010464.01.T01 MTR_2g033930 35.036 137 73 5 12 139 12 141 7.89e-15 70.1
MsG0280010464.01.T01 MTR_2g033920 35.897 117 66 5 27 139 33 144 8.43e-15 70.1
MsG0280010464.01.T01 MTR_3g023220 50.000 72 35 1 20 91 24 94 9.16e-15 70.1
MsG0280010464.01.T01 MTR_8g074570 45.556 90 45 2 9 98 22 107 1.61e-14 69.3
MsG0280010464.01.T01 MTR_4g019880 34.375 128 81 2 11 136 14 140 2.43e-14 68.9
MsG0280010464.01.T01 MTR_4g122200 34.559 136 73 6 11 139 18 144 2.74e-14 68.6
MsG0280010464.01.T01 MTR_2g018015 42.241 116 55 4 27 136 34 143 3.58e-14 68.2
MsG0280010464.01.T01 MTR_6g021530 32.847 137 87 4 4 136 3 138 1.87e-12 63.5
MsG0280010464.01.T01 MTR_4g056290 62.500 48 17 1 20 66 4 51 4.13e-12 59.7
MsG0280010464.01.T01 MTR_8g022810 52.308 65 28 2 29 91 41 104 1.53e-11 60.8
MsG0280010464.01.T01 MTR_8g022810 52.308 65 28 2 29 91 41 104 2.47e-11 60.1
MsG0280010464.01.T01 MTR_7g116510 32.308 130 83 3 9 136 7 133 2.60e-11 60.1
MsG0280010464.01.T01 MTR_3g435540 33.628 113 64 4 9 111 1 112 3.29e-11 60.1
MsG0280010464.01.T01 MTR_5g044210 65.000 40 14 0 24 63 64 103 4.78e-11 56.6
MsG0280010464.01.T01 MTR_7g008060 29.851 134 76 4 7 136 9 128 7.55e-11 58.9
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MsG0280010464.01.T01 AT5G22810 67.717 127 40 1 12 137 17 143 6.29e-54 173
MsG0280010464.01.T01 AT5G03820 55.556 135 51 2 11 136 7 141 3.83e-42 143
MsG0280010464.01.T01 AT5G03820 55.556 135 51 2 11 136 35 169 1.17e-41 142
MsG0280010464.01.T01 AT5G03810 62.712 118 43 1 20 136 21 138 3.45e-41 140
MsG0280010464.01.T01 AT3G16370 60.714 112 43 1 26 136 27 138 7.65e-40 137
MsG0280010464.01.T01 AT3G53100 53.077 130 59 2 8 136 9 137 3.01e-38 132
MsG0280010464.01.T01 AT3G43570 44.361 133 71 2 8 137 7 139 2.69e-30 111
MsG0280010464.01.T01 AT1G75900 44.361 133 71 2 8 137 20 152 1.55e-29 110
MsG0280010464.01.T01 AT5G42170 47.826 115 56 2 27 137 48 162 2.32e-29 110
MsG0280010464.01.T01 AT3G43550 43.609 133 72 3 8 137 7 139 1.17e-28 106
MsG0280010464.01.T01 AT1G58520 47.748 111 57 1 27 136 28 138 3.38e-28 106
MsG0280010464.01.T01 AT1G58525 47.748 111 57 1 27 136 28 138 3.38e-28 106
MsG0280010464.01.T01 AT1G59406 44.615 130 69 3 10 136 9 138 4.07e-28 106
MsG0280010464.01.T01 AT1G59030 44.615 130 69 3 10 136 9 138 4.07e-28 106
MsG0280010464.01.T01 AT1G58725 44.615 130 69 3 10 136 9 138 4.07e-28 106
MsG0280010464.01.T01 AT2G04570 41.985 131 75 1 7 136 6 136 1.49e-27 104
MsG0280010464.01.T01 AT3G14820 39.855 138 76 2 7 137 4 141 2.49e-27 104
MsG0280010464.01.T01 AT1G75890 41.791 134 73 3 8 136 23 156 1.87e-25 99.4
MsG0280010464.01.T01 AT1G75890 41.791 134 73 3 8 136 52 185 2.54e-25 99.4
MsG0280010464.01.T01 AT2G42990 42.342 111 63 1 27 136 26 136 1.52e-24 96.7
MsG0280010464.01.T01 AT1G20130 42.857 112 63 1 27 137 202 313 9.92e-24 95.9
MsG0280010464.01.T01 AT5G45960 41.228 114 66 1 27 139 45 158 9.97e-24 94.7
MsG0280010464.01.T01 AT2G40250 43.590 117 63 2 24 138 31 146 1.20e-23 94.4
MsG0280010464.01.T01 AT1G75890 38.514 148 73 3 8 137 23 170 1.51e-23 94.4
MsG0280010464.01.T01 AT1G75880 43.750 112 62 1 27 137 50 161 1.94e-23 94.0
MsG0280010464.01.T01 AT4G26790 42.727 110 62 1 28 136 28 137 3.20e-23 93.2
MsG0280010464.01.T01 AT4G26790 42.727 110 62 1 28 136 28 137 3.20e-23 93.2
MsG0280010464.01.T01 AT1G20130 42.857 112 63 1 27 137 182 293 4.93e-23 94.0
MsG0280010464.01.T01 AT1G20120 40.541 111 65 1 28 137 77 187 8.72e-23 92.8
MsG0280010464.01.T01 AT1G75880 43.363 113 62 2 27 137 50 162 7.86e-22 89.7
MsG0280010464.01.T01 AT5G63170 39.062 128 77 1 10 136 9 136 1.27e-21 89.0
MsG0280010464.01.T01 AT1G29660 42.188 128 67 4 12 137 16 138 2.45e-21 88.2
MsG0280010464.01.T01 AT1G20132 50.617 81 40 0 28 108 60 140 3.38e-21 88.2
MsG0280010464.01.T01 AT1G75920 38.583 127 76 2 11 136 12 137 4.33e-21 87.4
MsG0280010464.01.T01 AT1G73610 40.458 131 75 2 10 137 14 144 7.21e-21 86.7
MsG0280010464.01.T01 AT1G29670 40.909 132 70 4 9 136 10 137 9.35e-21 86.7
MsG0280010464.01.T01 AT2G03980 55.128 78 34 1 28 105 42 118 1.42e-20 86.3
MsG0280010464.01.T01 AT2G03980 55.128 78 34 1 28 105 42 118 1.42e-20 86.3
MsG0280010464.01.T01 AT2G03980 55.128 78 34 1 28 105 42 118 1.42e-20 86.3
MsG0280010464.01.T01 AT2G03980 55.128 78 34 1 28 105 42 118 1.42e-20 86.3
MsG0280010464.01.T01 AT1G29670 40.909 132 70 4 9 136 78 205 1.92e-20 86.3
MsG0280010464.01.T01 AT5G18430 37.594 133 75 3 8 136 9 137 2.91e-20 85.5
MsG0280010464.01.T01 AT1G75910 39.837 123 71 2 15 136 16 136 3.04e-20 85.1
MsG0280010464.01.T01 AT4G10950 45.783 83 44 1 25 107 67 148 1.27e-19 84.0
MsG0280010464.01.T01 AT5G15720 50.602 83 38 2 24 106 25 104 2.42e-19 82.4
MsG0280010464.01.T01 AT5G15720 50.602 83 38 2 24 106 25 104 4.79e-19 82.0
MsG0280010464.01.T01 AT1G33811 43.590 117 61 4 24 138 29 142 1.28e-18 80.9
MsG0280010464.01.T01 AT1G06990 34.513 113 73 1 26 137 34 146 2.47e-18 80.1
MsG0280010464.01.T01 AT5G08460 42.424 99 53 2 11 106 28 125 2.99e-18 80.1
MsG0280010464.01.T01 AT5G45950 37.931 116 70 2 27 141 38 152 8.59e-18 78.6
MsG0280010464.01.T01 AT1G75920 34.266 143 77 3 11 137 7 148 1.00e-17 78.2
MsG0280010464.01.T01 AT2G04020 47.500 80 37 2 28 105 42 118 1.09e-17 77.0
MsG0280010464.01.T01 AT1G20135 33.577 137 90 1 1 136 1 137 1.68e-17 77.4
MsG0280010464.01.T01 AT2G19060 40.157 127 72 4 11 136 13 136 2.54e-17 77.0
MsG0280010464.01.T01 AT2G04020 47.500 80 37 2 28 105 42 118 2.60e-17 77.0
MsG0280010464.01.T01 AT5G45670 37.736 106 63 2 1 106 1 103 4.83e-17 76.6
MsG0280010464.01.T01 AT4G10950 44.578 83 41 2 25 107 67 144 6.52e-17 76.3
MsG0280010464.01.T01 AT5G41890 37.931 116 66 3 31 141 33 147 6.94e-17 76.3
MsG0280010464.01.T01 AT2G19010 35.252 139 80 4 4 138 6 138 9.94e-17 75.5
MsG0280010464.01.T01 AT3G50400 48.235 85 42 1 24 106 29 113 1.47e-16 75.1
MsG0280010464.01.T01 AT2G24560 37.607 117 71 2 23 137 28 144 1.55e-16 74.7
MsG0280010464.01.T01 AT2G19010 35.252 139 80 4 4 138 36 168 1.80e-16 75.1
MsG0280010464.01.T01 AT2G24560 37.607 117 71 2 23 137 28 144 1.81e-16 74.7
MsG0280010464.01.T01 AT4G18970 46.429 84 42 2 23 106 22 102 1.96e-16 74.7
MsG0280010464.01.T01 AT4G18970 46.429 84 42 2 23 106 22 102 2.12e-16 74.7
MsG0280010464.01.T01 AT4G16230 36.667 150 76 7 7 142 2 146 2.88e-16 73.2
MsG0280010464.01.T01 AT5G55050 60.000 60 23 1 27 85 37 96 4.31e-16 73.9
MsG0280010464.01.T01 AT2G30310 37.069 116 71 2 24 137 29 144 4.99e-16 73.6
MsG0280010464.01.T01 AT1G71250 43.878 98 53 2 9 106 21 116 5.32e-16 73.6
MsG0280010464.01.T01 AT1G71691 38.793 116 65 4 23 136 49 160 8.35e-16 72.8
MsG0280010464.01.T01 AT2G30220 34.848 132 84 2 8 137 12 143 9.75e-16 72.8
MsG0280010464.01.T01 AT1G75930 32.824 131 86 2 9 137 9 139 1.04e-15 72.8
MsG0280010464.01.T01 AT1G71691 38.793 116 65 4 23 136 49 160 1.43e-15 72.4
MsG0280010464.01.T01 AT1G53920 35.802 162 76 8 1 142 7 160 1.49e-15 72.4
MsG0280010464.01.T01 AT2G31540 37.069 116 71 2 24 137 30 145 1.60e-15 72.4
MsG0280010464.01.T01 AT1G58430 35.294 119 75 2 21 137 27 145 3.01e-15 71.6
MsG0280010464.01.T01 AT2G23540 48.750 80 39 1 29 106 48 127 1.61e-14 69.3
MsG0280010464.01.T01 AT4G16220 42.708 96 53 1 12 107 13 106 1.91e-14 68.2
MsG0280010464.01.T01 AT5G37690 37.864 103 59 3 8 106 3 104 2.87e-14 68.6
MsG0280010464.01.T01 AT3G04290 34.351 131 82 3 9 136 8 137 4.31e-14 68.2
MsG0280010464.01.T01 AT1G23500 40.517 116 68 1 22 136 28 143 1.24e-13 67.0
MsG0280010464.01.T01 AT1G71120 43.333 90 45 4 20 108 22 106 3.18e-13 65.9
MsG0280010464.01.T01 AT4G28780 38.318 107 56 2 10 107 3 108 5.01e-13 65.1
MsG0280010464.01.T01 AT1G75920 45.588 68 36 1 11 78 12 78 5.32e-13 65.1
MsG0280010464.01.T01 AT2G19050 35.652 115 65 3 27 137 29 138 6.41e-13 64.3
MsG0280010464.01.T01 AT2G19050 35.652 115 65 3 27 137 29 138 9.03e-13 64.3
MsG0280010464.01.T01 AT5G33370 34.559 136 79 4 12 140 10 142 1.20e-12 63.9
MsG0280010464.01.T01 AT5G33370 34.559 136 79 4 12 140 10 142 1.31e-12 63.9
MsG0280010464.01.T01 AT4G30140 38.261 115 68 3 27 140 31 143 3.95e-12 62.8
MsG0280010464.01.T01 AT1G74460 35.398 113 62 4 9 111 1 112 2.17e-11 60.5

Find 37 sgRNAs with CRISPR-Local

Find 49 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
CACTTTGCTTCTGGTGCTTC+TGG 0.160134 2:-69982317 MsG0280010464.01.T01:CDS
GTTCCTGCATTGTTCATATT+TGG 0.193206 2:-69982680 MsG0280010464.01.T01:CDS
AAATCAAATTTCCCTCCTTA+TGG 0.264671 2:-69982608 MsG0280010464.01.T01:CDS
AAATGGTGCCACTTTGCTTC+TGG 0.283049 2:-69982326 MsG0280010464.01.T01:CDS
TACCTCAACTTACAGGAAAA+AGG 0.290453 2:-69982361 MsG0280010464.01.T01:CDS
GCAAAGTGGCACCATTTAAC+AGG 0.328489 2:+69982332 None:intergenic
GTAGATTGGTTTAAAGTATT+GGG 0.334079 2:+69982852 None:intergenic
GTAGTGTTCAATGTGGTTAA+AGG 0.337388 2:-69982713 MsG0280010464.01.T01:CDS
ACCAGCTTACCTCAACTTAC+AGG 0.339262 2:-69982368 MsG0280010464.01.T01:CDS
TGTAGATTGGTTTAAAGTAT+TGG 0.357603 2:+69982851 None:intergenic
TCCTGTAAGTTGAGGTAAGC+TGG 0.400188 2:+69982367 None:intergenic
CTACTCCTACCAATATATTC+TGG 0.408033 2:+69982129 None:intergenic
ATAAGAATGCCAGAATATAT+TGG 0.411516 2:-69982138 MsG0280010464.01.T01:CDS
TTCAACAATCAAATGGCAAC+AGG 0.446449 2:-69982578 MsG0280010464.01.T01:CDS
TTGTTGAAGTCTCTTCCATA+AGG 0.474228 2:+69982593 None:intergenic
GGAGATTCTGTTGTTGATGC+AGG 0.491170 2:-69982659 MsG0280010464.01.T01:CDS
ATATATATTTACATATAGCT+TGG 0.493884 2:+69982279 None:intergenic
GGTCTAGCATGCAATCTCAT+TGG 0.499215 2:-69982179 MsG0280010464.01.T01:intron
AGAGAAAGTGAGAGAAAGAG+AGG 0.502854 2:+69982825 None:intergenic
AAGAGACTTCAACAATCAAA+TGG 0.505945 2:-69982585 MsG0280010464.01.T01:CDS
AATCTCATTGGAGCAGCAGC+TGG 0.525144 2:-69982167 MsG0280010464.01.T01:CDS
ATTACTGCAGCTGAAAACCT+TGG 0.536216 2:-69982406 MsG0280010464.01.T01:intron
CAACCTTTGCTACACACTGC+AGG 0.549941 2:-69982921 None:intergenic
TCTCCAAATATGAACAATGC+AGG 0.555612 2:+69982677 None:intergenic
TTGAAGTCTCTTCCATAAGG+AGG 0.564229 2:+69982596 None:intergenic
TGAAGTCTCTTCCATAAGGA+GGG 0.575908 2:+69982597 None:intergenic
ATGAACAATGCAGGAACAAG+AGG 0.577996 2:+69982686 None:intergenic
TTTACATATAGCTTGGCTGT+AGG 0.578847 2:+69982286 None:intergenic
GAAGTGATGATCTGAGGAAA+AGG 0.584382 2:+69982874 None:intergenic
GTGGATAAGTAGTAAATCCA+AGG 0.588411 2:+69982389 None:intergenic
ACAATCAAATGGCAACAGGA+AGG 0.600840 2:-69982574 MsG0280010464.01.T01:CDS
CAGAAGCACCAGAAGCAAAG+TGG 0.605307 2:+69982318 None:intergenic
TATTGGGAAGTGATGATCTG+AGG 0.619043 2:+69982868 None:intergenic
GAATGCCAGAATATATTGGT+AGG 0.626551 2:-69982134 None:intergenic
GTGATGATCTGAGGAAAAGG+TGG 0.635552 2:+69982877 None:intergenic
TCTGTTTGTAGTGTTCAATG+TGG 0.664925 2:-69982720 MsG0280010464.01.T01:CDS
TGTAAGTTGAGGTAAGCTGG+TGG 0.679587 2:+69982370 None:intergenic

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!! ATAAAAGAAGAAAACAAATT+AGG + Chr2:69982835-69982854 None:intergenic 15.0%
!! ATATATATTTACATATAGCT+TGG + Chr2:69982769-69982788 None:intergenic 15.0%
!! ATTTGAAAGAAAGAAAAAAA+TGG - Chr2:69982268-69982287 MsG0280010464.01.T01:intron 15.0%
!! AAACAATAATGACCTAAATA+AGG - Chr2:69982587-69982606 MsG0280010464.01.T01:CDS 20.0%
!!! CAAAAATCATATCCTTATTT+AGG + Chr2:69982602-69982621 None:intergenic 20.0%
! AAAGGAAAAAACCTGTTAAA+TGG - Chr2:69982702-69982721 MsG0280010464.01.T01:CDS 25.0%
! ATAAGAATGCCAGAATATAT+TGG - Chr2:69982907-69982926 MsG0280010464.01.T01:five_prime_UTR 25.0%
! CTACTTAACTTTGATCATAT+TGG - Chr2:69982804-69982823 MsG0280010464.01.T01:five_prime_UTR 25.0%
!!! CTTCTTTTATTTGTTCCATT+GGG - Chr2:69982845-69982864 MsG0280010464.01.T01:five_prime_UTR 25.0%
!!! GTAGATTGGTTTAAAGTATT+GGG + Chr2:69982196-69982215 None:intergenic 25.0%
!!! TCTTCTTTTATTTGTTCCAT+TGG - Chr2:69982844-69982863 MsG0280010464.01.T01:five_prime_UTR 25.0%
!!! TGTAGATTGGTTTAAAGTAT+TGG + Chr2:69982197-69982216 None:intergenic 25.0%
AAATCAAATTTCCCTCCTTA+TGG - Chr2:69982437-69982456 MsG0280010464.01.T01:intron 30.0%
AAGAGACTTCAACAATCAAA+TGG - Chr2:69982460-69982479 MsG0280010464.01.T01:intron 30.0%
GTAGTGTTCAATGTGGTTAA+AGG - Chr2:69982332-69982351 MsG0280010464.01.T01:CDS 35.0%
GTGGATAAGTAGTAAATCCA+AGG + Chr2:69982659-69982678 None:intergenic 35.0%
GTTCCTGCATTGTTCATATT+TGG - Chr2:69982365-69982384 MsG0280010464.01.T01:CDS 35.0%
TAACTACATGCATGCAAAGA+AGG + Chr2:69982544-69982563 None:intergenic 35.0%
TACCTCAACTTACAGGAAAA+AGG - Chr2:69982684-69982703 MsG0280010464.01.T01:CDS 35.0%
TCTCCAAATATGAACAATGC+AGG + Chr2:69982371-69982390 None:intergenic 35.0%
TCTGTTTGTAGTGTTCAATG+TGG - Chr2:69982325-69982344 MsG0280010464.01.T01:CDS 35.0%
TTCAACAATCAAATGGCAAC+AGG - Chr2:69982467-69982486 MsG0280010464.01.T01:intron 35.0%
TTGTTGAAGTCTCTTCCATA+AGG + Chr2:69982455-69982474 None:intergenic 35.0%
TTTACATATAGCTTGGCTGT+AGG + Chr2:69982762-69982781 None:intergenic 35.0%
! AAACTTGCAGCAGATTTTAC+AGG - Chr2:69982506-69982525 MsG0280010464.01.T01:intron 35.0%
! TTCCTTTTTCCTGTAAGTTG+AGG + Chr2:69982689-69982708 None:intergenic 35.0%
!! GAAAGAGAGGTTTTGTAGAT+TGG + Chr2:69982210-69982229 None:intergenic 35.0%
ACAATCAAATGGCAACAGGA+AGG - Chr2:69982471-69982490 MsG0280010464.01.T01:intron 40.0%
AGAGAAAGTGAGAGAAAGAG+AGG + Chr2:69982223-69982242 None:intergenic 40.0%
ATGAACAATGCAGGAACAAG+AGG + Chr2:69982362-69982381 None:intergenic 40.0%
ATTACTGCAGCTGAAAACCT+TGG - Chr2:69982639-69982658 MsG0280010464.01.T01:CDS 40.0%
GAAGTGATGATCTGAGGAAA+AGG + Chr2:69982174-69982193 None:intergenic 40.0%
TATTGGGAAGTGATGATCTG+AGG + Chr2:69982180-69982199 None:intergenic 40.0%
TGAAGTCTCTTCCATAAGGA+GGG + Chr2:69982451-69982470 None:intergenic 40.0%
TTGAAGTCTCTTCCATAAGG+AGG + Chr2:69982452-69982471 None:intergenic 40.0%
! AAGGTTTTCAGCTGCAGTAA+TGG + Chr2:69982640-69982659 None:intergenic 40.0%
!! GCAACAGGAAGGTTTTGTAA+TGG - Chr2:69982482-69982501 MsG0280010464.01.T01:intron 40.0%
ACCAGCTTACCTCAACTTAC+AGG - Chr2:69982677-69982696 MsG0280010464.01.T01:CDS 45.0%
AGATTGCATGCTAGACCCAA+TGG + Chr2:69982863-69982882 None:intergenic 45.0%
GCAAAGTGGCACCATTTAAC+AGG + Chr2:69982716-69982735 None:intergenic 45.0%
GGAGATTCTGTTGTTGATGC+AGG - Chr2:69982386-69982405 MsG0280010464.01.T01:CDS 45.0%
GGTCTAGCATGCAATCTCAT+TGG - Chr2:69982866-69982885 MsG0280010464.01.T01:five_prime_UTR 45.0%
GTGATGATCTGAGGAAAAGG+TGG + Chr2:69982171-69982190 None:intergenic 45.0%
! TCCTGTAAGTTGAGGTAAGC+TGG + Chr2:69982681-69982700 None:intergenic 45.0%
! TGTAAGTTGAGGTAAGCTGG+TGG + Chr2:69982678-69982697 None:intergenic 45.0%
!! AAATGGTGCCACTTTGCTTC+TGG - Chr2:69982719-69982738 MsG0280010464.01.T01:CDS 45.0%
AATCTCATTGGAGCAGCAGC+TGG - Chr2:69982878-69982897 MsG0280010464.01.T01:five_prime_UTR 50.0%
CACTTTGCTTCTGGTGCTTC+TGG - Chr2:69982728-69982747 MsG0280010464.01.T01:CDS 50.0%
!! CAGAAGCACCAGAAGCAAAG+TGG + Chr2:69982730-69982749 None:intergenic 50.0%
Chromosome Type Strat End Strand Name
Chr2 gene 69982135 69982932 69982135 ID=MsG0280010464.01;Name=MsG0280010464.01
Chr2 mRNA 69982135 69982932 69982135 ID=MsG0280010464.01.T01;Parent=MsG0280010464.01;Name=MsG0280010464.01.T01;_AED=0.13;_eAED=0.13;_QI=152|0.75|0.8|1|0|0|5|0|142
Chr2 exon 69982135 69982194 69982135 ID=MsG0280010464.01.T01:exon:26834;Parent=MsG0280010464.01.T01
Chr2 exon 69982291 69982418 69982291 ID=MsG0280010464.01.T01:exon:26833;Parent=MsG0280010464.01.T01
Chr2 exon 69982540 69982932 69982540 ID=MsG0280010464.01.T01:exon:26832;Parent=MsG0280010464.01.T01
Chr2 five_prime_UTR 69982781 69982932 69982781 ID=MsG0280010464.01.T01:five_prime_utr;Parent=MsG0280010464.01.T01
Chr2 CDS 69982540 69982780 69982540 ID=MsG0280010464.01.T01:cds;Parent=MsG0280010464.01.T01
Chr2 CDS 69982291 69982418 69982291 ID=MsG0280010464.01.T01:cds;Parent=MsG0280010464.01.T01
Chr2 CDS 69982135 69982194 69982135 ID=MsG0280010464.01.T01:cds;Parent=MsG0280010464.01.T01
Gene Sequence

>MsG0280010464.01.T01

ATGGAGCATTCAACTTATAATTTCTTGACATTTTTGCTTCTGTTTGTAGTGTTCAATGTGGTTAAAGGACAGCCTCTTGTTCCTGCATTGTTCATATTTGGAGATTCTGTTGTTGATGCAGGAAATAACAATAACTTATACACAATTGTTAAATCAAATTTCCCTCCTTATGGAAGAGACTTCAACAATCAAATGGCAACAGGAAGGTTTTGTAATGGAAAACTTGCAGCAGATTTTACAGCTGAAAACCTTGGATTTACTACTTATCCACCAGCTTACCTCAACTTACAGGAAAAAGGAAAAAACCTGTTAAATGGTGCCACTTTGCTTCTGGTGCTTCTGGACTTTGATCCTACAGCCAAGCTATATCATGCAATCTCATTGGAGCAGCAGCTGGAACACTATAAGAATGCCAGAATATATTGGTAG
Protein sequence

>MsG0280010464.01.T01

MEHSTYNFLTFLLLFVVFNVVKGQPLVPALFIFGDSVVDAGNNNNLYTIVKSNFPPYGRDFNNQMATGRFCNGKLAADFTAENLGFTTYPPAYLNLQEKGKNLLNGATLLLVLLDFDPTAKLYHAISLEQQLEHYKNARIYW*