AlfalfaGEDB | MsG0280009416.01

M. sativa cultivar ZhongmuNo.1 / MsG0280009416.01

KEGG: -
GO: GO:0006869 GO:0008289
PFAM: PF00234

Nr:

Query id	Subject id	identity %	alignment length	mis matches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MsG0280009416.01.T01	XP_039686545.1	97.345	113	3	0	1	113	1	113	4.56E-69	213

Swissprot:

Query id	Subject id	identity %	alignment length	mis matches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MsG0280009416.01.T01	Q9ZPW9	53.271	107	50	0	6	112	7	113	8.75E-36	121

Trembl:

Query id	Subject id	identity %	alignment length	mis matches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MsG0280009416.01.T01	G7IQQ8	97.345	113	3	0	1	113	1	113	2.18e-69	213

TFs/TRs:

Gene ID	Type	Classification

Protein Kinases:

Gene ID	Type	Classification

Network:

Co-expression Network

Gene1	Gene2	correlation coefficient	p_value	FDR
MsG0180001104.01	MsG0280009416.01	0.847588	1.051992e-59	1.878851e-56
MsG0180001801.01	MsG0280009416.01	0.844150	9.036036e-59	1.445743e-55
MsG0180001802.01	MsG0280009416.01	0.849090	4.041181e-60	7.575598e-57
MsG0180003955.01	MsG0280009416.01	0.846455	2.149278e-59	3.701850e-56
MsG0180004949.01	MsG0280009416.01	0.809029	2.314955e-50	1.344396e-47
MsG0280006485.01	MsG0280009416.01	0.844561	7.004176e-59	1.135265e-55
MsG0280007108.01	MsG0280009416.01	0.801107	1.052475e-48	4.984698e-46
MsG0280007686.01	MsG0280009416.01	0.803786	2.952262e-49	1.496933e-46
MsG0280009416.01	MsG0280010296.01	0.846012	2.837275e-59	4.816978e-56
MsG0280009416.01	MsG0280010464.01	0.812629	3.851075e-51	2.460927e-48
MsG0280009416.01	MsG0280010502.01	0.874167	8.342100e-68	3.717179e-64
MsG0280009416.01	MsG0380013406.01	0.813845	2.082755e-51	1.375194e-48
MsG0280009416.01	MsG0380013409.01	0.804019	2.641265e-49	1.347287e-46
MsG0280009416.01	MsG0380017582.01	0.832012	1.205208e-55	1.330294e-52
MsG0280009416.01	MsG0380017618.01	0.833065	6.605963e-56	7.523782e-53
MsG0280009416.01	MsG0480020722.01	0.827360	1.632895e-54	1.573103e-51
MsG0280009416.01	MsG0580027036.01	0.816143	6.436791e-52	4.524440e-49
MsG0280009416.01	MsG0580028023.01	0.861861	7.618771e-64	2.186483e-60
MsG0280009416.01	MsG0580028924.01	0.838439	2.873354e-57	3.848830e-54
MsG0280009416.01	MsG0780037094.01	0.842583	2.367179e-58	3.605528e-55
MsG0280009416.01	MsG0780037751.01	0.805924	1.055260e-49	5.653119e-47
MsG0280009416.01	MsG0880043261.01	0.834684	2.599814e-56	3.107653e-53
MsG0280009416.01	MsG0880043262.01	0.809031	2.313456e-50	1.343568e-47
MsG0280009416.01	MsG0880043961.01	0.825121	5.569411e-54	5.031052e-51
MsG0280009416.01	MsG0880043989.01	0.821023	5.032800e-53	4.047916e-50
MsG0280009416.01	MsG0880045256.01	0.808566	2.907931e-50	1.668229e-47
MsG0280009416.01	MsG0880045790.01	0.833612	4.824754e-56	5.585100e-53
MsG0280009416.01	MsG0880046463.01	0.804480	2.116570e-49	1.092479e-46

PPI

Gene1	Gene2	Type

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MsG0280009416.01.T01	MTR_2g062600	97.345	113	3	0	1	113	1	113	5.52e-73	213
MsG0280009416.01.T01	MTR_4g428370	45.133	113	60	2	1	112	58	169	1.53e-25	94.4
MsG0280009416.01.T01	MTR_4g027800	41.593	113	64	2	1	112	1	112	4.72e-23	86.7
MsG0280009416.01.T01	MTR_4g027840	41.593	113	64	2	1	112	1	112	4.76e-23	87.0
MsG0280009416.01.T01	MTR_4g028180	37.288	118	68	2	1	112	6	123	3.66e-21	82.0
MsG0280009416.01.T01	MTR_4g029390	38.095	105	63	2	9	112	10	113	9.45e-21	80.5
MsG0280009416.01.T01	MTR_4g029350	39.815	108	62	3	6	112	8	113	6.17e-19	75.9
MsG0280009416.01.T01	MTR_7g073170	34.615	104	68	0	10	113	13	116	8.89e-19	75.9
MsG0280009416.01.T01	MTR_4g029210	36.607	112	65	4	6	113	4	113	6.60e-18	73.6
MsG0280009416.01.T01	MTR_7g073130	37.168	113	68	1	1	113	7	116	3.01e-17	72.0
MsG0280009416.01.T01	MTR_4g028190	29.907	107	74	1	8	113	13	119	2.86e-16	69.3
MsG0280009416.01.T01	MTR_7g073150	30.172	116	77	2	1	112	1	116	4.42e-16	69.3
MsG0280009416.01.T01	MTR_7g073120	33.913	115	71	3	1	113	8	119	1.19e-15	68.6
MsG0280009416.01.T01	MTR_7g072900	34.211	114	71	2	1	113	7	117	5.68e-14	63.2
MsG0280009416.01.T01	MTR_1g040335	36.283	113	68	2	2	113	8	117	6.08e-14	63.2
MsG0280009416.01.T01	MTR_7g072930	32.000	100	67	1	14	112	17	116	6.25e-14	63.9
MsG0280009416.01.T01	MTR_7g072730	31.193	109	73	2	6	112	9	117	7.60e-14	64.3
MsG0280009416.01.T01	MTR_7g072830	32.174	115	73	3	1	113	7	118	2.78e-13	61.6
MsG0280009416.01.T01	MTR_4g028310	30.172	116	78	2	1	113	1	116	4.24e-13	61.6
MsG0280009416.01.T01	MTR_7g072980	34.783	115	70	3	1	113	7	118	1.11e-12	60.5
MsG0280009416.01.T01	MTR_7g451500	31.868	91	61	1	24	113	7	97	1.81e-12	58.9
MsG0280009416.01.T01	MTR_3g467100	27.778	108	74	3	8	113	7	112	1.81e-12	59.3
MsG0280009416.01.T01	MTR_7g072810	33.043	115	66	4	1	113	7	112	2.43e-11	56.6
MsG0280009416.01.T01	MTR_3g056640	25.424	118	82	3	1	112	2	119	4.52e-11	55.8
MsG0280009416.01.T01	MTR_7g073100	33.333	114	72	2	1	113	7	117	4.55e-11	56.6
MsG0280009416.01.T01	MTR_7g072760	32.692	104	68	2	12	113	17	120	5.54e-11	55.8

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MsG0280009416.01.T01	AT2G18370	53.271	107	50	0	6	112	7	113	8.92e-37	121
MsG0280009416.01.T01	AT5G59320	41.593	113	64	2	1	112	1	112	3.02e-22	84.3
MsG0280009416.01.T01	AT5G59310	36.607	112	68	2	1	112	1	109	8.97e-21	80.5
MsG0280009416.01.T01	AT3G51590	39.216	102	60	2	11	111	12	112	9.62e-19	75.5
MsG0280009416.01.T01	AT5G01870	39.048	105	60	2	11	112	10	113	4.11e-18	73.9
MsG0280009416.01.T01	AT3G08770	39.785	93	52	2	22	111	18	109	3.84e-17	71.2
MsG0280009416.01.T01	AT3G08770	39.785	93	52	2	22	111	18	109	4.47e-17	71.2
MsG0280009416.01.T01	AT2G38540	34.513	113	69	3	1	111	5	114	1.87e-15	67.4
MsG0280009416.01.T01	AT4G33355	35.577	104	64	3	11	113	16	117	2.31e-15	67.0
MsG0280009416.01.T01	AT2G15050	32.407	108	67	3	3	104	2	109	3.25e-15	66.6
MsG0280009416.01.T01	AT2G15050	32.407	108	67	3	3	104	2	109	3.27e-15	66.6
MsG0280009416.01.T01	AT2G15050	32.407	108	67	3	3	104	2	109	3.46e-15	66.6
MsG0280009416.01.T01	AT4G33355	35.922	103	63	3	11	112	16	116	9.15e-15	65.5
MsG0280009416.01.T01	AT3G51600	33.333	114	70	3	4	112	3	115	2.19e-14	64.3

Find 24 sgRNAs with CRISPR-Local

Find 25 sgRNAs with CRISPR-GE

CRISPR-Local

CRISPR-Local
sgRNA_sequence	on_target_score	Position	Region
TTCATGCAGTGATGTGATTA+AGG	0.266904	2:+51637726	MsG0280009416.01.T01:CDS
TGCAATTAAAGAAGATTTCA+TGG	0.308768	2:-51637650	None:intergenic
CAATTGGCTCAGGCTCTTGC+AGG	0.325545	2:+51637913	MsG0280009416.01.T01:CDS
GAAACCTTGTGTGAGCTATT+TGG	0.326436	2:+51637753	MsG0280009416.01.T01:CDS
TACTTGGAACAATCAGCATC+TGG	0.351088	2:-51637970	None:intergenic
CCACCAGCTGCATGTTGCTC+TGG	0.412892	2:+51637793	MsG0280009416.01.T01:CDS
AATTGAAGCCTCTGAATTTG+AGG	0.422177	2:-51637704	None:intergenic
AGCTATTTGGTCAGTGGAAG+TGG	0.469665	2:+51637766	MsG0280009416.01.T01:CDS
TATTAAAATCAACTCACAAT+TGG	0.472842	2:+51637897	MsG0280009416.01.T01:CDS
AATCAACTCACAATTGGCTC+AGG	0.514463	2:+51637903	MsG0280009416.01.T01:CDS
ACTTGGAACAATCAGCATCT+GGG	0.519962	2:-51637969	None:intergenic
CTTTGTTGCCTCAAATTCAG+AGG	0.523992	2:+51637696	MsG0280009416.01.T01:CDS
GCTCCAGAGCAACATGCAGC+TGG	0.542595	2:-51637796	None:intergenic
CAGGCTCTTGCAGGGAACTG+TGG	0.566184	2:+51637922	MsG0280009416.01.T01:CDS
AGCATCTGGGGAGATACTGA+TGG	0.572323	2:-51637956	None:intergenic
GCATCTGGGGAGATACTGAT+GGG	0.583176	2:-51637955	None:intergenic
AATTGGCTCAGGCTCTTGCA+GGG	0.601314	2:+51637914	MsG0280009416.01.T01:CDS
CTTGGAACAATCAGCATCTG+GGG	0.606095	2:-51637968	None:intergenic
CCAGAGCAACATGCAGCTGG+TGG	0.627457	2:-51637793	None:intergenic
ATCAGCTGTATCAACATCTG+AGG	0.627591	2:+51637831	MsG0280009416.01.T01:CDS
TGTGTGAGCTATTTGGTCAG+TGG	0.644010	2:+51637760	MsG0280009416.01.T01:CDS
CTGACCAAATAGCTCACACA+AGG	0.667347	2:-51637757	None:intergenic
ATCTGGGGAGATACTGATGG+GGG	0.675132	2:-51637953	None:intergenic
CATCTGGGGAGATACTGATG+GGG	0.712150	2:-51637954	None:intergenic

CRISPR-GE

badsite warning	sgRNA_sequence	Strand	Position	Region	GC_content
!!	TATTAAAATCAACTCACAAT+TGG	+	Chr2:51637897-51637916	MsG0280009416.01.T01:CDS	20.0%
!!!	TGAGTTGATTTTAATACTCT+TGG	-	Chr2:51637893-51637912	None:intergenic	25.0%
!!	GATTTTAATACTCTTGGAAG+TGG	-	Chr2:51637887-51637906	None:intergenic	30.0%
	AATTGAAGCCTCTGAATTTG+AGG	-	Chr2:51637707-51637726	None:intergenic	35.0%
	TTCATGCAGTGATGTGATTA+AGG	+	Chr2:51637726-51637745	MsG0280009416.01.T01:CDS	35.0%
	ACTTGGAACAATCAGCATCT+GGG	-	Chr2:51637972-51637991	None:intergenic	40.0%
	ATCAGCTGTATCAACATCTG+AGG	+	Chr2:51637831-51637850	MsG0280009416.01.T01:CDS	40.0%
	CTTTGTTGCCTCAAATTCAG+AGG	+	Chr2:51637696-51637715	MsG0280009416.01.T01:CDS	40.0%
	GAAACCTTGTGTGAGCTATT+TGG	+	Chr2:51637753-51637772	MsG0280009416.01.T01:CDS	40.0%
	TACTTGGAACAATCAGCATC+TGG	-	Chr2:51637973-51637992	None:intergenic	40.0%
!	AATCAACTCACAATTGGCTC+AGG	+	Chr2:51637903-51637922	MsG0280009416.01.T01:CDS	40.0%
	AGCTATTTGGTCAGTGGAAG+TGG	+	Chr2:51637766-51637785	MsG0280009416.01.T01:CDS	45.0%
	CTGACCAAATAGCTCACACA+AGG	-	Chr2:51637760-51637779	None:intergenic	45.0%
	CTTGGAACAATCAGCATCTG+GGG	-	Chr2:51637971-51637990	None:intergenic	45.0%
	TGTGTGAGCTATTTGGTCAG+TGG	+	Chr2:51637760-51637779	MsG0280009416.01.T01:CDS	45.0%
	AGCATCTGGGGAGATACTGA+TGG	-	Chr2:51637959-51637978	None:intergenic	50.0%
	ATCTGGGGAGATACTGATGG+GGG	-	Chr2:51637956-51637975	None:intergenic	50.0%
	CATCTGGGGAGATACTGATG+GGG	-	Chr2:51637957-51637976	None:intergenic	50.0%
	GCATCTGGGGAGATACTGAT+GGG	-	Chr2:51637958-51637977	None:intergenic	50.0%
!	AATTGGCTCAGGCTCTTGCA+GGG	+	Chr2:51637914-51637933	MsG0280009416.01.T01:CDS	50.0%
!	CAATTGGCTCAGGCTCTTGC+AGG	+	Chr2:51637913-51637932	MsG0280009416.01.T01:CDS	55.0%
	CAGGCTCTTGCAGGGAACTG+TGG	+	Chr2:51637922-51637941	MsG0280009416.01.T01:CDS	60.0%
	CCACCAGCTGCATGTTGCTC+TGG	+	Chr2:51637793-51637812	MsG0280009416.01.T01:CDS	60.0%
	CCAGAGCAACATGCAGCTGG+TGG	-	Chr2:51637796-51637815	None:intergenic	60.0%
	GCTCCAGAGCAACATGCAGC+TGG	-	Chr2:51637799-51637818	None:intergenic	60.0%

Chromosome	Type	Strat	End	Strand	Name
Chr2	gene	51637652	51637993	51637652	ID=MsG0280009416.01;Name=MsG0280009416.01
Chr2	mRNA	51637652	51637993	51637652	ID=MsG0280009416.01.T01;Parent=MsG0280009416.01;Name=MsG0280009416.01.T01;_AED=0.38;_eAED=0.38;_QI=0\|-1\|0\|1\|-1\|1\|1\|0\|113
Chr2	exon	51637652	51637993	51637652	ID=MsG0280009416.01.T01:exon:6631;Parent=MsG0280009416.01.T01
Chr2	CDS	51637652	51637993	51637652	ID=MsG0280009416.01.T01:cds;Parent=MsG0280009416.01.T01

Gene Sequence	>MsG0280009416.01.T01 ATGAAATCTTCTTTAATTGCATTCGCTGCTCTTGTGCTTCTCATCTTTGTTGCCTCAAATTCAGAGGCTTCAATTTCATGCAGTGATGTGATTAAGGACTTGAAACCTTGTGTGAGCTATTTGGTCAGTGGAAGTGGACAACCACCAGCTGCATGTTGCTCTGGAGCTAAAGCTCTAGCATCAGCTGTATCAACATCTGAGGATAAAAAAGCTGCTTGCAACTGCATCAAATCCACTTCCAAGAGTATTAAAATCAACTCACAATTGGCTCAGGCTCTTGCAGGGAACTGTGGAATTAACACCCCCATCAGTATCTCCCCAGATGCTGATTGTTCCAAGTAA
Protein sequence	>MsG0280009416.01.T01 MKSSLIAFAALVLLIFVASNSEASISCSDVIKDLKPCVSYLVSGSGQPPAACCSGAKALASAVSTSEDKKAACNCIKSTSKSIKINSQLAQALAGNCGINTPISISPDADCSK*