AlfalfaGEDB | MsG0280010546.01

M. sativa cultivar ZhongmuNo.1 / MsG0280010546.01

KEGG: -
GO: -
PFAM: PF11250

Nr:

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Query id	Subject id	identity %	alignment length	mis matches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MsG0280010546.01.T01	MCH80388.1	54.348	184	58	3	137	294	122	305	3.48E-58	199

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Swissprot:

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Query id	Subject id	identity %	alignment length	mis matches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
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Trembl:

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Query id	Subject id	identity %	alignment length	mis matches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MsG0280010546.01.T01	A0A392M080	54.348	184	58	3	137	294	122	305	1.66e-58	199

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TFs/TRs:

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Gene ID	Type	Classification
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Protein Kinases:

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Gene ID	Type	Classification
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Network:

Co-expression Network

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Gene1	Gene2	correlation coefficient	p_value	FDR
MsG0080048969.01	MsG0280010546.01	0.802960	4.377082e-49	2.172802e-46
MsG0280009901.01	MsG0280010546.01	0.816305	5.922098e-52	4.181342e-49
MsG0280010523.01	MsG0280010546.01	0.819817	9.516619e-53	7.401365e-50
MsG0280010546.01	MsG0280011309.01	0.810044	1.401676e-50	8.362698e-48
MsG0280010546.01	MsG0380016033.01	0.806732	7.131640e-50	3.900119e-47
MsG0280010546.01	MsG0480019604.01	0.819461	1.147247e-52	8.834523e-50
MsG0280010546.01	MsG0480020229.01	0.801946	7.084253e-49	3.426852e-46
MsG0280010546.01	MsG0580025301.01	0.800458	1.428036e-48	6.652467e-46
MsG0280010546.01	MsG0680031996.01	0.812601	3.906054e-51	2.494186e-48
MsG0280010546.01	MsG0680032000.01	0.809824	1.562903e-50	9.270868e-48

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PPI

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Gene1	Gene2	Type
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Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MsG0280010546.01.T01	MTR_2g086590	90.164	61	6	0	14	74	136	196	3.72e-33	122
MsG0280010546.01.T01	MTR_5g038810	45.865	133	45	2	135	241	529	660	9.70e-30	119
MsG0280010546.01.T01	MTR_7g108440	44.961	129	46	1	137	240	658	786	1.47e-29	118
MsG0280010546.01.T01	MTR_5g021380	35.751	193	94	6	128	292	631	821	5.81e-25	105
MsG0280010546.01.T01	MTR_7g057470	35.503	169	96	2	137	292	349	517	1.60e-24	103
MsG0280010546.01.T01	MTR_7g057490	35.503	169	96	2	137	292	349	517	1.60e-24	103
MsG0280010546.01.T01	MTR_6g048560	35.915	142	90	1	152	292	43	184	6.47e-22	92.4
MsG0280010546.01.T01	MTR_6g016175	36.424	151	73	2	138	265	137	287	7.32e-21	92.8
MsG0280010546.01.T01	MTR_0465s0020	47.872	94	24	1	142	210	100	193	2.54e-19	85.5
MsG0280010546.01.T01	MTR_0032s0100	33.083	133	67	2	137	247	35	167	1.78e-16	76.3

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Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
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Find 108 sgRNAs with CRISPR-Local

Find 257 sgRNAs with CRISPR-GE

CRISPR-Local

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CRISPR-Local
sgRNA_sequence	on_target_score	Position	Region
AAACAAGTTGTTCAAGAAAT+TGG	0.234414	2:+71275561	MsG0280010546.01.T01:CDS
AAAGGTGGGAATGGAGGAAG+AGG	0.522906	2:+71274412	MsG0280010546.01.T01:CDS
AAATATGGCAATTCGGATTG+GGG	0.545866	2:+71275502	MsG0280010546.01.T01:CDS
AAGATGCGTGGTGTAATCAA+AGG	0.565847	2:+71275587	MsG0280010546.01.T01:CDS
AAGGATTCTTTGAGAAGGAA+AGG	0.505362	2:+71274394	MsG0280010546.01.T01:CDS
AAGGGTTAGTGGTTGGATGG+CGG	0.675064	2:+71275606	MsG0280010546.01.T01:CDS
AAGTTGCAAAAGATGTTACA+AGG	0.553919	2:+71274757	MsG0280010546.01.T01:CDS
AATGGTCCGAAATTGTTCTA+GGG	0.582714	2:-71275465	None:intergenic
AATTTCGGACCATTGCCCAT+TGG	0.563686	2:+71275474	MsG0280010546.01.T01:CDS
ACTCCAACTCTCCGAGATAA+GGG	0.470583	2:-71275410	None:intergenic

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CRISPR-GE

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sgRNA_sequence	Strand	Position	Region	GC_content
AAACTTTATGTCGTGGTTTA+TGG	+	Chr2:71274929-71274948	MsG0280010546.01.T01:intron	30.0%
AACGCAAAGTAATTAAGGAT+TGG	+	Chr2:71274846-71274865	MsG0280010546.01.T01:intron	30.0%
AACTTTATGTCGTGGTTTAT+GGG	+	Chr2:71274930-71274949	MsG0280010546.01.T01:intron	30.0%
AAGTTGCAAAAGATGTTACA+AGG	+	Chr2:71274757-71274776	MsG0280010546.01.T01:CDS	30.0%
AGTTGCAAAAGATGTTACAA+GGG	+	Chr2:71274758-71274777	MsG0280010546.01.T01:CDS	30.0%
ATGCATTCATTCGTTTAATG+CGG	+	Chr2:71274360-71274379	MsG0280010546.01.T01:intron	30.0%
ATTCTTATGGAGAATGTAAG+AGG	+	Chr2:71273443-71273462	MsG0280010546.01.T01:CDS	30.0%
GGGTTTATTTCAGATCTATT+AGG	+	Chr2:71273524-71273543	MsG0280010546.01.T01:CDS	30.0%
GGTTCTTAAATATGGCAATT+CGG	+	Chr2:71275495-71275514	MsG0280010546.01.T01:CDS	30.0%
TAAATATGGCAATTCGGATT+GGG	+	Chr2:71275501-71275520	MsG0280010546.01.T01:CDS	30.0%

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Chromosome	Type	Strat	End	Strand	Name
Chr2	gene	71273352	71275768	71273352	ID=MsG0280010546.01;Name=MsG0280010546.01
Chr2	mRNA	71273352	71275768	71273352	ID=MsG0280010546.01.T01;Parent=MsG0280010546.01;Name=MsG0280010546.01.T01;_AED=0.53;_eAED=0.54;_QI=0\|0\|0\|0.6\|1\|1\|5\|0\|295
Chr2	exon	71273352	71273573	71273352	ID=MsG0280010546.01.T01:exon:6771;Parent=MsG0280010546.01.T01
Chr2	exon	71274387	71274514	71274387	ID=MsG0280010546.01.T01:exon:6772;Parent=MsG0280010546.01.T01
Chr2	exon	71274734	71274792	71274734	ID=MsG0280010546.01.T01:exon:6773;Parent=MsG0280010546.01.T01
Chr2	exon	71275216	71275286	71275216	ID=MsG0280010546.01.T01:exon:6774;Parent=MsG0280010546.01.T01
Chr2	exon	71275361	71275768	71275361	ID=MsG0280010546.01.T01:exon:6775;Parent=MsG0280010546.01.T01
Chr2	CDS	71273352	71273573	71273352	ID=MsG0280010546.01.T01:cds;Parent=MsG0280010546.01.T01
Chr2	CDS	71274387	71274514	71274387	ID=MsG0280010546.01.T01:cds;Parent=MsG0280010546.01.T01
Chr2	CDS	71274734	71274792	71274734	ID=MsG0280010546.01.T01:cds;Parent=MsG0280010546.01.T01
Chr2	CDS	71275216	71275286	71275216	ID=MsG0280010546.01.T01:cds;Parent=MsG0280010546.01.T01
Chr2	CDS	71275361	71275768	71275361	ID=MsG0280010546.01.T01:cds;Parent=MsG0280010546.01.T01

Gene Sequence

>MsG0280010546.01.T01

ATGTCGAGGACTCAAAGAGTGGAGCGAATGAATCTTTGCAAATTGATCAAAAATGAGAAACGTGTCGAACATCATTTATCTTCAAGACATCATTCTTATGGAGAATGTAAGAGGTCAAGGGTAAGTGAGTACCCTCCTCCCATTTCAAGCATAGGGAGAACCGGAAAGCCTTGGGTTTATTTCAGATCTATTAGGAATAATGGAAGATTTGTCCTCGAAGAGGGAGAGGAAGGATTCTTTGAGAAGGAAAGGTGGGAATGGAGGAAGAGGCACTACTCTCGCTGCGAGACAAATTTAGTGGATGATGAAGTGAATTTTATTCAAGGAGGTGGCTTTGTGCGAGAGGGAAGGTTGGAGGTTTTAGAAGATTTGGAAGTTGCAAAAGATGTTACAAGGGGGGATGAGAGAGTGGGAGATTTCAATGCGGTGTGTCATGCGGATGAGAGGAGGGGGGTGAACTTGGTTACTCCGACGGCTATGTTCACTTGGTATCATGCGAGTGGTAATGCGATGAGTCGCATTGATAGAGCCCTTATCTCGGAGAGTTGGAGTAGTTGTTGGGGTGTGCCTTCTTTATGGGTCCTCCCTAGAACAATTTCGGACCATTGCCCATTGGTTCTTAAATATGGCAATTCGGATTGGGGTCCTAAACCTTTCCGGTTTAATAATTATTGGCTAGAAAACAAGTTGTTCAAGAAATTGGTGGAAGATGCGTGGTGTAATCAAAGGGTTAGTGGTTGGATGGCGGTGTTTGAAAACAAAATAAAAGGGTTAAAATTTGTAATTAGAGATTGGAGTAAAGAAGTGTATGGGAACCTTGATTCTAAAGTGGCTTGCTTAGTAGGGGAGATTGAGGATCTTGATGTCAAAGGGGAGTTAGGGGGTTGA

Protein sequence

>MsG0280010546.01.T01

MSRTQRVERMNLCKLIKNEKRVEHHLSSRHHSYGECKRSRVSEYPPPISSIGRTGKPWVYFRSIRNNGRFVLEEGEEGFFEKERWEWRKRHYSRCETNLVDDEVNFIQGGGFVREGRLEVLEDLEVAKDVTRGDERVGDFNAVCHADERRGVNLVTPTAMFTWYHASGNAMSRIDRALISESWSSCWGVPSLWVLPRTISDHCPLVLKYGNSDWGPKPFRFNNYWLENKLFKKLVEDAWCNQRVSGWMAVFENKIKGLKFVIRDWSKEVYGNLDSKVACLVGEIEDLDVKGELGG*