Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|
| Gene ID | Type | Classification |
|---|
| Gene ID | Type | Classification |
|---|
Co-expression Network
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|---|---|---|---|
| MsG0180000115.01 | MsG0280010576.01 | 0.825109 | 5.607932e-54 | 5.063891e-51 |
PPI
| Gene1 | Gene2 | Type |
|---|
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|
Find 18 sgRNAs with CRISPR-Local
Find 23 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| GAGATAATGGCCAGCTATTA+TGG | 0.169252 | 2:-71691333 | MsG0280010576.01.T01:CDS |
| TAGCTTAATTTCCTTCATTA+TGG | 0.231538 | 2:-71691452 | MsG0280010576.01.T01:CDS |
| GAAGATCTCGCTGGAGATAA+TGG | 0.357369 | 2:-71691346 | MsG0280010576.01.T01:CDS |
| TGTGCTAGCAGGCATGTAAA+TGG | 0.392327 | 2:+71691429 | None:intergenic |
| GTTTCTTGCAGGGAAACTAC+TGG | 0.441861 | 2:-71691693 | None:intergenic |
| AGATAATGGCCAGCTATTAT+GGG | 0.450219 | 2:-71691332 | MsG0280010576.01.T01:CDS |
| GATGCAGATTCACGGATTGA+TGG | 0.458907 | 2:-71691400 | MsG0280010576.01.T01:CDS |
| GAAACAGCATGCTGGATGCT+TGG | 0.462414 | 2:-71691671 | MsG0280010576.01.T01:CDS |
| CATGCTTTGATGCAGATTCA+CGG | 0.471288 | 2:-71691408 | MsG0280010576.01.T01:CDS |
| GCATCAAAGCATGTGCTAGC+AGG | 0.498922 | 2:+71691418 | None:intergenic |
| CATGTAAATGGCCATAATGA+AGG | 0.508714 | 2:+71691441 | None:intergenic |
| AAAATCACACCCATAATAGC+TGG | 0.538570 | 2:+71691323 | None:intergenic |
| AACTACTGGAAACAGCATGC+TGG | 0.545401 | 2:-71691679 | MsG0280010576.01.T01:CDS |
| GAATCTAGAATGGTTACTAA+AGG | 0.552638 | 2:+71691646 | None:intergenic |
| GACAAGAGGTGAATCTAGAA+TGG | 0.557132 | 2:+71691636 | None:intergenic |
| ATCGAATATGAAGATCTCGC+TGG | 0.595531 | 2:-71691355 | MsG0280010576.01.T01:CDS |
| TGGAATGAATGTTTCTTGCA+GGG | 0.607016 | 2:-71691703 | None:intergenic |
| TAGCAAAGTTCCTTAAAACA+AGG | 0.656653 | 2:-71691585 | MsG0280010576.01.T01:intron |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|---|---|---|---|---|
| !! | GAGTATAGTTAATTACAATT+AGG | + | Chr2:71691537-71691556 | None:intergenic | 20.0% |
| ! | TAGCTTAATTTCCTTCATTA+TGG | - | Chr2:71691563-71691582 | MsG0280010576.01.T01:intron | 25.0% |
| !!! | TTGTTCAATACCTTGTTTTA+AGG | + | Chr2:71691443-71691462 | None:intergenic | 25.0% |
| TAGCAAAGTTCCTTAAAACA+AGG | - | Chr2:71691430-71691449 | MsG0280010576.01.T01:CDS | 30.0% | |
| ! | GAATCTAGAATGGTTACTAA+AGG | + | Chr2:71691372-71691391 | None:intergenic | 30.0% |
| !!! | AAAAACCCCACGTTTTTTTA+TGG | - | Chr2:71691406-71691425 | MsG0280010576.01.T01:CDS | 30.0% |
| CATGTAAATGGCCATAATGA+AGG | + | Chr2:71691577-71691596 | None:intergenic | 35.0% | |
| TGCTAGCCATAAAAAAACGT+GGG | + | Chr2:71691415-71691434 | None:intergenic | 35.0% | |
| TTGCTAGCCATAAAAAAACG+TGG | + | Chr2:71691416-71691435 | None:intergenic | 35.0% | |
| ! | AGATAATGGCCAGCTATTAT+GGG | - | Chr2:71691683-71691702 | MsG0280010576.01.T01:CDS | 35.0% |
| ATCGAATATGAAGATCTCGC+TGG | - | Chr2:71691660-71691679 | MsG0280010576.01.T01:CDS | 40.0% | |
| CATGCTTTGATGCAGATTCA+CGG | - | Chr2:71691607-71691626 | MsG0280010576.01.T01:CDS | 40.0% | |
| GACAAGAGGTGAATCTAGAA+TGG | + | Chr2:71691382-71691401 | None:intergenic | 40.0% | |
| GCTAGCCATAAAAAAACGTG+GGG | + | Chr2:71691414-71691433 | None:intergenic | 40.0% | |
| ! | GAGATAATGGCCAGCTATTA+TGG | - | Chr2:71691682-71691701 | MsG0280010576.01.T01:CDS | 40.0% |
| ! | GCAAGCTATCACTTTGTTTG+TGG | - | Chr2:71691470-71691489 | MsG0280010576.01.T01:intron | 40.0% |
| ! | TGGGGTTTTTCATTGACAAG+AGG | + | Chr2:71691396-71691415 | None:intergenic | 40.0% |
| AACTACTGGAAACAGCATGC+TGG | - | Chr2:71691336-71691355 | MsG0280010576.01.T01:CDS | 45.0% | |
| GAAGATCTCGCTGGAGATAA+TGG | - | Chr2:71691669-71691688 | MsG0280010576.01.T01:CDS | 45.0% | |
| TGTGCTAGCAGGCATGTAAA+TGG | + | Chr2:71691589-71691608 | None:intergenic | 45.0% | |
| ! | GATGCAGATTCACGGATTGA+TGG | - | Chr2:71691615-71691634 | MsG0280010576.01.T01:CDS | 45.0% |
| GAAACAGCATGCTGGATGCT+TGG | - | Chr2:71691344-71691363 | MsG0280010576.01.T01:CDS | 50.0% | |
| GCATCAAAGCATGTGCTAGC+AGG | + | Chr2:71691600-71691619 | None:intergenic | 50.0% |
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| Chr2 | gene | 71691327 | 71691710 | 71691327 | ID=MsG0280010576.01;Name=MsG0280010576.01 |
| Chr2 | mRNA | 71691327 | 71691710 | 71691327 | ID=MsG0280010576.01.T01;Parent=MsG0280010576.01;Name=MsG0280010576.01.T01;_AED=0.48;_eAED=0.77;_QI=0|0|0|1|1|1|2|0|89 |
| Chr2 | exon | 71691586 | 71691710 | 71691586 | ID=MsG0280010576.01.T01:exon:6877;Parent=MsG0280010576.01.T01 |
| Chr2 | exon | 71691327 | 71691471 | 71691327 | ID=MsG0280010576.01.T01:exon:6876;Parent=MsG0280010576.01.T01 |
| Chr2 | CDS | 71691586 | 71691710 | 71691586 | ID=MsG0280010576.01.T01:cds;Parent=MsG0280010576.01.T01 |
| Chr2 | CDS | 71691327 | 71691471 | 71691327 | ID=MsG0280010576.01.T01:cds;Parent=MsG0280010576.01.T01 |
| Gene Sequence |
| Protein sequence |
