AlfalfaGEDB | MsG0280010668.01

M. sativa cultivar ZhongmuNo.1 / MsG0280010668.01

KEGG: -
GO: -
PFAM: PF12609

Nr:

Query id	Subject id	identity %	alignment length	mis matches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MsG0280010668.01.T01	XP_013465017.1	94.118	85	4	1	1	85	1	84	2.73E-49	160

Swissprot:

Query id	Subject id	identity %	alignment length	mis matches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score

Trembl:

Query id	Subject id	identity %	alignment length	mis matches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MsG0280010668.01.T01	A0A072VB82	94.118	85	4	1	1	85	1	84	1.30e-49	160

TFs/TRs:

Gene ID	Type	Classification

Protein Kinases:

Gene ID	Type	Classification

Network:

Co-expression Network

Gene1	Gene2	correlation coefficient	p_value	FDR
MsG0280010665.01	MsG0280010668.01	0.811161	8.041035e-51	4.941060e-48
MsG0280010668.01	MsG0780036060.01	0.800572	1.353597e-48	6.324118e-46

PPI

Gene1	Gene2	Type

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MsG0280010668.01.T01	MTR_2g089950	94.118	85	4	1	1	85	1	84	3.31e-53	160
MsG0280010668.01.T01	MTR_2g089925	90.588	85	7	1	1	85	1	84	6.64e-51	154
MsG0280010668.01.T01	MTR_2g089970	61.538	91	27	2	1	85	1	89	5.63e-33	109
MsG0280010668.01.T01	MTR_4g045990	64.835	91	24	3	1	85	1	89	6.51e-32	107
MsG0280010668.01.T01	MTR_2g089930	67.901	81	21	3	10	85	11	91	2.63e-31	105
MsG0280010668.01.T01	MTR_2g089945	67.901	81	21	3	10	85	11	91	4.91e-31	104
MsG0280010668.01.T01	MTR_2g089990	59.341	91	29	2	1	85	1	89	5.56e-31	104
MsG0280010668.01.T01	MTR_2g089905	55.319	94	31	2	1	85	1	92	3.14e-29	100
MsG0280010668.01.T01	MTR_2g089940	58.065	93	29	2	1	85	1	91	5.33e-28	97.1
MsG0280010668.01.T01	MTR_2g089985	58.065	93	29	2	1	85	1	91	5.33e-28	97.1
MsG0280010668.01.T01	MTR_2g089960	58.065	93	29	2	1	85	1	91	5.33e-28	97.1
MsG0280010668.01.T01	MTR_2g089965	53.061	98	31	2	1	85	1	96	3.52e-26	92.8
MsG0280010668.01.T01	MTR_2g089835	56.962	79	29	2	12	85	10	88	8.02e-23	84.0
MsG0280010668.01.T01	MTR_2g089865	62.821	78	24	3	9	81	10	87	1.05e-21	86.3
MsG0280010668.01.T01	MTR_2g481150	52.326	86	37	3	1	84	1	84	2.04e-21	80.5
MsG0280010668.01.T01	MTR_2g481160	53.488	86	36	3	1	84	1	84	2.84e-21	80.1
MsG0280010668.01.T01	MTR_2g081155	48.235	85	40	3	1	84	1	82	4.11e-19	74.3

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MsG0280010668.01.T01	AT4G10265	62.353	85	28	3	1	84	1	82	1.90e-29	100
MsG0280010668.01.T01	AT4G10270	55.435	92	32	3	1	85	1	90	4.68e-26	92.4

Find 23 sgRNAs with CRISPR-Local

Find 22 sgRNAs with CRISPR-GE

CRISPR-Local

CRISPR-Local
sgRNA_sequence	on_target_score	Position	Region
GGATTTCTCATCCCTCAATT+TGG	0.235218	2:-73141195	None:intergenic
CTGTCATGTTCTTGAGCTGC+TGG	0.343401	2:+73141243	MsG0280010668.01.T01:CDS
AAATGTTAATTTCTTTAGTT+GGG	0.374844	2:-73141269	None:intergenic
GAAATGTTAATTTCTTTAGT+TGG	0.379094	2:-73141270	None:intergenic
TGGAGTGGTGGAGGCTTTGA+AGG	0.421743	2:+73141067	MsG0280010668.01.T01:CDS
TTTCTTCTTCAACCAAATTG+AGG	0.427146	2:+73141183	MsG0280010668.01.T01:CDS
CAAAGATTCCTCCGACTGGC+TGG	0.435235	2:-73141216	None:intergenic
TTCTTCTTCAACCAAATTGA+GGG	0.442795	2:+73141184	MsG0280010668.01.T01:CDS
TGTCATGTTCTTGAGCTGCT+GGG	0.448867	2:+73141244	MsG0280010668.01.T01:CDS
AACATGCTAAACATAATATC+AGG	0.478609	2:+73141135	MsG0280010668.01.T01:CDS
AACAAGTGTTGGAGTGGTGG+AGG	0.510421	2:+73141058	MsG0280010668.01.T01:CDS
GTGGAGGCTTTGAAGGATCA+AGG	0.541801	2:+73141074	MsG0280010668.01.T01:CDS
TTCTCAAAGATTCCTCCGAC+TGG	0.550443	2:-73141220	None:intergenic
GTCATGTTCTTGAGCTGCTG+GGG	0.555931	2:+73141245	MsG0280010668.01.T01:CDS
CAAGAAGCCGAGCATGGACA+TGG	0.568226	2:+73141027	MsG0280010668.01.T01:CDS
CTACACACCATGTCCATGCT+CGG	0.576827	2:-73141034	None:intergenic
AGCAACAAGTGTTGGAGTGG+TGG	0.580152	2:+73141055	MsG0280010668.01.T01:CDS
TGAGTTCAAGAAGCCGAGCA+TGG	0.595479	2:+73141021	MsG0280010668.01.T01:CDS
AGGATCAAGGAATATGCAGA+TGG	0.620547	2:+73141087	MsG0280010668.01.T01:CDS
TGAGAAATCCAGCCAGTCGG+AGG	0.622085	2:+73141208	MsG0280010668.01.T01:CDS
TGGTGTGTAGCAACAAGTGT+TGG	0.630045	2:+73141047	MsG0280010668.01.T01:CDS
GGATGAGAAATCCAGCCAGT+CGG	0.660168	2:+73141205	MsG0280010668.01.T01:CDS
TGTAGCAACAAGTGTTGGAG+TGG	0.661959	2:+73141052	MsG0280010668.01.T01:CDS

CRISPR-GE

badsite warning	sgRNA_sequence	Strand	Position	Region	GC_content
!	AACATGCTAAACATAATATC+AGG	+	Chr2:73141135-73141154	MsG0280010668.01.T01:CDS	25.0%
!!!	TGAAGAAGAAATTTTGTTGT+TGG	-	Chr2:73141174-73141193	None:intergenic	25.0%
	TTCTTCTTCAACCAAATTGA+GGG	+	Chr2:73141184-73141203	MsG0280010668.01.T01:CDS	30.0%
	TTTCTTCTTCAACCAAATTG+AGG	+	Chr2:73141183-73141202	MsG0280010668.01.T01:CDS	30.0%
	AGGATCAAGGAATATGCAGA+TGG	+	Chr2:73141087-73141106	MsG0280010668.01.T01:CDS	40.0%
	GGATTTCTCATCCCTCAATT+TGG	-	Chr2:73141198-73141217	None:intergenic	40.0%
	TGGTGTGTAGCAACAAGTGT+TGG	+	Chr2:73141047-73141066	MsG0280010668.01.T01:CDS	45.0%
	TGTCATGTTCTTGAGCTGCT+GGG	+	Chr2:73141244-73141263	MsG0280010668.01.T01:CDS	45.0%
	TTCTCAAAGATTCCTCCGAC+TGG	-	Chr2:73141223-73141242	None:intergenic	45.0%
!!	TGTAGCAACAAGTGTTGGAG+TGG	+	Chr2:73141052-73141071	MsG0280010668.01.T01:CDS	45.0%
	CTACACACCATGTCCATGCT+CGG	-	Chr2:73141037-73141056	None:intergenic	50.0%
	CTGTCATGTTCTTGAGCTGC+TGG	+	Chr2:73141243-73141262	MsG0280010668.01.T01:CDS	50.0%
	GGATGAGAAATCCAGCCAGT+CGG	+	Chr2:73141205-73141224	MsG0280010668.01.T01:CDS	50.0%
	GTCATGTTCTTGAGCTGCTG+GGG	+	Chr2:73141245-73141264	MsG0280010668.01.T01:CDS	50.0%
	TGAGTTCAAGAAGCCGAGCA+TGG	+	Chr2:73141021-73141040	MsG0280010668.01.T01:CDS	50.0%
!!	AACAAGTGTTGGAGTGGTGG+AGG	+	Chr2:73141058-73141077	MsG0280010668.01.T01:CDS	50.0%
!!	AGCAACAAGTGTTGGAGTGG+TGG	+	Chr2:73141055-73141074	MsG0280010668.01.T01:CDS	50.0%
!!	GTGGAGGCTTTGAAGGATCA+AGG	+	Chr2:73141074-73141093	MsG0280010668.01.T01:CDS	50.0%
	CAAAGATTCCTCCGACTGGC+TGG	-	Chr2:73141219-73141238	None:intergenic	55.0%
	CAAGAAGCCGAGCATGGACA+TGG	+	Chr2:73141027-73141046	MsG0280010668.01.T01:CDS	55.0%
	TGAGAAATCCAGCCAGTCGG+AGG	+	Chr2:73141208-73141227	MsG0280010668.01.T01:CDS	55.0%
!	TGGAGTGGTGGAGGCTTTGA+AGG	+	Chr2:73141067-73141086	MsG0280010668.01.T01:CDS	55.0%

Chromosome	Type	Strat	End	Strand	Name
Chr2	gene	73141020	73141277	73141020	ID=MsG0280010668.01;Name=MsG0280010668.01
Chr2	mRNA	73141020	73141277	73141020	ID=MsG0280010668.01.T01;Parent=MsG0280010668.01;Name=MsG0280010668.01.T01;_AED=0.50;_eAED=0.50;_QI=0\|-1\|0\|1\|-1\|0\|1\|0\|85
Chr2	exon	73141020	73141277	73141020	ID=MsG0280010668.01.T01:exon:19140;Parent=MsG0280010668.01.T01
Chr2	CDS	73141020	73141277	73141020	ID=MsG0280010668.01.T01:cds;Parent=MsG0280010668.01.T01
Chr2	mRNA	73141040	73141144	73141040	ID=MsG0280010668.01.T02;Parent=MsG0280010668.01;Name=MsG0280010668.01.T02;_AED=0.50;_eAED=0.50;_QI=0\|-1\|0\|1\|-1\|0\|1\|0\|34
Chr2	exon	73141040	73141144	73141040	ID=MsG0280010668.01.T02:exon:19141;Parent=MsG0280010668.01.T02
Chr2	CDS	73141040	73141144	73141040	ID=MsG0280010668.01.T02:cds;Parent=MsG0280010668.01.T02
Chr2	mRNA	73141138	73141203	73141138	ID=MsG0280010668.01.T03;Parent=MsG0280010668.01;Name=MsG0280010668.01.T03;_AED=0.50;_eAED=0.50;_QI=0\|-1\|0\|1\|-1\|0\|1\|0\|21
Chr2	exon	73141138	73141203	73141138	ID=MsG0280010668.01.T03:exon:19142;Parent=MsG0280010668.01.T03
Chr2	CDS	73141138	73141203	73141138	ID=MsG0280010668.01.T03:cds;Parent=MsG0280010668.01.T03

Gene Sequence

>MsG0280010668.01.T02

ATGGACATGGTGTGTAGCAACAAGTGTTGGAGTGGTGGAGGCTTTGAAGGATCAAGGAATATGCAGATGGAACAGTGTTATAAGATCAGCACAACAACATGCTAA

>MsG0280010668.01.T03

ATGCTAAACATAATATCAGGTCTTTGTCTCATGCCAACAACAAAATTTCTTCTTCAACCAAATTGA

>MsG0280010668.01.T01

ATGAGTTCAAGAAGCCGAGCATGGACATGGTGTGTAGCAACAAGTGTTGGAGTGGTGGAGGCTTTGAAGGATCAAGGAATATGCAGATGGAACAGTGTTATAAGATCAGCACAACAACATGCTAAACATAATATCAGGTCTTTGTCTCATGCCAACAACAAAATTTCTTCTTCAACCAAATTGAGGGATGAGAAATCCAGCCAGTCGGAGGAATCTTTGAGAACTGTCATGTTCTTGAGCTGCTGGGGTCCCAACTAA

Protein sequence

>MsG0280010668.01.T02

MDMVCSNKCWSGGGFEGSRNMQMEQCYKISTTTC*

>MsG0280010668.01.T03

MLNIISGLCLMPTTKFLLQPN*

>MsG0280010668.01.T01

MSSRSRAWTWCVATSVGVVEALKDQGICRWNSVIRSAQQHAKHNIRSLSHANNKISSSTKLRDEKSSQSEESLRTVMFLSCWGPN*