Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MsG0780036060.01.T01 | XP_013447443.1 | 97.674 | 301 | 7 | 0 | 1 | 301 | 1 | 301 | 0 | 600 |
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MsG0780036060.01.T01 | Q9M886 | 60.351 | 285 | 69 | 4 | 1 | 281 | 1 | 245 | 2.22E-112 | 328 |
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MsG0780036060.01.T01 | A0A072TVI9 | 97.674 | 301 | 7 | 0 | 1 | 301 | 1 | 301 | 0.0 | 600 |
| Gene ID | Type | Classification |
|---|---|---|
| MsG0780036060.01.T01 | TF | LOB |
| Gene ID | Type | Classification |
|---|
Co-expression Network
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|---|---|---|---|
| MsG0180000466.01 | MsG0780036060.01 | 0.803885 | 2.815696e-49 | 1.431356e-46 |
| MsG0180001034.01 | MsG0780036060.01 | 0.824615 | 7.334214e-54 | 6.530435e-51 |
| MsG0180003430.01 | MsG0780036060.01 | 0.817603 | 3.026958e-52 | 2.214329e-49 |
| MsG0180004830.01 | MsG0780036060.01 | 0.812253 | 4.653616e-51 | 2.944248e-48 |
| MsG0180005338.01 | MsG0780036060.01 | -0.811725 | 6.065141e-51 | 3.782919e-48 |
| MsG0180005898.01 | MsG0780036060.01 | -0.820514 | 6.590670e-53 | 5.225552e-50 |
| MsG0180006010.01 | MsG0780036060.01 | 0.836295 | 1.017552e-56 | 1.277040e-53 |
| MsG0180006183.01 | MsG0780036060.01 | 0.813735 | 2.201634e-51 | 1.449424e-48 |
| MsG0180006253.01 | MsG0780036060.01 | 0.821390 | 4.141628e-53 | 3.364983e-50 |
| MsG0280006319.01 | MsG0780036060.01 | -0.807468 | 4.980890e-50 | 2.776567e-47 |
| MsG0280006402.01 | MsG0780036060.01 | -0.830051 | 3.649902e-55 | 3.802589e-52 |
| MsG0280010668.01 | MsG0780036060.01 | 0.800572 | 1.353597e-48 | 6.324118e-46 |
| MsG0280010673.01 | MsG0780036060.01 | 0.805430 | 1.339888e-49 | 7.087295e-47 |
| MsG0280011362.01 | MsG0780036060.01 | -0.808935 | 2.424831e-50 | 1.404620e-47 |
| MsG0680035570.01 | MsG0780036060.01 | 0.818370 | 2.030966e-52 | 1.517528e-49 |
| MsG0480020797.01 | MsG0780036060.01 | 0.800681 | 1.285932e-48 | 6.024528e-46 |
| MsG0480021151.01 | MsG0780036060.01 | -0.804260 | 2.352446e-49 | 1.207353e-46 |
| MsG0480023466.01 | MsG0780036060.01 | -0.808389 | 3.172333e-50 | 1.811614e-47 |
| MsG0580024471.01 | MsG0780036060.01 | -0.815977 | 7.009794e-52 | 4.904826e-49 |
| MsG0380015349.01 | MsG0780036060.01 | 0.818452 | 1.946074e-52 | 1.457421e-49 |
| MsG0380015393.01 | MsG0780036060.01 | 0.823517 | 1.327176e-53 | 1.145311e-50 |
| MsG0380016031.01 | MsG0780036060.01 | 0.818545 | 1.853668e-52 | 1.391669e-49 |
| MsG0380016066.01 | MsG0780036060.01 | -0.820560 | 6.430306e-53 | 5.105120e-50 |
| MsG0380016151.01 | MsG0780036060.01 | -0.801703 | 7.943400e-49 | 3.818864e-46 |
| MsG0380016936.01 | MsG0780036060.01 | -0.820599 | 6.298920e-53 | 5.005885e-50 |
| MsG0380017380.01 | MsG0780036060.01 | 0.801188 | 1.013218e-48 | 4.808354e-46 |
| MsG0380017666.01 | MsG0780036060.01 | 0.827971 | 1.165116e-54 | 1.142466e-51 |
| MsG0580027732.01 | MsG0780036060.01 | -0.810982 | 8.791280e-51 | 5.377452e-48 |
| MsG0280007423.01 | MsG0780036060.01 | -0.834131 | 3.579141e-56 | 4.208150e-53 |
| MsG0280007874.01 | MsG0780036060.01 | 0.803668 | 3.122965e-49 | 1.578620e-46 |
| MsG0680035665.01 | MsG0780036060.01 | -0.817055 | 4.021043e-52 | 2.897867e-49 |
| MsG0780036060.01 | MsG0780038919.01 | 0.835179 | 1.951153e-56 | 2.367440e-53 |
| MsG0780036060.01 | MsG0780039805.01 | -0.816127 | 6.490951e-52 | 4.560378e-49 |
| MsG0780036060.01 | MsG0780040847.01 | 0.811861 | 5.664408e-51 | 3.545669e-48 |
| MsG0780036060.01 | MsG0780041403.01 | 0.826166 | 3.149033e-54 | 2.930390e-51 |
| MsG0780036060.01 | MsG0780041823.01 | 0.824295 | 8.720428e-54 | 7.692091e-51 |
| MsG0780036060.01 | MsG0880043495.01 | 0.803717 | 3.050512e-49 | 1.543948e-46 |
| MsG0780036060.01 | MsG0880044317.01 | -0.827967 | 1.167557e-54 | 1.144720e-51 |
| MsG0780036060.01 | MsG0880044318.01 | -0.822671 | 2.091047e-53 | 1.761899e-50 |
| MsG0780036060.01 | MsG0880044453.01 | -0.806118 | 9.607492e-50 | 5.172367e-47 |
| MsG0780036060.01 | MsG0880044834.01 | -0.832730 | 7.999818e-56 | 9.019918e-53 |
| MsG0780036060.01 | MsG0880045725.01 | 0.803531 | 3.334504e-49 | 1.679768e-46 |
| MsG0780036060.01 | MsG0880045761.01 | -0.835664 | 1.471198e-56 | 1.811230e-53 |
| MsG0780036060.01 | MsG0880046324.01 | -0.816332 | 5.841134e-52 | 4.127126e-49 |
| MsG0780036060.01 | MsG0880047090.01 | 0.822782 | 1.970467e-53 | 1.665519e-50 |
| MsG0780036060.01 | MsG0880047207.01 | -0.804459 | 2.138259e-49 | 1.103075e-46 |
PPI
| Gene1 | Gene2 | Type |
|---|
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MsG0780036060.01.T01 | MTR_7g007010 | 97.674 | 301 | 7 | 0 | 1 | 301 | 1 | 301 | 0.0 | 600 |
| MsG0780036060.01.T01 | MTR_6g018270 | 60.000 | 310 | 83 | 9 | 1 | 301 | 1 | 278 | 3.35e-112 | 326 |
| MsG0780036060.01.T01 | MTR_6g453130 | 44.395 | 223 | 114 | 4 | 1 | 213 | 1 | 223 | 9.89e-58 | 185 |
| MsG0780036060.01.T01 | MTR_4g095600 | 61.417 | 127 | 46 | 1 | 3 | 129 | 1 | 124 | 1.87e-54 | 177 |
| MsG0780036060.01.T01 | MTR_1g110840 | 45.673 | 208 | 99 | 5 | 1 | 196 | 1 | 206 | 2.77e-53 | 174 |
| MsG0780036060.01.T01 | MTR_6g054150 | 51.149 | 174 | 68 | 6 | 1 | 164 | 1 | 167 | 1.43e-52 | 172 |
| MsG0780036060.01.T01 | MTR_5g015880 | 64.545 | 110 | 39 | 0 | 3 | 112 | 1 | 110 | 6.98e-52 | 170 |
| MsG0780036060.01.T01 | MTR_6g054080 | 50.575 | 174 | 69 | 6 | 1 | 164 | 1 | 167 | 2.23e-51 | 169 |
| MsG0780036060.01.T01 | MTR_1g106420 | 60.577 | 104 | 41 | 0 | 3 | 106 | 1 | 104 | 9.37e-45 | 150 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MsG0780036060.01.T01 | AT3G02550 | 60.351 | 285 | 69 | 4 | 1 | 281 | 1 | 245 | 2.26e-113 | 328 |
| MsG0780036060.01.T01 | AT1G67100 | 82.270 | 141 | 22 | 2 | 1 | 138 | 1 | 141 | 1.34e-82 | 249 |
| MsG0780036060.01.T01 | AT1G68510 | 62.941 | 170 | 53 | 2 | 1 | 160 | 1 | 170 | 3.82e-74 | 228 |
| MsG0780036060.01.T01 | AT3G49940 | 70.000 | 110 | 33 | 0 | 3 | 112 | 1 | 110 | 3.58e-55 | 179 |
| MsG0780036060.01.T01 | AT5G67420 | 65.455 | 110 | 38 | 0 | 3 | 112 | 1 | 110 | 5.66e-54 | 176 |
| MsG0780036060.01.T01 | AT5G67420 | 65.455 | 110 | 38 | 0 | 3 | 112 | 1 | 110 | 5.66e-54 | 176 |
| MsG0780036060.01.T01 | AT4G37540 | 66.667 | 111 | 37 | 0 | 3 | 113 | 1 | 111 | 3.85e-53 | 174 |
Find 107 sgRNAs with CRISPR-Local
Find 141 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| GTGGCATTAGCTTGTGATTC+AGG | 0.204656 | 7:+1954132 | None:intergenic |
| AAACCGACTGAACCGTAAAT+TGG | 0.257065 | 7:+1953710 | None:intergenic |
| GGCTCATGGAATGTGGAGTT+GGG | 0.290152 | 7:-1953083 | MsG0780036060.01.T01:CDS |
| GACCCGGTTTGGTTCGGTTT+CGG | 0.302359 | 7:-1953042 | MsG0780036060.01.T01:three_prime_UTR |
| ACTTTCTCATCTACATAGTT+TGG | 0.307581 | 7:+1952857 | None:intergenic |
| ACCAGCACGACCGTAGAATT+TGG | 0.324902 | 7:+1954098 | None:intergenic |
| GCAGCTGCTTCCGAAGTTAT+TGG | 0.331265 | 7:+1953617 | None:intergenic |
| TTGACATAGCTGCCAGCTTC+CGG | 0.350615 | 7:+1953679 | None:intergenic |
| GTCTGAGGCGGTTGCGGTGG+TGG | 0.368975 | 7:-1953321 | MsG0780036060.01.T01:CDS |
| CTCTCACCGGTTCGGTCAAC+CGG | 0.370315 | 7:+1953218 | None:intergenic |
| CGAATAGTGAATCCAATTTA+CGG | 0.370383 | 7:-1953722 | MsG0780036060.01.T01:CDS |
| TGTAGATGAGAAAGTACATT+TGG | 0.383973 | 7:-1952849 | MsG0780036060.01.T01:three_prime_UTR |
| CTCTTCTTGTATCTTCGGCT+TGG | 0.390746 | 7:+1953466 | None:intergenic |
| GGAGTTGGGGCTTCAATATC+CGG | 0.399627 | 7:-1953069 | MsG0780036060.01.T01:CDS |
| GAAAAGAAGGGTTGTATTGA+AGG | 0.400262 | 7:-1953117 | MsG0780036060.01.T01:CDS |
| ACGTGATAAACGCGTGAAAC+CGG | 0.400688 | 7:+1953152 | None:intergenic |
| CGAGACATGACGGATGTCAT+AGG | 0.402077 | 7:+1953568 | None:intergenic |
| TGGCTCATGGAATGTGGAGT+TGG | 0.402516 | 7:-1953084 | MsG0780036060.01.T01:CDS |
| CCTCTCCACTCTCACCGGTT+CGG | 0.407185 | 7:+1953210 | None:intergenic |
| GATCTTATTTAGAGATGAAC+CGG | 0.409879 | 7:-1953261 | MsG0780036060.01.T01:CDS |
| TATCCGGCTTAGACCCGGTT+TGG | 0.419234 | 7:-1953053 | MsG0780036060.01.T01:exon |
| GGCTTAGACCCGGTTTGGTT+CGG | 0.420361 | 7:-1953048 | MsG0780036060.01.T01:exon |
| CGGTTCATCACCTCCTCAAC+CGG | 0.421068 | 7:+1953377 | None:intergenic |
| CCACCAGTCTGAGGCGGTTG+CGG | 0.421397 | 7:-1953327 | MsG0780036060.01.T01:CDS |
| GTTGGTTTAGAGCTGACTCT+TGG | 0.426866 | 7:-1953182 | MsG0780036060.01.T01:CDS |
| GTTTATTATGGTCCGGAAGC+TGG | 0.428216 | 7:-1953691 | MsG0780036060.01.T01:CDS |
| TTGGTTTAGAGCTGACTCTT+GGG | 0.430742 | 7:-1953181 | MsG0780036060.01.T01:CDS |
| ACCGGTCGGCTCAGTTGAAC+CGG | 0.430775 | 7:+1953409 | None:intergenic |
| GAGGCGGTTGCGGTGGTGGA+TGG | 0.434659 | 7:-1953317 | MsG0780036060.01.T01:CDS |
| GAACCGAAACCGAACCAAAC+CGG | 0.434738 | 7:+1953039 | None:intergenic |
| ACGGTTCAGTCGGTTTATTA+TGG | 0.442488 | 7:-1953703 | MsG0780036060.01.T01:CDS |
| GCCGACCGGTTCGGTTGAAC+CGG | 0.442999 | 7:-1953396 | MsG0780036060.01.T01:CDS |
| CGGTGAGAGTGGAGAGGAAA+AGG | 0.443712 | 7:-1953204 | MsG0780036060.01.T01:CDS |
| CGAACCTGGACGAAGATGTT+CGG | 0.443982 | 7:+1954050 | None:intergenic |
| TGGCATTAGCTTGTGATTCA+GGG | 0.445704 | 7:+1954133 | None:intergenic |
| GAACCTGGACGAAGATGTTC+GGG | 0.446611 | 7:+1954051 | None:intergenic |
| GCTGACTCTTGGGCTTGAAC+CGG | 0.449902 | 7:-1953171 | MsG0780036060.01.T01:CDS |
| GTATTGAAGGATTGTGGTGT+TGG | 0.451012 | 7:-1953104 | MsG0780036060.01.T01:CDS |
| CTTCGGAAGCAGCTGCTAAC+GGG | 0.456571 | 7:-1953610 | MsG0780036060.01.T01:CDS |
| GCCAAATTCTACGGTCGTGC+TGG | 0.457716 | 7:-1954099 | MsG0780036060.01.T01:CDS |
| GAAGCAGCTGCTAACGGGAG+GGG | 0.464479 | 7:-1953605 | MsG0780036060.01.T01:CDS |
| GTGTTTCTCGCCAAATTCTA+CGG | 0.467341 | 7:-1954108 | MsG0780036060.01.T01:CDS |
| TTCAATATCCGGCTTAGACC+CGG | 0.468946 | 7:-1953058 | MsG0780036060.01.T01:exon |
| TTCAACTGAGCCGACCGGTT+CGG | 0.472535 | 7:-1953405 | MsG0780036060.01.T01:CDS |
| GAGAGTGGAGAGGAAAAGGT+TGG | 0.474231 | 7:-1953200 | MsG0780036060.01.T01:CDS |
| GAGAACAAGGGATTTGAATC+CGG | 0.474733 | 7:-1953428 | MsG0780036060.01.T01:CDS |
| ATGAAGCCGGTTGACCGAAC+CGG | 0.475639 | 7:-1953224 | MsG0780036060.01.T01:CDS |
| CTCAACCGGTTCAACCGAAC+CGG | 0.476475 | 7:+1953391 | None:intergenic |
| ATTTAGAGATGAACCGGAGT+TGG | 0.489333 | 7:-1953255 | MsG0780036060.01.T01:CDS |
| CGGTTCGGTTGAACCGGTTG+AGG | 0.489995 | 7:-1953390 | MsG0780036060.01.T01:CDS |
| AAAGAGTAGTAATGAGAACA+AGG | 0.495367 | 7:-1953441 | MsG0780036060.01.T01:CDS |
| TGGTTCCTGTGAAGAAAAGA+AGG | 0.501528 | 7:-1953130 | MsG0780036060.01.T01:CDS |
| TCCGAAGTTATTGGTGTGAT+CGG | 0.501864 | 7:+1953626 | None:intergenic |
| ATGTCATAGGCTTTGAGTGG+TGG | 0.508004 | 7:+1953581 | None:intergenic |
| GAGATGAACCGGAGTTGGAC+CGG | 0.508299 | 7:-1953250 | MsG0780036060.01.T01:CDS |
| CCACCGCAACCGCCTCAGAC+TGG | 0.514674 | 7:+1953324 | None:intergenic |
| AGGATTGTGGTGTTGGCTCA+TGG | 0.516288 | 7:-1953097 | MsG0780036060.01.T01:CDS |
| AACCGAAACCGAACCAAACC+GGG | 0.520305 | 7:+1953040 | None:intergenic |
| GTTCGGGACCGGCGTTAACA+AGG | 0.520425 | 7:+1954067 | None:intergenic |
| GGAAAACTCGGCCGCGTCTA+CGG | 0.520701 | 7:-1953540 | MsG0780036060.01.T01:CDS |
| CCAGTCTGAGGCGGTTGCGG+TGG | 0.522968 | 7:-1953324 | MsG0780036060.01.T01:CDS |
| CGGTTGAGGAGGTGATGAAC+CGG | 0.528263 | 7:-1953376 | MsG0780036060.01.T01:CDS |
| GAACCAAACCGGGTCTAAGC+CGG | 0.534041 | 7:+1953050 | None:intergenic |
| GTTGACCGAACCGGTGAGAG+TGG | 0.534361 | 7:-1953215 | MsG0780036060.01.T01:CDS |
| CATCCGTCATGTCTCGAAAG+AGG | 0.536392 | 7:-1953561 | MsG0780036060.01.T01:CDS |
| ACTTCGGAAGCAGCTGCTAA+CGG | 0.537457 | 7:-1953611 | MsG0780036060.01.T01:CDS |
| TGTCATAGGCTTTGAGTGGT+GGG | 0.543552 | 7:+1953582 | None:intergenic |
| GGTTGTAGAGTACTAAGAAA+AGG | 0.544678 | 7:-1954204 | MsG0780036060.01.T01:CDS |
| GCTCATGGAATGTGGAGTTG+GGG | 0.544869 | 7:-1953082 | MsG0780036060.01.T01:CDS |
| AAAATATGGGAAAGAAAGAA+GGG | 0.546091 | 7:+1954339 | None:intergenic |
| TTCGGTTGAACCGGTTGAGG+AGG | 0.552024 | 7:-1953387 | MsG0780036060.01.T01:CDS |
| GGTCCCGAACATCTTCGTCC+AGG | 0.558956 | 7:-1954054 | MsG0780036060.01.T01:intron |
| CGGAAGCAGCTGCTAACGGG+AGG | 0.561761 | 7:-1953607 | MsG0780036060.01.T01:CDS |
| TCAGTCGGTTTATTATGGTC+CGG | 0.563725 | 7:-1953698 | MsG0780036060.01.T01:CDS |
| TGGACGAAGATGTTCGGGAC+CGG | 0.564250 | 7:+1954056 | None:intergenic |
| TCTAGTACCTGAAAATCCAT+TGG | 0.565036 | 7:-1954411 | MsG0780036060.01.T01:five_prime_UTR |
| GGAAGCAGCTGCTAACGGGA+GGG | 0.580906 | 7:-1953606 | MsG0780036060.01.T01:CDS |
| AATCCAATTTACGGTTCAGT+CGG | 0.582091 | 7:-1953713 | MsG0780036060.01.T01:CDS |
| TGGTGTTGGCTCATGGAATG+TGG | 0.583661 | 7:-1953090 | MsG0780036060.01.T01:CDS |
| TCCGGTTCAACTGAGCCGAC+CGG | 0.592967 | 7:-1953410 | MsG0780036060.01.T01:CDS |
| GGTTTCACGCGTTTATCACG+TGG | 0.593523 | 7:-1953150 | MsG0780036060.01.T01:CDS |
| CTCAACAGCGAGTTAAGACT+CGG | 0.596868 | 7:-1953511 | MsG0780036060.01.T01:CDS |
| TTTCCTCTTTCGAGACATGA+CGG | 0.597148 | 7:+1953558 | None:intergenic |
| CTGGCAGCTATGTCAAGCCG+CGG | 0.599587 | 7:-1953672 | MsG0780036060.01.T01:CDS |
| GCAGCTATGTCAAGCCGCGG+TGG | 0.604170 | 7:-1953669 | MsG0780036060.01.T01:CDS |
| AGGGTTGTATTGAAGGATTG+TGG | 0.614044 | 7:-1953110 | MsG0780036060.01.T01:CDS |
| AAAATGCGGATGAGTTGTAA+CGG | 0.616520 | 7:-1954225 | MsG0780036060.01.T01:CDS |
| GCCGATCACACCAATAACTT+CGG | 0.617983 | 7:-1953627 | MsG0780036060.01.T01:CDS |
| GTCGATTAGCCACCAGTCTG+AGG | 0.618436 | 7:-1953336 | MsG0780036060.01.T01:CDS |
| GTAGAATTTGGCGAGAAACA+CGG | 0.619114 | 7:+1954110 | None:intergenic |
| TTAAGACTCGGTCGCGTAAG+CGG | 0.620497 | 7:-1953499 | MsG0780036060.01.T01:CDS |
| CAAGCCGAAGATACAAGAAG+AGG | 0.623975 | 7:-1953465 | MsG0780036060.01.T01:CDS |
| GTTGGACCGGAAAATGAAGC+CGG | 0.627386 | 7:-1953237 | MsG0780036060.01.T01:CDS |
| CCGCAACCGCCTCAGACTGG+TGG | 0.630942 | 7:+1953327 | None:intergenic |
| GCATAAGACCTTGTTTACAG+TGG | 0.631728 | 7:-1954164 | MsG0780036060.01.T01:CDS |
| AAGAGTAGTAATGAGAACAA+GGG | 0.634462 | 7:-1953440 | MsG0780036060.01.T01:CDS |
| ACCGGTTCAACCGAACCGGT+CGG | 0.636400 | 7:+1953395 | None:intergenic |
| ACGACTCATGACTAGCTGAC+CGG | 0.638954 | 7:+1953357 | None:intergenic |
| TGTCTCGAAAGAGGAAAACT+CGG | 0.644691 | 7:-1953552 | MsG0780036060.01.T01:CDS |
| CTCATGAACCTTGTTAACGC+CGG | 0.654635 | 7:-1954075 | MsG0780036060.01.T01:CDS |
| TTGAGTTAACTCCGTAGACG+CGG | 0.656912 | 7:+1953529 | None:intergenic |
| TCGTTGTTGTACGAAGCATG+TGG | 0.660850 | 7:-1953746 | MsG0780036060.01.T01:CDS |
| CCGAACCGGTGAGAGTGGAG+AGG | 0.672740 | 7:-1953210 | MsG0780036060.01.T01:CDS |
| GGTTCCTGTGAAGAAAAGAA+GGG | 0.688577 | 7:-1953129 | MsG0780036060.01.T01:CDS |
| GATTAGCCACCAGTCTGAGG+CGG | 0.698548 | 7:-1953333 | MsG0780036060.01.T01:CDS |
| CGGATGTCATAGGCTTTGAG+TGG | 0.702654 | 7:+1953578 | None:intergenic |
| GAATTTGGCGAGAAACACGG+TGG | 0.783343 | 7:+1954113 | None:intergenic |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|---|---|---|---|---|
| !!! | AAATCTTTTATTTTTTCAAT+TGG | - | Chr7:1952982-1953001 | MsG0780036060.01.T01:three_prime_UTR | 10.0% |
| !!! | AATCTTTTATTTTTTCAATT+GGG | - | Chr7:1952983-1953002 | MsG0780036060.01.T01:three_prime_UTR | 10.0% |
| !!! | TTATATATGTAATTTTGTTA+AGG | + | Chr7:1953284-1953303 | None:intergenic | 10.0% |
| !! | AACAACAAAAAGTTATTAAA+AGG | + | Chr7:1954321-1954340 | None:intergenic | 15.0% |
| !! | ATTAAAAACTAAACAAGAAA+CGG | + | Chr7:1954293-1954312 | None:intergenic | 15.0% |
| !!! | GAAAATTTTCATTAAAAATG+CGG | - | Chr7:1953011-1953030 | MsG0780036060.01.T01:three_prime_UTR | 15.0% |
| !! | ATTGTGTCTATGAAAAATAT+GGG | + | Chr7:1952927-1952946 | None:intergenic | 20.0% |
| !! | GAAAAATTAGACTTTGATAT+AGG | + | Chr7:1952956-1952975 | None:intergenic | 20.0% |
| !!! | ACAAATATATCAACTTTTGT+TGG | + | Chr7:1953373-1953392 | None:intergenic | 20.0% |
| !!! | ACTTTTTGTTGTTTACATTT+TGG | - | Chr7:1954328-1954347 | MsG0780036060.01.T01:five_prime_UTR | 20.0% |
| !!! | CTTTTTGTTGTTTACATTTT+GGG | - | Chr7:1954329-1954348 | MsG0780036060.01.T01:five_prime_UTR | 20.0% |
| ! | AAAAATATGGGAAAGAAAGA+AGG | + | Chr7:1952915-1952934 | None:intergenic | 25.0% |
| ! | AAAATATGGGAAAGAAAGAA+GGG | + | Chr7:1952914-1952933 | None:intergenic | 25.0% |
| ! | ATCTCTCTATAAGAAAAAGT+TGG | - | Chr7:1953346-1953365 | MsG0780036060.01.T01:CDS | 25.0% |
| ! | GATTGTGTCTATGAAAAATA+TGG | + | Chr7:1952928-1952947 | None:intergenic | 25.0% |
| AAAGAGTAGTAATGAGAACA+AGG | - | Chr7:1953809-1953828 | MsG0780036060.01.T01:intron | 30.0% | |
| AAGAGTAGTAATGAGAACAA+GGG | - | Chr7:1953810-1953829 | MsG0780036060.01.T01:intron | 30.0% | |
| ACTTTCTCATCTACATAGTT+TGG | + | Chr7:1954396-1954415 | None:intergenic | 30.0% | |
| CGAATAGTGAATCCAATTTA+CGG | - | Chr7:1953528-1953547 | MsG0780036060.01.T01:CDS | 30.0% | |
| GTATTGCATGTTTGGATAAA+CGG | + | Chr7:1953322-1953341 | None:intergenic | 30.0% | |
| ! | GATCTTATTTAGAGATGAAC+CGG | - | Chr7:1953989-1954008 | MsG0780036060.01.T01:intron | 30.0% |
| ! | TGTAGATGAGAAAGTACATT+TGG | - | Chr7:1954401-1954420 | MsG0780036060.01.T01:five_prime_UTR | 30.0% |
| ! | TTTTCCTCTTCTTGTATCTT+CGG | + | Chr7:1953792-1953811 | None:intergenic | 30.0% |
| !!! | ATTTTCAGGTACTAGATTGT+GGG | + | Chr7:1952835-1952854 | None:intergenic | 30.0% |
| AAAATGCGGATGAGTTGTAA+CGG | - | Chr7:1953025-1953044 | MsG0780036060.01.T01:three_prime_UTR | 35.0% | |
| AATCCAATTTACGGTTCAGT+CGG | - | Chr7:1953537-1953556 | MsG0780036060.01.T01:CDS | 35.0% | |
| AGATGTGTGTATTGCATGTT+TGG | + | Chr7:1953330-1953349 | None:intergenic | 35.0% | |
| CATGCACTGAAACTGTAAAA+TGG | + | Chr7:1953245-1953264 | None:intergenic | 35.0% | |
| GAAAAGAAGGGTTGTATTGA+AGG | - | Chr7:1954133-1954152 | MsG0780036060.01.T01:CDS | 35.0% | |
| TCTAGTACCTGAAAATCCAT+TGG | - | Chr7:1952839-1952858 | MsG0780036060.01.T01:three_prime_UTR | 35.0% | |
| ! | CTTTTGATCCACTGTAAACA+AGG | + | Chr7:1953097-1953116 | None:intergenic | 35.0% |
| ! | GGTTGTAGAGTACTAAGAAA+AGG | - | Chr7:1953046-1953065 | MsG0780036060.01.T01:exon | 35.0% |
| !!! | AATGTCACTTTTTACGAACC+TGG | + | Chr7:1953217-1953236 | None:intergenic | 35.0% |
| !!! | GATTTTCAGGTACTAGATTG+TGG | + | Chr7:1952836-1952855 | None:intergenic | 35.0% |
| !!! | TTTTCAGGTACTAGATTGTG+GGG | + | Chr7:1952834-1952853 | None:intergenic | 35.0% |
| AAACCGACTGAACCGTAAAT+TGG | + | Chr7:1953543-1953562 | None:intergenic | 40.0% | |
| AGGGTTGTATTGAAGGATTG+TGG | - | Chr7:1954140-1954159 | MsG0780036060.01.T01:CDS | 40.0% | |
| ATTTAGAGATGAACCGGAGT+TGG | - | Chr7:1953995-1954014 | MsG0780036060.01.T01:intron | 40.0% | |
| GAGAACAAGGGATTTGAATC+CGG | - | Chr7:1953822-1953841 | MsG0780036060.01.T01:intron | 40.0% | |
| GGTTCCTGTGAAGAAAAGAA+GGG | - | Chr7:1954121-1954140 | MsG0780036060.01.T01:CDS | 40.0% | |
| GTAGAATTTGGCGAGAAACA+CGG | + | Chr7:1953143-1953162 | None:intergenic | 40.0% | |
| GTATTGAAGGATTGTGGTGT+TGG | - | Chr7:1954146-1954165 | MsG0780036060.01.T01:CDS | 40.0% | |
| GTGTTTCTCGCCAAATTCTA+CGG | - | Chr7:1953142-1953161 | MsG0780036060.01.T01:CDS | 40.0% | |
| TCAGTGCATGTTTAGATCGA+CGG | - | Chr7:1953255-1953274 | MsG0780036060.01.T01:CDS | 40.0% | |
| TCCGAAGTTATTGGTGTGAT+CGG | + | Chr7:1953627-1953646 | None:intergenic | 40.0% | |
| TGGTTCCTGTGAAGAAAAGA+AGG | - | Chr7:1954120-1954139 | MsG0780036060.01.T01:CDS | 40.0% | |
| TGTCTCGAAAGAGGAAAACT+CGG | - | Chr7:1953698-1953717 | MsG0780036060.01.T01:CDS | 40.0% | |
| TTTCCTCTTTCGAGACATGA+CGG | + | Chr7:1953695-1953714 | None:intergenic | 40.0% | |
| ! | ACAACCCTTCTTTTCTTCAC+AGG | + | Chr7:1954128-1954147 | None:intergenic | 40.0% |
| ! | ACGGTTCAGTCGGTTTATTA+TGG | - | Chr7:1953547-1953566 | MsG0780036060.01.T01:CDS | 40.0% |
| ! | GCATAAGACCTTGTTTACAG+TGG | - | Chr7:1953086-1953105 | MsG0780036060.01.T01:CDS | 40.0% |
| ! | TCAGTCGGTTTATTATGGTC+CGG | - | Chr7:1953552-1953571 | MsG0780036060.01.T01:CDS | 40.0% |
| ! | TTGGTTTAGAGCTGACTCTT+GGG | - | Chr7:1954069-1954088 | MsG0780036060.01.T01:CDS | 40.0% |
| !! | GAGAAAGCCAATGGATTTTC+AGG | + | Chr7:1952849-1952868 | None:intergenic | 40.0% |
| !! | TGGCATTAGCTTGTGATTCA+GGG | + | Chr7:1953120-1953139 | None:intergenic | 40.0% |
| !!! | CGGTTCAGTTTTGACTTAAG+CGG | - | Chr7:1954228-1954247 | MsG0780036060.01.T01:exon | 40.0% |
| ACGTGATAAACGCGTGAAAC+CGG | + | Chr7:1954101-1954120 | None:intergenic | 45.0% | |
| AGAGAGAGAGAGAAAGCCAA+TGG | + | Chr7:1952858-1952877 | None:intergenic | 45.0% | |
| CAAGCCGAAGATACAAGAAG+AGG | - | Chr7:1953785-1953804 | MsG0780036060.01.T01:intron | 45.0% | |
| CTCAACAGCGAGTTAAGACT+CGG | - | Chr7:1953739-1953758 | MsG0780036060.01.T01:CDS | 45.0% | |
| CTCATGAACCTTGTTAACGC+CGG | - | Chr7:1953175-1953194 | MsG0780036060.01.T01:CDS | 45.0% | |
| CTCTTCTTGTATCTTCGGCT+TGG | + | Chr7:1953787-1953806 | None:intergenic | 45.0% | |
| GCCGATCACACCAATAACTT+CGG | - | Chr7:1953623-1953642 | MsG0780036060.01.T01:CDS | 45.0% | |
| GTTTATTATGGTCCGGAAGC+TGG | - | Chr7:1953559-1953578 | MsG0780036060.01.T01:CDS | 45.0% | |
| TCGTTGTTGTACGAAGCATG+TGG | - | Chr7:1953504-1953523 | MsG0780036060.01.T01:CDS | 45.0% | |
| TTCAATATCCGGCTTAGACC+CGG | - | Chr7:1954192-1954211 | MsG0780036060.01.T01:CDS | 45.0% | |
| TTGAGTTAACTCCGTAGACG+CGG | + | Chr7:1953724-1953743 | None:intergenic | 45.0% | |
| ! | GTTGGTTTAGAGCTGACTCT+TGG | - | Chr7:1954068-1954087 | MsG0780036060.01.T01:CDS | 45.0% |
| ! | TTCATTTTCCGGTCCAACTC+CGG | + | Chr7:1954011-1954030 | None:intergenic | 45.0% |
| !! | ATGTCATAGGCTTTGAGTGG+TGG | + | Chr7:1953672-1953691 | None:intergenic | 45.0% |
| !! | GTGGCATTAGCTTGTGATTC+AGG | + | Chr7:1953121-1953140 | None:intergenic | 45.0% |
| !! | TGTCATAGGCTTTGAGTGGT+GGG | + | Chr7:1953671-1953690 | None:intergenic | 45.0% |
| !!! | TTTTAAAACGGCTTCCACCG+CGG | + | Chr7:1953598-1953617 | None:intergenic | 45.0% |
| AACCGAAACCGAACCAAACC+GGG | + | Chr7:1954213-1954232 | None:intergenic | 50.0% | |
| ACCAGCACGACCGTAGAATT+TGG | + | Chr7:1953155-1953174 | None:intergenic | 50.0% | |
| ACTTCGGAAGCAGCTGCTAA+CGG | - | Chr7:1953639-1953658 | MsG0780036060.01.T01:CDS | 50.0% | |
| CATCCGTCATGTCTCGAAAG+AGG | - | Chr7:1953689-1953708 | MsG0780036060.01.T01:CDS | 50.0% | |
| CGAACCTGGACGAAGATGTT+CGG | + | Chr7:1953203-1953222 | None:intergenic | 50.0% | |
| GAACCGAAACCGAACCAAAC+CGG | + | Chr7:1954214-1954233 | None:intergenic | 50.0% | |
| GAACCTGGACGAAGATGTTC+GGG | + | Chr7:1953202-1953221 | None:intergenic | 50.0% | |
| GAATTTGGCGAGAAACACGG+TGG | + | Chr7:1953140-1953159 | None:intergenic | 50.0% | |
| GAGAGTGGAGAGGAAAAGGT+TGG | - | Chr7:1954050-1954069 | MsG0780036060.01.T01:intron | 50.0% | |
| GCAGCTGCTTCCGAAGTTAT+TGG | + | Chr7:1953636-1953655 | None:intergenic | 50.0% | |
| GCTCATGGAATGTGGAGTTG+GGG | - | Chr7:1954168-1954187 | MsG0780036060.01.T01:CDS | 50.0% | |
| GGAGTTGGGGCTTCAATATC+CGG | - | Chr7:1954181-1954200 | MsG0780036060.01.T01:CDS | 50.0% | |
| GGCTCATGGAATGTGGAGTT+GGG | - | Chr7:1954167-1954186 | MsG0780036060.01.T01:CDS | 50.0% | |
| GGTTTCACGCGTTTATCACG+TGG | - | Chr7:1954100-1954119 | MsG0780036060.01.T01:CDS | 50.0% | |
| GTTGGACCGGAAAATGAAGC+CGG | - | Chr7:1954013-1954032 | MsG0780036060.01.T01:intron | 50.0% | |
| TGGCTCATGGAATGTGGAGT+TGG | - | Chr7:1954166-1954185 | MsG0780036060.01.T01:CDS | 50.0% | |
| TTGACATAGCTGCCAGCTTC+CGG | + | Chr7:1953574-1953593 | None:intergenic | 50.0% | |
| ! | ACGACTCATGACTAGCTGAC+CGG | + | Chr7:1953896-1953915 | None:intergenic | 50.0% |
| ! | CTTTTCCTCTCCACTCTCAC+CGG | + | Chr7:1954048-1954067 | None:intergenic | 50.0% |
| ! | TTAAGACTCGGTCGCGTAAG+CGG | - | Chr7:1953751-1953770 | MsG0780036060.01.T01:CDS | 50.0% |
| !! | AGGATTGTGGTGTTGGCTCA+TGG | - | Chr7:1954153-1954172 | MsG0780036060.01.T01:CDS | 50.0% |
| !! | CGAGACATGACGGATGTCAT+AGG | + | Chr7:1953685-1953704 | None:intergenic | 50.0% |
| !! | CGGATGTCATAGGCTTTGAG+TGG | + | Chr7:1953675-1953694 | None:intergenic | 50.0% |
| !! | GATCGGCGCGCCTTTTAAAA+CGG | + | Chr7:1953610-1953629 | None:intergenic | 50.0% |
| !! | GCGGTGGAAGCCGTTTTAAA+AGG | - | Chr7:1953597-1953616 | MsG0780036060.01.T01:CDS | 50.0% |
| !! | GGTCAACCGGCTTCATTTTC+CGG | + | Chr7:1954022-1954041 | None:intergenic | 50.0% |
| !! | TGGTGTTGGCTCATGGAATG+TGG | - | Chr7:1954160-1954179 | MsG0780036060.01.T01:CDS | 50.0% |
| ATGAAGCCGGTTGACCGAAC+CGG | - | Chr7:1954026-1954045 | MsG0780036060.01.T01:intron | 55.0% | |
| CGGTGAGAGTGGAGAGGAAA+AGG | - | Chr7:1954046-1954065 | MsG0780036060.01.T01:intron | 55.0% | |
| CGGTTCATCACCTCCTCAAC+CGG | + | Chr7:1953876-1953895 | None:intergenic | 55.0% | |
| CGGTTGAGGAGGTGATGAAC+CGG | - | Chr7:1953874-1953893 | MsG0780036060.01.T01:intron | 55.0% | |
| CTTCGGAAGCAGCTGCTAAC+GGG | - | Chr7:1953640-1953659 | MsG0780036060.01.T01:CDS | 55.0% | |
| GAACCAAACCGGGTCTAAGC+CGG | + | Chr7:1954203-1954222 | None:intergenic | 55.0% | |
| GAGATGAACCGGAGTTGGAC+CGG | - | Chr7:1954000-1954019 | MsG0780036060.01.T01:intron | 55.0% | |
| GCCAAATTCTACGGTCGTGC+TGG | - | Chr7:1953151-1953170 | MsG0780036060.01.T01:CDS | 55.0% | |
| TATCCGGCTTAGACCCGGTT+TGG | - | Chr7:1954197-1954216 | MsG0780036060.01.T01:CDS | 55.0% | |
| TGGACGAAGATGTTCGGGAC+CGG | + | Chr7:1953197-1953216 | None:intergenic | 55.0% | |
| TTCAACTGAGCCGACCGGTT+CGG | - | Chr7:1953845-1953864 | MsG0780036060.01.T01:intron | 55.0% | |
| TTCGGTTGAACCGGTTGAGG+AGG | - | Chr7:1953863-1953882 | MsG0780036060.01.T01:intron | 55.0% | |
| ! | CTCAACCGGTTCAACCGAAC+CGG | + | Chr7:1953862-1953881 | None:intergenic | 55.0% |
| ! | GATTAGCCACCAGTCTGAGG+CGG | - | Chr7:1953917-1953936 | MsG0780036060.01.T01:intron | 55.0% |
| ! | GCTGACTCTTGGGCTTGAAC+CGG | - | Chr7:1954079-1954098 | MsG0780036060.01.T01:CDS | 55.0% |
| ! | GTCGATTAGCCACCAGTCTG+AGG | - | Chr7:1953914-1953933 | MsG0780036060.01.T01:intron | 55.0% |
| !! | GACCCGGTTTGGTTCGGTTT+CGG | - | Chr7:1954208-1954227 | MsG0780036060.01.T01:CDS | 55.0% |
| !! | GGCTTAGACCCGGTTTGGTT+CGG | - | Chr7:1954202-1954221 | MsG0780036060.01.T01:CDS | 55.0% |
| CTGGCAGCTATGTCAAGCCG+CGG | - | Chr7:1953578-1953597 | MsG0780036060.01.T01:CDS | 60.0% | |
| GAAGCAGCTGCTAACGGGAG+GGG | - | Chr7:1953645-1953664 | MsG0780036060.01.T01:CDS | 60.0% | |
| GGAAAACTCGGCCGCGTCTA+CGG | - | Chr7:1953710-1953729 | MsG0780036060.01.T01:CDS | 60.0% | |
| GGAAGCAGCTGCTAACGGGA+GGG | - | Chr7:1953644-1953663 | MsG0780036060.01.T01:CDS | 60.0% | |
| GGTCCCGAACATCTTCGTCC+AGG | - | Chr7:1953196-1953215 | MsG0780036060.01.T01:CDS | 60.0% | |
| GTTCGGGACCGGCGTTAACA+AGG | + | Chr7:1953186-1953205 | None:intergenic | 60.0% | |
| GTTGACCGAACCGGTGAGAG+TGG | - | Chr7:1954035-1954054 | MsG0780036060.01.T01:intron | 60.0% | |
| TCCGGTTCAACTGAGCCGAC+CGG | - | Chr7:1953840-1953859 | MsG0780036060.01.T01:intron | 60.0% | |
| ! | ACCGGTCGGCTCAGTTGAAC+CGG | + | Chr7:1953844-1953863 | None:intergenic | 60.0% |
| ! | ACCGGTTCAACCGAACCGGT+CGG | + | Chr7:1953858-1953877 | None:intergenic | 60.0% |
| ! | CCTCTCCACTCTCACCGGTT+CGG | + | Chr7:1954043-1954062 | None:intergenic | 60.0% |
| !! | CGGTTCGGTTGAACCGGTTG+AGG | - | Chr7:1953860-1953879 | MsG0780036060.01.T01:intron | 60.0% |
| !! | CTCTCACCGGTTCGGTCAAC+CGG | + | Chr7:1954035-1954054 | None:intergenic | 60.0% |
| CCGAACCGGTGAGAGTGGAG+AGG | - | Chr7:1954040-1954059 | MsG0780036060.01.T01:intron | 65.0% | |
| CGGAAGCAGCTGCTAACGGG+AGG | - | Chr7:1953643-1953662 | MsG0780036060.01.T01:CDS | 65.0% | |
| GCAGCTATGTCAAGCCGCGG+TGG | - | Chr7:1953581-1953600 | MsG0780036060.01.T01:CDS | 65.0% | |
| ! | CCACCAGTCTGAGGCGGTTG+CGG | - | Chr7:1953923-1953942 | MsG0780036060.01.T01:intron | 65.0% |
| !! | GCCGACCGGTTCGGTTGAAC+CGG | - | Chr7:1953854-1953873 | MsG0780036060.01.T01:intron | 65.0% |
| CCACCGCAACCGCCTCAGAC+TGG | + | Chr7:1953929-1953948 | None:intergenic | 70.0% | |
| CCGCAACCGCCTCAGACTGG+TGG | + | Chr7:1953926-1953945 | None:intergenic | 70.0% | |
| ! | CCAGTCTGAGGCGGTTGCGG+TGG | - | Chr7:1953926-1953945 | MsG0780036060.01.T01:intron | 70.0% |
| ! | GTCTGAGGCGGTTGCGGTGG+TGG | - | Chr7:1953929-1953948 | MsG0780036060.01.T01:intron | 70.0% |
| !! | GAGGCGGTTGCGGTGGTGGA+TGG | - | Chr7:1953933-1953952 | MsG0780036060.01.T01:intron | 70.0% |
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| Chr7 | gene | 1952822 | 1954450 | 1952822 | ID=MsG0780036060.01;Name=MsG0780036060.01 |
| Chr7 | mRNA | 1952822 | 1954450 | 1952822 | ID=MsG0780036060.01.T01;Parent=MsG0780036060.01;Name=MsG0780036060.01.T01;_AED=0.33;_eAED=0.33;_QI=206|1|1|1|1|1|2|242|301 |
| Chr7 | exon | 1952822 | 1953779 | 1952822 | ID=MsG0780036060.01.T01:exon:38386;Parent=MsG0780036060.01.T01 |
| Chr7 | exon | 1954055 | 1954450 | 1954055 | ID=MsG0780036060.01.T01:exon:38385;Parent=MsG0780036060.01.T01 |
| Chr7 | five_prime_UTR | 1954245 | 1954450 | 1954245 | ID=MsG0780036060.01.T01:five_prime_utr;Parent=MsG0780036060.01.T01 |
| Chr7 | CDS | 1954055 | 1954244 | 1954055 | ID=MsG0780036060.01.T01:cds;Parent=MsG0780036060.01.T01 |
| Chr7 | CDS | 1953064 | 1953779 | 1953064 | ID=MsG0780036060.01.T01:cds;Parent=MsG0780036060.01.T01 |
| Chr7 | three_prime_UTR | 1952822 | 1953063 | 1952822 | ID=MsG0780036060.01.T01:three_prime_utr;Parent=MsG0780036060.01.T01 |
| Gene Sequence |
| Protein sequence |
