AlfalfaGEDB | MsG0280010751.01

M. sativa cultivar ZhongmuNo.1 / MsG0280010751.01

KEGG: K23047
GO: GO:0046872
PFAM: PF00642

Nr:

Query id	Subject id	identity %	alignment length	mis matches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MsG0280010751.01.T02	PNX73613.1	85.714	161	6	2	1	148	78	234	9.12E-90	277
MsG0280010751.01.T03	PNX73613.1	98.319	119	2	0	1	119	78	196	4.71E-82	256
MsG0280010751.01.T01	XP_013465134.1	88.571	175	1	2	7	164	24	196	6.19E-106	319

Swissprot:

Query id	Subject id	identity %	alignment length	mis matches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MsG0280010751.01.T01	Q9ZWA1	88.596	114	12	1	36	148	75	188	8.81E-73	226
MsG0280010751.01.T02	Q9ZWA1	72	150	30	2	1	149	85	223	1.18E-70	221
MsG0280010751.01.T03	Q9ZWA1	88.462	104	11	1	1	103	85	188	6.77E-65	204

Trembl:

Query id	Subject id	identity %	alignment length	mis matches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MsG0280010751.01.T01	A0A072VAW2	88.571	175	1	2	7	164	24	196	2.95e-106	319
MsG0280010751.01.T02	A0A2K3PI35	85.714	161	6	2	1	148	78	234	4.36e-90	277
MsG0280010751.01.T03	A0A2K3PI35	98.319	119	2	0	1	119	78	196	2.25e-82	256

TFs/TRs:

Gene ID	Type	Classification
MsG0280010751.01.T02	TF	C3H
MsG0280010751.01.T01	TF	C3H
MsG0280010751.01.T03	TF	C3H

Protein Kinases:

Gene ID	Type	Classification

Network:

Co-expression Network

Gene1	Gene2	correlation coefficient	p_value	FDR
MsG0280010751.01	MsG0380017483.01	0.805076	1.589130e-49	8.329632e-47
MsG0280010751.01	MsG0480021968.01	0.812815	3.505742e-51	2.251061e-48
MsG0280010751.01	MsG0880047704.01	0.815222	1.032504e-51	7.076109e-49

PPI

Gene1	Gene2	Type

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MsG0280010751.01.T02	MTR_2g090605	87.730	163	3	3	1	148	80	240	2.37e-88	264
MsG0280010751.01.T02	MTR_4g078660	74.809	131	32	1	1	131	63	192	1.28e-66	206
MsG0280010751.01.T02	MTR_6g090380	77.228	101	23	0	1	101	106	206	1.88e-58	186
MsG0280010751.01.T02	MTR_7g092070	68.807	109	34	0	1	109	267	375	1.61e-56	188
MsG0280010751.01.T02	MTR_8g102100	74.000	100	26	0	1	100	84	183	1.26e-55	179
MsG0280010751.01.T02	MTR_3g029590	66.972	109	36	0	1	109	267	375	1.74e-54	183
MsG0280010751.01.T02	MTR_3g103960	65.217	115	35	2	1	114	56	166	8.67e-52	166
MsG0280010751.01.T02	MTR_5g082830	64.545	110	37	1	1	110	220	327	3.27e-51	173
MsG0280010751.01.T02	MTR_5g071070	60.550	109	43	0	1	109	151	259	1.90e-50	171
MsG0280010751.01.T02	MTR_1g050438	63.889	108	39	0	1	108	231	338	1.91e-47	162
MsG0280010751.01.T02	MTR_0728s0020	63.889	108	39	0	1	108	247	354	3.13e-47	162
MsG0280010751.01.T02	MTR_5g071070	60.550	109	43	0	1	109	233	341	1.19e-46	161
MsG0280010751.01.T02	MTR_3g070880	54.167	120	55	0	1	120	166	285	1.19e-45	156
MsG0280010751.01.T02	MTR_1g075890	60.550	109	43	0	1	109	232	340	5.81e-45	156
MsG0280010751.01.T02	MTR_3g435680	61.468	109	42	0	1	109	154	262	7.81e-45	152
MsG0280010751.01.T02	MTR_3g435590	60.000	100	40	0	1	100	241	340	6.02e-40	140
MsG0280010751.01.T02	MTR_3g464270	50.909	110	53	1	1	109	12	121	1.09e-34	124
MsG0280010751.01.T02	MTR_3g464260	62.162	74	28	0	29	102	212	285	2.43e-29	112
MsG0280010751.01.T02	MTR_3g464290	56.410	78	33	1	1	77	103	180	8.21e-27	105
MsG0280010751.01.T02	MTR_3g464280	39.216	102	28	1	1	102	191	258	4.55e-17	77.8
MsG0280010751.01.T03	MTR_2g090605	98.319	119	0	1	1	119	80	196	4.01e-84	251
MsG0280010751.01.T03	MTR_4g078660	86.139	101	14	0	1	101	63	163	1.62e-64	198
MsG0280010751.01.T03	MTR_6g090380	77.228	101	23	0	1	101	106	206	4.39e-59	186
MsG0280010751.01.T03	MTR_7g092070	68.807	109	34	0	1	109	267	375	8.65e-57	187
MsG0280010751.01.T03	MTR_8g102100	74.000	100	26	0	1	100	84	183	4.24e-56	179
MsG0280010751.01.T03	MTR_3g103960	65.217	115	35	2	1	114	56	166	8.84e-53	167
MsG0280010751.01.T03	MTR_3g029590	66.972	109	36	0	1	109	267	375	9.86e-52	174
MsG0280010751.01.T03	MTR_5g082830	64.545	110	37	1	1	110	220	327	5.92e-51	171
MsG0280010751.01.T03	MTR_1g050438	63.889	108	39	0	1	108	231	338	8.07e-51	169
MsG0280010751.01.T03	MTR_0728s0020	63.889	108	39	0	1	108	247	354	1.21e-50	169
MsG0280010751.01.T03	MTR_5g071070	60.550	109	43	0	1	109	151	259	1.42e-47	161
MsG0280010751.01.T03	MTR_5g071070	60.550	109	43	0	1	109	233	341	3.67e-47	160
MsG0280010751.01.T03	MTR_1g075890	60.550	109	43	0	1	109	232	340	1.28e-45	156
MsG0280010751.01.T03	MTR_3g435680	61.468	109	42	0	1	109	154	262	2.30e-45	151
MsG0280010751.01.T03	MTR_3g070880	56.881	109	47	0	1	109	166	274	1.29e-42	146
MsG0280010751.01.T03	MTR_3g435590	60.000	100	40	0	1	100	241	340	1.29e-40	140
MsG0280010751.01.T03	MTR_3g464270	50.909	110	53	1	1	109	12	121	7.18e-36	125
MsG0280010751.01.T03	MTR_3g464260	61.333	75	29	0	28	102	211	285	1.02e-29	111
MsG0280010751.01.T03	MTR_3g464290	55.000	80	35	1	1	79	103	182	1.23e-27	105
MsG0280010751.01.T03	MTR_3g464280	39.216	102	28	1	1	102	191	258	1.02e-17	77.8
MsG0280010751.01.T01	MTR_2g090605	88.571	175	1	2	7	164	24	196	7.49e-110	319
MsG0280010751.01.T01	MTR_4g078660	82.963	135	21	1	14	146	29	163	3.34e-83	248
MsG0280010751.01.T01	MTR_6g090380	74.380	121	29	1	26	146	88	206	1.98e-65	204
MsG0280010751.01.T01	MTR_8g102100	74.312	109	28	0	37	145	75	183	7.47e-62	196
MsG0280010751.01.T01	MTR_7g092070	57.823	147	60	1	10	154	229	375	5.01e-58	192
MsG0280010751.01.T01	MTR_3g103960	64.754	122	38	2	39	159	49	166	1.71e-56	179
MsG0280010751.01.T01	MTR_3g029590	66.379	116	39	0	39	154	260	375	1.15e-55	186
MsG0280010751.01.T01	MTR_1g050438	64.348	115	41	0	39	153	224	338	2.99e-52	175
MsG0280010751.01.T01	MTR_5g082830	64.957	117	39	1	39	155	213	327	2.99e-52	176
MsG0280010751.01.T01	MTR_0728s0020	64.348	115	41	0	39	153	240	354	5.36e-52	175
MsG0280010751.01.T01	MTR_5g071070	58.140	129	51	1	29	154	131	259	5.42e-52	175
MsG0280010751.01.T01	MTR_5g071070	52.632	152	67	2	8	154	190	341	1.41e-51	175
MsG0280010751.01.T01	MTR_1g075890	61.207	116	45	0	39	154	225	340	7.75e-50	169
MsG0280010751.01.T01	MTR_3g435680	61.207	116	45	0	39	154	147	262	4.24e-49	163
MsG0280010751.01.T01	MTR_3g070880	54.167	120	55	0	35	154	155	274	1.50e-45	156
MsG0280010751.01.T01	MTR_3g435590	59.813	107	43	0	39	145	234	340	1.36e-44	153
MsG0280010751.01.T01	MTR_3g464270	50.877	114	55	1	42	154	8	121	2.98e-38	134
MsG0280010751.01.T01	MTR_3g464290	53.933	89	40	1	36	123	93	181	5.13e-31	116
MsG0280010751.01.T01	MTR_3g464260	61.333	75	29	0	73	147	211	285	8.38e-30	114
MsG0280010751.01.T01	MTR_3g464280	40.179	112	33	1	36	147	181	258	6.53e-22	91.7

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MsG0280010751.01.T02	AT1G03790	72.000	150	30	2	1	149	85	223	1.20e-71	221
MsG0280010751.01.T02	AT5G44260	66.667	156	29	3	1	144	63	207	2.53e-65	204
MsG0280010751.01.T02	AT2G19810	78.302	106	23	0	1	106	81	186	7.54e-61	192
MsG0280010751.01.T02	AT2G25900	76.699	103	24	0	1	103	93	195	1.35e-60	190
MsG0280010751.01.T02	AT2G25900	76.699	103	24	0	1	103	93	195	1.35e-60	190
MsG0280010751.01.T02	AT4G29190	78.431	102	22	0	1	102	82	183	3.27e-60	190
MsG0280010751.01.T02	AT2G41900	67.890	109	35	0	1	109	264	372	1.78e-55	185
MsG0280010751.01.T02	AT5G12850	64.815	108	37	1	1	107	260	367	4.21e-51	173
MsG0280010751.01.T02	AT5G58620	67.593	108	34	1	1	107	212	319	9.96e-51	171
MsG0280010751.01.T02	AT5G07500	69.000	100	31	0	1	100	55	154	1.97e-48	157
MsG0280010751.01.T02	AT2G40140	49.650	143	69	1	1	143	217	356	1.78e-44	154
MsG0280010751.01.T02	AT2G40140	49.650	143	69	1	1	143	217	356	1.78e-44	154
MsG0280010751.01.T02	AT3G55980	60.000	110	42	1	1	110	214	321	4.93e-44	153
MsG0280010751.01.T02	AT3G55980	60.000	110	42	1	1	110	281	388	1.05e-43	152
MsG0280010751.01.T03	AT1G03790	88.462	104	11	1	1	103	85	188	6.90e-66	204
MsG0280010751.01.T03	AT5G44260	85.577	104	14	1	1	103	63	166	2.32e-63	197
MsG0280010751.01.T03	AT2G19810	78.302	106	23	0	1	106	81	186	1.49e-61	192
MsG0280010751.01.T03	AT2G25900	76.699	103	24	0	1	103	93	195	4.49e-61	189
MsG0280010751.01.T03	AT2G25900	76.699	103	24	0	1	103	93	195	4.49e-61	189
MsG0280010751.01.T03	AT4G29190	78.431	102	22	0	1	102	82	183	5.50e-61	191
MsG0280010751.01.T03	AT2G41900	67.890	109	35	0	1	109	264	372	1.70e-55	184
MsG0280010751.01.T03	AT5G58620	67.593	108	34	1	1	107	212	319	2.85e-51	171
MsG0280010751.01.T03	AT5G12850	64.815	108	37	1	1	107	260	367	4.58e-51	171
MsG0280010751.01.T03	AT5G07500	69.000	100	31	0	1	100	55	154	3.33e-49	157
MsG0280010751.01.T03	AT2G40140	60.909	110	41	1	1	110	217	324	9.83e-45	153
MsG0280010751.01.T03	AT2G40140	60.909	110	41	1	1	110	217	324	9.83e-45	153
MsG0280010751.01.T03	AT3G55980	60.000	110	42	1	1	110	214	321	2.16e-44	152
MsG0280010751.01.T03	AT3G55980	60.000	110	42	1	1	110	281	388	3.04e-44	152
MsG0280010751.01.T01	AT1G03790	88.596	114	12	1	36	148	75	188	8.98e-74	226
MsG0280010751.01.T01	AT5G44260	70.861	151	29	3	13	148	16	166	3.22e-72	222
MsG0280010751.01.T01	AT2G19810	77.391	115	26	0	37	151	72	186	6.39e-67	208
MsG0280010751.01.T01	AT4G29190	74.167	120	28	1	31	147	64	183	1.31e-66	207
MsG0280010751.01.T01	AT2G25900	75.893	112	27	0	37	148	84	195	9.24e-66	204
MsG0280010751.01.T01	AT2G25900	75.893	112	27	0	37	148	84	195	9.24e-66	204
MsG0280010751.01.T01	AT2G41900	67.241	116	38	0	39	154	257	372	9.98e-57	189
MsG0280010751.01.T01	AT5G58620	66.379	116	38	1	39	153	205	320	3.57e-55	183
MsG0280010751.01.T01	AT5G07500	68.468	111	35	0	35	145	44	154	2.64e-54	172
MsG0280010751.01.T01	AT5G12850	65.217	115	39	1	39	152	253	367	2.23e-53	180
MsG0280010751.01.T01	AT3G55980	58.678	121	48	1	35	155	203	321	9.39e-49	166
MsG0280010751.01.T01	AT3G55980	58.678	121	48	1	35	155	270	388	1.96e-48	166
MsG0280010751.01.T01	AT2G40140	60.684	117	44	1	39	155	210	324	3.45e-48	164
MsG0280010751.01.T01	AT2G40140	60.684	117	44	1	39	155	210	324	3.45e-48	164

Find 51 sgRNAs with CRISPR-Local

Find 68 sgRNAs with CRISPR-GE

CRISPR-Local

CRISPR-Local
sgRNA_sequence	on_target_score	Position	Region
CGGCGGGTGAGGAGCTTTCT+TGG	0.183749	2:+74369839	None:intergenic
GAGGGATGTCGATGTCTCTA+AGG	0.318194	2:+74369861	None:intergenic
CGGTGGTGTTGCGGCGGGTG+AGG	0.397490	2:+74369828	None:intergenic
TTTGGGAGAGAGAGGTTGGT+AGG	0.411129	2:+74369888	None:intergenic
CTCGTAGCCGGAGCCATGAC+TGG	0.421786	2:-74369675	MsG0280010751.01.T01:CDS
CGCCGCGGTGGCTGTAACCG+TGG	0.429136	2:-74369562	MsG0280010751.01.T01:CDS
GAAGATGATGATGATGTAAC+AGG	0.430284	2:+74369397	None:intergenic
CAGCCACCGCGGCGATACTC+AGG	0.436266	2:+74369571	None:intergenic
GAAGATGTCGACGGCGGCGG+TGG	0.446476	2:+74369811	None:intergenic
GGACTTTGAATTCATACATT+CGG	0.463131	2:+74369707	None:intergenic
CGGCGGCGGTGGTGTTGCGG+CGG	0.463780	2:+74369822	None:intergenic
GGCGGCGGTGGTGTTGCGGC+GGG	0.472525	2:+74369823	None:intergenic
GGAGAAGATGTCGACGGCGG+CGG	0.481474	2:+74369808	None:intergenic
CGGGTGAGGAGCTTTCTTGG+AGG	0.487835	2:+74369842	None:intergenic
CGGAAATGATCTGATGAGTA+AGG	0.490767	2:+74369727	None:intergenic
GGGTGAGGAGCTTTCTTGGA+GGG	0.496924	2:+74369843	None:intergenic
AGAACTGAAGCATGCAAAGA+TGG	0.503562	2:-74369481	MsG0280010751.01.T01:CDS
TCCGCCGGTGCACTCGTAGC+CGG	0.504444	2:-74369687	MsG0280010751.01.T01:CDS
CGACGGCGGCGGTGGTGTTG+CGG	0.519055	2:+74369819	None:intergenic
CGTGGCTTCATCGGAGACAT+AGG	0.522416	2:+74369193	None:intergenic
CCACGGCGGTTTCATTACTC+AGG	0.527158	2:-74369604	MsG0280010751.01.T01:CDS
CACTGTGGCGGTAATGGTAA+TGG	0.535187	2:-74369288	MsG0280010751.01.T02:CDS
ATCTGATGAGTAAGGATCAT+CGG	0.544740	2:+74369735	None:intergenic
GCTTCAGTTCTGTATCTAGC+AGG	0.547143	2:+74369493	None:intergenic
GGCGGTAATGGTAATGGTAA+TGG	0.562964	2:-74369282	MsG0280010751.01.T02:CDS
AATGAAACCGCCGTGGATCA+CGG	0.566037	2:+74369611	None:intergenic
AAGCTCGCCGCCGTGATCCA+CGG	0.567851	2:-74369621	MsG0280010751.01.T01:CDS
AGTCAGTCCAGTCATGGCTC+CGG	0.569804	2:+74369668	None:intergenic
GGAGACATAGGTGGTGATGC+CGG	0.581592	2:+74369205	None:intergenic
GATACTTGTGAATTTGCTCA+TGG	0.582670	2:-74369538	MsG0280010751.01.T01:CDS
ATGGACAGTCAGTCCAGTCA+TGG	0.586583	2:+74369662	None:intergenic
AGCCACCGCGGCGATACTCA+GGG	0.587575	2:+74369572	None:intergenic
GACTGTCCATTCGCTCACCC+CGG	0.597229	2:-74369649	MsG0280010751.01.T01:CDS
AGGGATGTCGATGTCTCTAA+GGG	0.602570	2:+74369862	None:intergenic
TCCGGCTACGAGTGCACCGG+CGG	0.607263	2:+74369686	None:intergenic
GGCTCCGGCTACGAGTGCAC+CGG	0.627207	2:+74369683	None:intergenic
ATGATGATGATGTAACAGGA+AGG	0.628815	2:+74369401	None:intergenic
TGCCCTGAGTATCGCCGCGG+TGG	0.630381	2:-74369574	MsG0280010751.01.T01:CDS
CACGGCGGTTTCATTACTCA+GGG	0.632004	2:-74369603	MsG0280010751.01.T01:CDS
CAAATTCACAAGTATCGCCA+CGG	0.650826	2:+74369545	None:intergenic
GACATAGGTGGTGATGCCGG+AGG	0.666150	2:+74369208	None:intergenic
GTCGTCGGAGAAGATGTCGA+CGG	0.666964	2:+74369802	None:intergenic
GGAAGGATTCTTAATTGACG+AGG	0.669701	2:+74369418	None:intergenic
TGTATGAATTCAAAGTCCGC+CGG	0.674896	2:-74369702	MsG0280010751.01.T01:CDS
GTCGGAGAAGATGTCGACGG+CGG	0.678761	2:+74369805	None:intergenic
GGCTTCATCGGAGACATAGG+TGG	0.681714	2:+74369196	None:intergenic
CCTGAGTAATGAAACCGCCG+TGG	0.683137	2:+74369604	None:intergenic
CTCGCCGCCGTGATCCACGG+CGG	0.698305	2:-74369618	MsG0280010751.01.T01:CDS
CGCCACGGTTACAGCCACCG+CGG	0.740750	2:+74369560	None:intergenic
GAAACCGCCGTGGATCACGG+CGG	0.763141	2:+74369614	None:intergenic
GTTTGCCCTGAGTATCGCCG+CGG	0.768550	2:-74369577	MsG0280010751.01.T01:CDS

CRISPR-GE

badsite warning	sgRNA_sequence	Strand	Position	Region	GC_content
	GGACTTTGAATTCATACATT+CGG	+	Chr2:74369383-74369402	None:intergenic	30.0%
!!	ATACCAAAAAGTGTTGAAGT+TGG	+	Chr2:74369844-74369863	None:intergenic	30.0%
	ATCTGATGAGTAAGGATCAT+CGG	+	Chr2:74369355-74369374	None:intergenic	35.0%
!	ATGATGATGATGTAACAGGA+AGG	+	Chr2:74369689-74369708	None:intergenic	35.0%
!	GAAGATGATGATGATGTAAC+AGG	+	Chr2:74369693-74369712	None:intergenic	35.0%
!	GATACTTGTGAATTTGCTCA+TGG	-	Chr2:74369549-74369568	MsG0280010751.01.T01:CDS	35.0%
!	TCTCCAACTTCAACACTTTT+TGG	-	Chr2:74369838-74369857	MsG0280010751.01.T01:CDS	35.0%
!!	GATGTCTCTAAGGGATTTTT+GGG	+	Chr2:74369219-74369238	None:intergenic	35.0%
!!!	TGTTGTTTGTTTTGTCACTG+TGG	-	Chr2:74369784-74369803	MsG0280010751.01.T01:intron	35.0%
	AGAACTGAAGCATGCAAAGA+TGG	-	Chr2:74369606-74369625	MsG0280010751.01.T01:CDS	40.0%
	CGGAAATGATCTGATGAGTA+AGG	+	Chr2:74369363-74369382	None:intergenic	40.0%
	GGAAGGATTCTTAATTGACG+AGG	+	Chr2:74369672-74369691	None:intergenic	40.0%
	TGTATGAATTCAAAGTCCGC+CGG	-	Chr2:74369385-74369404	MsG0280010751.01.T01:CDS	40.0%
!	ATTGTGTGAGGAACTTTTGG+AGG	+	Chr2:74369316-74369335	None:intergenic	40.0%
!	CAAATTCACAAGTATCGCCA+CGG	+	Chr2:74369545-74369564	None:intergenic	40.0%
!	GTCATTGTGTGAGGAACTTT+TGG	+	Chr2:74369319-74369338	None:intergenic	40.0%
!!	CGATGTCTCTAAGGGATTTT+TGG	+	Chr2:74369220-74369239	None:intergenic	40.0%
!!	CTCATGGTGTTTTTGAATGC+TGG	-	Chr2:74369565-74369584	MsG0280010751.01.T01:CDS	40.0%
!!	TTCTCATTTTTCTTCGCCTC+CGG	-	Chr2:74369863-74369882	MsG0280010751.01.T01:CDS	40.0%
!!!	TGTTTGTTTTGTCACTGTGG+CGG	-	Chr2:74369787-74369806	MsG0280010751.01.T01:intron	40.0%
	AGGGATGTCGATGTCTCTAA+GGG	+	Chr2:74369228-74369247	None:intergenic	45.0%
	GCTTCAGTTCTGTATCTAGC+AGG	+	Chr2:74369597-74369616	None:intergenic	45.0%
	TGAGTCAGAGTCATTGTGTG+AGG	+	Chr2:74369328-74369347	None:intergenic	45.0%
!	TTTTGTCACTGTGGCGGTAA+TGG	-	Chr2:74369793-74369812	MsG0280010751.01.T01:intron	45.0%
!!	AAGGGATTTTTGGGAGAGAG+AGG	+	Chr2:74369210-74369229	None:intergenic	45.0%
!!	GATTTTTGGGAGAGAGAGGT+TGG	+	Chr2:74369206-74369225	None:intergenic	45.0%
!!	GGCGGTAATGGTAATGGTAA+TGG	-	Chr2:74369805-74369824	MsG0280010751.01.T01:CDS	45.0%
!!	TGTGAGGAACTTTTGGAGGA+TGG	+	Chr2:74369312-74369331	None:intergenic	45.0%
	AATGAAACCGCCGTGGATCA+CGG	+	Chr2:74369479-74369498	None:intergenic	50.0%
	ATGGACAGTCAGTCCAGTCA+TGG	+	Chr2:74369428-74369447	None:intergenic	50.0%
	CACGGCGGTTTCATTACTCA+GGG	-	Chr2:74369484-74369503	MsG0280010751.01.T01:CDS	50.0%
	GAGGGATGTCGATGTCTCTA+AGG	+	Chr2:74369229-74369248	None:intergenic	50.0%
!	CACTGTGGCGGTAATGGTAA+TGG	-	Chr2:74369799-74369818	MsG0280010751.01.T01:intron	50.0%
!!!	CTTTTGGAGGATGGTGTCGT+CGG	+	Chr2:74369303-74369322	None:intergenic	50.0%
	AGTCAGTCCAGTCATGGCTC+CGG	+	Chr2:74369422-74369441	None:intergenic	55.0%
	CCACGGCGGTTTCATTACTC+AGG	-	Chr2:74369483-74369502	MsG0280010751.01.T01:CDS	55.0%
	CCTGAGTAATGAAACCGCCG+TGG	+	Chr2:74369486-74369505	None:intergenic	55.0%
	GGGTGAGGAGCTTTCTTGGA+GGG	+	Chr2:74369247-74369266	None:intergenic	55.0%
	GTCGTCGGAGAAGATGTCGA+CGG	+	Chr2:74369288-74369307	None:intergenic	55.0%
!	GGAGACATAGGTGGTGATGC+CGG	+	Chr2:74369885-74369904	None:intergenic	55.0%
!	TTTTCACCGGGGTGAGCGAA+TGG	+	Chr2:74369447-74369466	None:intergenic	55.0%
	CGGGTGAGGAGCTTTCTTGG+AGG	+	Chr2:74369248-74369267	None:intergenic	60.0%
	GACTGTCCATTCGCTCACCC+CGG	-	Chr2:74369438-74369457	MsG0280010751.01.T01:CDS	60.0%
	GTCGGAGAAGATGTCGACGG+CGG	+	Chr2:74369285-74369304	None:intergenic	60.0%
!	GACATAGGTGGTGATGCCGG+AGG	+	Chr2:74369882-74369901	None:intergenic	60.0%
!	GTTTGCCCTGAGTATCGCCG+CGG	-	Chr2:74369510-74369529	MsG0280010751.01.T01:CDS	60.0%
!!!	ACGGCGGCGAGCTTTTTCAC+CGG	+	Chr2:74369460-74369479	None:intergenic	60.0%
	AAGCTCGCCGCCGTGATCCA+CGG	-	Chr2:74369466-74369485	MsG0280010751.01.T01:CDS	65.0%
	AGCCACCGCGGCGATACTCA+GGG	+	Chr2:74369518-74369537	None:intergenic	65.0%
	CGGCGGGTGAGGAGCTTTCT+TGG	+	Chr2:74369251-74369270	None:intergenic	65.0%
	CTCGTAGCCGGAGCCATGAC+TGG	-	Chr2:74369412-74369431	MsG0280010751.01.T01:CDS	65.0%
	GAAACCGCCGTGGATCACGG+CGG	+	Chr2:74369476-74369495	None:intergenic	65.0%
	GGAGAAGATGTCGACGGCGG+CGG	+	Chr2:74369282-74369301	None:intergenic	65.0%
!!!	CGGCGGCGAGCTTTTTCACC+GGG	+	Chr2:74369459-74369478	None:intergenic	65.0%
!!!	GGCGGCGAGCTTTTTCACCG+GGG	+	Chr2:74369458-74369477	None:intergenic	65.0%
	CAGCCACCGCGGCGATACTC+AGG	+	Chr2:74369519-74369538	None:intergenic	70.0%
	CGCCACGGTTACAGCCACCG+CGG	+	Chr2:74369530-74369549	None:intergenic	70.0%
	GAAGATGTCGACGGCGGCGG+TGG	+	Chr2:74369279-74369298	None:intergenic	70.0%
	GGCTCCGGCTACGAGTGCAC+CGG	+	Chr2:74369407-74369426	None:intergenic	70.0%
	TCCGCCGGTGCACTCGTAGC+CGG	-	Chr2:74369400-74369419	MsG0280010751.01.T01:CDS	70.0%
	TCCGGCTACGAGTGCACCGG+CGG	+	Chr2:74369404-74369423	None:intergenic	70.0%
!	TGCCCTGAGTATCGCCGCGG+TGG	-	Chr2:74369513-74369532	MsG0280010751.01.T01:CDS	70.0%
	CGCCGCGGTGGCTGTAACCG+TGG	-	Chr2:74369525-74369544	MsG0280010751.01.T01:CDS	75.0%
	CTCGCCGCCGTGATCCACGG+CGG	-	Chr2:74369469-74369488	MsG0280010751.01.T01:CDS	75.0%
!	CGACGGCGGCGGTGGTGTTG+CGG	+	Chr2:74369271-74369290	None:intergenic	75.0%
!!	CGGTGGTGTTGCGGCGGGTG+AGG	+	Chr2:74369262-74369281	None:intergenic	75.0%
!!!	CGGCGGCGGTGGTGTTGCGG+CGG	+	Chr2:74369268-74369287	None:intergenic	80.0%
!!!	GGCGGCGGTGGTGTTGCGGC+GGG	+	Chr2:74369267-74369286	None:intergenic	80.0%

Chromosome	Type	Strat	End	Strand	Name
Chr2	gene	74369200	74369909	74369200	ID=MsG0280010751.01;Name=MsG0280010751.01
Chr2	mRNA	74369354	74369909	74369354	ID=MsG0280010751.01.T01;Parent=MsG0280010751.01;Name=MsG0280010751.01.T01;_AED=0.35;_eAED=0.35;_QI=0\|0\|0\|1\|0\|0\|2\|0\|168
Chr2	exon	74369354	74369754	74369354	ID=MsG0280010751.01.T01:exon:7557;Parent=MsG0280010751.01.T01
Chr2	exon	74369804	74369909	74369804	ID=MsG0280010751.01.T01:exon:7556;Parent=MsG0280010751.01.T01
Chr2	CDS	74369804	74369909	74369804	ID=MsG0280010751.01.T01:cds;Parent=MsG0280010751.01.T01
Chr2	CDS	74369354	74369754	74369354	ID=MsG0280010751.01.T01:cds;Parent=MsG0280010751.01.T01
Chr2	mRNA	74369200	74369725	74369200	ID=MsG0280010751.01.T02;Parent=MsG0280010751.01;Name=MsG0280010751.01.T02;_AED=0.36;_eAED=0.40;_QI=0\|0\|0\|1\|0\|0\|2\|0\|162
Chr2	exon	74369200	74369335	74369200	ID=MsG0280010751.01.T02:exon:7559;Parent=MsG0280010751.01.T02
Chr2	exon	74369373	74369725	74369373	ID=MsG0280010751.01.T02:exon:7558;Parent=MsG0280010751.01.T02
Chr2	CDS	74369373	74369725	74369373	ID=MsG0280010751.01.T02:cds;Parent=MsG0280010751.01.T02
Chr2	CDS	74369200	74369335	74369200	ID=MsG0280010751.01.T02:cds;Parent=MsG0280010751.01.T02
Chr2	mRNA	74369354	74369725	74369354	ID=MsG0280010751.01.T03;Parent=MsG0280010751.01;Name=MsG0280010751.01.T03;_AED=0.39;_eAED=0.39;_QI=0\|-1\|0\|1\|-1\|1\|1\|0\|123
Chr2	exon	74369354	74369725	74369354	ID=MsG0280010751.01.T03:exon:7560;Parent=MsG0280010751.01.T03
Chr2	CDS	74369354	74369725	74369354	ID=MsG0280010751.01.T03:cds;Parent=MsG0280010751.01.T03

Gene Sequence

>MsG0280010751.01.T02

ATGTATGAATTCAAAGTCCGCCGGTGCACTCGTAGCCGGAGCCATGACTGGACTGACTGTCCATTCGCTCACCCCGGTGAAAAAGCTCGCCGCCGTGATCCACGGCGGTTTCATTACTCAGGGACAGTTTGCCCTGAGTATCGCCGCGGTGGCTGTAACCGTGGCGATACTTGTGAATTTGCTCATGGTGTTTTTGAATGCTGGCTTCATCCTGCTAGATACAGAACTGAAGCATGCAAAGATGGTAAAAACTGTAAGAGAAAAGTTTGTTTCTTTGCACACACTCCTCGTCAATTAAGAATCCTTCCTGTTACATCATCATCATCTTCAAATGAACATTTTTCATGCAACAAATCAAACAATTGTTGTTTGTTTTGTCACTGTGGCGGTAATGGTAATGGTAATGGTGCTAATAATTCTCCAACTTCAACACTTTTTGGTATTCTCATTTTTCTTCGCCTCCGGCATCACCACCTATGTCTCCGATGA

>MsG0280010751.01.T03

>MsG0280010751.01.T01

CTACCAACCTCTCTCTCCCAAAAATCCCTTAGAGACATCGACATCCCTCCAAGAAAGCTCCTCACCCGCCGCAACACCACCGCCGCCGTCGACATCTTCTCCGACGATGATCCTTACTCATCAGATCATTTCCGAATGTATGAATTCAAAGTCCGCCGGTGCACTCGTAGCCGGAGCCATGACTGGACTGACTGTCCATTCGCTCACCCCGGTGAAAAAGCTCGCCGCCGTGATCCACGGCGGTTTCATTACTCAGGGACAGTTTGCCCTGAGTATCGCCGCGGTGGCTGTAACCGTGGCGATACTTGTGAATTTGCTCATGGTGTTTTTGAATGCTGGCTTCATCCTGCTAGATACAGAACTGAAGCATGCAAAGATGGTAAAAACTGTAAGAGAAAAGTTTGTTTCTTTGCACACACTCCTCGTCAATTAAGAATCCTTCCTGTTACATCATCATCATCTTCAAATGAACATTTTTCATGCAACAAGAAAATAACAAGCTTTTGA

Protein sequence

>MsG0280010751.01.T02

MYEFKVRRCTRSRSHDWTDCPFAHPGEKARRRDPRRFHYSGTVCPEYRRGGCNRGDTCEFAHGVFECWLHPARYRTEACKDGKNCKRKVCFFAHTPRQLRILPVTSSSSSNEHFSCNKSNNCCLFCHCGGNGNGNGANNSPTSTLFGILIFLRLRHHHLCLR*

>MsG0280010751.01.T03

MYEFKVRRCTRSRSHDWTDCPFAHPGEKARRRDPRRFHYSGTVCPEYRRGGCNRGDTCEFAHGVFECWLHPARYRTEACKDGKNCKRKVCFFAHTPRQLRILPVTSSSSSNEHFSCNKKITSF*

>MsG0280010751.01.T01

LPTSLSQKSLRDIDIPPRKLLTRRNTTAAVDIFSDDDPYSSDHFRMYEFKVRRCTRSRSHDWTDCPFAHPGEKARRRDPRRFHYSGTVCPEYRRGGCNRGDTCEFAHGVFECWLHPARYRTEACKDGKNCKRKVCFFAHTPRQLRILPVTSSSSSNEHFSCNKKITSF*