Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MsG0880047704.01.T01 | XP_003610522.1 | 92.143 | 140 | 10 | 1 | 1 | 139 | 1 | 140 | 1.58E-36 | 132 |
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MsG0880047704.01.T01 | G7JKJ2 | 92.143 | 140 | 10 | 1 | 1 | 139 | 1 | 140 | 7.55e-37 | 132 |
| Gene ID | Type | Classification |
|---|
| Gene ID | Type | Classification |
|---|
Co-expression Network
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|---|---|---|---|
| MsG0180000028.01 | MsG0880047704.01 | 0.816652 | 4.953268e-52 | 3.530479e-49 |
| MsG0180000466.01 | MsG0880047704.01 | 0.801919 | 7.172965e-49 | 3.467512e-46 |
| MsG0180001004.01 | MsG0880047704.01 | 0.801003 | 1.104961e-48 | 5.219794e-46 |
| MsG0180001169.01 | MsG0880047704.01 | 0.819055 | 1.419969e-52 | 1.081184e-49 |
| MsG0180002404.01 | MsG0880047704.01 | 0.815660 | 8.248037e-52 | 5.721559e-49 |
| MsG0880045356.01 | MsG0880047704.01 | 0.815341 | 9.714216e-52 | 6.679847e-49 |
| MsG0880047090.01 | MsG0880047704.01 | 0.800633 | 1.315087e-48 | 6.153843e-46 |
| MsG0880047450.01 | MsG0880047704.01 | 0.813987 | 1.937955e-51 | 1.284473e-48 |
| MsG0180003905.01 | MsG0880047704.01 | 0.835345 | 1.771540e-56 | 2.160498e-53 |
| MsG0180004064.01 | MsG0880047704.01 | 0.802785 | 4.758488e-49 | 2.351715e-46 |
| MsG0180004467.01 | MsG0880047704.01 | 0.855996 | 4.334050e-62 | 1.019377e-58 |
| MsG0180006183.01 | MsG0880047704.01 | 0.816019 | 6.860147e-52 | 4.805760e-49 |
| MsG0780040716.01 | MsG0880047704.01 | 0.807121 | 5.900091e-50 | 3.259149e-47 |
| MsG0780040978.01 | MsG0880047704.01 | 0.830613 | 2.661645e-55 | 2.818652e-52 |
| MsG0780040990.01 | MsG0880047704.01 | 0.805023 | 1.630800e-49 | 8.536067e-47 |
| MsG0780041823.01 | MsG0880047704.01 | 0.836899 | 7.137641e-57 | 9.120884e-54 |
| MsG0280010751.01 | MsG0880047704.01 | 0.815222 | 1.032504e-51 | 7.076109e-49 |
| MsG0380012222.01 | MsG0880047704.01 | 0.801172 | 1.020551e-48 | 4.841249e-46 |
| MsG0480019171.01 | MsG0880047704.01 | 0.801362 | 9.330431e-49 | 4.447499e-46 |
| MsG0480021740.01 | MsG0880047704.01 | 0.813043 | 3.124510e-51 | 2.018649e-48 |
| MsG0580024980.01 | MsG0880047704.01 | 0.818410 | 1.988767e-52 | 1.487649e-49 |
| MsG0580025633.01 | MsG0880047704.01 | 0.801242 | 9.874333e-49 | 4.692534e-46 |
| MsG0380015402.01 | MsG0880047704.01 | 0.826077 | 3.305728e-54 | 3.068426e-51 |
| MsG0380016250.01 | MsG0880047704.01 | 0.825379 | 4.840075e-54 | 4.404163e-51 |
| MsG0380016490.01 | MsG0880047704.01 | 0.816740 | 4.732824e-52 | 3.381354e-49 |
| MsG0380017588.01 | MsG0880047704.01 | 0.803437 | 3.486978e-49 | 1.752252e-46 |
| MsG0380017826.01 | MsG0880047704.01 | 0.800899 | 1.160622e-48 | 5.468083e-46 |
| MsG0580027854.01 | MsG0880047704.01 | 0.812508 | 4.092478e-51 | 2.606955e-48 |
| MsG0580028364.01 | MsG0880047704.01 | 0.801119 | 1.046325e-48 | 4.957029e-46 |
| MsG0580029218.01 | MsG0880047704.01 | 0.829838 | 4.113253e-55 | 4.260032e-52 |
PPI
| Gene1 | Gene2 | Type |
|---|
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MsG0880047704.01.T01 | MTR_4g133110 | 92.143 | 140 | 10 | 1 | 1 | 139 | 1 | 140 | 1.91e-40 | 132 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|
Find 44 sgRNAs with CRISPR-Local
Find 75 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| TACGGATTTATTATTATATA+AGG | 0.229977 | 8:+89814354 | MsG0880047704.01.T01:three_prime_UTR |
| CTCCACTGTTACTACTTCTT+CGG | 0.326738 | 8:-89813924 | None:intergenic |
| TATAGCCACTGAAGCTTGTT+TGG | 0.333899 | 8:+89814380 | MsG0880047704.01.T01:three_prime_UTR |
| TTATTGTGAAGGTAAGTCTT+TGG | 0.338777 | 8:+89814322 | MsG0880047704.01.T01:three_prime_UTR |
| AGTCTTTGGGTATGGCTTTA+CGG | 0.371673 | 8:+89814336 | MsG0880047704.01.T01:three_prime_UTR |
| TATTGTGAAGGTAAGTCTTT+GGG | 0.384333 | 8:+89814323 | MsG0880047704.01.T01:three_prime_UTR |
| TTACCTCAACAGTGGCCATT+TGG | 0.390008 | 8:-89813312 | None:intergenic |
| GTGGTGGTTACTTCTTCAAC+TGG | 0.408262 | 8:-89814184 | None:intergenic |
| CGGGTTCTCTACCTCTGCCT+TGG | 0.417414 | 8:-89814002 | None:intergenic |
| TTCTTCAACTGGTGCTGCTA+TGG | 0.423717 | 8:-89814173 | None:intergenic |
| TGTGTACTCTCAGCTTCAAC+TGG | 0.435451 | 8:-89813965 | None:intergenic |
| GAAATATGAACTAGCAAGAA+AGG | 0.445512 | 8:-89813266 | None:intergenic |
| TGGACATTATGTATTTCTCC+TGG | 0.447344 | 8:+89814400 | MsG0880047704.01.T01:three_prime_UTR |
| ACTACTTCTTCGGTGGTGGC+TGG | 0.451704 | 8:-89813914 | None:intergenic |
| TGTTACTACTTCTTCGGTGG+TGG | 0.470019 | 8:-89813918 | None:intergenic |
| TGAAGGTAAGTCTTTGGGTA+TGG | 0.473362 | 8:+89814328 | MsG0880047704.01.T01:three_prime_UTR |
| AGAAACTACTGAAACAGAAC+CGG | 0.474732 | 8:+89814075 | MsG0880047704.01.T01:CDS |
| ATTCATCTTTCCATTCCAAA+TGG | 0.477645 | 8:+89813297 | MsG0880047704.01.T01:exon |
| CAAGGCAGAGGTAGAGAACC+CGG | 0.480116 | 8:+89814003 | MsG0880047704.01.T01:CDS |
| CGGTGTTGTTTCCTCGACCT+TGG | 0.487832 | 8:-89813867 | None:intergenic |
| TGTCATTGATCTTATTGTGA+AGG | 0.517162 | 8:+89814311 | MsG0880047704.01.T01:three_prime_UTR |
| CACTGTTACTACTTCTTCGG+TGG | 0.532727 | 8:-89813921 | None:intergenic |
| GGTGGTGGCTGGTTCTGAAG+TGG | 0.534204 | 8:-89813903 | None:intergenic |
| AGAGGAAGTATCAGCTGAAG+AGG | 0.536609 | 8:+89814048 | MsG0880047704.01.T01:CDS |
| ATTCCAAATGGCCACTGTTG+AGG | 0.536707 | 8:+89813309 | MsG0880047704.01.T01:exon |
| AACTGAGGAAGCCAAGGCAG+AGG | 0.538217 | 8:+89813991 | MsG0880047704.01.T01:CDS |
| GAAGTAGTAACAGTGGAGAA+AGG | 0.540973 | 8:+89813929 | MsG0880047704.01.T01:CDS |
| TCAACAGTGGCCATTTGGAA+TGG | 0.543349 | 8:-89813307 | None:intergenic |
| GTGGTGGCTGGTTCTGAAGT+GGG | 0.546159 | 8:-89813902 | None:intergenic |
| GAAGTGGGAACCTCGGAGGC+CGG | 0.549296 | 8:-89813887 | None:intergenic |
| AGAGGAAGTATCAACTGAAG+AGG | 0.587702 | 8:+89814120 | MsG0880047704.01.T01:CDS |
| AAATATAATTTATTCAAACC+AGG | 0.594421 | 8:-89814418 | None:intergenic |
| TGGTTCTGAAGTGGGAACCT+CGG | 0.596316 | 8:-89813894 | None:intergenic |
| AATGTCCAAACAAGCTTCAG+TGG | 0.598179 | 8:-89814385 | None:intergenic |
| AGTTGAGGTAACCAAGGTCG+AGG | 0.604857 | 8:+89813856 | MsG0880047704.01.T01:CDS |
| GACAGAAAATGAAACAGTTG+AGG | 0.607247 | 8:+89813841 | MsG0880047704.01.T01:CDS |
| ACAAGTAACTGAGGAAGCCA+AGG | 0.616520 | 8:+89813985 | MsG0880047704.01.T01:CDS |
| TGAGAGTACACAAGTAACTG+AGG | 0.633074 | 8:+89813976 | MsG0880047704.01.T01:CDS |
| TGAAACAGTTGAGGTAACCA+AGG | 0.643241 | 8:+89813850 | MsG0880047704.01.T01:CDS |
| TTCTGAAGTGGGAACCTCGG+AGG | 0.645606 | 8:-89813891 | None:intergenic |
| GGAAACAACACCGGCCTCCG+AGG | 0.649162 | 8:+89813877 | MsG0880047704.01.T01:CDS |
| CACCGAAGAAGTAGTAACAG+TGG | 0.674611 | 8:+89813922 | MsG0880047704.01.T01:CDS |
| TCTTTGAATTACCTCAACAG+TGG | 0.681859 | 8:-89813320 | None:intergenic |
| CAAGGTCGAGGAAACAACAC+CGG | 0.700669 | 8:+89813868 | MsG0880047704.01.T01:CDS |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|---|---|---|---|---|
| !! | AAATATAATTTATTCAAACC+AGG | - | Chr8:89814421-89814440 | None:intergenic | 15.0% |
| !! | TACGGATTTATTATTATATA+AGG | + | Chr8:89814354-89814373 | MsG0880047704.01.T01:three_prime_UTR | 15.0% |
| !!! | AATTGATTATTGATCTTTTT+TGG | - | Chr8:89813588-89813607 | None:intergenic | 15.0% |
| !! | AAACAAAAATATTAGTCCAA+TGG | + | Chr8:89813508-89813527 | MsG0880047704.01.T01:intron | 20.0% |
| !! | TGGATTATGAAAAATAAACA+CGG | - | Chr8:89813568-89813587 | None:intergenic | 20.0% |
| !!! | ATTTGTTGTTTATAATCCAT+TGG | - | Chr8:89813527-89813546 | None:intergenic | 20.0% |
| ! | GATTATGAAAAATAAACACG+GGG | - | Chr8:89813566-89813585 | None:intergenic | 25.0% |
| ! | GGATTATGAAAAATAAACAC+GGG | - | Chr8:89813567-89813586 | None:intergenic | 25.0% |
| !!! | AATATTTTTGTTTGATCCAC+TGG | - | Chr8:89813501-89813520 | None:intergenic | 25.0% |
| ATATGACCAAGTAACTCATA+GGG | - | Chr8:89813741-89813760 | None:intergenic | 30.0% | |
| ATTCATCTTTCCATTCCAAA+TGG | + | Chr8:89813297-89813316 | MsG0880047704.01.T01:exon | 30.0% | |
| CTTGATGTTACAAATTCACA+CGG | + | Chr8:89813469-89813488 | MsG0880047704.01.T01:intron | 30.0% | |
| GAAATATGAACTAGCAAGAA+AGG | - | Chr8:89813269-89813288 | None:intergenic | 30.0% | |
| TATATGACCAAGTAACTCAT+AGG | - | Chr8:89813742-89813761 | None:intergenic | 30.0% | |
| TATTGTGAAGGTAAGTCTTT+GGG | + | Chr8:89814323-89814342 | MsG0880047704.01.T01:three_prime_UTR | 30.0% | |
| TGAAAAAGTTGAAGTGAAAG+AGG | + | Chr8:89814030-89814049 | MsG0880047704.01.T01:CDS | 30.0% | |
| TGTCATTGATCTTATTGTGA+AGG | + | Chr8:89814311-89814330 | MsG0880047704.01.T01:three_prime_UTR | 30.0% | |
| TTATTGTGAAGGTAAGTCTT+TGG | + | Chr8:89814322-89814341 | MsG0880047704.01.T01:three_prime_UTR | 30.0% | |
| ! | ACAGAAGCATAAAAAGTGAT+CGG | + | Chr8:89814205-89814224 | MsG0880047704.01.T01:exon | 30.0% |
| !! | TCTTTCACTTCAACTTTTTC+AGG | - | Chr8:89814031-89814050 | None:intergenic | 30.0% |
| !!! | GCTTTTCTGCTTTATTTGTT+TGG | + | Chr8:89814281-89814300 | MsG0880047704.01.T01:three_prime_UTR | 30.0% |
| AGAAACTACTGAAACAGAAC+CGG | + | Chr8:89814075-89814094 | MsG0880047704.01.T01:CDS | 35.0% | |
| AGCATAAAAAGTGATCGGAA+AGG | + | Chr8:89814210-89814229 | MsG0880047704.01.T01:exon | 35.0% | |
| CATAACAACATGTTGAGCAT+TGG | + | Chr8:89813764-89813783 | MsG0880047704.01.T01:intron | 35.0% | |
| GACAGAAAATGAAACAGTTG+AGG | + | Chr8:89813841-89813860 | MsG0880047704.01.T01:CDS | 35.0% | |
| TCTTTGAATTACCTCAACAG+TGG | - | Chr8:89813323-89813342 | None:intergenic | 35.0% | |
| TGGACATTATGTATTTCTCC+TGG | + | Chr8:89814400-89814419 | MsG0880047704.01.T01:three_prime_UTR | 35.0% | |
| TTAATGAAAATGGATCCTGC+AGG | + | Chr8:89813790-89813809 | MsG0880047704.01.T01:intron | 35.0% | |
| ! | CTCTTTTGCTTCGACTTTTT+CGG | - | Chr8:89814104-89814123 | None:intergenic | 35.0% |
| ! | GCATTGGTGGTTAATGAAAA+TGG | + | Chr8:89813780-89813799 | MsG0880047704.01.T01:intron | 35.0% |
| ! | TCATTTTCTGTCACAGTTGT+TGG | - | Chr8:89813833-89813852 | None:intergenic | 35.0% |
| !! | TCTTTTGCTTCGACTTTTTC+GGG | - | Chr8:89814103-89814122 | None:intergenic | 35.0% |
| !!! | ACTTCAACTTTTTCAGGTTC+CGG | - | Chr8:89814025-89814044 | None:intergenic | 35.0% |
| !!! | GATCACTTTTTATGCTTCTG+TGG | - | Chr8:89814206-89814225 | None:intergenic | 35.0% |
| AATGTCCAAACAAGCTTCAG+TGG | - | Chr8:89814388-89814407 | None:intergenic | 40.0% | |
| CATGTTCCCTATGAGTTACT+TGG | + | Chr8:89813732-89813751 | MsG0880047704.01.T01:intron | 40.0% | |
| CGAAAAAGTCGAAGCAAAAG+AGG | + | Chr8:89814102-89814121 | MsG0880047704.01.T01:CDS | 40.0% | |
| CTCCACTGTTACTACTTCTT+CGG | - | Chr8:89813927-89813946 | None:intergenic | 40.0% | |
| GAAGTAGTAACAGTGGAGAA+AGG | + | Chr8:89813929-89813948 | MsG0880047704.01.T01:CDS | 40.0% | |
| TGAAACAGTTGAGGTAACCA+AGG | + | Chr8:89813850-89813869 | MsG0880047704.01.T01:CDS | 40.0% | |
| TGAAGGTAAGTCTTTGGGTA+TGG | + | Chr8:89814328-89814347 | MsG0880047704.01.T01:three_prime_UTR | 40.0% | |
| ! | AGTCTTTGGGTATGGCTTTA+CGG | + | Chr8:89814336-89814355 | MsG0880047704.01.T01:three_prime_UTR | 40.0% |
| ! | TATAGCCACTGAAGCTTGTT+TGG | + | Chr8:89814380-89814399 | MsG0880047704.01.T01:three_prime_UTR | 40.0% |
| ! | TGAGAGTACACAAGTAACTG+AGG | + | Chr8:89813976-89813995 | MsG0880047704.01.T01:CDS | 40.0% |
| !! | AACAACATGTTGAGCATTGG+TGG | + | Chr8:89813767-89813786 | MsG0880047704.01.T01:intron | 40.0% |
| !! | AGAGGAAGTATCAACTGAAG+AGG | + | Chr8:89814120-89814139 | MsG0880047704.01.T01:CDS | 40.0% |
| !! | CACTTTTTATGCTTCTGTGG+TGG | - | Chr8:89814203-89814222 | None:intergenic | 40.0% |
| !!! | CTTCAACTTTTTCAGGTTCC+GGG | - | Chr8:89814024-89814043 | None:intergenic | 40.0% |
| ACAAGTAACTGAGGAAGCCA+AGG | + | Chr8:89813985-89814004 | MsG0880047704.01.T01:CDS | 45.0% | |
| ATTCCAAATGGCCACTGTTG+AGG | + | Chr8:89813309-89813328 | MsG0880047704.01.T01:exon | 45.0% | |
| CACCGAAGAAGTAGTAACAG+TGG | + | Chr8:89813922-89813941 | MsG0880047704.01.T01:CDS | 45.0% | |
| CACTGTTACTACTTCTTCGG+TGG | - | Chr8:89813924-89813943 | None:intergenic | 45.0% | |
| GTGGTGGTTACTTCTTCAAC+TGG | - | Chr8:89814187-89814206 | None:intergenic | 45.0% | |
| TCAACAGTGGCCATTTGGAA+TGG | - | Chr8:89813310-89813329 | None:intergenic | 45.0% | |
| TGTGTACTCTCAGCTTCAAC+TGG | - | Chr8:89813968-89813987 | None:intergenic | 45.0% | |
| TGTTACTACTTCTTCGGTGG+TGG | - | Chr8:89813921-89813940 | None:intergenic | 45.0% | |
| TTACCTCAACAGTGGCCATT+TGG | - | Chr8:89813315-89813334 | None:intergenic | 45.0% | |
| ! | ATTCACACGGCATTTTCCAG+TGG | + | Chr8:89813482-89813501 | MsG0880047704.01.T01:intron | 45.0% |
| ! | TTTGCTGTGTTTCAACCTGC+AGG | - | Chr8:89813808-89813827 | None:intergenic | 45.0% |
| !! | AGAGGAAGTATCAGCTGAAG+AGG | + | Chr8:89814048-89814067 | MsG0880047704.01.T01:CDS | 45.0% |
| !! | TTCTTCAACTGGTGCTGCTA+TGG | - | Chr8:89814176-89814195 | None:intergenic | 45.0% |
| AGTTGAGGTAACCAAGGTCG+AGG | + | Chr8:89813856-89813875 | MsG0880047704.01.T01:CDS | 50.0% | |
| CAAGGTCGAGGAAACAACAC+CGG | + | Chr8:89813868-89813887 | MsG0880047704.01.T01:CDS | 50.0% | |
| !! | TGGTTCTGAAGTGGGAACCT+CGG | - | Chr8:89813897-89813916 | None:intergenic | 50.0% |
| AACTGAGGAAGCCAAGGCAG+AGG | + | Chr8:89813991-89814010 | MsG0880047704.01.T01:CDS | 55.0% | |
| CAAGGCAGAGGTAGAGAACC+CGG | + | Chr8:89814003-89814022 | MsG0880047704.01.T01:CDS | 55.0% | |
| ! | ACTACTTCTTCGGTGGTGGC+TGG | - | Chr8:89813917-89813936 | None:intergenic | 55.0% |
| !! | CGGTGTTGTTTCCTCGACCT+TGG | - | Chr8:89813870-89813889 | None:intergenic | 55.0% |
| !! | GTGGTGGCTGGTTCTGAAGT+GGG | - | Chr8:89813905-89813924 | None:intergenic | 55.0% |
| !! | TTCTGAAGTGGGAACCTCGG+AGG | - | Chr8:89813894-89813913 | None:intergenic | 55.0% |
| !!! | GCTTCGACTTTTTCGGGTGC+CGG | - | Chr8:89814097-89814116 | None:intergenic | 55.0% |
| CGGGTTCTCTACCTCTGCCT+TGG | - | Chr8:89814005-89814024 | None:intergenic | 60.0% | |
| !! | GGTGGTGGCTGGTTCTGAAG+TGG | - | Chr8:89813906-89813925 | None:intergenic | 60.0% |
| GAAGTGGGAACCTCGGAGGC+CGG | - | Chr8:89813890-89813909 | None:intergenic | 65.0% | |
| GGAAACAACACCGGCCTCCG+AGG | + | Chr8:89813877-89813896 | MsG0880047704.01.T01:CDS | 65.0% |
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| Chr8 | gene | 89813234 | 89814448 | 89813234 | ID=MsG0880047704.01;Name=MsG0880047704.01 |
| Chr8 | mRNA | 89813234 | 89814448 | 89813234 | ID=MsG0880047704.01.T01;Parent=MsG0880047704.01;Name=MsG0880047704.01.T01;_AED=0.50;_eAED=0.50;_QI=82|1|1|1|1|1|2|232|139 |
| Chr8 | exon | 89813234 | 89813330 | 89813234 | ID=MsG0880047704.01.T01:exon:16073;Parent=MsG0880047704.01.T01 |
| Chr8 | exon | 89813812 | 89814448 | 89813812 | ID=MsG0880047704.01.T01:exon:16074;Parent=MsG0880047704.01.T01 |
| Chr8 | five_prime_UTR | 89813234 | 89813315 | 89813234 | ID=MsG0880047704.01.T01:five_prime_utr;Parent=MsG0880047704.01.T01 |
| Chr8 | CDS | 89813316 | 89813330 | 89813316 | ID=MsG0880047704.01.T01:cds;Parent=MsG0880047704.01.T01 |
| Chr8 | CDS | 89813812 | 89814216 | 89813812 | ID=MsG0880047704.01.T01:cds;Parent=MsG0880047704.01.T01 |
| Chr8 | three_prime_UTR | 89814217 | 89814448 | 89814217 | ID=MsG0880047704.01.T01:three_prime_utr;Parent=MsG0880047704.01.T01 |
| Gene Sequence |
| Protein sequence |
