AlfalfaGEDB | MsG0280010909.01

M. sativa cultivar ZhongmuNo.1 / MsG0280010909.01

KEGG: -
GO: -
PFAM: PF04520

Nr:

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Query id	Subject id	identity %	alignment length	mis matches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MsG0280010909.01.T01	XP_003597242.1	92.857	154	11	0	2	155	3	156	1.04E-94	281

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Swissprot:

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Query id	Subject id	identity %	alignment length	mis matches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
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Trembl:

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Query id	Subject id	identity %	alignment length	mis matches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MsG0280010909.01.T01	G7IRS7	92.857	154	11	0	2	155	3	156	4.97e-95	281

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TFs/TRs:

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Gene ID	Type	Classification
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Protein Kinases:

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Gene ID	Type	Classification
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Network:

Co-expression Network

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Gene1	Gene2	correlation coefficient	p_value	FDR
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PPI

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Gene1	Gene2	Type
MsG0280010909.01	MsG0780040929.01	PPI

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Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MsG0280010909.01.T01	MTR_2g094410	92.857	154	11	0	2	155	3	156	1.26e-98	281
MsG0280010909.01.T01	MTR_4g059320	59.740	154	51	6	10	154	7	158	2.35e-47	151
MsG0280010909.01.T01	MTR_7g101250	46.711	152	69	6	14	155	11	160	1.85e-27	100
MsG0280010909.01.T01	MTR_4g132020	58.333	84	28	2	71	153	97	174	4.00e-24	92.4
MsG0280010909.01.T01	MTR_2g023070	54.639	97	37	3	60	153	124	216	1.20e-23	92.4
MsG0280010909.01.T01	MTR_2g023390	54.651	86	31	2	72	153	73	154	6.72e-21	83.6
MsG0280010909.01.T01	MTR_3g052330	48.214	112	29	5	73	155	87	198	4.70e-20	82.4
MsG0280010909.01.T01	MTR_2g086660	45.545	101	42	3	63	155	160	255	7.71e-18	77.8
MsG0280010909.01.T01	MTR_2g086660	44.898	98	41	3	63	152	160	252	1.24e-16	75.1
MsG0280010909.01.T01	MTR_2g018175	51.163	86	28	4	71	153	50	124	8.01e-12	59.3

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Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MsG0280010909.01.T01	AT3G45210	54.487	156	62	4	1	155	1	148	4.30e-45	145
MsG0280010909.01.T01	AT5G60680	53.374	163	64	5	4	155	2	163	1.21e-40	134
MsG0280010909.01.T01	AT2G28400	44.785	163	77	3	4	155	2	162	9.49e-34	116
MsG0280010909.01.T01	AT5G03230	40.260	154	77	3	17	155	13	166	1.13e-30	108
MsG0280010909.01.T01	AT4G21970	50.000	86	38	2	72	153	59	143	2.37e-22	87.4
MsG0280010909.01.T01	AT4G04630	55.556	90	31	3	72	153	78	166	1.02e-21	86.3
MsG0280010909.01.T01	AT1G11700	49.451	91	34	3	71	153	113	199	1.70e-17	75.9
MsG0280010909.01.T01	AT1G61930	40.397	151	69	5	15	153	60	201	5.78e-16	72.0
MsG0280010909.01.T01	AT4G21930	38.125	160	83	5	8	153	24	181	2.38e-15	70.1
MsG0280010909.01.T01	AT3G15040	45.977	87	43	1	73	155	157	243	1.87e-13	65.9

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Find 28 sgRNAs with CRISPR-Local

Find 33 sgRNAs with CRISPR-GE

CRISPR-Local

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CRISPR-Local
sgRNA_sequence	on_target_score	Position	Region
AACAGAATTGCGAAACTCGG+GGG	0.678802	2:-76691738	None:intergenic
AATGCGATTTGGGCTAAAAC+TGG	0.386558	2:+76692034	MsG0280010909.01.T01:CDS
ACAGAATTGTTGTTTGTGGA+TGG	0.537414	2:-76691659	None:intergenic
AGTGACAGAATTGTTGTTTG+TGG	0.431710	2:-76691663	None:intergenic
ATCCATGCCAGTGAATGTTC+CGG	0.518219	2:+76691817	MsG0280010909.01.T01:CDS
ATCCGGAACATTCACTGGCA+TGG	0.543608	2:-76691819	None:intergenic
CCAAGTATCTTTGACCAATC+CGG	0.462167	2:-76691836	None:intergenic
CCGGATTGGTCAAAGATACT+TGG	0.412621	2:+76691836	MsG0280010909.01.T01:CDS
CGAACAGAATTGCGAAACTC+GGG	0.519518	2:-76691740	None:intergenic
CGGAGAAGAAACTACGATGA+AGG	0.537583	2:+76691878	MsG0280010909.01.T01:CDS

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CRISPR-GE

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sgRNA_sequence	Strand	Position	Region	GC_content
GTTTCTTGCTAGAACAAGAA+TGG	+	Chr2:76691946-76691965	MsG0280010909.01.T01:CDS	35.0%
CCAAGTATCTTTGACCAATC+CGG	-	Chr2:76691839-76691858	None:intergenic	40.0%
GAAAGGACGTGATCTTAGTA+GGG	+	Chr2:76692006-76692025	MsG0280010909.01.T01:CDS	40.0%
GTTCTAGCAAGAAACTCATG+CGG	-	Chr2:76691941-76691960	None:intergenic	40.0%
TAGGGTTCGAAATGCGATTT+GGG	+	Chr2:76692024-76692043	MsG0280010909.01.T01:CDS	40.0%
TGAAAGGACGTGATCTTAGT+AGG	+	Chr2:76692005-76692024	MsG0280010909.01.T01:CDS	40.0%
TGTTTGTGGATGGAAGAAAG+TGG	-	Chr2:76691652-76691671	None:intergenic	40.0%
AACAGAATTGCGAAACTCGG+GGG	-	Chr2:76691741-76691760	None:intergenic	45.0%
ATCCATGCCAGTGAATGTTC+CGG	+	Chr2:76691817-76691836	MsG0280010909.01.T01:CDS	45.0%
CGAACAGAATTGCGAAACTC+GGG	-	Chr2:76691743-76691762	None:intergenic	45.0%

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Chromosome	Type	Strat	End	Strand	Name
Chr2	gene	76691602	76692069	76691602	ID=MsG0280010909.01;Name=MsG0280010909.01
Chr2	mRNA	76691602	76692069	76691602	ID=MsG0280010909.01.T01;Parent=MsG0280010909.01;Name=MsG0280010909.01.T01;_AED=0.41;_eAED=0.41;_QI=0\|-1\|0\|1\|-1\|1\|1\|0\|155
Chr2	exon	76691602	76692069	76691602	ID=MsG0280010909.01.T01:exon:11280;Parent=MsG0280010909.01.T01
Chr2	CDS	76691602	76692069	76691602	ID=MsG0280010909.01.T01:cds;Parent=MsG0280010909.01.T01

Gene Sequence

>MsG0280010909.01.T01

ATGGCAACAACAACAACTAAGAACCACCATGTTCGAAAACAAACCTACCACTTTCTTCCATCCACAAACAACAATTCTGTCACTGATTCACTCTTCGAGTTCGACGAATCAGAACTCTACAACACTAACTCAAATTCCCCCGAGTTTCGCAATTCTGTTCGCGTTTCTCGTTTTCAAGGTACTTCATCATCTTCCACCACAGATGGTCGTGTGTCATCCATGCCAGTGAATGTTCCGGATTGGTCAAAGATACTTGGAGAAGAGTACAGACAGAATCGGAGAAGAAACTACGATGAAGGTGATGATGAAGATGAAGAAGATGACGAGAAAGTTCCACCGCATGAGTTTCTTGCTAGAACAAGAATGGCTTCATTTTCTGTTCATGAAGGTGTTGGTAGAACTTTGAAAGGACGTGATCTTAGTAGGGTTCGAAATGCGATTTGGGCTAAAACTGGTTTTCAAGACTAG

Protein sequence

>MsG0280010909.01.T01

MATTTTKNHHVRKQTYHFLPSTNNNSVTDSLFEFDESELYNTNSNSPEFRNSVRVSRFQGTSSSSTTDGRVSSMPVNVPDWSKILGEEYRQNRRRNYDEGDDEDEEDDEKVPPHEFLARTRMASFSVHEGVGRTLKGRDLSRVRNAIWAKTGFQD*