AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar ZhongmuNo.1 / MsG0780040929.01


Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MsG0780040929.01.T01 MTR_7g101250 94.578 166 3 2 1 166 1 160 4.48e-111 313
MsG0780040929.01.T01 MTR_2g094410 43.312 157 73 5 11 166 15 156 4.67e-31 110
MsG0780040929.01.T01 MTR_4g059320 47.368 133 62 4 36 165 31 158 2.27e-30 108
MsG0780040929.01.T01 MTR_4g132020 35.450 189 84 8 1 165 1 175 1.23e-21 86.7
MsG0780040929.01.T01 MTR_2g023070 47.059 85 40 2 82 165 137 217 2.03e-17 76.3
MsG0780040929.01.T01 MTR_2g023390 51.163 86 34 2 83 164 73 154 5.98e-16 71.2
MsG0780040929.01.T01 MTR_2g086660 40.230 87 47 2 84 166 170 255 5.09e-12 62.4
MsG0780040929.01.T01 MTR_3g052330 39.091 110 38 4 84 164 87 196 1.45e-11 60.5
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MsG0780040929.01.T01 AT5G03230 40.805 174 84 5 3 166 2 166 3.32e-32 113
MsG0780040929.01.T01 AT3G45210 43.636 165 74 4 2 166 3 148 1.24e-29 106
MsG0780040929.01.T01 AT2G28400 38.824 170 92 3 1 166 1 162 9.69e-28 102
MsG0780040929.01.T01 AT5G60680 43.023 172 74 5 6 166 5 163 1.55e-27 101
MsG0780040929.01.T01 AT4G21970 49.462 93 28 4 83 164 59 143 2.19e-17 75.1
MsG0780040929.01.T01 AT4G04630 38.298 141 74 4 37 164 26 166 7.56e-17 73.9
MsG0780040929.01.T01 AT1G11700 43.750 96 44 2 76 164 107 199 1.46e-15 71.2
MsG0780040929.01.T01 AT1G61930 46.154 91 39 2 82 164 113 201 5.06e-15 69.7
MsG0780040929.01.T01 AT4G26950 36.170 141 64 5 37 164 15 142 3.36e-14 66.2
MsG0780040929.01.T01 AT4G26950 36.170 141 64 5 37 164 15 142 3.36e-14 66.2
MsG0780040929.01.T01 AT4G21930 35.897 156 66 8 35 164 34 181 9.38e-14 66.2
MsG0780040929.01.T01 AT3G15040 41.379 87 47 1 84 166 157 243 2.35e-12 63.2
MsG0780040929.01.T01 AT1G29640 41.284 109 43 5 65 164 41 137 1.13e-11 59.7

Find 29 sgRNAs with CRISPR-Local

Find 36 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
GTCAATCAATGGCTTCTTCT+AGG 0.289420 7:+84396967 None:intergenic
GTATTCATCCTTCAAAATCT+TGG 0.290307 7:-84397252 None:intergenic
GGGAAAGGAAGGACTTTAAA+AGG 0.299267 7:+84397395 MsG0780040929.01.T01:CDS
GTAGTGTAAGGAATGCTATT+TGG 0.358519 7:+84397429 MsG0780040929.01.T01:CDS
ATGCTATTTGGAAGAAAGTT+GGG 0.379786 7:+84397441 MsG0780040929.01.T01:CDS
GACCAATCTGGTATATTCAT+TGG 0.380128 7:-84397230 None:intergenic
AATGCTATTTGGAAGAAAGT+TGG 0.385752 7:+84397440 MsG0780040929.01.T01:CDS
TTCAAAATCTTGGACCAATC+TGG 0.413413 7:-84397242 None:intergenic
CTAGAAACTCTCTTCAAACC+TGG 0.465204 7:-84397158 None:intergenic
GTTTCTGTCTCTGAGGTAGT+TGG 0.466042 7:-84397023 None:intergenic
AGTTTGATGAAGCTGATGTA+TGG 0.474055 7:+84397084 MsG0780040929.01.T01:CDS
GAAGAGACTTGCGTAGTGTA+AGG 0.480322 7:+84397417 MsG0780040929.01.T01:CDS
AAATGTAACTTGTTCTTGAA+AGG 0.483499 7:-84396998 None:intergenic
CTTTCTGTTCATGAAGGGAA+AGG 0.500406 7:+84397380 MsG0780040929.01.T01:CDS
GAAGATGAAGGTGATTATGA+TGG 0.512537 7:+84397302 MsG0780040929.01.T01:CDS
GCTTCTCTTTCTGTTCATGA+AGG 0.513560 7:+84397374 MsG0780040929.01.T01:CDS
CTTCTCTTTCTGTTCATGAA+GGG 0.517982 7:+84397375 MsG0780040929.01.T01:CDS
TACCAATGAATATACCAGAT+TGG 0.518610 7:+84397228 MsG0780040929.01.T01:CDS
AGTAGTGATGATGAAGATGA+AGG 0.546106 7:+84397290 MsG0780040929.01.T01:CDS
GGTAATGAAGATGAAGCTAA+AGG 0.550449 7:-84397209 None:intergenic
CTGTTCATGAAGGGAAAGGA+AGG 0.560984 7:+84397384 MsG0780040929.01.T01:CDS
GAATATCTTCTTAGAACTAG+AGG 0.587771 7:+84397350 MsG0780040929.01.T01:CDS
TGAAGGTGATTATGATGGAG+GGG 0.591476 7:+84397307 MsG0780040929.01.T01:CDS
GAAATTTGTTTCTGTCTCTG+AGG 0.611033 7:-84397030 None:intergenic
GATGAAGGTGATTATGATGG+AGG 0.617604 7:+84397305 MsG0780040929.01.T01:CDS
ATGAAGGTGATTATGATGGA+GGG 0.625231 7:+84397306 MsG0780040929.01.T01:CDS
CTAAGAAGATATTCATGAGG+AGG 0.629545 7:-84397341 None:intergenic
AGGTGATTATGATGGAGGGG+TGG 0.659515 7:+84397310 MsG0780040929.01.T01:CDS
GTTCTAAGAAGATATTCATG+AGG 0.678087 7:-84397344 None:intergenic

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
! TTCAATCAAAAATCATCACA+AGG + Chr7:84397050-84397069 MsG0780040929.01.T01:CDS 25.0%
!!! AAATGTAACTTGTTCTTGAA+AGG - Chr7:84397001-84397020 None:intergenic 25.0%
ACAAACATAGAGCCAAAAAA+GGG + Chr7:84397131-84397150 MsG0780040929.01.T01:CDS 30.0%
GAATATCTTCTTAGAACTAG+AGG + Chr7:84397350-84397369 MsG0780040929.01.T01:CDS 30.0%
GTATTCATCCTTCAAAATCT+TGG - Chr7:84397255-84397274 None:intergenic 30.0%
TACCAATGAATATACCAGAT+TGG + Chr7:84397228-84397247 MsG0780040929.01.T01:CDS 30.0%
! AATGCTATTTGGAAGAAAGT+TGG + Chr7:84397440-84397459 MsG0780040929.01.T01:CDS 30.0%
! ATGCTATTTGGAAGAAAGTT+GGG + Chr7:84397441-84397460 MsG0780040929.01.T01:CDS 30.0%
! GAAGAGAGTTTCTAGAAAAA+TGG + Chr7:84397166-84397185 MsG0780040929.01.T01:CDS 30.0%
!! GTTCTAAGAAGATATTCATG+AGG - Chr7:84397347-84397366 None:intergenic 30.0%
AGTTTGATGAAGCTGATGTA+TGG + Chr7:84397084-84397103 MsG0780040929.01.T01:CDS 35.0%
CACAAACATAGAGCCAAAAA+AGG + Chr7:84397130-84397149 MsG0780040929.01.T01:CDS 35.0%
CTAAGAAGATATTCATGAGG+AGG - Chr7:84397344-84397363 None:intergenic 35.0%
CTTCTCTTTCTGTTCATGAA+GGG + Chr7:84397375-84397394 MsG0780040929.01.T01:CDS 35.0%
GAAGATGAAGGTGATTATGA+TGG + Chr7:84397302-84397321 MsG0780040929.01.T01:CDS 35.0%
GACCAATCTGGTATATTCAT+TGG - Chr7:84397233-84397252 None:intergenic 35.0%
GGTAATGAAGATGAAGCTAA+AGG - Chr7:84397212-84397231 None:intergenic 35.0%
GTAGTGTAAGGAATGCTATT+TGG + Chr7:84397429-84397448 MsG0780040929.01.T01:CDS 35.0%
TTCAAAATCTTGGACCAATC+TGG - Chr7:84397245-84397264 None:intergenic 35.0%
! AGTAGTGATGATGAAGATGA+AGG + Chr7:84397290-84397309 MsG0780040929.01.T01:CDS 35.0%
! ATGAAGGTGATTATGATGGA+GGG + Chr7:84397306-84397325 MsG0780040929.01.T01:CDS 35.0%
!! AGATTGGTCCAAGATTTTGA+AGG + Chr7:84397244-84397263 MsG0780040929.01.T01:CDS 35.0%
!! GAAATTTGTTTCTGTCTCTG+AGG - Chr7:84397033-84397052 None:intergenic 35.0%
!!! TTTTGGCTCTATGTTTGTGT+TGG - Chr7:84397129-84397148 None:intergenic 35.0%
CTAGAAACTCTCTTCAAACC+TGG - Chr7:84397161-84397180 None:intergenic 40.0%
CTTTCTGTTCATGAAGGGAA+AGG + Chr7:84397380-84397399 MsG0780040929.01.T01:CDS 40.0%
GATGAAGGTGATTATGATGG+AGG + Chr7:84397305-84397324 MsG0780040929.01.T01:CDS 40.0%
GCTTCTCTTTCTGTTCATGA+AGG + Chr7:84397374-84397393 MsG0780040929.01.T01:CDS 40.0%
GGGAAAGGAAGGACTTTAAA+AGG + Chr7:84397395-84397414 MsG0780040929.01.T01:CDS 40.0%
! TGAAGGTGATTATGATGGAG+GGG + Chr7:84397307-84397326 MsG0780040929.01.T01:CDS 40.0%
!!! AAACCTGGTACACCCTTTTT+TGG - Chr7:84397146-84397165 None:intergenic 40.0%
CTGTTCATGAAGGGAAAGGA+AGG + Chr7:84397384-84397403 MsG0780040929.01.T01:CDS 45.0%
GAAGAGACTTGCGTAGTGTA+AGG + Chr7:84397417-84397436 MsG0780040929.01.T01:CDS 45.0%
!! GTTTCTGTCTCTGAGGTAGT+TGG - Chr7:84397026-84397045 None:intergenic 45.0%
! AGGTGATTATGATGGAGGGG+TGG + Chr7:84397310-84397329 MsG0780040929.01.T01:CDS 50.0%
! GAGCCAAAAAAGGGTGTACC+AGG + Chr7:84397140-84397159 MsG0780040929.01.T01:CDS 50.0%
Chromosome Type Strat End Strand Name
Chr7 gene 84396975 84397475 84396975 ID=MsG0780040929.01;Name=MsG0780040929.01
Chr7 mRNA 84396975 84397475 84396975 ID=MsG0780040929.01.T01;Parent=MsG0780040929.01;Name=MsG0780040929.01.T01;_AED=0.50;_eAED=0.50;_QI=0|-1|0|1|-1|1|1|0|166
Chr7 exon 84396975 84397475 84396975 ID=MsG0780040929.01.T01:exon:36633;Parent=MsG0780040929.01.T01
Chr7 CDS 84396975 84397475 84396975 ID=MsG0780040929.01.T01:cds;Parent=MsG0780040929.01.T01
Gene Sequence

>MsG0780040929.01.T01

ATGGCTTCTTCTAGGAAGAGTTTCCTTTCAAGAACAAGTTACATTTTTCCAACTACCTCAGAGACAGAAACAAATTTCAATCAAAAATCATCACAAGGAAAAGAATTAGAGTTTGATGAAGCTGATGTATGGAACATGTCATATTCAAATTCCAACACAAACATAGAGCCAAAAAAGGGTGTACCAGGTTTGAAGAGAGTTTCTAGAAAAATGGAATCTAATAATAAAGTTAATCCTTTAGCTTCATCTTCATTACCAATGAATATACCAGATTGGTCCAAGATTTTGAAGGATGAATACAAAAAGAAGAAAGAGAGTAGTGATGATGAAGATGAAGGTGATTATGATGGAGGGGTGGTTCAGTTACCTCCTCATGAATATCTTCTTAGAACTAGAGGAGCTTCTCTTTCTGTTCATGAAGGGAAAGGAAGGACTTTAAAAGGAAGAGACTTGCGTAGTGTAAGGAATGCTATTTGGAAGAAAGTTGGGTTTGAAGATTGA

Protein sequence

>MsG0780040929.01.T01

MASSRKSFLSRTSYIFPTTSETETNFNQKSSQGKELEFDEADVWNMSYSNSNTNIEPKKGVPGLKRVSRKMESNNKVNPLASSSLPMNIPDWSKILKDEYKKKKESSDDEDEGDYDGGVVQLPPHEYLLRTRGASLSVHEGKGRTLKGRDLRSVRNAIWKKVGFED*